LOCUS       DADQRX010000001        91165 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.001, whole genome shotgun sequence.
ACCESSION   DADQRX010000001 DADQRX010000000
VERSION     DADQRX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 91165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.001"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(218..1015)
                     /locus_tag="J8V14_000001"
     CDS             complement(218..1015)
                     /locus_tag="J8V14_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBA0920145.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="J8V14_000002"
     CDS             complement(1012..2196)
                     /locus_tag="J8V14_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBA0920146.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="J8V14_000003"
     CDS             complement(2221..3468)
                     /locus_tag="J8V14_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HBA0920147.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="J8V14_000004"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="J8V14_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBA0920148.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="J8V14_000005"
     CDS             5448..5762
                     /locus_tag="J8V14_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBA0920149.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="J8V14_000006"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="J8V14_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBA0920150.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="J8V14_000007"
     CDS             6447..7091
                     /locus_tag="J8V14_000007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBA0920151.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="J8V14_000008"
     CDS             7106..7435
                     /locus_tag="J8V14_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920152.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="J8V14_000009"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="J8V14_000009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBA0920153.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="J8V14_000010"
     CDS             8423..9247
                     /locus_tag="J8V14_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBA0920154.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="J8V14_000011"
     CDS             9240..9587
                     /locus_tag="J8V14_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBA0920155.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="J8V14_000012"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="J8V14_000012"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBA0920156.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="J8V14_000013"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="J8V14_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBA0920157.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="J8V14_000014"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="J8V14_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBA0920158.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="J8V14_000015"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="J8V14_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBA0920159.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="J8V14_000016"
     CDS             15244..16011
                     /locus_tag="J8V14_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920160.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="J8V14_000017"
     CDS             16001..18043
                     /locus_tag="J8V14_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920161.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="J8V14_000018"
     CDS             18324..18473
                     /locus_tag="J8V14_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBA0920162.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="J8V14_000019"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="J8V14_000019"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBA0920163.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="J8V14_000020"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="J8V14_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBA0920164.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="J8V14_000021"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="J8V14_000021"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBA0920165.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="J8V14_000022"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="J8V14_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBA0920166.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="J8V14_000023"
     CDS             22890..23972
                     /locus_tag="J8V14_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBA0920167.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="J8V14_000024"
     CDS             23972..24775
                     /locus_tag="J8V14_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBA0920168.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="J8V14_000025"
     CDS             24845..25585
                     /locus_tag="J8V14_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA0920169.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="J8V14_000026"
     CDS             25596..26600
                     /locus_tag="J8V14_000026"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBA0920170.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="J8V14_000027"
     CDS             complement(26645..28489)
                     /locus_tag="J8V14_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920171.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28700..29218
                     /locus_tag="J8V14_000028"
     CDS             28700..29218
                     /locus_tag="J8V14_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA0920172.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29346..30029
                     /locus_tag="J8V14_000029"
     CDS             29346..30029
                     /locus_tag="J8V14_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBA0920173.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30338..30411
                     /locus_tag="J8V14_000030"
     tRNA            30338..30411
                     /locus_tag="J8V14_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30372..30374,aa:Arg,seq:tct)
     gene            30570..30929
                     /locus_tag="J8V14_000031"
     CDS             30570..30929
                     /locus_tag="J8V14_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBA0920174.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31052..32683
                     /locus_tag="J8V14_000032"
     CDS             31052..32683
                     /locus_tag="J8V14_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBA0920175.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32745..33386)
                     /locus_tag="J8V14_000033"
     CDS             complement(32745..33386)
                     /locus_tag="J8V14_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBA0920176.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33668..33976
                     /gene="rplU"
                     /locus_tag="J8V14_000034"
     CDS             33668..33976
                     /gene="rplU"
                     /locus_tag="J8V14_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBA0920177.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34003..34347
                     /locus_tag="J8V14_000035"
     CDS             34003..34347
                     /locus_tag="J8V14_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBA0920178.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34360..34653
                     /gene="rpmA"
                     /locus_tag="J8V14_000036"
     CDS             34360..34653
                     /gene="rpmA"
                     /locus_tag="J8V14_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBA0920179.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35060..37894
                     /locus_tag="J8V14_000037"
     CDS             35060..37894
                     /locus_tag="J8V14_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0920180.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38085..38576
                     /locus_tag="J8V14_000038"
     CDS             38085..38576
                     /locus_tag="J8V14_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HBA0920181.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38679..39668
                     /gene="manX"
                     /locus_tag="J8V14_000039"
     CDS             38679..39668
                     /gene="manX"
                     /locus_tag="J8V14_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBA0920182.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39701..40504
                     /locus_tag="J8V14_000040"
     CDS             39701..40504
                     /locus_tag="J8V14_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA0920183.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40525..41439
                     /locus_tag="J8V14_000041"
     CDS             40525..41439
                     /locus_tag="J8V14_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBA0920184.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41522..41896
                     /locus_tag="J8V14_000042"
     CDS             41522..41896
                     /locus_tag="J8V14_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBA0920185.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42035..42280
                     /locus_tag="J8V14_000043"
     CDS             42035..42280
                     /locus_tag="J8V14_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA0920186.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42452..43516
                     /locus_tag="J8V14_000044"
     CDS             42452..43516
                     /locus_tag="J8V14_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBA0920187.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43762..44187
                     /locus_tag="J8V14_000045"
     CDS             43762..44187
                     /locus_tag="J8V14_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBA0920188.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44180..44629
                     /gene="nusB"
                     /locus_tag="J8V14_000046"
     CDS             44180..44629
                     /gene="nusB"
                     /locus_tag="J8V14_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBA0920189.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44726..45580
                     /locus_tag="J8V14_000047"
     CDS             44726..45580
                     /locus_tag="J8V14_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBA0920190.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45581..46918
                     /locus_tag="J8V14_000048"
     CDS             45581..46918
                     /locus_tag="J8V14_000048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBA0920191.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46923..47147
                     /locus_tag="J8V14_000049"
     CDS             46923..47147
                     /locus_tag="J8V14_000049"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBA0920192.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47149..48039
                     /locus_tag="J8V14_000050"
     CDS             47149..48039
                     /locus_tag="J8V14_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBA0920193.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48048..48866
                     /locus_tag="J8V14_000051"
     CDS             48048..48866
                     /locus_tag="J8V14_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBA0920194.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48973..49422
                     /locus_tag="J8V14_000052"
     CDS             48973..49422
                     /locus_tag="J8V14_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBA0920195.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49440..51122
                     /gene="recN"
                     /locus_tag="J8V14_000053"
     CDS             49440..51122
                     /gene="recN"
                     /locus_tag="J8V14_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBA0920196.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51177..51293)
                     /locus_tag="J8V14_000054"
     CDS             complement(51177..51293)
                     /locus_tag="J8V14_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBA0920197.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51366..52322)
                     /locus_tag="J8V14_000055"
     CDS             complement(51366..52322)
                     /locus_tag="J8V14_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBA0920198.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52493..52861
                     /locus_tag="J8V14_000056"
     CDS             52493..52861
                     /locus_tag="J8V14_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBA0920199.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53031..53462
                     /gene="mraZ"
                     /locus_tag="J8V14_000057"
     CDS             53031..53462
                     /gene="mraZ"
                     /locus_tag="J8V14_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBA0920200.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53483..54442
                     /gene="rsmH"
                     /locus_tag="J8V14_000058"
     CDS             53483..54442
                     /gene="rsmH"
                     /locus_tag="J8V14_000058"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBA0920201.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54447..54848
                     /gene="ftsL"
                     /locus_tag="J8V14_000059"
     CDS             54447..54848
                     /gene="ftsL"
                     /locus_tag="J8V14_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBA0920202.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54849..57041
                     /locus_tag="J8V14_000060"
     CDS             54849..57041
                     /locus_tag="J8V14_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBA0920203.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57067..58029
                     /locus_tag="J8V14_000061"
     CDS             57067..58029
                     /locus_tag="J8V14_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBA0920204.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58043..59404
                     /locus_tag="J8V14_000062"
     CDS             58043..59404
                     /locus_tag="J8V14_000062"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBA0920205.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59430..60518
                     /gene="murG"
                     /locus_tag="J8V14_000063"
     CDS             59430..60518
                     /gene="murG"
                     /locus_tag="J8V14_000063"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBA0920206.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60533..61753
                     /locus_tag="J8V14_000064"
     CDS             60533..61753
                     /locus_tag="J8V14_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBA0920207.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61909..63234
                     /gene="ftsA"
                     /locus_tag="J8V14_000065"
     CDS             61909..63234
                     /gene="ftsA"
                     /locus_tag="J8V14_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBA0920208.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63257..64498
                     /gene="ftsZ"
                     /locus_tag="J8V14_000066"
     CDS             63257..64498
                     /gene="ftsZ"
                     /locus_tag="J8V14_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBA0920209.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64515..65192
                     /locus_tag="J8V14_000067"
     CDS             64515..65192
                     /locus_tag="J8V14_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBA0920210.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65206..65805
                     /locus_tag="J8V14_000068"
     CDS             65206..65805
                     /locus_tag="J8V14_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBA0920211.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65820..66107
                     /locus_tag="J8V14_000069"
     CDS             65820..66107
                     /locus_tag="J8V14_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBA0920212.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66156..66938
                     /locus_tag="J8V14_000070"
     CDS             66156..66938
                     /locus_tag="J8V14_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBA0920213.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66959..67666
                     /locus_tag="J8V14_000071"
     CDS             66959..67666
                     /locus_tag="J8V14_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBA0920214.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67928..70726
                     /gene="ileS"
                     /locus_tag="J8V14_000072"
     CDS             67928..70726
                     /gene="ileS"
                     /locus_tag="J8V14_000072"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBA0920215.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70775..72301)
                     /locus_tag="J8V14_000073"
     CDS             complement(70775..72301)
                     /locus_tag="J8V14_000073"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBA0920216.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72316..72963)
                     /locus_tag="J8V14_000074"
     CDS             complement(72316..72963)
                     /locus_tag="J8V14_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBA0920217.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73147..73476)
                     /locus_tag="J8V14_000075"
     CDS             complement(73147..73476)
                     /locus_tag="J8V14_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBA0920218.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73653..74381
                     /gene="sfsA"
                     /locus_tag="J8V14_000076"
     CDS             73653..74381
                     /gene="sfsA"
                     /locus_tag="J8V14_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBA0920219.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74397..75410
                     /locus_tag="J8V14_000077"
     CDS             74397..75410
                     /locus_tag="J8V14_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0920220.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75410..76687
                     /locus_tag="J8V14_000078"
     CDS             75410..76687
                     /locus_tag="J8V14_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA0920221.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76750..79452
                     /locus_tag="J8V14_000079"
     CDS             76750..79452
                     /locus_tag="J8V14_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0920222.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAEWMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79604..79921
                     /locus_tag="J8V14_000080"
     CDS             79604..79921
                     /locus_tag="J8V14_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0920223.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79951..80271
                     /locus_tag="J8V14_000081"
     CDS             79951..80271
                     /locus_tag="J8V14_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBA0920224.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80379..81839
                     /locus_tag="J8V14_000082"
     CDS             80379..81839
                     /locus_tag="J8V14_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0920225.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81907..82128
                     /locus_tag="J8V14_000083"
     CDS             81907..82128
                     /locus_tag="J8V14_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920226.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82159..82341
                     /locus_tag="J8V14_000084"
     CDS             82159..82341
                     /locus_tag="J8V14_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBA0920227.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82341..82754
                     /locus_tag="J8V14_000085"
     CDS             82341..82754
                     /locus_tag="J8V14_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBA0920228.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82877..84058
                     /locus_tag="J8V14_000086"
     CDS             82877..84058
                     /locus_tag="J8V14_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920229.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84447..84520
                     /locus_tag="J8V14_000087"
     tRNA            84447..84520
                     /locus_tag="J8V14_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84481..84483,aa:Arg,seq:cct)
     gene            complement(84589..85728)
                     /locus_tag="J8V14_000088"
     CDS             complement(84589..85728)
                     /locus_tag="J8V14_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0920230.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(86027..86662)
                     /locus_tag="J8V14_000089"
     CDS             complement(86027..86662)
                     /locus_tag="J8V14_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920231.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(86775..87410)
                     /locus_tag="J8V14_000090"
     CDS             complement(86775..87410)
                     /locus_tag="J8V14_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBA0920232.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(87444..87905)
                     /locus_tag="J8V14_000091"
     CDS             complement(87444..87905)
                     /locus_tag="J8V14_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HBA0920233.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(88035..88466)
                     /locus_tag="J8V14_000092"
     CDS             complement(88035..88466)
                     /locus_tag="J8V14_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBA0920234.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(88484..88804)
                     /locus_tag="J8V14_000093"
     CDS             complement(88484..88804)
                     /locus_tag="J8V14_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0920235.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            89103..89879
                     /locus_tag="J8V14_000094"
     CDS             89103..89879
                     /locus_tag="J8V14_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBA0920236.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            89894..90097
                     /locus_tag="J8V14_000095"
     CDS             89894..90097
                     /locus_tag="J8V14_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0920237.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            90113..90451
                     /locus_tag="J8V14_000096"
     CDS             90113..90451
                     /locus_tag="J8V14_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBA0920238.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            90438..90617
                     /locus_tag="J8V14_000097"
     CDS             90438..90617
                     /locus_tag="J8V14_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920239.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(90660..91130)
                     /locus_tag="J8V14_000098"
     CDS             complement(90660..91130)
                     /locus_tag="J8V14_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920240.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT        29662 a        15549 c        18996 g        26958 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    28681 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    28741 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    28801 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    28861 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    28921 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    28981 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    29041 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    29101 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    29161 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    29221 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    29281 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    29341 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    29401 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    29461 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    29521 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    29581 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    29641 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    29701 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    29761 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    29821 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    29881 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    29941 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    30001 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    30061 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    30121 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    30181 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    30241 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    30301 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    30361 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    30421 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    30481 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    30541 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    30601 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    30661 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    30721 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    30781 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    30841 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    30901 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    30961 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    31021 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    31081 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    31141 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    31201 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    31261 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    31321 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    31381 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    31441 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    31501 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    31561 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    31621 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    31681 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    31741 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    31801 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    31861 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    31921 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    31981 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    32041 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    32101 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    32161 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    32221 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    32281 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    32341 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    32401 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    32461 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    32521 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    32581 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    32641 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    32701 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    32761 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    32821 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    32881 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    32941 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    33001 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    33061 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    33121 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    33181 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    33241 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    33301 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    33361 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    33421 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    33481 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    33541 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    33601 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    33661 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    33721 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    33781 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    33841 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    33901 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    33961 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    34021 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    34081 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    34141 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    34201 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    34261 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    34321 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    34381 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    34441 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    34501 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    34561 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    34621 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    34681 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    34741 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    34801 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    34861 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    34921 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    34981 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    35041 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    35101 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    35161 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    35221 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    35281 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    35341 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    35401 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    35461 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    35521 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    35581 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    35641 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    35701 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    35761 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    35821 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    35881 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    35941 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    36001 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    36061 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    36121 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    36181 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    36241 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    36301 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    36361 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    36421 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    36481 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    36541 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    36601 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    36661 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    36721 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    36781 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    36841 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    36901 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    36961 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    37021 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    37081 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    37141 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    37201 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    37261 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    37321 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    37381 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    37441 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    37501 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    37561 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    37621 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    37681 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    37741 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    37801 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    37861 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    37921 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    37981 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    38041 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    38101 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    38161 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    38221 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    38281 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    38341 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    38401 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    38461 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    38521 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    38581 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    38641 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    38701 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    38761 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    38821 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    38881 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    38941 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    39001 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    39061 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    39121 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    39181 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    39241 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    39301 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    39361 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    39421 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    39481 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    39541 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    39601 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    39661 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    39721 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    39781 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    39841 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    39901 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    39961 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    40021 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    40081 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    40141 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    40201 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    40261 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    40321 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    40381 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    40441 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    40501 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    40561 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    40621 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    40681 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    40741 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    40801 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    40861 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    40921 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    40981 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    41041 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    41101 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    41161 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    41221 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    41281 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    41341 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    41401 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    41461 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    41521 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    41581 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    41641 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    41701 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    41761 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    41821 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    41881 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    41941 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    42001 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    42061 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    42121 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    42181 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    42241 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    42301 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    42361 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    42421 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    42481 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    42541 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    42601 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    42661 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    42721 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    42781 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    42841 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    42901 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    42961 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    43021 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    43081 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    43141 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    43201 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    43261 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    43321 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    43381 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    43441 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    43501 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    43561 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    43621 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    43681 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    43741 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    43801 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    43861 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    43921 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    43981 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    44041 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    44101 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    44161 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    44221 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    44281 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    44341 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    44401 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    44461 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    44521 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    44581 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    44641 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    44701 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    44761 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    44821 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    44881 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    44941 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    45001 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    45061 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    45121 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    45181 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    45241 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    45301 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    45361 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    45421 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    45481 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    45541 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    45601 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    45661 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    45721 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    45781 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    45841 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    45901 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    45961 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    46021 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    46081 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    46141 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    46201 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    46261 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    46321 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    46381 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    46441 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    46501 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    46561 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    46621 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    46681 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    46741 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    46801 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    46861 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    46921 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    46981 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    47041 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    47101 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    47161 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    47221 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    47281 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    47341 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    47401 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    47461 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    47521 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    47581 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    47641 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    47701 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    47761 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    47821 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    47881 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    47941 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    48001 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    48061 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    48121 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    48181 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    48241 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    48301 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    48361 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    48421 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    48481 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    48541 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    48601 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    48661 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    48721 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    48781 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    48841 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    48901 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    48961 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    49021 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    49081 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    49141 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    49201 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    49261 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    49321 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    49381 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    49441 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    49501 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    49561 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    49621 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    49681 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    49741 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    49801 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    49861 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    49921 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    49981 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    50041 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    50101 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    50161 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    50221 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    50281 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    50341 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    50401 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    50461 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    50521 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    50581 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    50641 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    50701 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    50761 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    50821 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    50881 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    50941 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    51001 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    51061 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    51121 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    51181 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    51241 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    51301 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    51361 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    51421 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    51481 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    51541 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    51601 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    51661 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    51721 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    51781 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    51841 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    51901 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    51961 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    52021 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    52081 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    52141 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    52201 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    52261 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    52321 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    52381 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    52441 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    52501 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    52561 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    52621 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    52681 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    52741 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    52801 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    52861 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    52921 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    52981 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    53041 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    53101 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    53161 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    53221 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    53281 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    53341 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    53401 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    53461 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    53521 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    53581 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    53641 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    53701 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    53761 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    53821 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    53881 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    53941 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    54001 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    54061 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    54121 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    54181 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    54241 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    54301 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    54361 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    54421 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    54481 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    54541 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    54601 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    54661 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    54721 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    54781 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    54841 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    54901 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    54961 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    55021 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    55081 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    55141 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    55201 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    55261 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    55321 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    55381 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    55441 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    55501 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    55561 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    55621 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    55681 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    55741 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    55801 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    55861 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    55921 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    55981 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    56041 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    56101 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    56161 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    56221 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    56281 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    56341 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    56401 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    56461 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    56521 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    56581 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    56641 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    56701 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    56761 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    56821 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    56881 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    56941 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    57001 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    57061 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    57121 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    57181 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    57241 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    57301 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    57361 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    57421 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    57481 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    57541 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    57601 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    57661 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    57721 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    57781 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    57841 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    57901 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    57961 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    58021 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    58081 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    58141 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    58201 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    58261 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    58321 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    58381 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    58441 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    58501 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    58561 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    58621 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    58681 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    58741 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    58801 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    58861 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    58921 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    58981 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    59041 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    59101 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    59161 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    59221 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    59281 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    59341 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    59401 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    59461 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    59521 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    59581 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    59641 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    59701 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    59761 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    59821 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    59881 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    59941 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    60001 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    60061 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    60121 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    60181 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    60241 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    60301 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    60361 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    60421 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    60481 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    60541 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    60601 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    60661 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    60721 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    60781 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    60841 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    60901 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    60961 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    61021 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    61081 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    61141 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    61201 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    61261 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    61321 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    61381 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    61441 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    61501 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    61561 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    61621 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    61681 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    61741 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    61801 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    61861 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    61921 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    61981 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    62041 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    62101 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    62161 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    62221 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    62281 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    62341 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    62401 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    62461 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    62521 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    62581 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    62641 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    62701 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    62761 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    62821 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    62881 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    62941 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    63001 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    63061 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    63121 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    63181 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    63241 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    63301 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    63361 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    63421 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    63481 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    63541 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    63601 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    63661 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    63721 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    63781 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    63841 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    63901 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    63961 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    64021 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    64081 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    64141 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    64201 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    64261 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    64321 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    64381 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    64441 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    64501 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    64561 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    64621 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    64681 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    64741 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    64801 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    64861 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    64921 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    64981 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    65041 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    65101 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    65161 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    65221 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    65281 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    65341 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    65401 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    65461 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    65521 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    65581 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    65641 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    65701 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    65761 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    65821 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    65881 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    65941 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    66001 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    66061 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    66121 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    66181 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    66241 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    66301 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    66361 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    66421 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    66481 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    66541 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    66601 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    66661 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    66721 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    66781 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    66841 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    66901 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    66961 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    67021 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    67081 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    67141 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    67201 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    67261 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    67321 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    67381 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    67441 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    67501 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    67561 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    67621 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    67681 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    67741 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    67801 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    67861 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    67921 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    67981 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    68041 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    68101 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    68161 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    68221 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    68281 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    68341 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    68401 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    68461 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    68521 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    68581 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    68641 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    68701 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    68761 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    68821 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    68881 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    68941 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    69001 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    69061 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    69121 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    69181 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    69241 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    69301 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    69361 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    69421 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    69481 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    69541 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    69601 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    69661 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    69721 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    69781 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    69841 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    69901 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    69961 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    70021 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    70081 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    70141 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    70201 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    70261 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    70321 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    70381 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    70441 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    70501 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    70561 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    70621 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    70681 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    70741 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    70801 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    70861 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    70921 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    70981 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    71041 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    71101 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    71161 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    71221 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    71281 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    71341 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    71401 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    71461 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    71521 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    71581 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    71641 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    71701 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    71761 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    71821 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    71881 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    71941 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    72001 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    72061 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    72121 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    72181 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    72241 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    72301 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    72361 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    72421 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    72481 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    72541 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    72601 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    72661 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    72721 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    72781 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    72841 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    72901 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    72961 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    73021 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    73081 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    73141 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    73201 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    73261 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    73321 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    73381 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    73441 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    73501 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    73561 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    73621 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    73681 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    73741 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    73801 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    73861 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    73921 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    73981 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    74041 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    74101 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    74161 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    74221 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    74281 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    74341 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    74401 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    74461 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    74521 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    74581 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    74641 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    74701 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    74761 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    74821 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    74881 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    74941 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    75001 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    75061 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    75121 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    75181 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    75241 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    75301 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    75361 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    75421 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    75481 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    75541 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    75601 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    75661 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    75721 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    75781 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    75841 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    75901 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    75961 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    76021 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    76081 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    76141 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    76201 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    76261 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    76321 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    76381 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    76441 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    76501 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    76561 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    76621 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    76681 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    76741 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    76801 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    76861 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    76921 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    76981 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    77041 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    77101 acagggaagt cgatgtttgc tgaatggatg tatcattttg caatcgattc agagatgctt
    77161 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgatcaaaa tccgcaactg
    77221 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    77281 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    77341 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    77401 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    77461 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    77521 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    77581 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    77641 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    77701 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    77761 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    77821 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    77881 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    77941 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    78001 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    78061 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    78121 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    78181 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    78241 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    78301 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    78361 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    78421 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    78481 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    78541 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    78601 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    78661 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    78721 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    78781 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    78841 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    78901 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    78961 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    79021 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    79081 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    79141 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    79201 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    79261 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    79321 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    79381 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    79441 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    79501 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    79561 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    79621 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    79681 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    79741 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    79801 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    79861 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    79921 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    79981 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    80041 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    80101 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    80161 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    80221 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    80281 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    80341 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    80401 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    80461 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    80521 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    80581 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    80641 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    80701 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    80761 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    80821 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    80881 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    80941 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    81001 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    81061 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    81121 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    81181 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    81241 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    81301 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    81361 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    81421 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    81481 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    81541 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    81601 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    81661 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    81721 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    81781 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    81841 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    81901 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    81961 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    82021 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    82081 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    82141 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    82201 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    82261 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    82321 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    82381 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    82441 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    82501 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    82561 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    82621 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    82681 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    82741 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    82801 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    82861 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    82921 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    82981 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    83041 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    83101 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    83161 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    83221 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    83281 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    83341 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    83401 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    83461 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    83521 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
    83581 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
    83641 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
    83701 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
    83761 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
    83821 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
    83881 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
    83941 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
    84001 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
    84061 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
    84121 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
    84181 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
    84241 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
    84301 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
    84361 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
    84421 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
    84481 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
    84541 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
    84601 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
    84661 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
    84721 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
    84781 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
    84841 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
    84901 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
    84961 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
    85021 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
    85081 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
    85141 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
    85201 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
    85261 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
    85321 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
    85381 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
    85441 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
    85501 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
    85561 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
    85621 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
    85681 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
    85741 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
    85801 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
    85861 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
    85921 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
    85981 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
    86041 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
    86101 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
    86161 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
    86221 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
    86281 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
    86341 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
    86401 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
    86461 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
    86521 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
    86581 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
    86641 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
    86701 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
    86761 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
    86821 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
    86881 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
    86941 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
    87001 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
    87061 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
    87121 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
    87181 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
    87241 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
    87301 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
    87361 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
    87421 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
    87481 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
    87541 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
    87601 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
    87661 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
    87721 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
    87781 gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct aataattgaa
    87841 atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa aatctctttt
    87901 tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc atatatgagg
    87961 aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
    88021 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
    88081 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
    88141 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
    88201 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
    88261 tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc atgagataaa
    88321 tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat tctgggtttt
    88381 ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt gtattcgctc
    88441 aacatttctt tcaatttcaa atacatacaa accatcactc caactattca ttttcctcta
    88501 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
    88561 aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct ctttcttctc
    88621 tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt aattcatctg
    88681 aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca aaattaattt
    88741 ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt tcataggtat
    88801 tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa tacgcagaaa
    88861 taatttgact aattctgagt aatgagctat actatatctt gtaaacagat ttaacaacta
    88921 aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa gctttaaaat
    88981 caatgttttt atgtcttatt taattatgct cttagtatag agtattactc agatactgtc
    89041 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
    89101 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
    89161 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
    89221 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
    89281 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
    89341 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
    89401 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
    89461 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
    89521 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
    89581 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
    89641 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
    89701 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
    89761 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
    89821 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
    89881 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
    89941 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
    90001 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
    90061 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
    90121 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
    90181 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
    90241 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
    90301 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
    90361 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
    90421 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
    90481 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
    90541 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
    90601 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
    90661 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
    90721 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
    90781 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
    90841 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
    90901 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
    90961 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
    91021 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
    91081 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
    91141 attgttaagt gaaaaaaatt caaaa
//
LOCUS       DADQRX010000002        78903 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.002, whole genome shotgun sequence.
ACCESSION   DADQRX010000002 DADQRX010000000
VERSION     DADQRX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 78903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.002"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..386
                     /locus_tag="J8V14_000099"
     CDS             <1..386
                     /locus_tag="J8V14_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0920241.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(691..1005)
                     /locus_tag="J8V14_000100"
                     /pseudo
     CDS             complement(691..1005)
                     /locus_tag="J8V14_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(1259..2894)
                     /locus_tag="J8V14_000101"
                     /pseudo
     CDS             complement(1259..2894)
                     /locus_tag="J8V14_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3217..3696)
                     /gene="rlmH"
                     /locus_tag="J8V14_000102"
     CDS             complement(3217..3696)
                     /gene="rlmH"
                     /locus_tag="J8V14_000102"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBA0920242.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4124..5413
                     /locus_tag="J8V14_000103"
     CDS             4124..5413
                     /locus_tag="J8V14_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBA0920243.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(5452..6057)
                     /locus_tag="J8V14_000104"
     CDS             complement(5452..6057)
                     /locus_tag="J8V14_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBA0920244.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(6262..6726)
                     /locus_tag="J8V14_000105"
     CDS             complement(6262..6726)
                     /locus_tag="J8V14_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBA0920245.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6748..7068)
                     /locus_tag="J8V14_000106"
     CDS             complement(6748..7068)
                     /locus_tag="J8V14_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBA0920246.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(7212..8291)
                     /gene="pepA"
                     /locus_tag="J8V14_000107"
     CDS             complement(7212..8291)
                     /gene="pepA"
                     /locus_tag="J8V14_000107"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBA0920247.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            8491..8841
                     /locus_tag="J8V14_000108"
     CDS             8491..8841
                     /locus_tag="J8V14_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBA0920248.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            9064..10011
                     /locus_tag="J8V14_000109"
     CDS             9064..10011
                     /locus_tag="J8V14_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA0920249.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(10068..11486)
                     /gene="pepV"
                     /locus_tag="J8V14_000110"
     CDS             complement(10068..11486)
                     /gene="pepV"
                     /locus_tag="J8V14_000110"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBA0920250.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11635..12480
                     /locus_tag="J8V14_000111"
     CDS             11635..12480
                     /locus_tag="J8V14_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBA0920251.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12484..12924
                     /locus_tag="J8V14_000112"
     CDS             12484..12924
                     /locus_tag="J8V14_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBA0920252.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12974..13168
                     /locus_tag="J8V14_000113"
     CDS             12974..13168
                     /locus_tag="J8V14_000113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920253.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13304..13990
                     /locus_tag="J8V14_000114"
     CDS             13304..13990
                     /locus_tag="J8V14_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0920254.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(14398..15087)
                     /locus_tag="J8V14_000115"
     CDS             complement(14398..15087)
                     /locus_tag="J8V14_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920255.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(15062..17191)
                     /locus_tag="J8V14_000116"
     CDS             complement(15062..17191)
                     /locus_tag="J8V14_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBA0920256.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(17239..18576)
                     /locus_tag="J8V14_000117"
     CDS             complement(17239..18576)
                     /locus_tag="J8V14_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBA0920257.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18828..19187)
                     /gene="rplT"
                     /locus_tag="J8V14_000118"
     CDS             complement(18828..19187)
                     /gene="rplT"
                     /locus_tag="J8V14_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBA0920258.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(19240..19440)
                     /gene="rpmI"
                     /locus_tag="J8V14_000119"
     CDS             complement(19240..19440)
                     /gene="rpmI"
                     /locus_tag="J8V14_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBA0920259.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(19512..20036)
                     /gene="infC"
                     /locus_tag="J8V14_000120"
     CDS             complement(19512..20036)
                     /gene="infC"
                     /locus_tag="J8V14_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBA0920260.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            20356..20988
                     /locus_tag="J8V14_000121"
     CDS             20356..20988
                     /locus_tag="J8V14_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBA0920261.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20990..21895)
                     /locus_tag="J8V14_000122"
     CDS             complement(20990..21895)
                     /locus_tag="J8V14_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBA0920262.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21980..22633)
                     /locus_tag="J8V14_000123"
     CDS             complement(21980..22633)
                     /locus_tag="J8V14_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBA0920263.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            22955..23899
                     /gene="mvk"
                     /locus_tag="J8V14_000124"
     CDS             22955..23899
                     /gene="mvk"
                     /locus_tag="J8V14_000124"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBA0920264.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23892..24869
                     /gene="mvaD"
                     /locus_tag="J8V14_000125"
     CDS             23892..24869
                     /gene="mvaD"
                     /locus_tag="J8V14_000125"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBA0920265.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24882..25967
                     /locus_tag="J8V14_000126"
     CDS             24882..25967
                     /locus_tag="J8V14_000126"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBA0920266.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25964..27028
                     /locus_tag="J8V14_000127"
     CDS             25964..27028
                     /locus_tag="J8V14_000127"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBA0920267.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(27277..28080)
                     /locus_tag="J8V14_000128"
     CDS             complement(27277..28080)
                     /locus_tag="J8V14_000128"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBA0920268.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(28100..28942)
                     /gene="kduI"
                     /locus_tag="J8V14_000129"
     CDS             complement(28100..28942)
                     /gene="kduI"
                     /locus_tag="J8V14_000129"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBA0920269.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28958..29965)
                     /locus_tag="J8V14_000130"
     CDS             complement(28958..29965)
                     /locus_tag="J8V14_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA0920270.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29972..30616)
                     /locus_tag="J8V14_000131"
     CDS             complement(29972..30616)
                     /locus_tag="J8V14_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBA0920271.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30716..31474)
                     /locus_tag="J8V14_000132"
     CDS             complement(30716..31474)
                     /locus_tag="J8V14_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA0920272.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(31601..32752)
                     /gene="fucO"
                     /locus_tag="J8V14_000133"
     CDS             complement(31601..32752)
                     /gene="fucO"
                     /locus_tag="J8V14_000133"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBA0920273.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32834..33145)
                     /gene="rhaM"
                     /locus_tag="J8V14_000134"
     CDS             complement(32834..33145)
                     /gene="rhaM"
                     /locus_tag="J8V14_000134"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBA0920274.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(33147..33992)
                     /gene="rhaD"
                     /locus_tag="J8V14_000135"
     CDS             complement(33147..33992)
                     /gene="rhaD"
                     /locus_tag="J8V14_000135"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBA0920275.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(34006..35262)
                     /gene="rhaA"
                     /locus_tag="J8V14_000136"
     CDS             complement(34006..35262)
                     /gene="rhaA"
                     /locus_tag="J8V14_000136"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBA0920276.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(35259..36719)
                     /gene="rhaB"
                     /locus_tag="J8V14_000137"
     CDS             complement(35259..36719)
                     /gene="rhaB"
                     /locus_tag="J8V14_000137"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBA0920277.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36872..37741
                     /locus_tag="J8V14_000138"
     CDS             36872..37741
                     /locus_tag="J8V14_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA0920278.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37867..38766)
                     /locus_tag="J8V14_000139"
     CDS             complement(37867..38766)
                     /locus_tag="J8V14_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA0920279.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38931..39755)
                     /locus_tag="J8V14_000140"
     CDS             complement(38931..39755)
                     /locus_tag="J8V14_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA0920280.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(39766..41061)
                     /locus_tag="J8V14_000141"
     CDS             complement(39766..41061)
                     /locus_tag="J8V14_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBA0920281.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(41077..41556)
                     /locus_tag="J8V14_000142"
     CDS             complement(41077..41556)
                     /locus_tag="J8V14_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA0920282.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(41562..42590)
                     /locus_tag="J8V14_000143"
     CDS             complement(41562..42590)
                     /locus_tag="J8V14_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBA0920283.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(42607..43713)
                     /locus_tag="J8V14_000144"
     CDS             complement(42607..43713)
                     /locus_tag="J8V14_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBA0920284.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43942..46539)
                     /gene="adhE"
                     /locus_tag="J8V14_000145"
                     /gene_synonym="adhC"
     CDS             complement(43942..46539)
                     /gene="adhE"
                     /locus_tag="J8V14_000145"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBA0920285.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(46844..47224)
                     /gene="yajC"
                     /locus_tag="J8V14_000146"
     CDS             complement(46844..47224)
                     /gene="yajC"
                     /locus_tag="J8V14_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBA0920286.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(47321..48466)
                     /gene="tgt"
                     /locus_tag="J8V14_000147"
     CDS             complement(47321..48466)
                     /gene="tgt"
                     /locus_tag="J8V14_000147"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBA0920287.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            48684..49382
                     /locus_tag="J8V14_000148"
     CDS             48684..49382
                     /locus_tag="J8V14_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBA0920288.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(49550..52189)
                     /locus_tag="J8V14_000149"
     CDS             complement(49550..52189)
                     /locus_tag="J8V14_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBA0920289.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(52808..53839)
                     /gene="queA"
                     /locus_tag="J8V14_000150"
     CDS             complement(52808..53839)
                     /gene="queA"
                     /locus_tag="J8V14_000150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBA0920290.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            54032..55206
                     /locus_tag="J8V14_000151"
                     /pseudo
     CDS             54032..55206
                     /locus_tag="J8V14_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            55210..55851
                     /locus_tag="J8V14_000152"
     CDS             55210..55851
                     /locus_tag="J8V14_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920291.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            55848..56765
                     /locus_tag="J8V14_000153"
     CDS             55848..56765
                     /locus_tag="J8V14_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0920292.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            56762..57433
                     /locus_tag="J8V14_000154"
     CDS             56762..57433
                     /locus_tag="J8V14_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920293.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57974..58462)
                     /locus_tag="J8V14_000155"
     CDS             complement(57974..58462)
                     /locus_tag="J8V14_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBA0920294.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(58663..58929)
                     /locus_tag="J8V14_000156"
     CDS             complement(58663..58929)
                     /locus_tag="J8V14_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBA0920295.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58929..59192)
                     /locus_tag="J8V14_000157"
     CDS             complement(58929..59192)
                     /locus_tag="J8V14_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBA0920296.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(59185..59457)
                     /locus_tag="J8V14_000158"
     CDS             complement(59185..59457)
                     /locus_tag="J8V14_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920297.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            59668..59853
                     /locus_tag="J8V14_000159"
     CDS             59668..59853
                     /locus_tag="J8V14_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0920298.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(59995..61818)
                     /locus_tag="J8V14_000160"
     CDS             complement(59995..61818)
                     /locus_tag="J8V14_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBA0920299.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(62416..65121)
                     /locus_tag="J8V14_000161"
     CDS             complement(62416..65121)
                     /locus_tag="J8V14_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBA0920300.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(65427..66374)
                     /locus_tag="J8V14_000162"
     CDS             complement(65427..66374)
                     /locus_tag="J8V14_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBA0920301.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            66564..66833
                     /gene="rpsN"
                     /locus_tag="J8V14_000163"
     CDS             66564..66833
                     /gene="rpsN"
                     /locus_tag="J8V14_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBA0920302.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(67009..67371)
                     /locus_tag="J8V14_000164"
     CDS             complement(67009..67371)
                     /locus_tag="J8V14_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBA0920303.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(67382..68503)
                     /locus_tag="J8V14_000165"
     CDS             complement(67382..68503)
                     /locus_tag="J8V14_000165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBA0920304.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(68519..68869)
                     /locus_tag="J8V14_000166"
     CDS             complement(68519..68869)
                     /locus_tag="J8V14_000166"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBA0920305.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(68999..70510)
                     /locus_tag="J8V14_000167"
     CDS             complement(68999..70510)
                     /locus_tag="J8V14_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA0920306.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(70832..72184)
                     /locus_tag="J8V14_000168"
     CDS             complement(70832..72184)
                     /locus_tag="J8V14_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBA0920307.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(72337..73413)
                     /locus_tag="J8V14_000169"
     CDS             complement(72337..73413)
                     /locus_tag="J8V14_000169"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBA0920308.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            73609..75354
                     /locus_tag="J8V14_000170"
     CDS             73609..75354
                     /locus_tag="J8V14_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBA0920309.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(75634..76071)
                     /locus_tag="J8V14_000171"
     CDS             complement(75634..76071)
                     /locus_tag="J8V14_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBA0920310.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(76055..77428)
                     /locus_tag="J8V14_000172"
     CDS             complement(76055..77428)
                     /locus_tag="J8V14_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920311.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(77750..78238)
                     /locus_tag="J8V14_000173"
     CDS             complement(77750..78238)
                     /locus_tag="J8V14_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920312.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(78260..78862)
                     /locus_tag="J8V14_000174"
     CDS             complement(78260..78862)
                     /locus_tag="J8V14_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA0920313.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22764 a        16767 c        14364 g        25008 t
ORIGIN      
        1 atcaattaga aagcaacttt tttcgggatc taaatgaaat tctctcttat gatccagccc
       61 atagctatga ggaactatac actcgcttga ttgactatgt ttacgcaaat acttttttat
      121 atcgcttatt tatcctcaac catggtgata tttcgtttca gaagaaagtt aaacaagtta
      181 ttgaagaaaa ctatctgaac atatggctat atgaagacaa gaaaacgatt atcacagaag
      241 aaatgagatt ctttactacg taccacgtcc aaggttgtct atccgttctc aatcggtggg
      301 tagaaagcaa ttacaagtac tccaaaaaag aattattaga actcttacag aaactgaatg
      361 atcacataga gaaaatcatg ccttgaataa aaacatacta agcttagtta agaagaaccg
      421 taaccttctc gtataacaac tgaatcttat ttagccaaat tgtttactga agattttttg
      481 agtaaaattt ctataattcc tacttattga aaagtagttt gtagtgctgc ttctccctct
      541 ctaagtgata tatcttgttg taccaatcaa attcacctca tcattaataa attactttta
      601 ggctaattta ttacaactta taggttgaag ctgtaaccaa tttatagtca tattatattt
      661 ttatcatcca gtttattgaa ttcttctcgt ttacctcaat tcattttcaa ggggaatcag
      721 ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt taaatggttt
      781 cgcttctgga tgtttggctt tatatccttc gtaatcctta ctgttgataa ttgctttcat
      841 attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta attttttctc
      901 agaaacaaac cactcattgg aaaagacatg aatcaaatca tcacttactt gtgtaaaata
      961 gcgacgttta atgacaaaga tatcttcatt gctatctagt gtaccttcat ttctttgtgc
     1021 ttaaattcaa agataatttt ctcaataaag atttttgatt ccagtaagat tcctttactc
     1081 cttttacaac atagcgttgt cctttgtttt gaaacatctt cttcccctct ttgccattaa
     1141 aaatagccca attctctata aagaagatta agcttcatta ttataatata aagtttaaat
     1201 ttggtttatt acttactttt tcttattctc tagtaagcta atattgaaaa aaatgtaatc
     1261 agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa agaaagaggt
     1321 aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg gaagaaactt
     1381 caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc aatctctctt
     1441 ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag cgttattttt
     1501 tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg ataaggcata
     1561 gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt gtattgtaat
     1621 gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta acgaataaga
     1681 ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc atagtttgct
     1741 tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt aactactttt
     1801 ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga gccaaacagt
     1861 acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt attttgtaat
     1921 aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact aaggtaaatc
     1981 actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa agaaataata
     2041 ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat caaactatcc
     2101 gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat cgattgaacc
     2161 atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt cattcctact
     2221 gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc acttactaca
     2281 aagccgccta agactagtaa gccaacggct attacactca taaaagtgga aagaacatgt
     2341 gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt tttctcataa
     2401 atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat cgcttcagca
     2461 atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata taaacagcta
     2521 aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa catcagcatg
     2581 atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa attattactt
     2641 atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata attcactccg
     2701 ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga agataaccct
     2761 ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg aacatttcta
     2821 aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa tattattttt
     2881 ttctttactt gcattactta attatatact cctttcatgg attgctttat ctagtataac
     2941 ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca ctaaaattat
     3001 aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat tgcttaacta
     3061 atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt gcgatctctt
     3121 tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg gatatgacta
     3181 tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag gcgcccccgc
     3241 attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg
     3301 atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag
     3361 tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc
     3421 gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac
     3481 gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct
     3541 catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat
     3601 ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc
     3661 ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg
     3721 ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt
     3781 aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt
     3841 tattttacct tatttcatca cattcattag gcaaacatac gttttataag aatttatcca
     3901 ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt
     3961 tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt
     4021 cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac
     4081 tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt
     4141 gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct
     4201 tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac
     4261 tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt
     4321 ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga
     4381 cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact
     4441 attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga
     4501 aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca
     4561 cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc
     4621 tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa
     4681 agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat
     4741 tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc
     4801 ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc
     4861 gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga
     4921 aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa
     4981 tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa
     5041 tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt
     5101 atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa
     5161 cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca
     5221 atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc
     5281 tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa
     5341 agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa
     5401 tagtgaaaat taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg
     5461 aaatgcttct ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc
     5521 atttggcaaa tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa
     5581 agttcctggc cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac
     5641 tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc
     5701 tacttctgtc tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac
     5761 gtacccgaca acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc
     5821 tttgatcaat cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc
     5881 ttcgattgtt agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc
     5941 atctttgatc cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc
     6001 ggctataata accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt
     6061 ctttcctcca ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga
     6121 aatttttttc atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca
     6181 agggactgtg acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa
     6241 ggtcgtacaa gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa
     6301 gacatcacaa ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc
     6361 gactgcattt agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa
     6421 tttgactgcc atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta
     6481 atcaacagat ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg
     6541 ctgatcgact agattttgat caataggtgc atagcccata aagttataaa attgctgatc
     6601 gattacgttt gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt
     6661 gaaagcagca agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg
     6721 attcatcttg acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc
     6781 gatttcctgt tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg
     6841 gatcacgaca aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt
     6901 gtcacggtct acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg
     6961 cggtttgata aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc
     7021 agtttctacg tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct
     7081 actttctgtt tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat
     7141 tgatcctctt tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt
     7201 tcactatcgc attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc
     7261 agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga
     7321 tgcgtatgga tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt
     7381 gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg
     7441 tttgtttcag cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga
     7501 tcttggatac gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact
     7561 gtatcatctg ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca
     7621 gaagctttag aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta
     7681 tgacctaatg tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag
     7741 cggttgtccc atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg
     7801 taaggaacga ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca
     7861 aacccagcat cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc
     7921 aataaatgtg ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta
     7981 agtgtaaaac gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta
     8041 aaaagcccgt tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg
     8101 accattacac ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa
     8161 ccatctaact gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca
     8221 tcttgttcaa aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt
     8281 ttttcttcca tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga
     8341 tctgcggata aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt
     8401 ctggtagcgg ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag
     8461 taacaagtga gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt
     8521 aaaggcgggt tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt
     8581 tggtatcatc gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct
     8641 tttctaaaag aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg
     8701 aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa
     8761 actgtttatt atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga
     8821 aaaaaaggtg caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat
     8881 tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc
     8941 aaacatatat tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat
     9001 ttcctttgat tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga
     9061 aaaatggaac aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca
     9121 atttcaacag tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat
     9181 gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa
     9241 aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc
     9301 gactattatt taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg
     9361 ctcgagaaat tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga
     9421 acctactttg atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat
     9481 acaattacta gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc
     9541 aatcgtatct ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa
     9601 ttgacgatcc atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt
     9661 gatcgtcctg aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc
     9721 atgatgatta ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc
     9781 atcttcttct ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg
     9841 acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca
     9901 cttctttcat tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa
     9961 tatgaattga atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat
    10021 caagactgca aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa
    10081 cgatagatgg catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat
    10141 tcgtttgctt gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca
    10201 cgtttcaaca aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca
    10261 gtgtggtctt catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa
    10321 tgtggttcca ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt
    10381 tcgatgtcac tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg
    10441 atgaagttcg cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc
    10501 aatttgccca ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca
    10561 tggacataag cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta
    10621 tctaagaaca atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt
    10681 gcgcctttac caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt
    10741 actggatttg cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca
    10801 cttggtactt gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa
    10861 acaaaagatt tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa
    10921 acatttcctt tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct
    10981 ggttcttctt catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg
    11041 ccgactacga agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc
    11101 gcatagtaag ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt
    11161 ccatccttga ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca
    11221 tccatgtgtc caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca
    11281 tagttatcga cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc
    11341 aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc
    11401 tcgctgttga cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct
    11461 acttcttttt gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt
    11521 tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta
    11581 tctgtttttc tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg
    11641 ataaacgaac aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga
    11701 acatttggac gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg
    11761 agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa
    11821 aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact
    11881 gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt
    11941 acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa
    12001 tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc
    12061 tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta
    12121 ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg
    12181 ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct
    12241 ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca
    12301 ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat
    12361 agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc
    12421 gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga
    12481 gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg
    12541 gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg
    12601 gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg
    12661 gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca
    12721 actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa
    12781 gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa
    12841 cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct
    12901 tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg
    12961 agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat
    13021 gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct
    13081 cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa
    13141 accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg
    13201 catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga
    13261 tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt
    13321 gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg
    13381 aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta caccgattca
    13441 agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac
    13501 ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt
    13561 ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact
    13621 agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt
    13681 tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg
    13741 ggattggcta gaaagttata gtacgcactt gaaccgtttc agatggttac gtttgacgat
    13801 ttcagaatta gactggggtc gagtattaga tgaacatcag acaatgcttc aatcaatgat
    13861 tgatcataac tttgatgaag tcggatttct ttgcacgatg catttacata tgattatcga
    13921 ggagcaagaa tacgtgattc ataattaccc cgattatttt gaagatattc aggacagacc
    13981 catcaaataa gaggttttgg aatcagcgtt ttgacctgag tattagagaa agaaacggca
    14041 aaatagttcc tcatattttg ccgttttttt tgagctaata ccgagggtca gctgattgca
    14101 caccgtttat tgagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag
    14161 taaaaatagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    14221 gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc tccgagtcac aaggaacaat
    14281 ttcaaaaaac agcttctcat gtttgttgaa tattttgact tgtgaccgag gagttgacag
    14341 gagaacatcg tttattcgga aaaaaaggac tgtgacacga cttttgtcac agtctcttta
    14401 tttgtaatct aaatcatact ccgtacatct tttttctatt ttttttattt cttctaagat
    14461 cccttggcta cgaatttcat tatatggcca aagtaagact gcctctaata cataaggcga
    14521 aaaatcgctt tgattcatct cttttgaaaa actcatcgct gctatctcgc tcaatgcacg
    14581 aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt
    14641 ataaattccc ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc
    14701 ttgcgctgtt tctttagaga tcgaagctag tcctgaacat tttaattttt ctaacggctc
    14761 cgtcatcaat cgaatccctt catcttctgc ataagttgca aaagtgattc gttcgtttat
    14821 ttgctctgat tcacaggaaa cgattggaac agccatttgt tcacaaattt ctcctacatc
    14881 ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg ataccacaat cgattgattg
    14941 ttgtataaga attttctgct tggacaaagg aatctttttc caaaataaat ctcgccgaaa
    15001 ctggtagaat ccccaaattt cttcggggat ctgttgtaat tcgtttagta actcttctaa
    15061 cttatgtttg tttgatattt ccggcaacga cgatcacttc ttcctccggt tccatagtag
    15121 atacttccag atccatcaaa agctgtagtt gttccagatc cgtcgctgta aaatcagaga
    15181 ctcttgctcc taagcagaga taaaattctg gtcgggcaat caattcagtt tgataaactg
    15241 ccatatcttc ccaaagttgt tcgacagctg tcatataatt ggctgcagtt gttttcatac
    15301 atgaagcatg tccacgttgg accttgataa atcctttttg atcgatgatc cgaagacttc
    15361 cagcattttt tgaacgattc tgttcgccgc atacataaaa gaaaggtgta ttttccaata
    15421 agcaaacttc atttttattt aatcgcatat cctctgcagc aagctctagt gcttcttctg
    15481 ttgtgatctc cttggttaga tcggttgctt tcacttccgt agaaccagta gcgatagccg
    15541 ttacttttga agtctgtgga tcgatttcta cgtggacttc gatcgattct ggtgtagcgc
    15601 ctgattcaat cgctttattc atcgcttcat ttttcaacga tcgtatatct tctttacttg
    15661 gcgaagggat gatacgttct acaacatctc ggaccattgc aagtgctaca ccaatagaag
    15721 aaatcacttc ggcgttttca ggaatcgaat atttcacacc cattttatta ctaaagtaag
    15781 taatcaatga ggcagcgccg ccccctacac ctacaagaga aatctgatct ttctccaagt
    15841 gatacttgtc tgccaattct aaaatcaccg gttcaatctt agcataggat ttttccatga
    15901 tctgttctgc gatgtcttca accgtcgtat gacaataatc agctaatgct tggatggctt
    15961 ttcgtgcaga tgggacattt ccataagaaa aatgttcttc ttgcactaat ccaaggacat
    16021 tggctgcaca tgtatttgta atagtcactt cttctccgtc ttccatcact accttgacgt
    16081 aatcagcagg gtctccttct ttaggagaga aaaattcaac cttaggacct ttgatttttt
    16141 ctgtctccgt aaatactgca taatctagac cggcaatatg tgcagaacgt ggaccgacat
    16201 cgatgattcc tgactgatta gcccgtacca tcgaaccgcc tgccacacct agaacccgaa
    16261 catctaatga agagatatat gttgggtggc cacctacaat tgaataatcg attgctggac
    16321 gtccattttt aatgaccccg atattggttg ttgttccgcc tacttcaaaa taaacaccat
    16381 tagacgctct caagtacatc aaagagccca tgactgatgc tgctggaccg gaaagcatgg
    16441 ttaaaacagg tcgttttttc atttcattga tttccattac tccgccgtct ccgcgcataa
    16501 tcatcaaaga aacattgacc cctgcttctc gtacggaatc ttctgtagag gttgccgtat
    16561 ctagcatttt tggtaaaata gatgcattga ttgctgctgt ccgagttctt cttgtcaatc
    16621 catataattt tgtgatgtca gaagccatcg ttgtagggat cgattttacc gatgctgctt
    16681 cataaacaac tcgttctggt tccccattat ctactccaaa tgccatagaa gaaaccaaga
    16741 cctccgctct ttctctttct aaagaagaaa tcgtttctga aacacggtct acatttaagt
    16801 gtttgacagg taagaatgca ttgacaatct ctattttttt cttattccct aaatcgatat
    16861 cgttcaatct agtttgtcgt tttgctaaaa aaccttctaa tccacctttt gccatcccga
    16921 ttacgccgac ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat
    16981 gtgcaacaaa cacaacatct tctggactga tattattttc cagcaaacaa ttttgaaaag
    17041 actgaacgac acctgcggct actccacgga catcatcgtg ggttgttttc actgaagatt
    17101 ttccgatgat ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa
    17161 catcaattcc cattcgcaca aaacgtttca ttttcttttc ctccattcat ctaatcgttt
    17221 ttatcgacgt tttgtttact aggcgttccc gtacaataag aacgtgatca ggcataagat
    17281 cgctccgatg atccaaccag ttgggatgga tttcttcata tattctcgag aagttacttt
    17341 tgtataacct aatccccaag caacccaaga ttgtgtaata tctaaatgct gaggagcaat
    17401 cgttgtgatt gcaaataacg ggaacaaaaa ggctggcgac atcgttggat taacagctaa
    17461 tacgacagct aaaatagctg agcctgaacc gactagattc attggaccgc ggaaaaatcc
    17521 tagtggtgtc aatatagcaa atacgattgc caagactaat ggtgattgcg gtatccaatc
    17581 tccaaaaata gctgaaaaat aaggagaagc atacgcagct gaattattga acatcgctaa
    17641 ggtcatcaag aaaccaacca ttggcgcaac atctactgcg ccatctgtaa ataatttcgc
    17701 aaactgacga cagatttctt tataacctcc gcgcaatttt ccagtaaaca gcaatgccca
    17761 gatacctgct agaataaaac ctaagatgat tggaagatct aaaagaacga cccctaaaac
    17821 tggtaataat acggctaacc atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt
    17881 tgctgccatt gcatagcgtt tctttttgtt cattgatata ttagcgaccg ttagtaccac
    17941 aactaagctt acaatcatcc caatcatacc aatttggaaa tagtcattat aagtatagct
    18001 ttcagccgct ggattgaaac cagcataaat cgtttgatat tgtttgaaat tgacgatatt
    18061 agcaaagatt ccagccataa tcgagcccat aaatgagaac agtgcaaccg gtactgaaat
    18121 acctaatgac atcaagattg gcaacacaat aactgcaata gaaatcactg gtccgattcc
    18181 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
    18241 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
    18301 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
    18361 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
    18421 cgggtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
    18481 ggcaaagccg accatcaagt tgtagccttt tacacagtac cagaccatga aaaagaatga
    18541 gataactaac aaaataccaa taataatatc actcatgtta ttcctccgtc cattttgttt
    18601 ttctgataag caaactaata tatcagtcaa actcattcta acttatgtag ttagcgcttt
    18661 cattaaaggg tgaccataaa tgttcagttt tcaattaaaa aaccttaatt atgctatttt
    18721 agacttatac gttattgtcc tgtttcattt gatggaggat tgtaaataaa aaaagaggaa
    18781 agaacataac ttttcgttat cttctttcct ctttataatt gtttagctta tttagccaaa
    18841 gcttcttttg cttggtcagc caatgcagta aatgctgctg catcgttaac agccaagtca
    18901 gctaacattt tacggttgat gtcgatttca gcaactttca aaccgtgcat taattttgag
    18961 tagctcaagc cattcatacg agctgctgcg ttgatacgag caatccacaa tttacggaag
    19021 tcgcgtttct tttgacgacg atcgcgatat gcgtagttgt atgaattcat tacttgttct
    19081 tttgctgttt taaataaagt gtgttttgaa ccgtagtaac cttttgctaa ttttagcact
    19141 cttttacgac gtttacgagt tactgttcca cctttaacac gtgccatgta taattcctcc
    19201 taataaaaat aaatcttcag attctgttat ttgtacttct tatttcattt ttgctagttg
    19261 ttggcgaata cgtttgaagt cgccactaga aaccatgctt gctttacgca attgacggcg
    19321 ttgttttttt gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc
    19381 gcctttacca gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt gttttggcat
    19441 gactatattt cctcctcaaa atctttcgtt atccgactat cggctgtcca acagccagtt
    19501 agtcattcaa attacttact gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg
    19561 tccattttcg ctttttgttc cactgttgca atatccgcag tttcttcagc taagcgattc
    19621 aagacttttt gaccaatttc tttatgggta atggcacggc ctttgaaacg gatagaagct
    19681 ttcactttat cgcccttttc caagaactta cgtgcattac gaagttttgt attgaagtca
    19741 ttcaagtcga tcgtaggact taagcgaact tcttttacat tgatcacttt ttgtttttta
    19801 cgcgcttcgc ggtctttctt ttgttgttcg aaacggtatt taccgtagtc catgattcgc
    19861 gcaactggtg gtttcgcacc aggggcaact aacacaagat ccaagtttgc ttgttctgca
    19921 atattcaatg cttctgcttt tgtcttaact cctaattgct caccatctga accgatcaat
    19981 cgtaactctc gtgcacgaat gccgtcgtta accatcatat cctttgctat ggtcattcac
    20041 ctccaaatat tatgagagaa agaaaagctt accgaataaa cggcaataaa aaaacgagtt
    20101 ctcttacaga acccgcgcgt acttcaccta atagaaccag tgaaaactat ctagcccagt
    20161 gacattatca actaaggcga gaagcgggtg cttctgcttt tatttctcaa ccatcatagt
    20221 atagcaaaaa ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa
    20281 atgattagat tagcagactt tcttgcagtg attcccgctt ctttgctata ctaaagaaaa
    20341 acgaggtgaa cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca
    20401 aagggagagt tttatttaga agatgaggag atcttctctg gtcttctgat caacgggggt
    20461 gaccaaagct atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt
    20521 acgatgcaaa aaacagtttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt
    20581 gatttttcca atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt
    20641 aaattaacag gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt
    20701 attgcttcga tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt
    20761 caattagaag acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat
    20821 cagttgaccg gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat
    20881 ttcttcacag caatcgcttt atctcaagat tctttggcgg gactaatcgt caaccaagag
    20941 caagcacttg tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc
    21001 gccgccacag tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattc
    21061 cccatccgaa aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca
    21121 caatagttgg aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca
    21181 agttgttatt cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta
    21241 agttcgtcat ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt
    21301 ccttttctga caacttgcga taaatagcca atgccttgat ttgttctaaa agtactacat
    21361 tttgttttgt tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca
    21421 gattcttgtt cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca
    21481 cttcctcgat taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac
    21541 tgtatacact catgtctggc attcgttcca aaagcgatcg aattagtcga atcacgtact
    21601 cggtattcgg cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac
    21661 tagtttcata atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg
    21721 cttcatcaaa agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca
    21781 gcatctcgtc actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt
    21841 gctgtacatt gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct
    21901 atcctcctga catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct
    21961 ttatttgagc aactgtaaat caaatggcta ctttggtttg gcgaatctct gctaattttt
    22021 cttcgatcat tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt
    22081 ctccatcgat ttggattttt ggactttctt tgtattcttc ataccattga tcgtaacgtc
    22141 tgttcaattc tttgtaataa tcgtatagac ttggatcaaa ggacaattgt tcatacgaac
    22201 gaccacgttt ttcgatacgc tccaacatcg tatcaaaaga tacacgaata tgaactaata
    22261 aatccggatg ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt
    22321 catcatacac acgaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa
    22381 gcagtgaatc ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt
    22441 tgatgcttgc aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag
    22501 ggtttgcata aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac
    22561 tatcaaagtg ttcagccatc atttctgtta aacttgattt tccggcacca attgtacctg
    22621 ctaaaacgat cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact
    22681 ccaacctttt taaatatacc cccaatatct agtagtgtca attgtatttt tttctagtca
    22741 aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat
    22801 tcttagtttt acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc
    22861 ttagtggaca aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct
    22921 taaaaatcac ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc
    22981 aagcggaaag atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc
    23041 ctttcctttc tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga
    23101 tcaactggtt tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa
    23161 tatcaaaaaa ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact
    23221 gaccattgaa agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac
    23281 agcagtcact cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt
    23341 agaaaatgtc cagctttcgg aaaaaatcgc ccatggtaat cctagtggaa tcgatgcagc
    23401 cgctactagc agcttgcagc cgatttattt tacaaaaggg catcctttcg actacttttc
    23461 tttgaacatc gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga
    23521 agccgtcaaa gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat
    23581 ccaaaaatta ggatacttga cgaagcaagc aaaacaagca atcattgaaa attcaccaga
    23641 aacgttggca cagacaatgg atgaatcaca atcacttctg gaaaagctga caatcagcaa
    23701 tgattttctt aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt
    23761 aactggcggt ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca
    23821 agaaatcagt caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg
    23881 agtacatacc tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat
    23941 attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat
    24001 tggatgcatt ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat
    24061 tttatctaga tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg
    24121 acctttttcg taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg
    24181 ttcctaccgc agctggatta gcctcctctg ccagcgggct agctgcttta gcaggcgctt
    24241 gcaatactgc tcttaagctt ggattagatg atctctctct ttcaagattt gctcgacgcg
    24301 ggtctggttc agcttgccga agtattttcg gtggtttcgt cgaatgggaa aaaggccatg
    24361 acgacttaag ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa
    24421 tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc
    24481 ggacagtcga aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat
    24541 atcaattgaa acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa
    24601 gaaacggact aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt
    24661 ctcctgacag cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa ggcattccat
    24721 gctactttac gatggatgct ggccctaatg tcaaagtttt agtggaaaac agccatttat
    24781 ctgaggtaca agaaacattt accaaattat ttagcaaaga acaagtaatt actgcacatg
    24841 ctggtccagg aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac
    24901 caggcaaact ctatattgcc ggagaatatg cagttgttga aacaggccat ccagcagtta
    24961 tcgctgcagt cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta
    25021 tccaatctgc acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag
    25081 ttttagatat tcgggaaaat ccttttcatt atatccttgc tgctattcgc ttgactgaaa
    25141 agtatgcaca agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt
    25201 tagacagttc aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa
    25261 cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc tcagttggag attttcaaga
    25321 ttgcagcact agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta
    25381 gctgttatgg tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag
    25441 aaactcagca ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc
    25501 cattgattgc tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta
    25561 cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg gcttatcagc
    25621 ttttcttaaa aaatagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata
    25681 atgtaacgtt gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg
    25741 caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac
    25801 agtatgaagg agccgcaaaa tcttctggtg caggtggggg cgattgcgga atcgtaattg
    25861 ttgaccagaa atctggcatt cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc
    25921 cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga
    25981 acatgtttca ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg
    26041 gatcatccat aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc
    26101 agttgggttt acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa
    26161 aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc
    26221 cactggttca gtcagcgctg ccttgaaagt cccttcttta gccgatactt atacgattat
    26281 gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga
    26341 aagagccaaa gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc
    26401 acctcaagag ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat
    26461 tcaagaaact caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat
    26521 gacaagagaa acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg
    26581 tcgaagcgga acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag
    26641 ttatttagct gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga
    26701 cacttctatg gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc
    26761 cctttgtctc ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa
    26821 tcacggcgta gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct
    26881 ttatactatg gtcggcgcaa cgaataccgc ggccctacac caacaatctc tgatcttttc
    26941 cggaccagtg aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg
    27001 cacagaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    27061 caaacggcgg aagtaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
    27121 aagcaatttt cgtgaattcc ttcttatttc ttggagttaa atgcttctgt cccagcctct
    27181 tttttcgaat aaatgatgtt taatcagctg tccttcggta ttcgttcaat ccgctgattt
    27241 cacaacctct ccactaaaca gtgtactaaa gctcttttaa ggttgttttc cgatataggc
    27301 aagtatgcct ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc
    27361 caagaagaca gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg
    27421 tccaacgata aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg
    27481 agcagtttgc ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg
    27541 accgtattca gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata
    27601 agcactgacc gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc
    27661 gccgcctttt tcgatcatat ccggaataac tgcttttgaa acgatgaatg gggcatttaa
    27721 atccacgtcg attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt
    27781 gatgatccct gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg
    27841 aaccatcgct tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat
    27901 gcctgcttct ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa
    27961 tacgattttt gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac
    28021 agcaccggtg actaatgcta cttttccatc taaacgaaac atatccatac taaattccat
    28081 cattttctct cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat
    28141 aggtgatatt ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg
    28201 aatggatcga ccagcttggc gaaataatgg cttgttcatt gtctacaact aaatgttttg
    28261 tttcatctgg tttgcccatc atgtggaaca cacgtgtatc ttcattggca taatcaaagt
    28321 agacataagc ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcgcttc
    28381 ctggttctaa aatcgtataa cccatttgta attgacagct ttcgcataca tttggatgga
    28441 tgtattggta gattttacgt tcattcagtg tcaggccttc accagtctcc attggtttta
    28501 cttggtcgat actgattttt acatttggat atttatgatg agctggaaca gaactaatat
    28561 agaatttagc tgggttttca gcatcttgag aagaaaatac aacatgtcgt gtttcttttc
    28621 cgatatagta tccgtcttgt tttttcattt cttctttttg cccatcgatc tcgatgaatc
    28681 ctggtccacc gatattgatc acacctagtt cacgacgctc taagaagtaa tcaacaccta
    28741 gttctttgtc taatgtgatt tctaattctt tttcagtagg tgtcactcca ccaaagatca
    28801 ttcgatcgtt gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc ttttctacta
    28861 aaaattcttt tctcaattgt tccgttgaat aatgacggat atcttctgga ctgtgtgtat
    28921 aacgtgtttc catttcttgc attttcttct actccttcta tcgaatgatt tttccagagc
    28981 cgcatgctaa aaattgtgcg acttcttctt gactaaattg gttgcagtcc ccatggatcg
    29041 tatgcttcaa ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga gacatattgg
    29101 ataaaatccc atggagcacg ccacctgcaa aagcatctcc gccacctact cgatccacga
    29161 ttggggtgat tctatggact tgggattcca catattcatg gttcatccac aatgtcccct
    29221 tcaattcatt ttcacttgct gaatgaacgg tacgcttggt cgaataaaac acctgaatat
    29281 tcgggaactt ttcctgcatt ttttgataat aataaattag aggttgttct tctgcttctg
    29341 gcgctttctc gatacctaac aaataaagag catccatttt tccagctgag cagatatcta
    29401 ctaaaggtaa aagctgatgg attacttcgc tggcttcttg ctgtgtccat aattttccac
    29461 gatagttgat atcgaagctg atcaggcagc cagctttttt tgcagcttct atcacctgtt
    29521 tcgtcatttt ttgccatttt ttagacaaag ctggggtgat tcctgaaaca tgaaatatat
    29581 ctattccttg gaataactcc tctagcgacc attccaaatg atccatttca gcaaaacttg
    29641 atccggcacg atcatagatc actgaggcgg cgcgttcgcc actaccagtt tccatataat
    29701 aagtacctag tcttgatcca ccagataata cctgtttcgt atctactcca taacattgca
    29761 agtgcttttt gactgctttt cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt
    29821 catgtccgta attagctaaa gaaattgcta cgtttgcttc ccctccgcca taatgggcag
    29881 cgaatcgttc acttgaatcg atacgggttc cctgctctgt ggacaatcgc aacatgattt
    29941 ctccgagtgt cactactttt cccataagca gctacctctt gatttctttc atttttgctg
    30001 catactgttg cgcgacttcc gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa
    30061 ggttcccacc tactccgact gttacgacac cagcatcgaa ccactctttc atattgtcca
    30121 gactcacacc gccagttggc atgatattga cttgaggcat cggcgccttg aaggcagaaa
    30181 tgatacttgg tccataagcg cttccaggaa atagtttgac gatatctacc ccgcttttca
    30241 gcgctgtttt gatctcggta atagtcatac atcctggtaa gtaaggaatt tgatacagat
    30301 tgcacatctc tgccgtttct tgatcaaagg aagggctaac aatatactct gcgccagcta
    30361 agatagccaa gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc actatttctg
    30421 gttggtcttt gtagaatgac aaaagttctt tgattacttg atctgcttga gggactgtaa
    30481 aagtcaactc aataccagtt agaccacctt tgatgattgc gtggcaagca ttcaaagctt
    30541 cttctttgct ttttccgcgt accaccgcaa tcactccggc gtttttcaat cgagataaaa
    30601 tatcaatttt tttcatcttc tccacctatt tcctatttag aaattttatt ttataacttg
    30661 gaaatctata cccatattat acggcagaaa agaaaaattg caaacgcttt tttcattatt
    30721 tttgaattct ttgctcgatt tcgctttttg tttgcttgag ggcactgatt atttgttctt
    30781 ttgtctcatc tgttaaacga tatttcggca tacttacact aaatgcacca atgatttgtt
    30841 cgtttttcac gattgaggct cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa
    30901 atgcgatgtg ggtttctttt accgttttga tttccttttt caaccgtaat gcattggtga
    30961 tcgtatattc tgtgtaaggg atcaacggat gcttttcaag ataatcagtg tactgctctt
    31021 cactgaattc tgctaatacg gctttgccca tcgcagaatt gtataatggt cgggtgattc
    31081 cgatttttga agacatgcgg atcgtttgat gttttggatc tagtttgttc acgtacaaga
    31141 tctcattgtt gtccaatacc cctaagtgga tagtctcatc gatcttttgc tgcagctgtt
    31201 ctaaaaacgg taaagtcaac tctaccaaat cgatttgttc aatgttttta ttcgcgtagc
    31261 gaataagctt tgcacctaaa cgatagtttt tctctgaatt tttatttacg tagccgatca
    31321 tcaacaaagt atctagtatt ttcaagactg tagaagaggt cattcccaca ccctttgcga
    31381 tcgtctgcaa agagacatct tggttttccg ctaaatagtc tagtatttta gaggctttga
    31441 tcaatacagt tccataaggc tttttatttt ccatataata ttctcctatt tctttataag
    31501 aaaaattata gcggaaatac catcaattta aaagagatat ccagatataa actaccattt
    31561 ctctttacag gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa ctttgatata
    31621 aagcaatgat ttcttctact gtcgtttctc ttggatttcc tggtgtgcat acatctcgca
    31681 aagcatcttc tgcaattgct ggaatatctg cttcttttac acctaattca gaaatggttt
    31741 ctgggattcc aaccgctttg ctcaaccgat cgatctcacc acaagccgct tctcttacat
    31801 cttctaaaga catttcagct gctcctttaa tccctagaac aagagcgatc tcacggtact
    31861 tttctccagt atattctttg ttatatttca tgacagttgg caaaagtgct gcacacgcta
    31921 cgccatgagg aatgttgtac catgcactta acgggtgagc catcccgtgt accagtccta
    31981 aaccgacatt tgagaagccc attcccgcaa tatactgtcc taatgccatt ttttctcgcc
    32041 cattttgatc gccatctaca gaagcttcca aagaatggcc gatgatttcg attgctttta
    32101 aatgcagcat atcagtcatt tcccaagcgc cttttgtgat aaacccttca atagcatgcg
    32161 tcatagcatc catacctgtt gatgcagcca attttttcgg cattcccatc atcatatcgc
    32221 tgtcgatgaa cgcgacgatt gggatatcat gaggatcaac acaaacaaat tttcggtgat
    32281 ttacttcatc tgtgatgacg tagttgatcg ttacttctgc tgctgttcca gaagtcgttg
    32341 gtatagctaa tataggtaaa catggatttt tcgtatcagc aacaccttct aaactaatga
    32401 cgtctgaaaa ctctggattc gtcaaaataa tcccaatggc ttttgccgta tcgattggcg
    32461 aacctccacc gattgcgatc aaacagtctg cctctgcctc tttaacaaaa gccactccat
    32521 cttttacatt ttggatcgtt ggattaggta cgatcccgtc gtataaagca taagcgattg
    32581 ctgctttatc tagaagttct gttacttttg ttgctatacc gtgctcgatc aatcctttgt
    32641 cagtaatgac agctgctttg ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa
    32701 tcgctccttt tccaaaatac gatgtttcat ttaaaatcat tcgattactc attttctatt
    32761 cctcctaatt tgtattattt ttcacaataa aaacgaaaga accccaagca accggttgcc
    32821 cagttgcttg ttgctacagt tcaaagactg gcatcaattc ccaactgact ggagaggcat
    32881 ctggatttgt ctccatgata tcttccatgt acttccacca tttttgattg atagatgtat
    32941 ttgccatttt ttcccataac ttttcatctt cgatcactaa gtaaccaaat aacaaacttg
    33001 tactcggatc aagatagatc gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca
    33061 tttctggcca aagttcatca tgtctttttt tgtattcttc ttgtttttct gggtaaactt
    33121 tcatgcaaaa agcttttttc accatgtcag acacctgctt tcgtatgaac ttcctccaaa
    33181 taacctgctt ttggagtgac accaaacgca tctgctaaat gccacaggtt ttcgtccgtg
    33241 attgtttgca agattggtcc ttgcgctttg acgtacgtat agacttctgc tgccttttct
    33301 gctgtctcga tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta gattccatgt
    33361 tgtggccata aaaccaatcg agtttctttc attttttcag ctgttgcttc cccaatctca
    33421 tttgttcccg gaaccatcca cggaatgatg gaaattcctt ctggaaaaac aactaagcat
    33481 tccgtacaca tttgccatag cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa
    33541 ctcatcgcta atagatgact agcatggcaa tgcataacga tccgattatc tgggtctact
    33601 tctaaacggg cgatatgact catgaaatga cttggcaact cgcttgtagg aaggcattcg
    33661 ttttccagcc cccacaaaag ttccagtgtt ttgccatctt cagcaactcg aacgattcca
    33721 agattctcgg caggttcgct cgctacattt ttaaaatatc ttcctgaacc agttacaata
    33781 aaatagcatc ctgctaaacg gctggcatca aaaatcatcg agatgcggcg gatcactttt
    33841 tcaggatcca agaacggtgt gatctcttct tcttttagta aataagaaat attcccgcca
    33901 tttctttcat cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca
    33961 taaggcgctt gtaaaatatc tatttttttc aatatagcca cactcctatt ttcttaattc
    34021 caacacgtct ttttgatatt cctggacttc ttccagccaa tccaaaccaa ctggaacttg
    34081 atttttgtaa cagaaataat tccatacatc ggcatacggg aaatctttca attcttccgt
    34141 ataagccatt cttgaagtaa agtcaaaagc cagctccatt tctttcaatc gctcaactgg
    34201 ttccagcata gctttcagca gtgctttttg tgtatttctc gtaccgatga cccaagcagc
    34261 gatacggttg atcgttgcat caaagaaatc cagaccgata tttgtttttt ccaacaattc
    34321 gttacggact aattctttag cgatttcctg caattcatca tccatcaaaa ccacatggtc
    34381 tgaatcccaa cgcactggac gggaaacatg caacatgatt cctttgctga ataaagctaa
    34441 tgaagataat ttattggaaa tcacttctgt tggatgaaaa tgtccagcat ctagacaaac
    34501 aagtttgtca cgagtcaacc cgtaacccat ataaaattca tgggaaccga ctgtataagc
    34561 ttcagcgcct aaaccaaata gtttgctttc tactgcatct tgtacatacc gttcatctaa
    34621 ctcttctgta aaaatttcat cgagtgattc cattaaacgt tcccgtgggg taaaacgatc
    34681 aatcggatta tctttcatcc catcaggtac ccaaaagtta ttgatacttg tttgcccagt
    34741 ttctttccca aagtactccg cgattttgcg gcttcttttc ccgtgttcga tccagtattc
    34801 acgaacttct ttatctggat gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa
    34861 gaaagtcgga ttgaagtcaa gaccgatttc ttcttttttt gcccactcta cccatttttc
    34921 aaaatgtttc ggctcaattt catttagatc gacagattct tctgtatcca aatagatagc
    34981 atgaaggttc aatttgtgtt tgcccggtat caacgaataa gccttctcca gatcttgtct
    35041 aagctcttca ggtgttcgtg cggctcctgg aaaattccct gtcgccatga ttccaccggt
    35101 caattcgcca tccggtgtca agaaaccttt gacatcatct ccttgccagc aatgcataga
    35161 aattttgatg tcctgtatct tttctagtac agcatctgta tctacaccga tagttgcata
    35221 tttttctttt gcttgttcgt aatttctttc aatcgtgttc atacgattac tcctttgatt
    35281 ttattcacgt accattccat cgtgttttgc ttattttcat ctgggtaaaa agtttcacat
    35341 ggaaacgact gtttgattac atttcttgct tgttctatat tcgaaaactg acctgtactg
    35401 agcatttgca tggcaacatt cccgattgct gtggcttcac taggtcctgc ttctacaatg
    35461 attccggcat aatctgctgt caattgattc agtagacgat tattcgatcc gccccctacg
    35521 atgtgcagct tttccaattt cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg
    35581 taacaaagag caagattatc atacacacaa cgagcgatag ctcccgatga gacagggact
    35641 ggctgctctg tttcaaaaca gtattgctga atctcttcta tcatatttgg gggattcaaa
    35701 aatcgagggt cattgatttc catcacttgc tgcagcggtt tttcttttgc agcaagctct
    35761 gccaattccg cataactgta ctcgtactct tgcattcggg caacttcctg gatcaaccac
    35821 atccccatga tgtttttcaa aaaacgcacc gtgttatggg caccccattc attcgtataa
    35881 ttttcagaaa aagcattgcg agaaacattg gcgatctttg tttcaatccc taaaagcgac
    35941 catgtcccac tgctgatata accccaacgt tctccttgac ctggcgttcc taatacagct
    36001 gacgccgtat catgacttgc tactgtgata aaggtcacat ctggcaaatc atattttgaa
    36061 aattgttctt tttgcagatc tcctagtatc gttcccggct ctgtcaatgg tggaaataat
    36121 tccttcgaga caccgatttt ttcaagaagt tctatttccc attcttttgt ccctgcattc
    36181 agcagctgca tagtagatgc attagttttc tccgtcacca tgttaccagt aaagacatac
    36241 cctaaataat caggtatcag taacaattta tgtgcttttg ccaaattttc tttttcttca
    36301 gcaaacaatt ggaacaaggt attgaaaggc tggatctgaa tccctgtctg ctcatacaac
    36361 ttatctagag aatagatttc agaaaactgt tctactgctc cattggtacg cttgtcccga
    36421 taagcaatcg gatcatttat ccgatcccct tgttcatcaa tcagacagta atcgactgcc
    36481 catgtatcga tccccgcaaa aaggcgatca atccctgtct gctttaattt ttcaaatcca
    36541 atcaaaatct ctttgataag ataatcgatc ttccagcgat cgtgaccgtc tttgttccga
    36601 aagccgtttt tgaaccggtg tacttcttcc aagatcaaag aaccttttgc atccctcgta
    36661 cttttcatta gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt tggattcatt
    36721 tctccacctc ttttacttta ttgttgatta gcactttatt aataaagcgt tatcatttat
    36781 tgacaatgct atcataatac agaatcttat attttgcttc acactatttt ggtatataat
    36841 tgcattatct tgacaagttg ggagtgttta catggattac tgggaaaaag ccaaccactc
    36901 atggtcggaa gactcaaaaa gagtgatctt gacgcctaca caaagaagca ggaatttatt
    36961 tttttatatc caagaaatcg gttacttcaa agcaccaatc ccttactata ctgaacgagc
    37021 acatttacct tcttacttga taaagtttac tttgtctggc aaaggttcgc ttacatataa
    37081 tgataatcag caacagctaa aagctggaga cttatttttt attgactgcc aagaatatca
    37141 gcgttatcaa acgatcagcg aagaaccatg ggaaatggat tggatccatt tgaatggtca
    37201 gaacgtccaa tcattttatc aggagttcac aaaagacggc cataacacct tccattcaac
    37261 aggagatcca tcagaaaaca agatccacca tttgatcaca gaattgatcc acatgcaaca
    37321 aggcacgaat gcccgtaccg atttccagtc ctctgtagct atccacgagt tgctgaatga
    37381 actgattttg caaaagtatc agctagattt cgaagaagag gatattcccg cctacgttta
    37441 caaactgaag caatacttag acgaacattt caaagaaacg attacacttg atcaattaga
    37501 acaacaattc ttattgaata aatatcagct gagcaaagat ttttccaaat ttatcggttc
    37561 tccaccaatc gattatttga tcagcaaaaa actttcttac gctaaagacc ttcttcggta
    37621 tacagaattt acggttcgaa tgatttccga agaaataggg attgagaatt ttgcttactt
    37681 tagccgcctg ttcaaagcaa aaacaggact atcgccgaga gactttcgta aattcagctg
    37741 attcatataa tatgtacgtc attttttcaa aatattttga aatatataga taaaaataaa
    37801 ataaccgcag tttctgcttc tatgtaaaca acagcagcta ctgcggttat tttttagtga
    37861 tcattctcat tgttcatctt caaaatgttc ataatgaacc aactgtttct gtgtatcacg
    37921 tttcaaaaat tctttaggcg ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg
    37981 ataagaagaa aaaccacttt tactatatac agcactagag ctgcggtatt ctcgcaacaa
    38041 gtgcttggca tagatcagct ttttcttcat tacatactga tgaaacgtaa atccagtgta
    38101 ctttttaaat tctcgtgtga tatggtattt gctgacaaaa aactgctttt ccatttcctc
    38161 taaagaaagc ggatgagata aattttttga gatatattgt aacatttgtt ccacacgctc
    38221 attaggaact acagcattct gttcttgcgc actctcttct ttttgtacaa cttgattgag
    38281 aaaaatcaag tattctacca gagcttgttc atattttatg tcttcaccgt aaattttttt
    38341 atcaggagga tggtcgataa atgataattt ttgcttcaat acttcctgtt ctatttgcaa
    38401 tacacgactc ctcttctctc ccattggttg aaaacaagcc gatagattag accatggcgt
    38461 ggaacgactt tctagaaaag aaggggtgac aaagatatag acacgctcga atacatctga
    38521 agattggcgg acgatacgat gcaaatcatt ggaatggatg agcagcacac ttcctgcttt
    38581 gatcgtaaat tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg tagcatggat
    38641 ctcatagaaa tcatgatgat gatagatcgt tttattttgg ctgacgacat ctttgacatg
    38701 atagatttca tactgatcag aaaccatcgt attgacttgt tcaccgctga ttttcttttg
    38761 gagcatgtgc cgcacctcct tttgttcact tatgcacaat tttattatac cacgtttttt
    38821 gtaatcaaaa agagctgctt ttatatactc ttataaaaaa gaagagacgt ggcatcttct
    38881 agccccgccc cctccattct aaatattagt taaccaacgt cttctttaca ttattcaaga
    38941 agacttcttc cataacgaat cccatcatct tttgtttctt caagaaccac atacaggtac
    39001 ggacgattct tcatgatgat ttctttgatt tttgtgtact cgatcacacc gtctcccaaa
    39061 tttaccggca cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc
    39121 ttttcaccaa aacgttcaaa agcttcttct accaattgga cttgttcttg ataatcacta
    39181 cttgtgatca aattggtcgg atctaaaatg attcctagat agtccgaatc tactgcttgg
    39241 atcagctgct ctacccttgc taaagaatac accggatgat tcaaacctgg ttctatcccc
    39301 accatcattt ggtatttttc tccagctttt accaagcgac ggataacgga cacagcttca
    39361 gcaaatgctt catctgtgaa attttcttcc gtatattgga tttccggcaa aacacatcca
    39421 gtttccgaag caaccatcga cgcaccaaag gaagaagcat agcgaagata ctgctcgaac
    39481 tttgtcagca actcttgtcg tatcgttaaa tctggatgaa tcatattgat atagcaactt
    39541 aaaattccta cggtcacttc ttgcttttct aaaactcgtt tgacataatt tcccatccca
    39601 gaattgatct catttgcgcc agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc
    39661 agtgtatgaa tcccttgttc ttttgttttt tctgcaagat cttctatcgt ttggacttcg
    39721 gaaagatcat gtcctctaat tgtaatgttc aacataacta ttctcctaac tcagcatatt
    39781 gggtagccac atgacgattt gcgggaagta agtaatcaaa aacaagacga ctaagataac
    39841 cccataaaaa ggaagaagcg gtttcagcat tgcctctgct tttacgcctc ctacacttgc
    39901 accgacatat agtcctgtac ctactggcgg cgtgatggag cctacacaca gattcatgat
    39961 tgaaaacaac ccgtaatgta caggatcaac ccctacactt gtgacgattg gtaaaaatat
    40021 tggtgtaaaa atcaagatag caggcgccac atccataaac atacccacca gtaataacac
    40081 aagattgact aataacagaa taataaattt attatccgtt acgcccataa tcaacgaact
    40141 aattgcctga ggaataccgg taaacgccat ggcgaaagac atcatggaag aagtagccaa
    40201 taagaacatg atcgtccctg acattgctac tgattgaaac aacatattag gtatatcttt
    40261 tagttttacc gttttatagt aaaacatagc aatcaacagg gagtaaatga cagctatcgc
    40321 tgaagcttct actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa
    40381 tagaaggcta ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc
    40441 gacaacaggg tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc
    40501 ccacaaagcg cctacgagat aacctcccat aaacaaagca gagatcgaag caccaccagc
    40561 aacaagtgaa tacatgataa aagcagtact tggaggaatg accatacctg tcggagctga
    40621 cgcgatattg accgctgtcg caaacttccg gtcatatcct tcttccactt gctgagggat
    40681 caacactccg ccaatcgcgg tagaagcggc aatggccgag ctagagatgg aaccaaataa
    40741 agcattccct aacacattcg tatgagccaa agcccccggc acgcgcccga cgaacaacat
    40801 cgcaaaatcc actagtttct tagctatccc tccattattc atgataatgc ccgagagaac
    40861 gaaaaacgga atagcgatca gcgaaaaact attgatactt cccaccattt tttgagctga
    40921 actaaaagca gaaacatcaa acggtataac taacaataaa gtcatcatcg atgagaatgc
    40981 aatacttata gcaatcggca tccctaatac aagcaacaca acaaatacaa aaaataacac
    41041 taatccggct tctattgcca ttttattcct ccatttttat attttttctt gaattgctgg
    41101 cttgttgctt aatgtatcga ttagattgat caggctatag ataatgatga gtacccccga
    41161 tacaggtact gacagataaa catatcccat aggaatactt aaagaaggcg agatctgccc
    41221 cattgtcaat gtgacagcat tccatccgcc atacaccatg atcatcgcag caaataccag
    41281 aaataaactt tgaacaacaa cttctaaaat tttacttttt ttgccagtta atttttcact
    41341 taaaaccgtt acggctaaat gtgctttttg tcccacaaca tacgcggaag acaacatggc
    41401 aaaccagaca agaccgaatc gaacaagctc ttccgtgacc gtatttggat ttcctaaaac
    41461 cgttctggaa aatacttgcc ataaaacaac agcgaccatc ccaacaagca aaacagctcc
    41521 caggatctct aataccttgt ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg
    41581 atcttttgat aaaggtcttt gtaggtagga tcgtttttga atatatcatg aagtggggca
    41641 actgcctctc gaaacggtgt attatctaca tcattgaatt ggactccatg ttcttcttgt
    41701 gctaaagctt tttcttcttc aattgctttt gcaaaagctt caatttcaaa aaccgttgat
    41761 tctttcgctg cttcttcaat ggctttttcc tgctcatcag ttagctgttc tttgatggca
    41821 tcattcatga tgatgatatc tggcacacgt gtatgctcat cataggaata gtatttagcc
    41881 acaccaccat gtccagctgt atacaagaca aattcattgt tttctgatcc gttgatcaaa
    41941 ttggattgaa gagacgtgta cacttcatca cttcccatag gtacgggcga accgcctaaa
    42001 agctctacca ttttgatagc ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa
    42061 tcttccggcg tatggatcgg accgtctacc atataaaagc tgcgctgacc agaatcataa
    42121 taggtcaatc cggtaaatcc taaatttttg gtcgattggt agattggctc cattatttct
    42181 tcattatcca tcaccttaaa gaaatgttct tcatcattga atagataagg aactgaaaaa
    42241 atcgaataat ctttagagaa actttccaac gctgaggcac ttacttttgt gaaatctact
    42301 gctcctgact gtgtcaattc gatcagttca cgttctccac ccagttgtcc atctgggaaa
    42361 tactggatct gtacggaacc atctgttttt tcttttacta aacgcccaaa ttcattcatt
    42421 gcatcaatta ctggctggct gttgcttgca taagcaaagc gcaaggtaac tatctcattt
    42481 acatctgcat ctgcgacagt tgccacacta gaattatttc cgcagccgct tagacctagc
    42541 agacctaggc taagcgaact tgctgcaaac agcagtgatg tttttttcat atgggtttcc
    42601 tttcttttaa caataagaga cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc
    42661 ataaggcatt gctgtgattc cgatattccg ataaaaatct aaactgtccc ccattcctgt
    42721 cccgattgaa acgttttgaa cttctccttt ttcatcgatt tgttcaagca atccttggat
    42781 agcacgataa gcgacttctt tatattcttg tggtaaataa cgtttatgga ccgctttcaa
    42841 aataccgtaa gcaaatcctg cagttgcaga agattctacg taagaagaag gatcatccaa
    42901 caacgtatgc cataaaccgc tttcagactg ataagttttt aatgcacgca cttgtgcttc
    42961 aagtgtttcg atcaataatt tgcgcaatgc atcttctttc gataattcta agatttcaat
    43021 gatctctgga atagcaatcg tcaaccagca gtttccacga gcccaaaatg cttcggcata
    43081 gttatgattt ccttcaaacg tccagccgtg ataccacaat cctgttcgtt tatccatcaa
    43141 gtatttgata tggatcataa actggtaacg agcttcttct aaatattctg ggcgattcaa
    43201 cagtttcccg atttttgcca aaggaagcac tgtcatcatc aatgtatcgt cccacaactg
    43261 attcttattt tccggtccat atgtggcatg ctgtaacccg tcttcatttg tccgaggcat
    43321 gtcattcatg acccattctg cccattgttc taaataaggc aaatagcgtg aatctttcgt
    43381 gtcttcatac aagtaagcca tagtcaatag cggtgccatt gtattgacgt ttttgggcgg
    43441 agcgccttct tccatacgat cctcaaacca ttcattgacc acttgataag cgcgaggatt
    43501 ttttgtcatc tggtaatttt tataaatacc atatagaccg acaccttgtg gccaattcca
    43561 cactgtccag cttttgtcat ctactttcag tccatcaaag tccaacaaaa attcaccatt
    43621 atcgtctttg atcgttgtca ggttttcaac gagcaggtca attttttctt tcactaattc
    43681 tttaggtaca aaaatctctt ctttaataat catctttacg ccaccttttt aaacgctttc
    43741 attttatatc cttaatatag cagtaatacc gtccactata aatatcgctt tttgaacaaa
    43801 aactgtctaa aattgaccta aatgttaaat aacaaaagta actttcagtt acttggaaat
    43861 taaaaaaaga acttggagaa acaacgtttt ccaagttctt tttcatctat tttcttatct
    43921 tttttctacg ctttcttttg cttatccgtt gtaagcatca atgatgatct gtttcaattc
    43981 agagatcaat ggttctttcg gattagctgt cgtacattgg tcttcgtaag ctaattcagc
    44041 catacggtct acggttgtat ctagtgtttc ttgtgtgact ccttgagctt ttagactcat
    44101 gttgatgccg acagattttc ctaattcata aacagccgtt gctaaagctt cgactaattc
    44161 ctctgttgtt tcgcctttca gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc
    44221 acggaaatag tcatatttag ggaacatcgc atgtttttgt ggatcttttg cattatagcg
    44281 gataatatgc ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt attccccgcc
    44341 gattttatgt gcgactgagt gacaaatacc taggaatgcg ttagcaaacg ccataccagc
    44401 cattgtagaa gcgttgtgca ttttttcacg tgattccata tcaggtgttt ttactgattt
    44461 ttctaaatag tcaaagacta acttgattgc ttgtaggctc aatccacgtg tataatctga
    44521 agccatgact gaaacatagg attcgattgc atgtgttaac acatccatcc cggtatctgc
    44581 tgtgacagat gctggaactg acatgacgaa ttgaggatcg acgattgcga catctggcgt
    44641 caatgcataa tctgctaacg gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa
    44701 tggcgttact tcagaaccag tccctgatgt tgtcgggata catacgaatt gtgttttttc
    44761 tggtttgtcg atcttatatg tccgtttacg gatatccaag aatttttgtt ttgcaccaaa
    44821 gaaagatgtg tctgggtgtt catagaacat ccacattccc ttagctgcgt ccattgcaga
    44881 accgcctcca agagcgatta ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc
    44941 gtatacggta tttgtcgatg ggttcggttc aacatctgag aagacttcga tcttcacatc
    45001 atttttacgt cgtgccaata cttcacgaac tgtatcgcag taaccgaatt ggaccatacc
    45061 agggtcacaa acgatcatga cacgttcaac gttttccatt ttctctaagt acaatagaga
    45121 gttcttttca aagaagattt ttggcggtaa tttgaaccat tgcatgttat ttctccgttt
    45181 cgctacagtt ttgacattga ttaagtttac agcagagacg ttacgagaaa cagagttctt
    45241 accataagaa ccgcatccca aagtcaatga cggaatcatt tcgttataga tatcaccgat
    45301 gccgccttct gcagatggtg tattcactaa aatacgacaa gctttcattc gaagaccgaa
    45361 tttgacatgc aactcttcgt tttctgaatg gataactgct gtatgtccta atcctccaag
    45421 attcaacata ccttcacaca aatcaaacgc atggtccgtg ttgttagctt tgatcatggc
    45481 aagaactgga gaaagttttt ctcttgatag cggataatct gggcctacgc cttccaattc
    45541 agcaatcagc attttcgttc cttttggtac actgattcct gccaattttg cgatttccat
    45601 cgcagaatgt ccaacgattg atggattgac cgcatatttt ccttcgttca tcactgcatc
    45661 ttctaatttt tgcaattcat ctggttttac gatataaact tggtgtgctt ggaattctgc
    45721 ttttactgct gcatagattt ctttatccac gatcacagct tgttcagaag cacaaatcat
    45781 tccattatca aatgttttcg aaacgatcaa atcgttcact gcacgtttaa tttttgctgt
    45841 tttttcaata taagctggta cgttacctgg acctacacct aaagctggtt ttcctgtaga
    45901 gtaagcagac ttgaccattc cagctccacc tgtcgctaag acgatcgcga tacctggatg
    45961 gttcattagc atagaagtcg cttcgatcga aggatgttcg atccattgga tacaattttc
    46021 aggagctccg gcagcgatag cagcatcacg taccactcgt gctgcttctg cagaagattt
    46081 ttgggcactt ggatggaaag caaaaacgat tggatttctt gttttcaacg cgatcattga
    46141 tttgaaaatc gttgtggaag tcggattcgt tgttggagtt actccgcaaa ccacaccgac
    46201 tggttcagca atttcaatca accctttttg gacatcttca ttgatcacac caactgtttt
    46261 atcatgtttg atattatgcc aaatatattc ggacgcatac atatttttga ttgctttatc
    46321 ttcgtaaaca ccgcggcctg tttcctctac agccatcttc gctaatggca tatgttgatc
    46381 caaagctgcc attgccattt catgcacgat atggtctact ttttcttgat caaagctttc
    46441 catttctttc aatgctgcat ttgctttttc tgccaaatca tcaatcattg cttgtacgtc
    46501 aattgtcttc gttttttctt tttctattac tttagccatc attatcctcc taatgggtca
    46561 tggttatttg ttaaataatt cacatgtata tcatacaata tttatttttc tatgtaaaca
    46621 cttttttgtg atttttttca cagttatttt taaatataaa attgctttat ttacaggtgt
    46681 ttgtatagaa aaatatataa agttatttat tattatttat atgacacacc cgattattca
    46741 atattgagca atatatccca tgctcttatc tctttctttt ttgaataaaa aatgaggtag
    46801 gacatctttc tgccctacct cgtctttaaa taagctttga tacttatttt tcttccttct
    46861 cttctgtctc ttccagcgtt tctgttgttt cagtttttac ttcaactgtc tcagctggtt
    46921 cttctgttgt ttcaacagta gtcgttgcat catttgttac gacagtccct ggtttgaccg
    46981 ttttgattgc tgcacgatcg tactcaagga agatcccttc gcagtctaaa acaactgtcc
    47041 gtttgtcatt gtcgatttct gaaacaactc catgtaatcc gccgatcgtt acgactgaat
    47101 cacctggttt catgctatct aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac
    47161 gtgacataaa aaaccacatt gctaatagcg cgataaacat cacaagcatt ggtaatgttc
    47221 ccattttttt cacctcacta attctgtatc tacaaaaaac actttagcag aaaaatgctg
    47281 ataacactag ttttttactt aatttttaaa agattttcct ttagaagctt tttgcgtttt
    47341 ctttattgaa cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca
    47401 tgattgcttg acgtacttgt ttcattagat tcaacaagaa gtataaattg tggtaagaag
    47461 tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg gcggatatag gcacgtgtat
    47521 agtttttaca tgtataacaa tcacatttct catcaattgg acggaaatca tgggcatatt
    47581 tcgcattttt cactactaga cggccttgag aagtcataca tgtgccattt cgagcgattc
    47641 gagtaggcaa gacacagtca aacatgtcta ctccacggat aacaccatcg atcaatgaat
    47701 cagcagcacc gacacccatc aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga
    47761 attctaatac acgattcatt tctgatttag gttccccgac agataacccg ccgattgagt
    47821 aaccagggaa atccatcccg actagatctt tcgcgctttg acgacgaaga tcttcaaacc
    47881 ctgctccttg gatgattcca aataatcctt gacggtctgg attagcatga gctttcaatc
    47941 ctcgttctgc ccatcgagac gtacgttcta ctgatttttt cacgtagtcg taactttcat
    48001 cgaatggtgg acattcgtca aaactcatca tgatatcaga gcccaattta ttttggatgt
    48061 tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca
    48121 cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat
    48181 ctgttaaaat cggttgatcc cagttcataa atttatgcag tccgccagct tcttctacta
    48241 agtcttcacc agggcgtagc cataaatgat atgtattact taaaataacg ccagcaccca
    48301 tttctttcaa ctcttctggg gacattgttt ttactgtagc taatgttcct actggcatga
    48361 acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat
    48421 gtttttcttt cttgattaaa cgatagcgaa tggcaggttg cgtcattcat ttccctactt
    48481 tctatataaa atatcttgac aactcccacc atcataatcg tttcaagtgc tttttgcaag
    48541 ttccctcggc ttccttctac tttatttaat attctttttt ttatcctttt tttatgaata
    48601 acaaaaaaaa gaaaaacatt atgacagaaa tattttttga tacatggtat attggtgtta
    48661 ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga attaaaacgc caagcaaaag
    48721 agagtctaaa aggacgttgg ggacaagctg ttttactaaa tctaatccct acattgattg
    48781 gtattgctac tattcttctt attatgattc ctattatctt gtttgccacc accatatatg
    48841 atggatcagc gagcgtctca gaagcgacaa cttcaggagg cggtagttca ggaggcggtg
    48901 gcggaattat ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc
    48961 ttgatatttt acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat
    49021 tcaaaggttt gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa
    49081 cattatgggg tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa
    49141 gctattttat ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata
    49201 cgatcactgc aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc
    49261 tttcatttat cggctggcat atcttagcat tagcaactgc tggtattggt tatctatggc
    49321 tcagcccata catttctgca acaaaagcag cattttatga acaattgcca aaagatattt
    49381 aaaagagagt gtgacttgtg accgaggggg tggcaggaga acaccgttta tcgagaggca
    49441 gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg acaacattca tgtcacacct
    49501 cctttttgta tagttccagt aaaaagaaaa tcaacttaga aacagttaat cattcctctt
    49561 cttctgcttc cggtaatcga tggaacttga tcagttcatt caccgcgatt ggtacgatag
    49621 ataaaatccc taccaagaac cagtgattca aactgatgtg tactaggccg aatagttctt
    49681 gcgtaaatgg cagcaaagca acagccgttg taatagctaa tgccagtaac gccatttcta
    49741 ccaaagcttt attggtcgct aaattgactt tgaagatcga ttggctgctg cgtacattga
    49801 acacatgcag aatcgatgaa taagctaaga tcaagaatgc aacagtttga ccgacatgct
    49861 gacttgcgtc tacccatggg ctgatagtat cgacataact acctaaataa taaccaaaca
    49921 aggttacgat ggtgaagacc cctgcattta tggcaatttt ttgcaataaa cctcgagcaa
    49981 agatcccttc atttttcgga atgggtggct gatccatgat gccgtgttcc gctggctctc
    50041 ttcctaaagc aaatcctgga agtccatcag caactacatt gacaaacagt agttgcacag
    50101 ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa aacaataaag atctccgaaa
    50161 tattacagct tagcaaaaag ttgatagctt tacgaatatt ctgataaacc gaacgtcctt
    50221 gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac
    50281 tttgcgccac atctgttcct gtgatcccca tggcacaccc aacgttactt gctttcaacg
    50341 ctggtgcatc attcacacca tccccagtca tcgctacgac tgcacccgta cgttgccaag
    50401 atttgacgat ccggatcttg tcttctggcg ttacccgagc atagacagag agagctttga
    50461 cacgcgcatc taattcttcg tctgataatt gatgcagttc tgatcctgaa agtgcttctg
    50521 acggatcatt caagatacct aattctttcg ctatggcact ggcagtcacc acatgatctc
    50581 cagtgatcat gactgttttg atgccagcct ttttcgctcg cttgattgct cctttacttt
    50641 ctggacgtgg tggatcgatc attccgatca gaccaatcaa acgtaagtct ttttccagtg
    50701 cttcagaaga aatatcttgc ggttcttcgt cataagtagc ataaccaaca gcaatcactc
    50761 tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac
    50821 taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat tgagatgtat ttttcgccca
    50881 tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg tttactcgtg
    50941 gatatttttc ttctaattct gcctttgtat gataattctc ttcaacagcc cttacgatgg
    51001 ctacttctgt tggattacct ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat
    51061 ctgtacatag tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga
    51121 tattcgttac ttcatcttct ttgctccata cccggcggac gcgcatttca ttttgtgtca
    51181 gcgttcctgt tttgtctgag cagataacat tagcggttcc tagcgtttca actgctggca
    51241 actgtcggat gatcgcatgt ttcctcgcca tcttctgcac accgtatgca agtgtcaacg
    51301 taacaataac agtcaaggtt tccggtacag ctgcaacagc caaggaaatc gaagtcatga
    51361 acatctcaag gagtggttct ccttgcagtt caccaatcac aaatactaaa gccgcagccg
    51421 ctaaagcgat catactgatt cgtttgccta gctgattcaa tcggacctgt aaaggtgttt
    51481 tttgcatagt attttcgttc aaaagatccg cgatcttacc catttctgta gccatgccaa
    51541 tggcagtgac gatcgctttc ccacgacctg cagcaaccgt acatccttta aataccatat
    51601 taagttggtc acctaatgac tcatcttctt cagcaataaa tgctgcatct ttttcaactg
    51661 cttcactttc tccagtcaac gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac
    51721 gggcatcggc agaaatagca tcaccggact ctaaaacaag aatatcaccc actaccagtt
    51781 gctctgactc aacagtggtc actttgccgt ctcgtatgac agttgccgtt tgtttattca
    51841 tatcttgcaa ggattccagt gctttttctg cgtttccttc ttggacaatc gccaataccg
    51901 agttgatcac aacgatagcg atgatcagca gtccttcaaa taaatcccca tgttctgtag
    51961 caaatgcggt ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg
    52021 acaagcttcg aaagaatttt ttgatcatcg attctttcgg cgcctcatcg aatttattca
    52081 atccctgctt ttttcgccgt tcatccactt cttgtgaaga caacccagta tctggatctg
    52141 cttctgttac cttctttact tcttctattg tctgcttgta ttcttccatc ctattcctcc
    52201 tttaaaatct tcgtacaaaa aaaggtactt attttactat aacatccttt ttttatttac
    52261 ttcaaataaa gggcttatcg attatttgtt aagaaagcgt aaaatttaaa gtgcttatat
    52321 ttgcaaacgc taccaaagga tgctattata tagatgtaag gaaggaggat accatcaaat
    52381 agacagaaga atttctcgat agaacagtgt aagcaaacaa agttgttaga agtacaggtt
    52441 tcacccagac atgcgtgtag agtacaaacc tataaatagt gaatgcgtat ggatgtttgg
    52501 atcacgaagt atccgaaaca agaagtaatg cgatacgtaa caattgaata acgtaaacgt
    52561 ctgaaaattg aataggaaca agagatgttt caaatgttga agatgtaacg aaaacaaaat
    52621 ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat gtatgatgga aagttacaat
    52681 catttctgtt tatttgaatc cttccgaggt acaagaaagc cgagcttatc gagagctcgg
    52741 ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt
    52801 cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa
    52861 tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga
    52921 ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat
    52981 agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt
    53041 tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc
    53101 catttttacg tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac
    53161 tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta
    53221 catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt
    53281 tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag
    53341 tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc
    53401 ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc
    53461 cgtgttccaa ttcttctttc actactgctt gcaatctgcc atcaccaaaa gagattcttg
    53521 tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat
    53581 tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag
    53641 caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga
    53701 tttcatggaa atgtttatct tccatctctc cagttttccg atccaaaaca agtaaacgag
    53761 aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa
    53821 aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca
    53881 tgtttttcgt tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa
    53941 taaaaaaact accctatttg tattttttca ttgttttagg catactagta atagatagtt
    54001 cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat
    54061 ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt
    54121 catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg
    54181 aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga
    54241 tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca
    54301 catgacgatc cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga
    54361 acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga
    54421 tcgctaccca catgaattat ctggtggtca gcagcagcgt attggagtcg ttcgtgccct
    54481 tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg atccaatcac
    54541 tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt
    54601 atttgtaacc catgacatgg atgaagcgat taaactagca agccgtatcg ccatcatgag
    54661 tgaaggccgt gtgatcaatt tgatacacca gaaaatattt tacgtcatcc tgcaaacgat
    54721 ttcgtagaag agttgattgg agaagatcgc ctactacaag ccaagccaaa tgcgacaaga
    54781 gtcggcgaag ttatgctaaa tacagcaatc acgattacac cggaaaaatc attacaagaa
    54841 gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt tagttaccga taattcgaat
    54901 gtattaaaag gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt
    54961 gtcggcgaca ttttgaataa acatgttttt tacgtaaaag aatccgccta tcttcgtgat
    55021 accttgcaac gcattttaaa acgcgggctg aaatacgtgc cggtcgttga tgaacagaac
    55081 aaagtcgtag gtatcctgac tcgtgcatca ttagtggata tcgtctacga tgtgatttgg
    55141 ggagaagatc ccgcaactac tgatgttgca gagtcagcta ctcctgaaga atctaaggag
    55201 gtctgaccta tgcagcagtt ttttcatcag cacggttcag aaattctctc aaaaggtttt
    55261 gaacatcttt atatttcagg acttgctttg atcattggta tcatcattgc tgtaccgctt
    55321 ggtgtcttac tgacacgttt tccaaaagct gccacgattg tgattggctt aaccagtgcc
    55381 ttgcagacaa tcccttctct tgctttattg gcattgatga tcccgctatt tggcgtaggt
    55441 aaattacctg cgattatcgc actctttatt tattcattat tgccgatttt acgtaatacg
    55501 tacattggga tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg catggggatg
    55561 actgagttcc aatccatttt ttccgttgaa ttgcccattg ctatgccaac gatcatggct
    55621 ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc
    55681 gctggtggtt taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt
    55741 ttggcaggta cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc
    55801 ttggaacacc ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac
    55861 taaaaatgac catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc
    55921 ttgccagcaa tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac
    55981 agatcttagc aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa
    56041 tcatcaacaa tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt
    56101 caatttcagc ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg
    56161 aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc
    56221 aaacgtggtt cccgtcctat agttttgaaa atacgtatgt ctttcttgtt cgaaaagaca
    56281 ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag
    56341 tagctggtgt agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag
    56401 aaacttatgg cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg
    56461 cagtcgaagc aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca
    56521 gttatgatct agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc
    56581 cagttgtaga caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt
    56641 taggtcatac catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc
    56701 tagtggaacc atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga
    56761 agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa
    56821 tggctcctat atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct
    56881 ctttgcagca gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc
    56941 aaactgggtg attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc
    57001 cattttaatc attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta
    57061 ttccctctta ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat
    57121 ccttgatgtc ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt
    57181 accactctct gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg
    57241 gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg
    57301 aaccaatgca acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat
    57361 ggcgatcatc acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg
    57421 tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa
    57481 aggctttttt agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat
    57541 gggcaaagag ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc
    57601 caacgggttc atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg
    57661 ccaatccatt ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt
    57721 ctgcttgttc tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag
    57781 ccttttccat cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact
    57841 gccgtatcgg taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg
    57901 gctaaaaaca tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac
    57961 cggtacacga ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct
    58021 tcaccagaag agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat
    58081 tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg
    58141 aaagcgccac gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt
    58201 tggatacccg ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc
    58261 caaaagacca tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca
    58321 ttgctttttt caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg
    58381 cctcactttg gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa
    58441 ttttactgat tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt
    58501 ccattcctaa actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa
    58561 atcatgcaat aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg
    58621 agactctttg ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca
    58681 ggcagaataa atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg
    58741 tctattcagc ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg
    58801 cgtgggcata tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa
    58861 attcgtttgc ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata
    58921 gttactcatc aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct
    58981 ttttcgcggt ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga
    59041 atcgctctcc agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata
    59101 atctcgattt cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga
    59161 gcagtgctag gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt
    59221 atagcgtact tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact
    59281 tttcctcttt tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc
    59341 acttcttact cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc
    59401 atctttagca aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa
    59461 ggttcctcgt tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa
    59521 gatttgtaac ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat
    59581 gattgcctct ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc
    59641 gttgggtttg tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca
    59701 cttgacgagg ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag
    59761 gaagaagctg aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat
    59821 caagacatca aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg
    59881 ctgttcccat ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga
    59941 ccctctagct tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt
    60001 cttcaaatgg tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct
    60061 taaccgcaaa cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg
    60121 aggaatcaac acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac
    60181 ataacggata attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac
    60241 atctgggaaa tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc
    60301 tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc
    60361 gatatttacc cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg
    60421 tagttttgca tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat
    60481 cttataaaaa atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa
    60541 ccgatagaca aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc
    60601 aatcgatttg cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg
    60661 agataaagca aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga
    60721 gccatggaaa ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga
    60781 atagcctagt cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag
    60841 attgtatgca agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag
    60901 cgctgtagca aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat
    60961 ttgagacaaa acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt
    61021 gatccccacg aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt
    61081 ttttgctctt ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa
    61141 agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc
    61201 cgctcctgga agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc
    61261 aacaacgatc agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga
    61321 tgcagattcc ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga
    61381 aatagcgact tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc
    61441 tgagacagat actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa
    61501 gccagcattc tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata
    61561 agcatggatg atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc
    61621 ggcaatgggt agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat
    61681 ttgttccgta ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc
    61741 aaatcgggtt aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa
    61801 cctttttaag taatccaatt tctaaactcc tttacacaca attttacttc aagttttata
    61861 gtcgaaaatt tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa
    61921 actttaagaa aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta
    61981 atacagcaaa aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga
    62041 tagaacttga cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga
    62101 gatgtaaggc tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca
    62161 acctcttgat aaacggtgtt taaaagcttg catctgcggt aataagccca accaaattaa
    62221 aaacataaga agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt
    62281 acaacctctt ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg
    62341 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt
    62401 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
    62461 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa
    62521 accgatcatg atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc
    62581 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
    62641 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
    62701 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
    62761 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
    62821 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
    62881 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
    62941 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
    63001 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
    63061 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa
    63121 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
    63181 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
    63241 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
    63301 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
    63361 aatgatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
    63421 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
    63481 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct
    63541 gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat
    63601 cgtatcgatg acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat
    63661 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
    63721 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
    63781 attgcttagc aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt
    63841 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
    63901 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
    63961 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
    64021 tgccacttgt ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
    64081 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
    64141 taaatttttc acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt
    64201 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
    64261 tgctagttgc ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
    64321 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
    64381 ctcatctact ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat
    64441 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
    64501 attttcatct gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc
    64561 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
    64621 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
    64681 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
    64741 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
    64801 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
    64861 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
    64921 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
    64981 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
    65041 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
    65101 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
    65161 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
    65221 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagcc
    65281 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tatctattat
    65341 acgtatacgc aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa tgtttcagct
    65401 tttttcgttt tttattcctg tatttcttat ttttcgaatg acaggatgat tttcccagca
    65461 ggaggatgtc cttcaactag ctgatgtgct tggatgacat gcttaagtga agcagacaga
    65521 ttcattgaaa taaaaacatg aaaggctcct ttttggtagg ctttgattat gccagcaaag
    65581 gcttctgcat ctagatattc ctttcttgga acaaacgatt cataaaagcc ttcctttttt
    65641 tgccgcaaat ctagatctgg gaggtcattt aatgcaacat acctgccgcc tggtttcatg
    65701 atctggatgc cggtttctcc tttgatactt cctttcgtcg catcgattac aaggtctgcc
    65761 tgatcagcaa attgcagacc tggatttgtt ttatcataag cagcaaatgc tgaagctcct
    65821 aattttttta ccttttcctc atttttactg gatgcactgg ttaagattcg gattcccttt
    65881 tcatggagaa gctggatcag acttgacccg accgcacctg atgctcctag tatcattaca
    65941 gtatctgtcg gctggatttc cgtcaaatgg ttgattagat tataagccgt aattcctggc
    66001 gtgaccattc cggctgcttc ttcccaactc attttatctg gaatttttgc tacttttgct
    66061 gatggcaaca cgacttcttc tccgtaagtc ccgccgactg catggacagc tactcgatca
    66121 cctacatgaa cagttgtgac atctgaacca atctctgtca caataccagc gccatcatta
    66181 cctggtacat aagggaattt caatgttcgt atctctttca tttctcctaa acgcaaggca
    66241 acatcataag gattcacact gaatgctttt atttctacac gaacatgtcc agaaccgact
    66301 tctctgggat gagcatcgat ttcttcaaaa acatcttgtg cttctccgta accgttaatt
    66361 gcaaatcttc gcatggtcac catctccctt tttctttaag tttacgttta tgcctttatt
    66421 ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca aaacaaaata gttgtctttt
    66481 ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag attacgattt acgctaataa
    66541 aaaataattg gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac
    66601 agcaagaaac gattgaacgc tatgctgctt tacgccagga attaaaagcg caaaaagatt
    66661 acgaaggctt gcgtaaactt cctttagatg ctcgtccaga acgattgaaa aatcgtgatt
    66721 taatcgatgg acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc
    66781 gtgaattagc acataaaggt ttgattccag gagtaaaaaa agccagctgg taatccaagt
    66841 ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc ttggatcatc aaaaaagata
    66901 cgttcgcttc ttctagcaga aatgctggta ctagaagcag acgtatcttt ttttacgcac
    66961 agctggtcct tttttatatg taaaggctgc atggttactt cgctatattt atttagtaac
    67021 aggagtcagt gcttcgatac ccacactaat tttcaatgct cggtcaactt ctgccataat
    67081 gggtcgatcc aaatggcaga ccttttcttt caatcttgat ttatcgatcg ttctgatctg
    67141 ctccaacagg ataacggagt ctctttctat tcctgttcca gtagctttga tcccaatgtg
    67201 agtcggaagc ttaggtttag ccatttttgc cgttatggcc gcaacaatga tcgtcgggct
    67261 aaaatggtta cctagattat tttgaataac caaaactggg cgtgttccgc cttgctccga
    67321 tcccacgact ggagaaagat cagcaaaata aatatctcct ctttttacca taggatcctc
    67381 cttattcttg atattctctt ggtatccgct gtcctaatcc gcaagccact tcatagtgga
    67441 tcgtacctaa ttgatctgct atatcctgca tagtgatttc tttgtctgca tttttgccga
    67501 taagcgtcac ttttgttccg acaggaaatt cttgaggcaa tcggatcatc agttgatcca
    67561 tacatacacg cccaatcgtt tcacagtagt tcccttctac caatagagaa aaaccctgca
    67621 tctttcgtgg ccagccatct gcataaccga tcggtatcgt tccgatccac tctgcttcag
    67681 gagtgatata tgtttctcca tagccgattc cttcacctgc tgggagtttt ttgacttgaa
    67741 tcaattcaga aaccagctca agtgctggct gcaatggata aacttccggt agtgcgtgcc
    67801 cagagggatt aagtccgtac attgctactc cataacgaat catattgccg attgtttcat
    67861 catgccataa agccgttgca ctattgctga catgtacata tcgaggaagt tcgggaagtt
    67921 ttttcagcac ttctataaag cgttcttttt gcagattcca atatgtatca tctgcctgat
    67981 ctgctgtaga aaaatgggta aataaacctt cccattctaa tacacgactt tctttgatga
    68041 atgcagctgc ctcttttact tcttcaggtg tacaaaatcc gatacgaccc atcccagtgt
    68101 ctgcttttag atgaatcgaa agaggcgtct ctagcttact tttctctaaa acagcagctg
    68161 ctgcttgcaa ccattcccgt gtacctgcag ttactgataa atcatgagca acagctacag
    68221 tcagtgcgtc gtaaggaacc atattcaaga taaggatggg ctgagtaaat ccagcttctc
    68281 tcaactcgac tccttcatct aaatttgaaa cacaaaagcc cgaagctcca cctgcaactg
    68341 ctgcctcagc tgtttcaata gctccatgac cgtatccgtt cgcttttact actgcaaaca
    68401 attcttttcc ttgcggaagt cttgcaactt catttgctac attttcagtt atagcttttt
    68461 tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac catcgttttt ccttctttct
    68521 agtcttccaa aataatttgt gcaaaagctg tttcatttgt atgagtaata ctgacccata
    68581 cattgccttc atgaggtgaa cgcgtcacaa caggctgacc attttcattt tttaaaatct
    68641 cgacatcttg aaaggtaact tttccgatcc cagttcccca agctttagag aaagcctctt
    68701 tgcaagcgta acgcccgcct aaaaattcta cttggcgatg aaaaggcaag gactggaata
    68761 gtttcatttc atcagaggtc aagattcgtg caatgaattt tggtttttct tcgatcagcc
    68821 gagtgatcct aggaagttcg actgcatcga tcccaattcc ttttatcata tgcttttctc
    68881 ttttcaataa atttagaaca ctctcattct accaaattat aaccattttg ccacaaataa
    68941 gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga gaaactttca ttttatgttt
    69001 aatcgttatt tgaacggatt acaaatccgc gttttttatc attgtgacgt ttaccgccat
    69061 ctttttggct gttattattt ttgctataac cgccttttga tttgtttcca cgataattac
    69121 cgccgtcacg acgattacgg ttgtttccgc caccacgttt gccattttta ttgtatcctt
    69181 ttttctgcat tggcaatggg cgttctggtg tgatcttcac tggcactgca tcagctggat
    69241 cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg tgcagaatat tcttctaata
    69301 atttatctgc tgtttgcagg tatttatcta gaccattttc tgcaagtttt gtttcgattt
    69361 gttcaactgc agcacctaat tgacctttga atgcttcttt ttcggttggt ggacgtaaag
    69421 tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa gtagtccatt tcatttggtg
    69481 taacgaaagt cacagacata ccgccttttc cggcacggcc tgtacgtccg atacggtgaa
    69541 cgtagctttc tggatcttga gggatatcat agttgtacac atgagtgaca cctgaaatat
    69601 ctaatccacg tgcagcaacg tctgtcgcta caaggatatc taaatgaccg ctcttaaatg
    69661 aacgcaatac gctcattcgt ttttgttgag acaaatcgcc atggattcct tctgcacggt
    69721 aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg gcgttttgta cgtccgaaga
    69781 cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt catgatatcg aatttttcgt
    69841 attcttttgc gcggacatag tattgatcga tcaagtcagc tgtcatttct ttcgctttga
    69901 tcttcacatg atgtggattt ttcatgaatt taacgccaat attcttgata gctggcggca
    69961 tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac ttgtgaaatg attttttcga
    70021 tatcttccaa gaagcccata ttcaacattt catctgcttc atccaaaact aatgtttgaa
    70081 cggttccaag tttcaatgtg tggcggttaa tgtggtcaag catacgtcct ggtgtaccca
    70141 caacgatatg tggacgatct ttcaagccac ggatctgacg gccaatatct gctccgccgt
    70201 aaacggcttg tacacgtact tttttgtcgc gccctaaacg atataattct tcttgtgttt
    70261 ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg taattcgtga cgatctggat
    70321 cgattttttc taacattggt aatccaaatg cagctgtttt ccctgtacct gtctgtgctt
    70381 gtccaataac gtcttttcca gctaatgcta gtggaatcgt ttctgcttgg ataggcgttg
    70441 cttcttcaaa accagcgcgt tcgactgatt tcaacaattc tggtgataat tctagttctt
    70501 taaatttcaa atgaatcctc ctatatggtt gttaactgag gatgtagctt ttttgctacc
    70561 ccgtcgattt gttcggtcaa agtgtattgt ttctttttga cagtggccac gcacgttttt
    70621 atccattctt agcccctgtg gcaccaccga aaaatcactt tgcgaagttt aggcattaga
    70681 aaagccaaac gggtcgcgtt cagctttatc attattatga catctggctt caacaagttg
    70741 tttctatggc aaaaccagtc caatttctct tcatctttcg gcaataggtt attctaacat
    70801 agtttttcgt tttctgcaag tttacagatt tttatttcat ttgctgaagt tgttctacca
    70861 cttcaaataa gcccatgctg ttgctgcctt ttagcataat actatctgtt gcttcaagat
    70921 tttgtttgat agcatcaatc aatttattct tttctttttc aaaatagaac acaggtagtt
    70981 gggggtattt ttggttcaat tcttctttta gtgcatgcat ttgttcccca taaaggaaaa
    71041 tcatggcgaa tttctcagga tcgatatgtt cactcatttg cgcatgcata tttaaagact
    71101 ctttccctaa ttccagcata tctgctaaca cagcgattct gcgtcctttt gttggcagat
    71161 ttttaaaagt atctaatacc agtcccatgg ctgttggatt tgcattgtag acatcgctca
    71221 aaatagctgc accatttcct gccgttagcc attcggttct gttttgagtg atagaaacat
    71281 gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa gaagcgcccg atcgtatacg
    71341 caatcaaagc attcgttaca ttataacttc caggcagcgg gatcgtaaat gtttctcctt
    71401 ctactacaaa atcggtttgt gcttttcctt ttgctgtcac ttccgcatga acatcgccat
    71461 ttccaatacc gaatgttgtg actgtttgta ctaatgttgc cgttaaaggt gtcagcaacg
    71521 gttcatctgc gggcacaacg agtaatccgt ctgatacaag tccgtcagtg atttccattt
    71581 tggctttagc gattccttct cgcgacccaa gattttctat gtgtgcttca ccgatcattg
    71641 tgattgctgc agcttttggt tgagccataa gtgaaagttc cgtgatttca ttggcgtgat
    71701 ccatgcccat ttccaaaacg atcttttctg tatcttgggg catgtgcaaa atcgtgtaag
    71761 gaagaccaat attattgttg taatttcctt gcgtcttata tgttttgtat ttttcagaca
    71821 agacagacgc ggttagatct ttcgtggtgg ttttaccatt gcttcctgta attgcaacga
    71881 cttctgcgcc tactttttcc aagtaatagg ttgccaattg ctgcatggct ttcaaaggat
    71941 ctgctacagg aataactgga aaattttttg gagcttgttc aacaggcaag ctccaaaatg
    72001 aagcagatgc tccgtttgtg atcgcttgct ggataaattc atgtccatct cgcgtaccag
    72061 caagaggaac gaataaactt ccttcggtta tcttccggct gtcaaattcg acattggtaa
    72121 tctttgtcag tgcagagcat tcgctgccca cggcttgtgc aatttcttgt attgttagat
    72181 tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt ttggcctaaa cggcgggttc
    72241 gcatgactct cacttccaag taaatttctc tattcgtatt tagaagctag aatgaacgcg
    72301 gttacgcaat cactccagcc tcttctttat ttctctttat tcattttttt caaaaaaaga
    72361 ttgacgctga tggtatcgat tcattcctaa ctggatcagt tcttcaatca aatcaccgta
    72421 tttcaagccc atattttccc ataagagtgg gtacatactg aactccgtaa atcctggcat
    72481 agagtttaat tcattcagga ataattcatt tttatttgtc aaaaagaaat cgcaccggct
    72541 caatccgctt ccacctaaca tcgtgtaagc taacttcgcg tactcttgcg ctttttgata
    72601 aacttcttct ggcacttcgg ctggaatctg catttcgatt ttattattga tatattttgc
    72661 ttcataatca tagaatgcta cgtcttttac gacttcacca ggcaaagtcg tccgaacgtc
    72721 ttcatttcct aatacagcaa cttcgatttc gcgcgcttca attccttgtt caacgattgc
    72781 tcgagaatca tactgatagg ctgttgctaa agcattttgc agctcttctc ggttttctgc
    72841 ttttgtaatg ccgacactag aacccatatt cgccggtttg acaaacatcg gataaagcaa
    72901 agaaccttca cattgatcaa atactttttt aggattttct ttccattgat tcttaagtac
    72961 tggtacataa ggaacttgcg gcacaccagc agcttgtaaa atatacttgg tcatgatttt
    73021 atccatggca catgcactgg tcaatacgcc tgcgccgaca taaggcatat tcaatgtctc
    73081 taagaagcct tggatcgttc catcttcccc gtttggccca tgtaaaactg gaaaaacgat
    73141 ggctccttct tctttgattt cgcccggatt gatcactttt cctgtaaagc cttcctctgt
    73201 ctgtccactt gggtcccatg aaagatgcaa gacatctttg ctggcaggtt tttctgttag
    73261 taatggacct ttgacccatt gtccttcttt tgtaataaat acgagttgaa cttggtaata
    73321 attataataa atggcattta aaactgaaaa tgcggaaaga atggacactt catgctctgc
    73381 gctgcgtccg ccatatagta aagtaatctt caaaagattg tcctcctgta ttctaaatcg
    73441 ttcagtgatt tactctcata ttttagcaca aaacaaaaat aaacgaataa cttatttata
    73501 aaaaaacgag ccagcttcta aaagaacgag cgtcatttat tttttgtcag ctagattatt
    73561 tgtcccgttt tttataaaaa gttatgataa agaaaaggag ggatgatgat ggcgcaaaca
    73621 aatccacgca gccgcttggc agaaatcatt cgagtgttca tcaattacaa tgtagtgcca
    73681 aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag cctttgaaga actaggcccc
    73741 accttcatca agataggaca gatgctttct gttcgagaag atttgctttc ttctgccttc
    73801 actcaaacat tcaaaacatt acaagatagt gtacctagcg acacattttc aacggtcaaa
    73861 aagacgatcg aaacagaact ttctctttca ttatcagata tattcgatga tttttcaaaa
    73921 tcgccttttg cctctgcttc tatggggcag gctcatcgtg ccaagctgaa gaacggtgac
    73981 gcggtagtcg ttaagatcca acaccctaac atcgccgaag agatccgact tgatcttcaa
    74041 ctatttgaac gagcgatccc tttgatcaaa tacattcctg aaactagcgt agttgatcta
    74101 aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg aaatggactt cttaaaagaa
    74161 agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct ggaaagagat ccgcagcccc
    74221 aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga tggaagaaat gtctggtaaa
    74281 aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa cttttatcac tggaattcaa
    74341 aacaagcagc tgaaacaaga agttgcaaag cttttagttg agaactttat gaaacaggtg
    74401 tttgatgatg gttttttcca tgctgatcct catccaggga atctgctatt ccatgtacta
    74461 acaaaagaag aacagacaca agctagccga aaaactgaaa cagtccatga aaaagagttc
    74521 ggttcctttg cttttcgagc ctccacttct gcagaagacc ctgttgcgcc atataccatc
    74581 aactacatcg attttggtat gatggggcat ctttctgcag gacttaggca aaaactcaca
    74641 caagccgttt tagcgctgta tacgaaagat gcttatcgaa tcgaaaaagc ggtccttcga
    74701 ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc accaagaact gacaagtttt
    74761 cttgaacagt attatgattc tcctattgat gaaatcaact tgcaagaagt attcacacat
    74821 gtcgtgacga tttgtcatca aaataatctg cagttcgatc gtgatatcac gcttttgctg
    74881 aaagcatttg ggacattaga aggggtgatc cgcgtcctcg atcctgaagt ttccctcatg
    74941 gaagtcgctt cgccttttgc tcaacattat tttctcactc atctggatgt agaagataca
    75001 ttgaaacaaa gcggattaga cttattggaa gggatgaagg ctgcgcctaa gatccctcaa
    75061 cagctccacc atctattaga aatgtggact tcgggacaag gaaaagtcaa tttggaattg
    75121 aaaaagcaag acaaattatt gagcaggatc gaaagtatga tcaatcggct tgttttcggt
    75181 atgattttag ctgcattgat cgtagggtcc tccctgcttg tccaagcagc acctgtagaa
    75241 aacgcagagg tcgtttcctt attagggata tttacgtatg ctattgccgc attcgtcatt
    75301 atttttttgg caatcgatgc attgatccaa atgtataaga agaggaaaaa gtagaaaaag
    75361 gttgtgacag aaagaatcag cttcaagaaa taagaagggg ttttacataa attgtttctc
    75421 aaatttttgt gaaaattcgg cttatttttc gaagaggcta cttctgaaac accgagaata
    75481 aaaaatgtga aaaagctgtt ctgcacccag taataagaac aatcaaaaag ggaacatgga
    75541 acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa taaaatacct
    75601 tcgttaccta ctatcttctg tacttggtaa accttagtca attgcctttt ttaacgcttg
    75661 acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc ttatgccagc
    75721 ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat tcgcttcgtc
    75781 aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa gtgaagctaa
    75841 accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga ttttataatc
    75901 cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt attctttggt
    75961 cgtataatgg tccacactgg tcacgataac cacgtcaaga ccataggatt ctcctaattt
    76021 tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca ttctttttcg
    76081 ccttcttcca taaatgataa acagttacta atccgctcag taaaagacca acattgatcc
    76141 attttgccaa gataagcagt tgtacacttt ctatcggcac tgattcatgg ttcagcgtga
    76201 tcaataatag atggattggc tctcccattt cttttgctaa aacaaacaaa agcgtcatca
    76261 ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca acgatccggc
    76321 gatccattgg aatagctgta tcagtttgat aatattcctt atttagccag ctgcctgtta
    76381 ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga atcaagagga
    76441 ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat gttaaacgtt
    76501 tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa ggcaaataca
    76561 taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg ctaaataaaa
    76621 aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag cttggcaact
    76681 tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc agcaagaaac
    76741 cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt ctcaaaagaa
    76801 acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc agatttcttt
    76861 tgatgtcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg taggccatga
    76921 cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca ggcaactcaa
    76981 tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc ccatccatat
    77041 tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt acaagccatg
    77101 ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa aatacgccta
    77161 atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc aacgtgtaac
    77221 tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga aaggcataaa
    77281 acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc aaaaataaaa
    77341 gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt gtaaaattac
    77401 gcccaaatcg tttgagtttt tcgaacatga caatggctcc ttaaacagtc ttataactaa
    77461 tctgctcttc gattttagca ttaaagggga aataaaccaa ttttacagaa tatattcttg
    77521 taaaaaaaaa tacagaaaga agcataagct tctttctgca tttttacggt acttgattac
    77581 tcaagcagac aagcccttct ccacaaaagt cttgcccgac acccctgcat ccggcaggtg
    77641 ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag
    77701 agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt tatgcgagag
    77761 cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa ataagttctt
    77821 ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc ccctgattca
    77881 ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa
    77941 ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta cttaaataaa
    78001 tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg ctttttaata
    78061 aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac tgataagttt
    78121 tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaaacttcc ggccatactt
    78181 cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt ttttccatac
    78241 acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa aagagcatga
    78301 tcatgctata cacacccacc gaccacggtt tcaagaaaat cacaatctgc ataaattccc
    78361 taaaagataa tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc gcaaagggaa
    78421 gcagcaaggt aacaactagt acttttttaa tatctttcgt ttttcttgtc agcaattcat
    78481 caaatttctg atactttttt ggcgataaaa taagctggac aaaggctacg ccatagtaac
    78541 ggacaagcaa aaatccgatt gcttccccaa tcactaggcc aatacaacta tataaaagcc
    78601 caaatccatt gccaaacatc agcatgcccg ctactgagga gatccctccc ggcaaaatag
    78661 gaatgatcac ttggatcact tgcaacagaa caaatatcat cactgcatac ttgccaaatt
    78721 gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga taagcataaa
    78781 gaatcaacac aaagaaaaaa aacagaccaa taatgggcat gatctgaaga catttttgta
    78841 aaactgatcc ttttttagac atctatattc cccacatctt ttttcattta ttaaaactgc
    78901 ctc
//
LOCUS       DADQRX010000003        75412 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.003, whole genome shotgun sequence.
ACCESSION   DADQRX010000003 DADQRX010000000
VERSION     DADQRX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 75412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.003"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1005)
                     /locus_tag="J8V14_000175"
     CDS             complement(<1..1005)
                     /locus_tag="J8V14_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920314.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     S"
     gene            complement(1260..1769)
                     /gene="mreD"
                     /locus_tag="J8V14_000176"
     CDS             complement(1260..1769)
                     /gene="mreD"
                     /locus_tag="J8V14_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBA0920315.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1773..2627)
                     /gene="mreC"
                     /locus_tag="J8V14_000177"
     CDS             complement(1773..2627)
                     /gene="mreC"
                     /locus_tag="J8V14_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBA0920316.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2782..3420)
                     /locus_tag="J8V14_000178"
     CDS             complement(2782..3420)
                     /locus_tag="J8V14_000178"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBA0920317.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3421..4071)
                     /locus_tag="J8V14_000179"
     CDS             complement(3421..4071)
                     /locus_tag="J8V14_000179"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA0920318.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4084..4983)
                     /gene="rsgA"
                     /locus_tag="J8V14_000180"
     CDS             complement(4084..4983)
                     /gene="rsgA"
                     /locus_tag="J8V14_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBA0920319.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5148..7217)
                     /gene="pknB"
                     /locus_tag="J8V14_000181"
     CDS             complement(5148..7217)
                     /gene="pknB"
                     /locus_tag="J8V14_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBA0920320.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7214..7954)
                     /locus_tag="J8V14_000182"
     CDS             complement(7214..7954)
                     /locus_tag="J8V14_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBA0920321.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7967..9325)
                     /gene="rsmB"
                     /locus_tag="J8V14_000183"
     CDS             complement(7967..9325)
                     /gene="rsmB"
                     /locus_tag="J8V14_000183"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBA0920322.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9325..10263)
                     /locus_tag="J8V14_000184"
     CDS             complement(9325..10263)
                     /locus_tag="J8V14_000184"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBA0920323.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10256..10747)
                     /gene="def"
                     /locus_tag="J8V14_000185"
     CDS             complement(10256..10747)
                     /gene="def"
                     /locus_tag="J8V14_000185"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBA0920324.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10762..13170)
                     /gene="priA"
                     /locus_tag="J8V14_000186"
     CDS             complement(10762..13170)
                     /gene="priA"
                     /locus_tag="J8V14_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBA0920325.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13423..13728)
                     /locus_tag="J8V14_000187"
     CDS             complement(13423..13728)
                     /locus_tag="J8V14_000187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBA0920326.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13733..14347)
                     /gene="gmk"
                     /locus_tag="J8V14_000188"
     CDS             complement(13733..14347)
                     /gene="gmk"
                     /locus_tag="J8V14_000188"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBA0920327.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14834..15118)
                     /locus_tag="J8V14_000189"
     CDS             complement(14834..15118)
                     /locus_tag="J8V14_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920328.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15136..16026)
                     /locus_tag="J8V14_000190"
     CDS             complement(15136..16026)
                     /locus_tag="J8V14_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBA0920329.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16046..16264)
                     /locus_tag="J8V14_000191"
     CDS             complement(16046..16264)
                     /locus_tag="J8V14_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920330.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16234..17934)
                     /locus_tag="J8V14_000192"
     CDS             complement(16234..17934)
                     /locus_tag="J8V14_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBA0920331.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18201..18974
                     /locus_tag="J8V14_000193"
     CDS             18201..18974
                     /locus_tag="J8V14_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920332.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19055..19624)
                     /gene="thiT"
                     /locus_tag="J8V14_000194"
     CDS             complement(19055..19624)
                     /gene="thiT"
                     /locus_tag="J8V14_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBA0920333.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19690..19792)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19798..20769)
                     /locus_tag="J8V14_000195"
     CDS             complement(19798..20769)
                     /locus_tag="J8V14_000195"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBA0920334.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20863..22236)
                     /gene="glmU"
                     /locus_tag="J8V14_000196"
     CDS             complement(20863..22236)
                     /gene="glmU"
                     /locus_tag="J8V14_000196"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBA0920335.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22739..23557)
                     /gene="purR"
                     /locus_tag="J8V14_000197"
     CDS             complement(22739..23557)
                     /gene="purR"
                     /locus_tag="J8V14_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBA0920336.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23724..24548)
                     /locus_tag="J8V14_000198"
     CDS             complement(23724..24548)
                     /locus_tag="J8V14_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBA0920337.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24521..25201)
                     /locus_tag="J8V14_000199"
     CDS             complement(24521..25201)
                     /locus_tag="J8V14_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBA0920338.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25224..26162)
                     /locus_tag="J8V14_000200"
     CDS             complement(25224..26162)
                     /locus_tag="J8V14_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBA0920339.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26407..27258)
                     /locus_tag="J8V14_000201"
     CDS             complement(26407..27258)
                     /locus_tag="J8V14_000201"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBA0920340.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27412..29985)
                     /gene="mprF"
                     /locus_tag="J8V14_000202"
     CDS             complement(27412..29985)
                     /gene="mprF"
                     /locus_tag="J8V14_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBA0920341.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30126..30932
                     /gene="proC"
                     /locus_tag="J8V14_000203"
     CDS             30126..30932
                     /gene="proC"
                     /locus_tag="J8V14_000203"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBA0920342.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31011..32567)
                     /gene="rny"
                     /locus_tag="J8V14_000204"
     CDS             complement(31011..32567)
                     /gene="rny"
                     /locus_tag="J8V14_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBA0920343.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32978..34027)
                     /gene="recA"
                     /locus_tag="J8V14_000205"
     CDS             complement(32978..34027)
                     /gene="recA"
                     /locus_tag="J8V14_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBA0920344.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34136..35377)
                     /locus_tag="J8V14_000206"
     CDS             complement(34136..35377)
                     /locus_tag="J8V14_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBA0920345.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35617..36195)
                     /gene="pgsA"
                     /locus_tag="J8V14_000207"
     CDS             complement(35617..36195)
                     /gene="pgsA"
                     /locus_tag="J8V14_000207"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBA0920346.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36404..37288)
                     /locus_tag="J8V14_000208"
     CDS             complement(36404..37288)
                     /locus_tag="J8V14_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0920347.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(38066..39352)
                     /locus_tag="J8V14_000209"
     CDS             complement(38066..39352)
                     /locus_tag="J8V14_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBA0920348.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39345..40613)
                     /locus_tag="J8V14_000210"
     CDS             complement(39345..40613)
                     /locus_tag="J8V14_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBA0920349.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40865..41326
                     /gene="mscL"
                     /locus_tag="J8V14_000211"
     CDS             40865..41326
                     /gene="mscL"
                     /locus_tag="J8V14_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBA0920350.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41385..41831)
                     /locus_tag="J8V14_000212"
     CDS             complement(41385..41831)
                     /locus_tag="J8V14_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920351.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41924..42169)
                     /locus_tag="J8V14_000213"
     CDS             complement(41924..42169)
                     /locus_tag="J8V14_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920352.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42284..42862)
                     /locus_tag="J8V14_000214"
     CDS             complement(42284..42862)
                     /locus_tag="J8V14_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBA0920353.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42939..43469)
                     /locus_tag="J8V14_000215"
     CDS             complement(42939..43469)
                     /locus_tag="J8V14_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBA0920354.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43473..44333)
                     /gene="rlmB"
                     /locus_tag="J8V14_000216"
     CDS             complement(43473..44333)
                     /gene="rlmB"
                     /locus_tag="J8V14_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBA0920355.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44326..44721)
                     /locus_tag="J8V14_000217"
     CDS             complement(44326..44721)
                     /locus_tag="J8V14_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBA0920356.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44718..46130)
                     /locus_tag="J8V14_000218"
     CDS             complement(44718..46130)
                     /locus_tag="J8V14_000218"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBA0920357.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46127..46663)
                     /gene="cysE"
                     /locus_tag="J8V14_000219"
     CDS             complement(46127..46663)
                     /gene="cysE"
                     /locus_tag="J8V14_000219"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBA0920358.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(46985..48445)
                     /locus_tag="J8V14_000220"
     CDS             complement(46985..48445)
                     /locus_tag="J8V14_000220"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBA0920359.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48615..49772)
                     /locus_tag="J8V14_000221"
     CDS             complement(48615..49772)
                     /locus_tag="J8V14_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBA0920360.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49806..51176)
                     /gene="radA"
                     /locus_tag="J8V14_000222"
     CDS             complement(49806..51176)
                     /gene="radA"
                     /locus_tag="J8V14_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBA0920361.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51239..51766)
                     /locus_tag="J8V14_000223"
     CDS             complement(51239..51766)
                     /locus_tag="J8V14_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBA0920362.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51996..52802
                     /gene="proB"
                     /locus_tag="J8V14_000224"
     CDS             51996..52802
                     /gene="proB"
                     /locus_tag="J8V14_000224"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBA0920363.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52799..54046
                     /locus_tag="J8V14_000225"
     CDS             52799..54046
                     /locus_tag="J8V14_000225"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBA0920364.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54122..54466
                     /locus_tag="J8V14_000226"
     CDS             54122..54466
                     /locus_tag="J8V14_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920365.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54583..54909)
                     /locus_tag="J8V14_000227"
     CDS             complement(54583..54909)
                     /locus_tag="J8V14_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920366.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55524..56726)
                     /locus_tag="J8V14_000228"
     CDS             complement(55524..56726)
                     /locus_tag="J8V14_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0920367.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56843..57358
                     /locus_tag="J8V14_000229"
     CDS             56843..57358
                     /locus_tag="J8V14_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA0920368.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57557..58003)
                     /locus_tag="J8V14_000230"
     CDS             complement(57557..58003)
                     /locus_tag="J8V14_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBA0920369.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58050..59528)
                     /gene="msr"
                     /locus_tag="J8V14_000231"
     CDS             complement(58050..59528)
                     /gene="msr"
                     /locus_tag="J8V14_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HBA0920370.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59882..60511)
                     /locus_tag="J8V14_000232"
     CDS             complement(59882..60511)
                     /locus_tag="J8V14_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBA0920371.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60495..60959)
                     /locus_tag="J8V14_000233"
     CDS             complement(60495..60959)
                     /locus_tag="J8V14_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0920372.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61446..62714)
                     /locus_tag="J8V14_000234"
     CDS             complement(61446..62714)
                     /locus_tag="J8V14_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HBA0920373.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62945..63682)
                     /locus_tag="J8V14_000235"
     CDS             complement(62945..63682)
                     /locus_tag="J8V14_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0920374.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63749..64318)
                     /locus_tag="J8V14_000236"
     CDS             complement(63749..64318)
                     /locus_tag="J8V14_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBA0920375.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64446..65318
                     /locus_tag="J8V14_000237"
     CDS             64446..65318
                     /locus_tag="J8V14_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0920376.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65660..66532
                     /locus_tag="J8V14_000238"
     CDS             65660..66532
                     /locus_tag="J8V14_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0920377.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66529..68487
                     /locus_tag="J8V14_000239"
     CDS             66529..68487
                     /locus_tag="J8V14_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0920378.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68819..69670)
                     /locus_tag="J8V14_000240"
     CDS             complement(68819..69670)
                     /locus_tag="J8V14_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0920379.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69793..70260
                     /locus_tag="J8V14_000241"
     CDS             69793..70260
                     /locus_tag="J8V14_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBA0920380.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70523..71005)
                     /locus_tag="J8V14_000242"
     CDS             complement(70523..71005)
                     /locus_tag="J8V14_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA0920381.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71105..71968)
                     /locus_tag="J8V14_000243"
     CDS             complement(71105..71968)
                     /locus_tag="J8V14_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA0920382.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            72566..73441
                     /locus_tag="J8V14_000244"
     CDS             72566..73441
                     /locus_tag="J8V14_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920383.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(73586..73738)
                     /locus_tag="J8V14_000245"
                     /pseudo
     CDS             complement(73586..73738)
                     /locus_tag="J8V14_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(74006..75337)
                     /locus_tag="J8V14_000246"
     CDS             complement(74006..75337)
                     /locus_tag="J8V14_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0920384.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        21348 a        16057 c        13423 g        24584 t
ORIGIN      
        1 tgattcttct gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc
       61 aggcgctgtt gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga
      121 ttgtgtagct gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt
      181 tgctgagctt tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc
      241 gcgtgcttct ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg
      301 ttgttgttca cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc
      361 agctttcttg tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa
      421 atcagcttgt ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc
      481 taattctttt agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg
      541 tttttgttgt tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg
      601 tccaatagca tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt
      661 gttgcttact tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg
      721 ttcttgtaaa tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg
      781 ttttgctaat aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc
      841 gatttgtgat tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg
      901 ttgtatctga gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa
      961 ggtcattgaa cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga
     1021 ctggcttaat ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg
     1081 taccatagca gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt
     1141 ttaaaaatga caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta
     1201 gcatttctta agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat
     1261 tattcatcac tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc
     1321 aacagcagtg taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat
     1381 ttaaaaatca actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata
     1441 ctgaaaaatt cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat
     1501 acaattaaag ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag
     1561 atagccccga gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa
     1621 cataataatg ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc
     1681 cctagcattc ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga
     1741 tagtacttca ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc
     1801 gtcacaactg acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc
     1861 ccataactat ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc
     1921 cctccaagac cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat
     1981 tgggtcacca ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg
     2041 ccattagccg aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt
     2101 aattcgattt tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc
     2161 gacatgactg ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg
     2221 tcctgccaac tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc
     2281 agcgtctgat tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg
     2341 ttttgctgtg aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca
     2401 taagttgaaa aaagattatg gacagaatct actccatttc gtacccaccg tactggggca
     2461 gagattgttt tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc
     2521 tttccttcat tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca
     2581 ataaccaagg taataataat atttttatta ggattgaact tcttcacaaa gacacctcga
     2641 attaattaga ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg
     2701 ctcctcttaa gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa
     2761 atttttttat ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga
     2821 aaaagaaaat tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc
     2881 tacttcttta ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt
     2941 caagcagcaa taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg
     3001 caaataataa gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt
     3061 gaagcgaggt tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt
     3121 agctccaatc aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa
     3181 ctgtgtatct gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa
     3241 tacttccttc tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg
     3301 gtagcctgct tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt
     3361 ggacagcaga gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat
     3421 ttagtctaat gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat
     3481 atatgatcct gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg
     3541 aacgattccg ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc
     3601 gagttccgta attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt
     3661 gacagtcatg actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg
     3721 tgttcctgga ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc
     3781 ccgatgaata tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc
     3841 aaatgctgga atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt
     3901 agtaacagga cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg
     3961 gccatccatt acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc
     4021 tctttgtaaa ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc
     4081 ctcctatttt cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg
     4141 taagaattgc aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg
     4201 ttttacttcg cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga
     4261 agcttctaca aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc
     4321 aatcgagcta aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac
     4381 atgacgtgtt gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt
     4441 cagttccggt gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa
     4501 gaccgatagc cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg
     4561 tacaatcacc ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg
     4621 gtcgtctgac aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa
     4681 ggtaactaga aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac
     4741 aatgattcca agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa
     4801 gatttccaga agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac
     4861 aagcggcgtg attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc
     4921 gtttgcgtat acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc
     4981 cacctccgtt tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa
     5041 cttctcaaag tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat
     5101 gcaacaaatc aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt
     5161 tctgaggtgc tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt
     5221 gtgctactgg agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa
     5281 gacgctggat ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc
     5341 gttcctgccc ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg
     5401 gaagtctcat gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt
     5461 tttgtatagc cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg
     5521 atgtaagttt cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct
     5581 gatggtacag tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt
     5641 gtaacaagcc aattacgaac agtttcgtag ttttctccta catgactact caaagtcact
     5701 gtttctggtc ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta
     5761 tcttctgatg gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct
     5821 tctttttgaa tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca
     5881 taacttttac cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga
     5941 gtgacttcgc gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta
     6001 ccttcttcga ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct
     6061 tttgcgagtg tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact
     6121 tctcctcgcc cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata
     6181 gctaatagga tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct
     6241 tcctcctctg gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat
     6301 gaatcaggca ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc
     6361 acatgaggct gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca
     6421 tcagacattt cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa
     6481 atcacgtttt ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca
     6541 tcttgaaaat gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc
     6601 gtcaacatct cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata
     6661 cttcctctgg cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc
     6721 gtgatcgatg tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct
     6781 tcattatcga tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga
     6841 gctaaagaaa tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca
     6901 ggataatgtg tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga
     6961 tactgcatgt tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt
     7021 tctgttgctg ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag
     7081 tcaaatcgta agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat
     7141 acatttgcca taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt
     7201 ttgcccaact cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat
     7261 ccgctccgcc tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc
     7321 cttcttgatt gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg
     7381 aacataacag cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg
     7441 ttccaggcat gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt
     7501 cttcgctgat ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt
     7561 gcatgatttt atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga
     7621 accagtcatc tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct
     7681 gttcttgtcc acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt
     7741 gggcgatttt ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta
     7801 ccgccatctg actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag
     7861 caagcttgag acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc
     7921 gtcttcctac atctgattga tattcaatct gcattttttc acctcattac tttttgcgca
     7981 aacaacaaat gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct
     8041 cttcgattga tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt
     8101 gtctttccaa gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc
     8161 tataagtcag aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt
     8221 ggatttttgg taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc
     8281 gccgaagcag cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg
     8341 cttcgaactg tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga
     8401 cacctagacg tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag
     8461 cgaccacttc tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc
     8521 cgggtgctgc acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta
     8581 caagcatcga actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag
     8641 ctaaatgccc ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga
     8701 tctcttcttc ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc
     8761 gggcactagc atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc
     8821 cgatttgatc caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc
     8881 tttcgatagg atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca
     8941 agacaccatt tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg
     9001 cctcgtttaa aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa
     9061 gataaagaag catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa
     9121 cgtaaaattc caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac
     9181 ccacatcttt ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt
     9241 aggcgccacc cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt
     9301 tcattttttt agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc
     9361 gcttccgtta aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt
     9421 gatggctaag acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat
     9481 gatcgtacca ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca
     9541 gcgtatgccc tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt
     9601 gtcaatttct tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg
     9661 cgaaaaagtt gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg
     9721 caatgtttct agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac
     9781 atcatcttgt ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt
     9841 tttgatcatt tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg
     9901 aacaggcgca cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa
     9961 ttttggtact tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac
    10021 atcaggcacc aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg
    10081 cagcacacgg ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg
    10141 tttccgccca actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc
    10201 tatcagccct tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt
    10261 catcaagatg ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt
    10321 tctcaatgaa caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat
    10381 agccaaaagc cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt
    10441 catctgctcg cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa
    10501 ggctttcacc ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat
    10561 ctacttcgat cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc
    10621 cgtcattcgc aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga
    10681 ttacatcgat tggctgagca gttcttttta atttatcatt agggtggatc aatataggat
    10741 agcgcataat ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa
    10801 aagaccttgt cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag
    10861 attcggttca tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa
    10921 aatcatgctg ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg
    10981 attggcaatc tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat
    11041 tttcacagta aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg
    11101 ataaaactgc tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa
    11161 tgtttggatc acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt
    11221 aagcaattgg aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc
    11281 attcaacacc ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt
    11341 ccctaataat atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc
    11401 ttttcttcga gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg
    11461 gagttcctct tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca
    11521 attaggacag cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg
    11581 tgtatccata tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca
    11641 gtccctacac atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc
    11701 ttttttgtct aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg
    11761 atgattctga aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc
    11821 tttggctcgg tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga
    11881 ttctaaagag ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca
    11941 aattgctaga tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg
    12001 ttcttcatcg ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc
    12061 gcctactact acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc
    12121 ttgggaaagt ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc
    12181 gaccatttgc ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga
    12241 caaaacatca gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc
    12301 cagcaaaaag acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt
    12361 tttttgttct gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg
    12421 gtcgcgataa cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa
    12481 gacagcagta ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc
    12541 agataaacac aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc
    12601 ttcttcgatt tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt
    12661 tcgtgtatgg acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg
    12721 agaaaggctg ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa
    12781 tacctgaact tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat
    12841 cacactcgga agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat
    12901 ataatcagct aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat
    12961 aactgccttc aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc
    13021 ttggacatga cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc
    13081 tgtttccatt tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac
    13141 gtccacaatg acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct
    13201 tatcaagcga caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct
    13261 tctgccaaat cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa
    13321 gttcagacac agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct
    13381 gggtttgctt ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc
    13441 acggatacgt gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc
    13501 ttccatcttc aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc
    13561 atcgatttct tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg
    13621 ctgtgcactt gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt
    13681 tgaatttaca cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct
    13741 cctttaacat tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa
    13801 taatgttttt gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt
    13861 aaagagccat catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat
    13921 ctgcatctgt tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca
    13981 aaaagatgaa tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa
    14041 tctccaagaa aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc
    14101 cataataatt cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt
    14161 caaactcttc tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt
    14221 tacgggtagt cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt
    14281 ttcgtactgt gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct
    14341 ctgacatgac gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt
    14401 tttctgcaac tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct
    14461 gtaccaagag gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa
    14521 cagcttctca tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc
    14581 cgtgtataag aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa
    14641 aatagtttct cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca
    14701 tcacttattc aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat
    14761 tcctagagca aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg
    14821 ttcagcatcc ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat
    14881 cttttcgaga acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa
    14941 taaatttccg attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct
    15001 cattatagtc tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat
    15061 cccgtttaac cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta
    15121 tctctacctc tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt
    15181 cttagactgg acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt
    15241 gatttcccga ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc
    15301 ttcgataagt tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat
    15361 atctccgcgt tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc
    15421 tccaagaaat tcctccagct ttttttgata ccttatttga aattcctttt catagatttc
    15481 tacaaaacta tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac
    15541 taaagctaaa gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt
    15601 caatgtatct agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac
    15661 cgttacgaag ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt
    15721 ttgactaccg tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa
    15781 ttcccagttg atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac
    15841 ttcgattcga ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa
    15901 gttcaggatt ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat
    15961 ttcaatatct aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact
    16021 tttcattcga cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa
    16081 actcttcgtt cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc
    16141 gaagatcgaa ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc
    16201 ccttgttgtt ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg
    16261 ccatggttgt tcctccttga gataatataa atataggatc agtaatgtgt caactggttg
    16321 cttaagcaat tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata
    16381 aacagttttc gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc
    16441 ttcagtactt aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg
    16501 ttggcagatc atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg
    16561 tttttttgct aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt
    16621 atataaatta cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg
    16681 aatttctttt gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga
    16741 atgaaatgta taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc
    16801 taattgtaaa tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca
    16861 caatgtttga catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc
    16921 tggcccttgc acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat
    16981 aggtattttg ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc
    17041 catagcggga taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt
    17101 ctgttggata tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa
    17161 acgtattttg ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc
    17221 caatggagca gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc
    17281 tcgatagaag cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg
    17341 tcttgtcgac agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa
    17401 tgcgccgttc aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc
    17461 cttcagcgcc tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac
    17521 aagtgctgta tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt
    17581 atgaaaaata cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc
    17641 tttaataaaa aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt
    17701 ttcaccttca atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa
    17761 atagacggta tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca
    17821 aaaagcgtct ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg
    17881 gataggcgaa tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct
    17941 ctcctttttc ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact
    18001 ttgtagattc cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat
    18061 cacaaaaatt aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt
    18121 ttaaaataaa agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag
    18181 gaaataagag gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta
    18241 atgctgtttt aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc
    18301 ctcaaaactt gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag
    18361 ggctgaaagt aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca
    18421 ggcgtggatt cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat
    18481 tgacaccttt agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt
    18541 cgccgttttt ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga
    18601 atcggattta ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt
    18661 cgatcttgtc agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg
    18721 aaaaaatcag tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt
    18781 tttcttttga tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg
    18841 aagattatat ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt
    18901 taatctactc tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc
    18961 tgccattgaa gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca
    19021 tgattctgtc tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca
    19081 caaaaatcga aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg
    19141 tactcaaccc gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata
    19201 gtgcatacgc tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg
    19261 cgccaatcaa tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat
    19321 agcctttttg gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg
    19381 ggtactcaat caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta
    19441 ggatgcccca aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa
    19501 gagtcaatgg aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca
    19561 acgacaacac catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc
    19621 ccataaaaaa acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa
    19681 atagtcaaac agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag
    19741 ggtttagaaa tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta
    19801 tttatttttt gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc
    19861 atctcccatt agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg
    19921 aagatagatc gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa
    19981 agcagggccg gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc
    20041 tttcaaagca ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag
    20101 tacacaagtt ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt
    20161 tggacggcgt ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc
    20221 ccgtgtgaca ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt
    20281 ttctaagaaa taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa
    20341 gaaaccttgg atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt
    20401 ttgaatcatg tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc
    20461 ttgacgcgca tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt
    20521 cagtgcgtca atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga
    20581 ttgaataaca tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc
    20641 atcactaaat tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt
    20701 ctctgctaat gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg
    20761 ttttgacatg gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag
    20821 tagtcatatt ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag
    20881 tttttttgca tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc
    20941 tggaatattg tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac
    21001 aggtccgatg atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg
    21061 tttgttcttt ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc
    21121 cagcgtagca tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc
    21181 atttttcact tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg
    21241 taaatgtgca taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga
    21301 gttttcaatg acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat
    21361 gacacaatca gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt
    21421 gtctggaccg atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac
    21481 gccattgacc atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa
    21541 cgcgatccga tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac
    21601 tgtatggcct tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc
    21661 attgtttgtt ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt
    21721 tcctgtattg atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat
    21781 tttttctacg atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc
    21841 ttgtgccgtc aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt
    21901 cagcgtttca gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt
    21961 tccttccaag aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg
    22021 taacgcatat ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac
    22081 gatcgtaatc acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat
    22141 aggtttacca gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt
    22201 ccctgccgcc aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt
    22261 gctgatttca gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta
    22321 tcccagcgtt ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac
    22381 cggtgctctc ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct
    22441 gttttttgaa atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt
    22501 aaacgttttt caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag
    22561 caatttttcc gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga
    22621 taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa
    22681 agccattttc acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt
    22741 attctgaaaa ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt
    22801 tgacataaag cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg
    22861 caaatacagt gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt
    22921 tgactgttcc gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca
    22981 aacttctctt agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga
    23041 cacttactgt tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat
    23101 tcagataata ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag
    23161 catcgatttt ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt
    23221 caggttctcc taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt
    23281 ctgatagacg ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag
    23341 ggatgaacaa tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt
    23401 ctttaaatgt tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat
    23461 aacgttcggc aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg
    23521 tcatgtcaac tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa
    23581 ttttatcgtt ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat
    23641 aagaaaatag ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt
    23701 ataaggcttc ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt
    23761 ttgatgatat ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga
    23821 ggtgtatcta gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa
    23881 acactgatag caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga
    23941 agaaccatga ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc
    24001 acaccagtaa tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg
    24061 gcggtatctt catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata
    24121 ccgatggcaa cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc
    24181 gaaccaaaca gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat
    24241 actaacgcta gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt
    24301 cggtagatcg ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc
    24361 gtaagtgtag gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga
    24421 gaaagcgtat cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg
    24481 gcgattccgg caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc
    24541 aacgccattg agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga
    24601 tatcttcatg atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc
    24661 ggagaagacg ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa
    24721 gaacaaataa gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc
    24781 cgccagacaa ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag
    24841 cacgctgtac atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat
    24901 atcttcctga acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa
    24961 cttgttgagg aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct
    25021 ttatcattcc ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg
    25081 ctgctccatt ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat
    25141 cttctagtac aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca
    25201 ccttacaacc tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt
    25261 tccatgacag aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa
    25321 ggattcaaca cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa
    25381 gaggcatttt cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc
    25441 tcagctaagc gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt
    25501 tttaagtcgt attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg
    25561 tttgttgcat cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata
    25621 taagcatcgg cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct
    25681 tcagcaatcg ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg
    25741 tattcatgtg aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat
    25801 tctaaacggt cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa
    25861 tcctttaacg aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca
    25921 tcggaaattt ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca
    25981 gggatcaaaa gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc
    26041 ggataaaaag tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt
    26101 ccacatgctg caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc
    26161 atttatatgc tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat
    26221 gtatcaaaat aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat
    26281 attcttgatt aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa
    26341 taaaaaactg aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct
    26401 tatattttat tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt
    26461 atatactctc tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc
    26521 actcatcaat gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac
    26581 tggatgccgc tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg
    26641 ataatcttgc tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg
    26701 cgtatctact tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac
    26761 aacccagcac tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa
    26821 tgccgtactt ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc
    26881 cagttcttct aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc
    26941 acagtctgta cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat
    27001 cttgacaccg gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt
    27061 gttccgttca tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt
    27121 tatttcttca aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc
    27181 atgataccca tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc
    27241 tttttcaatc aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc
    27301 atagggaaaa ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca
    27361 acagctttca cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc
    27421 tgtgagcact tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag
    27481 tagctgcatg gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc
    27541 ccaatgatcg acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt
    27601 accataatta tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc
    27661 aacattagct aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac
    27721 aataaacaga ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg
    27781 caacaaatca tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac
    27841 tttttgatct ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg
    27901 tcccacagag aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac
    27961 agcttgatgt aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat
    28021 caaacgttga tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac
    28081 aatccctgct tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta
    28141 gctttctgat atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa
    28201 ctgattcgtt gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca
    28261 tttgtttcct ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata
    28321 aaaataactg taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc
    28381 caataaacgg tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc
    28441 taaatattga taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga
    28501 gaaccacaca tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa
    28561 aaagcgtccg ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct
    28621 agcatcaaag attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct
    28681 ggataaccac acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt
    28741 acgtgtgatt gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt
    28801 tttgactttt actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt
    28861 cactgtctga tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat
    28921 tgcagacaga ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc
    28981 acttacgagc acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa
    29041 tcgattgatt ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag
    29101 aacataataa aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga
    29161 caattgactc attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat
    29221 cgtcagcatt cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat
    29281 atctattctc atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc
    29341 aaaggatgcc agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt
    29401 tctctttaga taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca
    29461 gatccaatag cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag
    29521 aaacgaccat acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa
    29581 aaccattttt ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac
    29641 accaccaaaa ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc
    29701 ttttctttcc atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata
    29761 caacaacatc ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg
    29821 gctttgttgt cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc
    29881 gacttgattt gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact
    29941 atgttccttt atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta
    30001 acttgtatac ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt
    30061 agacctgttc tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg
    30121 ataacatgaa agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt
    30181 tattaaaaaa aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa
    30241 atgcacaaaa atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg
    30301 catcacaatc agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg
    30361 aagaactaag agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat
    30421 taatagattt agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga
    30481 atgtcaatgt cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg
    30541 aggaacagct tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag
    30601 cagaatcaga tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca
    30661 tgctgatcga tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca
    30721 ctgagattgc tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa
    30781 atccatggga acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag
    30841 ttgccttaga agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg
    30901 agaaagatct tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca
    30961 aaaaaagaag agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca
    31021 taatcagttg cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt
    31081 tcttcttcga ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt
    31141 tcttctggct tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct
    31201 acaccggcga agctgttcga aatattttcc agacttctta aacgacggat gtagttttct
    31261 aatgattcac ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg
    31321 acagagataa cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact
    31381 actgggtttt ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca
    31441 atctcatggt caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg
    31501 atatcttcgc caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc
    31561 ttcaagacgt tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga
    31621 tctggatgaa gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata
    31681 cgttcatcca tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt
    31741 cgtccatctt gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg
    31801 aatccggaaa gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc
    31861 gtctcaagcg ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca
    31921 ttcggaaggg taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga
    31981 atcgctaatg ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca
    32041 gattctttta ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg
    32101 acatctcgtg cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct
    32161 tcaatcattt tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt
    32221 gcttcttctt tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc
    32281 agcgtttgtt cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct
    32341 ctcagctcac tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat
    32401 gcttcttttt ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat
    32461 gattgtgcac cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca
    32521 agaccgacaa ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc
    32581 atactatctt tttcattttt atcatctata cagcttctat gataaattat tctaaactcg
    32641 tcaatatgca tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt
    32701 gtaccccgta gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac
    32761 gatggttttt aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact
    32821 tttctattaa attattcttt gtagatgaac agatattttt attttatgta cccactcgta
    32881 taattaaata actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc
    32941 tactcttcag accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt
    33001 cttcttgtcc tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc
    33061 taactagagc agaaacttct gccatcattt caggatgatt agccatatag attttagcgt
    33121 tttcccgtcc ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa
    33181 caatgtcttt ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat
    33241 acatgacatc tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga
    33301 tctttgtacg gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt
    33361 ccaaacggat cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcg
    33421 caaacatcac gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt
    33481 tgttgatcga accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga
    33541 catgtgagtc acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat
    33601 cgactactac gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt
    33661 gttctcctgt gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg
    33721 catattgcgg atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt
    33781 gtacttctgc aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata
    33841 cttcgatgat acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag
    33901 agccacttgg aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc
    33961 ctttaccata gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat
    34021 ctgccaatac gataatcctc cttatattta caaatcagag caacatttca ttatccttat
    34081 catatctttt ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt
    34141 ttcttaaaag ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag
    34201 cagcacgatc tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag
    34261 cgatccatac tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga
    34321 aagctacact gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac
    34381 attctttgct gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag
    34441 cttttgtttg gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa
    34501 tgtcgcctaa tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct
    34561 gcttgttttc tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc
    34621 cataaaaata ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat
    34681 tccctgcctg aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg
    34741 catatggtgc gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca
    34801 gtcgcgattc accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag
    34861 gaaaacgttc ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag
    34921 gcggaccggg aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa
    34981 gccctgtacg atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt
    35041 ttttggtcat ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg
    35101 tattttgtat gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt
    35161 ctgtcggtcc taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa
    35221 tcaactcttc taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac
    35281 ctagatcagc tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta
    35341 aaagttctgt tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt
    35401 atacgctgta tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc
    35461 ttgtcacagc tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt
    35521 cacaatatac ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt
    35581 agacggcagg ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc
    35641 gctgtttttg atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag
    35701 catgatttga tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa
    35761 gataatagca accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac
    35821 ttctccatgt tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat
    35881 gatcgctact acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat
    35941 aactagcatt ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg
    36001 cgcacgggca atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc
    36061 acctactagc tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa
    36121 aggtaaacct acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt
    36181 atttggtaaa ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag
    36241 tttccaccga tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa
    36301 attgatcttt ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt
    36361 tttataccta caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa
    36421 gagtaaggtt cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt
    36481 ctccattcgc tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg
    36541 ctgtttccgg taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat
    36601 aggcattgtc tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta
    36661 caggcttggt agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag
    36721 ctattgccat tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt
    36781 tggtctgcgt gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg
    36841 aagaagctgt gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca
    36901 cgacaacgac aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa
    36961 agcgacttgg atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag
    37021 gctgcggacg ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta
    37081 aatggtctcc atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa
    37141 atttaccagg caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa
    37201 tttttgtaat ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta
    37261 atttttctcc gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc
    37321 gaagcatctg ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc
    37381 acagctcctt ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca
    37441 atatccaata aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac
    37501 ggcaggctca cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc
    37561 aaaaaccggc aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa
    37621 acgctgattt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    37681 gtccaaccaa gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat
    37741 gcagggcagc tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca
    37801 ataagccgaa atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    37861 gaagctgacc acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata
    37921 agttgattaa aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta
    37981 agaataccat aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta
    38041 agacttttcc tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct
    38101 cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga
    38161 tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa
    38221 tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat
    38281 cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt
    38341 tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac
    38401 gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta
    38461 gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat
    38521 agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa
    38581 atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt
    38641 cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc
    38701 gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc
    38761 ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac
    38821 tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta
    38881 tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga
    38941 ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca
    39001 gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa
    39061 aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat
    39121 cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct
    39181 tttctttttc accataggga ataaattcgt tatcgatcga accataattc gtactgaaca
    39241 aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca
    39301 gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt
    39361 cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct
    39421 ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc
    39481 agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga
    39541 tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt
    39601 cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg
    39661 ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa
    39721 gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat
    39781 aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga
    39841 taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata
    39901 ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt
    39961 aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg
    40021 atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg
    40081 tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa
    40141 agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat
    40201 agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata
    40261 tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca
    40321 ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt
    40381 tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt
    40441 gtctgatccg aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc
    40501 gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat
    40561 tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac catatttata
    40621 tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta
    40681 cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt
    40741 tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta
    40801 tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc
    40861 attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt
    40921 cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat
    40981 cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc
    41041 actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat
    41101 tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc
    41161 aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac
    41221 tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa
    41281 tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa
    41341 aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt
    41401 tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg
    41461 aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt
    41521 tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca
    41581 tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca
    41641 tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct
    41701 tctccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg
    41761 attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc
    41821 tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac
    41881 aaaaaagcaa acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta
    41941 aaaattcgat ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa
    42001 aagaattttc ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa
    42061 cgccgcggcg ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc
    42121 cgatacgacc ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct
    42181 ctactatcaa ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa
    42241 atgcaataaa aataaataag aagctcatta agatttttct aaattattcc tcattataaa
    42301 atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt
    42361 gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa
    42421 aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata
    42481 aaagatgctg aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt
    42541 ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt
    42601 tctgcttcct caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa
    42661 agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt
    42721 cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta
    42781 aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag
    42841 cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct
    42901 tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa
    42961 tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg
    43021 aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc
    43081 gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt
    43141 tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg
    43201 attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg
    43261 tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact
    43321 tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc
    43381 gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag
    43441 ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc
    43501 tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc
    43561 gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca
    43621 ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc
    43681 atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa
    43741 ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata
    43801 ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct
    43861 gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag
    43921 aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca
    43981 taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat
    44041 ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct
    44101 tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt
    44161 agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt
    44221 tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt
    44281 ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc
    44341 accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa
    44401 atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc
    44461 attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc
    44521 ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga
    44581 aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag
    44641 gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt
    44701 caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct
    44761 aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga
    44821 ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct
    44881 tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt
    44941 gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc
    45001 tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat
    45061 cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag
    45121 gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg
    45181 gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc
    45241 atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa
    45301 ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc
    45361 atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca
    45421 tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt
    45481 tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa
    45541 tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct
    45601 ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat
    45661 tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa
    45721 gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata
    45781 tgatccatga ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct
    45841 tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct
    45901 gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct
    45961 cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata
    46021 tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata
    46081 gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc
    46141 tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt
    46201 ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta
    46261 ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc
    46321 ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt
    46381 cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag
    46441 ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat
    46501 tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa
    46561 acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat
    46621 cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc
    46681 gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag
    46741 tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc
    46801 tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt
    46861 cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat
    46921 accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc
    46981 gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga
    47041 tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga
    47101 aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag
    47161 ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag
    47221 gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga
    47281 aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc
    47341 taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat
    47401 cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt
    47461 aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc
    47521 gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag
    47581 gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc
    47641 gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt
    47701 cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat
    47761 agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga
    47821 ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt
    47881 tttggatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca
    47941 tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac
    48001 cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat
    48061 gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc
    48121 aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt
    48181 ctttacgttc agattgacgg taagggccgt attctccagg attttcagga gattcatccc
    48241 aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac
    48301 gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca
    48361 aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg
    48421 catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa
    48481 cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac
    48541 aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg
    48601 gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg
    48661 attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg
    48721 gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca
    48781 accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact
    48841 actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca
    48901 ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg
    48961 gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg
    49021 atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca
    49081 tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg
    49141 atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca
    49201 gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat
    49261 ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt
    49321 tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc
    49381 gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta
    49441 ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat
    49501 ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt
    49561 tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag
    49621 aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt
    49681 tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg
    49741 atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt
    49801 attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg
    49861 attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag
    49921 ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca
    49981 gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt
    50041 gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct
    50101 gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact
    50161 gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg
    50221 aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt
    50281 gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa
    50341 ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt
    50401 gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca
    50461 aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct
    50521 ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt
    50581 tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc
    50641 gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga
    50701 ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg
    50761 cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt
    50821 ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct
    50881 ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc
    50941 agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt
    51001 gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt
    51061 tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta
    51121 ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta
    51181 ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt
    51241 atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt
    51301 ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct
    51361 tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat
    51421 aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc
    51481 ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag
    51541 gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg
    51601 cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt
    51661 ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat
    51721 tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct
    51781 aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct
    51841 tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca
    51901 agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac
    51961 gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa
    52021 aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc
    52081 ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag
    52141 tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac
    52201 gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga
    52261 atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa
    52321 gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt
    52381 tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag
    52441 atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg
    52501 cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta
    52561 caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac
    52621 aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg
    52681 cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa
    52741 ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat
    52801 gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat
    52861 gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca
    52921 acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag
    52981 tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga
    53041 gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa
    53101 caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta
    53161 cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc
    53221 cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt
    53281 gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac
    53341 ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc
    53401 tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga
    53461 aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt
    53521 gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt
    53581 gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga
    53641 acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt
    53701 agcagaagac acagattggg atactgaata tctagattat atccttgctg tcaaagtggt
    53761 cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac
    53821 gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc
    53881 tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga
    53941 gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac
    54001 ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa
    54061 cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa
    54121 tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt
    54181 tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca
    54241 acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt
    54301 gcacattagt tacatggttg cattatgctt attagcgaca atcaattact attctttttt
    54361 cgatcattca atcgcttata cgattacgct actgattgct gggatttttc acttatctta
    54421 taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg
    54481 ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa
    54541 agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa
    54601 atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg
    54661 aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc
    54721 cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct
    54781 agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg
    54841 atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc
    54901 ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa
    54961 aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc
    55021 ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc
    55081 aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt
    55141 ctcacaacct cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac
    55201 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg
    55261 attttacaac ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa
    55321 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg
    55381 attttacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    55441 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca
    55501 gctttttcac aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga
    55561 taaatgatac aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt
    55621 tcaccaatat gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag
    55681 cggacaaaac tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca
    55741 atcgtcgtca acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc
    55801 ggtacaagga ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag
    55861 acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg
    55921 aaaatcgaag caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat
    55981 tcagaatagc caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta
    56041 tataacaatg cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct
    56101 tcccaaaggc ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct
    56161 ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg
    56221 cccaagaccc caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac
    56281 atcatgattg ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta
    56341 gagacaaata aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata
    56401 ttcccagata atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat
    56461 gtttttttca aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg
    56521 actaacatgt aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca
    56581 gcaatcgttt ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta
    56641 gctgctacaa ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata
    56701 tctatttgat tctccaatat ttccatccct ttctctctat tagcatcata catgatttat
    56761 tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa
    56821 taagacgaga ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt
    56881 atccaaacca cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca
    56941 tcacagtcag atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt
    57001 ggacaaagac gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct
    57061 tgtagaggaa tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc
    57121 aaaaatctat gctttttcga cgctcgataa aacaacagct actggactgc tattcactag
    57181 agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct
    57241 agacaagaaa caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca
    57301 gcaggaactc caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg
    57361 gataaacaga acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc
    57421 aatacagttg gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca
    57481 ctatttttct agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc
    57541 aaataacagt gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg
    57601 ggccaacgat gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca
    57661 tataagcaga gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca
    57721 tacatgtcac cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc
    57781 tttctggatt tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga
    57841 catccaaaat gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat
    57901 gaagatcttg atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca
    57961 tcattgttgt aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta
    58021 aatttgtcac gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt
    58081 ttctgccctt ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga
    58141 gaagtaaaca aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt
    58201 gccatatcaa taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa
    58261 agcaaagcaa tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc
    58321 aaagtttcct gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac
    58381 tctgtttgtc tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt
    58441 tgctgatata cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg
    58501 atatgttcta ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta
    58561 cctaaagaaa actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg
    58621 acattttgtc ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa
    58681 cggatttgtt tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct
    58741 aatgttttcg cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac
    58801 aaccgatcca gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc
    58861 gcttgttttt tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac
    58921 tctgcttctc gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa
    58981 ttccccgaaa actctcggat ttctccgtct tttacttccc agattttcga tgctagctca
    59041 tctagaaaat agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct
    59101 tcaatcagtc gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc
    59161 ccttgttcat aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc
    59221 catttccgct ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt
    59281 aggtcctttg cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt
    59341 tggattttcc catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta
    59401 ccgacaccat tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca
    59461 atagatagtt ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa
    59521 ttttccatat gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag
    59581 agcagaaaaa tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata
    59641 actatgacta aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa
    59701 atggacatac tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg
    59761 ttattcaaaa gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt
    59821 attaggttac cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct
    59881 tttattcgaa catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta
    59941 ccgcttttcc tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg
    60001 cctctgccaa ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac
    60061 gcgcaactaa catgaattgc atagccgcta agctagaatc gactttgact acatcgttca
    60121 tttcttctct tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga
    60181 cttcttttgg catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt
    60241 aacattgcat atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat
    60301 tgaaacggat caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc
    60361 gccatggttt catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca
    60421 tttcttcgtg tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca
    60481 taattgttga aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt
    60541 atattgcaaa gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca
    60601 tcgctgaaag ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg
    60661 attttcttct gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt
    60721 ctctgacgcg aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct
    60781 tgatcaatct gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt
    60841 ctcatcatga tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg
    60901 attttttgat cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata
    60961 atttcctcct ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa
    61021 aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata
    61081 gagaaaagaa attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa
    61141 accaagatta ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt
    61201 acaccgccaa aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg
    61261 gatcagtatg cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt
    61321 gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat
    61381 gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc
    61441 tctttttaat aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa
    61501 tctctttcct tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat
    61561 agtcgataaa ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg
    61621 atttttgtta aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa
    61681 ggattgtata aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca
    61741 tattcccagc ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt
    61801 tctcctgtat gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca
    61861 tactgtgtca gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca
    61921 gtggcataac gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc
    61981 catgcaccgg aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg
    62041 ttatcttgca atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc
    62101 cattgcccat tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct
    62161 ttaatcccaa atagattgta attaggggca gaagccaaag tactgttccc ccagccactt
    62221 tcaacgatgg cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt
    62281 tgtgcagttg aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca
    62341 taaaaagcat agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc
    62401 aatgtttgcc catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga
    62461 gcttctgctg atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct
    62521 tcggttgaaa ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt
    62581 tcaattcctg tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca
    62641 gctagcactg ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa
    62701 ttctttgctt tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta
    62761 actaaaaaag taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata
    62821 aatttgcttt ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt
    62881 aaattgatgg tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta
    62941 tggattatac ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg
    63001 gcgtttctat cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt
    63061 tttgcgcttc atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag
    63121 tttgaacagc tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac
    63181 gtaatccttc catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag
    63241 gataaacaac atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt
    63301 ccatgattgg aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca
    63361 tattttgcca ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat
    63421 tattgtacaa tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga
    63481 cttgtgcaaa atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt
    63541 cttttattgc ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt
    63601 ctgctaatag cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca
    63661 ctttttctga taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata
    63721 ctcactatct cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt
    63781 tggcagggat tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg
    63841 ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga
    63901 tccaagcatt tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat
    63961 cggttagatg gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta
    64021 aacagatgcc acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta
    64081 tactaatatg tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta
    64141 ctttgtcact aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag
    64201 tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga
    64261 gcggatccgt tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt
    64321 tgctccctcc ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa
    64381 acatacttaa ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg
    64441 aaaaaatgga tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca
    64501 gccgagcagc aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt
    64561 tagaagaaga acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg
    64621 attctggtcg atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg
    64681 cgaacaattt aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa
    64741 ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata
    64801 tccagtttca tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat
    64861 tacttgattt tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac
    64921 tgccagccca agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa
    64981 cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag
    65041 tggacagtca actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa
    65101 catataactt attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag
    65161 ctctagatgg gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc
    65221 cattacaagc ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc
    65281 gtaaattttt agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt
    65341 tcagcttttt aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt
    65401 cttaatttgt tccttccttt tatttctgat aattccagta aattactata attataaaaa
    65461 aagaattgac aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc
    65521 atgtgactag atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt
    65581 ttttcgtgtc tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat
    65641 cttcaaaaag ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt
    65701 agatgacggt ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag
    65761 aaatgatttg atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca
    65821 aatcaagttg caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac
    65881 gatcattgat catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc
    65941 tttgaccgac cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa
    66001 caaactttta agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc
    66061 agtagagtat ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat
    66121 cgcaatccat attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga
    66181 agtgacgatt gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca
    66241 ttcagaacca ttttcactga attattcaag actggtgaat catttacgac tgcttctaca
    66301 acgaacccat gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg
    66361 aaaataccca gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata
    66421 ccagctcgcg atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag
    66481 aagcgcaatc gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat
    66541 ctacaacgaa tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta
    66601 ctagtaggga tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta
    66661 caagctggcg gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca
    66721 ctagggatgt cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa
    66781 gttccagtaa atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa
    66841 tcagttgatc catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg
    66901 attgcggcag ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc
    66961 tttagcggca agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca
    67021 gtgctactgc tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt
    67081 ttgagtttag gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct
    67141 acaggattgc atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat
    67201 attggtaaat ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga
    67261 tttttcccaa tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca
    67321 cgtccagaaa acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc
    67381 tttactggtg taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat
    67441 gtcttgcatg caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca
    67501 gcaggatttg cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt
    67561 gcgaatcagc cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt
    67621 ggctttaact ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat
    67681 ctttcagatg acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc
    67741 aaaatctacg ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca
    67801 cgccttcgtt tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact
    67861 ggcgtacctg gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa
    67921 gtacaattcg ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca
    67981 caaacaaaca ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt
    68041 tacgctatcg caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg
    68101 gaaaaaatga tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca
    68161 atcgctggta cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat
    68221 gctggtatcg aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca
    68281 ttcactttat atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt
    68341 gatcttgccg ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat
    68401 ggcgacaaag tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa
    68461 atcggctctg tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa
    68521 acaaacagga gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt
    68581 tctcctgcca actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt
    68641 tatttgactc ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt
    68701 gttcaaaaac cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt
    68761 tcacaaaatt tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt
    68821 attcctgatc caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata
    68881 ctttttctaa atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg
    68941 ctaaaaacat gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg
    69001 ctgcatcact tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg
    69061 ctaattcttc atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt
    69121 ctactagtac ttttgctgca gcttctgcat attcacgttt caatacccaa cctgtttttc
    69181 catttcctgc agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt
    69241 cattctctac ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca
    69301 ttttttcagt gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa
    69361 aacttgtata agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat
    69421 gttcggcttg acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca
    69481 acgcttcttt cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt
    69541 ctttcagtgc ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa
    69601 ccatcttttt taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta
    69661 aataatgcat atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt
    69721 tataaattac acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa
    69781 ggatgacaac tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat
    69841 attgaaatgt ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa
    69901 acaaatgcag caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt
    69961 acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta
    70021 gatgtctatc gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat
    70081 ccaaattgcg tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg
    70141 cagaaaaagg cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata
    70201 gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa
    70261 ttctctctac tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct
    70321 ccgggggaca aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct
    70381 gaagtcatga cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa
    70441 aaatgcaaaa tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc
    70501 tactttcacg acctctatat tatcatggtt tattgactac cggtatccag atttctccca
    70561 tatatttccc gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt
    70621 aatcttctac ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt
    70681 cttcaggcga ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga
    70741 ttcgggtagt gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc
    70801 cgttataggg ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat
    70861 ctattgttcc attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga
    70921 caaaatccgt attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt
    70981 tttcttcgat cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt
    71041 acataaaatt aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt
    71101 ttaactattt ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct
    71161 ctccccattt tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa
    71221 gccattctgc atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat
    71281 taggaaaaat ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat
    71341 tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg
    71401 ctccatcaaa tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat
    71461 gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga
    71521 ctccttcaaa tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct
    71581 tttcgatacg aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg
    71641 gaaaagattt ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac
    71701 gaaaagctct tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca
    71761 ctcttgatcc atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt
    71821 ccattagcga catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc
    71881 ctgtattttc ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat
    71941 cgatcaattg gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca
    72001 tattatcaaa gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa
    72061 atgttgtatg gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta
    72121 tctgcctatc tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta
    72181 aacaaagcgg taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca
    72241 aagcaaattg ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca
    72301 tcatacctct tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg
    72361 tcagataaat aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt
    72421 ctcacattct ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat
    72481 tatcaaatat gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat
    72541 aagtgtttca tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt
    72601 tttggaacgc ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag
    72661 caagtacttg tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga
    72721 tgaaaagacc aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca
    72781 catggctgct gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact
    72841 ttccgaactc tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac
    72901 agagattctt atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca
    72961 tgcaataaat tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa
    73021 gaaaaacaaa aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga
    73081 agcagttgaa tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga
    73141 agtgattgct actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct
    73201 gatagaggac tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa
    73261 aaggcaaatg ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa
    73321 acgctactcc ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa
    73381 gaaaaaaata gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata
    73441 gcaagtaaac gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa
    73501 aacacgaatc atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat
    73561 ttgactatta ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt
    73621 agattttccg gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt
    73681 ccactgcatc tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact
    73741 ctaatcttta aagataaata ttaaaataac gattaataat attaacagtc tttatttttc
    73801 accaccaaaa acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc
    73861 ttgtgaaaaa taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt
    73921 atcaaaaaat atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg
    73981 ttaatttttg ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt
    74041 gttcgtcaat tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt
    74101 gtaaaacagc tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg
    74161 tagtccaagg aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg
    74221 ctactgtcaa agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata
    74281 caataggcaa tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata
    74341 atttagcaaa gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag
    74401 caatcgtatt tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa
    74461 tatgtggaat ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca
    74521 aaaacggatc taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata
    74581 gattagcaat agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg
    74641 gctctgtaac tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta
    74701 taaatgacaa taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag
    74761 actttaaaac agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag
    74821 aaagtttaat aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg
    74881 aagctcctgt ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt
    74941 caggagtaaa catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata
    75001 ccgctgcaat atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta
    75061 caactaataa tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta
    75121 acgtggtcaa tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca
    75181 ctgaattaat aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg
    75241 catctcgcaa agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt
    75301 tttcaaatac aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt
    75361 gtaatcgatt actttttcca cccaaataat acatcactta tcaaaaaaag ta
//
LOCUS       DADQRX010000004        61712 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.004, whole genome shotgun sequence.
ACCESSION   DADQRX010000004 DADQRX010000000
VERSION     DADQRX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.004"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..509)
                     /locus_tag="J8V14_000247"
     CDS             complement(132..509)
                     /locus_tag="J8V14_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920385.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="J8V14_000248"
     CDS             765..1493
                     /locus_tag="J8V14_000248"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBA0920386.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="J8V14_000249"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="J8V14_000249"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBA0920387.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="J8V14_000250"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="J8V14_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBA0920388.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="J8V14_000251"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="J8V14_000251"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBA0920389.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="J8V14_000252"
     CDS             4628..6067
                     /locus_tag="J8V14_000252"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBA0920390.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6099..7142
                     /locus_tag="J8V14_000253"
     CDS             6099..7142
                     /locus_tag="J8V14_000253"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBA0920391.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="J8V14_000254"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="J8V14_000254"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBA0920392.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="J8V14_000255"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="J8V14_000255"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBA0920393.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="J8V14_000256"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="J8V14_000256"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBA0920394.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="J8V14_000257"
     CDS             10661..11575
                     /locus_tag="J8V14_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBA0920395.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="J8V14_000258"
     CDS             complement(11708..13273)
                     /locus_tag="J8V14_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBA0920396.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="J8V14_000259"
     CDS             complement(13266..14717)
                     /locus_tag="J8V14_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA0920397.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="J8V14_000260"
     CDS             complement(14731..15747)
                     /locus_tag="J8V14_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0920398.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="J8V14_000261"
     CDS             15967..17055
                     /locus_tag="J8V14_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA0920399.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="J8V14_000262"
     CDS             17109..18635
                     /locus_tag="J8V14_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA0920400.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="J8V14_000263"
     CDS             18637..19842
                     /locus_tag="J8V14_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0920401.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="J8V14_000264"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="J8V14_000264"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBA0920402.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="J8V14_000265"
     CDS             21340..22764
                     /locus_tag="J8V14_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA0920403.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="J8V14_000266"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="J8V14_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBA0920404.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="J8V14_000267"
     CDS             24337..25314
                     /locus_tag="J8V14_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBA0920405.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="J8V14_000268"
     CDS             25339..26982
                     /locus_tag="J8V14_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBA0920406.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="J8V14_000269"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="J8V14_000269"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA0920407.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="J8V14_000270"
     CDS             28558..28833
                     /locus_tag="J8V14_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBA0920408.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="J8V14_000271"
     CDS             28839..29606
                     /locus_tag="J8V14_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBA0920409.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="J8V14_000272"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="J8V14_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBA0920410.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="J8V14_000273"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="J8V14_000273"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBA0920411.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="J8V14_000274"
     CDS             33750..34052
                     /locus_tag="J8V14_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBA0920412.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="J8V14_000275"
     CDS             34055..35218
                     /locus_tag="J8V14_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBA0920413.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="J8V14_000276"
     CDS             35244..38672
                     /locus_tag="J8V14_000276"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBA0920414.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="J8V14_000277"
     CDS             38693..39817
                     /locus_tag="J8V14_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920415.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39807..40244
                     /locus_tag="J8V14_000278"
     CDS             39807..40244
                     /locus_tag="J8V14_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBA0920416.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="J8V14_000279"
     CDS             40228..40566
                     /locus_tag="J8V14_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBA0920417.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="J8V14_000280"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="J8V14_000280"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBA0920418.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="J8V14_000281"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="J8V14_000281"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBA0920419.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="J8V14_000282"
     CDS             41723..42766
                     /locus_tag="J8V14_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBA0920420.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="J8V14_000283"
     CDS             42925..43653
                     /locus_tag="J8V14_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBA0920421.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="J8V14_000284"
     CDS             43769..44014
                     /locus_tag="J8V14_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBA0920422.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="J8V14_000285"
     CDS             44011..44229
                     /locus_tag="J8V14_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA0920423.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="J8V14_000286"
     CDS             44365..44904
                     /locus_tag="J8V14_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBA0920424.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="J8V14_000287"
     CDS             44897..47170
                     /locus_tag="J8V14_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBA0920425.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="J8V14_000288"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="J8V14_000288"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBA0920426.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /locus_tag="J8V14_000289"
     CDS             complement(48343..48597)
                     /locus_tag="J8V14_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBA0920427.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="J8V14_000290"
     CDS             complement(48997..49266)
                     /locus_tag="J8V14_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920428.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49736..50191)
                     /locus_tag="J8V14_000291"
     CDS             complement(49736..50191)
                     /locus_tag="J8V14_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBA0920429.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="J8V14_000292"
     CDS             complement(50329..52257)
                     /locus_tag="J8V14_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBA0920430.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="J8V14_000293"
     CDS             complement(52454..53287)
                     /locus_tag="J8V14_000293"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA0920431.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="J8V14_000294"
     CDS             complement(53479..54183)
                     /locus_tag="J8V14_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA0920432.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="J8V14_000295"
     CDS             complement(54206..54832)
                     /locus_tag="J8V14_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA0920433.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="J8V14_000296"
     CDS             55158..57533
                     /locus_tag="J8V14_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBA0920434.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="J8V14_000297"
     CDS             complement(57557..58048)
                     /locus_tag="J8V14_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBA0920435.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="J8V14_000298"
     CDS             58235..59155
                     /locus_tag="J8V14_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBA0920436.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="J8V14_000299"
     CDS             59309..59653
                     /locus_tag="J8V14_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBA0920437.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="J8V14_000300"
     CDS             complement(59843..60343)
                     /locus_tag="J8V14_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920438.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="J8V14_000301"
     tRNA            60871..60954
                     /locus_tag="J8V14_000301"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="J8V14_000302"
     CDS             complement(61278..>61712)
                     /locus_tag="J8V14_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBA0920439.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20240 a        10663 c        13217 g        17592 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 ataggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DADQRX010000005        60125 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.005, whole genome shotgun sequence.
ACCESSION   DADQRX010000005 DADQRX010000000
VERSION     DADQRX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 60125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.005"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            527..721
                     /locus_tag="J8V14_000303"
     CDS             527..721
                     /locus_tag="J8V14_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920440.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="J8V14_000304"
                     /pseudo
     CDS             912..1161
                     /locus_tag="J8V14_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1405..2235)
                     /locus_tag="J8V14_000305"
     CDS             complement(1405..2235)
                     /locus_tag="J8V14_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBA0920441.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2443..3777
                     /locus_tag="J8V14_000306"
     CDS             2443..3777
                     /locus_tag="J8V14_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0920442.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3818..4729
                     /locus_tag="J8V14_000307"
     CDS             3818..4729
                     /locus_tag="J8V14_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0920443.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4716..5579
                     /locus_tag="J8V14_000308"
     CDS             4716..5579
                     /locus_tag="J8V14_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0920444.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            5576..6192
                     /locus_tag="J8V14_000309"
                     /pseudo
     CDS             5576..6192
                     /locus_tag="J8V14_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6194..6712
                     /locus_tag="J8V14_000310"
     CDS             6194..6712
                     /locus_tag="J8V14_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBA0920445.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            7010..7468
                     /locus_tag="J8V14_000311"
     CDS             7010..7468
                     /locus_tag="J8V14_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0920446.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            7481..11518
                     /locus_tag="J8V14_000312"
     CDS             7481..11518
                     /locus_tag="J8V14_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA0920447.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            11518..12831
                     /locus_tag="J8V14_000313"
     CDS             11518..12831
                     /locus_tag="J8V14_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBA0920448.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            12824..14521
                     /locus_tag="J8V14_000314"
     CDS             12824..14521
                     /locus_tag="J8V14_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBA0920449.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            14595..15488
                     /locus_tag="J8V14_000315"
     CDS             14595..15488
                     /locus_tag="J8V14_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBA0920450.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            15672..16346
                     /locus_tag="J8V14_000316"
     CDS             15672..16346
                     /locus_tag="J8V14_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920451.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            16364..17389
                     /locus_tag="J8V14_000317"
     CDS             16364..17389
                     /locus_tag="J8V14_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0920452.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            17478..18344
                     /locus_tag="J8V14_000318"
     CDS             17478..18344
                     /locus_tag="J8V14_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBA0920453.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            18381..19757
                     /locus_tag="J8V14_000319"
     CDS             18381..19757
                     /locus_tag="J8V14_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0920454.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(19858..21054)
                     /locus_tag="J8V14_000320"
     CDS             complement(19858..21054)
                     /locus_tag="J8V14_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBA0920455.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(21178..21840)
                     /locus_tag="J8V14_000321"
     CDS             complement(21178..21840)
                     /locus_tag="J8V14_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HBA0920456.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            22068..22631
                     /locus_tag="J8V14_000322"
     CDS             22068..22631
                     /locus_tag="J8V14_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBA0920457.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            22644..22928
                     /locus_tag="J8V14_000323"
     CDS             22644..22928
                     /locus_tag="J8V14_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920458.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            22947..23639
                     /locus_tag="J8V14_000324"
     CDS             22947..23639
                     /locus_tag="J8V14_000324"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBA0920459.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            23651..24196
                     /locus_tag="J8V14_000325"
     CDS             23651..24196
                     /locus_tag="J8V14_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA0920460.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            24467..27745
                     /locus_tag="J8V14_000326"
     CDS             24467..27745
                     /locus_tag="J8V14_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBA0920461.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(28286..30193)
                     /locus_tag="J8V14_000327"
     CDS             complement(28286..30193)
                     /locus_tag="J8V14_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBA0920462.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            30323..31198
                     /locus_tag="J8V14_000328"
     CDS             30323..31198
                     /locus_tag="J8V14_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBA0920463.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            31278..32252
                     /locus_tag="J8V14_000329"
     CDS             31278..32252
                     /locus_tag="J8V14_000329"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBA0920464.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            32441..33577
                     /locus_tag="J8V14_000330"
     CDS             32441..33577
                     /locus_tag="J8V14_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBA0920465.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            33714..34058
                     /locus_tag="J8V14_000331"
     CDS             33714..34058
                     /locus_tag="J8V14_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBA0920466.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            34329..35615
                     /gene="efmA"
                     /locus_tag="J8V14_000332"
     CDS             34329..35615
                     /gene="efmA"
                     /locus_tag="J8V14_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBA0920467.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(35753..36352)
                     /locus_tag="J8V14_000333"
     CDS             complement(35753..36352)
                     /locus_tag="J8V14_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HBA0920468.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            36756..37877
                     /gene="mnmA"
                     /locus_tag="J8V14_000334"
     CDS             36756..37877
                     /gene="mnmA"
                     /locus_tag="J8V14_000334"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBA0920469.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38123..39544
                     /gene="arcD"
                     /locus_tag="J8V14_000335"
     CDS             38123..39544
                     /gene="arcD"
                     /locus_tag="J8V14_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBA0920470.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            39558..40676
                     /locus_tag="J8V14_000336"
     CDS             39558..40676
                     /locus_tag="J8V14_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBA0920471.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(40736..42028)
                     /locus_tag="J8V14_000337"
     CDS             complement(40736..42028)
                     /locus_tag="J8V14_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBA0920472.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            42178..42738
                     /locus_tag="J8V14_000338"
     CDS             42178..42738
                     /locus_tag="J8V14_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0920473.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            42867..43244
                     /locus_tag="J8V14_000339"
     CDS             42867..43244
                     /locus_tag="J8V14_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBA0920474.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            43177..45249
                     /locus_tag="J8V14_000340"
     CDS             43177..45249
                     /locus_tag="J8V14_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBA0920475.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(45460..46407)
                     /locus_tag="J8V14_000341"
     CDS             complement(45460..46407)
                     /locus_tag="J8V14_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA0920476.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            46653..47117
                     /locus_tag="J8V14_000342"
     CDS             46653..47117
                     /locus_tag="J8V14_000342"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBA0920477.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(47179..48222)
                     /locus_tag="J8V14_000343"
     CDS             complement(47179..48222)
                     /locus_tag="J8V14_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBA0920478.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            48573..49436
                     /locus_tag="J8V14_000344"
     CDS             48573..49436
                     /locus_tag="J8V14_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBA0920479.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(49565..51187)
                     /locus_tag="J8V14_000345"
     CDS             complement(49565..51187)
                     /locus_tag="J8V14_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0920480.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            51436..51957
                     /locus_tag="J8V14_000346"
     CDS             51436..51957
                     /locus_tag="J8V14_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920481.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(52143..53873)
                     /gene="spxB"
                     /locus_tag="J8V14_000347"
     CDS             complement(52143..53873)
                     /gene="spxB"
                     /locus_tag="J8V14_000347"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBA0920482.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            54038..54856
                     /locus_tag="J8V14_000348"
     CDS             54038..54856
                     /locus_tag="J8V14_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBA0920483.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(54959..55156)
                     /locus_tag="J8V14_000349"
     CDS             complement(54959..55156)
                     /locus_tag="J8V14_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920484.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            55325..55903
                     /locus_tag="J8V14_000350"
     CDS             55325..55903
                     /locus_tag="J8V14_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBA0920485.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(56948..57451)
                     /locus_tag="J8V14_000351"
     CDS             complement(56948..57451)
                     /locus_tag="J8V14_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBA0920486.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            57810..58835
                     /locus_tag="J8V14_000352"
     CDS             57810..58835
                     /locus_tag="J8V14_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA0920487.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            59158..60036
                     /locus_tag="J8V14_000353"
     CDS             59158..60036
                     /locus_tag="J8V14_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920488.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT        19141 a        10625 c        12623 g        17736 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa
     2341 taattttttt agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat
     2401 attaaaaccg gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt
     2461 acagggttgc tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca
     2521 agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat
     2581 attgatttct ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt
     2641 gaggatttca atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt
     2701 gacatctgga ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta
     2761 caggacataa attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa
     2821 tatgtagaaa aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat
     2881 gaaggtgatc cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa
     2941 aagatattta aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta
     3001 gaagaaacag cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt
     3061 tatccattat ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt
     3121 tggtttgaca aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag
     3181 tggatccaag actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa
     3241 tttgggtctg gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat
     3301 atcaattatt actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg
     3361 cagctcccag aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg
     3421 gaagtaccat acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct
     3481 tctccagaag tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca
     3541 gccaatgaga atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa
     3601 atggcggacg aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat
     3661 tacacgacat tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa
     3721 gaaggattgg cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc
     3781 atttttgact gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa
     3841 gaaaaaagaa gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt
     3901 atttatgcta gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac
     3961 atccaagatg aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt
     4021 attttggaaa tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac
     4081 gattttcagt gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt
     4141 ccgtacagct ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat
     4201 gcagctgctt tctccatctg caggattgat caacacattt ctgggctggt tccatatccc
     4261 tggaccagct tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat
     4321 atggagttct ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc
     4381 tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac
     4441 tttgccatta atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt
     4501 ccaaattttt caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc
     4561 tttgcttttt tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta
     4621 tgcgatggct atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt
     4681 gaagcttgca cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc
     4741 aaaaagacca gtgtgaatcg gcaaacaaaa aaatactaag ttatgtcctc atgacagtaa
     4801 taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg aaaccaatgg
     4861 aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg gaaaactatc
     4921 tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta ttcatcactg
     4981 tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga tttgcgaagc
     5041 ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg atgatcccgg
     5101 ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg gtaggtactt
     5161 atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt ctgatgcgtc
     5221 aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac ggagcgaatc
     5281 atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg attaccattg
     5341 cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta tatatccaag
     5401 atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa gcaacgacac
     5461 aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt ttgctgttct
     5521 tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca aaaggatgat
     5581 gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg aatggattcc
     5641 acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt tcacattagg
     5701 aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaatga agagaaaaag
     5761 ctcggttcac tattctggaa aaagtttcgt gagaacttct ctctttatgg aaagcaagat
     5821 agtttagcaa gtgtttattt tcttcttcta ttgatcgatt atcagatttt ccgctattgg
     5881 ggtgggggaa ttggctatac attgatgtat actacgttgt ttttgcttct attaagcgcg
     5941 cttctcttta gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc ttggattact
     6001 gcatttttct tgttttttaa tgatttcaaa agcgcgcttt tctatttggc aggcacgttg
     6061 atccttcttg tcgcattctg gtttgcagga ccaatctatt ttgctttatt aggtttcagt
     6121 ttcttgtttt attttcaagt cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa
     6181 aaggaggaat aacatgttgc gtttgattgc taaaatcgat gatagtatta tcaacaagtt
     6241 cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta tcagtaatat
     6301 ctctaatggt ggttttattt ggttgctcct tgctgctgtt ttagtcttga tacctgatac
     6361 tagagaatat ggtctagcca ttcttatcgg cagtttgatc tatttttttg ttggtttgct
     6421 ttttatcaaa aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct
     6481 ttacacaagt aaaccgaaga cttcttcttt tccttcagga catacagcgc tagccttttt
     6541 atcagtagct gcgttgttcg aattaagtcc agttgcagga atattagcct ttttatttgc
     6601 ttgtttgatc gcttattcga ggattttttt acttttacat tatcctactg acattttagg
     6661 aggagctatt ttaggaagtg ttattggcat cctatgcgct gccgttatct gaattaattg
     6721 tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa
     6781 agcgtagttg tttattcatt tgtcttaata acttaagttt tgttaaatga gaacgttata
     6841 aaattttata atttaatttt aataataaat gttgtataat tggtgttttt taactaaaaa
     6901 ggatatcatt tgatttaaaa ttaaaattaa attaaattat aattagatgg gcattgtttt
     6961 gttgattgtt gtgaaagaag acaaatattt ttgaaaggag tataaggatg tgaaagggtt
     7021 aaaattgaag cttttgcagt tagtaactgt cagtttgcta tttggaccat atttccagtc
     7081 agtctatcat atgacgaccc tggatgtagc tgctgagtca acagaaacaa aggaggatga
     7141 ggtattctta gatgaggact atgcaaaaat tgaagggaag tatattgaaa aagaagaaac
     7201 ccttgaatgg catctggctt ttgaaaaaaa ggaaagtgcc aatgaaggta gaatacgttt
     7261 agcaatcgat gcggcagccg ccgggattgg taccgtaaaa aacgttcgtg gaacaggtct
     7321 atcctcctat gatgaagatg gagaagacct aaaagtcgag acgatcgatg gtcaagaatg
     7381 gtatgtcgga aaagtttatt caaaagaagc agaaaccggc acattgattt ttgaaacaga
     7441 aaagatcccc gatgtgaacg aagggtaact acctcttcaa gtggtggttg atgaaagaat
     7501 agataaccaa gaattacaag aaaatgaaac tgatacagaa gattccgctg aaattcaacg
     7561 tagcgaagaa aatgagatgg aaaagctgga cgaaccaatc gtacccagcg gtcaagcact
     7621 ccagcgcatt caacgccagt tattcaacgg tattttggag cgtcttgggc caacagctac
     7681 gcaagacccc tatgaatata tctatcctag cggagaagtt gaaaacgaac agcatcgcta
     7741 cccgaagttt acaacgaatg actatacaaa agtgttagga aattggacac aaagcaatga
     7801 acgctatcaa ggagacaaag gctcgccata tgacggagag aacttgatcg atgatagtga
     7861 ttatacaaac gggggagcga actggcgaaa ttacgactat gctactaata atggatcaac
     7921 tgcaggcgaa gaagatagaa acgttccagc tcaaaccgtt cagctttggg gaaatgaacg
     7981 gaattttgaa aacagttact tggattataa cggggcgtac atcaaaaaat gggtagaacc
     8041 tgttctgcca agcagcacag catctgatct acatcctgaa gatgcaacaa ccctttacaa
     8101 tgtctatcta gatgtcatcg ggggcgagaa aaaggaaatc agtccaatcg atatcgtttt
     8161 tgttctagat aagtcagcga gtatgagtga attaaccgct gggaccaata gtcaaactaa
     8221 aaatgctgcg ttgattgagg cagtaaacga aatgagtaaa gatctgctct ctgacccgag
     8281 tctggatatt cgaatcggta tggtgaattt ttaccataat agtacagcta tcaataacca
     8341 tgagcaaatc agttcggaca tctttccatt gacaaatgat atcaatcgtt tgacgggaag
     8401 tgagaataca gcattaaatc gtacaccaat cggaggaact ccgcttacac tagggcttaa
     8461 aaatggctat gaaacattgt ataaagacaa tggcggagaa aatagaaatc cagaaaaaat
     8521 cctaatcgtt gtgggagatg ggacaccgac cttcagttat gcaccgattc agtcaagtta
     8581 tcgtacgagc actaatggag cttggagcaa ctggactgtg atggaagata agattgctga
     8641 agataatgac gttttgttca gaaattttga ggagttttct gggaatactt ctaatgcagg
     8701 gtttactcat ccagttacct atgcatctga ctttaaccgt ccagaagatg aagtaaatgt
     8761 acactatcgt tatggagagg taaaagaagg agataataaa gcgactcact gggttggtga
     8821 tggttcttct aataacaata cgaatggcag tcctacttcg caagaaaagt catctgcgat
     8881 caacacagtt gcttatcatc attggctgaa aaacaagtat caagagaacc cgccaagtat
     8941 cttctccatt ggtttaggaa tcgatggaaa cgttagcggg agacaacgac tagatgctat
     9001 tggacgaaat gtattgaaaa atattgctga tttagaagaa gatggcgtaa caccgcgata
     9061 ttacaatgcg aacaacaaga atgacattgt tacagccttg gaggatatct cttctacctt
     9121 caagagaacg atccagcaag ccacacttta tgatgaaact ggttttaatg tctcaccatt
     9181 tggagctggc agatcggcag atattcagtt ttaccatcta gatccaggag aaaacggcat
     9241 gaagtaccaa gagccagaaa tatgggatga agaacgtcat ggacaagccc caccagaagt
     9301 agtggcgact ccgacaacgt atgtcggacg ggaaaaccaa gcttataaat tttctccgat
     9361 ttctttaggt gaaggggaaa tggtcaggat caagtaccaa gtaaaacttg atgcgggagc
     9421 tcaagacgga aatttttgca cagtgaacaa tatggcctat attcagaacc tggaggatta
     9481 tgagaatgat atcactactg gacggatgta tttcccatct tcttccattc gttacgaaca
     9541 taaaggccgt aatttccaag tcagcaagaa gggtcaaaat ggcgagccgt tggctggagt
     9601 cgaatttgca ttatatgacc aaaatccaga gagtggggaa gccgaaccaa tcgaaacaag
     9661 taaaactacc acggacggaa tagcgatctt tgaaacgaag atgccgattt taggagatga
     9721 aactggagca gtcagcgaca tattttggat cagagagatc gctggaccac cacgttatca
     9781 gttagtagat gagacgtatc aattccaaat caaaaaatat gcagccaacg aagatgagat
     9841 gttaggtcac tacgagcttg tagatgaata tacatctggt tcgagagtcg gcggaccgga
     9901 aggtaaagat ccgaattcct attatgaagg agaatatcac aagctttctg ttgtacaaga
     9961 gcctaacata gaaggcacag agtatgatga gttgtatgtc aaactggaaa tgcgaaatga
    10021 attcaagcca atccacatga atatcaataa actaattgaa ggcagtaata tgccaatcaa
    10081 tggcgcagag tttgaacttt atgaaacaga tggtaacgga ctggaaatcg gatatccagt
    10141 agcgaaaggg ctttctggtg tgaacgaagt agaggaagag aaagggagac tcacgttcta
    10201 ttcagtagat gaagacggga aatatgtaat cgtggatggt gagaaagtta ttcatccaat
    10261 cggagagcca gattcatttg tttataagga tagtgaagaa actaccaact atgcagaata
    10321 caaaatcatt gagagccaga acccagaagg tttcaaagaa cctgatgaaa atgacacatg
    10381 gatcttgcga ctttacgata atgctatagg gactatccaa ctcaagcaaa caggtgaaga
    10441 aaattggcaa tatctcacgt atacaacaga tgaagaggat cgtttatggg tagcattcac
    10501 tgcttacaat acgtttgatt atcgggagat ccgagttaga aaattagacc atgaaggcca
    10561 tacattagat ggtgcaggtt tcgatatcag gaaagtgggg gattcttcct tcccgctgta
    10621 tcgagcatac actggtaatc cgttagaacc agaagaatcg aaacgtttgc ctgggatcgg
    10681 atacttctat tctcgtgccg taggagtaga aaagtatgtc gatttaaatt acgaccaacc
    10741 tctgcatctg tctattggtg aatatgaggt ctcggaatct gtcgcaccaa atgggtatcg
    10801 gctatctgat caagtgttcc agtttgcact gaatgaagaa ggtaagttta tcattgaaga
    10861 tacggtcatc gaagacgaaa ctgctccctt gccggaaggc tatgaaatca tcgatggtgt
    10921 actacaagta accttagaag atgaactagc gccaatcgat ttggaacttt tgaaaattga
    10981 cagtatgaat agccgacgtc ttgaaggcgc agaattttca attgagaaac aaaatagtgc
    11041 aggagagtat gaaatcatcg agggaggctt ggcaccagat ccaaacgatt cttccctatt
    11101 caatgcttca gacttgttgg aaggggtcta tcggatcaag gaagtgaagt cacctgacag
    11161 ctatagaaaa cttccaggtt actttatttt agaaattagt tatcgagaag aacctgacat
    11221 agaaaatgat cgggtagttc caggaaaaga agcgggtaca ctaaaagttg agatttcgta
    11281 ttatccaaac gaagaagcag agacgcctga tctacagcaa gagttagaat atgaactttt
    11341 ggagacaaat cgaatcagat tacagcttag tgtcgggaac gatcctgaaa atccgttgcc
    11401 tgctacagga ggatccggac gattattcta tatcctgctt gctgcattct tcatggcgct
    11461 tgcaggagca gcctatggtt tgtttgtcag acaatctaaa aaggagggag caaggtaatg
    11521 aaaaagctgt tgtgtttgat gcttcaatta cttgttttac taccgcttgc agcagtcctt
    11581 cttccacatg cgatgtcggc agaagaatca ggggaagatg tcgtggaatt cgtgttgcat
    11641 aaacggatga ttcgagatat cgattataac gatcaatttg aataccatga aaatgatggc
    11701 ttggctatct ctgaggaagc aggggaaaca gcagatatca tttcacagac ggtgccacta
    11761 aatggagcga catttgccat ttacgacatg accgattatt atcatgaaaa aaaagctgct
    11821 gaaaacatga ctcctaaaga attcgtccag caaatcgctg aaacgaacag aaacaatatc
    11881 cgtcagctaa tcacagaaga aaggcttgtc caaataggga cttcggtaac aactgggaaa
    11941 gatacggttg gcggattagg agacgggatt gcacgtatca aagcacctaa gaaaaaccaa
    12001 gatcgggatg cagtttattt gattttcgaa gaatcgattg atccagaagt tggattggat
    12061 gttgatatcg atcagacagc tatcccaatt gctgctattt tgccaattta tcacccgaca
    12121 gaatcaaccg aggaactgca agagattcat atctatccta aaaatgtcgg ttatctaaga
    12181 gatccatact tcttcaagta tggacgtcaa agaggaacaa ctgaaaaagg aaaaccatta
    12241 gaaggtgctg tttttgctct atatcagatg atagaagggg ggaaatatta tttagatatg
    12301 gctcctgcta atgacttgaa aaatcaatgg atcaaaccag cagacaatga tcctttgaat
    12361 gacaaaaatg taagcaaatt catttcggat aaagagggcc ttgttacgac aggacaacgt
    12421 ttcttgcctt ctggcaccta ttattttgaa gaattaaaag gtgtggaagg ttacgagatg
    12481 gatgaagcaa gtaagagaat cgaggtcatc atccctgaat tttgggtgga tgcaaaaggc
    12541 caaccacaat atgtagtagt taacggacag gagatggctg aattagaatc cgggaaagtt
    12601 ccagtctctg cctatgaatc cgcaacacca cgtgtttata acgagaccgt aaaagaaaca
    12661 acggacaaac cgaaagagcc gagtggtcca agcaatccag gtcaatcagg aaaatggtcc
    12721 gtattcccgc aaacgaatga agcaagaacg ttgatttctt tactaggaat tcttttattg
    12781 ttaggtgctg ttttattaat aaaaaaagaa cggggagaaa aaaatgaata aaaaaatgat
    12841 tgtcacggga atgagtgtac tgttactcgt tactgcggga atagggattt tcgggaacgg
    12901 aaaactagtc aaagccgaag aagtcgcaca aaaaccaagt gaagtgacga ttacattaca
    12961 caaaaaaggt ttctcatctg ttccggaaga acgaccgaat agcggattgg tttctacgga
    13021 ttttggtgaa gaaaatattc caggagtcga ttttgatttg tttgacgtaa cggaagtata
    13081 ttacgatttg attcgagata atcctttaac accggaacga gaagacggtc ttaattctgc
    13141 cgaagcgatt gaatggattc aaaaacggca cacagaaagt tggtttttaa aatatcgact
    13201 gacaagtatt gacaaacaaa caacgaatga agctggagaa gcggtattct ctaccgtcca
    13261 agtgacggaa gaagcacctt cttctagaga taaagtctat cttttcttag aaacttattc
    13321 gccagctcat atttccagaa tcgcgtctcc aatggttgtt atgatgccag tgatgatgcc
    13381 tgatatggtg gatggcgtgt gggatggttc tacatggaaa gacacataca atacagatgt
    13441 gcatttgtat ccgaaaaacg aaatcagaga agcagataaa cagatgaatg tagaggaaag
    13501 cgaccttaga caagtaacga ttattaatga agctggagaa caagaaacga tttcttatat
    13561 tgatctagaa agaggaaaaa cggcttctta cacgatcaca gcacctattc cttatttcat
    13621 cgattctgtc cttgaaaacg gaagcgctgt tatcaaaaat tacaaaatca cagatacgcc
    13681 tacagttggt ctaacttatt atgatcagga aatagaagtg cgagccggcg agacgatttt
    13741 aacaaaagga caagattata tagtagaagt agtaagcaac gggtttgtcg tgacgatttt
    13801 gacagaagaa aatggagtag ccaaagtaga tactttaggt agattagcag atgcacgtgg
    13861 aggagattta acgatcacgt ataatttgaa ggtttccaca gaactagaag cggatgattt
    13921 ccataacaac acagcggtta ttgaaatcgg acgaaatgat gaatttgatt atgaagaagg
    13981 agtagagccg ccagagaaag tcacaacagg tggaagaaag tttgaaaaat atgatgcatc
    14041 aagcagtgaa ttgcttaaag atgcaagatt cgaattgtgg aatgaagacc gttctgaata
    14101 cgcaatattt tataaagggg agtcgccttt agctgtttat gaatcaggag cagatagaat
    14161 tgagtgggca acaagcggac aagcaacaga atttgtcgca gatggaaatg gatactttga
    14221 ggttcaaggg ttagattatg gaacctatca aatgaaagag accatggcgc cagaaggtta
    14281 tgtacttcca acaggtgagg cagcctttac tgaatttatc atctcttatg gaagctataa
    14341 tgaagaaatt cagatcgtag gagtagaaaa tccagggcca gaaagagtac cgaatatgaa
    14401 acgcgggtca cttcctgcta ctggtgggaa cgggctcttg gcattcttac taatcggaat
    14461 aagtttgatg attggagcgt acagctggta ccgtaaatct aaaatgaagt ctgaagtata
    14521 agcaacaaaa ataagctgca gcaatcggtt ttataaaggt tgctgcagtt tttttaaata
    14581 agaaggtgaa gaaaatgaaa aagaaaatag gtaatagacc tcaaacccaa atcagtcttt
    14641 tattacaatt atttatggca gtgatgtttc ttgccggagc tgcagttttt acatatccat
    14701 ttttagcaga tgctctcagc aattatttgg atcaaagaag gatcgagaat tatcaaaagc
    14761 agcttgaacg cgataaagaa gaaaaacatg agcaacaatt agcagtgcat gaaaagaaaa
    14821 atcagacgct tgctcataca tcagtaatcc caggaatggg acaagtcaaa gatccttttg
    14881 agcaagcggt tagaaatgtc caaaatccag gaaaagaata ttacgaacaa catatgatcg
    14941 gagccatcta tattccgaaa atcaatgtta gcttgccttt atttgatgaa acgaatgatc
    15001 ttttgttaga tagaggagcg acagtcctgc aaggcacctc ttttccaact ggaggagaga
    15061 gtacacattc cgtgattacc gcccatagcg gtattgctga gaaaaaactc tttacggatc
    15121 ttgaagaaat ggagcaagat gaccgattct atcttgaagt gtatggtcgg atgctcgctt
    15181 atgaagtcgt tgagaaaatt gttgtccttc caacaaaaac aaatacatta gcaattagag
    15241 aaaagcagga tctagtcaca cttatcactt gtacacctta caccgtgaac actcaccgat
    15301 tactagtgac aggaagacga gttcccttta cagaagaagt ctcttcaaag atagaacaga
    15361 caaaaaaata ccatctttat cgattactta tactccttct aggtatattg ctagtgctaa
    15421 cgcttttcgg ttattggtgt tatcgaaaat ttaaaagaca aaaacaacga aagaaaaata
    15481 acagataata gaccaattat taagaataac ataataaatt taagctgctt ggaagagaca
    15541 aacgcgatta aatcgcatat ttccagtttt ttttaacgtt tgctaaaatg acattttcca
    15601 ggtaagccaa cttgtttcac aagggcttct ttgatattgt agtagcaaga agaaagatgg
    15661 agggattttt tatggcaaaa atcatggtag tcgaagacga agagatcatt cgacaattga
    15721 tcatggagga acttgagaaa tggcaatttg aaacctttgg aacgaccgat ttcaatcaag
    15781 tgttctcaga ctttgaaaga gaagaaccac agcttgtatt actagatatt aatcttcccg
    15841 ttttggatgg ctattattgg tgtcaaaaaa tacgtgaagt atcgaaagta ccgatcattt
    15901 ttatctccag caggaatacg aatatggata tgatcatggc tatgaacatg ggggcagatg
    15961 attttgtgac aaaacatttc caaatcgatg tactgattgc aaaaatcaat gcattacttc
    16021 gccgttctta caactacaca gaattatcta gtgaaatgat gagccacaat ggcattacgt
    16081 tgaatgttga caacggaagt atggaaatca acggagagat cattgatttg agcaaaaatg
    16141 aatatcgtct attatttatt ttgatgaagc acaatggaaa gatcttaagt cgggaaaaat
    16201 tgctgcgagc actgtgggat gacgaacgat ttgtagatga caatacattg acagtcaaca
    16261 tcaatcggtt acgccgaaaa atcgaacagg caggtatcca aggatatatc gaaacaaaag
    16321 taggacaggg atatatcgtt ccctgaaagg atgagcggtt aagatgaatt tttttaaata
    16381 tttgaaagat cagtggacat tgatcttggg atggctgttt tttattgcat tgacgatctt
    16441 tgtgatgtgg ctggcgccaa acatcaaagt tgatttgagc acaattgctt atttagcttt
    16501 gattgagggc gtctttctga ttttttatct gctgggtggt tacttgagca agagaagatg
    16561 gtggggaaaa ttagccgaca tacaaaaaac ttcccctttg caaaattatt tgagtggtgg
    16621 gaggacagaa gaagaaaaac tggtagaaga atatatcaat cagctgatcc gtgaacacca
    16681 agaagtaatg caatcagcaa tcagtaatca acaagaccaa aaagattata ttgactcatg
    16741 ggtccatgaa atcaaagtgc cactttccgc ttctaaattg ctagttcgtt cgatcgagtt
    16801 cgatattgac gatcaaaagt atacattact agaaaatgaa ttatcaaaaa tcgatgaata
    16861 cgtagaacaa gttttgtatt atgcaagact cgacagtttc tcgcgggact acctcatcca
    16921 agaatatgga ctaaaaggaa tcattcagcc tgtgatacgc agtcaggcaa attattttat
    16981 ccaaaaaaat attcgctacg agatcgttgg tgaggatcat actgttttga ccgatgctaa
    17041 atggatcgct tttatcttca accaattgct tagtaatgcc atcaaatata cgccggatca
    17101 cggaaacatc attgtgagca tagaaaagga agcgcaagga gtcagccttt cagttaaaga
    17161 ttcagggatc ggtatcccag cggaagactt gaaacggatc tttgataagg gatttactgg
    17221 gagaaatggt cgacttagca aaacacattc aacaggatta ggactttatt tagcaaaaaa
    17281 tctggccgaa aaattaggta tccatttgac ggctgaatca actgaaggaa aagggacaac
    17341 aatgacttta tttttcccaa tcttaaattt ttataatgaa aaacgttaga aaatcgttag
    17401 aaacgaaaaa aagtgcttgt gttgttaacg ttttcgctga taagtttctt atcatgtgaa
    17461 ataaaaggag attggttatg gaaattttaa tagcattagt ccctatgttg gcatggggta
    17521 gtattggttt agtaagtggt aaaattggtg gcgatgccaa tcaacaaaca ctaggaatga
    17581 cgatcggtgc atttctgttt tctttagttg tctttttcgt tgttagtcct gtaatcaccc
    17641 cttggatttt cttgatcgga tttttatccg gcttagcttg gagtgttgga caaaatggac
    17701 agttccatgg aatgaagtac atgggtgttt ctgtagggct accattgtct actggttttc
    17761 aattaatttt gaatacgatt gcgggagccg ttttcttcca tgaatggaca caaacgaaag
    17821 attatgtata cggtattatt gctttagctc ttcttgtaag cggtgcgtat ttgactgccc
    17881 gacaagatga cgaaggaaaa atagatacag ataataaaat gcttgatttt ggtaagggtt
    17941 ttagagcatt gatcttctct acgattggtt atggtgtata taccatcatc gtcaactggg
    18001 cgaatttgga tgcgatgtca attatcttac ctcaaagtat cggaatgatc ttaggcgcaa
    18061 gcttctttgc tttccgtaaa gttaaagtcg atcagtttgt gtggaaaaac atgatctgtg
    18121 gattattatg gggcttagga aacatctgca tgcttctaac agtcaaatca cttggtttag
    18181 ctgttggctt ctcactttca caaatgggga tcattatctc tactttaggt gggattttca
    18241 tccttggcga aagaaaaacg aaaaaagagc tcatttatgt tattgttggt tgccttttag
    18301 tcattttagg tggtatcctt ttagggtata tgaaaacagt ttaatcgctg ttttccaacc
    18361 taaaatgggg aggaacttca atggatttgt ttcgtaagaa agagatcaat ctcaatcata
    18421 cgagtgagat gaaaaaagaa ctaaaaacgt ctgatttgat catgcttgga attggtgcga
    18481 tcatcggtac gggaatattt gtggtgacag gggttgcggc caatcaaaat gctggtccag
    18541 cactgtcact ttcatttgtc ttagctgcaa tcgttgtcat tttgtctgga ctaagtttcg
    18601 cagaatttgc ttcgcgagtg ccagtgattg gtgggccata tgcctatctg tatgttgtat
    18661 ttggtgaatt tgctgcctgg ctgacagggt ggttgttgat cggggaattt ttactagcgg
    18721 tttcctctgt tgcttccgga tggtctggct atatgcaagg ttttttgaaa agtttagggg
    18781 ccgaacttcc acaagcatta actggcgggt acaatccgga aaatggtaca tacattgact
    18841 tgattgcggt gctcgtagtg atattcgtca cttacatcgt gtcgcttgaa gccaagaaag
    18901 cattacgttt gaataatgcc atggtatatg tcaaatttgg tatcatcgcg ttgtttatca
    18961 ttgtaggaat atttttcgtt aaacctgata actggcagcc attcatgcct tttggttttt
    19021 ccggtgtgct agatggtgca gctcttgtgt tttttgcttt cttgggattt gatgccgttg
    19081 caatggcagc cgaggaagtc aaaaacccgc aaaaagatgt gccaagagga atcattggtt
    19141 cgatactgat tgctactgtc ctttacatca tcgtgacgct gatcttgaca ggaatcgttc
    19201 cgtatactga actaggagtc aatgacccag tggcttttgc aatgaggtat gtaggacatg
    19261 gaacagtggg cgcggtgatc gctgtagggg caattttaac gctactgacg gtaacgatct
    19321 cgatgatgta ttcacttgct cgtttactgt ttgctgtcag caaggatggc ttacttccta
    19381 agtttatgac agaaatcgat caaaagcatc gtacaccgaa aaaagcgact tatgccgctg
    19441 gtgtggcagc tgtattcttt gccggatttt tcccattgaa tgtattagcg gaactaacga
    19501 atatcatggc gctcgcctat ttgatgcttg ttagtctagg cttactgaag ttgagaaaaa
    19561 tgtttggcaa accaaaagct ggagaattca aagtgccatt cgttccgcta ttgccaatcg
    19621 tctccatctt gacttgtatt gtattgatgc ttcgtctgca aactgtgaca tggatcgtgt
    19681 tcggtatcgt aatggttatt ggactgatca tttattttgg ctatggctat ggacacagca
    19741 agatgaatga gaaatagact ccaatggaga agttagaaga aagataagaa aaacgcccat
    19801 caacagttgt aataaactgt tgttgggcgt ttgttttagt aacaagcgaa aggtgatcta
    19861 ttgggttaaa tccaacagtt tttgtttcag atcttcttcc atgtgtccaa gctctacttc
    19921 tgcatgacga cgtttttctt ttccttcttt ttggatacgc aatgtctctt ggattgtttc
    19981 gatcaaatca ttttgagtgg tttgaagggt ctcgatatcc acgattccac gttcattttc
    20041 tttcgctgtt tcgatggctg agattttcag catttctgaa tttttcttca ataagtcgtt
    20101 tgttgtttca gagacttggc gttgagcagt tactgcatct ttttgacgaa gcaaggtcaa
    20161 tgcgatcacg acttggtttt tccacagcgg aatggctgta gcgatagatg cttggatctt
    20221 ttcagccaag gcctgatttg tgttttggat caaacggatt tgtggtgctt gctggatcgt
    20281 gatctgtcta gctaagcgaa gatcatgcgt acgtttatcc agacgatcta ggaattgcgt
    20341 gtaatcattg gcaatctgaa catccatctg atcgccagtt tcttctgctc ttttcatcgc
    20401 ttctggaatg atcgttgttt gaagttcttc cattttcaat tctccagcag cgatataaat
    20461 attcagtgca tcaaagtagt ctttgttttt ctggtaaagc tgttctaaca tcagattatc
    20521 ttttagcaga ccgtcttttt ctttatctaa tttcactgaa atcttgtcaa tttgtgcacc
    20581 aatcttttga tattttgctg tcacttcata gatcgattgt ttgaccttcc cgaagacacg
    20641 ctggaagatg ttcccttctc cggcacgcag ttcgtcagga ttggcttctt ggagacgata
    20701 catcagttca gttaaggaat ctcctacagg accaatatct tgtgcttgca catgattcaa
    20761 catcgattgt gagaattcac ttaattttgc ttgggcagcc gatccgtagc tgatgacaga
    20821 ctgtgcatca ttagcatcga tttttgctgc tagctgttta gcttgttctt ggcgttccgc
    20881 cggtagtttg tcaattaaac gagcagctgt ctgttgctct tggagctgat caatctccgc
    20941 ttgttgtgaa gtggtcagcg tatctgttgg agttgaaaac gggttgttca ataaatcttc
    21001 aagtgtatcg ttaactggtg taacatcttt tgtttcagtc ggtttatgat ccataaaaaa
    21061 ttcctccttg ttgaatctgt tctcgattct tacatgaatg tctctacttt agaaaaagta
    21121 ctcgaaacag ccagacatca tctgaatgtt ccgagtcttc ttttatgaac aaaagagtta
    21181 atccttttgt tctggttcat atttattatc acgctttaag ctctgtttcg caacagatat
    21241 ctctacgtcg atatcatcta gatcctcagc aacgaatttt tgataatctt gtgcgattaa
    21301 agctgacagc tggtcgatga tctgcgcgct ttcttctagt ttttcatagg tctgtttgtt
    21361 tttgatctca tggtcattga tctcattata cttatttgtc aaatcaacta gattcggcag
    21421 atgcgtataa agaaactgac ttgcttcatg cagtcggttt ggttctttca ccaattcttt
    21481 gaataaagct ttcgctgctt tgactggatc atggcggaga tcgattgctt taagtttggc
    21541 tgtctgctgc atattcattt gcaattgatt gatctcattt tttgtttgat tcattgtttg
    21601 tcgaaaaaat gaaatttcat tcgctgtcat accgcttttt tcatagtgag tttcaagttc
    21661 gtcagtcagt gcaggaattt cttcttgttt tccctttttc ctgcgcgaac caaagagtcc
    21721 ccacaaaact aaaactacac ctatcaataa aagtcctaga acaaaagaaa aactattgtt
    21781 tcggacaaag agaaatagca agaccaatgc tagaagccac ggccaatttc gttttatcat
    21841 aataaaccct ccatgtcatt tatcgttgag tattgaattc ttttcgttat tcgattacat
    21901 acttatttta gcatacggac tactagaaag agtatcggac taaagaagga ttttctatat
    21961 ttagagaaaa tctaaggttt atctatggta aactaataag gagtttttga gaaaaattga
    22021 aaaatgaaaa aaaagcagtt atgattacaa agaaagcagg agaagatatg ctgaacaatg
    22081 agcaatttga agaaaaaaca atcagtcgca aagaaatcta tagcggaaaa attattgatg
    22141 ttgctgtaga cgaagttcgt ttgccagatg gaggaacttc gaagagagaa ctggtatttc
    22201 atcctggcgg tgtcgggatc attgcttttg acgaacaaga tcgtctgctt cttgtcaaac
    22261 aatttcgaaa acctttagaa aaagtgattt tggagattcc agctggaaaa atcgatccag
    22321 gagaaggaca aaatcccgaa atgacagcag ctagagaatt agaagaagaa actggctatc
    22381 gtgcgaagtc gctgagccat cttacttcga tgtatctttc accaggtttt gcaaatgaag
    22441 tccttcatat ttatcatgca caaggggtag agaaggtcga gaatccttta gcacaagatg
    22501 aagacgaagt actggagttg tatcatctaa ctcttgaaga ggcgcaacaa gcgatgaaag
    22561 atcagttgat ttgtgatgca aagacgattt atgcgattca atactgggaa ctattgacca
    22621 aaggaaagta gagggatcgg cttatggcca aaggaccgct tattacgcga agcgaacttc
    22681 gcaagcgaca acaagcacaa gcaagcgagt cattaaaaaa acagcgaaaa gcagaaacag
    22741 cttatcagca agaagaaaaa aagattgcca gcttttatcg taaagaaagc aaaaagaata
    22801 aaccaatcac gaaaacaagg atcagcgaac gggaaaagac gaccaaatgg aactcttttt
    22861 tgatgaaatc tcttattatt gttatcctaa tgttatgtgt ggttttttta gcaatcgcat
    22921 ttatatagaa agaaggaatt aaaaagatga aaatcggtat tatcggcgca atggaagaag
    22981 aagtaaaaat tttgagagag aacctaagcg aaccattatc atgggaacgc gcaggcgctt
    23041 tatttatttc aggttcatta ggtaaccacg aagtgatcgt cgtacgttcc ggtataggaa
    23101 aagtcttagc atctattacc acaagtctgt tgatccaaca atacggcgtg aacatggtga
    23161 tcaatacagg ttcagctggc ggaatcggtg aaggcttaca agtaggtgac atcgttatat
    23221 cagacaaagt agcttatttt gatgcagatg caacaggatt tggctacaac ccaggacaat
    23281 taccaggaat gcctctatat tatgaagcaa gcacatattt gcgcagtgaa atggttaaag
    23341 cagcaaaagc aacgaatttg aacgcaaaag aaggattgat cgttacaggt gatacatttg
    23401 tcgactcgcc agataaaatc aaagaaatct taacgaattt ccctgaagca ttagcttgcg
    23461 aaatggaagg agccgctgtt ggacaaacag ctcgtcagtt caatatccca tttttgatcg
    23521 tacgtgcaat gagcgacaca gcagatcact cggcgacaca aagctttgac gaatttatcg
    23581 aagacgcagg caaacgatca gcagaaatgg tgatcgaatt tgtcaaacat ctcgtataag
    23641 gaggactaag atgaacgcat tgatctcgat tgactacaca tatgattttg ttgcagagga
    23701 tggaaagctg actactggga cagcaggaca agtaattgaa aaaaagctga ctgctcatac
    23761 aaagaagttt attgatcaaa aggattttgt cgtttttgcc attgatgcgc atgatccaaa
    23821 agattccttt catccggaga accgcttatt tcctccgcat aatgtgatag gaacgggtgg
    23881 acgggaattg tacggctctt taaaaagttt ataccaagac tatgagcaag aggaaaatgt
    23941 ttattggata gacaaacggc attattccgc ttttagtgga acagatctag acatccgttt
    24001 gcgagaacgc cagatcacag atatttattt gaccggcgta tgtacagata tctgtgtgtt
    24061 gcatacagca gtcgatgcgt ataatctagg atacaaactc catattttca aagattccgt
    24121 ggcaagtttc gatcccgttg gacatgaatg ggcattgaga cattttgagt ccacactagg
    24181 cgcagaaatt ttataaaaaa acaaagtgat ttgataaaat aaaaagcaca aaataataaa
    24241 agaagagcat aaaacaaacg actttgtttt atgctctttt ttatacgtta ttttaaaaaa
    24301 acttattctt tttccgttaa taaatctctt tattatcaac aacttaagtt atattttttc
    24361 tttttataaa atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt
    24421 aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg
    24481 tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc
    24541 tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa
    24601 cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga
    24661 agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga
    24721 agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta
    24781 tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt
    24841 ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa
    24901 ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg
    24961 atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta
    25021 ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca
    25081 agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc
    25141 aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt
    25201 ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa
    25261 tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga
    25321 cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg
    25381 agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc
    25441 ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga
    25501 agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat
    25561 tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc
    25621 ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct
    25681 taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact
    25741 gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga
    25801 agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg
    25861 taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag
    25921 cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat
    25981 cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa
    26041 cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc
    26101 tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt
    26161 acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa
    26221 tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag
    26281 cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac
    26341 atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac
    26401 aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg
    26461 ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt
    26521 tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa
    26581 aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa
    26641 tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag
    26701 ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta
    26761 ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg
    26821 cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg
    26881 agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct
    26941 aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca
    27001 tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga
    27061 tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata
    27121 tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc
    27181 gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact
    27241 gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga
    27301 agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt
    27361 ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac
    27421 ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca
    27481 gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga
    27541 aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc
    27601 ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga
    27661 atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta
    27721 tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga
    27781 ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt
    27841 tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga
    27901 cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
    27961 caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag
    28021 gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag
    28081 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tacttgaaac
    28141 taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat
    28201 aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga
    28261 agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat
    28321 acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt
    28381 gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt
    28441 attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag
    28501 aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa
    28561 tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga
    28621 attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc
    28681 agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa
    28741 tctcatgagc gataacagct ccgattccac cataattagc agagcttgat tgttctagac
    28801 tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat
    28861 aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc
    28921 atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag
    28981 taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg
    29041 ggtagcctac ttggatacct aagttattta attttatgac ggctttttct tttgttgcat
    29101 ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca
    29161 tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa
    29221 ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt
    29281 cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata
    29341 gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat
    29401 gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt
    29461 tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt
    29521 atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt
    29581 ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg
    29641 cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga
    29701 aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa
    29761 ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat
    29821 ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat
    29881 tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag
    29941 catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt
    30001 tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca
    30061 aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag
    30121 ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc
    30181 gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa
    30241 tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt
    30301 ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt
    30361 ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca gcactcagca
    30421 gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag atcaaagtta
    30481 tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat atacttatca
    30541 agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg agttatctag
    30601 atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt caggaaagga
    30661 actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata agaaatgggc
    30721 taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg cctattatca
    30781 agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata cggctgattc
    30841 gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc ctacgattcc
    30901 ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc atgagcgtgc
    30961 acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg aaggcaaacc
    31021 tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt gggtgacagc
    31081 gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg acaactgggg
    31141 cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac ttccttaaga
    31201 atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa gaatagagaa
    31261 gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag atttttagtt
    31321 tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca gaattaggta
    31381 aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa gaaagtattc
    31441 gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat tactatatgg
    31501 aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc aatgtcgtat
    31561 tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa ccgatcactg
    31621 cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg actttagatc
    31681 tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta tttacgatgc
    31741 cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc gtagttgttt
    31801 cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg aacagcacat
    31861 tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg atcggtgatg
    31921 ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca acgcttgcac
    31981 gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt gcgtcacatg
    32041 gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa gatatttgta
    32101 tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca atcgttacat
    32161 gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg atgagtccgt
    32221 tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa gaagttgtga
    32281 cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc ttttttctat
    32341 gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg ctattttatg
    32401 ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta tttatctgga
    32461 tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg caatcatgca
    32521 agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc atgaaaaatt
    32581 ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg agatcatctt
    32641 taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt tctcgcgtca
    32701 aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc ttaataccat
    32761 gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg aaaaagggaa
    32821 tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg tctctgtgat
    32881 gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg aacttttagc
    32941 ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc aaacgatcca
    33001 tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca atgggccaaa
    33061 aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc ttttaggcgg
    33121 ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct ggggtatggc
    33181 aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca aatataaagg
    33241 attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc aagtaaatgg
    33301 tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg aagcaaatga
    33361 tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt cagcttgtac
    33421 agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag atacaccgat
    33481 actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg acatccagta
    33541 tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat tcttatatga
    33601 acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa cctagtaaaa
    33661 atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa atcatggcat
    33721 ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat cctgatgcaa
    33781 aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat tatcaattga
    33841 tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa atcactgtat
    33901 caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg aaagcagtca
    33961 atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt ctgatggatg
    34021 ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata aaaagacaac
    34081 tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc aaattagttg
    34141 tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct gatttattgt
    34201 ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg tattttttga
    34261 aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa aagaaaagag
    34321 ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga aattatttgt
    34381 tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa tatgcgatca
    34441 tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct actttactag
    34501 gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat cgctgggata
    34561 aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt attttagcag
    34621 tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct ttgtttatgc
    34681 gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg acaatcgttc
    34741 catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca gcgaatttca
    34801 tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat taccttattt
    34861 tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg aagatcccaa
    34921 aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca aaatttgggc
    34981 tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt gcatttgtga
    35041 tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat tactttggtg
    35101 gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg ctcttaggcg
    35161 gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt attatagcct
    35221 acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat agtcaagggt
    35281 tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat ttcaatacac
    35341 tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt gtcttaggga
    35401 acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca ggaccattag
    35461 ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt ttattatgcg
    35521 gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta cagaaaaaac
    35581 tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg tgacagaagt
    35641 cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta ctcaaatgaa
    35701 aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt tttcacgacc
    35761 tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat aacggagtgc
    35821 caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct acattgacat
    35881 cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg atagtgtcgt
    35941 taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg aatttaacga
    36001 tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta ggatcatttt
    36061 tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag tggattgttc
    36121 cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat tgcggatgca
    36181 tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca ttccagctag
    36241 tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca aagtcgtttt
    36301 ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc atgtatcttt
    36361 tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca taacatctaa
    36421 aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat tttgaaaaat
    36481 agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct ttttatgagt
    36541 atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg actatcatgt
    36601 ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt aagggatgaa
    36661 attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt caactcgtag
    36721 atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc aaaacacgcg
    36781 ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg ttaaaggaac
    36841 aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac gaaaacggtg
    36901 tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa atcgggattc
    36961 cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa tatttcctag
    37021 ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa gaaatcaaat
    37081 tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct actggacatt
    37141 atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga gtagacaata
    37201 ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca aaaacgatgt
    37261 tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc gcaggccttg
    37321 cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag aacttcaaac
    37381 aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt gatggtgaag
    37441 tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa ggactaggga
    37501 tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat ctagcgacaa
    37561 acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca agcttggatg
    37621 caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag tgtacagcga
    37681 aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat gataatcgtg
    37741 cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca gttgtatttt
    37801 atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa gaaacaaaag
    37861 ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta acaaaagaaa
    37921 caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt ttattcattt
    37981 tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat aaagttgctt
    38041 tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga tttgttcaat
    38101 tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca aatggggact
    38161 ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca caagtgatct
    38221 ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg ggattggtat
    38281 tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc tagatggtgg
    38341 tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca gtggttgggg
    38401 ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga tgagcgccat
    38461 gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt tatttgcaag
    38521 tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag cgagcgtcat
    38581 caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa tatttgtatt
    38641 tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat ctgataatct
    38701 agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt gtttgatggt
    38761 tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc gcgcgaagaa
    38821 acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt ttatctatat
    38881 ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa ctatttcaca
    38941 acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag cgctgatcaa
    39001 tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc tcccagcaga
    39061 aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa aaacgaataa
    39121 aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc tattcttatt
    39181 gactttctta gttactgatt atgcctatca atttgcctat tctttatgta cagcggcaat
    39241 tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa aagaaggaat
    39301 caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg gcatcatttt
    39361 agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt tttgcttcta
    39421 ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag taatgattgg
    39481 gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct ggatcaagat
    39541 ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg ctaaataaat
    39601 gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg acattagacg
    39661 aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc aaaaacgaac
    39721 cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc gcttctcttt
    39781 atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt gctaatcttt
    39841 tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt cctacttatc
    39901 agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt catttgaagg
    39961 aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa cctgaaacaa
    40021 aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat cggaaaactt
    40081 tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata gatgaagtat
    40141 atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat gaaaaaggta
    40201 tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt ggctggacag
    40261 ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg atgatttgcg
    40321 gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa aaaatattgg
    40381 cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg gtagccaatg
    40441 aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt aaattagcca
    40501 tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga gtactattag
    40561 taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt tgtaaaaaag
    40621 aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag gcctaaacaa
    40681 aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt tacgcttatt
    40741 gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt tcttctgcag
    40801 tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg accaaaacaa
    40861 gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca ctggagccga
    40921 tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg aagatccgtc
    40981 cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg agcagacaag
    41041 catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc gtgacgaatg
    41101 ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt ctttcactcc
    41161 caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca atcccaggta
    41221 tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc aattggcgaa
    41281 tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc atcgttgtga
    41341 cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt ttggcagaaa
    41401 ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga ctcaacaaga
    41461 agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca aacgctgaag
    41521 cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca gttgctaagg
    41581 ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg gcagtggcat
    41641 tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat tgatacacac
    41701 tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca cgcaacagca
    41761 tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata agcgtagtca
    41821 ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac gtactataat
    41881 tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat ccgataccag
    41941 ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct cttttcctaa
    42001 ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa ttttcatcta
    42061 cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt ttttctcttt
    42121 ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa catggaaatg
    42181 aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact agcagaagag
    42241 atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat caaggctccc
    42301 tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac agcgaaactt
    42361 ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg tcaggaagca
    42421 ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc agtgtatgag
    42481 ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat ccatgaaacg
    42541 aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga aaaagttcat
    42601 cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt tttaggatac
    42661 tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat caataacttt
    42721 atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat gttcattaaa
    42781 tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa gatgagtcta
    42841 gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc ggggacaatg
    42901 agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa acattcgagt
    42961 ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat caaaattatg
    43021 aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga gatgagcgct
    43081 ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg ttgaaaaatc
    43141 atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat tctagcggaa
    43201 aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa gcacaaaaat
    43261 tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt aggattgaaa
    43321 atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt gccaatgatc
    43381 cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa agcaatcaag
    43441 cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc cggaagagaa
    43501 ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc agtgaagcgg
    43561 ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca actaagacct
    43621 gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc ggactggctg
    43681 gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt atcagcaggc
    43741 cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat cgagcagttc
    43801 cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc caaaatgctt
    43861 caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt tcaattgatc
    43921 agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga atctgcgcca
    43981 ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg ctattctgca
    44041 aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt tacttatccg
    44101 cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga acgattgcaa
    44161 gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc catcgtccaa
    44221 cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt gccaactgaa
    44281 aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca aatgattgtc
    44341 tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa agttacggtt
    44401 gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc aggatggaaa
    44461 ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag aatcaatccc
    44521 gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga agcaagcttg
    44581 cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga gattatcgag
    44641 aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt atctcctaaa
    44701 ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt gacagaaagc
    44761 tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac cttcttgaat
    44821 ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa acaagaatat
    44881 caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct gcgcgagaaa
    44941 aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta ccgcagaaga
    45001 aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat ggaaatcatc
    45061 gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga aaaattgctt
    45121 gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt gctgaaaaaa
    45181 tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa agcagcaatg
    45241 aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagcttcaa gaaataagaa
    45301 ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc
    45361 tacttctgga acaccattta ttcggaaaaa aggactgtga caagactttt gtcacagtcc
    45421 tttttcattt aaaaaaatgt ttacttcatt tgacgtatat tattggtgag gcttcaattc
    45481 atttggcttc actttttctg tttctcttat gatataaaca ggacgctgtt tcgtttctaa
    45541 gaagattttc ccaatatatt tcccgatgat tccaagacat aacaattgga ttccaccaat
    45601 gaacaggaag atcgagacca tggaaggcca tcctgatgta ggatcaccaa acaacaatgc
    45661 ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca caagacaaag cgccgataaa
    45721 tgaagcaatc gttaacggtg catccgagaa gttcacgata ccatcaatcg agtagttgaa
    45781 taatttccag aaggaccaag atgtttcgcc agccacacgt tcgcggtttt cataagaaat
    45841 atattctgtt ttaaatccga cccagctaaa taatcctttt gaaaatcggt tgtattctgt
    45901 taattctagg acggcatcca ccatttgacg agtcatcaag cggaaatcac gagcgccatc
    45961 gaccatttct gtatcgctga ttttattgat caactggtag aattttttcg caaagaaact
    46021 gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg ccgacacaat cgatatcaga
    46081 agtagtgatc cggttgatca tctcaggaag cagttcgggc ggatcttgca gatcaacatc
    46141 catcacggtc actaaatctc ctgtagaagc ttgcaatcca gcgtacagag ccgcttcttt
    46201 cccaaaattt ctggaaaatg agacaaagcg gacataagga taagctgagg ctagcttctt
    46261 gattgctgct aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt cgaactcatg
    46321 ggcacctttg aatttttcaa cctcatcaaa aaaaagagga acagtttttt cttcattaaa
    46381 acatgggaca acaattgata ctttcatcgt ttctatcctt tcaatcgtat gtttatatat
    46441 ggtttacatt caaaatttaa taaaccttaa ataacacctt taaaatttga ttttacgtaa
    46501 aagaacaggt attctttttg taaactaacg tatttagttt accatgtttc ctcggtttat
    46561 caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag aaaaaggagg
    46621 aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct atcggctttt
    46681 tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca atttcagaaa
    46741 gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa gaatattttg
    46801 cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg ccttttcagc
    46861 aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg tataaagaag
    46921 tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca aatcgaacca
    46981 atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga aaactgacag
    47041 gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg ttgttagaag
    47101 gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga gaatcacaac
    47161 tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag ccatcactgt
    47221 ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt tcatgaattt
    47281 tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc agctgctgat
    47341 aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga ccaaacaaaa
    47401 gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct gcgtatcagt
    47461 tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca gatagacaac
    47521 aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga caacagaatt
    47581 gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac cacccaaaag
    47641 tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag ggagagtcaa
    47701 catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt cttcctcttt
    47761 cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag cgtttcctcc
    47821 agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca agtaagcacc
    47881 gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac caatttgagc
    47941 aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta atagtttgct
    48001 ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat ttcctaataa
    48061 aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa tagcaagaag
    48121 cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt cgcggaccgc
    48181 tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg ttcctttcgt
    48241 caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt tgacaaaaga
    48301 acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaataaacg gtgttccaga
    48361 agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
    48421 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact
    48481 tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa
    48541 gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt
    48601 gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc
    48661 aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca
    48721 tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt
    48781 tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca
    48841 gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat
    48901 atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt
    48961 tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt
    49021 ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc
    49081 tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa
    49141 agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa
    49201 gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg
    49261 atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc
    49321 ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg
    49381 ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc
    49441 agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa
    49501 agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag
    49561 gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat
    49621 catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg
    49681 ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca
    49741 tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta
    49801 atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc
    49861 caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta
    49921 agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt
    49981 ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac
    50041 ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt
    50101 tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc
    50161 tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt
    50221 ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac
    50281 gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag
    50341 ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt
    50401 tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag
    50461 attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg
    50521 ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat
    50581 tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg
    50641 ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt
    50701 ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt
    50761 tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa
    50821 attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt
    50881 aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt
    50941 caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat
    51001 ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga
    51061 acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt
    51121 taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag
    51181 taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata
    51241 taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga
    51301 atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc
    51361 aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg
    51421 aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga
    51481 attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca
    51541 attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg
    51601 aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc
    51661 gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct
    51721 tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg
    51781 gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt
    51841 ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta
    51901 gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta
    51961 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
    52021 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
    52081 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
    52141 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
    52201 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
    52261 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
    52321 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
    52381 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
    52441 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
    52501 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
    52561 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
    52621 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
    52681 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
    52741 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
    52801 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
    52861 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
    52921 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
    52981 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
    53041 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
    53101 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
    53161 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
    53221 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
    53281 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
    53341 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
    53401 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
    53461 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
    53521 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
    53581 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
    53641 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
    53701 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
    53761 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
    53821 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
    53881 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
    53941 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
    54001 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
    54061 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
    54121 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
    54181 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
    54241 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
    54301 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
    54361 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
    54421 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
    54481 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
    54541 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
    54601 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
    54661 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
    54721 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
    54781 caaacatcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
    54841 tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta
    54901 tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt
    54961 aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg
    55021 cgattgggat tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa
    55081 gactgatgac gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca
    55141 tatttttttg acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata
    55201 acagggattg tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat
    55261 gatcaaacaa ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa
    55321 tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac
    55381 tattttagtt ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt
    55441 aggttaccga ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa
    55501 tcatcatcat ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag
    55561 tttctttgtt tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat
    55621 caatagcctg gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta
    55681 ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc
    55741 gatgttgaat aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt
    55801 cttagggtat ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc
    55861 ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa
    55921 atttaagaga aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta
    55981 ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg
    56041 tcacgatgct tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag
    56101 gatttttgac attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct
    56161 atctatttaa gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt
    56221 gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt
    56281 ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt
    56341 ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa
    56401 cggtgtggtt tttggaataa aaaaataaca tatctcattt tacacttttg ttattttata
    56461 cttaaaaaat aacgacacag aaaataccgt gtctcagatt tctctactac ttctaaaaac
    56521 tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat atactggcag
    56581 aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa agtaattact
    56641 tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa ttaagacgga
    56701 attcctttct agattgagat attgactagg aagaatgtta agattttaag cctttaagct
    56761 aaaaaaaaac cctttttgtt aaataagggg aatatagcct gtcagtttca aactgcaagt
    56821 cgtcctgctg caataatata gtagattaga gatgaaaata gattttttta aaccaatgac
    56881 tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc tattacggtc tttttttacg
    56941 aacgatttta ttgaagacgg acagttcggg tcaaaaataa aaaagctacg atcagaaata
    57001 agaataaaat gtatcccaaa ataatggcta gacggtctaa cttatttgtc gatagatgtt
    57061 tgtttagaag ttgataggcc cataaaccta tcagtcctcc tgttgtatta gttatcaaat
    57121 cagtgatgtc cgctacgcca ataccgaata ggaattgaat caattctgcc aagaagctta
    57181 caaggaaaat aatccctaat tttttagaaa aagaaagtct cttgaaattg acattcaaaa
    57241 gcaccccaaa aggaaaaaag acaatcaagt tataagtggt ctcacgtaat accgtcgtat
    57301 tttcaaaaga aaaagtagaa aaagggacga gattgacatt gctgtaagaa taagtgagaa
    57361 attgtggaag attaaatgat agcttgaata aaatcaacca taatagaaag aataaataaa
    57421 tccctaataa aaattttgat acataccgca tagacatctc ctccatttaa aaagtatacc
    57481 atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt aaggaccaac gtagttatcg
    57541 tacgaaaatt agaagctttt ttttgttggg aatccggttt ctttaacaaa gctaagatag
    57601 cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt
    57661 ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa
    57721 tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg gttcagcttt
    57781 ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt ttcaataaaa
    57841 tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt gtattatggt
    57901 taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg atctttactt
    57961 ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt ctgatcggtt
    58021 gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta tggcttgaac
    58081 catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta ttccatattt
    58141 ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc atgcagctgc
    58201 caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt tttgccctga
    58261 ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt gaaaaagcaa
    58321 ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt ttctacagcg
    58381 ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat ccttattggt
    58441 tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg aagcatcgac
    58501 aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc ttgtcatttg
    58561 tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga atcgcacaat
    58621 ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg gcgttgatgc
    58681 tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt tttttaggga
    58741 tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt ggtttgcaac
    58801 attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg tgccaaaaga
    58861 ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc cagattctca
    58921 gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat tttatattta
    58981 ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag atggtcaatg
    59041 ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt ttatagttag
    59101 tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat ttaaacaatg
    59161 aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga aatccaaatt
    59221 ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt acaagcaaaa
    59281 aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc taataaagca
    59341 aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc agcattagct
    59401 atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt gaagattcca
    59461 agaactatct atttggcaac aatgaatgct tttactagat ttattcatga acatttccaa
    59521 gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac tagtggcttg
    59581 atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg gtacacggat
    59641 ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc agatttatct
    59701 cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact ttattttcct
    59761 gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa tcttactaaa
    59821 tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt attatcagtt
    59881 gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc ccaaatacat
    59941 gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct tgcaggcgag
    60001 cacattggct ctggcttagg tattcttact tcataagttt agtacttcga aatagtttaa
    60061 aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    60121 cggaa
//
LOCUS       DADQRX010000006        57373 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.006, whole genome shotgun sequence.
ACCESSION   DADQRX010000006 DADQRX010000000
VERSION     DADQRX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 57373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.006"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..2121)
                     /locus_tag="J8V14_000354"
     CDS             complement(229..2121)
                     /locus_tag="J8V14_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920489.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="J8V14_000355"
     CDS             complement(2118..3845)
                     /locus_tag="J8V14_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920490.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="J8V14_000356"
     CDS             complement(3861..4511)
                     /locus_tag="J8V14_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0920491.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="J8V14_000357"
     CDS             4715..5011
                     /locus_tag="J8V14_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBA0920492.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5183..5803
                     /locus_tag="J8V14_000358"
     CDS             5183..5803
                     /locus_tag="J8V14_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBA0920493.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="J8V14_000359"
     CDS             complement(5895..7061)
                     /locus_tag="J8V14_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA0920494.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="J8V14_000360"
     CDS             complement(7073..7426)
                     /locus_tag="J8V14_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBA0920495.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="J8V14_000361"
     CDS             7554..8576
                     /locus_tag="J8V14_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBA0920496.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9015..9449)
                     /locus_tag="J8V14_000362"
     CDS             complement(9015..9449)
                     /locus_tag="J8V14_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920497.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9559..11418)
                     /locus_tag="J8V14_000363"
     CDS             complement(9559..11418)
                     /locus_tag="J8V14_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBA0920498.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11536..12696)
                     /locus_tag="J8V14_000364"
     CDS             complement(11536..12696)
                     /locus_tag="J8V14_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBA0920499.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13003..14427)
                     /gene="araA"
                     /locus_tag="J8V14_000365"
     CDS             complement(13003..14427)
                     /gene="araA"
                     /locus_tag="J8V14_000365"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBA0920500.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14443..15141)
                     /locus_tag="J8V14_000366"
     CDS             complement(14443..15141)
                     /locus_tag="J8V14_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA0920501.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15098..16759)
                     /locus_tag="J8V14_000367"
     CDS             complement(15098..16759)
                     /locus_tag="J8V14_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBA0920502.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16977..18377
                     /locus_tag="J8V14_000368"
     CDS             16977..18377
                     /locus_tag="J8V14_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBA0920503.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18629..19708)
                     /locus_tag="J8V14_000369"
     CDS             complement(18629..19708)
                     /locus_tag="J8V14_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0920504.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19794..20753)
                     /locus_tag="J8V14_000370"
     CDS             complement(19794..20753)
                     /locus_tag="J8V14_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBA0920505.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20765..22288)
                     /locus_tag="J8V14_000371"
     CDS             complement(20765..22288)
                     /locus_tag="J8V14_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBA0920506.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22298..22861)
                     /locus_tag="J8V14_000372"
     CDS             complement(22298..22861)
                     /locus_tag="J8V14_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBA0920507.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22867..23730)
                     /locus_tag="J8V14_000373"
     CDS             complement(22867..23730)
                     /locus_tag="J8V14_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0920508.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23744..24640)
                     /locus_tag="J8V14_000374"
     CDS             complement(23744..24640)
                     /locus_tag="J8V14_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0920509.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24815..26086)
                     /locus_tag="J8V14_000375"
     CDS             complement(24815..26086)
                     /locus_tag="J8V14_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0920510.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26076..27593)
                     /locus_tag="J8V14_000376"
     CDS             complement(26076..27593)
                     /locus_tag="J8V14_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBA0920511.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27791..28681
                     /locus_tag="J8V14_000377"
     CDS             27791..28681
                     /locus_tag="J8V14_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBA0920512.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="J8V14_000378"
     CDS             complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="J8V14_000378"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBA0920513.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29590..31062)
                     /locus_tag="J8V14_000379"
     CDS             complement(29590..31062)
                     /locus_tag="J8V14_000379"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBA0920514.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31699..32061)
                     /locus_tag="J8V14_000380"
     CDS             complement(31699..32061)
                     /locus_tag="J8V14_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBA0920515.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32284..33036
                     /locus_tag="J8V14_000381"
     CDS             32284..33036
                     /locus_tag="J8V14_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA0920516.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33176..34099)
                     /locus_tag="J8V14_000382"
     CDS             complement(33176..34099)
                     /locus_tag="J8V14_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA0920517.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34114..34386)
                     /locus_tag="J8V14_000383"
     CDS             complement(34114..34386)
                     /locus_tag="J8V14_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920518.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34856..36481)
                     /gene="groL"
                     /locus_tag="J8V14_000384"
     CDS             complement(34856..36481)
                     /gene="groL"
                     /locus_tag="J8V14_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBA0920519.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36532..36816)
                     /locus_tag="J8V14_000385"
     CDS             complement(36532..36816)
                     /locus_tag="J8V14_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBA0920520.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37041..37703
                     /locus_tag="J8V14_000386"
     CDS             37041..37703
                     /locus_tag="J8V14_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBA0920521.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37779..39071)
                     /locus_tag="J8V14_000387"
     CDS             complement(37779..39071)
                     /locus_tag="J8V14_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0920522.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39241..39870
                     /locus_tag="J8V14_000388"
     CDS             39241..39870
                     /locus_tag="J8V14_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBA0920523.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39973..40782)
                     /locus_tag="J8V14_000389"
     CDS             complement(39973..40782)
                     /locus_tag="J8V14_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA0920524.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40837..41706)
                     /locus_tag="J8V14_000390"
     CDS             complement(40837..41706)
                     /locus_tag="J8V14_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920525.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41707..43023)
                     /locus_tag="J8V14_000391"
     CDS             complement(41707..43023)
                     /locus_tag="J8V14_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920526.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(43020..44855)
                     /gene="walK"
                     /locus_tag="J8V14_000392"
     CDS             complement(43020..44855)
                     /gene="walK"
                     /locus_tag="J8V14_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBA0920527.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44860..45564)
                     /locus_tag="J8V14_000393"
     CDS             complement(44860..45564)
                     /locus_tag="J8V14_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920528.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45754..46614)
                     /locus_tag="J8V14_000394"
     CDS             complement(45754..46614)
                     /locus_tag="J8V14_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBA0920529.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46601..47170)
                     /locus_tag="J8V14_000395"
     CDS             complement(46601..47170)
                     /locus_tag="J8V14_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBA0920530.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47551..47623
                     /locus_tag="J8V14_000396"
     tRNA            47551..47623
                     /locus_tag="J8V14_000396"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47584..47586,aa:Lys,seq:ctt)
     gene            complement(48119..48505)
                     /locus_tag="J8V14_000397"
     CDS             complement(48119..48505)
                     /locus_tag="J8V14_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBA0920531.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48502..49023)
                     /locus_tag="J8V14_000398"
     CDS             complement(48502..49023)
                     /locus_tag="J8V14_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBA0920532.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(49025..50059)
                     /gene="sppA"
                     /locus_tag="J8V14_000399"
     CDS             complement(49025..50059)
                     /gene="sppA"
                     /locus_tag="J8V14_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBA0920533.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50092..51507)
                     /locus_tag="J8V14_000400"
     CDS             complement(50092..51507)
                     /locus_tag="J8V14_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA0920534.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51476..53629)
                     /locus_tag="J8V14_000401"
     CDS             complement(51476..53629)
                     /locus_tag="J8V14_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBA0920535.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53887..54123
                     /locus_tag="J8V14_000402"
     CDS             53887..54123
                     /locus_tag="J8V14_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBA0920536.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54142..54357
                     /locus_tag="J8V14_000403"
     CDS             54142..54357
                     /locus_tag="J8V14_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBA0920537.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55060..55812)
                     /locus_tag="J8V14_000404"
     CDS             complement(55060..55812)
                     /locus_tag="J8V14_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920538.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(55827..57074)
                     /locus_tag="J8V14_000405"
     CDS             complement(55827..57074)
                     /locus_tag="J8V14_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBA0920539.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(57150..57296)
                     /locus_tag="J8V14_000406"
     CDS             complement(57150..57296)
                     /locus_tag="J8V14_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBA0920540.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT        17057 a        11731 c         9561 g        19024 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     8881 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     8941 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9001 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9061 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9121 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9181 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9241 ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9301 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9361 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9421 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9481 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9541 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9601 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9661 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9721 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
     9781 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
     9841 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
     9901 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
     9961 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10021 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10081 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10141 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10201 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10261 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10321 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10381 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10441 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10501 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10561 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10621 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10681 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10741 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    10801 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    10861 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    10921 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    10981 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11041 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11101 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11161 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11221 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11281 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11341 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11401 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11461 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11521 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11581 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11641 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11701 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    11761 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    11821 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    11881 tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc
    11941 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    12001 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    12061 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    12121 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    12181 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    12241 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    12301 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    12361 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    12421 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    12481 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    12541 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    12601 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    12661 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    12721 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    12781 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    12841 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    12901 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    12961 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    13021 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    13081 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    13141 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    13201 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    13261 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    13321 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    13381 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    13441 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    13501 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    13561 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    13621 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    13681 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    13741 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    13801 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    13861 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    13921 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    13981 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    14041 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    14101 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    14161 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    14221 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    14281 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    14341 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    14401 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    14461 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    14521 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    14581 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    14641 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    14701 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    14761 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    14821 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    14881 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    14941 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    15001 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    15061 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    15121 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    15181 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    15241 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    15301 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    15361 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    15421 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    15481 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    15541 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    15601 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    15661 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    15721 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    15781 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    15841 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    15901 aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca
    15961 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    16021 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    16081 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    16141 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    16201 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    16261 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    16321 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    16381 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    16441 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    16501 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    16561 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    16621 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    16681 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    16741 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    16801 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt
    16861 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    16921 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    16981 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    17041 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    17101 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    17161 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    17221 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    17281 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    17341 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    17401 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    17461 taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    17521 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    17581 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    17641 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    17701 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    17761 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    17821 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    17881 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    17941 tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg
    18001 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    18061 tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag
    18121 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    18181 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    18241 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    18301 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    18361 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    18421 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    18481 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    18541 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    18601 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    18661 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    18721 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    18781 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    18841 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    18901 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    18961 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    19021 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    19081 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    19141 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    19201 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    19261 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    19321 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    19381 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    19441 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    19501 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    19561 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    19621 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    19681 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    19741 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    19801 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    19861 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    19921 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    19981 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    20041 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    20101 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    20161 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    20221 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    20281 ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa
    20341 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    20401 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    20461 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    20521 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    20581 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    20641 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    20701 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    20761 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    20821 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    20881 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    20941 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    21001 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    21061 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    21121 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    21181 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    21241 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    21301 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    21361 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    21421 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    21481 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    21541 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    21601 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    21661 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    21721 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    21781 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    21841 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    21901 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    21961 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    22021 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    22081 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    22141 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    22201 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    22261 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    22321 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    22381 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    22441 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    22501 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    22561 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    22621 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    22681 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    22741 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    22801 ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca
    22861 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    22921 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    22981 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    23041 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    23101 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    23161 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    23221 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    23281 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    23341 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    23401 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    23461 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    23521 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    23581 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    23641 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    23701 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    23761 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    23821 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    23881 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    23941 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    24001 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    24061 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    24121 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    24181 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    24241 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    24301 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    24361 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    24421 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    24481 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    24541 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    24601 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    24661 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    24721 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    24781 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    24841 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    24901 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    24961 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    25021 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    25081 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    25141 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    25201 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    25261 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    25321 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    25381 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    25441 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    25501 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    25561 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    25621 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    25681 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    25741 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    25801 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    25861 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    25921 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    25981 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    26041 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    26101 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    26161 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    26221 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    26281 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    26341 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    26401 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    26461 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    26521 gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct
    26581 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    26641 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    26701 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    26761 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    26821 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    26881 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    26941 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    27001 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    27061 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    27121 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    27181 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    27241 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    27301 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    27361 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    27421 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    27481 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    27541 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    27601 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    27661 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
    27721 aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga
    27781 ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc
    27841 tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga
    27901 tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga
    27961 ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt
    28021 cgataaaatc gatattagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac
    28081 atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac
    28141 gatccatgat tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat
    28201 ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt
    28261 aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc
    28321 tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg
    28381 aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta
    28441 tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac
    28501 gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga
    28561 cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt
    28621 atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg
    28681 atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt
    28741 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
    28801 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
    28861 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
    28921 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
    28981 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
    29041 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
    29101 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
    29161 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
    29221 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
    29281 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
    29341 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
    29401 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
    29461 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
    29521 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
    29581 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
    29641 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
    29701 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
    29761 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
    29821 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
    29881 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
    29941 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
    30001 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
    30061 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
    30121 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
    30181 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
    30241 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
    30301 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
    30361 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
    30421 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
    30481 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
    30541 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
    30601 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
    30661 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
    30721 caatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
    30781 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
    30841 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
    30901 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
    30961 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
    31021 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
    31081 gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca
    31141 ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc
    31201 taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca
    31261 atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta
    31321 gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat
    31381 gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt
    31441 gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc
    31501 gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt
    31561 taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca
    31621 tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa
    31681 gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat
    31741 atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac
    31801 atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc
    31861 ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc
    31921 taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt
    31981 cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat tactattcac
    32041 aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt agatccaatc
    32101 ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag
    32161 acaaaaggat ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac
    32221 aattttttct tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa
    32281 ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg
    32341 cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg
    32401 atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca
    32461 ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct
    32521 ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca
    32581 actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa
    32641 tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt
    32701 cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat
    32761 aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt
    32821 tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag
    32881 tggttatctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg
    32941 tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca
    33001 aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa
    33061 gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa
    33121 cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc
    33181 gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa
    33241 atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc
    33301 tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca
    33361 agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa
    33421 aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt
    33481 catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta
    33541 gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag
    33601 taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct
    33661 tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg
    33721 tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat
    33781 accttcgctc tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat
    33841 caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag
    33901 gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag
    33961 ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat
    34021 atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga
    34081 tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata
    34141 gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact
    34201 gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat
    34261 ttcttttact atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt
    34321 gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt
    34381 attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc
    34441 aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt
    34501 attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga
    34561 tatttacttt taatagataa aatattataa ctattaaaaa taactatttt cattataatt
    34621 acacacaaaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    34681 gtataaacag cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    34741 gaaaagcaat ttccgtgaat ttcttcttat ttcttggagt taaacgcttc tgttccagcc
    34801 tctttttatc tgtctttcac taatgatcag ttgtagataa gcaagttgct ttttcttaca
    34861 tcataccgcc catcattgat ggatccattg caggagctgc tggcgctgct ggttctggtt
    34921 tatctgcaac gactgcttct gtcgttaata acaaggcaga aacggaagcg gcattttgca
    34981 aggcagaacg tgttacttta gttgggtcta cgatacctgc ttcaaccatg tttacccatt
    35041 ctccagtagc agcattgaaa ccagtgccta gttccacgtt cttcaattta tcaacaatca
    35101 ctgatccttc ataaccagcg ttttcagcga tttgacgaat tggttcttct aaagcacgaa
    35161 caacgatctt gatacctgtt gccacgtctc cttctgcttc tacagcagaa actttgctga
    35221 taacgttcac tagagctgta ccaccacctg agaccattcc ttcttctact gcggcacgtg
    35281 tagcattcaa tgcatcttca atgcgtaatt ttaattcttt caattctgtt tcagtagctg
    35341 caccaacttt aacaacagct acaccacctg ctaatttagc taagcgttct tgtaattttt
    35401 cgcggtcaaa atcagaagtt gtttcagcaa tttggttttt gatcaattgc acacgagctt
    35461 cgattgcttc tttttctcca gaaccttcta cgatcgttgt attgtcttta tctacaacaa
    35521 ctttagaagc atttcctaag ttttcgattg ttgcatcttt caattctaaa cctagatcgt
    35581 cagtaattac tgtaccacct gtcaaaatag cgatatcttc aagcattgct ttacgacgat
    35641 cgccgaaacc aggagctttt acagctacta cgttgaatgt tccacggatt ttgttcaata
    35701 caagagttgg caatgcttca ccatctacat cgtcagcaat aatcaacaat ggacgtgatt
    35761 gttgcaagat ttgttctaac aatggcagaa tatcttggat gttagagatt tttttgtctg
    35821 tgatcaagat atatgggttt tctagaacag cttccatttt gtcgttatct gttaccatat
    35881 attgtgataa gtaaccacga tcaaactgca taccttctac aacatctagt tctgtttcga
    35941 tcccttttga ttcttcgatt gtgatgacac cgtcgtttcc aactttttcc attgcatcag
    36001 caatcaaatg tccaacttta tcagaacccg aagaaacagc tgctacttgt gcaatggctt
    36061 cttttgaatc aacaacagtt gagatattat gcaattcttc gactgctgct tttgttgcca
    36121 attcgatccc gcgacgaatg cctagaggat tagcacctgc agttacgttt tttaaacctt
    36181 cgcgaacgat tgcttgtgtc agaacagttg ctgtcgttgt accgtctcct gcaatatcat
    36241 ttgtttttga tgctacttct gatacaagtt ttgcacccat gttttcaaaa tgatcttcta
    36301 attcgatttc ttttgcgatc gtcacaccat catttgtgat caatggagag ccgtaagatt
    36361 tttctagtac gacatttcgg cctttaggtc ctaatgttac tttcactgta tctgctaatt
    36421 tatctacccc gcgtaacata gctgcgcgtg cgtcttctgc aaatttcaat tcttttgcca
    36481 tgattgattc acctcattga ttatttttga ttattttttt atcttccaaa tttactcaac
    36541 aatcgccatg atatcttttc ctgcgacgat cagatattct tttccttcgt attttacttc
    36601 tgtaccggcg tatttttcga acataacttg gtcgcctgct tttacagcgg caggaacttt
    36661 ttcaccgttt tctaacaaac ggccttcacc gactgcaaca actgtcccag tttggggttt
    36721 ctctttagca gcagaagcta aaacgattcc tccgacagtt ttttcttctt cttttgcgac
    36781 ttcgataatg acgcgatcac ctaatggttt taacacgata aatccctccg ataactcttt
    36841 ttttaatctg ttagcactct taaccttcga gtgctaacct acatttttaa taatactcat
    36901 tctccctctt ttttgcaagc ttttaaatca aagaaaagaa attttattct gacagctgct
    36961 tattgaaatt atgatataat tgaaatcaat cgatataagc ttgccttgca tgcttgttat
    37021 cccttaaaag gaggaattac atgtctttaa aaaaatatag cttcctcagc attttttgct
    37081 atggtctaat ttttatctca cccttgatcc tagcttcgat aggacttgta aaatctacgt
    37141 ctgaattgat cacagcaact gcagtcatgt atatactagg cgccgttgtc ctagctattt
    37201 tttattttaa gcaacgtgaa cccttagcga tcgaatcagc ggtaaaaaaa gcttctgctc
    37261 caaagattgt tatttatgga ttggttggaa tctttgtcgc attgattctg caaagcatcg
    37321 ctgtcatgtt agaatctgta ttgtttggca aagcaacccc ttccgagaat acacaaaata
    37381 tcatccagat gatcatggaa gcacccgctt ttattttggc gacaactgtt gctggaccca
    37441 tcatggaaga gttcgtattc cgccgcagta tactggggat catcagtcga tattcaaatt
    37501 tttgggtcgg agcagtgatc agctctttgc tgtttgcgtt tgctcataat gatggtcatc
    37561 tgctaatcta ctttttccta ggtttcttct tcagcctaga gtacaaagca actggtcgga
    37621 tctggacatc gatgatcaca cacgtaggaa tgaacacatt agttgttctt gttcaattag
    37681 ctgtccaaaa agggctagta taaacatgac tataaatact cctgtcatcc tagattggtg
    37741 attttcccaa tctaaagcga caggagtatt tttgtttttt agtcgttcat cttttctgct
    37801 gctccatcat tttttgtctg tctttccttt ttgcagtttg cagaatctat ttttttcaaa
    37861 aatgggaaat aaatgaaaaa actaattgac aacacgatac cttgtaaaat cgctgcaggc
    37921 actccgccta ctaagaaacc cgagaggatc ggcggtgttg tccaagggac ttggactgct
    37981 gtaaataaag gaacgaatcc aaaatataaa gcggaatagg taatcaatcc tgaaacgacc
    38041 ggtgcgaaaa taaaaggaac agccatcatc gggttcatca cgatcggtgt ggaaaaaata
    38101 attgattcat tgatgttgaa aaaggctggt aaaagagcaa gatttcctaa ttctctgtat
    38161 ttcttcgatt tcgcaaaaac cgtcataaaa aatacagccc ctaacgtcaa tccagcaccc
    38221 gtaaccgtca tgaactgatc gagaaattgc tgagtaacga tatgtccgcc atttgccacc
    38281 gtcaactctt gtcctgaggc taagatttct gcatttgcta atgaattcgc ttgcaagacg
    38341 ggactaacca acccattgac catcgcagat ccatgaagac caaataacca taaaaacgga
    38401 acaaaaaaac ctaataaaag cacaccaaac aacgtgtcta tgaacgattg aatgggaagc
    38461 tggacatatg tccatactcc ttgaacaaca gtcattctga acaaatgttg aaaaaccgct
    38521 gaaccgataa gcgtaaaagt gactataaca atgattggaa tcaatgaagt aaaagactgc
    38581 caaacatttt caggtatctt ctctctttgc ttccaccctt tcgcaagtct tactaaacgt
    38641 gcatagcccc atccgatgat gagcccagtt gtaactgctc ctagtatccc ttcagcacct
    38701 gtccagacag tatctatctg agtatttagt ggctgaataa gcagtagata agcagacaag
    38761 gctgttatgc cgactggtaa gcgatcgatg tcttcttctt ttgcataagc gtatgcaatg
    38821 gcaaaaacga tccaaaaggc tatcacatca aatgtagcct ggtatacttg tgtaaaatac
    38881 atagaaaatc ctgttttgtc tatccactga gcgatgtaat ccacaggaaa atgagcaaat
    38941 aagaaaaaca gcgagccaac aataattatt gggatcgtgt aagtcattcc attacgcaaa
    39001 gcagttaagg gtctaagatt cacaacagat gttatcattg gtaaaaaggc acgatctaac
    39061 cactctttca tttcttatct ccttcaactt ctatttttat caatcagttt tcttaattta
    39121 actataccca atgatcacga accatgaaag gaatgcgcac tgagcttttc aaaaatcgga
    39181 ttctttgacg aatattcagc aactgcttac aatggacagg aataaaaaag gaggtttttt
    39241 atgaaaattg cactcattgg agctactggc catgcaggtt catttatttt agacgaagca
    39301 ttgtccagag atttagacgt gacggcaatc gtgcgtcatc cagaaaaatt gccatcagat
    39361 gttccgttta tacaaaagga tttatttgag ttgacaacta ctgatcttgc atcttttgat
    39421 gttgtcgttg atgctttcaa cgctcccaaa ggaaaagaag aaatgcatca aacttctttg
    39481 tcccatttga ttgagatgct tgaaggaacc gatacacgct taattgtcgt tgggggtgcc
    39541 tcttcgttat tcttagatac agaaaagacg actcgtatga ttgaccaagt acctaaaaat
    39601 gcacccttct atccaacagc tttcaatatg tacgaaggat tgttaaaatt gaaacaatct
    39661 aaaaatctac attggactta tatcagtcca gcttctttat ttgatccaca tggcaaacgt
    39721 acaggtactt atttgttaag tgatgattat ttacaaagaa acgctgctag cgacagtacg
    39781 atcagcatgg cagattacgc gattgcatta gtagatgaga tactggatga gaaacatgaa
    39841 aagcagcaca tcagtgtagt cagtcgataa tacggttatt tttttaatat gctaaaaaaa
    39901 taaaaataga agataaaacc aaccctgatt gattttatct tctgcttttt atatttttat
    39961 tgattgaact gtttaaatcg aaaatagctc agttgcttca tctggatctg tatcatagat
    40021 ttcaaaatca tgtccaacgc caaaatcttt ttctttcaaa acattttcca ttgttaaatg
    40081 agcaagttct ttcatattgt tttccttgct aagatggcca agatagattc gtttggtccg
    40141 attaccgatc acatcagtca tgacaagtgc accgtcttcg ttggaaagat gtcctctatc
    40201 tcctaaaata cgctgtttta aattccacgg ataaggtccc atgcgtaaca tctcaagatc
    40261 atgattactt tccatcaaat aaccatcggc attttcaatg acacctcgca tataatcact
    40321 gcaatatccg gtgtccgtca acatgacgaa tgaccgattt tctttatgga aacgataaaa
    40381 ttgcggagcc gctgcgtcat gggagacgcc gaaactctca atatcgatat cgccgaatgt
    40441 tctgactttc cccatctcaa atagatgttt ttgttccact ggtacatttc caatcagtgg
    40501 ttccatcgct tcccaagtct gctgattggc aaaaacatcc aaatgatact tacgagccaa
    40561 gacacccacg ccatgtatat gatctcgatg ttcatgcgtc acaagtattc catctaattg
    40621 gtccggtgtc cgatcaattt gagccatcaa ggaagtgatt tttttaccgc taagtcctgc
    40681 atcgaccaaa agccgttttt tttcactttc gatataaagt gaattacccg aacttccact
    40741 tgctaggata ctgattttaa aagctggttt ttcttcagcc atttgactcc tacttccctt
    40801 cttactttgt aaactttttt cattatactc gcttttttat tgatcttcca cttttggtac
    40861 agtattgttt gtcacgatcg tattgctgaa agcgttgacg ctctcaattt ggaccacttt
    40921 atcgcttgtt tcgatcgcta catgccaaac aggaacatac acattttttt cccggacttt
    40981 cagaatacag gaataagcta gctgacgcca taaaattttt gatcctcttg gtattttgtt
    41041 attgatgtac aatgtattga ttgcttcctc ttccgtatac agttccgtct tttcgcgcag
    41101 ctgctcgata tcagataaat gcgtctgcat gtatttcgtg atgctataat cattatcgtt
    41161 tttttctaaa gaaagaacga tttttgctgt atcatcattg aatgggatgc cttcatactc
    41221 ttggcttgct tggatttcta gtggttcttc ttctaaatga gaaaagtttt tcgtgtacac
    41281 ataatcatta ccgaataaaa tattgtcttt gttagataaa aaatcttcca aggaactact
    41341 aattttatct ggatcgatca cgttcttttc agcatctgaa ctggataaag atttcgtcaa
    41401 aacatcaccg tctaaaaaca caccacttgc cagcaagccg cttttaccag ccttgcgata
    41461 attcgctaat gaagtgctaa gattggtctg ctccgcgcta agataatagc cttgcttgtt
    41521 ctcagacgaa atcttccccg tgtaagtgat atcgtcgcta gccagacgtt gttggattga
    41581 ttcagtctga ttagagcgaa tgacattgtt ttcttggtat accgcgtcat gataactgct
    41641 gaataaaaac acattcaacc ctaaaaaagc gacgaaaaat atccattcga tttttttgaa
    41701 atccaattaa ttcgcctcca atccaggtaa tttttccaga aggtcgttag ctgaatacca
    41761 gttatcttta tatttcacat accattcagg taagagatca acgacacgat tcgtttcttt
    41821 gacattttgc catgtatagc caacgatcac tgaaccaatc ttgttgctat ctgctccctt
    41881 agcttttaac tgctggatga tttcattcgt acttgcaagt tctacttctt cgtctgaagg
    41941 tatcggcact tgaatcgtat tcaaactggt ttggatattc acttgcacag aaccattcgt
    42001 ttcaccgcca acttccacac caatctgtcc tctgctgtct gtcccaaaaa ccgggaagcc
    42061 ttccacgaat atccgataat cgatcgtatt gtcactgcgg tcaaaatatc tgaggttccc
    42121 taaagaactg cctaatttgc taatataggg gaaactttca gaaaatacac tagcattttc
    42181 ttttttctct ggcagctctc cacgaaaatc aactacttgc gtttccgaat ggatagacat
    42241 tgtttcattc ccaccgtaaa aaatcaattc attgctttca tcattatttt tcacgctatc
    42301 tggtttctgg aaaaaagcat ttctaaaaag tgtatagggt tgctgtgaca ggatataact
    42361 atacttcttc agtttgatcg aatgtttcgt agcatattgc gtgtcgatcg tcgatggttc
    42421 tgttgtcatt tctgtccatt ttgtgtcttt tgctgttaat tctttattga tatctgtcca
    42481 attcaacgaa atatttgctt catatacgag atggttcgaa taattcaaaa atcggatttt
    42541 tttatttcct tgatcgaatt gtattctagt aaaatacata ttttcgcttt cttttgccaa
    42601 gttcgacaca tctaaatcta aatcaaatgc ttccttgtat tctcttagca aaaaaggcgc
    42661 attataggcc aactcaaaac cagagttcag atttttcacc tgttcaaact gtgatttgtc
    42721 tcctgaaact acttcagaaa gctttccaaa tcttgcttta tcgatgatca attgcaatcg
    42781 gtacacgaga ttttcactgt tggtttcttg gatcattccc gaatggtgcc atgtaacatg
    42841 aagcggaaga aagacttcgg aagtttttcg attgctttga gactcgatca tttgagtgtt
    42901 tgtctcatct acgttgattg aactttttcc tgctgggctc agccagattg cataagagaa
    42961 gtatatgctt aacgcaacca tcaaaatgag tcctatacga acgacttttt cagagattct
    43021 cattcccacc attcctcctc ataaggttca tatggcagcg agatataaaa agtcgaccct
    43081 tttccttcct tgctctctgc ccaaatagcg cctcgatgcg ccttaatcac ttctctagaa
    43141 atagctaatc caagtcctgt cccaccttgt gctcttgcac gtgctttatc tactcgatag
    43201 aaccgctcga acactcggtt cagatctttt ttaggaatcc ccaatccttg atcggtgata
    43261 cttaaaatca cgttattatg cgtttctaat aagcgacaag taatcgttcc cccatctggt
    43321 gaatacttga tcgcgttatt catgatatta tcgactactt ggatgatttt atctgtatct
    43381 acttcgaccc aaagatctct ttgcgtaaat tcacgacgga tcttataatt tttttcctga
    43441 tttcccacca tcatatcaaa ccggtcaaga acaaaattca ctaactcgtt gaaattgaca
    43501 tattctaatt gaagctgggt attacctgta tccatgcgag atagatttaa gaggtcattg
    43561 atcattcgaa tcattcggtc tgtttcatct agcgttacct tcaaaaaatt tggtgctact
    43621 tcctcgtctt tccacgcacc ttcgctcaat gcttcgatat aactacgcat acttgtcaac
    43681 ggtgtacgaa gttcatggga aacattagag acgaattcac gacgttcccg ttctgttttt
    43741 tcttgttctg tcacatcatg caagacagct actaatccgc tgataaaacc tgattcgcga
    43801 cggatcatag aaaagtctac tcgcaagatc atctgatcac tttccaatgt actggttgaa
    43861 cggtcaagta ccatttcgtt tggatcttct aataatttcc gcaacgtata ttcttcttcg
    43921 atatctaaaa gtgtcaaaat cgattgtccg attgcttcct cgtttttgat atctagcaag
    43981 gaaacagcca tatcattgat cgtaatgact ttacccctgc gatctgtcgc aatgacgccg
    44041 tctgtcatat gggatagcac gctatccaaa cgattgcgtt cagcttccat tgtatcttga
    44101 gcttcttcaa tacgttcagc taactgattg aaggtatcag ccagctgtcc aagttcatct
    44161 tgtccatgca ctttgacttt ccgactgtag tctcctctag cgattcgaat ggcttgttct
    44221 ctcatttcac cgatcggctg tgtaatcgaa cgagcaacta aaacagatac gatcattgaa
    44281 ataaatgccg caattagtga ggcagtaaag aaaatgactg ctgtgttatt gatttccgaa
    44341 tatttttgtt cgatattact tttgacgtaa agagccccga taacggcatc gccggtcggg
    44401 gacaggatcg gttgaacatt gatattcacc cgtttatcgt cgttatctaa agcaacataa
    44461 cgtttagtcg tgaaatcatt gatatcaaca taatcatttt tctttcccac agcgctttgt
    44521 tcattgacat cggttgttcc tcggatgatc cctttttcgt caacgacccg tatttcgatg
    44581 atatcgctag tcgcactgtt ttcaataaat cttttgatat tcgcatttac ctcttcatca
    44641 tctgcattat cttttcgatt caattccggg ctgattgttg ttgctaatga ttccaccgtt
    44701 tgattcatat ctttaataaa tgtattgatc gtggaacgtt ctaaaccacg tataaagtag
    44761 gcaccaataa tttcgatcga gatcaataaa atcagaataa aggtaatagc gatcttaaaa
    44821 ttgactgatc gaaaaaagcg gacttttctt ttcatccaat tactcctgtt ctgggttacg
    44881 caaatagtaa ccgacaccac gacgtgtcac taaataattt ggatggcttg gattatcttc
    44941 gatcttttca cgcaaacgac gtacagttac atccactgtt cgtacatctc caaaataatc
    45001 ataaccccaa accgtttgta ataaatgttc tcttgtcatc acttgtccaa ggtgcttcgc
    45061 caagtaaaag agtagttcga attcacgatg tgttaactcg attgtttctc ctcgtttcgt
    45121 caccatgtaa gcttctggat ggatcgtcaa atcaccaatc gtcaattccg caggtgctgc
    45181 ctcttctggt tccttcgcag ccgtggcacc gcgacgaaga tttgctttga cacgtgcaac
    45241 taattctcgg ttggaaaatg gcttggtcac gtaatcatcg gcgcctaact ctaagcctag
    45301 gactttatca atttcagaat ctttggctgt taccataatg atcggcatat cataagtttt
    45361 gcgtacctca cgagcaactt ctaagccgtc cattttaggt agcatcaaat caagtaaaat
    45421 caaatcaggt tctacctcag caactttttc caaggcttct tccccatcat atgcagtata
    45481 aacgtcatag ccttctttgg ctaaattgaa tttgacgata tccgaaatcg gcttctcatc
    45541 atcgacaact aaaacttttt tcataaagag ccacctctta tatattattg tcataccttc
    45601 tcattatacc gtatttctgc ttttgtttca tctttgccta taaaacaaaa gagaaaatta
    45661 aaatgaaagt gcaaaatact ttattatttt ttgttctaac ttcaataaaa aaggaaaaca
    45721 ctgattttca cctcagtgtt tccttttttt gatttatcct tctaaaactt gtacaccgac
    45781 acccaataat cccggaccag tgtggactac taatgccgga gaaatctggc caaagtagat
    45841 ttggtttgct tgtgggaact ctcttttaag tctcgcttcc atttcaattg cttcttcttt
    45901 cgcatcccca tgggcaacag ctaagttgaa gacctttgca ttaccgattt tttctttgac
    45961 gtaagcaacc gttttatcca aacttttttt acgtccccta gcttttccta ctgtgtaata
    46021 aatcccttct tcattacatg agatgattgg gttaagcttt aaagcggttc ctacaataga
    46081 agcaactaaa ccgatccgtc cacctttttg aagatattcc aatgtcgcaa cattaaagaa
    46141 aacttttgtt ctttcgacta actcattcag attactgatc agttgatccc attctactcc
    46201 ttcttccagc cattttgctg cttggatcgc agaaaaacct gcaccaatac caatattttt
    46261 cgtatctagt acatatgctt ccaattcttc ttgctgttgt gcaattaaac gaaccacatt
    46321 atatgtcccg ctcaatccac tggaaattgt taccgcaagt accttttcat aaccgtcttt
    46381 tttgatttgc tcaaagattt ctgtgatcga ctcaccatcc ggcaaagatg taccagggat
    46441 ttcctctggc aaacgttgat agatctcttc aggggtaatg tcaattttat ctgtatatac
    46501 ctgatcttta taaataattt tcaatgggat catatacatc tcgtattttt ttatcaattc
    46561 ttctggaaca tccgtacctg aatctactaa aattgcgatt ttattgtttt tcatttgttt
    46621 tctcctcttt taaatattcg gtttctagaa caatcgtttt ttccgcaaga agtttcattg
    46681 caaacgataa agttgccgct cttacagcaa tatattctac aggggttgcc tggtcgaatt
    46741 tttgcggatg agatttacct tgtgctaatt ggctgaccat ccacacagct gcttcttgtt
    46801 catcgcaaaa tagattatag gcttcacgaa ttcctactgt ttttccttgg aacaatatcc
    46861 cttctttgat ttcaggaata gtcaatactt gtttcaacgt agtgattgca agtaaaaatg
    46921 ctacgtgttc tttgttatat ctttttttaa ctggtgctgg tatcaaacct agtttaacgt
    46981 aattgtttac catagaagag gtaagcaatg gatgcttttc tccttgtatc acaggtgaaa
    47041 gataacgatc aactaacgtt ctgacttggt ccatataaag ttctaagtca gggagttctt
    47101 cccagtgagg cagacggaat gtttctagtt ccttccccca ctccattaat tgctgacgca
    47161 ttttttccat cataaccgct ccttttttca tttcataaac atgatatcag acttatgata
    47221 aacattcaat ctagttgctt taactaggtg atttgattag cgggattcca tgaaaacaaa
    47281 aaacaagaag gctaagcctt cttgtctttt atcgttaaga acaggagacc cgccggggag
    47341 acggcgggaa aggagtaaaa aatgaaaaaa gtgttagggt ttgttctctt gcaagaactt
    47401 tgtttgttgg tatgcttata taatagcgag caaatgtgaa taatctatga atgatttcct
    47461 ataaggaagt aaagattttt ctgattttct ttaaatgtag tcttgcattt taaaattctt
    47521 agtgctacta ttattaagta ctttttgtac gggccgttag ctcagttggt agagcagctg
    47581 actcttaatc agcgggtcgc gggttcgagc ccctcacggc ccattgggtg ccaaacccac
    47641 ggaacaggta aatgttggtg cgaaagcatt tcgaaaacat ttacgctgtt ctttttttat
    47701 ttttcaaaat aactagggct ttttattttt aactcaaagt ccactgtagc cctttggact
    47761 ttgagttaaa aaagaagcat aattatgaac agtaaagttt ctaatataac aataattcga
    47821 gaagctttgc aaaatcatgc tgccaatgaa ctctctaaac atataggatt aaatctaagt
    47881 actataaaag gttaaaaagt gaagaatatt taatagaaaa actaaattta cttgctgctt
    47941 tccccttttc cattaataca acaatcagag agtgtctttt tttcgaaaaa attagaataa
    48001 ataaaagaca cgtagttttt ttatctacat gtcttccatc gactacaagt tatcatttct
    48061 caaaacgtat ttagctttta attgattaat cgtttcacgt tgtctacaca ttgcctaatt
    48121 aagattttct tattagaagc atcaatcccg caataatacc taaaactcca ggaattaccc
    48181 acaataatgt taaaagaaaa aatggaataa tagataaaat gagtaaacta ataccataga
    48241 ccactgtttt ttgatttttt cttactaaaa aacctattat gaaaatcatt atcgaaaaaa
    48301 ctagggtaat tcctccaaat aaaacaacat tcccgcttag attgactaac atatctatat
    48361 ccttactggt tagttctgta gtatttaaag tagttgaatc aatagaagat agaaacatac
    48421 cacccatcaa tgctaaaaga ctacataaaa tccctgttat tccactaatt aaaatcaaaa
    48481 taaactctgt ttttctctta ttcatttaat tctctccaga ttaagtgttt tagaattaga
    48541 aattttttga ccatagttaa tagcttctaa tgaacgaata acaaccactg acgtgttaga
    48601 aaaataatcc ccaatatgtt gtttcttggg cgtgaaagca gccaccaaat acccaatttg
    48661 aaatacaaaa ttttttaata taaacctaca tatacactca cgaataaaga tagtttgcca
    48721 acttagctct ttttcctcaa agctcactac tcttatccca aaaatcattt tcccaattgt
    48781 ttgtcctgaa ttcagcttag tcaaaagtac aaaataggtt aaataaatag ctaatgacgc
    48841 tataccataa aaagtaataa tggatttgtc agattccagt cccatgattt tgtaaaagag
    48901 acctatagta gcatgggtga tgatgctaat acaaattaaa tcaataagaa aagcaaaaaa
    48961 tcgtaaccaa aatcccgcaa atacataatt aggcaaagtt tcaatgccac tcttcttatt
    49021 cattttatag ccctccatag taatacattg gttttggagt atcagaaaat ccaagttttt
    49081 caaaaactga aattacacga tctgatgtag ttgcctttag cccttgccat tcagcaatct
    49141 tattacctaa ccaagaagaa gcaaagccag ttgtgtcatt tttatattct ataaccgtcg
    49201 catcagttaa tttcttctct ttttttaggg aatctaatgc atcttcagag taaccaattt
    49261 gatccaccaa cccattttct actgcttggt taccatcata aattcttcca tccgcaagtt
    49321 ctttcactgc attagtatcc atatttcttc cttttgctac aacattgaca aaacgatcat
    49381 aagcactcat cacaaattcc tgtataactt tattctcttc ctctgaaggt ttgtgttgag
    49441 gtgacatcat atcttttaat gcaccacttt tatacgtcgc atcagttacg ccaagttttt
    49501 ctaataagcc agaataattt aaaccagaaa taatgacacc aattgatcca gttgttgttt
    49561 cttccgtagc aaatatttta tctgaaccag cagaaatgta gtaccctcca cttgccgctg
    49621 tatttttaaa tgcagtatag ataggaatat ctagcttttt aatttttgcc atttctttag
    49681 caatctcagc actttcatat attcctcccc caggtgaatt tacttctaat aacactccct
    49741 taaccgcttt gtcttcttga attttcttta gctgtgttaa aaaattctga tggttatatt
    49801 gttccctact aaaaagattg ctttcacctg tatctgcaat aactccattg acggatagtt
    49861 tgacaatctt tttatttgat gcgccttctt ctaacgtttc ttctgtgatt tcgttagatc
    49921 cgtaaaaaat cttatttatt cctgttaggg ttgtatttga tgcttcctct ttatcttttc
    49981 ctggaattag cgcgacgata atggatagta acaataatcc gcacgctact cccacagcaa
    50041 tccatcttct tttattcata taaactcctc ctatattctt agtaaaaata attatttttt
    50101 atttagaaac acatcgacaa tttgttgcca atatgttttt tctcctacaa ttaaagacaa
    50161 ctctccagtt aatccataac gaaacggaaa cgattgagtt gtttttaacg ttcctttcac
    50221 tgcataaaaa acacctttct cagtggcagt agaattttct gaaatttcat ctaaagttcc
    50281 gtcgatcgta atcggagccc cacctttttg atccaattta aaatgaacct tcatcccttc
    50341 tttaatatag gtactttcat tcgcaggtaa aagtgctgta aatttcattt ttgatttttc
    50401 gtggatcgga taaatttctg caagcactgt tcctttggct agttctgctt gtccctttgt
    50461 ttcttcattt aaatgaaccg ttccagatac aggagctttt agtattcctt ttgaaagttg
    50521 atcgtccaaa cttttgataa ctgactggtt cttctcttgt ttttcattta gctctgtaac
    50581 tgtttgtttt acagttgtta ttgcttgttc tagctcttgc tttccttttt ccgtttgact
    50641 aattctgtca ttctcagaag tagttggttt aactaattta gctttttctg actgatactg
    50701 ttctacttct ttttttagtt gctcaatttg ttcatttatg cttgctgaaa ttgtcagttt
    50761 cacttgattt ttttgatcat cagaaacatt atttagttgt tcttgccaat tctcatagtt
    50821 tgccatcatt tcttttgaaa aatcttttaa tgattgatta ttactccaag ctacctgtac
    50881 ttgttgccat tcttgtaatt tactatctga actctcaatt tgtttttcta gttgtctttt
    50941 tgtcttttcg tatacttcta attgcttttg atcatttaat tcactttgct tcaaagcgta
    51001 gcggagtgat tctttttctg acaacatgct ctttaattga ttactgtagc caaaactgtc
    51061 atccgttgaa aacaaatttt ttcctttact tatactttca ataaatagct gagccatctt
    51121 tatttgttga gtaatacgtt ggttttcttg ttctatctga gccttttcat tctctattaa
    51181 agaacaatcc aatctaacca acacatcgtc cttatgaaca aattgatttt cttttaggaa
    51241 gttttccatt attttagctt ctaatggcac ttgtaacttc tctatttttt cggggttcaa
    51301 ttgagccgtt gtccgaataa caacctcttt tttagcaaag gttaaaaaca atccaataat
    51361 acataaaaaa agaattacag ggtataacac ccaacggtag aagctatgat gttgtttagt
    51421 atagatacta gaatgttcga accaattatt attctgcata aattgtttcc ctctcctaga
    51481 tttcccataa tttttgatag ataccatcgg caaaacgaag ttcatcgtgc gttccttttt
    51541 ctaccaaatg cccttcatgg agaactaaaa tttgatcaca tgcttttgct attggtaaat
    51601 gatgcgcaat aaaaataatc gttttttctt ttaaattgag gagataattc ataattgcat
    51661 gttctaacaa ggtatctaat ccactagttg cttcatctaa aattaatatg tccggatttt
    51721 ttaataaagc ccgtgctaag gctaatcgtt gtcgttgccc tcctgaaaga ttatttccac
    51781 cttcttccaa caatgtctcg taacgtaatg gctgttgatt aataaattca tttagttgga
    51841 ctatctcaca aacttctatt atttgttcga aacttggtgg gctatctaaa ccgaatataa
    51901 gattctcaat gattgttcca ctaaagaaaa atgactcttg aggcaaatat gtcactcgat
    51961 cgcgtaatat ctgaaaagga atgtctaagt gattaatgtt tccataatgg atcgatcctt
    52021 ctgatgcttc ataaaaacga acaaataact ttgcaagagt tgattttcca gaaccactaa
    52081 ctccaactat agccgtttta ctgtgcgaag gaattgtaca tgaaatttcg ttcaacgttg
    52141 gggccttcat gctatacgag aatgaaacgt tttttacagt gatatcttgt tgaaataatt
    52201 gttcagaaag catatattca aaagactggt tcgatttttc tgaatcaatc gcgaaaattt
    52261 catttaaacg attatttgca acttgagccg tttgcatttt cacttgtaaa ttaataatgt
    52321 tttgtaaagg atctgtcaaa aatactagta aagcattgta tgtgataagt tgacctagac
    52381 taattctatt atccataaca aaataagaac ctagccataa aattaatgca ctgctaatta
    52441 attgaatcaa atgtttaatt ccttgttgaa tattatcaag cgtaaccgta cgaaatgatt
    52501 ttttcattaa tttaacaaat tcatggtcta tacggttata tactttctct tcaccgctat
    52561 atgctttaat tgtttctatt cctttaagac tttctatgat actagaattt acagctgcac
    52621 cggctgccat ttcttccctg ttcgattttt catagctttt cacgaaagcg aatattgcaa
    52681 ctacataaaa aggcaaagag catagagtga ttaaaaacaa cgtactattc tgaatagcta
    52741 acgtaacccc aacaagcaac accataccaa tatctaaaaa tattgataac gtagcacttg
    52801 ctaaagcatc aataatctta ctggcatcaa gaaaacgaga aataatttcc cctgattttc
    52861 tcgtagcaaa aaaattcatt ggtaaagtta acacatgttt gaaatactga agcataattg
    52921 ccatactcat tcgctgtcct aaaataataa gaagataact acgactatat tcaaacaata
    52981 cccgaaataa atagacagcg atcaagccaa tggaaattat atttaaagtt gaacgtgctt
    53041 gattagggat gaaaaaatca agaatacctt gaaaataata tgagcttcct atgccaaaaa
    53101 acgtaatgaa aatcgatgct aaaacgatgt ggaatactaa tcctttttgt ttccaaatta
    53161 taggcaaaaa agaacttaat ccagctactt tttctttaat aggaatataa gtttctctag
    53221 gcataggtaa taaaagaata cctgtccatt ctttttgaaa cgcttcaatc gtttttctat
    53281 tttttccatt agcgggatct gcaatccata gtacgtttcc cttcgtttta taaattacaa
    53341 cgtagtgcat atagctgtta tcaaccaata tatgtgcaat taatggtaag ataagatctc
    53401 tttctttcca gatctcacta tctgcttgaa tagccataca atcaaaagac aatttttcaa
    53461 atgtcttttt caatccaaat gctgaagtac cctctaaatc agtcccagat aggtcccgta
    53521 atttatgaat cgggatatca gttttaaaat attttaaaat catcgaaagg caagcaacac
    53581 cacaatcttt ttcatcttgt tgctgcacaa aaggaaatga ttttatcact tatctaacac
    53641 aactccttct aaaaaatttt tatagaaaaa ttctcaatat agctttaata cagtgaaaat
    53701 attgtctcat aattccacaa aaactttgaa aacagttctt gaacactaac tttttcattc
    53761 ctgaatcaaa tttattttgg aaattttact attcaagaat aaacttgata agtttcagga
    53821 agatttttac tgttaatcaa atacgtatgt tacagtagca ccgtaaaaaa tattatttaa
    53881 agaagcgtgt ttaaacatgc aaaatgtaaa ggaactaagt attgtggaaa tgcacaaact
    53941 gttggtggag gcaataatgc ttggggaaaa ttggggcaag ttgtaggtgg acttaccaca
    54001 ggggcagtag gtggtgcagg tttaggaaca gcaatttgcg gaccagcttg cggtgttgtt
    54061 ggagggttat atggagctgt cgctggaggt gctgcagctg gctgggatgc tcgaaaaaaa
    54121 taaaaaagga gaataagtag tatgcatatt aaaaacacaa aaacaacatt tatattatct
    54181 tcagaagagt tgaaaaatat tcaaggtggg agtgccgtgg gagttttagg cactactttt
    54241 tctggggcaa cagcaggagt aaaactttgt tcagccggag gaccttatgc aatagctgct
    54301 tgtggtgtag gtggagcttt attaggtgct ggattcagca tgtggaccgg tgcgtaaaca
    54361 attaaaaaat atcaaacctc aagattggtt tgtgtcgatt acgaacatag gtatgttctt
    54421 tatcttttca aaagtttgga atggacagaa ctttttacta aagcttcttg tgctagtttt
    54481 tataattata ttaaattttt atcttttatt aaagtcagat aaaaattctt tggaaatgta
    54541 atggtagaga tatttttaca aaaggacttg cagcgctgta gttggtgctg caggaggacc
    54601 tatttcggcc gttacaggag gtattggtaa tgtacttgtc ggatgtgcat taaagcctgt
    54661 tcctgcaagt taaatttata aaaacaggaa agtaattatc tcaaatccta tcaagtacaa
    54721 tcataggatt gcacttgaca ggacaaattt tttaactaaa aaatgaaaaa cactttctaa
    54781 aaattagtag aatttgccaa atctttcgca tcttccacca tacatttaat tttaattccc
    54841 ctttatggta cttaataaaa acaagaaaag aactattcat ttggcccttt tttggttcca
    54901 gaccatagaa atattcctcc agtaactaat attgctgtta tgcacgtaac taataacagc
    54961 tataaaaatt ggtacaactt taataggatt cagaaaaatt tgcactagtg aaggaatcta
    55021 ttttattaga aaaatgcaat ctccctaaag aaaaaaccac taaacagtgc tttctttcaa
    55081 cttctcctta attttttgtg ctttccctaa agtaaagttt ctggccaact ctgaatcaaa
    55141 atatagtgaa cgttttttaa aatctatttc tttaacgtta aagatattta ctaaacacgc
    55201 tcgactaatt cttgttaaca taggatattt cttttcaagc tcactaattc ttccataaaa
    55261 ttcgtactgc ccatctttag tacacaatga taatctatgt ggtaattttg aagcttctac
    55321 aaaataaatg tcatttatat caaaagtaaa tgtttgagaa cctatggaaa aaacaaatgc
    55381 ttgattcgta acctgttttg ttgcatctat cctttcttgc gctaataaca ataactcaac
    55441 aatctcgttt cgatactcat ctagtgtttg atcttttgtt acaaatccta acgtttcaac
    55501 tcttcttttt attgttacgg gtaacatctc atcatgagta gttgtaaaaa tgatttttgc
    55561 ttgaacatca tattttctga ttacttctgc taattctata ccgttaactt catgatttaa
    55621 atcaatatct aagaaatata ttccttgctt tggtcggaat tgtttgaggt attttttaac
    55681 ttctaacgga ctttgtgttt tcaatactat cttaaatact ttatcgtgaa aaagaatgaa
    55741 attatcaata attcgttcta actgatttaa ttgtataatt tgatcttcac ataatattat
    55801 tggataactc ataaatcctc cttccctcaa aacaacaaaa taacttgagt agtaaattga
    55861 tacggatcgt tttgatagtt cacaaacata ttgggattct ttttattgat ttcctctata
    55921 ctagataaac ctaacccttg atgtcccttt tttgttgtta ttcctttttt cattagagta
    55981 tctatagaga gtagtatctc tctacaacta ttcgatatcg aaatttctat ctgtttatca
    56041 tctcggtaaa tagctagaga cagttctcta tcttgactct ctatagatgc ttcaattgca
    56101 ttatcgagaa taattccaag caccctaata cagtcaaata ttggaacagg aacttcaata
    56161 atagtacttg gacattcgaa tttacaacaa atcttattgt tatttgcttg gtatagcttc
    56221 gctatcaaca aacttttcaa atattcattt tttatatttt ttaaataacg atattcaaac
    56281 ttattggtta gataagaatt agaatagatc tctaattctt ttatttcttt ttccaatatc
    56341 gaatcatggt tttgattagc gatttctttt aaactcagca ataaattttt atagtcatgt
    56401 ctaaatctgg caaatttttc ttgatctttt tccagtgaat cagtatattt ttttagataa
    56461 accaattctt ttcttttcaa ttgctgttca tattgttctc gttgcctaat agttattcta
    56521 ataaataaaa atagaacaac aaacatttga atgattaaaa atattaagac accaaacaca
    56581 aaatgatcaa atacttgata ttgatgagca acataagaaa caaataaacc aattgtaagt
    56641 aaataaatca tgaatatagc agtaagttta gaactggact gttcaacaat cctatttata
    56701 gctaattttc tatataacca aataaacatc agcaaaaaca aagaatcaag cattatctgg
    56761 aatactacat aaccataccc ttttatccct tcaaatgaaa acatatacgt cataaccatg
    56821 gaagtaataa tagatctgaa atcatcaata agcatacata atagtaattt atttaaaagc
    56881 actatattag ttttcttctt ttcctttaat ataaaataac ttccgattat aggtatagct
    56941 gcggaaacat cagtaaataa ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa
    57001 agatattttc ttttgttaag atcagtatta gtcatataaa ataagagaaa aaagaaaaag
    57061 tttcccacga acattcttaa tagataagct tttgtatcga tagtaatcaa gagatcccct
    57121 cctgatttta ggatatcata aaaaaaattt tagttacact ttttaaataa tgaaaaaaca
    57181 cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc tcctttaatt
    57241 tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc ttccattacg
    57301 attcccccta caaactataa atacttttat gtcactgatt caatatacct atttttctaa
    57361 tcaaaaaatt ttg
//
LOCUS       DADQRX010000007        54371 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.007, whole genome shotgun sequence.
ACCESSION   DADQRX010000007 DADQRX010000000
VERSION     DADQRX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.007"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..328)
                     /locus_tag="J8V14_000407"
     CDS             complement(26..328)
                     /locus_tag="J8V14_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0920541.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="J8V14_000408"
     CDS             complement(353..2446)
                     /locus_tag="J8V14_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBA0920542.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="J8V14_000409"
     CDS             complement(2935..3699)
                     /locus_tag="J8V14_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBA0920543.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="J8V14_000410"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="J8V14_000410"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBA0920544.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="J8V14_000411"
     CDS             complement(4704..5780)
                     /locus_tag="J8V14_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA0920545.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="J8V14_000412"
     CDS             complement(5804..6397)
                     /locus_tag="J8V14_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBA0920546.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="J8V14_000413"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="J8V14_000413"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBA0920547.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="J8V14_000414"
     CDS             complement(8067..8366)
                     /locus_tag="J8V14_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBA0920548.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="J8V14_000415"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="J8V14_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBA0920549.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="J8V14_000416"
     CDS             complement(10121..11032)
                     /locus_tag="J8V14_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBA0920550.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="J8V14_000417"
     CDS             11152..12009
                     /locus_tag="J8V14_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0920551.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="J8V14_000418"
     CDS             complement(12196..12558)
                     /locus_tag="J8V14_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920552.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="J8V14_000419"
     CDS             12904..14343
                     /locus_tag="J8V14_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBA0920553.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="J8V14_000420"
     CDS             complement(14350..16902)
                     /locus_tag="J8V14_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBA0920554.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="J8V14_000421"
     CDS             complement(17016..18347)
                     /locus_tag="J8V14_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0920555.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="J8V14_000422"
     CDS             complement(18313..19791)
                     /locus_tag="J8V14_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0920556.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="J8V14_000423"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="J8V14_000423"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBA0920557.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="J8V14_000424"
     CDS             22401..23828
                     /locus_tag="J8V14_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA0920558.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="J8V14_000425"
     CDS             complement(23920..24285)
                     /locus_tag="J8V14_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBA0920559.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="J8V14_000426"
     CDS             complement(24282..24647)
                     /locus_tag="J8V14_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBA0920560.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="J8V14_000427"
     CDS             complement(24839..25087)
                     /locus_tag="J8V14_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920561.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="J8V14_000428"
     CDS             25086..25214
                     /locus_tag="J8V14_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBA0920562.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="J8V14_000429"
     CDS             25271..26965
                     /locus_tag="J8V14_000429"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBA0920563.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27032..27238
                     /locus_tag="J8V14_000430"
     CDS             27032..27238
                     /locus_tag="J8V14_000430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920564.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="J8V14_000431"
     CDS             27616..28749
                     /locus_tag="J8V14_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBA0920565.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="J8V14_000432"
     CDS             28780..29100
                     /locus_tag="J8V14_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920566.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="J8V14_000433"
     CDS             complement(29129..29593)
                     /locus_tag="J8V14_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920567.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..30978)
                     /locus_tag="J8V14_000434"
     CDS             complement(29725..30978)
                     /locus_tag="J8V14_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0920568.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31007..31324)
                     /locus_tag="J8V14_000435"
     CDS             complement(31007..31324)
                     /locus_tag="J8V14_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBA0920569.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31350..31670)
                     /locus_tag="J8V14_000436"
     CDS             complement(31350..31670)
                     /locus_tag="J8V14_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0920570.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32134..33549)
                     /locus_tag="J8V14_000437"
     CDS             complement(32134..33549)
                     /locus_tag="J8V14_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0920571.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33566..34882)
                     /locus_tag="J8V14_000438"
     CDS             complement(33566..34882)
                     /locus_tag="J8V14_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0920572.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35032..35730)
                     /locus_tag="J8V14_000439"
     CDS             complement(35032..35730)
                     /locus_tag="J8V14_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBA0920573.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36019..37008)
                     /locus_tag="J8V14_000440"
     CDS             complement(36019..37008)
                     /locus_tag="J8V14_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBA0920574.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="J8V14_000441"
     CDS             complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="J8V14_000441"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA0920575.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38096..39730)
                     /locus_tag="J8V14_000442"
     CDS             complement(38096..39730)
                     /locus_tag="J8V14_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920576.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39717..41564)
                     /locus_tag="J8V14_000443"
     CDS             complement(39717..41564)
                     /locus_tag="J8V14_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920577.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            41551..43188
                     /locus_tag="J8V14_000444"
     CDS             41551..43188
                     /locus_tag="J8V14_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBA0920578.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(43333..44073)
                     /gene="truA"
                     /locus_tag="J8V14_000445"
     CDS             complement(43333..44073)
                     /gene="truA"
                     /locus_tag="J8V14_000445"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBA0920579.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(44264..44899)
                     /locus_tag="J8V14_000446"
     CDS             complement(44264..44899)
                     /locus_tag="J8V14_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBA0920580.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(45046..46287)
                     /gene="sstT"
                     /locus_tag="J8V14_000447"
     CDS             complement(45046..46287)
                     /gene="sstT"
                     /locus_tag="J8V14_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBA0920581.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            46485..47483
                     /locus_tag="J8V14_000448"
     CDS             46485..47483
                     /locus_tag="J8V14_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920582.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            47496..49514
                     /locus_tag="J8V14_000449"
     CDS             47496..49514
                     /locus_tag="J8V14_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA0920583.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(49574..50290)
                     /locus_tag="J8V14_000450"
     CDS             complement(49574..50290)
                     /locus_tag="J8V14_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBA0920584.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(50517..51059)
                     /locus_tag="J8V14_000451"
     CDS             complement(50517..51059)
                     /locus_tag="J8V14_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA0920585.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(51064..51291)
                     /locus_tag="J8V14_000452"
     CDS             complement(51064..51291)
                     /locus_tag="J8V14_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HBA0920586.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(51370..53250)
                     /locus_tag="J8V14_000453"
     CDS             complement(51370..53250)
                     /locus_tag="J8V14_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBA0920587.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(53439..54053)
                     /locus_tag="J8V14_000454"
     CDS             complement(53439..54053)
                     /locus_tag="J8V14_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920588.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            54195..>54371
                     /locus_tag="J8V14_000455"
     CDS             54195..>54371
                     /locus_tag="J8V14_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0920589.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT        16640 a        11007 c         9028 g        17696 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
    30001 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
    30061 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
    30121 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
    30181 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
    30241 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
    30301 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
    30361 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
    30421 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
    30481 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
    30541 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
    30601 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
    30661 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
    30721 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
    30781 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
    30841 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
    30901 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
    30961 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
    31021 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
    31081 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
    31141 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
    31201 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
    31261 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
    31321 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
    31381 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
    31441 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
    31501 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
    31561 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
    31621 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
    31681 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
    31741 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
    31801 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
    31861 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
    31921 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
    31981 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
    32041 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
    32101 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
    32161 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
    32221 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
    32281 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
    32341 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    32401 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    32461 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    32521 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    32581 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    32641 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    32701 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    32761 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    32821 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    32881 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    32941 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    33001 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    33061 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    33121 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    33181 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    33241 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    33301 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    33361 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    33421 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    33481 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    33541 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    33601 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    33661 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    33721 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    33781 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    33841 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    33901 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    33961 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    34021 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    34081 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    34141 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    34201 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    34261 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    34321 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    34381 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    34441 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    34501 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    34561 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    34621 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    34681 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    34741 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    34801 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    34861 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    34921 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    34981 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    35041 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    35101 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    35161 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    35221 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    35281 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    35341 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    35401 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    35461 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    35521 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    35581 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    35641 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    35701 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    35761 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    35821 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    35881 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    35941 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    36001 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    36061 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    36121 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    36181 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    36241 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    36301 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    36361 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    36421 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    36481 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    36541 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    36601 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    36661 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    36721 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    36781 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    36841 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    36901 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    36961 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    37021 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    37081 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    37141 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    37201 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    37261 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    37321 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    37381 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    37441 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    37501 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    37561 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    37621 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    37681 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    37741 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    37801 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    37861 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    37921 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    37981 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    38041 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    38101 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    38161 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    38221 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    38281 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    38341 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    38401 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    38461 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    38521 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    38581 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    38641 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    38701 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    38761 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    38821 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    38881 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    38941 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    39001 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    39061 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    39121 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    39181 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    39241 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    39301 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    39361 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    39421 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    39481 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    39541 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    39601 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
    39661 agatgaaaga atatatacta ctaactataa ttaatccact caatattact acttttttat
    39721 ttattttcat tcttcttgtt tatctccttt ttagtcacat atcgagaatc tttcaactta
    39781 ttaggtgcaa atcgtttaaa acttgaatac accactaaac aataagtcag aatactgatc
    39841 cataaagcca tgagagcagt tggagtatat tttgttgtaa tttctatctt attttctcca
    39901 gcatttacat ccacattcac taagccatgt tttttataag agtcatttac gactttaccg
    39961 tttactttta cctgtgtacc taaataagca tatactggta ctgtcacttt ttgctttttg
    40021 ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt tatacttccc tttttcccac
    40081 gattctccta cttttacttc atgtttttct aagtgaggcc tttcttttat cgcttctgtt
    40141 ggggcgtaat cgtataatcc tcccatatct gatgttgtat aacgtgggag tgtttcttca
    40201 ttgataacaa tatgaccgtg ttttgattgg ataacttttg cgtaattttc ttttgctgct
    40261 atagacgtga cgagtagaaa caccacagta gcgccaatga ttaaccatga atatttttta
    40321 ttcatagtct ttttcataaa aacaagcgtt atgatcattg aagaaaaaaa gatttggata
    40381 cctaagatac gccaaggaaa ctggagtaag cttatcggtg ttttttgaaa caaaaaccat
    40441 ggaaataaat tagttacgca tacccatgta gaaactacgc caacatagat gattttttct
    40501 tttttctcca tttctttcag tatgaataaa ggaaaaatca acgcaaccaa tcctaataac
    40561 cccatagtgt atgttgtaat atcgttattt aacgcttgaa tgatactatc tgaaatagaa
    40621 agtgcttcta gctgcaaatc tgttttgaag ggactgtgta tttcttgata attcatctgc
    40681 tgcaacatag gataccaaaa atagctagtt aatgcacaag tcaaaaaaac agcctttact
    40741 aaattcacga ccacaaattt tatatcgttt tttatttgct tgcttaaaaa caaacgaact
    40801 ataatcatac aagcaatcaa tgcactacac aatagtgtag ttaagacatg agtatacaca
    40861 agtagactta tactgacaga taaagcgatg actttttttt ctctattgaa tatcaatcca
    40921 taagcataat ataaaacagc tgggataatc gtaagagcga ttgcttctgc aattgcggat
    40981 cgataataga tatcaactgt tcgataagtt gcaaaagtat ataaacaact aaataaaaaa
    41041 gcagcatcta tctttttaaa aaacttttct ccataataaa aactaatcag aatcgtacaa
    41101 atatttaata accaaatata tagaacataa cttaatatta agttatttga aattccataa
    41161 aaaacgactg caggaattat tgtaagaaaa ggataaaaat agttcagtcc atctccataa
    41221 ttgttaaatg ttgtatagtt gattggccct tcaaaaatag aagataaccc tttgattcgt
    41281 gaaagatgaa aagataaatc acctgttgta taaatttctt gagcatttct cataaaattt
    41341 actgaatata aagtactaag taagataaga actactaaaa gaaatgcttt tttacttatt
    41401 ttcatagatt tgttgttctc cttttttaat tctattttca gtatactaga attgtctttt
    41461 tttaataagt tttgcgttaa atttttcata gcactagtat gatatactag aaaagaatca
    41521 atttttatta cttttaaata ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat
    41581 atcattttta tgttatttct aagttttgct tacgtcatcc ctctattcat gtctaaggga
    41641 atctatcata gtgtcaatca ggatacttac tttcatttat ctcgaatcat aggactagat
    41701 aatgtatgga gttctccagt aaacttcaat aacttcgatc atcatggaac aatgatgaat
    41761 attttttatc catggcttac tctttatcca gcttttttat tttataaaat gatgggaaat
    41821 cttgttttaa gttataatat ctactatttt tttatcactt ttttaacaat ggtcgtttct
    41881 tatttttcta tgaaacaaat aaaaaacaat cgttatattt cgctgttatt ttcgattatt
    41941 tatacatttt ccgcctatcg tgccattgac atattccatc gtgcttcatt gggagaagct
    42001 gtcgctttaa catttttgcc tttgatacta atggggtgtt acgaaattta tattagagat
    42061 taccaaaaat ggtactggtt gtctattggc atgacgctag tagtgtatac tcatttactt
    42121 tctgtcgcta tggtttctgt atttattgga ggaacgttgt ttctttcttt ttatttttgg
    42181 gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta ccgtcctcac ctttttttta
    42241 agtgcgggat tcttaattcc ttttattcaa caatctagag cacaagaatt gaaagtccca
    42301 ctgggaaaag aattaagtgg tatggctcct agtgatatgc tgacacatat tttaaataat
    42361 aactataaca actatactat tggattattc ttatttctcg gattgattgg tgcttttatt
    42421 tttataaaaa aattaactgc tgatgattta tttattttct ttcttggtgt atttgttctt
    42481 ttttgttcaa ccaatttatt tccttggcaa ctttttaacc atacaccagt taagagtcta
    42541 caatttgtct ggcggttaaa tggtttttct tctttattta tagcctatac gatgagtata
    42601 gtgatctatt atattttttc tactaaaaat aattcatgga aaataatggt ctttacgttt
    42661 cttgcactaa tattgcatct ctcaggagta agcaattcta tccatgctaa caaagattca
    42721 ttaacactta tagcacctga agacgctgtt gctatagctg aaaattacaa tcatactgat
    42781 tatgcaaaca aagagtcaat ctatcatcct gatatgatta ataatgatca atatttacta
    42841 ggtaaccagg aaataaatcc aacaactcat tttatgagta acaaacttac aattgaatta
    42901 gataattcga ataataaaaa tactgtcttg acgacaccta tttatcgtta taaagggcaa
    42961 gtggcttcta ttaatggcaa actagtacaa acaaaattat ctaagtttgg cacaactgaa
    43021 ttaactattc ctccaggtat aaataaagtc gttattactt atcagtacac aaaactagca
    43081 attgcttcaa gatatttatc tatcgttact ttgattttat ttttacttta tcgatttact
    43141 ttttctaaac aaaaacaacc gagaagagaa gtacaacatt ctcactagat ttttatttaa
    43201 gaaagacttt ttagctgtac acataaacag gctaaaaagt ctttttttat atttctttaa
    43261 tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt
    43321 atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt
    43381 ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat
    43441 ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa
    43501 tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc
    43561 aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg
    43621 tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa
    43681 ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct
    43741 gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct
    43801 tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc
    43861 aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt
    43921 tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat
    43981 ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc
    44041 gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt
    44101 tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag
    44161 acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac
    44221 cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca
    44281 agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac
    44341 ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg
    44401 ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc
    44461 cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta
    44521 aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga
    44581 tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa
    44641 ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca
    44701 ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg
    44761 aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc
    44821 ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa
    44881 atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta
    44941 ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac
    45001 tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa
    45061 tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc
    45121 tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg
    45181 gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc
    45241 aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc
    45301 acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat
    45361 ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat
    45421 attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct
    45481 ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg
    45541 aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa
    45601 aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag
    45661 taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac
    45721 caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc
    45781 tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc
    45841 caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg
    45901 attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt
    45961 ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc
    46021 tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt
    46081 cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg
    46141 tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt
    46201 ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa
    46261 cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg
    46321 tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca
    46381 accatttctt gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa
    46441 gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt
    46501 tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa
    46561 tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc
    46621 ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg
    46681 ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt
    46741 taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc acactagttt
    46801 gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
    46861 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
    46921 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
    46981 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
    47041 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
    47101 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
    47161 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
    47221 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
    47281 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
    47341 attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
    47401 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
    47461 atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat
    47521 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
    47581 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
    47641 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
    47701 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
    47761 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
    47821 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
    47881 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc
    47941 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
    48001 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
    48061 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
    48121 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
    48181 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
    48241 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
    48301 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
    48361 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
    48421 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
    48481 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
    48541 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
    48601 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
    48661 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
    48721 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
    48781 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
    48841 tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
    48901 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
    48961 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
    49021 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
    49081 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
    49141 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
    49201 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
    49261 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
    49321 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
    49381 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
    49441 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
    49501 aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc
    49561 cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt
    49621 cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg
    49681 tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat
    49741 atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc
    49801 cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt
    49861 tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac
    49921 ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg
    49981 atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg
    50041 aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact
    50101 ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc
    50161 tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg
    50221 attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc
    50281 tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc
    50341 aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg
    50401 agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt
    50461 cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat agacttttag
    50521 tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact
    50581 tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat
    50641 ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa
    50701 tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg
    50761 taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc
    50821 actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa
    50881 agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc
    50941 atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta
    51001 tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt
    51061 gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc
    51121 agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt
    51181 aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg
    51241 catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct
    51301 tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct
    51361 tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc
    51421 gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat
    51481 aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt
    51541 ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa
    51601 attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat
    51661 agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa
    51721 agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa
    51781 taattcagca tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg
    51841 attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg
    51901 ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc
    51961 gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg
    52021 ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt
    52081 atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc
    52141 taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg
    52201 atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt
    52261 atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa
    52321 gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc
    52381 ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc
    52441 gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc
    52501 aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa
    52561 tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa
    52621 ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt
    52681 tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc
    52741 ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc
    52801 agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt
    52861 tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga
    52921 ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt
    52981 aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac
    53041 gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg
    53101 tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt
    53161 aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg
    53221 atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg
    53281 agactaagta tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa
    53341 aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa
    53401 agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac
    53461 agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt
    53521 agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg
    53581 aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct
    53641 tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc
    53701 tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta
    53761 gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga
    53821 tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca
    53881 tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa
    53941 agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa
    54001 tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg
    54061 cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc
    54121 tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat
    54181 aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg
    54241 ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac
    54301 taacagaaac ggcagatgta catcgttcga ctttttatac acattataag gatatctatg
    54361 atctatatga t
//
LOCUS       DADQRX010000008        52112 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.008, whole genome shotgun sequence.
ACCESSION   DADQRX010000008 DADQRX010000000
VERSION     DADQRX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 52112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.008"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..357
                     /locus_tag="J8V14_000456"
     CDS             <1..357
                     /locus_tag="J8V14_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBA0920590.1"
                     /translation="SKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRA
                     EIIAKSGDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLI
                     LLFLWVHKKKKKGETT"
     gene            371..706
                     /locus_tag="J8V14_000457"
     CDS             371..706
                     /locus_tag="J8V14_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920591.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            690..3170
                     /locus_tag="J8V14_000458"
     CDS             690..3170
                     /locus_tag="J8V14_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920592.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            3226..4785
                     /locus_tag="J8V14_000459"
     CDS             3226..4785
                     /locus_tag="J8V14_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0920593.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            4782..6146
                     /locus_tag="J8V14_000460"
     CDS             4782..6146
                     /locus_tag="J8V14_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA0920594.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            6317..7387
                     /locus_tag="J8V14_000461"
     CDS             6317..7387
                     /locus_tag="J8V14_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920595.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            7402..8070
                     /locus_tag="J8V14_000462"
     CDS             7402..8070
                     /locus_tag="J8V14_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920596.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            8090..8668
                     /locus_tag="J8V14_000463"
     CDS             8090..8668
                     /locus_tag="J8V14_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0920597.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            9069..9980
                     /locus_tag="J8V14_000464"
     CDS             9069..9980
                     /locus_tag="J8V14_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0920598.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10358..11275)
                     /locus_tag="J8V14_000465"
     CDS             complement(10358..11275)
                     /locus_tag="J8V14_000465"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBA0920599.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            11477..11914
                     /gene="zapA"
                     /locus_tag="J8V14_000466"
     CDS             11477..11914
                     /gene="zapA"
                     /locus_tag="J8V14_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBA0920600.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            11972..12520
                     /locus_tag="J8V14_000467"
     CDS             11972..12520
                     /locus_tag="J8V14_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBA0920601.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            12635..14995
                     /locus_tag="J8V14_000468"
     CDS             12635..14995
                     /locus_tag="J8V14_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBA0920602.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            15082..15396
                     /gene="trxA"
                     /locus_tag="J8V14_000469"
     CDS             15082..15396
                     /gene="trxA"
                     /locus_tag="J8V14_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBA0920603.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            15578..17356
                     /gene="uvrC"
                     /locus_tag="J8V14_000470"
     CDS             15578..17356
                     /gene="uvrC"
                     /locus_tag="J8V14_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBA0920604.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            17501..18400
                     /locus_tag="J8V14_000471"
     CDS             17501..18400
                     /locus_tag="J8V14_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920605.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            18393..19655
                     /locus_tag="J8V14_000472"
     CDS             18393..19655
                     /locus_tag="J8V14_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920606.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            19816..21714
                     /locus_tag="J8V14_000473"
     CDS             19816..21714
                     /locus_tag="J8V14_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBA0920607.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(22044..22763)
                     /locus_tag="J8V14_000474"
     CDS             complement(22044..22763)
                     /locus_tag="J8V14_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBA0920608.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            23100..23432
                     /locus_tag="J8V14_000475"
     CDS             23100..23432
                     /locus_tag="J8V14_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920609.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            23419..25422
                     /locus_tag="J8V14_000476"
     CDS             23419..25422
                     /locus_tag="J8V14_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBA0920610.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            25441..25911
                     /locus_tag="J8V14_000477"
     CDS             25441..25911
                     /locus_tag="J8V14_000477"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBA0920611.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            25932..26516
                     /locus_tag="J8V14_000478"
     CDS             25932..26516
                     /locus_tag="J8V14_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBA0920612.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            26531..27517
                     /locus_tag="J8V14_000479"
     CDS             26531..27517
                     /locus_tag="J8V14_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBA0920613.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            27507..27818
                     /locus_tag="J8V14_000480"
     CDS             27507..27818
                     /locus_tag="J8V14_000480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBA0920614.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            27840..29621
                     /locus_tag="J8V14_000481"
     CDS             27840..29621
                     /locus_tag="J8V14_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBA0920615.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            29614..30990
                     /locus_tag="J8V14_000482"
     CDS             29614..30990
                     /locus_tag="J8V14_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBA0920616.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            30997..31632
                     /locus_tag="J8V14_000483"
     CDS             30997..31632
                     /locus_tag="J8V14_000483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBA0920617.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            31662..33017
                     /locus_tag="J8V14_000484"
     CDS             31662..33017
                     /locus_tag="J8V14_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBA0920618.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(33209..34603)
                     /locus_tag="J8V14_000485"
     CDS             complement(33209..34603)
                     /locus_tag="J8V14_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA0920619.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            34695..35666
                     /locus_tag="J8V14_000486"
     CDS             34695..35666
                     /locus_tag="J8V14_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBA0920620.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            35663..36445
                     /locus_tag="J8V14_000487"
     CDS             35663..36445
                     /locus_tag="J8V14_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBA0920621.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(36614..36985)
                     /locus_tag="J8V14_000488"
     CDS             complement(36614..36985)
                     /locus_tag="J8V14_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBA0920622.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            37119..37562
                     /locus_tag="J8V14_000489"
     CDS             37119..37562
                     /locus_tag="J8V14_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBA0920623.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(37605..38414)
                     /gene="yidA"
                     /locus_tag="J8V14_000490"
     CDS             complement(37605..38414)
                     /gene="yidA"
                     /locus_tag="J8V14_000490"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBA0920624.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(38433..39800)
                     /locus_tag="J8V14_000491"
     CDS             complement(38433..39800)
                     /locus_tag="J8V14_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBA0920625.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(39784..40614)
                     /locus_tag="J8V14_000492"
     CDS             complement(39784..40614)
                     /locus_tag="J8V14_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBA0920626.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            40777..41214
                     /locus_tag="J8V14_000493"
     CDS             40777..41214
                     /locus_tag="J8V14_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBA0920627.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            41290..41910
                     /locus_tag="J8V14_000494"
     CDS             41290..41910
                     /locus_tag="J8V14_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBA0920628.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            42099..42605
                     /locus_tag="J8V14_000495"
     CDS             42099..42605
                     /locus_tag="J8V14_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBA0920629.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            42622..43521
                     /locus_tag="J8V14_000496"
     CDS             42622..43521
                     /locus_tag="J8V14_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBA0920630.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            43608..44492
                     /locus_tag="J8V14_000497"
     CDS             43608..44492
                     /locus_tag="J8V14_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBA0920631.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(44684..44755)
                     /locus_tag="J8V14_000498"
     tRNA            complement(44684..44755)
                     /locus_tag="J8V14_000498"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44720..44722),aa:Arg,seq:ccg)
     gene            44815..46149
                     /gene="rpoN"
                     /locus_tag="J8V14_000499"
     CDS             44815..46149
                     /gene="rpoN"
                     /locus_tag="J8V14_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBA0920632.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            46352..47389
                     /locus_tag="J8V14_000500"
     CDS             46352..47389
                     /locus_tag="J8V14_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HBA0920633.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            47430..48431
                     /gene="gap"
                     /locus_tag="J8V14_000501"
     CDS             47430..48431
                     /gene="gap"
                     /locus_tag="J8V14_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA0920634.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            48541..49731
                     /locus_tag="J8V14_000502"
     CDS             48541..49731
                     /locus_tag="J8V14_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBA0920635.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            49835..50590
                     /locus_tag="J8V14_000503"
     CDS             49835..50590
                     /locus_tag="J8V14_000503"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBA0920636.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            50706..52004
                     /gene="eno"
                     /locus_tag="J8V14_000504"
     CDS             50706..52004
                     /gene="eno"
                     /locus_tag="J8V14_000504"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBA0920637.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17290 a         9056 c        11012 g        14754 t
ORIGIN      
        1 agcaagatct tttatgaaaa tcagctttat attgaaaata cgtctaatgc atttttagtt
       61 gcaccaaatt cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc
      121 gattaccgcg cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa
      181 aactttacga tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta
      241 caagaacaat ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg
      301 ttgattttgc tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga
      361 ggtgatttcg ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt
      421 cagctggcaa gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga
      481 tctaagtcca attccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca
      541 gctaccgatt tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaacaaaa
      601 gatccgtttt ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt
      661 atggagaaga aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc
      721 gctccttctt ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag
      781 aagctgttgg agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg
      841 tacctccaaa tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg
      901 ctaaccaatt gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga
      961 aaggtggcac ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt
     1021 tggtgaatat cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg
     1081 tatcaagcag tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg
     1141 gtgcccccat caaggttact agcggaagtt tattatcagc aggaagccat accttgcgtt
     1201 ttgggatggg gtcgtgggat acaaatactc tattgacaga tattattgct attattacgc
     1261 gaggcgaatt caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga
     1321 agatgacaga aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta
     1381 aattacaacc ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata
     1441 atattatcca agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc
     1501 ctaaattaac tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga
     1561 taaatacgtg gaatagttac atctcaccaa gtgattcaac aggaaaatat aatacatttg
     1621 ctgataccac agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc
     1681 ggacattgac gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg
     1741 atcgcgtagt caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata
     1801 ctccagcaag cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg
     1861 cgcaaggcgg attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg
     1921 gaacggtttt accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg
     1981 catcgtacca agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt
     2041 acaggcttta ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc
     2101 aaacgatcca atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg
     2161 cttcagggca atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc
     2221 cgtcttttgc gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa
     2281 cagtggctaa ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac
     2341 ctgagacagg agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat
     2401 tcacgttaac aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg
     2461 aaaatactac ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg
     2521 gcattcaaca aggagtttct gtcccagcag atctattgag caaaatagga gataccattc
     2581 atcttactta caactatcaa ttaaatactg tagatacttc tttacaatcc gtcagcatac
     2641 tgacaaaggc agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta
     2701 acccagttgt ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag
     2761 ttaatgtacc agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt
     2821 attatgaatc aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt
     2881 ctccttggca actgacaggt acattgactt ctttatttaa gaatgatcaa ggtcaagaat
     2941 taagcggaac aaaattatac ttcaatcatt ctggatcaaa gcaattgatt cagcaaggtc
     3001 aaaacacgtt gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc
     3061 ccgataccga tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa
     3121 cgtatcaagg catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa
     3181 tttgtatgga tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt
     3241 ctgctgttaa aatcagaaat gcttgaagga gtggacttgg aacttttcag tttgcttgaa
     3301 aaaaatgaac aacggcaaat caaacttttc caacttttat tagaggaaaa aggagaggtt
     3361 tccgttaata acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg
     3421 ttttatttac aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg
     3481 aatattttat taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgttac
     3541 ttgcgctatt ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat
     3601 cggcagaaac ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag
     3661 gaattaaatg attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt
     3721 ccagaaagtc aaattagtta tttttatttc caattatttt ggtttggacg tccctatctt
     3781 gaaaacaaaa aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa
     3841 gagtttagtt ttgctattac agaagaagga cgattgaaat ttgaaatatg gctttccatt
     3901 atgaagaagc gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt
     3961 ggattgtttc aaacgacaca atttttattg gacctccata catttttgac ccgttacttc
     4021 tttcaatatg cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc
     4081 agtaacttta cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa
     4141 tacaaagacg acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat
     4201 ttctcttttt atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa
     4261 aatcacgctc attttgccta ttttagagga tggatgaata tttttggcca agaaggatta
     4321 aaacagcggt taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc
     4381 agacaaacat ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa
     4441 ttatttgctc gctatgcatc tattttacgg acgctgatca agcagttaga attacgtctg
     4501 caaattgctt atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc
     4561 caggaacaat tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag
     4621 tatgatatca ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt
     4681 tttgtattca ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa
     4741 gaatgttatt tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg
     4801 gtgcttccca tgaaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag
     4861 aagttttact gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca
     4921 acctttattt aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc
     4981 ttcaagaaag tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca
     5041 aacatcagct catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat
     5101 taatcgtagc gacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg
     5161 tctacaccta taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa
     5221 aagaagtagt gatcttcggt gctggttaca ttggattgga attagcagat gctttgagaa
     5281 ataaaggata cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg
     5341 ataaggatat gatcaacagt tttcaaaatc agctgcaaac taagcaaata aatttttatc
     5401 caaatgaatt cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc
     5461 tgtctcaaac aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga
     5521 cattgttaaa agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc
     5581 tgcagacaag tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca
     5641 aaaataaacg gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag
     5701 tagcgctaac tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag
     5761 cagtcattat agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct
     5821 tggaacaaaa taccggttcg tgttcaggag aatttgatct atttccacaa tacgacgaag
     5881 aaaacaaaac agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg
     5941 gacagctgat aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga
     6001 aacacaaaac tacgattcca cagctagcag ttgaggattt tggatttctg tctgaataca
     6061 cctctcgttt tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata
     6121 gcaatagagt agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata
     6181 ataaatatta atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt
     6241 gagcataata ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt
     6301 caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta
     6361 tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt
     6421 agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat
     6481 tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc
     6541 agaagaagtc gaaggtggga aagtggcgta tcttgcccaa acacctggag tcgtgactag
     6601 taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca
     6661 attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg
     6721 cgatattagt ttgaaagaag aatatggtct agccatagga gatacagtta agttatctgg
     6781 tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc
     6841 agttctctat acaaccatca atgcttacca agaaatccgc ttcgaaaaag aagatacaag
     6901 tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga
     6961 tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt
     7021 attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat
     7081 tttcatttat gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg
     7141 gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt
     7201 tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt
     7261 tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgt tagtagtagc
     7321 tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat
     7381 tggataagaa ggaggaagaa catgaaagca atagaattta aagaagtaga aaaaagtttc
     7441 caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg
     7501 gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca
     7561 ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat
     7621 caaaaagaaa gcgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct
     7681 tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga taagattaaa
     7741 catgaacatc gtcaaaaaga agcaaacgaa ttatttgagc agcttggcgt ggataagttg
     7801 atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt
     7861 gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag tttggattca
     7921 gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca
     7981 acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg
     8041 gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg
     8101 atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa
     8161 gggatatctc ggcacttcaa caagagaaat cgcacaaaaa gttgggatca cccagcccaa
     8221 tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt
     8281 aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt
     8341 aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat
     8401 gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg
     8461 gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag
     8521 aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat
     8581 cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata
     8641 tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaat agttaggtga
     8701 agaagcaaga tatgacgaag atcgttatat cttgcttctt tatataggta gtttttcata
     8761 aatacaaatt tgttattctc tcatgattat cagggtatat ttttatttat tatgtcggtg
     8821 ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa tgaaggaaac
     8881 tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg atatgccaaa
     8941 aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt ttacagcccc
     9001 ttttttgctt acttaagctg attttaagtt ttggccctta gtctggatct acgaggtgag
     9061 agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc attattatct
     9121 gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc agcagtacga
     9181 acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag acattaatca
     9241 aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc ccacttgcaa
     9301 ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct tatcagatca
     9361 agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga
     9421 atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat cctgggcatg
     9481 gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa gatataacat
     9541 taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg gtttatttga
     9601 cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt gaagaaaaaa
     9661 aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat gaagcaactg
     9721 gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca attgctgact
     9781 ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat caagttcttc
     9841 gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat gatagtgatt
     9901 taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg acacaagcat
     9961 tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc ctcatatttt
    10021 tcgtttggcc gggcttatta tcgcagatgc aagcttttaa acaccgttta aggaagaggt
    10081 cgtgagcctg ccgttcaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
    10141 tttgatgact attgtgactt gtgaccgagg agttggcatg agaacaccgt ttaagaaaaa
    10201 ggttgtgaaa tcaacgtttt gttctgagta ttagagaaac aaacggaaaa atagttcctc
    10261 atattttgcc gtttgttgag ctaataccga ggatcagatg attgaacacc gtttattcgg
    10321 aacaagggac tgtgacatga cttttgtcac agttccatta tttttttagt agcttcaaag
    10381 ctttttctgt attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt
    10441 tctccaatag ttttgccgct acttggtcag atttcgttcc agctcctgac ggaagggtga
    10501 ttccagcttc agctgatgct ttgtctaact cttccaaaaa gctggcacga ctgatgatag
    10561 aagcagcagc aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt
    10621 gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat
    10681 cgatcaaaat cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt
    10741 gattgtgcaa agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt
    10801 tttctggaga taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct
    10861 ggatgatttg gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg
    10921 caagctgatc acgtttcaca taagcagcac atactgttag cggaccgaaa tagctgccat
    10981 ttccgacctc atcactacct aaaacggata attgactgat attttttggc aaagaagagg
    11041 ctgtttttgc tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg
    11101 ctgcattcgt accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt
    11161 ctttttttgc agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt
    11221 atgctttcat ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat
    11281 tctcctcgtt tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga
    11341 ttgcgagaca tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat
    11401 ttgtggagaa cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata
    11461 ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac aaagcggtga ttgccaacca
    11521 aacatatact attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat
    11581 caatgaacag ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc
    11641 aatcttgatg gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatattgga
    11701 gctggaagaa gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa
    11761 tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg
    11821 tcaagcagat cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg
    11881 aaaaggacaa attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat
    11941 ttaaaaaagt tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt
    12001 gctaattgcg tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat
    12061 tggatatgta ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt
    12121 ggaattatac attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca
    12181 ggtatttgcc tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt
    12241 gctaattggc tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt
    12301 gccgatcttg agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta
    12361 cgttactata tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa
    12421 tcaattcggc gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa
    12481 caagatgtat gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat
    12541 atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca
    12601 gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt
    12661 ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga
    12721 agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga
    12781 aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaaattga
    12841 aaatatccgt ccccatatga aacggattga gatcggagca gacttgaatg gagtggagct
    12901 tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct
    12961 ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat
    13021 ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga
    13081 cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg
    13141 tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat
    13201 caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg
    13261 cggtgtagtc cacgatcaaa gtgcttcagg tcagacttta tttattgaac caaaacaagt
    13321 ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg
    13381 tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatac
    13441 ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa
    13501 agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc
    13561 actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc
    13621 catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct
    13681 gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg
    13741 tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc
    13801 gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag
    13861 tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc
    13921 aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta
    13981 tcctgaattg aaagtttatg gatataatcg ggcaaatacg attaatgcca gcatggagtt
    14041 tgatgtggat acattaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa
    14101 tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca
    14161 gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg
    14221 gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact
    14281 tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga
    14341 aaaagcaaag aaaaaagcaa atgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat
    14401 cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca
    14461 gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa
    14521 taaagtattg aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt
    14581 tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga
    14641 aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa
    14701 agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa
    14761 tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt
    14821 ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg
    14881 cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga
    14941 gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt
    15001 actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta gtcatagatt
    15061 ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa
    15121 acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg
    15181 caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct
    15241 aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca
    15301 acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa
    15361 gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga
    15421 gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa
    15481 attaatattt tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc
    15541 tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa
    15601 attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat
    15661 catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggtag
    15721 ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac
    15781 agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa
    15841 atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa
    15901 atatccccgt ttggtgatta ctcgtaaggt actaaaagac aaagcactgt attttggacc
    15961 atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct
    16021 gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg
    16081 cctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt
    16141 gaaaaacttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga
    16201 aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc
    16261 cattgagaca atcatgactt gccaaaaaat gacaaatact gatttactgg atcgagatgt
    16321 atttggttat gcgattgata aaggatggat gtgtgtccaa gtgtttttcg taagacaagg
    16381 aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt
    16441 cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt
    16501 gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca
    16561 accaaaacga ggagaaaaga aaaaattagt tgaattggcg agtaagaatg cctcagttgc
    16621 tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga
    16681 acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc
    16741 taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc
    16801 aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc
    16861 aatgagagaa gtcatctatc gcagatattc gcgagtgatt cgcgaagggt tgcctttacc
    16921 tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga
    16981 aaatcaatta ggaatcgata ttcctattgc tggcatggta aaaaacgata aacataaaac
    17041 aaatgagttg ctattcggta cagatttggc tgtagtaccg cttgagcgaa attcaccaga
    17101 gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg
    17161 gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc
    17221 aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac
    17281 gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa
    17341 attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta
    17401 tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg
    17461 tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct
    17521 tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg
    17581 aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat
    17641 cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga
    17701 gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac
    17761 gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga
    17821 accaaaaatc gacttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt
    17881 aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga
    17941 acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt
    18001 gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca
    18061 caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat
    18121 ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt
    18181 ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct
    18241 tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt
    18301 tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga
    18361 agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag
    18421 atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta
    18481 tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata
    18541 aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag
    18601 atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag
    18661 aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt
    18721 atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct
    18781 tgcaatcgat gcttcgagcg agtcaattag gcatttcacc agaagaagta gctagcttga
    18841 gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag
    18901 tcggagaaga taactcgaca gtccaatacg tcgtaagtta tgtagcaacg attgtcttgt
    18961 tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca
    19021 cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaat
    19081 taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta
    19141 taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat
    19201 tggataactt gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt
    19261 tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag
    19321 caaaagcaat tttgccagtg acttatctat ctctaggcgg atacatgctt ggattgatac
    19381 taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat
    19441 cttcatatat tatgccgatt cgcttagcaa atgagacagt cggtatgggt ggagcattga
    19501 tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt
    19561 acaaatcgaa tgtactcgtt tatagcgaag gcggattatg gagttcattg aaacaatcga
    19621 tttcaattat gaggaatgaa cgaaaaaaag cataaaacaa tcctaaaata cagaaggaag
    19681 ctggacatct attgttccca gcttcttttt gtatagacct attttattta ttttactata
    19741 ttggatatat attcattgaa aatagtgtat aacaacttta taatattgag tataccaatt
    19801 tttggaggcg ttttaatgga ccgttattta gatttacttt cagaaaaatt cccctcaacg
    19861 gaggatgtca tcacggaaat catcaatctc gaagcaattt tgcagcttcc aaaaggaaca
    19921 gagttatttt tgagtgatat acatggcgaa tttcctgctt ttgatcatat tctgcgtatc
    19981 ggttcaggaa atcttaaaga aaagatcaaa gacctttttt ctgaacaaat gacggaaacg
    20041 gatatcagcc aattcactat ttttacggct tatccagaat atgcattaac gactgattgg
    20101 tacaaagaac aagataaatc gcagctgatc catcatttga tcgatctttt acggtttacg
    20161 acagtcaaat atacccggtc aaaagttcga aaaggattgc cgaaagaata tagctatatc
    20221 attgaggaac tcttgtatat cgatgaccgt atcaatggaa aaaaagacta tgtaaaaaaa
    20281 atcattgaac aacttgtttt gatgaaggaa gaagaaaaat ttttaacgaa acttgcacag
    20341 acgatccaaa aatctgtcat cgatcatttg catatcgttg gagatatttt tgaccgagga
    20401 actcaagcag ccaaagtgat ggatcgaatc atggattttt cttctgtaga tatccaatgg
    20461 ggaaatcatg atattttgtg gattggtgcg tattgcggct ctgaggcatg tttactgaca
    20521 cttttacgga ttgcagcacg ctataattat ttatttgaat tagagaaaga atatgggttg
    20581 aatttacgtc cattgttttc ttttgctcat caaacttatc agaagaatcc agtatttgcg
    20641 cctaaaaaca gtaaaaatct atccgaaaga gagcaagaag aattagaaaa aatccatcag
    20701 gcactttcta tccttcaatt caaatcagaa catcagttat tggatcgtcg acctgaattt
    20761 aaaatggacg atcgtaaatt gctggaaaaa atcgactatg aagcatcaac gattgattta
    20821 gaaggtcagc tctatgagtt gaaaggaact tgttttcaaa cgatccagcc agatgatcca
    20881 aaaaaattct tggaagagga acaaaaagtg attgatagtt tgatgtattc attccagcac
    20941 tctttgagaa tgcaaaaaca tatgacgttt ttgatcgaaa aagggggaat gtatctcacg
    21001 aacaatcagc atgtgctgtt tcatggctgt atacctgtag acgagaaggg acagctctta
    21061 gcatttgaat tagaccaatc ttatcgcgga aaagaactgc tgggcttttt tgaaaaacag
    21121 ataactgaaa gcgcaaaaga tctgactgca aaagaagatt tagcaaccga tttgatttgg
    21181 tatgcgtgga gtgggccatt ttcgccttta ttcggcaaga gtaaaatggc gacttttgaa
    21241 cgttattttc tcacagagcc tcatacgcac gtcgagaaaa gcaatcctta ttatcacttg
    21301 cgagatgaag agtggttcag tgaaaaaatt ttagcagaat tcgacacaaa agaagaaggg
    21361 tcagcaatca ttaatgggca cacgccagta aaagtaaaaa aaggggagtc accgatcaaa
    21421 gcaaatggaa aagtatttgt cattgacgga ggtttatcca aagcttatca aaaaacgaca
    21481 ggaattgccg gttattcact attatgcaat tcttatggtt tccaaatcgt gacacattat
    21541 ccgtttttgc ctattgaaca acaatttgca aaaaaatccg accaaacaaa tgtgaaaaaa
    21601 gtaatcgaac agcaactacc acgcaaactc aatcgtgata caacaaaagg tatggaactg
    21661 cagcagcaga ttacccagct caaaaggctg ttggactatc gaaaggacga ttgagaagaa
    21721 ttccagtgaa cattgttcca tagactggag actgagacaa aagtcgtgtc gcagtctctt
    21781 tttccggata aacggtgttc ttctgccaac tcctcggtca caagtcacac tattcaacaa
    21841 acatgaaaag ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac
    21901 gacctcttgg tataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc
    21961 aaaaatgtga aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt
    22021 tcacaacccc ctcagaaagt ctattaaaat tcattcacaa agaaatcgaa taagcgctgt
    22081 tcagatggat gtacacggtg ggtaagttcc ggatgccatt gaacaccgag gattttatgc
    22141 atatcatctt tgctttgaac cgcttcaacg agtccatcgt tagaaaaagc gatggctttt
    22201 aataaaggtg aaagatcttt gactgcctga tgatggtaag aattcacaaa atatggatcg
    22261 gggagaaggt tgcttaacaa actgtctgaa acaatcttga cttcgtgagt cgtaaattgc
    22321 ggggcagtgg gctgttgttc atgttttact gaccactttg gatataatga aagatcttga
    22381 taaagtgtac cttccaaaac gacattcaat agttggagtc cccggcagac tgcaaaaatt
    22441 ggttttcttt gtttcaatgc ttccaagatc agtgcctgtt caaattggtc tcgttgaata
    22501 tttgtttccg ttagctttgg atgaggatct tccatgtaaa gttgtggact gacatcttga
    22561 ccacctgcaa gaataagttt gtcgatttgt ccaatcagtt gaggagctga atctggagac
    22621 atgactggca gaataagagg caatccttga gcatctaaaa ctgcatccac gaatccttgc
    22681 ggtgtatagc tcacttgatt tccttggaaa gtatctgttg ctcgaatcag ttgatttccg
    22741 gcaatgccga tgattggttt catttgtatt ccccctaaag tagacttatt gtaacaagga
    22801 tttaaaaaaa attaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta
    22861 ctatctgtaa agagaaattt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat
    22921 ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt
    22981 tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta
    23041 gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag
    23101 tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgaaaagc
    23161 gtgctcaaac actgcggcag cagctgaaga tctatgcaac agaaaaagag caggaattgg
    23221 aaacgatcca aacaaactat cgacaacaac taaaacagtt ggtggaagaa aaagaacaat
    23281 ttgaaataca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc
    23341 aagcaacttt gaaagatcaa tatcaaaagc atcagagtca agcgatcggt gcgatcattg
    23401 aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat
    23461 aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaacgt tgaaatccgg
    23521 gatctgtatc aaaatagaac aaacaaccag tgggtggaga agtattttcc agataccacg
    23581 attattgata aagaagcaaa attaagcgct ttaggtaatc ggttgaaaga catccaagaa
    23641 gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt
    23701 caagaacttt ccttacaaga tttggaagcc aattattccg aagaaaattt cataaataaa
    23761 ttgaaagaag tccttgattt aaaagaaaag tgggaacaac taactgaaga acgtgatcgt
    23821 ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg
    23881 tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt
    23941 ttcaaggaag aactgagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca
    24001 aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgttat agaagaaatt
    24061 gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa
    24121 ctgcaagaga ccaagcagca attatccatt gttcacgagc agcaaaagaa gataacgaca
    24181 gcgttaggtc agtgcagtgg ctatatccaa gaatttgaat gggtagaaga agtcacactt
    24241 gccgttgcag aacgagaaaa aataaaagaa cggtttatcc atgcgactta tctgatcgtt
    24301 ttgcaaggtt gggtagatgc agaagaaaaa cagaatttgc tgcatgtgtt aaacgaaacc
    24361 gtatcagaag aagatatata cgtcagtttc gaaacaccaa actcagaaga gattcagcag
    24421 gaagtcccta ctaaattgaa aaaccatccg cttgtcgaac cttttgaatt gctgactgaa
    24481 atgtacagtc tgccaaaata tgaagaagta gatccaacac cttggatgac accattttat
    24541 cttgtcttct tcgggatgat ggtcgcagat gttggttatg gtttgctgat gctcattggg
    24601 acaatactag ctcaaaaact acttgttcta ccacgcggga tgaaacgttt cgtaaaattc
    24661 tttgagattc tctcgatacc atcgatcatc tggggattga tctacagttc atttttcgga
    24721 cagtcattac caaaagaact gtttggcatc cgcttgcctt tcccaatcct atctacgacg
    24781 gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca gattttggtt
    24841 ggattatttg tggcagcaaa ggaaaatatc aggagaaaag attacttgag tgctgtcagt
    24901 gatggatttg cctggcaagg gatcctaatt gggatcgtta ttgcgttggt tggtgccatg
    24961 ttgataaaaa atacagcatt tgtttacctt ggcgctgggc tatcgatcat tgctgcctta
    25021 tgtatcttga tcgttccgat tatccaaacg ccgtctaaag tcaaaggtgc tgccaaaggt
    25081 gcgtataatc tttacggact gaccggctat atcggtgatt tagtcagtta tacacgtttg
    25141 atggcattgg gtatttcagg cggaagtatt ggagcggcat tcaatatgct agttgctttc
    25201 atgccgccag ctgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg
    25261 aatatgtttt tgacactgct aagtgcctat gtccatggcg cacgattgca gtatgtcgaa
    25321 ttttttggga agttttatac aggaggcgga cgtgcctttg atccgctgaa aacggcggaa
    25381 aaatacgtca acatcaatca caagaaaaaa aacaataaat aattggagga tttttatata
    25441 atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcggtatt agcgatggca
    25501 acagcgacga ttttttcagg gatcggttca gctaaaggtg ttggaatgac aggtgaggca
    25561 gcagcagcat tgacaacaag tcaacccgaa aaattcgggc aggctctgat tctgcaattg
    25621 cttccaggca cgcaaggttt gtatggattc gttattgcct ttttgatttt tatcaactta
    25681 ggaaatgaca tgtcagttgt acaaggactg aactttctag gtgcttctct gccgattgct
    25741 tttacaggct tgttttcagg tattgcccaa ggaaaagtcg cagcggcagg tattcaaatt
    25801 ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca
    25861 tacgcgattt taggtttcgt tatttccttc ctattagtgt tgaatgccta agcataaagg
    25921 aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaacgaa gcagcagagc
    25981 aggaaagagc ggcgttagaa caagaagagc gaatgaagat cgatcaaaac tttgaacaaa
    26041 aacgtacgca agttgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattactcg
    26101 aaaaaaaata ccgtcagcta aggaatcgcc agcaagtaga agtacgacaa gaaaatttga
    26161 atgcgaagca ggaattttta agacgattgt ttgcagatgc ggtaactgaa atggaaaatt
    26221 gggatgaatc tgaacaaatc cagtttatca aaaatgcatt gtattcatta cctttgacag
    26281 gaaaagtagc attcattgct ggagaaaaat cagcagccta tctttctcag acgttgttgg
    26341 atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc
    26401 aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg
    26461 tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag
    26521 ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact
    26581 tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga
    26641 aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga
    26701 acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga
    26761 gcctgaaatc gtttggatct atacgatgcg ttatactttc cataatctga aggttttgac
    26821 aaaagctgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc
    26881 attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaacttc ctgaaagcat
    26941 tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat
    27001 tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg
    27061 atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat
    27121 ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc
    27181 gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac
    27241 gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa
    27301 tattgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga
    27361 agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt
    27421 agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg aatcagaaac
    27481 aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag
    27541 attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa
    27601 ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag
    27661 agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg
    27721 ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa
    27781 aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt
    27841 tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg
    27901 atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg
    27961 agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag
    28021 gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt
    28081 cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta
    28141 atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg
    28201 aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag
    28261 aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa
    28321 caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg
    28381 gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag
    28441 aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc
    28501 cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg
    28561 ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg
    28621 agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata
    28681 caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag
    28741 cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg
    28801 gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga
    28861 tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac
    28921 ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg
    28981 aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag
    29041 tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc
    29101 aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag
    29161 tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca
    29221 tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt
    29281 ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt
    29341 tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa
    29401 tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt
    29461 atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat
    29521 atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga
    29581 tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt
    29641 gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta
    29701 atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa
    29761 gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct
    29821 gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta
    29881 ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg
    29941 gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa
    30001 acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta
    30061 ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg
    30121 gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa
    30181 gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg
    30241 tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg
    30301 actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac
    30361 atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga
    30421 agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga
    30481 agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac
    30541 gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg
    30601 actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg
    30661 cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg
    30721 aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta
    30781 ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa
    30841 aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta
    30901 gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg
    30961 ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg
    31021 acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa
    31081 ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac
    31141 aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg
    31201 gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac
    31261 gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt
    31321 gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc
    31381 gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca
    31441 gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc
    31501 aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg
    31561 aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt
    31621 accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac
    31681 aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt
    31741 tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt
    31801 ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga
    31861 atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg
    31921 gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca
    31981 gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga
    32041 tgaagctcat gctttacatc ctaaaattgg cctttgtgat ccaattgatt ggtacggttg
    32101 ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt
    32161 tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca
    32221 atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt
    32281 taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc
    32341 attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct
    32401 ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa
    32461 acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc
    32521 atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg
    32581 ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt
    32641 cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag
    32701 tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga
    32761 ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg
    32821 tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa
    32881 tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg
    32941 tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa
    33001 gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa
    33061 aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt
    33121 gaggcactac ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata
    33181 agatttgatt cctttattta tcaataggtt ataattcata tttttttatt ttttctttta
    33241 cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga
    33301 atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag
    33361 caagaaagtc attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat
    33421 agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg
    33481 ctctttttgt aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga
    33541 tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt
    33601 gttctatctt gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac
    33661 aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag
    33721 gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa
    33781 cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt
    33841 gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc
    33901 tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga
    33961 tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg
    34021 ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac
    34081 cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg
    34141 ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa
    34201 gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa
    34261 aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga
    34321 tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac
    34381 ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat
    34441 cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat
    34501 cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg
    34561 gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa
    34621 attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga
    34681 ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg
    34741 aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc
    34801 aaaatcctcg attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga
    34861 tgcctgctta tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg
    34921 ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa
    34981 acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg
    35041 tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg
    35101 gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta
    35161 cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa
    35221 aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag
    35281 cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa
    35341 gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta
    35401 catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa
    35461 gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta
    35521 cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga
    35581 ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca
    35641 aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga
    35701 tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg
    35761 tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa
    35821 aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca
    35881 cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat
    35941 taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat
    36001 ggattttcct agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat
    36061 tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga
    36121 aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat
    36181 aggagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc
    36241 aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta
    36301 ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg
    36361 ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt
    36421 ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag
    36481 agtggcaacc ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat
    36541 gaaaagtttg cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa
    36601 tgccccatac taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa
    36661 agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccagcag
    36721 ctttatgttg ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc
    36781 cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat
    36841 ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc
    36901 tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc
    36961 gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata
    37021 acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa
    37081 atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg
    37141 aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag
    37201 atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa
    37261 ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa
    37321 ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc
    37381 gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag
    37441 gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga
    37501 aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact
    37561 gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag tacatatttt
    37621 ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct
    37681 ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat
    37741 aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt
    37801 gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag
    37861 ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct
    37921 gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc
    37981 atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta
    38041 taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga
    38101 ctgccgacac ggcgtgccat tacttcgatc tctaggaaat cttcatgtgt cagtccgtga
    38161 cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg
    38221 taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga
    38281 cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt
    38341 acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact
    38401 agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta
    38461 cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag
    38521 gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct
    38581 aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa
    38641 atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg
    38701 tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa
    38761 gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt
    38821 ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat
    38881 tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag
    38941 cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct
    39001 tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa
    39061 acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata atcttccact
    39121 gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat
    39181 attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa
    39241 tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg
    39301 taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga
    39361 atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc
    39421 gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt
    39481 gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct
    39541 tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat
    39601 gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt
    39661 ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc
    39721 tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga
    39781 tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg
    39841 gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg
    39901 taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt
    39961 tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc
    40021 aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac
    40081 ttgcccacgt ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt
    40141 tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt
    40201 tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta
    40261 tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact
    40321 ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc
    40381 tgaatttctg atcactggag cgtaacctgc atcttccagt gtttggatcc cgtcacttag
    40441 aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg
    40501 taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg
    40561 ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc
    40621 ggctcctttg tataaattca tattttctac aatattatac tctaaaatca aagagttgtt
    40681 ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg
    40741 aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg
    40801 atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt
    40861 gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt
    40921 caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta
    40981 cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta
    41041 gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg
    41101 catgttagtt tgaaagaccg tcccacagca gggaaggttc gaatcgatta tgatctgttc
    41161 atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa
    41221 aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac
    41281 gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc
    41341 gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct
    41401 aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa
    41461 ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc
    41521 ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa
    41581 gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat
    41641 gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa
    41701 gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat
    41761 tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga
    41821 aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag
    41881 gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag
    41941 agacgatcag cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt
    42001 gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc
    42061 ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga
    42121 aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc
    42181 aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga
    42241 tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt
    42301 ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa
    42361 aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa
    42421 cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa aaagagattc
    42481 tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga
    42541 atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag
    42601 aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa
    42661 acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt
    42721 atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga
    42781 gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga
    42841 agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc
    42901 tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag
    42961 aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat
    43021 tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga
    43081 gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa
    43141 acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc
    43201 cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa
    43261 gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag
    43321 caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt
    43381 tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt
    43441 tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg
    43501 gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt
    43561 aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt
    43621 atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg
    43681 cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt
    43741 tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag
    43801 aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca
    43861 gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac
    43921 caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca
    43981 cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg
    44041 aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg
    44101 cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca
    44161 aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca
    44221 tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg
    44281 atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg
    44341 atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag
    44401 ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt
    44461 atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca
    44521 cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat
    44581 acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat
    44641 aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc
    44701 gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca
    44761 ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa
    44821 tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag
    44881 ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag
    44941 aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac
    45001 ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct
    45061 gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat
    45121 acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg
    45181 acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat
    45241 gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa
    45301 tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg
    45361 gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct
    45421 gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct
    45481 ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg
    45541 gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt
    45601 caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta
    45661 aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca
    45721 attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa
    45781 cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc
    45841 acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt atttgaatta
    45901 aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga agacctttca
    45961 gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg
    46021 ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga
    46081 acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat
    46141 gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg
    46201 aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat
    46261 gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt
    46321 ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc
    46381 tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta
    46441 ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca cagaacgagt
    46501 attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg
    46561 tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca
    46621 gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg
    46681 tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc
    46741 gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg
    46801 aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca
    46861 taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc
    46921 tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt atgttccgga
    46981 acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt
    47041 aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc
    47101 agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc
    47161 ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat
    47221 cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa
    47281 atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat
    47341 cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt
    47401 ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg
    47461 gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag
    47521 caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc
    47581 aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag
    47641 aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag
    47701 atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa
    47761 cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg
    47821 tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat
    47881 gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg
    47941 aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc
    48001 atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa
    48061 caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa ttagacggag
    48121 ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa
    48181 aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg
    48241 gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat
    48301 ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg
    48361 cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg
    48421 ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg
    48481 gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa
    48541 atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt
    48601 gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca
    48661 ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt
    48721 ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt
    48781 ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta
    48841 gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt
    48901 gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct
    48961 tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct
    49021 aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa
    49081 atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt
    49141 ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa
    49201 atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt
    49261 aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc
    49321 gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa
    49381 acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca
    49441 tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct
    49501 atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc
    49561 gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagctgc agcagcagaa
    49621 caattaggat ttgctgacaa attcacacac atctcaacag gtggtggcgc aagcttggaa
    49681 ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag
    49741 tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat gactccctag
    49801 ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa
    49861 ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa
    49921 aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga
    49981 attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga
    50041 aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca
    50101 ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat
    50161 caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgcggcga
    50221 atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc
    50281 tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt atgaaccaat
    50341 ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt
    50401 tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca
    50461 atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga
    50521 cggtgcttta gtcggtggcg caagcttgca agcagattca tttttgtcat tgttagacgc
    50581 tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa
    50641 attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac
    50701 aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc
    50761 caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt
    50821 caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt
    50881 atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa
    50941 tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg
    51001 gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg
    51061 cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca
    51121 caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact
    51181 caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc
    51241 tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag
    51301 caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt
    51361 ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc
    51421 ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag
    51481 ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag
    51541 tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac tgggacggat
    51601 tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg
    51661 taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta
    51721 tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag
    51781 aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct
    51841 ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac tggttctcta tcacgtactg
    51901 accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag
    51961 aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag
    52021 atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc
    52081 tctacttgct gaaatatcag caggtagaga gt
//
LOCUS       DADQRX010000009        50249 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.009, whole genome shotgun sequence.
ACCESSION   DADQRX010000009 DADQRX010000000
VERSION     DADQRX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.009"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            13..645
                     /locus_tag="J8V14_000505"
     CDS             13..645
                     /locus_tag="J8V14_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920638.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(779..904)
                     /locus_tag="J8V14_000506"
     CDS             complement(779..904)
                     /locus_tag="J8V14_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920639.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1038..1931)
                     /locus_tag="J8V14_000507"
     CDS             complement(1038..1931)
                     /locus_tag="J8V14_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBA0920640.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2113..3039)
                     /locus_tag="J8V14_000508"
     CDS             complement(2113..3039)
                     /locus_tag="J8V14_000508"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBA0920641.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3124..3885)
                     /gene="pflA"
                     /locus_tag="J8V14_000509"
     CDS             complement(3124..3885)
                     /gene="pflA"
                     /locus_tag="J8V14_000509"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBA0920642.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4129..6360)
                     /gene="pflB"
                     /locus_tag="J8V14_000510"
     CDS             complement(4129..6360)
                     /gene="pflB"
                     /locus_tag="J8V14_000510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA0920643.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6637..9087)
                     /gene="parC"
                     /locus_tag="J8V14_000511"
     CDS             complement(6637..9087)
                     /gene="parC"
                     /locus_tag="J8V14_000511"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBA0920644.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9099..11144)
                     /gene="parE"
                     /locus_tag="J8V14_000512"
     CDS             complement(9099..11144)
                     /gene="parE"
                     /locus_tag="J8V14_000512"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBA0920645.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11310..11744)
                     /locus_tag="J8V14_000513"
     CDS             complement(11310..11744)
                     /locus_tag="J8V14_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBA0920646.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11871..12509
                     /gene="plsY"
                     /locus_tag="J8V14_000514"
     CDS             11871..12509
                     /gene="plsY"
                     /locus_tag="J8V14_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBA0920647.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12641..13516)
                     /locus_tag="J8V14_000515"
     CDS             complement(12641..13516)
                     /locus_tag="J8V14_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBA0920648.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13598..14389)
                     /gene="codY"
                     /locus_tag="J8V14_000516"
     CDS             complement(13598..14389)
                     /gene="codY"
                     /locus_tag="J8V14_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBA0920649.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14407..15807)
                     /gene="hslU"
                     /locus_tag="J8V14_000517"
     CDS             complement(14407..15807)
                     /gene="hslU"
                     /locus_tag="J8V14_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBA0920650.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15821..16369)
                     /gene="hslV"
                     /locus_tag="J8V14_000518"
     CDS             complement(15821..16369)
                     /gene="hslV"
                     /locus_tag="J8V14_000518"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBA0920651.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16406..17311)
                     /gene="xerC"
                     /locus_tag="J8V14_000519"
     CDS             complement(16406..17311)
                     /gene="xerC"
                     /locus_tag="J8V14_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBA0920652.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17509..18885)
                     /gene="trmFO"
                     /locus_tag="J8V14_000520"
     CDS             complement(17509..18885)
                     /gene="trmFO"
                     /locus_tag="J8V14_000520"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBA0920653.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18854..20932)
                     /gene="topA"
                     /locus_tag="J8V14_000521"
     CDS             complement(18854..20932)
                     /gene="topA"
                     /locus_tag="J8V14_000521"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBA0920654.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21054..21911)
                     /gene="dprA"
                     /locus_tag="J8V14_000522"
     CDS             complement(21054..21911)
                     /gene="dprA"
                     /locus_tag="J8V14_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBA0920655.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21967..22734)
                     /locus_tag="J8V14_000523"
     CDS             complement(21967..22734)
                     /locus_tag="J8V14_000523"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBA0920656.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22727..23587)
                     /gene="ylqF"
                     /locus_tag="J8V14_000524"
     CDS             complement(22727..23587)
                     /gene="ylqF"
                     /locus_tag="J8V14_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBA0920657.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23847..24245
                     /locus_tag="J8V14_000525"
     CDS             23847..24245
                     /locus_tag="J8V14_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBA0920658.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24482..25024
                     /locus_tag="J8V14_000526"
     CDS             24482..25024
                     /locus_tag="J8V14_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBA0920659.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="J8V14_000527"
     CDS             complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="J8V14_000527"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA0920660.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25810..26037)
                     /locus_tag="J8V14_000528"
     CDS             complement(25810..26037)
                     /locus_tag="J8V14_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBA0920661.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="J8V14_000529"
     CDS             complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="J8V14_000529"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA0920662.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26572..27285)
                     /locus_tag="J8V14_000530"
     CDS             complement(26572..27285)
                     /locus_tag="J8V14_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBA0920663.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27288..28184)
                     /locus_tag="J8V14_000531"
     CDS             complement(27288..28184)
                     /locus_tag="J8V14_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBA0920664.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28342..29184)
                     /locus_tag="J8V14_000532"
     CDS             complement(28342..29184)
                     /locus_tag="J8V14_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBA0920665.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29316..29969
                     /locus_tag="J8V14_000533"
     CDS             29316..29969
                     /locus_tag="J8V14_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBA0920666.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30032..30559)
                     /locus_tag="J8V14_000534"
     CDS             complement(30032..30559)
                     /locus_tag="J8V14_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBA0920667.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30579..31526)
                     /locus_tag="J8V14_000535"
     CDS             complement(30579..31526)
                     /locus_tag="J8V14_000535"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBA0920668.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31538..33424)
                     /locus_tag="J8V14_000536"
     CDS             complement(31538..33424)
                     /locus_tag="J8V14_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBA0920669.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33584..35662)
                     /locus_tag="J8V14_000537"
     CDS             complement(33584..35662)
                     /locus_tag="J8V14_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBA0920670.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35675..35950)
                     /locus_tag="J8V14_000538"
     CDS             complement(35675..35950)
                     /locus_tag="J8V14_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920671.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36312..36767)
                     /locus_tag="J8V14_000539"
     CDS             complement(36312..36767)
                     /locus_tag="J8V14_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920672.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36921..38129)
                     /locus_tag="J8V14_000540"
     CDS             complement(36921..38129)
                     /locus_tag="J8V14_000540"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBA0920673.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38241..38573)
                     /locus_tag="J8V14_000541"
     CDS             complement(38241..38573)
                     /locus_tag="J8V14_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBA0920674.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38721..39596
                     /locus_tag="J8V14_000542"
     CDS             38721..39596
                     /locus_tag="J8V14_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBA0920675.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39678..40259)
                     /locus_tag="J8V14_000543"
     CDS             complement(39678..40259)
                     /locus_tag="J8V14_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HBA0920676.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40265..41524)
                     /locus_tag="J8V14_000544"
     CDS             complement(40265..41524)
                     /locus_tag="J8V14_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBA0920677.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41526..42572)
                     /locus_tag="J8V14_000545"
     CDS             complement(41526..42572)
                     /locus_tag="J8V14_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920678.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42807..43082)
                     /locus_tag="J8V14_000546"
     CDS             complement(42807..43082)
                     /locus_tag="J8V14_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBA0920679.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43325..44635)
                     /locus_tag="J8V14_000547"
     CDS             complement(43325..44635)
                     /locus_tag="J8V14_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBA0920680.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44817..46046)
                     /gene="rpsA"
                     /locus_tag="J8V14_000548"
     CDS             complement(44817..46046)
                     /gene="rpsA"
                     /locus_tag="J8V14_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBA0920681.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46181..46861)
                     /locus_tag="J8V14_000549"
     CDS             complement(46181..46861)
                     /locus_tag="J8V14_000549"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBA0920682.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46910..47545)
                     /locus_tag="J8V14_000550"
     CDS             complement(46910..47545)
                     /locus_tag="J8V14_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBA0920683.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47611..49014)
                     /locus_tag="J8V14_000551"
     CDS             complement(47611..49014)
                     /locus_tag="J8V14_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBA0920684.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49001..50032)
                     /locus_tag="J8V14_000552"
     CDS             complement(49001..50032)
                     /locus_tag="J8V14_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920685.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50063..>50249
                     /locus_tag="J8V14_000553"
     CDS             50063..>50249
                     /locus_tag="J8V14_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBA0920686.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKC"
BASE COUNT        13579 a        10587 c         8617 g        17466 t
ORIGIN      
        1 tttggagata ttatggaaat tcaacgtaga accactaatt ttttacattt aggctctttt
       61 tttgcaactt tgacgatctt attttttcta tgttattcat accttaagac ctcacatgcg
      121 ccttctagtg cattaggctg gttgcttttg gatcattctg atatcacaga agccgaagaa
      181 aatccgtttt ttttgatttt gtcttctctt tgcggcttac tgttttctgt ttatttcggt
      241 gcctattttt ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt attgattgca
      301 tttaaaacgt tttctttttt agcaattctt ttttattcgt ttaatcacaa tacgcttttc
      361 ttctttatca tgaatggttt agtcgtgatt ttatccattt ttacttattt tattttttca
      421 ctcattttta gccaattgaa atgtccgctc ttctttcgat tagttcctgt cacctgtttt
      481 atttacagct tgttttcctt ttttgtcctt gctttaatgc cactgaccaa cccttcgacg
      541 atccaagtcg caggaaatct gttggatatg ctcttcatcg tcagtctagg tgtatttatg
      601 tggcaaagaa aaaacaaaca aaataaaaag cattttgaag cctgactatt acgacttcat
      661 aaatgctttt ttagttttaa acacttcaat ctaaaagaat aacccaagat tggataaatt
      721 attaatgact cgccattcgt ttcttagtga ttctggatta tcaaatggac ttcctaatct
      781 aatcagctag ttcttcccat aaaatgccat acttgactga atcataatat tgattattta
      841 aaaatctaac ttttcttatc actacttcct ttgtcattcc acatttctca ccattttttt
      901 gcattccttt gtttccagac catgtagtga atctaatagg tggtagatag gcaaaatact
      961 caaataattc ttttaaccat aaaggaagag aaaggctcct cctgcaagag tcagtataat
     1021 ttttgcttac tgctctctta ggttttactc actaaaactg ttccatattc tttgatataa
     1081 gaaaatgcat gcggtataaa tttcttgctc agctcagaaa agatttgtcg tttaagaaag
     1141 gctatttcct ccattgtcaa tgaaggatcc aatgttggta attcaatctc aaaattgttt
     1201 ttttcgacaa taggtaattc taatgtgaat gattcttctt cctttatagt attcgtcaca
     1261 agtagagact gatggaaaat actaggccgg cgatttttat atttattctt acgtatcctg
     1321 tcaacgataa cttctatttg atcaaaaaag tacgattcat ttacgtctcc aataagttcg
     1381 gctaattctt tgaatgcctt cgttacagga agctgattct tctgtacaaa aaagtaatta
     1441 gccaaagtaa gcgggaagtg ttctatagag gaaagtgtga taaaacggta cttctcattt
     1501 cctgcagttg tcctcagcaa ttctgtctct cggctgtttc ttactccata aaaatacgct
     1561 tctttccgtt catcttcgaa acaatacgcc caaataactt ctcccatcgc taatttttgt
     1621 tcatcaacag agagtctctt caattgttca gctatggttt cagcagccga agtggcttgt
     1681 tgcaagtaat ctttaggatc aaatattgga aagttcaaat gatatcttcg ttcttccctc
     1741 ttgattaatc ctgcatcgat caataaatca agaaaatggg aaaatttatt ttgcgaaaat
     1801 tcttttttca attctcttag aatcgtttct tgttctcgat gttcatatag ataatcggtc
     1861 aatgaagaaa aaagcggatc atcggtcagt ttttgataaa agttttcctt attttttgaa
     1921 taatagttca tatatgttct cttttctatc cgttttattt tacttaaaag gcttttacgt
     1981 aaaaaggagg aagaacaaga gtcataattg actgttattc tcctccattt cttcggcctt
     2041 tagtatgttc tatgtttctt tttatatcaa tcgcttatcc attcattttg aatgcttcat
     2101 actaccattt gattagctaa atacagccgt caattgaggt acgacttgtt ttttacgtga
     2161 aacgacacct ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag cttcttctac
     2221 tttttctttt cctttaccag caacaagtaa ttctgaatca ctgtctaaga tgtttgtgac
     2281 aactaatacg aacagatcgt agccatttgc tttcatttca tcttccattg ctgcttctag
     2341 ctctgcttgt cgatccatca cttcattaag gtccactgta ttgacttgag cgatacgaac
     2401 gttggaactg cccattggga aactttttgc atccaaatca agaagaaccg cagctgattt
     2461 atcacttaga tttgttcctg ctttcaacat ttcaagtcca taagtgttta gatcgacttc
     2521 agcgattgct gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg ttggtgattt
     2581 gaacaacaat gtatctgaaa tgatagctga gagcatgatt cctgcgattt ctttaggaat
     2641 tgttacttgg ttttctttat atagttttaa aataatcgta cttgtacatc caactggttc
     2701 tgcacgatag tacaatggat tagctgtttc aaagtttgca atacgatggt ggtctactac
     2761 agctaaaata tttacttcgc tgatatctga aatactttgt tggaattcat tatgatccac
     2821 aagcatgaca tctgcatttt ctgctttagc tgactctact actcgtggtg cagatagctg
     2881 aaagtggtct aatgcgtatt gtgtttcttc attaggtgta cctagagcaa ctgcttctgt
     2941 ttctttacct aattcatttt gcaagtgtga aaacgcaatg gctgccccga ttgcatctgt
     3001 atctggattt tggtgaccaa aaactaaaat ttttgacatg tcattcgctc ctttgtgatt
     3061 tctcctattt aataatagca aaaaaacgga aagaagcaat acttatcttc tttccgatgc
     3121 gacctatcgc gttaaatatt ttgtatactc ttctgtatga agcaatcgtt tagcattttc
     3181 gacacgttct tttgtaggtg gttcgatccc ttctaatgga tatttcaaac caagtgtttc
     3241 ccacttgtat tttcccatcg tatgataagg aagaatctct actctgtcta catttgacaa
     3301 ggtttgaata aagttgtcta gtcggatcaa atattcatca taatcacttc gctcaggaac
     3361 tagtacatgg cgaatccaaa ccggtttgtt gatttttgac aaatattgag ccatttctaa
     3421 aatattttca ttgtcccaat gcgttagttt tttatgctct tcattatcga tgtgtttgat
     3481 gtcaaataac agtaaatctg tatacttcat caattcttga aaacgtgaaa agaaaggttc
     3541 ttcatacgta aaaggttttc cgcatgtatc caatgtagta tgcatcttcc gctcttttgc
     3601 ttttttaaat agttcgatca aaaaatcgat ctgcaataga ggttctccac cactaacagt
     3661 aataccacct ttatctcccc agtaaggtct gaagcgttct gctttatcta aaagttcatc
     3721 tgctgtgtat tctttccctc cgccaatatt ccaagtatct ggattgtgac aaaattggca
     3781 acgcatgcgg catccttgca taaacacgac aaagcgcagt ccaggtccgt ctactgaacc
     3841 gaaagtttcg atagaatgaa catagccgat tgttttttct tccatttaag ttcacatctt
     3901 ttctttgatt tcattttaag tacaagttag ctgtcgaagc gaagctttct gaacaaaatt
     3961 tttcgcaatt gccgcttcgt tatgtgtaaa atttacaggg acggaacata caagtcctgc
     4021 ccctgtaaat cgtttctttc aaataagcat actgattatt cgattttgat gcgatggcta
     4081 ttcaacgtcc tttcgacgct aagtcgaaaa acttttttca cgaacttttt acattctgtc
     4141 gtgtgaagtt ctcatcaaga catctaattg ttgttctctt gtcaagtcac ggaatttaac
     4201 tgcataacca gatacccgga tcgtcaagtt cggatatttc attggatttt ccattgcatc
     4261 acgcaataaa tcattactga atacattgac attcaaatga taagctcctt ttgagaagta
     4321 tccatctaag acattacgca agttttcgat tcttgtatct tgttcttttc caagacctga
     4381 aggattgatt gtttgggtat ttgaaattcc atccaatgca cagccgtaat tcattttagc
     4441 tgttgagttc aggctagcta ataacccact ttgttccgca ttgtaacttg gatttgctcc
     4501 tggcgcaagt ggttttccag cttcacggcc atcaggagta tttcctgtat ttttaccgta
     4561 aacaacattt gaagtaatcg tcaataatga agttgttggt tttgagttgc ggtaagtttt
     4621 atgacgtttg atttgtccca tgaagtattc caagatccat gaaactaatt catctgcacg
     4681 gtcatcatca tttccgtatt tagggaagtc gccatccact tcaaaatcaa ctgctattcc
     4741 tttttcatta cggattgctt ttacttttgc atatttgatc gctgacaagc tgtctgccgc
     4801 atgagagatc ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat ccatcaaagc
     4861 cagctgagct gcttcataag aatatttatc atgcatataa tgaatgatgt tcaacgtatt
     4921 gacatatagt tctgctaacc aatccatcat attcatgaag ttatgtttca cttcttcaaa
     4981 gtctagtatt tctgatgtga ttggacgata ttctggtcca acttgggcgc cagtcacttc
     5041 atcgactccg ccattgatgg catataatag tgttttagcc aagttagcac gagcgccaaa
     5101 gaattgcatg tcttttccga caactgttcc tgatacacag caagcaatcg cacaatcatc
     5161 agaaccccat tgttcacgaa gctgattatc attttcgaat tgaatcgaag aactttcgat
     5221 agcgattctg ctagcatatg ttctgaatcc ttctggaagg tgttcagaat acaaaacagt
     5281 caagtttggt tctggtgaag gtcccatatt tgttaatgta tgaagcagac ggaaatcatt
     5341 ctttgtaact aatgaacgac catctaatcc catacctgca atggataaag tagcccagat
     5401 agggaatcct gagaataatt ggttatattc aggtgtacgc gcgaatttga ccatacgtaa
     5461 tttcattact aagtggtcga tcaattcttg tgcttctttt tctgtgatta ctccattatc
     5521 caaatcacgt tggatataga tatcaaggaa tgctgagata cgtccgattg acatcgcagc
     5581 accattttgt gatttgattg ctgctaaata tccgaagtat agccattgga cagcttcttt
     5641 tgcatttgca gctggtttag aaatatcata gccatatttc gcagccattt ctttcatttg
     5701 cgctaatgca cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt cttcactcat
     5761 tgtatgataa ccagtcatat catgatcttt tttcttttgt tccatcaaat agtcgattcc
     5821 atacaatgcc acacgacgat agtcaccgat gatacgtccg cgaccgtaag cgtctggaag
     5881 ccctgtaatg attttgtttt tacgagcagc acgcatttca gcagtataag cgtcaaatac
     5941 accttgatta tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat cgtttggtgt
     6001 atagccattg ctttttagcg cattgttagc catattgatt ccgccgaacg gcatgaatgc
     6061 ttgtttcaaa ggtttgtctg tctgtagacc gacgattttt tccaaatctt tatccaaata
     6121 gcctgcatcg tgtgaagtaa tagttgatac gatttctgtg tccatatcat agacaccgtt
     6181 acgctcgaat tggatatcat ttaacttttg caattcatcc cataattttt ctgttgcttc
     6241 tgttgggcct tccaaaaagc tgtcatcacc gcgatattca gtgtagttat tttgaataaa
     6301 gtctctagtg tctacgcctt ctttccattt ggtaccttta aatccattcc attgctccat
     6361 tgtatggacc tcctaaattt ctgttttatg taacagtttt tacgtgatta gtataacaca
     6421 gcaccctatc tttgcaaggg ttttcttgtt ttttatttaa caatttttat gttttattta
     6481 catatattta aatcaacgtt gacataccaa catttaagca ttgtgaacac tttcatcttt
     6541 ttttgcaaac gtataaggat ttattaaaaa tgaatgaaaa agagacgcca cctgtattaa
     6601 tacagatggc gtctcttcat taaaatacta caacagttac gactcgatga aaatctcagg
     6661 agcatcgata acttcgatga cttctccacc ttttttctca tccatcgcaa atgatccatt
     6721 cgatgttcga tcacctatag ggaaatcagt tgggtcaatc gttaactgct gccctttttg
     6781 attcatcaag gttatttctt ttgatcttcc ttccgacata tagataatac ggtgcggatt
     6841 ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt ggctgatttc
     6901 ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg attacgatcg gcgtatcacc
     6961 ttctgctaac acaagcagcc cgttgataat cctatctcca tctttcaaat tcatagactt
     7021 cactccagct gcctttgctc caacaacggg aacatctgtt aaaggatagc gcaaacccat
     7081 tccttgatgc gttactaaga aaacatctaa gcctggctga tcttctttca gatacacagc
     7141 aatcagttca tcttcttccg cctttagctt catacagctg agcggacggc tcttataggt
     7201 gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc aatccattct tgctgatgaa
     7261 tacaaatgtt ttctctgctt gaattttttg atatgggaaa acagctaaaa tcgattcgtc
     7321 tacagacaga ttggagattg tttgagaaat gtgctcacca gcatctttcc atttcaaatc
     7381 gggtagttca tggacagggc ggtagatcac atttgcttta tttgtgatca gaagaacatg
     7441 gtccaatgta ctcaattcac ctgagtatag caagtagtcg ccttctcgca tcccgatttc
     7501 ttctggtttg gaagcactat aagaacgaag gcttgttcgc ttcacatagc cttctcgcgt
     7561 cacactgaca attacgtctt cttgtgccac taagacttgc gtatcaattt tgatttcttc
     7621 gatttcgtct tcgattgtcg ttagacgagc ggaagaataa ttttttttca cttctcgcaa
     7681 ttcttttttc ataacagaga aaagctcttt gtcatttgaa aggattttat tcaactctgt
     7741 gatttgcgca atcaagcttt cggattcctt ttgaagctct gtgatatccg tgttcgtcaa
     7801 acggtataat tgcaatgtga caattgcttc tgcttgttct tctgtaaatt gaaagacgtc
     7861 gactaaattc tttttggcgt cttttttgtc tttactttca cgaatcgttg cgattacttc
     7921 atccagaatc gataaagctt tcatcaatcc ttctacgata tgctgtcttt tttgagcttt
     7981 ggctaaatcg aattgactac gttttgttat gacttgcttt cgatgctcga tataacttct
     8041 taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga ccatattgaa
     8101 attgtaattg atctgtagct cagtgttttt gaacaaataa ttcaaaattc cttgagcatt
     8161 ggcgtctttt ttcaattcga cgacaatttg cagacctgtt ctatcacttt catctcgtac
     8221 ttcagctatt ccatcgattt tcttattcaa acggatctca tccatttttt tcaccaaggt
     8281 cgctttattt acttcatatg ggatttctgt gatcacgatt tgctgtttat tacccttcat
     8341 tgcctctatc tttgttttcg aacgaagaat aacttttcct cgtcctgttt cataagcttt
     8401 tttgatttca tcttttcctt gcaagatccc tcctgttgga aagtcaggtc caggaatata
     8461 ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc atatagattg tgccatcgat
     8521 cacttcagct aagttatgag tcgggatttc tgttgcatat ccagcagata tacctgtcga
     8581 tccattgacg agaaggtttg ggtatcgagc tggaaggact gttggttctt tttctgtatc
     8641 atcaaaattc cacacgagat cgactgtttc tttctcgata tctgctagca tctctccact
     8701 taacttggat aatctggctt cagtataacg catcgcagca ggtggatcac cgtccatact
     8761 cccgttattc ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt ggctcatgcg
     8821 gaccatcgct tcataaatac tgatgtcacc atgaggatga taattcccca tgatatttcc
     8881 gacagatttt gctgatttac ggaatccttt gtcatacgta ttgccatctt tgttcatggc
     8941 aaataatatc cggcgttgca ctggcttcaa tccatcacgg atatcaggca gtgcacgttc
     9001 ttggataata tatttggaat aacgtccgaa gcggtcaccc atcacttctt ctaaagttaa
     9061 ttcttgtact tcttgtcgat tttccatcag gtgacctcct attctacttc aaataactga
     9121 ttgttttcat ttttatctgc tcgttcttca tctaataaat cattagaaac tgaaggagag
     9181 atttcagtat cctctttttt atctaagata ctgccatctt cttcgagcgt aaattgcacg
     9241 tgattttcga tccattttct tcgaggttct actttgtcac ccatcagtgt ggttactcga
     9301 cgttcagctt gcgcagcatc atcgatacgg acgcggatca acgtacggga agtcggatcc
     9361 atcgttgttt cccacaattg ttccgcattc atctctccaa gtcctttata tcgctgcagc
     9421 atatatcctt tgccgacttt tttaatcatt gctgccaatt cgtcatctgt ccacgcatat
     9481 tcaatgactt gcttctttcc ttgtcctttg gatactttat ataaaggcgg taacgcgata
     9541 tatactttcc cagcttcaat cagcggtttc atataacgat aaaagaaagt aagcagtaat
     9601 acttgaatat gggcgccgtc tgtatccgca tcggtcatga tgattacttt gtcgtaatta
     9661 cagtcttcga tagaaaactc aggaccgact cctgcaccaa tcgtatagat catcgtattg
     9721 atttcttcat ttttcaagat atcttgcatt ttagcttttt cggtattgat gacttttcct
     9781 cgtaaaggta gaattgcttg gaactttctg tctcttcctt gtttagcaga tccgccagca
     9841 gaatcccctt cgactaaata caattcattt ttcttagggt ttcgggattg tgcgggtgtc
     9901 aattttcctg aaagaagaga ttcgcctttt ttacgcttct tcccatttcg gctttcttct
     9961 ctggctttac gtgctgcttc tctcgcttct ctggctttga ttgctttacg gaccaacatc
    10021 tggctcattt cgctattttc ctgcaaataa aagcccattt gttcactgat gacgttatct
    10081 actacggtac gagcaactgg cgtaccaagt ttttcttttg tttgcccttc aaattgcaga
    10141 agattttcag gtacacggat agaaagtaca gcagctaacc cttctctgaa atcactacct
    10201 tccagattct tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc attataagat
    10261 ttcgtcatcg ccgctttcat accagcttca tgggttcccc catcttttgt ccgaacgtta
    10321 ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt attgataagc gacctctact
    10381 tcgatacctt ctttttctcc tgaaaaataa acaactggtg tcaatgtatc tttttcttca
    10441 ttcagataat caacaaattc tttgattcct tcttcgtaat gaaaaatttc cttgactggt
    10501 tcttctccac gaagatcaga aagagagatt ttaacgcctt ttagaagaaa ggcagattct
    10561 cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg tactgaatat tgtatcatct
    10621 ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt ttcctatctt tttcaaagta
    10681 ccgtcaggtt taccgccgtt tttaaatttc tgctgatact ctacgccatc tctaacgatc
    10741 gttacaatta gccatttcga taaggcattt accacactgg ctcctacacc gtgcagtcca
    10801 ccagaagttt tgtaccctcc ttgaccaaac tttccgccag catgcagcac agtaaaaatc
    10861 acttcaactg ttggtattcc ggaagcatgc attcctacag gcatcccacg tccgctatct
    10921 gcaacagtta tactattgtc ttcatggatg gtcacatcaa tctcactgcc ataacctgat
    10981 aatgcttcgt caactgcatt atcgacgatt tcatagacta aatgatgcaa gcctcggctg
    11041 tcagtggatc caatgtacat ccctggtctt tttctaactg cctctaatcc ttccaatact
    11101 tggatggacg agtcattgta ttcgttgttt actttttttg ccaaggcaaa cgctccttca
    11161 ataatacttt gcggcaaaac cgcaccacga ttatcatact agaaaaggtc gtgaaaaaaa
    11221 agagggtaga cgagaaatca attttaatta tctcctaacc gctttttcga cattcccctc
    11281 tatcgatcat ctcttctttg tggtcatttt tatttttggt tttctttcaa cttaccaagt
    11341 tcaatcttaa tacaacgatc cattacgata tctgtacgtc ctgcttttct tagcatctca
    11401 gctgcttctt tgttttcaat ccctaattgt gcccagaaca ctttagcatc agttttcaaa
    11461 aaatcacctg ctatttcagg taaaaattca ctccgtctaa atacatctac gatatcgatt
    11521 ggatcatcga tttctgtcaa attcgcatag actttctcgc ctagaatttc ttcaccagcc
    11581 aataatggat ttactggtat gattttatat ccattgtctt gcaatacctg tgcaaccatg
    11641 taacttgttc gttctggttt attgctcaag cctacgacgg caatattttt tgcttcttcc
    11701 aaatagttga aaatcgtttc ttgtgttgga ttttttacag acatttctgc caccccttgc
    11761 ttttcttttt attccatcta ttatacttgt atctttcatt ttttcaattg ttcctgttac
    11821 ctattcatgc taaaataaag gatagttttc aacttgaaag agggtaaaaa atgaaattga
    11881 tcttattact tattttagca tatcttttag gttctattcc ttccggtgtc tggattggca
    11941 aattgttttt caaaaaagat atccgacaac acgggagcgg taatactgga acgaccaata
    12001 catttcgtgt attaggcaaa acagccggga ttgttgtcct gcttatggat attttaaaag
    12061 gtacattggc tacttgtttg ccgatgatct tccacctttc tgggattaac cctctttggt
    12121 ttggggtatg cgccattctt ggccatacat ttccgatttt tgctgggttc aaaggaggaa
    12181 aagctgttgc gacaagtgca ggaatgcttt taggatttaa tcctgtattt tttgtttatt
    12241 cttgtatcat ctttatcgtt agtttatttt gtacaagtat ggtgagtttg agcagtatga
    12301 tcagtgcagt attgatcact ttatctacga tcgtccttcc ttatgtagct cctgtgattt
    12361 tggctaagcc caattggtta ctgacgatca ttgccttttt agtttcttcc tttatctttt
    12421 atcggcataa agacaatatc aagcgtatca gaaaccatac tgaaagtcga atcccgtttg
    12481 gtttgaatag cagcaagaaa aaatcctgat actttttcat tccaaagaga taaaacggga
    12541 gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt tacacacctt
    12601 ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact gtgatggaat
    12661 attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt tccatcaagt
    12721 ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa acgaaaggcg
    12781 cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag cttaaagtta
    12841 cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt gtttcaaaaa
    12901 ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt gtctgaccta
    12961 acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat cttgatttca
    13021 atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga ggtacattga
    13081 aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt gccacttgat
    13141 aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca aaatcaaatg
    13201 gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt tcttgtccat
    13261 gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt tgatatgttt
    13321 tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat aaaaccggtg
    13381 catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca tgttcttttg
    13441 atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc tggtcatttt
    13501 gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa tgggaaggct
    13561 ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt tttgctaact
    13621 cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct aacgaacgag
    13681 attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca gaacgagtga
    13741 tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca tccaatgctt
    13801 caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt gccatttgga
    13861 cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa aggatctgca
    13921 taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca aacgattgtt
    13981 ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc ggaacgacag
    14041 tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta atatcactag
    14101 taattgggat attagcttca gttttcatca actgtgccac tgtatcagta taagattcag
    14161 gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg tgacgttcat
    14221 tataacctag taatactccg tcacttccta tgatataggt gttgctgtca agaataccac
    14281 ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa gtattctttt
    14341 gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt gtcgtcctcc
    14401 gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc caagtttgtc
    14461 atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg cttcaaataa
    14521 tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga tattatctgt
    14581 gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt ttgtaaacgt
    14641 aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag cattatttgg
    14701 ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta cccgtatcgg
    14761 gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga aagctcctga
    14821 agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta cttgtgaacc
    14881 ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc cagattgctg
    14941 tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt cttccgctaa
    15001 tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt cttccttgat
    15061 caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct tcggtgtcaa
    15121 agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt tcatcattgg
    15181 tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca gtaaaccctt
    15241 ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca cttcttcttg
    15301 cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt caaattgatt
    15361 agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca aacgtttttc
    15421 tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta cgatttggat
    15481 cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat aaccgacttc
    15541 agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg ccaaacgtcg
    15601 tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt ttggtgtaat
    15661 atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca aagcaacggc
    15721 aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac ttacgatttg
    15781 tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct tctacgataa
    15841 tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt tctgcaattt
    15901 ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca gccaacgcaa
    15961 aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact tctcctgtac
    16021 ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca agtttttgca
    16081 tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt agattcccgt
    16141 tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct gcaacacttc
    16201 cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt gcagtgcctt
    16261 tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca aatttcccgt
    16321 ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg tttgtttcct
    16381 cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga tagttttttt
    16441 gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg gcatgtccta
    16501 gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta gcaaatgtat
    16561 ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc atttgattca
    16621 agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca aaaacgatat
    16681 catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa tattcttgca
    16741 gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct ttaccatgga
    16801 tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta cactcactta
    16861 aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa tccaaaggac
    16921 tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa aaacgtggca
    16981 gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt atcacttctt
    17041 ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg atagaatttc
    17101 tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc gtaaaatatt
    17161 ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa tggagaatgt
    17221 cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc aaataacgca
    17281 aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt cccagagaat
    17341 aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc aatatttcta
    17401 agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt aagaaaaacg
    17461 caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt actcatttgg
    17521 gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag ctcgttgtgc
    17581 aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag gaaacagacc
    17641 atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt agtaagccat
    17701 acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg ctaagcgtgc
    17761 ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt ctacaccagt
    17821 catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg gtttcagcaa
    17881 ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga attcagcatt
    17941 ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca agtgagtctg
    18001 aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct ggataacagc
    18061 ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag gtccgaaaag
    18121 catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt caaaatattt
    18181 ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct catggaaagc
    18241 tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt tgtcatagcg
    18301 ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg gtgcagctgc
    18361 atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg ctaatggatg
    18421 agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag taatttcttc
    18481 gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt cagaaaaaga
    18541 atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg ccgaagtgat
    18601 tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat tagccaaact
    18661 gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt gatgcgcctc
    18721 agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt gtgcgatctg
    18781 ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt tcaatgaaag
    18841 aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca cagactactt
    18901 gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga caatttctgc
    18961 ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa ccatagaata
    19021 gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact gggcaagtca
    19081 caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta ctgcaagcat
    19141 aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca tcacaatcga
    19201 atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca ttcgtcagtt
    19261 ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc gtgccttctt
    19321 caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg atttgaggga
    19381 agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca aatctttttt
    19441 gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca taagtggaag
    19501 gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta aaacgtgctg
    19561 gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca tcgccttctt
    19621 caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct tccacataca
    19681 cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg acaccatttt
    19741 gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta gccacaaaac
    19801 gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt ttgagttcat
    19861 ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt gcgccttgag
    19921 catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta tcttcaataa
    19981 atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta cgcatgtatg
    20041 taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat tgctgtgcta
    20101 ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt tgcaggctac
    20161 ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact tttttaactt
    20221 cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca tctggtaatt
    20281 tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga gcttttttaa
    20341 aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct ttttcacggt
    20401 caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct ttcttgacct
    20461 tacgccagag aataggactg atcgaataac caacgattct gtctaaggta cgacgagctt
    20521 gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct gctttgactg
    20581 cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc aacccaagta
    20641 ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt gccaagtaaa
    20701 ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct tttccgcgta
    20761 tagaaatata atggggttca taattgtttt caaaatcgat acccatttta cttttcggta
    20821 aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga tatttctcaa
    20881 ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc attactgaat
    20941 ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa atcgtagcta
    21001 atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg gtgttattct
    21061 ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc ttggatcaaa
    21121 tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa aactgttctt
    21181 ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt ggcttcaaag
    21241 acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa acaatgcttt
    21301 ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc aatcacctct
    21361 tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat gactgcgatt
    21421 gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc tttcgcgcag
    21481 ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata aactgttttt
    21541 ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca agaaagtaaa
    21601 gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag cagctgaggg
    21661 ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt gccccagtta
    21721 gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat tttacctatc
    21781 ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca gttagcaagt
    21841 gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa attttcttct
    21901 atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg taaaagcggc
    21961 tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga ttggcgtaat
    22021 tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat tatgcgagaa
    22081 accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat atactttagc
    22141 aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta ttttttcctg
    22201 ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta caatggacaa
    22261 tttttctact gcttggatca tcgctaattt acttgcttca taaatattca catcatcgat
    22321 tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa cttgttgata
    22381 aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta atatgatctc
    22441 atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc ctcttcctac
    22501 ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt caaagcaaaa
    22561 catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt caaaagaagc
    22621 cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg taggatcatc
    22681 tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag tcatcacttt
    22741 tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct tgacgaatat
    22801 catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt tttgctgtaa
    22861 tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa ccataacgat
    22921 cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc gctaaatcat
    22981 ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg ccgatttctt
    23041 catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac tcattaccta
    23101 aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt gcaattttct
    23161 tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct aagaccattg
    23221 cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt tctttcaatg
    23281 cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc gtgatagcta
    23341 atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt tgtggatctg
    23401 ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc tgatctagca
    23461 ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg atgtccacat
    23521 atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga aaccattgga
    23581 tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta cttgtttttt
    23641 tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt agttaagtga
    23701 aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa atttttattt
    23761 tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag atgatagacg
    23821 aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat tttcctgttg
    23881 ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa aatgaagaat
    23941 tttctactga agaagctggt tcaatggtat gggatgacac tttttggata atgcttctct
    24001 ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat aaaaaaacaa
    24061 gtggtgcttt gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa
    24121 ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa
    24181 tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga
    24241 tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga
    24301 taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa
    24361 gcagtcttct cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt
    24421 catgacacat tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat
    24481 tttgaacact ttgattgcaa aagacgtttt tagattgccc gaaatgaatg caagattgtt
    24541 gtcgacctct gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc
    24601 tgcttatttt caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg
    24661 accgatctgg gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg
    24721 ttcaggaacg tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg
    24781 cttattttta tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat
    24841 tatgatccca gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga
    24901 tttcaaagta atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat
    24961 aatggttgtt tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt
    25021 ttgattttac gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct
    25081 caacagactg cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa
    25141 tagacaaatc ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta
    25201 tcttttgaaa ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat
    25261 tgtcccgtta gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat
    25321 ttcaggtttt tagttagata aggttcctta actactttcc cattgatctt caattgatca
    25381 ttttggtagc tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca
    25441 tctgctaatt gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca
    25501 accatatcac cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg
    25561 aataaaaatc cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca
    25621 agccagaagc gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt
    25681 tttttcattc tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa
    25741 taaaaataat tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa
    25801 aactatctct tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat
    25861 atccatggac ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct
    25921 agagctgtgc ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc
    25981 atcatgtgtg ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct
    26041 tcgttcctcc aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt
    26101 tcaggatgct ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct
    26161 ggttcgattt ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta
    26221 cttttttcag caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg
    26281 tcaccgcgat cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt
    26341 tctactaatt gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta
    26401 tgtccagtcg tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta
    26461 tatcctgaca tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag
    26521 aagcagcctc ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc
    26581 cgatgatttt ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt
    26641 atttgagttg tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc
    26701 atcatttact aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg
    26761 atccaaccta agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt
    26821 atagtttctc ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga
    26881 aagacttcta gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa
    26941 atagaaatcg ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc
    27001 caaataaaat ttatattcaa tatcactatc tttttgatac tcactaagga aaaagctgat
    27061 gatttgattg actttttctt tatttgaatt gatcgttaag acaggatctc cttgtctttc
    27121 cgtaagagct ggtatttctg ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa
    27181 aaatacggtc gaaccaagta ctaccccaag aaggataagg aaggcgaatt tccagtaatt
    27241 tgttttcttt gttttgtttg aagatacgtc agatcttgtt ttcatgttta ttgatcatcc
    27301 tccttctcta accattggtt ttgtgtctcg atcagctgat ctctcactgc atttgccatc
    27361 aattgataac ctaaattatt tggatggaaa tgatcttctt catagagcac attatttttg
    27421 atgccattta gattttctgt cgtgctagtt gtttgatctg ttccttcttc tgtgacacct
    27481 acttcctgat tcaatccttg ataaagcaaa tcattgattg ggataaagta gcagttcggc
    27541 attttttttg tcaccgtttc tgttcctttg ttccaattgt taacaattgt ctgcatgtca
    27601 gtgatctcag gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg
    27661 gttttattga gggatcgaaa ttcttccaat agttctgtga cattttcctg atagtttttc
    27721 agtggctttt taaaggaatt gaccgttaac ccgaataaat tattttggat aactttcatc
    27781 aaatcattac cgccaacagt cagtgtgata aaatcagctg ttttgatatt gtttcgaatt
    27841 tcttcttttg tttttactcg ctttaagatc tggtcacttc gttcaccatt tacaccatag
    27901 ttttctgttt caaccgcggt caaattataa cggtctttta gatcatttgc gacaagggga
    27961 acgaatcctc cttgcttggt ttgatctcct actccttcag tcagcgaatc tccgatagct
    28021 atgtagtgaa gtttttcttt tttatttgga tcttctctca cagtagttac tggtttcatt
    28081 agtgggccag ctttcggtat agaagcagct aaacctgcat agatgatgat cgcaattaat
    28141 aatgggaaca aaacgaggat caaatgtcgt ttcgttaatt tcattttttc acctcttgta
    28201 ttcgattggt tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta tgaaaaaaga
    28261 aaacaattat tttcctaatt ttataaagaa aaagagaata agctgcaagc ctttcaggct
    28321 gagggcttat cctcttttcg actaatctac ataatacatg attgcaaacg cgtctttacc
    28381 agtatgcgta gcgataaccg gattcgtatg cagtacaggg atatgcatgt ccgggaataa
    28441 ttcttgcaaa ccctctttga attcttgcgc caattctttt gcatccgcat gtgaaatacc
    28501 gatttgtcgc acatttggca attctttcag ttcatttttc aaattattaa accatttatc
    28561 gaacgttttc tttcctcgcc ccttagttac agggattaat tcagaatggg cgaaattcat
    28621 caccacgcgt atgttcaaaa cactagacag caaaccagtt gttcggctga ttcttccacc
    28681 ttttaccaag ttatctagtg tggatattcc gatgtataat tttgtattgt cttgtacttt
    28741 tttgacttct tcaaggattt tctctactgt tttaccttct tttgctaatt cagctgctcg
    28801 aattacctga aaagataatc cttggtcagt ggtatcactg tcgataaccg ttactttcgt
    28861 ttttgttaga ttactggctt gtcttgctgc ttctactgtt ccactcaagc cttttgtcat
    28921 atggatcgaa atcacttcac tgccgtcttc tcctagctca tcatacatcg agatgaattc
    28981 tccaattggt ggttggcttg ttttgggcag attttttgct ttcgccatca tttccataaa
    29041 tctttcgccg tctagttggt catcatcggg atatactgtt ccgtcgatca tgatggatag
    29101 cggcatgaca cgaatgccta aagcatcgcg tacatttttt tccatcgtac atgaagaatc
    29161 tgttacgatt tttacatttg tcattatttt ctctctctct ttctttaaca agatgcctta
    29221 ttcatgttaa actacatgta agacaatttc tatttttagt ataacagaca aataatgatt
    29281 tttcattaat tacacctagt aaaggagccg ttttcatgga aaaaacgaaa tttaatcgaa
    29341 cgtatgaaat attaaatgaa gtgtttaatg cagtcaccca tggaattggg actggattga
    29401 gtatcgcggg actagtggtt cttttagtaa aaggtgccca cttgcattcc tccctccata
    29461 ttgtctctta tgctatctat ggatcgatga tgattctttt atttttaacg tctactttgt
    29521 ttcattcatt gatttttaca aaagcaaaaa aggtgttcca agtattcgat catagttcga
    29581 tttttctttt gattgcagga agttatactc ctttttgttt gctgagtatc cgaggatggc
    29641 taggttggac tttatttgta ttaatctggt tgtttgcgat tcttggcatt atatataaag
    29701 cgttgacgtt gcataaacgg gagagagtat caaaagtatc tacgatcatc tatattttta
    29761 tggggtggct ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga atgactggtg
    29821 ttattttgat ggtagcaggc gggctttctt acacgatagg cgcattcttt tatagcttga
    29881 attctatccg ctatatgcat gttgtttggc atttgtttgt tatgttaggt gcaggtttta
    29941 tgtacttctc tgttttattc tttacttaat aaaaaaaata aagctatgga tgggacagct
    30001 ctaaataaca gctagtctat ccatagcttt tttatatcgt caattcgcct gtcccgcttt
    30061 ttacaggatt ctcattacgc tgatacgttt caaattgatg cggataacga tttttttcat
    30121 ccatttctcc ttgactagta ttgatcagtg accaatcaga ccagtcaaca ggtggaaaat
    30181 aagcatctcc atcgaataca tgatggatga ctgtacggta taatatcgta caaaatggca
    30241 gaaattcttg ataaacagct gaccctccag cgatgaaaaa gataccgtct gtttcttttt
    30301 ccagagcaag cacgtcttcc acactatgca tgacatgcac gccttctgct ttataggaag
    30361 aatctctcgt caaaacaatc gtctgacgat tcggcaacgg acggctaccc atcccttcga
    30421 atgtttttcg tcccatcact agtgtatttg cttctgtcat ttgtttgaag aatttcagat
    30481 catttggcaa acgccaagga agacggtctt cttttccaat caatccgttt tcatcctgtg
    30541 cccaaattgc agctaacatt ttctcatcct ttctgattct aaacagcgat aggagctttg
    30601 atcgccggat gtgggtcata tccttctaat tggatatctt ccatctcaaa atcaaatact
    30661 gactgctttt cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg gctcaattgt
    30721 tctttcattt gttcgatatg attgatataa agatgagcat ctcctaatgt gtgaacaaat
    30781 tcgccaaccg ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc ataacttgca
    30841 atgttgaacg gaacgcctag aaacacatcc ccactgcgtt gatacaattg gcagcttagc
    30901 cgtccctctt gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc catgctagga
    30961 acatcttccg gattccatgc agaaacgatc aatcttctag aatcaggagt ctttttgatt
    31021 gcttcgatca cttgtttgat ttgatcgata aagccaccgt cttttgtttc ccagtgacgc
    31081 cattgatagc cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga atcatcggtt
    31141 aggatcttct cgcaaaaaag cttgctctgc tttttatatt cttcattgaa ttcaggatct
    31201 gtcaaacatc tatggccaaa atcagtcata tctggtccat tatagtctgg acttttgatg
    31261 tagcgttcaa atgcccattc atcccaaata tggtttttat tttgtaatag atagcggata
    31321 ttcgtatctc cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa aggtactcgt
    31381 ttcgttgtta acaaaggaaa ccctttttgc agatcaaacc gcatttggta tccgaataag
    31441 cttaatgtgc ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa caattttctt
    31501 cctaagtcaa gatacgcttg ttccatcctt tttcctccta atcttctacc cattcactca
    31561 agtattccca ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat acgtcgtttt
    31621 gcaattcttg taatcgagtg aaattatctc cttgatgatt catctcttct tggagcaatg
    31681 agattttttc ttcaagttct gcaatctcat cttcaatcgt ttcccattct ttttgttcca
    31741 tgtaagataa tttcttcttt tcagcaggtt ccttgctctg tatttttggt ttcgcttttt
    31801 cagcaggctg ctgggtctct ttttgcttca gtaaataact ggacatgttt ccgtaataaa
    31861 gttcttgttt tccgtttcct tggaatacta ataacttatc tgctaccttg tccaagaaat
    31921 aacggtcatg ggaaacagtg atcacagcac ctggaaaact tctaaaataa tcttccaaaa
    31981 tcgtcaatgt tgctatgtcc aaatcattgg tcggttcatc caataataaa acattaggtt
    32041 gctgaatcag cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa agtttgccta
    32101 tcagtgtccc atgcataaat cttggaaata aaaatcgttc aagtaattct gctactccga
    32161 tttgactgcc atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa gcaatcatcc
    32221 gtttatctgg tgccatttct tcattttctt gtgtataata cgcaagatga accgtttcac
    32281 cgatagacat tgtaccgcta tccaggggaa ttctgccagc tagaatatta agcaatgtcg
    32341 attttcccgc accattttta ccagtaatac ctagtctttc ccgagattga atcaataaat
    32401 ctaaatgttc tagcagtgtt tggtcattta tacggtaact gccatctttg atttccagta
    32461 cttttttccc taaacgttgt gtcgctaggt tcagctctaa atcatcttct tggtttactt
    32521 ggtacaagtt ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc ttggtgccac
    32581 gtgcctgtac gccagccctc atccaagcta attcctgttt ataaagccgc ttgcgctttt
    32641 cttcttgttc tacttctaca cgatcacgtt ctgctttttc taagacgtaa gcttcataat
    32701 ttcctttata ctcgtataat tttccaaatg aaagttcgaa gattcggttt gctactctat
    32761 ctagaaaata acgatcgtgg gtaaccatca atagtgcgcc acggtattgt ttcaaatagt
    32821 tttccaacca ctcgatcgct tcataatcta aatggtttgt tggttcgtct agtaatagta
    32881 aatcgggttc atcaatcaac acttgcgcca gactaactcg ttttttctga ccacctgata
    32941 aagtactgat ttttttatct aattcactga ttcctaattt ttgcaaaata atctttgcgt
    33001 tggtatctgt agtccaagca tcttctttat tcatttgttc ttctgcttta gcataacgtt
    33061 tttgcacatc agggtcttct ccgttttccc ctagttcgat caatgcttcc tcatatgctc
    33121 gaacagtttg gatcaagggg ctgtttcctt gaaagactgc ttggagaaca gttaattcat
    33181 cggaaaattc agctgcttga gaaagataac caatacggta atcagttgga taaaacactg
    33241 tttgccgatc accgtcgctg ctatcgatac cggcaataat atttaagaga cttgttttac
    33301 ccgtcccgtt cgtcccaatc aacccgatac ggtctttttc gtggaccaaa aaagaaatct
    33361 ggtcaaacaa atctttttca ccataagttt ttttgaggtc agttactttt aattctttca
    33421 acatgttccc tccttatctt ggatattgta tcataaaaat agtgaacttc aggaaaattt
    33481 ttttggtatt tgttcttttt gtgttctcaa aaaaagaaca ggaccgattc gatcctgttc
    33541 tgatatgttt tgttttttat aaaacttgaa aatttttacc tttttatttt attggactcg
    33601 ttttttttga ctttgctcga aacgcaaatc ggaaagaaaa gttatcagat ggtttgttca
    33661 tcgtataaga ttctaacaaa ttgatattcc tgcgatagct gttcgcagaa aacgtcgtga
    33721 ttttttcagc aacttcgtat tcatcaatga tttttctttc aagataataa cctcttagca
    33781 tttttttcgt aaacagacta tcttgctgga tcatccacga agaaatcaaa ttccccgagt
    33841 tcatttttat tccttcaagt ttgcgttgct ggataaaaaa ttgaaccatc gtttcatcat
    33901 aaatatcagt caagttatct aaactttcaa taatcaactg agtatttttt tgatatgtct
    33961 ctctgatttc ttggatatct tcatcactaa taaatgtctg acgatcagca tatcttcttt
    34021 gccaaaacat tttcggatgg agaacgattc gcattaacct acggacaaca atgaaccaaa
    34081 agccgataaa agacaaaatc tggcttgtga tagaatgttc gaatcgagaa agaaaacggg
    34141 agtaaaaacg ataaccatta gaagaaagct tgcccgaacg ataactttca tccaatccat
    34201 cccgttcgat cgacaaaatc agtgcttgta tttcttgtac ttcttgcttt tcatcatcgg
    34261 ttaaatcttc caagtacaga tcacgtatcc gttcttgata attttctatc actgcttcag
    34321 tggctaaaaa ttcttttgta ttacgatcaa tttcctttat ttcttttcgc aaaacttcaa
    34381 cgacctcttc taaaatcatc aaagcattca aatccgtgct tttcgcttcc tctgtttccg
    34441 tcagcatagg caagacaatg atccctatga ccaaagtcac taagatgaca cccgccgtca
    34501 gaaacaaaag caaagaccgc tggtaaaaca tcatattatt gattgaaaat ggcaaaataa
    34561 aaatcgtagc caaactgact gttcccttta caccgccaaa tgttaagatc aagatctcgt
    34621 tcaactgttt tttgaacttt ttactgccat ctttgaatac ataaaaaaga ctgatagaaa
    34681 tgaatctgat cacaaaaagc atgacagaga ttaatacgat aatcgctaat aaaagaccgt
    34741 ttggatataa accgtctccc catactggcg agaacacttg cgtcaattcg attcctaaaa
    34801 atataaatac taatgcattc agtgtaaaag caatagttgt ccatgtactg tgggacaagc
    34861 tcgacaattc tgcatcaaac acagagattt tcctaaaacc agaagcttgg agaatacctg
    34921 ctgccactgc agcaatgatt ccagatacat caaatgcctc tgccaataca taggctagaa
    34981 aaggaagcaa aagttcgatc agcagatacc ctgtgacatc ttgtgctgat gctttttcga
    35041 tcaaatgaat tattttttgt ttgaaccaga caagcaaaaa gccgatcaaa gctcccccaa
    35101 tactagaaac tactagactc atccccgcgt ttactgctga aaaagatccg gtgatcagcg
    35161 caccaagagc aaactggaag gcagtaacac cagatgcatc attcaaaaga ccttctcctt
    35221 ccaaaatatg catcgccttt ttagggatat tcactcgtcc agaaagagaa ctgactgcta
    35281 ccgcatctgt cggacctaac gctgctccaa aagcaaagca ggcagcaagt gggacacttg
    35341 gcaacaggaa tgataacgtc gcacctaccc cgacaagggt caaaatgact cctccaaatg
    35401 ccaagaacaa aattgtcccg aaatttttga gaatcgaaga aatatctgct ttttctcctt
    35461 ctctaaataa caatggtgca ataatcataa ctaagaacaa ttcaggttca aaatcgattg
    35521 attgtcccca ctctgtcaga cctaatacca ctccaaggaa aatttggatc agtggagctg
    35581 gaatcattgg caaaatgcgg ctagctatgt tcgataaggt gatcgttact gcaaatacaa
    35641 taactaactc agcaaattcc atcgtccatc ccctttattt tttattttgt gtaagcattt
    35701 ttaaaaattg ttcttttttc tttaaaattt tggtttcgac tacttcaacc tgtgtttggt
    35761 tcccctctaa ttcacactgt acacgttttg actccaaacg aagcagatca tgtttcagac
    35821 gttccaactg acgatacatc aatatatttc ttttacgcaa cttacaaagt atttgacggc
    35881 ggtcatccat cgaaagggcg ccatagcgat catgcaacag ttttttttgt tgttcaaaac
    35941 tctctctcat tttccagatc tcctatacac aaacttccat cattatagca aagttctttt
    36001 tggatgaaaa atatttcatt gtttcatcgt ttattgctag ttagaaattt ccataaattt
    36061 ttcaacgcct cattctattc caaatagtat cagatatcaa gtaaaactta agaaagataa
    36121 aagtttctga caaaaggatg tttactttcc cattcctgcc tttcttgttc ttttatcaca
    36181 acaaaaaaat ggggctgcga caaaaatctt gtcacagtcc cttattctga ataaacggtg
    36241 tggaagagac agcctatcag tagtaataag acacatctct ttcacagcct catttttgcg
    36301 taaaaatcga tttattcgaa aaattccccg tatacatctt ctacacgttg atctggattg
    36361 atcacgatat acaagttacc tgttttttcg ctgatttttg ttgattgata ttgattgtct
    36421 aatccttctg ttcctttatc tagatatggg aagtacacaa gatcaggtgt gccatttcgg
    36481 taaaggattt ccattcgctc gatatcttcg tatttcaatg cgcgattaaa catgcccatt
    36541 tccagtccgc ctaggttgat atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg
    36601 attttaaatc cagcacacgg atggatttca acaaattcac tgccattgat tcgtccaaaa
    36661 cttgttgtga tttgtttgat ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt
    36721 tctccatttc taaaataaaa ttttagcgct gtcatttctt tactcattaa aaagactcct
    36781 ttatgatgta atttattctt gtactacctc agtttagcac aaatttatgt aagtggtcac
    36841 atcttatgtt ttttcttcta atgacgtatc tgcccacttt aagtagccaa caaagctgag
    36901 cagatacagt ctagtgtttt ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt
    36961 tttttatcat tttttattct ttgttctacg acggcttttt cagctaattg caaagcttca
    37021 ctaacccact taccaggttt ttttccagtt tcttctagca agattttacc atctactgct
    37081 aattcgctta atgacttgat aggtaaacga tcataggatt ctcctacggc atccatctga
    37141 ggtgtttcgt caaaatagat catcaattct tctacttgca gtgcatgttt tttgcctaag
    37201 aagtacaatt cgaatggttc caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt
    37261 gcttgaacgt ctttaatcat ttggttagaa cacttccacg atttcaaaaa ggaacgaatc
    37321 tcattttctt ttagatccag ttggctgatc aataatgccc atgcttggct ctcttctttc
    37381 aatgggacgg atggtaattc agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg
    37441 ggacagcaaa tgtagcactc tgtttcaata aatgcatgga ttcccgcggc ccgttcattt
    37501 cccaagagca tcttgatgaa ttccacattt atacgttcta cagatatttt acctaatagc
    37561 tcgtggtttt ctttgatggc gtagaaggtt tcttcctcta atttgaaccc tagttgactg
    37621 acaaaacgca acccgcgcat cattcgtaag gcatcttcat ggaaccgctc atgaggattg
    37681 cctacagctc gcaagatctg ttcttctaga tcttgcagac catcgaacaa atcgatgatt
    37741 tctccatctt ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt caaatcttct
    37801 gccaacgaac gaacgaattc gacatgatct ggtctacggt agtcctgata agttgattcc
    37861 gtccgaaatg tggtgatctc atattgttca tcattttcta ataccaacac agttccatgt
    37921 tcgatcccaa tatctatcgt ttttggaaaa agctgtttga tttcttctgg aaatgcgctt
    37981 gttgcgatat ccacatcatg gattggctgg ttcagcaaga tatcacgaac acttccacct
    38041 acaaaataag cttcatatcc tgcattctct aattttttca gaactggaat tgccttttgg
    38101 tattccagag gtaattgttt taatttcatt tttccaacct ttcctgcgtg tttcttgtcc
    38161 tgagataaag actcagttgc atggtgactg tttgacaagt ccgtttcaga gtcttcaagg
    38221 ccaggaccac ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg gtctctttga
    38281 ttgaattttt ccatgttttt ttcaaaaact tcagttaagt cgatattcaa tgaattagcc
    38341 atgatcatcg taacgaataa aacatcacca agttcttctg ctaccgagtt aactggttca
    38401 gaagcctttt tgcttttttc tccataagtg tggttgactt ctcttgccaa ttcacctact
    38461 tcttcagtta atcgagccat ctgagctaat ggagagaaat accccacctt gaattgctgg
    38521 atatattcat ccacttcctg ctgcatactg tataaagaac gctttttatc catctttttc
    38581 cctccaaaac tatcttacca aaactttaac gcaaatccta gataagagca attgagaaaa
    38641 tcgaatttcc atgctacatt taggaaggaa tttctaaatg gaaattctgt gaaaatacat
    38701 aattgaaagc tggaattcat atggtcaaac gaaaaacagt aaaagaagtt ctttttatca
    38761 ttataggaac aagtatttat gcatttggtc ttgtttactt gaacatcgct aatcatttag
    38821 cagaaggggg agtctcaggg atcaccttga tcttaagagc tttatttggt attgatccag
    38881 cttatacgac tttattaatc aacatcccat tgattttact aggaggaaag atcctcggta
    38941 aacgttcctt ggcctatact gttctaggta cactttcttt gtcattcttt ttatggatct
    39001 ggcaaagggt tccactagaa atcaatttac agcatgatct actgatcgtt tctctacttg
    39061 cgggtttagt tgcaggtgta ggaagcggga tcgtctaccg catgggtgga acaacaggcg
    39121 gcagtgatat tatcgctcgg attttagaac aaaattacgg catcactatg gggcgttccc
    39181 tattggcatt tgatatcatc gtcttacttg cttcattaac ctatatcgac ttgaaacgta
    39241 tgatgtatac gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg atcttagatg
    39301 gcggatattc tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag attgcaccac
    39361 tactgatgac cggtctgcaa agaggtgtga ctttcctttc tggagaaggc gcattctctg
    39421 gtaattcaaa aaaaatcatc tatatggtcg ttagctctag tgaattaact gaagctaaga
    39481 ggatcgtcca cgagattgac gaaaaagctt tcctttcaat catcaatgtg catgaagtgg
    39541 aagggcaagg ctttacttat ttgaagccac agacaagatt actgaaaaaa ctataagaaa
    39601 aaagacaaag ataggctatg aggaaagatt ccacatccag ctgacctttg tcttttttag
    39661 tactatacta tttttaacta attgttatgt tcacttaccc ggttactttt cttttgtgtc
    39721 tgcaaaagga tctttcggtt aacttcatca gataatttca aaaaagcatc cctatctcca
    39781 tcttctagtg cttgatcgat ttgagcataa agcattttta gttttgcctc ttcttctttt
    39841 tcttgaaaat atcgttcaat taattcagta tcttcttcac tgactgaaga atcccaagaa
    39901 gaaaatggat tgtcttctaa gatagataga tattctcttg tctgccaagc attttcgaat
    39961 atacattcca catataaagg ctttttccaa ttcaaacgga tttcatgaaa aatctggtct
    40021 gtgtccgtaa attcttttcc ctgcaaaaac aatttcatgg gttccccttc aacagataga
    40081 tcactgatct gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg gacattgctt
    40141 aaaatcgctt catgattggc aagataattc aagatccaaa aaacttctct tcggctaaaa
    40201 gatacatgat tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc aattagcatt
    40261 tttctcacct ctctgttagg tcatctaata atgacagtgc ctccatatct cctggttcaa
    40321 gtgttaaata ataagtcaat aattcttgcg ctcttttcag ctgcccttct tctcttaaga
    40381 aaaaggcata ctctttcaag aaatctggtt catgtgatag ttcatgataa gcctgttcat
    40441 agtgtacagc tgcaacggta aagtcttcta attcattata agcatgggct aggttccatt
    40501 ctgcataagg attagcagtt tcttccatct gattgatcac ttgtatcaca tcttcatatc
    40561 tttcatcatc caagtaaaga ttacttaaag taagtaatgt ttcatcttgt ttgtctccta
    40621 cttctaaagc tttcagtaaa agttcttctg ctcgctgtct atcatgcaga cggaaagcat
    40681 tttccgaggc caggtgataa agttcgactt ggtaaggatt ttctttgatt ccttcttcta
    40741 acactgtttg tgcttcttca atcatttctt cttcttgaag agcttgtcct agatatagat
    40801 ataacgattg ataatgcgga ttaacttctc gaagttcttc cagatagttg attgcctgct
    40861 gattttcttt caattgaaga ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt
    40921 cttctttcag tgcttcttct aaaggtacga tagctgcttc gaattctcct tgcatactta
    40981 aggattgtcc taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca
    41041 acaatgtttc ataaaccact gctgcctctt tgaaacgatc aactgaaaaa tacaattcag
    41101 ctagcgcaaa ctggatcaat ggttcatcag gaagcaaccc agctgcttct ttcaattttg
    41161 cttcactcac ttcagggatt ccgataactt ggtacaaatc agctatcgcc aataagcttt
    41221 gcggatagta atcactttgc ttatccactt tttccaggta ttcaaaagct aagtcgatct
    41281 cattatcttc gattgcaatt tctgctaacg ggatgttcag tccgtcattt tctgggtatt
    41341 ggttgatcag ttgttcaaaa atctgttttg cttcgatcaa aaagccaata gccagcaatt
    41401 cttcgcctag ttcagctaat atatctgctg ggtcttcttt taatgctttt tctaaaaaga
    41461 tttgtgcttg tgacaagtct tcatctgata aagcttgcaa cattttttcg ctatttgtat
    41521 tcatttcaat tccctcttat ctcactgacg atttgttgaa ataccgtcat tgttctcaat
    41581 aattcttttt cgtttggatg ttggtatccg tctattatac ctattttttc taaaacaagt
    41641 atttttttgc ttttttctgc caaaaaaaga actttttcca aatactccat ctttgaaatc
    41701 tgttccagcc agtggattgg atagccaagc aagtccagcc acacaaaaaa gttttttagt
    41761 tggaaatcaa aaggatatgc ttggagattc cacacgaaat gaaacatcat tcctaaaaat
    41821 tttttcatac tagcagaaag tagatggcca ttttcggtca gataaaaagc ttgttcaaac
    41881 agtttaccat aatttgacag gttttctgcc tctaattgat aaaaatagat catttcttcg
    41941 atcatcccgg caaacggccg agtcgttaat tgctttttat ttggatagtt tttatacaga
    42001 ttttgcaaaa aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc
    42061 tgctttccgt ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga
    42121 ttgatggttt gcagcactac acgatttggt agctgcaaaa tcgtccgctg cggtgttaat
    42181 gctcggctta gagaacggat cgatacaggg atcatccata gtgatgaatc aagggttgta
    42241 tgtttttgaa aaaaacctgc cagatccatg atcccttcat ttccaaatcc gatgataaac
    42301 atggattgat tttcgggata gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa
    42361 tgattgcatt gagtgttggc acaaatatac cagtcattgt ttgcttgatt tgcaaataac
    42421 gggttgatct tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa taagattggc
    42481 tgatcaatgg aaagttctct tagctgactg actagcgttt ctccgtaaaa cactttcgtc
    42541 aacgcatctt ctacttgata atcaagttcc atacgcatcc ctcctcttct tatttttatt
    42601 ttaccattag taagcaaatt ctagaaaaga taagagcaaa gctcacctca taacttgcaa
    42661 ataaataaaa attaaataaa atacatgtgt tttctatgta tgacatacgg attcggaatc
    42721 tctgtctctt atttattcct tttctgctag gaaataaaaa gaaaagcact tgtagcccta
    42781 gagccacaag cgctttttca actgtcttat ttaacagcgt cttttaatgc tttacctggt
    42841 ttgaatgcag gtactttgct agctggaatt tcgatttctt caccagtttg tgggttacga
    42901 cctttgcgag ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa ttgaactttt
    42961 tcgccgttag ctaaagcatc ttgaatagtt gaaaatacag catctactgc tgcagttgca
    43021 tcttttttag ttaaatcagt agctgaagca actttttcga ttaattctgc tttgtttgcc
    43081 atggatagat tcacctcctc cacaagagat atgatagaaa tatcatttag ttaaaacttt
    43141 ctgagtggtc cagaagttta aaaagaaata ttgattaact tcaatattct gttatcacga
    43201 tatcatatga accgcttaat agcaaggttt ttaccaattt tcacatgctt tttttagtca
    43261 tagaaacgta acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa
    43321 atgcctattt tctcctacga gggataattc ggatggacgt cccttcaaaa gtaaatgctt
    43381 ttctgatttg attttctaag aaacgttcat aagaaaaatg catcaattcc tcttcattga
    43441 caaaaacgac aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa atcttcaggc
    43501 gttttccttt atctgttggt gttggattga ttgctacagc atccataatg atatcattca
    43561 acacagccga agagatacga agattctggt tcatacttac ttgttcgatc atttcaggca
    43621 gacgttccaa ccgttgttta gtcactgcag aaacaaaaac gattggagcg taatcaagat
    43681 accggaattc atcacgaatc tcttgttcga aatcacgcat tgtattggtt tctttcttta
    43741 caagatccca tttattcacg acgataataa ttccacgacc agcttcatgg gcgtaacccg
    43801 ctactcgctt gtcttgttcc cgaatccctt cttcagcatt cagtaccatc aaaacgatat
    43861 cagaacggtc aatggcacgc attgcacgca tcacgctgta tttttcagta ttttcgtata
    43921 cttttccgcg tttacgcata ccagctgtat ctatcattaa gaatttctga ccactttcag
    43981 aaacgaagtg ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa acaattacac
    44041 gttcttctcc taaaatcgca ttgatcaaag aggatttacc aacatttggg cgaccaatca
    44101 aactaaactt aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa tacttcactg
    44161 cttcatctaa aatatcacca atccctagtc catggcttcc tgaaacagga taaggatcgc
    44221 ctagccctaa ggaataaaat tcatagatat cgtttctcat ttctggatta tctactttat
    44281 tgactgctaa aatcactggt ttattacttc tgtataaaat tttcgcaacc atctcatctg
    44341 cgtctgtcac gccttcgcgt ccgctgacta cacaaatgat tacatctgct tcttcaatcg
    44401 cgatttctgc ttgatgcttg atctgatcca taaaaggttc atcgccaaga tcaattccgc
    44461 ctgtgtcgat cacgctgaat tctcgaccta accattcacc ttttgcataa atacgatcac
    44521 gagttactcc aggagtatct tcgacaatcg agatacgctc accagcgatg cggttaaaaa
    44581 tcgtagactt accgacgttc gggcggccaa cgattgcaat tgttggattt gccatattat
    44641 ttcctcctca caagtttcca atctcactta gtttaacgaa tgcattgcca actttcaagc
    44701 atagattgtc aattaaacga agtattttta ttccttattg aaatactaat cgcttttcaa
    44761 aagaaaaact gagacaaaag cagctgcttt catctcagtc acagatacaa aatcaattat
    44821 ttttcagaat catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg
    44881 tctcccatag taaatccagt attttcttct ggtaattcat aagactcaac attttttggt
    44941 tcttcttctg aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct
    45001 tctggatgga tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt
    45061 ggtgtcgcaa tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg
    45121 aacacttcaa caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg
    45181 ccaactggag cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac
    45241 aaggaaacac gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg
    45301 acagccaata catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact
    45361 agaccgtcaa ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt
    45421 ccactgagaa catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt
    45481 tcgttttcca ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg
    45541 atgattttaa agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct
    45601 tccaccattg atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg
    45661 acaacatttg taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct
    45721 tcccaaactt ttttagcatc taaacggcgt tttgaaagta gatagctgcc gttttcttta
    45781 tctttgccga tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg
    45841 ctgatatctt ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca
    45901 gccccttcga ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca
    45961 acatcgccaa cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc
    46021 tcttgatttt gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa
    46081 gtacggtact gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt
    46141 ttccttgaaa aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt
    46201 ttttcaaaac aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat
    46261 cgatacgaac agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat
    46321 ggtctctttc ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt
    46381 ggttctcttt gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca
    46441 cttctgcatt cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc
    46501 gttcccctat ttttcgttgg acagcaacta attcttctct gacttttcca tgtgcagaga
    46561 cttcagaaac agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg
    46621 ctatgaatac atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca
    46681 gcgaaactag cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta
    46741 ctgcgcgata catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct
    46801 tggcaacggt acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca
    46861 tgatataact cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc
    46921 ttaattcttg accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca
    46981 attgatcagt ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa
    47041 cagtcgttgt tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc
    47101 ttgaatcccc tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa
    47161 tactgctttc tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac
    47221 tagattctac tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta
    47281 ctactgatgt cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc
    47341 tgatccagaa ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata
    47401 gcgaaagaaa taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt
    47461 catcatctgt atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag
    47521 attgatgtct atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata
    47581 ccataggaaa aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt
    47641 tagtaaaatc gttccccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag
    47701 aagttctgct tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc
    47761 gccaaaataa gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat
    47821 ctgctgcagg cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc
    47881 tggtttttca gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc
    47941 ttgctctgtg gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg
    48001 attaaagaaa taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc
    48061 ttcctgatct ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa
    48121 atcataatgg accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt
    48181 agcaatgaga aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc
    48241 tgcatcaaga ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa
    48301 tcgttcgact tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat
    48361 gaagtgttca agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg
    48421 ccgattgact gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc
    48481 tcttgctact aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag
    48541 ctttcggatt tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac
    48601 gcaatgagcc tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag
    48661 agaaggatta gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg
    48721 gaggacgtat cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc
    48781 ccctcgtttt tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat
    48841 gatgaccaga ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc
    48901 tgtaggcaaa atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg
    48961 gccgggacga aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta
    49021 tcgcttcctt tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc
    49081 catctacatt ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac
    49141 gttcgattgt ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc
    49201 actcaataag atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt
    49261 caattgtctg accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt
    49321 gctgtaataa tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg
    49381 ctaacaaaag gtggatacat ctgctttgtt gcagataaac cgctgactca tcattgtttt
    49441 ttgccagctg ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa
    49501 aatctgctgc ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat
    49561 caatcaaaat ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat
    49621 aaaaaggact tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta
    49681 cttgaacagc aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa
    49741 aacgcaaacc agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta
    49801 gctttgcttc attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt
    49861 gataaaactt atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg
    49921 taaatccata aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac
    49981 taatccgcag cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct
    50041 ccttaagtta ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat
    50101 tgcatgcggt ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat
    50161 cgttttattt gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac
    50221 atatgtaaaa aaagcgtatc aaaaatgtc
//
LOCUS       DADQRX010000010        50103 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.010, whole genome shotgun sequence.
ACCESSION   DADQRX010000010 DADQRX010000000
VERSION     DADQRX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.010"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..293)
                     /locus_tag="J8V14_000554"
     CDS             complement(<1..293)
                     /locus_tag="J8V14_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA0920687.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYG"
     gene            complement(295..492)
                     /locus_tag="J8V14_000555"
     CDS             complement(295..492)
                     /locus_tag="J8V14_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HBA0920688.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(886..2613)
                     /locus_tag="J8V14_000556"
     CDS             complement(886..2613)
                     /locus_tag="J8V14_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0920689.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2606..3799)
                     /locus_tag="J8V14_000557"
     CDS             complement(2606..3799)
                     /locus_tag="J8V14_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA0920690.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3986..4630)
                     /locus_tag="J8V14_000558"
     CDS             complement(3986..4630)
                     /locus_tag="J8V14_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0920691.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4724..4858)
                     /locus_tag="J8V14_000559"
     CDS             complement(4724..4858)
                     /locus_tag="J8V14_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBA0920692.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4860..6992)
                     /gene="feoB"
                     /locus_tag="J8V14_000560"
     CDS             complement(4860..6992)
                     /gene="feoB"
                     /locus_tag="J8V14_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBA0920693.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6989..7462)
                     /locus_tag="J8V14_000561"
     CDS             complement(6989..7462)
                     /locus_tag="J8V14_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBA0920694.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7863..8087
                     /gene="nrdH"
                     /locus_tag="J8V14_000562"
     CDS             7863..8087
                     /gene="nrdH"
                     /locus_tag="J8V14_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBA0920695.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8246..10405
                     /gene="nrdE"
                     /locus_tag="J8V14_000563"
     CDS             8246..10405
                     /gene="nrdE"
                     /locus_tag="J8V14_000563"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBA0920696.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10455..11420
                     /gene="nrdF"
                     /locus_tag="J8V14_000564"
     CDS             10455..11420
                     /gene="nrdF"
                     /locus_tag="J8V14_000564"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBA0920697.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11509..12648)
                     /locus_tag="J8V14_000565"
     CDS             complement(11509..12648)
                     /locus_tag="J8V14_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA0920698.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12957..13670)
                     /locus_tag="J8V14_000566"
     CDS             complement(12957..13670)
                     /locus_tag="J8V14_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBA0920699.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13924..14625)
                     /gene="nagB"
                     /locus_tag="J8V14_000567"
     CDS             complement(13924..14625)
                     /gene="nagB"
                     /locus_tag="J8V14_000567"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA0920700.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14859..16391
                     /locus_tag="J8V14_000568"
     CDS             14859..16391
                     /locus_tag="J8V14_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBA0920701.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16582..17784)
                     /locus_tag="J8V14_000569"
     CDS             complement(16582..17784)
                     /locus_tag="J8V14_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0920702.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17866..18555)
                     /locus_tag="J8V14_000570"
     CDS             complement(17866..18555)
                     /locus_tag="J8V14_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA0920703.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18661..19029)
                     /gene="rplL"
                     /locus_tag="J8V14_000571"
     CDS             complement(18661..19029)
                     /gene="rplL"
                     /locus_tag="J8V14_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBA0920704.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19082..19585)
                     /locus_tag="J8V14_000572"
     CDS             complement(19082..19585)
                     /locus_tag="J8V14_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBA0920705.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19995..20684)
                     /gene="rplA"
                     /locus_tag="J8V14_000573"
     CDS             complement(19995..20684)
                     /gene="rplA"
                     /locus_tag="J8V14_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBA0920706.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20788..21210)
                     /gene="rplK"
                     /locus_tag="J8V14_000574"
     CDS             complement(20788..21210)
                     /gene="rplK"
                     /locus_tag="J8V14_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBA0920707.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21553..22221
                     /gene="sdaAB"
                     /locus_tag="J8V14_000575"
     CDS             21553..22221
                     /gene="sdaAB"
                     /locus_tag="J8V14_000575"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HBA0920708.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22234..23106
                     /gene="sdaAA"
                     /locus_tag="J8V14_000576"
     CDS             22234..23106
                     /gene="sdaAA"
                     /locus_tag="J8V14_000576"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBA0920709.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23197..24216)
                     /locus_tag="J8V14_000577"
     CDS             complement(23197..24216)
                     /locus_tag="J8V14_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HBA0920710.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(24389..25276)
                     /locus_tag="J8V14_000578"
     CDS             complement(24389..25276)
                     /locus_tag="J8V14_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBA0920711.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25353..25898)
                     /gene="nusG"
                     /locus_tag="J8V14_000579"
     CDS             complement(25353..25898)
                     /gene="nusG"
                     /locus_tag="J8V14_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBA0920712.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25999..26169)
                     /gene="secE"
                     /locus_tag="J8V14_000580"
     CDS             complement(25999..26169)
                     /gene="secE"
                     /locus_tag="J8V14_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBA0920713.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26193..26345)
                     /gene="rpmG"
                     /locus_tag="J8V14_000581"
     CDS             complement(26193..26345)
                     /gene="rpmG"
                     /locus_tag="J8V14_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBA0920714.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26507..27151
                     /locus_tag="J8V14_000582"
     CDS             26507..27151
                     /locus_tag="J8V14_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBA0920715.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27207..27710)
                     /locus_tag="J8V14_000583"
     CDS             complement(27207..27710)
                     /locus_tag="J8V14_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBA0920716.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27723..27769)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28307..29272
                     /locus_tag="J8V14_000584"
     CDS             28307..29272
                     /locus_tag="J8V14_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBA0920717.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29371..30543)
                     /locus_tag="J8V14_000585"
     CDS             complement(29371..30543)
                     /locus_tag="J8V14_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920718.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30530..31675)
                     /locus_tag="J8V14_000586"
     CDS             complement(30530..31675)
                     /locus_tag="J8V14_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920719.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31689..32613)
                     /locus_tag="J8V14_000587"
                     /pseudo
     CDS             complement(31689..32613)
                     /locus_tag="J8V14_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32730..33809
                     /locus_tag="J8V14_000588"
     CDS             32730..33809
                     /locus_tag="J8V14_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBA0920720.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33841..34434
                     /locus_tag="J8V14_000589"
     CDS             33841..34434
                     /locus_tag="J8V14_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920721.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34544..34771)
                     /locus_tag="J8V14_000590"
     CDS             complement(34544..34771)
                     /locus_tag="J8V14_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA0920722.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34874..37462)
                     /locus_tag="J8V14_000591"
     CDS             complement(34874..37462)
                     /locus_tag="J8V14_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBA0920723.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37482..38141)
                     /locus_tag="J8V14_000592"
     CDS             complement(37482..38141)
                     /locus_tag="J8V14_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA0920724.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38190..38693)
                     /gene="trmL"
                     /locus_tag="J8V14_000593"
     CDS             complement(38190..38693)
                     /gene="trmL"
                     /locus_tag="J8V14_000593"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBA0920725.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38914..39780)
                     /locus_tag="J8V14_000594"
     CDS             complement(38914..39780)
                     /locus_tag="J8V14_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA0920726.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39889..40530
                     /locus_tag="J8V14_000595"
     CDS             39889..40530
                     /locus_tag="J8V14_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBA0920727.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40592..41953)
                     /gene="mgtE"
                     /locus_tag="J8V14_000596"
     CDS             complement(40592..41953)
                     /gene="mgtE"
                     /locus_tag="J8V14_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBA0920728.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41971..42870)
                     /locus_tag="J8V14_000597"
     CDS             complement(41971..42870)
                     /locus_tag="J8V14_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBA0920729.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42872..43669)
                     /locus_tag="J8V14_000598"
     CDS             complement(42872..43669)
                     /locus_tag="J8V14_000598"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBA0920730.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43647..44351)
                     /locus_tag="J8V14_000599"
     CDS             complement(43647..44351)
                     /locus_tag="J8V14_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBA0920731.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44482..45058
                     /locus_tag="J8V14_000600"
                     /pseudo
     CDS             44482..45058
                     /locus_tag="J8V14_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45176..45853
                     /locus_tag="J8V14_000601"
     CDS             45176..45853
                     /locus_tag="J8V14_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBA0920732.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45932..47740)
                     /gene="pepF"
                     /locus_tag="J8V14_000602"
     CDS             complement(45932..47740)
                     /gene="pepF"
                     /locus_tag="J8V14_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBA0920733.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47916..48914)
                     /locus_tag="J8V14_000603"
     CDS             complement(47916..48914)
                     /locus_tag="J8V14_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBA0920734.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49140..49793)
                     /locus_tag="J8V14_000604"
     CDS             complement(49140..49793)
                     /locus_tag="J8V14_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBA0920735.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15002 a        10511 c         8781 g        15809 t
ORIGIN      
        1 ccataatctg ctcgatataa accatttatt ccagcagttc ttccaatttc actcaaattt
       61 cttattaatt cctcttgata ttcagtgaat cttattttat tagtataagc agtaaaatat
      121 agataattca ccaatctatt tattaaagga ccgggatcta attcatattt ttgtttttct
      181 aattttaaat ataccttttg taaaacctca agaatatcag aatatttagg atctttactt
      241 atagatatat cattatataa ttcatgaaca atttgcttag catttttttt cattttagca
      301 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
      361 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      421 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      481 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      541 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      601 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
      661 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      721 cgcacccata aattcataat agtttgtcgg cgtctttgga cgtttcgaag atgagctacg
      781 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      841 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      901 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      961 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
     1021 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
     1081 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
     1141 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
     1201 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
     1261 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
     1321 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
     1381 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
     1441 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
     1501 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
     1561 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1621 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1681 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1741 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1801 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1861 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1921 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1981 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     2041 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     2101 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     2161 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     2221 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     2281 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     2341 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     2401 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     2461 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     2521 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     2581 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2641 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2701 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2761 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2821 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2881 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2941 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     3001 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     3061 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     3121 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     3181 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     3241 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     3301 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     3361 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     3421 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     3481 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     3541 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3601 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3661 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3721 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3781 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3841 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3901 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3961 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     4021 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     4081 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     4141 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     4201 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     4261 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     4321 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     4381 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     4441 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     4501 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     4561 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4621 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4681 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4741 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4801 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4861 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4921 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4981 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     5041 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     5101 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     5161 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     5221 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     5281 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     5341 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     5401 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     5461 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     5521 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     5581 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5641 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5701 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5761 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5821 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5881 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5941 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     6001 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     6061 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     6121 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     6181 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     6241 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     6301 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     6361 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     6421 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     6481 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     6541 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6601 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6661 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6721 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6781 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6841 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6901 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6961 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     7021 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     7081 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     7141 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     7201 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     7261 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     7321 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     7381 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     7441 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     7501 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     7561 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7621 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7681 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7741 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7801 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7861 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7921 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7981 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     8041 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     8101 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     8161 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     8221 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     8281 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     8341 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttctatc
     8401 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     8461 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     8521 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     8581 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8641 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8701 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8761 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8821 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8881 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8941 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     9001 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     9061 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     9121 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     9181 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     9241 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     9301 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     9361 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     9421 tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat gtaacttagg
     9481 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     9541 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9601 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9661 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9721 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9781 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9841 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9901 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9961 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
    10021 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
    10081 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
    10141 tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg tcgatcaagg
    10201 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
    10261 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
    10321 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
    10381 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
    10441 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
    10501 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
    10561 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10621 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10681 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10741 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10801 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10861 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10921 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10981 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    11041 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    11101 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    11161 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    11221 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    11281 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    11341 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    11401 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    11461 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    11521 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    11581 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11641 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11701 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11761 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11821 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11881 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11941 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    12001 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    12061 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    12121 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    12181 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    12241 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    12301 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    12361 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    12421 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    12481 ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca ttttcaatat
    12541 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12601 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12661 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12721 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12781 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12841 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12901 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12961 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    13021 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    13081 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    13141 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    13201 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    13261 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    13321 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    13381 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    13441 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    13501 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    13561 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13621 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13681 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13741 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13801 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13861 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13921 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13981 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    14041 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    14101 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    14161 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    14221 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    14281 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    14341 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    14401 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    14461 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    14521 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    14581 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14641 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14701 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14761 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14821 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14881 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14941 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    15001 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    15061 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    15121 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    15181 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    15241 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    15301 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    15361 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    15421 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    15481 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    15541 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15601 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15661 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15721 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15781 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15841 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15901 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15961 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    16021 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    16081 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    16141 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    16201 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    16261 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    16321 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    16381 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    16441 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    16501 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    16561 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16621 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16681 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16741 gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta gcccctgtac
    16801 tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa
    16861 aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat
    16921 aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat
    16981 aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc
    17041 ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt
    17101 tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa
    17161 atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga
    17221 acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa
    17281 atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa
    17341 aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc
    17401 cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa
    17461 aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac
    17521 ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg
    17581 aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac
    17641 tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata
    17701 catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa
    17761 aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta
    17821 tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg
    17881 ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt
    17941 aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc
    18001 acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg
    18061 tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat
    18121 catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca
    18181 atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt
    18241 gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct
    18301 caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc
    18361 tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag
    18421 ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata
    18481 caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata tcaatcggat
    18541 tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac
    18601 ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa
    18661 ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc
    18721 ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc
    18781 tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc
    18841 agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc
    18901 agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac
    18961 taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt
    19021 caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca
    19081 attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc
    19141 ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt
    19201 ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga
    19261 tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat
    19321 cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc
    19381 cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag
    19441 catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa
    19501 cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt
    19561 tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag
    19621 ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca
    19681 tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc
    19741 gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa
    19801 aaaactctat gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg
    19861 gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca
    19921 actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac
    19981 attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt
    20041 gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat
    20101 acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg
    20161 attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact
    20221 tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag
    20281 ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca
    20341 acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct
    20401 acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa
    20461 acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct
    20521 gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca
    20581 atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca
    20641 acttttttta atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga
    20701 ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg
    20761 agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt
    20821 tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa
    20881 ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc
    20941 gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt
    21001 tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc
    21061 tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc
    21121 ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa
    21181 tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg
    21241 ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa
    21301 ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat
    21361 ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa
    21421 caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat
    21481 gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat
    21541 gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg
    21601 atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt
    21661 agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa
    21721 acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc
    21781 gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg
    21841 tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa
    21901 ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct
    21961 gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac
    22021 caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat
    22081 atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa
    22141 gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac
    22201 agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta
    22261 gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg
    22321 agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa
    22381 caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat
    22441 gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta
    22501 aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt
    22561 gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat
    22621 cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg
    22681 ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt
    22741 ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat
    22801 caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc
    22861 gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct
    22921 tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc
    22981 gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa
    23041 ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg
    23101 gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac
    23161 gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtatattt
    23221 tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag
    23281 gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg
    23341 tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg
    23401 acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg
    23461 caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat tggataccca
    23521 gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt
    23581 tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc
    23641 ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact
    23701 ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac
    23761 gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc
    23821 tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa
    23881 ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc
    23941 ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc
    24001 ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa
    24061 ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg
    24121 catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga
    24181 accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag
    24241 ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt
    24301 agatccttct ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc
    24361 aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct
    24421 gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt
    24481 tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta
    24541 acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc
    24601 gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga
    24661 cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat
    24721 taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat
    24781 acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt
    24841 tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat
    24901 tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc
    24961 cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt
    25021 ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc
    25081 cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt
    25141 ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac
    25201 ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt
    25261 tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc
    25321 caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa
    25381 tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt
    25441 tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc
    25501 acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc
    25561 aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa
    25621 ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg
    25681 atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca
    25741 tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt
    25801 ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat
    25861 gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt
    25921 aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag
    25981 tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc
    26041 cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt
    26101 acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag
    26161 aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat
    26221 tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg
    26281 actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt
    26341 gccatttact tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta
    26401 actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat
    26461 gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac
    26521 acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc
    26581 attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa
    26641 tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga
    26701 tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac
    26761 ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag
    26821 tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc
    26881 ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga
    26941 tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct
    27001 tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga
    27061 agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga
    27121 aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat
    27181 ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct
    27241 aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg
    27301 ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc
    27361 atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg
    27421 ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca
    27481 tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa
    27541 agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc
    27601 ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg
    27661 ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct
    27721 cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac
    27781 aataattata acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa
    27841 aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa
    27901 ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg
    27961 tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg
    28021 tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta
    28081 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
    28141 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
    28201 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
    28261 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
    28321 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
    28381 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
    28441 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
    28501 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
    28561 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
    28621 agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
    28681 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
    28741 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
    28801 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
    28861 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
    28921 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
    28981 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
    29041 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
    29101 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
    29161 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
    29221 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
    29281 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
    29341 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
    29401 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
    29461 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
    29521 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
    29581 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
    29641 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
    29701 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
    29761 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
    29821 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
    29881 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta gattgatgac
    29941 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
    30001 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
    30061 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
    30121 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
    30181 atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt gttcggtaaa
    30241 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
    30301 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
    30361 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
    30421 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
    30481 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
    30541 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
    30601 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca
    30661 aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa
    30721 tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca
    30781 ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca
    30841 atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc
    30901 aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata atataaatct
    30961 ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc
    31021 taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat
    31081 tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa
    31141 aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa
    31201 aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta
    31261 taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg
    31321 cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat
    31381 ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt
    31441 tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat
    31501 ccgctttgat tacaattcca gtattaatcg tttcgagatt gccaccttga tcaatctcgc
    31561 caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca
    31621 tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt
    31681 cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac
    31741 gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc
    31801 ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg
    31861 attccatcag aagctattgt tcgctgacaa cacgttttct gggagtaatg ggatgtcgat
    31921 cgtcaaacga tccgccgtcc gtaccatatc ttttagagat tctttggttc ctttagcaat
    31981 cacctgtcct tgatccatga tcacgatctg gtcgcaaaga atttctactt cttccatgta
    32041 atgtgttgta tagacaatgg ttgctccttg tcgattcagc tccttgatac tttctaaaat
    32101 tttattccga ctttgggggt ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg
    32161 cttgtgggca atcccacatg cgatgttcaa ccgacgtttc aagccaccac taagttctcc
    32221 tggacgaaac gaaacatatt tttctaatcc cactaattga atcacctctt caatcaaggc
    32281 tttccttttc tctttttcgg aaacatataa accgcaaaaa taatcgatat tttcttgcac
    32341 acttagttcg tcaaagaccg caacgtcttg aggaacgacc ccaatcattg ccttgacagc
    32401 ataattttca ggagtcattg ttgtcccgaa caatttaatg gttcctttgt cataatttaa
    32461 caaagaaagc atacagttga tggcagtcga tttcccactg ccgtttggtc ctaacagacc
    32521 taagacttct ccttcttgta cttgcagatc aaaatgattc aatgctacaa aatctccata
    32581 gcgtttgact aatttatcaa tttcgataat catcatgaaa aactccttcc aaatttcttg
    32641 ttgatctgac tataataaag tgcaagaaaa gaaactagtg acgattggct aaaccacaca
    32701 tgacatttgt cacaatgaag gagtctgcta tgcataaatc tcggctactc aacgaaacta
    32761 gctttctact ggttatctca ttgcttttgc tggtaagtca aggattcgtt gctatgcaag
    32821 ttatctattt attgatcgcc gttttattca ctggtgtcag ttatttactg cctacaaagc
    32881 caaattggat tttccaagtt attctatttc ttttatgtgc ctttttacct gatcttgtcc
    32941 tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga aagccacctt gattacttgt
    33001 ttttgttagg ttttttgttg atttcgacgg ttggtaatca tccccccatc aaggtcgcct
    33061 ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg
    33121 atcttgagaa aaagtacatt gtctccaaag atactcattg ggaaaaacaa caagagttat
    33181 cggaacaaaa taaagcattg gccctgtcac aagaaacaca attagccttg caaattgtgg
    33241 aagaacggaa ccgaattgcc cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg
    33301 cgattttaca aattggtgcg cttgaaatga tcaatcaaga accaaaacta aaagaaccat
    33361 tagaaaattt aagtgccacc gttcacactg ggatggatcg gattcgtcaa agtgtccatg
    33421 acctccatga gacatcgatc tcttttcaac actccttaga gtttctgatc gctgattttc
    33481 cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact
    33541 gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat caaacatagt catgccaaaa
    33601 ccgttgtttt acattttcga acgttgccgg ccttttatcg cttgcaaatc acgaatgatg
    33661 gcaccagccc acaaaaaaat tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg
    33721 ttcaagaaca aaagggacaa gtccatattt tccaagaaaa ccagcaattt cagttaacga
    33781 tcattttacc aagggaggga caccaatgat aacagtagtc attattgaga tgatccattt
    33841 gtgacgaaat cgttacaaac cattttagaa agcacgaaag agatccgtgt gttaggcatt
    33901 ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc atcgtcccga tgttttgctg
    33961 acggacatcc gcatgcctaa acaaacgggc atcgatgctg ccaaagaaat tttggctaaa
    34021 tttcccaaag ccatcctttt gcttttgacg acattcaaag atgaagaata catccgcgaa
    34081 gcattttcca tcggtgtcaa agggtattta atcaaacaga acttgcaagc gattattcct
    34141 tctgtcaaat ccgcttataa tggacaggcc gtatttggta atgaaatcat cgagactttc
    34201 actcaaatga tgaagaataa accgacgatt gatagtcatg cttatgcttc ttttagtgaa
    34261 cgtgagcttg caatcatcaa agaagtagct gccgggaaaa acaataaaga aattgcagat
    34321 gccttgtact tgagtgacgg cactgttcgc aattacatca gccaattact agaaaagcta
    34381 gatttacggg atcgcacgca attggctatc tatttttatc agcatttgca ataagtctta
    34441 tctaacacaa aaaaacctga atgatgctcc ttcgcatcat tcaggttttt ttcacatctt
    34501 ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa gaatcaagga ttcgcagggc
    34561 tgccaggatt cgttggattg ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg
    34621 ttttttctag ttttgattct tcacgatcgt ctcgttctac atcaggactt tcattcaatt
    34681 tgaattcttt ttccatctca tctaactttt taaagtcatt gccagattct tttgctaatt
    34741 tcgctaattt atcttcggtt tctttagcca taaagtaacg cccccctttt gatttcagct
    34801 tagcatatca cactgaaaaa acaaaagaaa acgctaacca acgatcattt ttagctattt
    34861 ctcatcttta aaatcaagca ttttttgccg gtataaaatc gtagggagaa ctgttttcca
    34921 taccaatcat cggatctact aactggttat cgatcacttc tgctgtaaga acgaaatctt
    34981 tagaaacagc aggttctacc tcttctttct tttcttctcc aacaaatgaa gaaaacagat
    35041 ctggttccac tgtttccatt acttggtttt tcgtttcatt cgtcccactg tcatcttcta
    35101 caggtagctt ttcttttaca acccgtggct cttttgtata gttcccatct tcaaatggat
    35161 catttccgac caaagaatct tcataagctg ctagtttggt ccggagagtg atattcattt
    35221 tatcattatc catgatcctt gatttcaacg tagtcatccg gcttgctgat tcattcgcta
    35281 cacaagtgac ataggcttgc tcttccccta acaaaatcaa cagttcctta ctgcgagtca
    35341 ctgccgtgta gagcaaatta cgctgaagca tccgctgata ttgatggacc atgggcaaaa
    35401 taaccatctt gaattcactg ccttgtgatt tatggattga gcaacaataa gaaagagtga
    35461 ttttattcca ttcgtttctt ttataagtga cctcatttgc atcaaactgg ataactaatt
    35521 catccacttt atcttcagaa tcttttgcca agatgatacc cgtgatttct cccatatctc
    35581 cattgaatac attcaattcg ggagtattga ctaattggag gactttgtct cctatccgat
    35641 aaacggtgtc gttccaatgt acttcctttt ttccaggttt ccctggattg aatatctcct
    35701 gcatcatttt atttaacgca tcgatccctg caggtccccg atacattggt gctaatactt
    35761 ggatatcttg tgcacggaat cccttatttt tcgctttttc cacaatttta gagatcaagg
    35821 gttcaatttg ataagcatca cttcggaaat aggaacgatc tttttgattc tttgtgaaat
    35881 cttttggcag acgtccttct ttgatctcat gggcaagagg aataatacta gaaccatcac
    35941 cttgccgata aatctgattg agttccattt tgggaatttg ttcgatttcc aaaagatcat
    36001 ggagtacttg accaggaccg acagagggaa gctgatcttt gtctcctact aagataactt
    36061 gcatattcgt cggaatcgct ttgaataatg tgttagccag ccatgtatcg accatagaca
    36121 tctcatcaac aatcaaaagt ccgccttcta attctttcgt actgactgaa ggtgtttttt
    36181 cccgtccatt cagccccaga agacgatgga tcgtactggc aggcagacct gtcgtttcgt
    36241 tcattcgttt agctgctcgg ccagttggtg ctgcaagtag aataggaaaa atttcttggg
    36301 tatattttga aggatccaat tccagtccgt tcaactctgc aaaaagattg acgattccgt
    36361 tgatcacagt agttttcccc gtacctggtc ctcctgttaa aataaataga ggggaacgga
    36421 ttgcttcagc gatagcttgt tcttgcgaag gtccatactg tatgccgaat attttttcga
    36481 tacgccgaat attcttctgc acttcttttt caggataatg gatttctttt ttacgactga
    36541 gaaggcgctg gatcgaatta ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa
    36601 gattagtctc ttcttgttgt atctttcctt cttcaacaag ttggataatt acagtagcaa
    36661 cctgatcagg ctggatttca attgggcgac ttgtttctaa aatacggatc gtttgctgaa
    36721 gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac tgcatgctga aaaatctgat
    36781 gcaagatagc tgctcgaatt cttctcggtg aatctgctgc aataccgatt tgttcggcta
    36841 gattatctgc acgtttgaac ccgattcctt ctatgtcttc gactagctgg tacggatttt
    36901 cttcgatgat atccaaagac tcgttcttat aggcctggta gatactgaaa gctaactgac
    36961 taccgaaccc gtaacgattc agtcccatga tcacttgatc catcccgtgg tttagccgaa
    37021 tcgtctcagc aagcatttct ctttttggct tcgtcaaacc ggagatttgt tttaataccg
    37081 aaggatcagc aatgattttt tcgatagcat cttctcccag caattctaca attttttctg
    37141 ctgttttctt cccgattcct gggaattttt cgctggaaag atacgctacg atcccatttg
    37201 cagttgttgg cttttcttgc tcatatcttt cgagtttaag ctgccttccg tatttaggat
    37261 gatccactaa ttctccataa aaacgataag tctcattttc ttgaatatcg ccgaaacttc
    37321 cggtgactac aatctctttt tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa
    37381 gcactttgta aaaattacta ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat
    37441 aaggcatttc ttctggctgc atcggaagcc gcctccttct tctatagtaa aggttctggt
    37501 gctgtttcag ttcctaaaaa atctgtttgg ttccatactt tcagttcata tggcaataat
    37561 ctgcaaggct cttcagtctc taaaatcgtc aaactactat tgaacaatcc acccatttca
    37621 cgcaactcac ttagctcttt tcctgtcatc cattgaatgg ctgcagttaa agaagctcca
    37681 tgtcctacga ataaaagtgg gccatcttta tgttgggcaa ccgcatctgc tacaacagtt
    37741 tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga
    37801 tctaaatgat tacgcaaatt cggcagatta tccggatatt tctggtacat ttcatcgatc
    37861 gactgacctt ctaaagtacc cagccctagt tctcttagct tatctgtata aaaaatctct
    37921 accggacgag caagctcttt gttgatacct tctgctgtat cacgtgctcg ctttgctgta
    37981 ctggaataaa ttgctttaaa aggaacatat tttacctttt tcccaaatgc tttgatttct
    38041 tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc cttgaaaacg tctttcctga
    38101 ttccattgtg tcttcccatg tcgggtaaaa tatagttcca tacgcagcaa actcctgtct
    38161 ttcttttatt ttactctata ataaactgtc taatccagct tgtcattttc atagtgatgc
    38221 gtcaattcta aattagggaa tccttgctgg cgaagtgctt catacactat cattgcagct
    38281 gtattcgata aattcaatga gcggacatgc tcatcattca tagggatgcg taaacacttt
    38341 tcttcgtttt cacgcataaa tgtttctggc aaaccggttg tttcttttcc aaataaaaag
    38401 aagtgctctt ctccatcagc aaaattttcg tcactatata ctttatgggc aaattttgag
    38461 atcaaatgta gtttacgatt acctaaaaat gttaaaaaag cttctaaatt actgtggtaa
    38521 gtaatattta catcattcca ataatccaac cctgcccgtt ttagatgttt gtcatcagtt
    38581 gaaaaaccta gtggctcgat taaatgaagc ggagtattcg tggctgcaca cgtacgggca
    38641 atatttccag tgtttgcagg gattaatggt tcaaataaaa cgatatgatt catcaggatc
    38701 attcctctca tatttatgtt cgtttctatt ttctgacaat ttgatttttg cataaaaaaa
    38761 aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt tagtgaagct cttctcacta
    38821 acttttatca gaacaggaac ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata
    38881 acaccctcaa cttttttttg caacctctac tcattaaaaa ctgctagaaa aacgttttct
    38941 cttagttcct tttaataatt ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa
    39001 gtctgctttg attcgctctt ctacctgcca ttctaaaggt gacatggcca aaagatcttt
    39061 ttgattttcc tctggtatct caaattcata ggttacacgc tgacgttcag ttaatttcat
    39121 tgcttcagca aacttggcta ctacacgttc attggaataa ctctgttttt caggttgatt
    39181 cggataaaaa gcctgacgta gttcctttaa ataattttct tgtggcacca ccttcaacac
    39241 ttctccatct tctgagagta cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt
    39301 taaaaccgcg ttgaatgttt gatcagcaaa cggcaaattg gtcaaatcag ctacacacca
    39361 aaatgcttcg gcagattgtt ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc
    39421 aacagcattt tgaatctttt tcaattttga aagttcattc aagaagctac cttctccgca
    39481 gccaacatct aatagcgaga cttcttcagg taaatgctgc gcaatagtct caataacggg
    39541 ctgatacatg ccagattgga tcattctacg ccttggaacg aacattcctt gatcgtattc
    39601 tgttttaacc gcatgggata aaaaataaag tgttcctttt ttggaaaggt caaaacggtg
    39661 attatttttg catatcaagc cattatcaga cttttttact tcttcttggc aaaccggaca
    39721 gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat
    39781 agttccccct catttctgtt ttatcttacc gttagatgct cactttggca actgctaaaa
    39841 atatgataca atgcctagaa gaaagtgaaa gataaaggag aagattagat gatcaaagaa
    39901 ttttgcgctg aaaactacac atctattcct ctagcaattg ctagaggagc cgatcggatc
    39961 gaattgtgtg acaacttagc ggtcggtggc acaaccccga gtactggtgt gatcgaagaa
    40021 acgttaagtt atgctgggga aaaagatatc cccgtaatga cgattattcg tcctagaggg
    40081 ggcgacttcg tctataacga tatcgaattg aaaatcatgc atactgattt gattgaagca
    40141 aagaaattag ggacggatgg cgtagtattt ggttgtctta ctccttcagg ctggctagac
    40201 gaagaggcct tagaactatt gatcgaaaca gcagaagggt tgcagatcac cttccatatg
    40261 gcttttgaca gtattcctga agatcggcaa ttcgaagcaa tcgattggct ggtagaacat
    40321 ggtgttgacc gtatcttgac tcatggcggt cctgctgaca aaactattgt agaaaatttc
    40381 ccacggctta aagaattgat tgcctataca gatggccgct taatcatttt gcctggtggt
    40441 ggtgtcactg cagagaatgc agcacatgta gctaaagaat tgaacgtaag cgaagtacac
    40501 ggaaccaaaa tcgtggatct tcagttataa tcttataaac aaaaaaacat ttgcaacagg
    40561 cataatgctt attgcaaatg tttttgtttt tttacataaa atagcccata aataaagaag
    40621 caatattgaa ataaatcagt acactcgtca aatcgctcaa agtcgtgata aagggaccgc
    40681 ttgctactgc tggatcaaat cctagacgat ccattagcat cgggattaaa ctacctgcaa
    40741 gattcgccac tgtgattgca aatagcatag ccatccctat gacaaacccc aagataaaat
    40801 tatgtttcca aaaacctacg attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa
    40861 cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa
    40921 tcgcaagtcg acgaacagcc acagctagcg actgggttcc agcattaccg gcagttcctg
    40981 taatcagcga aatgaagact gctaaaatac tcgcttcact taccaagtct tcatagtgac
    41041 tgattaaaga agcggtcgac atacctaaaa acaacagagt gatcagccaa ggcaaacgtc
    41101 gagacgctgc ctttactgga ttttcattaa tttcttccac attgaccccg gcaagtcctg
    41161 aataatcgct agcagcttca tcatcaataa cgtcgatgat atcatcgact gtgacgattc
    41221 ctaacaaatg atcatcataa tcggtaacag gaagagctaa gaagtcataa tctctaaatg
    41281 tttgtgccac atcttcttgg tcgtcgccta catgaactga aagcactcgt tcgctcatca
    41341 gatcagaaat cattgcgtcg tcatcattca cgattaggtc gcgcagagaa ataaccccta
    41401 ctagctgacc ttcttgatta acgacataga tgtaatagat tgtttcagct acatctgctt
    41461 cgttcttcag cacgtacatc gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt
    41521 cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg gagaagttcc ttgatttcac
    41581 tggcatcgtc tgccgacatc aaacttaaat atttagcaat ttgcttttta tccagcgtat
    41641 taagcaagtc aacagcatta tctgtataca tctcagaaag catatcagct gcatagctag
    41701 gacgcatttc atctaggtat tctttcatat gttcgttgtc ttcttcaatc acatcgaaca
    41761 tatccgctaa ttctttaggc gacagatagg aatagatcag ctgcctgttc tcttctgtaa
    41821 gagattggta gaattgcccc tgctcataga tatgcatctc taaaaaactg tttctgaatg
    41881 cctgaatatt attttccctt aaatcttgag tcaactgggc aaatctttcc tctaattctt
    41941 gattttcatc caaaccaatc tcacctcgcc ttattcttca aaccgtctca caactgcctc
    42001 catgtctgcg gctaaaggct gcttgaactc cagcaactct tctgtaaatg gatggtaaaa
    42061 acgcagatca taacaatgaa gggcttgtct ttttattccc agatccattc gcccgccata
    42121 catatcatca ccgagcaggg cacagccaat tgcggaaaaa tgaactctta tttgatgtgt
    42181 ccttcctgta tgcagctgga tatcaactaa cgcgatttct gttagtcgct tctctatcca
    42241 gtactcagtc tgtgccgaac gtccttcatc agagaccatt cgcttcaata gcgaagaata
    42301 atctcgggcg atcggcaaat cgatatgtcc atgtgccgat aaatctgaaa gccttccact
    42361 gactaaggcc tgatattttt tcacaaactt cttttcacga agttgcatat ctaattttgc
    42421 gtgcgcaaat ccatgctttg caaaaagcat caaaccagat gtatcccgat ctagtcgcgt
    42481 caccacatgg attacttggt tttcgtaacc ttgtttttta taataagcct ttacacgatt
    42541 agccattgtc ccatttggat ggtattgggc aggaatagac gaaacgcctg ctggtttatt
    42601 tactaccaga acgtgttcat cttcatacac gatatcgatc ggtgtttcat ccaaaagtac
    42661 ggtttcatgt tctccttcag caggaatcac aatggttacc ttatcatcct tagctagaga
    42721 aaacaagaca ttttctactt gatcattaac tttgatttga ccgccttgaa attttatttt
    42781 cgctaacaga ccctttgaga tccctttttc ttttaaaaaa tattttactt gctgagaatg
    42841 atctttctca taatcccatg taaactgcat atcaatcctc actaataaag gcatctttca
    42901 cacgatgcca aaaatgcata tgtcgataag aagcaaaatg gatccgttct tctgctattc
    42961 gataataaat tgagcggata tttgcttttg cgacattcaa ttgatcaacc gttacaagat
    43021 aatcttctgt attttccaat tttatttcta gccattcatc atgggcaatc acgatcggag
    43081 aacccaaagt acgaaacact cgattattca gtgaagcaat ctctgccaac tgaaaggcat
    43141 tgatactagg atggatcaca gcaccgccga cacttttatt atatgctgtc gaaccagtag
    43201 gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa cagctcgttt ttgatgtata
    43261 catcggctac catcgtacga ttggcccgtt taatcgttga ttcattcagt gctaaaaaat
    43321 gctggtccgg tgtttcatcc aaataactga tccgaacatc cagcaaggga taactaacac
    43381 tttgttcccg gttcgttcgt aagctgtcta ctagttcttc cagctcgtaa tcccgccaat
    43441 ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa acgcacatca ttcagacaat
    43501 gactgtagtg atggaacgcc gacaataatg tgccgtctcc gccgactgag atgaccaatt
    43561 ctggctcatg atcatctcgt tgaatccctg cttgttctaa aagtgcgttc aaacgttctg
    43621 tcacttctat agtctttgct tctttattat gaacgatcgc tactttcatc ccctgcctcc
    43681 tctctttcgc tttctttctt caaaagcgtt cgatagtaga catcatctga ttttcctctt
    43741 ccatgggaga ataaccgctg tgcttcttgg atttcttcac ggatactgga catctcttca
    43801 tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa
    43861 acgccttgat atttataatt cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga
    43921 gtccgaatct ggatttccgc caaaattttc ttctcaccgc tgatcagctg tacaggatac
    43981 tcgatcacca gatgataaga acggtagcca cttgcttttt tatttgtgat gtaatctcgt
    44041 tcttggagaa ctttcatgtc ttttctttta cggatgatct caacaacttg atggatatct
    44101 tctacaaact gacacatgat ccttaacccg gcaatatcag acatttcttc ttctaaacgt
    44161 cccattggaa tatgccgtaa ttgtgatttt gttacaatac tatcgactgg cttgactctc
    44221 ccagtgacaa attcaatcgg gacatgtcga ttttgttcac ggaactgttt gcggatcccc
    44281 cgcagtttca ctttcaattc tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa
    44341 tctcgttcca tagctgatac ctctacttca ttaacattca ttgtttattc taccatatat
    44401 gcaaagtttt ttcgtactct tgatttatgc attgaccaaa aaaagaaaat taggcaaaat
    44461 agacaaaaag gagtcgatca aatgacacaa atgcttgaaa tcgaatataa atctatgctg
    44521 acaaagcaag agtacgagat attcatcgct cactatcaat taacagataa agatttccgt
    44581 gtacagacta atatttattt cgataccgtt gatgaacagc tgaagaaaca aaattgtggc
    44641 ctacggatcc gtttcgttgg tcatcaagct gaatatacat taaaaacacc tgctgaaaaa
    44701 gggcgtctcg aaacaacgga tacctttata acagaagaag cagaagcatt tatcttcgaa
    44761 aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt ctgatttgaa tatcgatcct
    44821 gcttcattaa tccaaaccgg aaagcttaca acaaaacggg ctgaattccc tattgaagaa
    44881 ggacttttag ctattgatga aagctgggga gacaatctac acgattttga actggaacta
    44941 gaggtaaggg gatgcatctg tgggaaaaat tgcttttata aattttttaa aacgtttttc
    45001 tttaccttat cgtcctgcta agaacaagat ccaacgtatg cttgaagcta aaaattagaa
    45061 acctttcatt ttcacaaggc acttgagttc atttttttac ttttttctgt taagttatag
    45121 gatgtgaaag aatctagtca ctagttgtat agaatctatt ttgggggaga ttcatatgat
    45181 cgaaatctat ttatttgtta atcctttagg ggaatactgt ttcgaaatgg aacaacagtt
    45241 attacaattt atcgaagaag agtacgggac accatcaaaa gaaaagatgc aatttcgctt
    45301 tcttcctttg gtcaatttgc agacaattgg ggatgtcatg caacaaaaag gaatttctca
    45361 gaacaatctt gaggaacgta atcacttatt ctcaatgact tattctgcag cattggactg
    45421 caaagcggcg cagtttcaag gaaagaaaaa aggccgccgt tttttgatca aattgcaaga
    45481 agcagtcggc tgtaatgaca taccatattc tcaagaactt gctgaatcca tatttgaaga
    45541 aatcggcggc gatttgaata tgtttaaaga agatcgtcag tctgacttcg ttaaagaagt
    45601 attcttttct gatcaaaaaa ttgctcggga aatgggcatc acaaaacacc cttctgcagt
    45661 cgtttacaat tatgcctgcg accgtgattt tggtgtttta gtagaagtaa aaggtacaag
    45721 tagcctttgg agaatcacag agctgtgtaa aacaacagat aactcatatc aagtttttaa
    45781 tggtgaacat ttacatacaa atgattctca gttccattca agaactagcc atttacactt
    45841 gctttctaat taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt
    45901 ttctattcga gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt
    45961 catttagacg ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat
    46021 caactcctgc ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag
    46081 ccaagtagtt ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag
    46141 cagcagaaaa gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc
    46201 gtgaccattc gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat
    46261 tcaatttacc ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt
    46321 ccgtatgcat gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa
    46381 acccatccaa gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca
    46441 ataaatactc cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg
    46501 agtagtcgcc gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc
    46561 ccatttcgtg gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat
    46621 atggatttgt gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga
    46681 caacatcgat ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca
    46741 ttggtttcag tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag
    46801 cttcccctaa gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac
    46861 gtttccgtaa ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca
    46921 aagtatcgta tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc
    46981 tgtaattacg gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag
    47041 caaatgtatt tctaaattgt tcgtaaacac tgtacaatcc cttaaatgct gcttcgcgca
    47101 cccttcgatc cgtgctttct agcaactgac catacacgcc atgagataat tggactattt
    47161 caccattttc tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg
    47221 tatcacttga agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag
    47281 ataagacatg ggctcgatta tctactattt gctggatata atggcgatag acttccaatt
    47341 ttggttcttc tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt
    47401 caaaccagga aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg
    47461 cttggtaatc tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga
    47521 cttcagtttg gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc
    47581 cttgatcaag tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat
    47641 atttctcatc aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt
    47701 caggcaattc ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc
    47761 ccttagctct atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac
    47821 ttttctactc attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac
    47881 atagaaaacc gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt
    47941 atgcattcgt taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat
    48001 tcccttttat gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt
    48061 tccaaggctt cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa
    48121 aaccgacttt tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag
    48181 cattgttctt gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat
    48241 ttcttcccat cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta
    48301 ctagtgaata tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa
    48361 atatgccctg atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga
    48421 tcagctttcc agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc
    48481 aatgagaatt gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg
    48541 ttattcaaat aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg
    48601 atttctattg ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag
    48661 gcttccaaaa atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac
    48721 tgtaagtgct cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg
    48781 gcaaaatgag cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga
    48841 caataatatt tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc
    48901 attgcaagca gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga
    48961 aaaaagaggg cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata
    49021 gttcccaata tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat
    49081 ttatacgaaa aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt
    49141 tatacaaaat aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat
    49201 tcattcaatg tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc
    49261 tgattctcta catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag
    49321 cgctcttcga ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt
    49381 tggatcactt gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg
    49441 gcgcttccag ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca
    49501 ctcacatctt ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga
    49561 tttttactga taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct
    49621 tcgcttcctc ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt
    49681 tcgatttctt tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct
    49741 tcatgaccaa ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta
    49801 cacctcactt tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta
    49861 aagaaaaaaa atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc
    49921 aagctatctt atttaactat aacatttttg gcagacgaat actatatagc cgacttttca
    49981 gctaattttc cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc
    50041 taatcaagaa tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc
    50101 ttt
//
LOCUS       DADQRX010000011        42995 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.011, whole genome shotgun sequence.
ACCESSION   DADQRX010000011 DADQRX010000000
VERSION     DADQRX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.011"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            214..498
                     /locus_tag="J8V14_000605"
     CDS             214..498
                     /locus_tag="J8V14_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBA0920736.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="J8V14_000606"
     CDS             complement(779..1198)
                     /locus_tag="J8V14_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBA0920737.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="J8V14_000607"
     CDS             complement(1200..1784)
                     /locus_tag="J8V14_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBA0920738.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="J8V14_000608"
     CDS             complement(1831..3306)
                     /locus_tag="J8V14_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBA0920739.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="J8V14_000609"
     CDS             3487..3798
                     /locus_tag="J8V14_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920740.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="J8V14_000610"
     CDS             complement(3913..6249)
                     /locus_tag="J8V14_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0920741.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="J8V14_000611"
     CDS             6391..7059
                     /locus_tag="J8V14_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920742.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="J8V14_000612"
     CDS             7062..8105
                     /locus_tag="J8V14_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0920743.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="J8V14_000613"
     CDS             complement(8342..9223)
                     /locus_tag="J8V14_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0920744.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="J8V14_000614"
     CDS             9382..10110
                     /locus_tag="J8V14_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBA0920745.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="J8V14_000615"
     CDS             10266..10583
                     /locus_tag="J8V14_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA0920746.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="J8V14_000616"
     CDS             10576..11184
                     /locus_tag="J8V14_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBA0920747.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="J8V14_000617"
     CDS             11200..12030
                     /locus_tag="J8V14_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBA0920748.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="J8V14_000618"
     CDS             12168..12656
                     /locus_tag="J8V14_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBA0920749.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="J8V14_000619"
     CDS             12707..13579
                     /locus_tag="J8V14_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBA0920750.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="J8V14_000620"
     CDS             13755..14117
                     /locus_tag="J8V14_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBA0920751.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="J8V14_000621"
     CDS             14126..14878
                     /locus_tag="J8V14_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920752.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="J8V14_000622"
     CDS             14892..16694
                     /locus_tag="J8V14_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920753.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="J8V14_000623"
     CDS             16971..18011
                     /locus_tag="J8V14_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBA0920754.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="J8V14_000624"
     CDS             complement(18063..20207)
                     /locus_tag="J8V14_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0920755.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="J8V14_000625"
     CDS             complement(20564..21937)
                     /locus_tag="J8V14_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0920756.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="J8V14_000626"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="J8V14_000626"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBA0920757.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="J8V14_000627"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="J8V14_000627"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA0920758.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="J8V14_000628"
     CDS             complement(23385..24398)
                     /locus_tag="J8V14_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0920759.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="J8V14_000629"
     CDS             complement(24479..25078)
                     /locus_tag="J8V14_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA0920760.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="J8V14_000630"
     CDS             25264..25965
                     /locus_tag="J8V14_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920761.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="J8V14_000631"
     CDS             25975..28686
                     /locus_tag="J8V14_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBA0920762.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="J8V14_000632"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="J8V14_000632"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBA0920763.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="J8V14_000633"
     CDS             complement(30825..32006)
                     /locus_tag="J8V14_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA0920764.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="J8V14_000634"
     CDS             32258..33781
                     /locus_tag="J8V14_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBA0920765.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="J8V14_000635"
     CDS             complement(33850..34578)
                     /locus_tag="J8V14_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920766.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="J8V14_000636"
     CDS             complement(34591..35871)
                     /locus_tag="J8V14_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBA0920767.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="J8V14_000637"
     CDS             complement(36012..36614)
                     /locus_tag="J8V14_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HBA0920768.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="J8V14_000638"
     CDS             36859..37065
                     /locus_tag="J8V14_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920769.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="J8V14_000639"
     CDS             37377..38096
                     /locus_tag="J8V14_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBA0920770.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="J8V14_000640"
     CDS             complement(38177..38860)
                     /locus_tag="J8V14_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBA0920771.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="J8V14_000641"
     CDS             39078..41264
                     /locus_tag="J8V14_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBA0920772.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41610..42020
                     /locus_tag="J8V14_000642"
     CDS             41610..42020
                     /locus_tag="J8V14_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBA0920773.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            42034..42390
                     /locus_tag="J8V14_000643"
     CDS             42034..42390
                     /locus_tag="J8V14_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBA0920774.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        14022 a         7563 c         8595 g        12815 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag tgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataaa ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgtga caagaatttt gtcacacctc ccttatttat tttgaaaatg agaatactat
    41461 tatcaaaata gagaatatat agaaaaattc tcattattca aacgtaaata atcagttatt
    41521 ttgatttttt ttatatttta ttagctaata attggtattt taaaagaaat tactgtagaa
    41581 ttggattata attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta
    41641 cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga
    41701 atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag
    41761 gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt
    41821 ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga
    41881 gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc
    41941 ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca
    42001 ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg
    42061 cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa
    42121 tttgttgact tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt
    42181 gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg
    42241 attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat
    42301 ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag
    42361 ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc
    42421 atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt
    42481 gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg
    42541 tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct
    42601 gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg
    42661 acatgttaga aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc
    42721 ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg
    42781 ataaatgagt tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt
    42841 gacaattact tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg
    42901 gggaacactc ctgatgaagt agacctttga aaactgaaca aagtaagaca aaccaaatgt
    42961 gtagggcgtc ttgattcaat tcaagacaac aaaca
//
LOCUS       DADQRX010000012        41057 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.012, whole genome shotgun sequence.
ACCESSION   DADQRX010000012 DADQRX010000000
VERSION     DADQRX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.012"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..1125
                     /locus_tag="J8V14_000644"
     CDS             79..1125
                     /locus_tag="J8V14_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBA0920775.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="J8V14_000645"
     CDS             1474..2106
                     /locus_tag="J8V14_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBA0920776.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="J8V14_000646"
     CDS             2694..4133
                     /locus_tag="J8V14_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0920777.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="J8V14_000647"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="J8V14_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="J8V14_000648"
     CDS             4895..5110
                     /locus_tag="J8V14_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920778.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="J8V14_000649"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="J8V14_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="J8V14_000650"
     CDS             complement(5770..6681)
                     /locus_tag="J8V14_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0920779.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="J8V14_000651"
     CDS             complement(6700..7704)
                     /locus_tag="J8V14_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HBA0920780.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="J8V14_000652"
     CDS             complement(7701..9824)
                     /locus_tag="J8V14_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA0920781.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="J8V14_000653"
     CDS             complement(9829..12276)
                     /locus_tag="J8V14_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBA0920782.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="J8V14_000654"
     CDS             complement(12263..12652)
                     /locus_tag="J8V14_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0920783.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="J8V14_000655"
     CDS             complement(12712..13215)
                     /locus_tag="J8V14_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBA0920784.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="J8V14_000656"
     CDS             complement(13228..13452)
                     /locus_tag="J8V14_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920785.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="J8V14_000657"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="J8V14_000657"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBA0920786.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16331)
                     /locus_tag="J8V14_000658"
     CDS             complement(16212..16331)
                     /locus_tag="J8V14_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBA0920787.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16346..17530)
                     /locus_tag="J8V14_000659"
     CDS             complement(16346..17530)
                     /locus_tag="J8V14_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBA0920788.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="J8V14_000660"
     CDS             complement(17789..18079)
                     /locus_tag="J8V14_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920789.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="J8V14_000661"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="J8V14_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19444..20118)
                     /locus_tag="J8V14_000662"
     CDS             complement(19444..20118)
                     /locus_tag="J8V14_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0920790.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="J8V14_000663"
     CDS             complement(20196..21536)
                     /locus_tag="J8V14_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBA0920791.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="J8V14_000664"
     CDS             complement(21619..21954)
                     /locus_tag="J8V14_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBA0920792.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="J8V14_000665"
     CDS             complement(21964..22338)
                     /locus_tag="J8V14_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBA0920793.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="J8V14_000666"
     CDS             complement(22351..22665)
                     /locus_tag="J8V14_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBA0920794.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="J8V14_000667"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="J8V14_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBA0920795.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            26793..27116
                     /locus_tag="J8V14_000668"
     CDS             26793..27116
                     /locus_tag="J8V14_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0920796.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27280..28197
                     /locus_tag="J8V14_000669"
     CDS             27280..28197
                     /locus_tag="J8V14_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0920797.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28190..28972
                     /locus_tag="J8V14_000670"
     CDS             28190..28972
                     /locus_tag="J8V14_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0920798.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            28973..29653
                     /locus_tag="J8V14_000671"
     CDS             28973..29653
                     /locus_tag="J8V14_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0920799.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            29646..30542
                     /locus_tag="J8V14_000672"
     CDS             29646..30542
                     /locus_tag="J8V14_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0920800.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            30700..32676
                     /locus_tag="J8V14_000673"
     CDS             30700..32676
                     /locus_tag="J8V14_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBA0920801.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            32677..33123
                     /locus_tag="J8V14_000674"
     CDS             32677..33123
                     /locus_tag="J8V14_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA0920802.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            33139..33453
                     /locus_tag="J8V14_000675"
     CDS             33139..33453
                     /locus_tag="J8V14_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBA0920803.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            33479..34528
                     /locus_tag="J8V14_000676"
     CDS             33479..34528
                     /locus_tag="J8V14_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA0920804.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            34540..37143
                     /locus_tag="J8V14_000677"
     CDS             34540..37143
                     /locus_tag="J8V14_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBA0920805.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            37260..38909
                     /locus_tag="J8V14_000678"
     CDS             37260..38909
                     /locus_tag="J8V14_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBA0920806.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            39225..40160
                     /locus_tag="J8V14_000679"
     CDS             39225..40160
                     /locus_tag="J8V14_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920807.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            40153..40923
                     /locus_tag="J8V14_000680"
     CDS             40153..40923
                     /locus_tag="J8V14_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920808.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT        12498 a         7849 c         7682 g        13028 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aacagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacagg ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat
    26101 agaatggatt tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc
    26161 tttaaattag aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat
    26221 atatttacgg gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga
    26281 aaaaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
    26341 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
    26401 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
    26461 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
    26521 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
    26581 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
    26641 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
    26701 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
    26761 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
    26821 ggcttcagcg agaagttcca acgcatatac aaggacgact tatgtcaacg ataacaacga
    26881 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggaaga
    26941 caacgcaacc acgtctgcga gacagtctaa tttttctggg agggagtctt gctatagtta
    27001 tccttataac cggtctggta aaattactca agcttgatct gaaagaagca aaaattttca
    27061 gtgaagagga atacagagaa acgactttgg tcgaaaagaa agaatcagga aactgataca
    27121 agcgtataac aaactgcctg ataataggaa agacacgtat tgtccgattg ttttttttga
    27181 cattcaattg gaaaatttac actttcttta tacatttctg gctgctttct ttatacccgt
    27241 caactatact tcactcatca ataagaaaga aggataagta tggattttgg aaatgaaacg
    27301 gtcttgaaag caactggtat aaccaaaaaa tacggtgctg ccaaagcatt agataaagta
    27361 tcaatcgaaa tcaaacgtgg aatgatctat ggattgattg gggaaaatgg agcgggtaag
    27421 tctaccttta tgagaactat tatgggtttg atctcgattg atgaaggttc gatcgaatta
    27481 tttggaacga cggatttgca ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct
    27541 gctctttatc cagaattaac tgcaagagat aacctacgta tccaagcagc gaacggcggt
    27601 gtgtcggata gagaaatcga agacctgctg aaaatgatgc gattggaaaa tacagggaaa
    27661 aagaaagcaa agaatttctc tttaggtatg cgccagcgtt tagctattgc aaatgcactg
    27721 atcacgaatc cagaattttt gattttggat gaaccgacga atgggatgga tccagcaggg
    27781 atggcagaaa tgcgagaaat catccaacgt ttggtaaaag aacggggcat caccgtcttg
    27841 ctttctagcc acttgctaga tgaattgtca cagatcgcca ctcactatgg tattttgcat
    27901 gaaggacact tgatcaaaga attatcgaaa gaagaattgg cacaagaatc tcgtcaattt
    27961 ataaaaattg acacttcggc gacggaacaa gcagtaacgg ttctcgactc tcttggttat
    28021 agggattatt ttgtacaatc tagccgtgtt atccagcttt ttgaaggaat tgaccaagta
    28081 gcagccatca atcaagcatt ggtagaagca aaagttcctg tggatggtat tcacctagtt
    28141 gggcaaaaac tggaagatta tttcttacaa ttaactggag gcaatccaca tgtttaatct
    28201 attaactgct gaaaaaataa aagtatggaa gagccgtaaa atgtgggtct cactaggact
    28261 gatggtcgta cttccgcttt tttattcttg gaacgaatgg tatatgcata caaaatacgg
    28321 caatgaatta aaccaagcaa cggatacagt catcaacggt gcaacaggga ttttaatggt
    28381 agaaaaaatg gctgcatgga ttcttctggc tttcgcagca tttgcgtgct tttatattgg
    28441 ggaagaattt caaaacggta cgattcgcaa tacgttatct ttaggacgga atcgaatgac
    28501 ttattatgtg tcgaaacttc tcgttacgct tttgatcaca acacttggcg tggttttgat
    28561 cacgggacta gccatgcttg cttacacact tgctttcgga tttggtgaag ttgagggaat
    28621 caaaaactat ggaaattatg ttctaaaagt atttcctgta ttgcttctgc tgacattagg
    28681 gacattatct gttccagtgg ctttgacatt catcactaga agtaccagtg tcagtttgct
    28741 tctttctttt ctttatatca tgggaacggc cttcattccc ggtgttttcg caaaaatcaa
    28801 aggcttggaa tttctaacgg agtggtttac ggaaacgtgg ctcatgtata cggactttgc
    28861 gcaacaagca acttattcgc aagcaccgaa aatgattctg gtcagtctag taacaatcgt
    28921 ggtatcatca gctctaggaa tgtttatttt ccaaaaatct gacattaagt aggtggtcct
    28981 gctggaaact attttgatta tcgaagacga tgaagctgtt cattccttac tggaggaggt
    29041 tcttgaacag agatacaaac ttcttgatgc ttattcaggt acggaaggaa aattattgtt
    29101 aagcacatat cctgtcgatt tgatccttct agatctcatg cttccagggc tttcaggtga
    29161 agcattgtta gcagaaatcc gacagacatc gaatgttccg atcatcgttc tttcagcaaa
    29221 aagcgatcaa agagataaag tttccttgtt ggcagcagga gcggatgact atgtgacgaa
    29281 accatttgat atcgaagaac ttttgttacg aatcggcatc caacttcgtc atcagacttc
    29341 tgtccaagta aaagatctgt cgcaacgcat ctattacaag gaaatcttac tggacaaaga
    29401 gtcgagggat gtaaatgtga atggtgtggc tgtccatttg acaggaagag aattcgatct
    29461 gctgaagctt tttttagaac atccgaaaaa agtattttcg cgagcaaatc tttatgaaac
    29521 agtttggcaa gaaccatatt ttgatagtga gaaaacagtg aatatgcata tttccaatct
    29581 tcgagggaag ctagcaaagg cacatgctgt gtatattcat acggtgtggg gagtggggtt
    29641 tagatttgat taaatggctg ttgcttctcg ttgttatctt tatgggaagt tttattttct
    29701 tcctgcaaag ggaaatcaag agattaacta agaaaatcgc cactctttct gaacgacaag
    29761 aatatggtgg tcgtctttca gtcgaatttc gagaaaagaa tctaatggac ctagtagatg
    29821 cactgaatac atggttggat gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag
    29881 aaaacatcag actatcgatc gtcggccttt ctcatgatct cagaactcct ttgactgcta
    29941 ttaacggtta tgtacaatta ctggaacagg tagaggatac ggagaaacgg gagaagtatt
    30001 taacgatcat caaacaatct gttgcaagat tactcagtat gacggatcag ttttatgatc
    30061 ttgctcgaat agagacgaag caaaaagaaa tggatctcca tacgctttct ttttctaaag
    30121 aggtggaaga agtatttttc tctttttatg aacaattttc tgagaaaaat atcgaggtag
    30181 cgttcgctga acactcgata aatacaactg tattagcaga tccgctcatg ttgctgcgag
    30241 tgctgcaaaa tatcatacag aatttattgc gttatgcaga tggacaagca aaggtgacat
    30301 tttcaaaaga ggatccattc attcgattaa cagtggccaa tcctatcaaa ccaggaacgc
    30361 aaacctctgt tgaaaaagtc ttccagcgtt tttataccga aaataccagc cgcacaaata
    30421 cggaatctag cggaatcggg ctttacctgt caaaaaaatt ggtggaagga atgggaggag
    30481 agatgaccgc tagattagac gggggcatct tctccatctc cgtcaagttg cggagggttt
    30541 aattatacaa gattagtgag aactagtaga aaaatgtatt atcttatata tatttaatag
    30601 agaaaaaagc ctgtgatata aatattgcag gcttttttat tgatttgaaa taatcaagaa
    30661 tattagaaac ttctacatta atcaagaact ttcttctgta tgaaaataga gataataatt
    30721 gtacacttaa gctgtattca ggaggtgaag ataatgggca tcgaatttag cgaacggcaa
    30781 atgaaaatac tatacttttt gattgaaaat gagcacacaa cttacaaaga attatcagaa
    30841 caattatatg tatctacgaa aacaattagc aaagatattt taattttgaa agaaataatc
    30901 caaaaaatgc cagatgtaca aatgttggtt caacctaaaa aaggcgtttc gcttattgga
    30961 aaaaaagaaa cacttcgaaa tttattatcc agtcataaaa tcaatatcgc agactctccg
    31021 gaggaccgtc taacttatgt gtttgctaaa ttattgaata ctaatgattc tctaaagatt
    31081 caaaatcttg ctgacgaatt atacgtgagc aggtcgacgg tagaaagcac aatgaaagat
    31141 gttagaaaaa gactaaacaa gcatgggatg tccttagttt cagatcgaaa tggcgtgcgc
    31201 atcaaagctg atgaaaaaag taaaagaagg ttgatgtcag aagttgtgaa ctattattgg
    31261 ggtggcttta cagcatctaa cagtaaaaca gatctcttag acttgaatat caatttcatt
    31321 ggtgaagcga ataatttctt aaataaggac atattgaaaa aagtagccga attgcttaat
    31381 cgatacatga ataagacaca attgaaattt acagattatg aatttcaaac tttagctatc
    31441 caccttgcta ttgcgataga aaggattgaa acaagcaatt tcttatcacg tagtaataat
    31501 tctgaagaag aaatctcaaa gaatacactt attttaatca aaatgattga aaaagcattt
    31561 tcaattattg tgccaataga tgaacaagct tatgttgaca ttcacattac ttcgattgaa
    31621 aaaaatgcta tgaatcacga cttagatcaa acgaagcttt cctatttaga tatgggtgtt
    31681 gagctacaag agttagtaag aattaaatta aatgaatttt atcctgatga agaattgatc
    31741 aataatctag tattacatct caatgcagca gttaaaagac tcaaattggg agtaaatatc
    31801 tataaccctt atacggacaa aatcaaatat tcttttaaaa ggtcatttat gataagtgta
    31861 gatttattag aggaaatcga agagagattc tgtatacatt ttaatgaaga tgaaatcgct
    31921 tacgtaacac tccatattca atcacttttg gatcgttaca aacctgacaa aacgaaagtg
    31981 atattagttt gtagtagcgg atatggaaca tcaaaattat tagagcaaag aataaataac
    32041 ggttttgcag atatggttga gatagaagat gtactaagca tcaatgaact acaagataga
    32101 aaagtcactg atgaacttgt tataagcacg attccgattg aaaatacaaa ttttcctgtt
    32161 attgttgtta gtcctttgat gttaggggca gatgtcagaa aagtaaaaca gtatctacag
    32221 ttaaatgaac aaacagaaga tgcttttcta aatttattag ataaacgatt ttgctttttt
    32281 acccaccaaa taaaggataa tcaagtgaac gttttaaata taattaccaa aaaattggta
    32341 aaagaaaatt acgcgaaaga agggatacta gagggagcaa tcaaaagaga aaaactatct
    32401 tcaacagcga tgaatgattt tgctatgccg catatcgaag tagattttat aaaaaagcca
    32461 gtaatctgtg tctacgttaa tccagaagga attgattggg atgggacact tgttcattct
    32521 gttttctttt ttgcacttaa tgaaaaagta aaacctgaat tgaataaaat ttatgaaaca
    32581 ttcaatgaaa tcgcttcgaa tcatgagtta ttagcaaatt taaaaacgag ttcgtcgttt
    32641 gaaaaattag tttatatatt gaaagaaggc ttataaatga tcagtgaaaa aatcaatata
    32701 aataatatag tgatagattt aaatgcatct gataaaaaaa gtgtagtcga aaaattatcg
    32761 aaattgttag agactaatgg gtttgtgtct gaccacaaac aatttcaaaa agacgtattt
    32821 gacagagaaa accatatgac cacaggaatt gggaaaaata tcgccattcc tcatggcaaa
    32881 agcctagcag taattgaatc aacagtggct gtagccaagt taaagcggtc tgttgaatgg
    32941 ggcgcattag atgatcaacc agttgatctc gtctttttgt tagcaataaa agacactgat
    33001 aaaggtacta atcatttacg tattttagca gaattatctg gaagattgat ggatgacgag
    33061 tttgttaaaa gaataaaaga ttcgagaaat aaaaaagaat tggtaaaagc tctatcattt
    33121 taaaaggagg aatttcatat gacaaaaata gtagcagtaa cagcatgtat cgcagggatt
    33181 gcccatactt atatggcaaa agctaattta gaaaagtttg cgaagaagaa agggttagat
    33241 attaaagtag aaacacaggg ggctatgggg atagaaaaca aactttcaat ttcggatgtt
    33301 tccgaggcag atgttgtcat atttgcagta gatacaaatg tttcagaacg aggtcgcttt
    33361 gacggtaaac cgattttaac agtagggacc tcagaggtag taaaagatgg tgaagagacg
    33421 attgatgaag caattgcatt aattaacaaa taaaaaatat atcgtggggg agttactaat
    33481 ggaattcaag aaacaaggaa gagaaattct aaatcatttc caaagtgggg tttcctatat
    33541 gatcccgctt gtcgtagcag cagggttact aacttctatt gcagtcattt ttggtggcac
    33601 aggtgtttgg gaccagacgg atacattctg gggagtcctg cgaatgattg gtcagacggg
    33661 attacagttt atcgtaccga tgatttcagc ctatatcgca tattcaattg ctgaccgtcc
    33721 cggtctagca ccagctttta tcactgggat gatgtgtcaa aatcttggta tgggatttat
    33781 tggaggaatg gttgctggat tagcttgtgg ctatttagct aatgcattga aaaaagtaaa
    33841 agtaccgatg caagttatta gtctaaaatc aattatgctg attccttttg ttactacatt
    33901 agttgttgga atcatattat ggtatgtact agctacgcct attcaagcaa tgacaacagg
    33961 actgactaat tggctagaag gcatgtcagg agcaaatgct gctttgatgt cggctatttt
    34021 aggaggaatg atggcgtttg atatgggagg acctatcaat aaaatcgcat atgcttttgg
    34081 ggttgcttct ttttcaagcg gaagctttgg acctagtaca gccatgttat taggaattgc
    34141 tattccgcca tttggaatgt ttttagcaac aattttaaac aaaaaacttt atgatgaatc
    34201 ggaacgagat aatgcaaaga cagctatgat tatgggcctt gtggggatta ctgaagggac
    34261 gatcccgttt gccgtgaaag atcctttacg agtaatacca agcatcgtgg tgggcactgc
    34321 agtagcatgt ggtctaaatg gattttttgg tgttactcaa gaaacaatgc tttcaacctt
    34381 tatggcaatt ccttttgtta cgaatatacc tttatattta gtatcaattg ttattggggg
    34441 agtggttaca gcgttaatgg tcaatgcact aaaatccatc aagtataaaa aagaacaaca
    34501 agtaaattca gtagaagaag gaaagtagga gaatctgata tggtaaatgc acatataatt
    34561 caacacacgc actgggacag agaatggtat tttacaaatg aagatgccat cgtattgtct
    34621 gatcaggtct ttactgaagt tctagatgaa ttggaaagga accctaaagt aaacttttgt
    34681 ttagatggac aaacttcaat cgtcgatgag tatttggaga tcaatccgca aaagcttaag
    34741 gttatccaac gactgatgaa aacaggacaa ctattcgtgg gaccgtggta tgcacaaaca
    34801 gatgccttat tagtggatgc tgaatcaatt ttaagaaact taatgattgg aatcagtgat
    34861 acgatcctta aatatggaga tccaatgatg gtagggtatt tgcctgacac gtttgggttc
    34921 aatgcgaatt tgcctacact tttgcatcaa gtagaaatcg ataatttcat gtgttggcga
    34981 ggattaaact ttgaaacgat ggttccatct ccttatttta tctggaaagg attgggagat
    35041 aaatttgttt atgccatgaa ttttccattt ggttatatga ctggaatgat gaccttagac
    35101 gcaattaaag atattcctga atttgcaaaa gaaaagttgg atgaagcagt tgaaatgctt
    35161 catatgcaag gaaaaaacaa agatatttta gtaccttcgg gtgtagatca aaagtcaatg
    35221 attttaaatt ttgatcaagt cgtagagaag ttgaataaat acagtaaatt ccacaatatt
    35281 atttcagatt atccttctta tgtagaagtt atcagaaaca aaaaagaatt acctgaatat
    35341 aaaggtgaac ttcgtcagcc ggtttactct cgtgttcatc gttctattgg ctcagtaaga
    35401 acaaaaatga aaatagaaaa ttttaaattg gaacaaaaaa ttctaaaacg agttgagccg
    35461 ctcatgctga ttgcagaaag aaatggtatc catatcagta aaggcctact tcgtcggtta
    35521 tggaaaaaga ttttgcaaaa ccaggctcat gatagtattg gtggctgtgt ttcagataat
    35581 gttgcggaag atattttcca tcgaatcaaa gaagctaatg agatagctga cggaatcgaa
    35641 aaccttattg taaaacggat ggctgatgca ttaaagctaa aatcgaatga agtccttgtt
    35701 ttcaacacaa cgactgagaa ttatagtgga tcgaaaatcg tgcatgtagt agctgattca
    35761 aagaaaatac acttcaaagg tagtccctat aatatcattg aaaaagaagt ttattttcca
    35821 gaacggaaaa atgcttatcg gatgattgct acaggtttcg aatattttga tgaaccagct
    35881 tattatgaac ttgacataaa aataaatgta aatgtgccag cactaggata cactgttgtt
    35941 gaatttgaga aaaataatga aaaattagaa acagcaatcg agatagacaa aactgagata
    36001 agtaacaata aatataaact ttcttttaaa gatgggcaat tgaatttgat agttggagat
    36061 agacaatatc ttgatttcgt tcatttgatc gattcagcaa atgatggcga tacgtatgat
    36121 tattcaccat tagaaggaga tacagaacta tctctaaaat tagaaacagc taaagtatat
    36181 aaagattctt tacaagaaac attagttgtt tacggaaaag ctcaacttcc taaagattta
    36241 aaagatcgtt tatctgaaaa acccgaaatg gaagaaatat cttatgaaat atcgttcagt
    36301 ctgggagaaa gtcaaatcgt tgaaggcaca ctcaaaattc ataatactgt ttactcgcat
    36361 cgtatgcgct tagcaattaa tgttttagat aaatctgaaa aatcaattgc acaaattcaa
    36421 aacggattta ttgaaaatac ccctgatgtc attcctccga attggtcaga aactatggta
    36481 gagaaacctg ttaatttaga aatattcgat aagagtgtct ctgtggcaga aaatgatcat
    36541 tattttacgt tttatgcaga tggtttaaaa gagtatgaac gtatagaaaa tcaattaatc
    36601 ataaccttat tttcaacaac aggggaactt ggaaaaccta atcttgcttg gcgacctggg
    36661 cgagcttcag gagatacgac aaatgaaggg catgtcatga tggaaacacc gctagctcaa
    36721 gagataggtg agtataaagt cacttttgga tttaatgtag aagaaggaag atttaatgag
    36781 ttcaaagtgg caaaacaggc agaaaaacgg ttagaacaat ctatttctta tcaaaagcag
    36841 aaactgaatg tatttattca caggttagat aacaagattt ggccattaca aaatgaactt
    36901 gatgttcctc gtaaattttc attgattgaa ttacctgaag acttaattgt atcatcgatt
    36961 tatccttctt attttcatga acaaggattt gttcttcgct tagctaatcc aacagaacaa
    37021 gaaaaaatag tacctgaggc tatcctttcc cttgggacag ttgtcaatgc actggagaat
    37081 aaacaagaat taacaacaat tcctccctat gattatttgt caattttgat aaatgagaga
    37141 taaatatgta atatacaaga aaaaaacacc ttttattttg aaaggtgttt ttttaccctc
    37201 ttttcaaata gcgcaacagt gctatactaa agctaacaaa taaaggaacg gagagggata
    37261 tgggtcaaag agaatttatc gttttaatta ttattgcagc tgttatttta ttagttgctt
    37321 tagacatttt ttcacgtttg aaattaaagg aaacagttcg tagcaaatgg gaaaaaattc
    37381 cttatcaacc gagatttgat aaagaagaaa gcttgaagga agcatggctc acggagaaga
    37441 agttccgttc atgggatagc gaaatcgatg atcttacttg gtatgacctg gatatgttcg
    37501 aagtattcga aggaatcaac agtacctatt ctagtgtagg ttcagaagcg ttgtatcaac
    37561 gtttacgcag ctttgatttt ggtgaagatc agcagttaga aaaactgatt gctttttatc
    37621 aggaaaatcc tcaactgaga gaaaaaatcc aatatcaatt cgctcgttta ggaaaaaaag
    37681 atcataattt tgcaaaacaa tatctagcgg atggaaaaag taaaaagatt ggacgcgtag
    37741 gtttatttat ttttcttggc ttgcttccgc tggtgggttt gggtttgctt cttttaggac
    37801 aagtactagg aatctatttg gcaatcggtg ggttagtatt caatacggtc tattatctag
    37861 tgaagaaaca aacactagaa acagagctga atagcatgag ttatttggtt tcaacgattt
    37921 cagccgccaa tcagctagca aaactgccga caccacttca agaagagctg aaacagcaaa
    37981 ctaaaccatt aagagacatc cctaaattag gtttttcatt tcgtatgaag agtgggagtg
    38041 aagcagagtt gatttttgaa tatgtcaaca tcatgttcat gctgccattt atctcctatc
    38101 atttcgttct cactaaatta gaaaagcatt caactgaagc catcaagctg tgggaattgc
    38161 tgggagaatt agaagtagcc gctgcaatat tgaattaccg tacttatatg cccatcactt
    38221 gccgaccaga gtttactgag ggcggtgtga cagcgaaaga tatctatcat ccattgttaa
    38281 aagaagcggt agtgaatccg gtcaactggc agaagaatac attagtcacg ggttccaatg
    38341 catcaggaaa atctacgtat gtcaaaagta tagccatcag ctgtattcta gcacagacga
    38401 tccaaacagc gattgcagaa cagtttaccc tgcaacctgg acatgtgttg acttccatgg
    38461 caatcgaaga tgatttattt gaaggcgaca gttattttgt ttcagaaatc aaatcgatca
    38521 aacgtttgtt ggaccaagtg gcttccaaag aacgctgcta ctgcttcgta gatgagattt
    38581 taaaagggac aaatacgatc gaacggattg cttcttccgc ttcagtcgtt cggtggcttg
    38641 cggagtatcc atctttagca tttgtcgcca cacatgatat cgaattgaca gagattttaa
    38701 agaattattg tgagaatgtc cactttgaag aacaagtaac agaaggaaaa ggaatcagtt
    38761 ttgatttcaa attaaaagaa ggaccttcaa ggacccgaaa tgcgattgcc ttgctgaaag
    38821 ttttggatta cccagagact ttagtagaaa ccgcaaaaac ggaagccgcg ttatttgatg
    38881 aaaagcggca gtggcatgta ttgggatagg cgaagtgatc gaaagtattt attggatcaa
    38941 aaatcttggc ttttgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
    39001 ttacttttgt ctcacgctcc ttttgttgca catatgaata gtaatccttt atcttcgatt
    39061 tgttttttct gaaaaggttg tctgacttcg tccgattgat gtctttcatt aggagatttt
    39121 cctgagccca gcaacctgat ttttcgctgg aaccaagttg ttttttcatc tgctgtattc
    39181 catgatacaa taaagaatca tgtctataag gagataggaa ataaatggca aatgcgcttg
    39241 aaataaataa tttgaaaaag gtttatggaa gtggggtcga agcattgcga ggaatcgatc
    39301 tttccgtgaa agaaggagat ttctatgcgc tattaggacc aaatggagct gggaagtcta
    39361 caacgatcgg aatcattact tctttagtca ataaaacatc tggaaaagtg aatgtcttcg
    39421 gttatgatct ggatacggat ctagtccgag caaagcaaca gattggatta gtcccgcaag
    39481 agttcaattt caatcccttt gaaacggtcc agcaaatcgt cgtgaatcaa gcaggctact
    39541 acggtgtctc tcgcaaagaa gcgatgaaac gcagcgaaaa atatttgaag cagtccaatc
    39601 tttgggaaaa gagaaatgtc cgtgcacgta tgctttccgg gggaatgaag cgaagactga
    39661 tgattgctag ggcattgatg catgaaccac gtttgctgat cttggatgaa ccaacagcag
    39721 gagtcgatat tgaattgaga cgcgagatgt gggagtttct ccgagaattg aacgaaaatg
    39781 gtacaacgat cattttgacg acgcattatt tagaagaagc tgaaatgctt tgtcgcaaca
    39841 tcgggatcat ccaatcaggt gaactgatcg agaatactag tatgaaagag cttctatcca
    39901 aactgcaatt tgaaacattt atctttgatt tagcgccata tgagaccaaa cctgtgattg
    39961 aaggatacgc ttgtacattt gaagatgaac agacaatcgc tgtcgaagtt gaacgaaatc
    40021 aaggggtgaa cggaatcttc gatcagctga caagacaagg aatcaaagtt ctttctatga
    40081 ggaataagtc gaatcgttta gaagaactgt ttttgaaaat cacagaagaa aataatcata
    40141 cggaggagaa aaatgtttaa tctttatttt actgcattaa aaagtttagc tatcaaggaa
    40201 acgaaccgct atttgcgtat ctgggtccaa acactggttc cgcctgtgat cacgacatcc
    40261 ttgtactttg ttattttcgg aaaaatgatc ggtggtcgga tcggtgacat gggtggtttt
    40321 tcatatatgg aattcatcgt gcctggtttg attatgatgt ctgctatcac tagttcgtat
    40381 gccaacgttt cttcgtcctt cttctctcaa aaattccaga aaaacatcga agaaatcttg
    40441 gtagcgcctg ttcctacgca tgtgatcatt tggggctttg tgattggcgg agtgggtcgc
    40501 agtattctcg tcggctctct cgtaacgatc atttccttgt tctttgtacc acttcatgtg
    40561 tattcatggt tcatggtcat cattacatta ttgatgacgg caatcttgtt ttcgttagca
    40621 ggactgctca acgggatttt tgctcagtct tatgacgatg tatcgatcgt gcctacattt
    40681 gtcttgcagc cgttaactta tctaggtggc gttttctatg ccatctctat gttgccgcca
    40741 ttttggcaag ctgtttcgaa agtcaatccg atcgtttata tgatctccgg tttccgttac
    40801 ggattccttg gaacaatcga tgtgccagta gcaacatcga tgatcgtact tgtgctattc
    40861 atcgttgttt tgtacgccgt tacttggtat ttgatcaata aaggaagagg attgagaagt
    40921 tagaaaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacgggaaaa
    40981 tagttcctca tattttgtcg tttgttgagc taataccgag ggtcagctga ttgaacaccg
    41041 tttagtggag aggttgt
//
LOCUS       DADQRX010000013        38205 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.013, whole genome shotgun sequence.
ACCESSION   DADQRX010000013 DADQRX010000000
VERSION     DADQRX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.013"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1218..1544
                     /locus_tag="J8V14_000681"
     CDS             1218..1544
                     /locus_tag="J8V14_000681"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA0920809.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1552..2460
                     /locus_tag="J8V14_000682"
     CDS             1552..2460
                     /locus_tag="J8V14_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA0920810.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3089..3340
                     /locus_tag="J8V14_000683"
     CDS             3089..3340
                     /locus_tag="J8V14_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBA0920811.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3882..4040
                     /locus_tag="J8V14_000684"
     CDS             3882..4040
                     /locus_tag="J8V14_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0920812.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4112..5337
                     /locus_tag="J8V14_000685"
                     /pseudo
     CDS             4112..5337
                     /locus_tag="J8V14_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(5426..5818)
                     /gene="rpsI"
                     /locus_tag="J8V14_000686"
     CDS             complement(5426..5818)
                     /gene="rpsI"
                     /locus_tag="J8V14_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBA0920813.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5834..6277)
                     /gene="rplM"
                     /locus_tag="J8V14_000687"
     CDS             complement(5834..6277)
                     /gene="rplM"
                     /locus_tag="J8V14_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBA0920814.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6796..7263)
                     /locus_tag="J8V14_000688"
     CDS             complement(6796..7263)
                     /locus_tag="J8V14_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBA0920815.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7383..8921)
                     /locus_tag="J8V14_000689"
     CDS             complement(7383..8921)
                     /locus_tag="J8V14_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBA0920816.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9001..9687
                     /locus_tag="J8V14_000690"
     CDS             9001..9687
                     /locus_tag="J8V14_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA0920817.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9888..10415)
                     /gene="msrA"
                     /locus_tag="J8V14_000691"
     CDS             complement(9888..10415)
                     /gene="msrA"
                     /locus_tag="J8V14_000691"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA0920818.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10499..11176)
                     /gene="pgmB"
                     /locus_tag="J8V14_000692"
     CDS             complement(10499..11176)
                     /gene="pgmB"
                     /locus_tag="J8V14_000692"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBA0920819.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11173..12301)
                     /locus_tag="J8V14_000693"
                     /pseudo
     CDS             complement(11173..12301)
                     /locus_tag="J8V14_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12301..14484)
                     /locus_tag="J8V14_000694"
     CDS             complement(12301..14484)
                     /locus_tag="J8V14_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBA0920820.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14481..15506)
                     /locus_tag="J8V14_000695"
     CDS             complement(14481..15506)
                     /locus_tag="J8V14_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0920821.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15551..16378)
                     /locus_tag="J8V14_000696"
     CDS             complement(15551..16378)
                     /locus_tag="J8V14_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA0920822.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16375..17442)
                     /locus_tag="J8V14_000697"
     CDS             complement(16375..17442)
                     /locus_tag="J8V14_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBA0920823.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17468..18304)
                     /locus_tag="J8V14_000698"
     CDS             complement(17468..18304)
                     /locus_tag="J8V14_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0920824.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18301..19170)
                     /locus_tag="J8V14_000699"
     CDS             complement(18301..19170)
                     /locus_tag="J8V14_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0920825.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19240..20523)
                     /locus_tag="J8V14_000700"
     CDS             complement(19240..20523)
                     /locus_tag="J8V14_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA0920826.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20537..22222)
                     /locus_tag="J8V14_000701"
     CDS             complement(20537..22222)
                     /locus_tag="J8V14_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBA0920827.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22497..23522
                     /locus_tag="J8V14_000702"
     CDS             22497..23522
                     /locus_tag="J8V14_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0920828.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24117..27770)
                     /gene="rpoC"
                     /locus_tag="J8V14_000703"
     CDS             complement(24117..27770)
                     /gene="rpoC"
                     /locus_tag="J8V14_000703"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBA0920829.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27849..31466)
                     /gene="rpoB"
                     /locus_tag="J8V14_000704"
     CDS             complement(27849..31466)
                     /gene="rpoB"
                     /locus_tag="J8V14_000704"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBA0920830.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32194..33162
                     /locus_tag="J8V14_000705"
     CDS             32194..33162
                     /locus_tag="J8V14_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0920831.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33180..34085
                     /locus_tag="J8V14_000706"
     CDS             33180..34085
                     /locus_tag="J8V14_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920832.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34082..34888
                     /locus_tag="J8V14_000707"
     CDS             34082..34888
                     /locus_tag="J8V14_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0920833.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35045..36013
                     /locus_tag="J8V14_000708"
     CDS             35045..36013
                     /locus_tag="J8V14_000708"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBA0920834.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36330..36854)
                     /locus_tag="J8V14_000709"
     CDS             complement(36330..36854)
                     /locus_tag="J8V14_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HBA0920835.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37141..>38205
                     /gene="scm"
                     /locus_tag="J8V14_000710"
     CDS             37141..>38205
                     /gene="scm"
                     /locus_tag="J8V14_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBA0920836.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        11012 a         8053 c         7100 g        12040 t
ORIGIN      
        1 gtcattcttt acaacgattt atagaaaaac aacctcttta aatataaaag gtacgggtta
       61 ctgattactt gtaaatcttt acgttccagt tttattcttt ctaaaaaata tactcacttc
      121 ttttcttaga cttctttttt tgattctcct ctttatggtc ttgtcctttt ttgccttcaa
      181 aatatttaaa taaacccctt tcaaaagcat ttctaggttt tggacaataa ggaatgactt
      241 ttttcttcat ataaatacct catttaacta tttgatatca tcacattatc aaaaaaaata
      301 ggttttttat taaaactatc ttgacctttc gggagatttc actgtgattt atttttattc
      361 ccatactttt ttcgttggtc actttcgtat acctaatcta agcgatcatc ggcgacggtg
      421 cttttacaac gaatggcagg acaaaacaag gggcggaaaa acttttttgc ctatttcata
      481 aaaaactttt tcctacagca atcccttgtt ttgtcctacc acacgttgtg aacccaaagc
      541 acgccgacga acgcttagct atacgaaaat gacctccaaa aacaaaaaag tgaggtgaat
      601 agaaacaaaa agaaattgaa atctcccaaa atgtcgtccg cctcggagac aacttgctgc
      661 tgaaaatggt tggttaattc atttaagtat taaataagat aaatcatcaa attaatcgga
      721 aaaatagcat gattggcctt atcaaaaata aactgtaaaa acttatattt ctatgagtgg
      781 tggaacaatg ctaataccaa attaatttat cgattaccca cctgcacaaa ccaaagatag
      841 cattattagg aaacgaatca atcagcgaat ccagcattat aatgaggata ctaaaaataa
      901 agttttcata attataaatc cgccttccta cgaaaggcac caatgaggtg atgaaaacct
      961 tagagcgctt tctttctttt tttattccgt gttacccttt ccttgcaggt ccctggtaca
     1021 ctacagaacc cgaggaggtg agtggcgggg ctgtatagcg gcaaccccgc ataattatat
     1081 ggtgtcggtc atccgttaag gcatcaatga atggcgcaaa cccgtagccc gtaaacttat
     1141 ctcttccgaa gttgactcga tttcgccgga tgaccagtcc ctaccaaacc tgtactattt
     1201 ttttacagaa ggttgctatg ttcaaaatct ttcgtaaaaa ttgttgtggc ttggatgtcc
     1261 acaaaacgtg gatctatgcg tgtatcggaa tcacagatgc gaatggtcgt acagtgtata
     1321 agcaagctcg cttctcttcc tttacaaaag gtctcaatga attagctgaa tggcttgcta
     1381 aatacaactg taaggatgtg tgtttggaat cttccggtaa attttggatt cctgttttta
     1441 atattcttga aaaaacttgt tttgtcacac tggctcatcc gaagtacacc aaaccccaga
     1501 aaggaaataa aacagatcgc aaagatgcaa aaatggattt gtgatctctt tatgtgcgac
     1561 atgatcaaac ccagttttat tcctggtctt gaaatccgcc agcttcgtga tctaatgcgt
     1621 tatcggacga aactgaccaa catgaaaaca agtgaaaaga atcgtgcttt aaactgctta
     1681 accgtttcta atctgaaact agatgatgtt tttagtgatg tgttcggaaa atcatccact
     1741 tctatcatcc aatacttgtt agaacatccc ggtgaatcat ttgatgttcg accatttatc
     1801 catgcccgtt gtaaaactcc cgttgaagaa atccaagcgg cagttgaggg ggcgatttct
     1861 gtacctcaag cgattaagct tcgccaatgc ctcaaccata ttaacgagat tgagaagcat
     1921 cgagaagaga tcaatctaga gattcaagct cttgcagaac ccttctcccc tgttttggaa
     1981 ctacttcgta ccataccggg cttagacact cagccaataa cagcgattgc gattctttca
     2041 gaaataggac cggatatgtc cgtgtttcca acttcgaaac actttatttc ttgggctgga
     2101 tgctgtccaa gaaacgatcg cagtgcccga cgaacaaaat caacacgtat ctctcgtgca
     2161 gggcagtcgt tgaaaccgct acttgtacag attgccaatg ctcttctcag atccaaagaa
     2221 catccagaat ttaaaaatcg ctatcgtaga atcaaagcga atcgtggaca caagaaagct
     2281 atcattgcta tttgtaagat gctcctgacc gctatctgga atgtcttatc aaaggtggaa
     2341 ccctattctg ctaaaggata tctggagcca aaacctgcga aagaagagaa agttctgacg
     2401 caatcccaag ctctggacct tttaagaaaa cgcggatata ccattataga tagtgcttaa
     2461 accaaagtta cgttgattct tcctaaatag actcgttgtc ttccatttca aaaattttga
     2521 aaacggtagg cttatttagt gtaccgttct tcaggtatct tcaaatagat ttattttcaa
     2581 acttatttcc tttcttcttt tactctccta tagcttagca tttaagtggg cactcgtgtc
     2641 accttgctag atagctatga atgccgtaat aagcctccta gtatataggc agactgccaa
     2701 ttataactga ttcaatccac attatgataa aatccacctt ttcgtttcat atgtgttgaa
     2761 atatcacata tgtcaaaagt gcacgagtct tttcattcag tgctttgtaa atactcaaaa
     2821 ttattccttt tcactttaaa aaaaagataa ttagtacaaa atcagaaatg aggaaagtat
     2881 taggatggac tagaaaggag ttgttgaatt tgttgcaaca gattggtcat ttatcaatta
     2941 aaaattaggt gaagttttat tcatcactgc agctgctgtt tcccagagaa taaaacgtct
     3001 agaagaaact ggtcagttac tgtattatca agcagcaatt gactacgaac aagcttgatt
     3061 actaaccaaa gcgtttatcc acctgttgat taaatcaaat caaaaatcag gtttttatac
     3121 gttcataaaa gcaatctcca gtgtactcga atgtgactgt atcataggtc cttattcgat
     3181 gttaatcaaa atggggtttc cttctaccga atcattggac agttttatca atgatttatc
     3241 aaagtttgga cgaaccaaca cccaaatcgt tttctcgact cttgtttcct taatggttta
     3301 tcagttctgg aatgtttatc atgcgctttt attaaaatag aaaagagctt ttttcatgcg
     3361 acaaagcagt gatcatttat tggcacatgc aatctttgtg gatccatttt ttgaaaatac
     3421 gatatttagt tttgacccaa aaatctttaa atacgtattt gataatttat cttaacaacc
     3481 agataatttt attcagcacc gtattatcta tattgaattc tcggaatcaa gataaatttt
     3541 tttccgtttt tgtttctttt ttaatcttct tcttgttatt aatagtgatt aggatgatat
     3601 tcctatattg atctttgata attagatata atttttgaat aacgttccct gctattcgag
     3661 ctagaaatac aagtacgcca tgacctagtt agcgacaact acttagagat ttcttttgtt
     3721 ctgggacaac aaaatgccat gacaatagca gtaaataatg atacttccgt aaggctcgcc
     3781 gtaaactggg gagaggtgaa attcctatgt gcttacccaa agcggttcaa aaattttaaa
     3841 ctattatttt aggagatgat tttataaaat cgaaagccta cttgaataaa aaacaagcct
     3901 gtgaatttct aggaattgat aataatactt tagataagtg gattcgtgta tataattttc
     3961 cagtaataaa aattgatggc gtagttcgct ttgacgtaga ggatatcaaa gatttcatga
     4021 atacattcaa aaaaaattga aattttcttc tcgtcacata tactataaat agcgaaatgt
     4081 actgccttac tttttaaagg agtgaggaag tttgcctaca aagctttcaa atgggaaata
     4141 taaaactaat ctgaggtatc caaaaaaatt cagagaaatc actggaattg catcagagaa
     4201 gtatcaaaaa gtatttccaa ctaggcaatt agctattaag gccgaaaggg aaatgaaaaa
     4261 gaagattgaa acagttttaa gagaggagaa tgcaaactca ctagaattga aaggaaaaat
     4321 aaaatttaaa gatttttatg aatcaaaatg gcttcctaga tatgaactag gtcaaactac
     4381 taggtcaaac agggctccaa gtaatgtaac aattagtaat actaaggata tattccgatt
     4441 gcacatcctt ccaatgtttg gtgaatatgc aatgaattat ctgaattcaa atactgagat
     4501 aatatcagat gaattaacaa agaaatcgaa agaatacgca aatataaaaa ttatcaaagg
     4561 ctatgttcgc tctatgtttg atattgccga aatactatgt tatatcgaat tcaaccgaac
     4621 taccaaaatt attcaatgta ttaccgcgcc taagaaaatg gctttagaag aaaaaaggat
     4681 aagggaaggg aatcaagcat tatcatcaga agagctgact gattggatag aagcagtaaa
     4741 caatgactta aataatcact tgcttacact tcacgattac acacttttca tgcttacctt
     4801 atatctaggt gatagaaaaa gtgagactta tgctctgcaa tggaaatata ttgattttaa
     4861 taaaaatacg gttcgtctca aatatgcttt agataaatat caaagaaaga cacatacaaa
     4921 aggttggaaa gacaccgtta ttcaggcacc agaagttgtg atgacattat taagagaatg
     4981 gaaatcagct caggctgaac aattgttgaa actaaaaatt atacaaacac ctgaccagta
     5041 cctctttacc tattccaaac cttctggaga agtaaattgt cctgttcacg ctgactactt
     5101 aaactatcga atcaatgcaa taaaaaaagg catcctgaat tggctcaatt atctccacac
     5161 aaacttcgtc atacctatgc aactatagct agggaaggtg gagcaaatat gaatcaaatc
     5221 tctaatgctt taacgcactc ggatatttct actacaaaaa tatacgtgaa cacacctgac
     5281 gtagttgata aatctgtttt tgaagcattt caaaaaggat tgaataagtg tgattaacat
     5341 gtgttctcaa atgtgttctt attataaaaa aagacacttt ccaaataatt gaaaagtgtc
     5401 ctgaaatatt gatataaaaa gaatattaac gttttgagaa ttgtgaagct ttacgagctt
     5461 ttttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
     5521 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
     5581 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
     5641 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
     5701 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
     5761 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
     5821 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
     5881 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
     5941 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
     6001 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
     6061 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
     6121 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
     6181 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
     6241 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
     6301 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
     6361 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
     6421 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
     6481 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
     6541 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
     6601 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
     6661 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
     6721 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
     6781 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
     6841 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
     6901 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
     6961 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
     7021 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
     7081 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
     7141 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
     7201 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
     7261 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
     7321 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
     7381 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
     7441 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
     7501 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
     7561 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
     7621 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
     7681 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     7741 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     7801 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     7861 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     7921 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     7981 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     8041 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     8101 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     8161 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     8221 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     8281 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     8341 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     8401 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     8461 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     8521 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     8581 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     8641 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     8701 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     8761 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     8821 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     8881 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     8941 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     9001 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     9061 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     9121 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     9181 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     9241 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     9301 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     9361 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     9421 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     9481 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     9541 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     9601 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     9661 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     9721 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     9781 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     9841 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     9901 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     9961 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
    10021 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
    10081 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
    10141 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
    10201 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
    10261 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
    10321 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
    10381 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
    10441 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
    10501 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
    10561 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
    10621 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
    10681 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
    10741 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
    10801 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
    10861 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
    10921 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
    10981 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
    11041 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
    11101 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
    11161 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
    11221 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
    11281 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
    11341 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
    11401 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
    11461 gaggtctgct tgctgtaccc gtatcgaact ccagtaaatc caatacgtag tcgatgccag
    11521 agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac
    11581 tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt
    11641 cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc
    11701 cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca
    11761 gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg
    11821 cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga
    11881 tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt
    11941 gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt
    12001 ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac
    12061 cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt
    12121 gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta
    12181 taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt
    12241 gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca
    12301 ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac
    12361 aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac
    12421 aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg
    12481 aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat
    12541 aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg
    12601 atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc
    12661 ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt
    12721 tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa
    12781 taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct
    12841 acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc
    12901 aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac
    12961 tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc
    13021 ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt
    13081 gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc
    13141 gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt
    13201 tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag
    13261 gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt
    13321 gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg
    13381 gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc
    13441 ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag
    13501 gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt
    13561 cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc
    13621 gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg
    13681 ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt
    13741 ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac
    13801 gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt
    13861 gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat
    13921 atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg
    13981 cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt
    14041 tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg
    14101 atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc
    14161 agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta
    14221 tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct
    14281 gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac
    14341 accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat
    14401 accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc
    14461 aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat
    14521 aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc
    14581 tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact
    14641 ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga
    14701 tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata
    14761 ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca
    14821 aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt
    14881 tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca
    14941 gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct
    15001 tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc
    15061 gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg
    15121 tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct
    15181 gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca
    15241 ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattggctgt acagacactc
    15301 acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat
    15361 cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat
    15421 tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca
    15481 gcgcctatga ttgctacttt cttcatttat ttctctctcc tttgcttatt tacttattgt
    15541 tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt
    15601 tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt
    15661 catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat
    15721 caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat
    15781 actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa
    15841 aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg
    15901 ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac
    15961 ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc
    16021 cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg
    16081 ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt
    16141 gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt
    16201 tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat
    16261 tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc
    16321 taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg
    16381 ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata
    16441 acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca
    16501 atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca
    16561 tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt
    16621 gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata
    16681 agaaatgatc ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc
    16741 actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc
    16801 gacatctgct ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct
    16861 acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg
    16921 aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac
    16981 atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt
    17041 ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag
    17101 cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa
    17161 actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac
    17221 cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc
    17281 ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt
    17341 agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt
    17401 gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct
    17461 tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata
    17521 acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc
    17581 aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg
    17641 aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa
    17701 cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga
    17761 tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat
    17821 aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg
    17881 ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca
    17941 aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga
    18001 agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg
    18061 ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat
    18121 tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg
    18181 caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat
    18241 tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt
    18301 tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa
    18361 tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa
    18421 ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa
    18481 catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt
    18541 cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt
    18601 tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca
    18661 gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc
    18721 ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat aactattatt
    18781 gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat
    18841 cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa
    18901 tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga
    18961 acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc
    19021 gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg
    19081 aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt
    19141 taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag
    19201 ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc
    19261 gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc
    19321 gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc
    19381 aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt
    19441 gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc
    19501 accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt
    19561 gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga
    19621 tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga
    19681 gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt
    19741 tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg
    19801 ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc
    19861 gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc
    19921 actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt
    19981 gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc
    20041 tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat
    20101 atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc
    20161 tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat
    20221 cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa
    20281 cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc
    20341 tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc
    20401 gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc
    20461 accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt
    20521 catttttttc cacctcttat tttattattt tcagccacat atattcatac ggctggaata
    20581 tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc
    20641 cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact aagactttct
    20701 cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca
    20761 aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac
    20821 catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa
    20881 atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc
    20941 gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg
    21001 aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg
    21061 catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt
    21121 agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg
    21181 gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa
    21241 aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat
    21301 tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg
    21361 cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag
    21421 tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt
    21481 tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag
    21541 cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt
    21601 tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc
    21661 atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg
    21721 gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg
    21781 tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga
    21841 caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca
    21901 aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga
    21961 aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct
    22021 taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga
    22081 aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac
    22141 tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg
    22201 cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat
    22261 ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata
    22321 cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat
    22381 ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg
    22441 tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga
    22501 aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag
    22561 tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca
    22621 aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg
    22681 cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag
    22741 atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa
    22801 cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga
    22861 tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt gaattggatc
    22921 aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact
    22981 cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg
    23041 gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc
    23101 tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta
    23161 tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac
    23221 gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag
    23281 tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc
    23341 ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt
    23401 taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa
    23461 aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt
    23521 agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt
    23581 caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat
    23641 ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga
    23701 agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac
    23761 atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa
    23821 taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa
    23881 atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta
    23941 tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat
    24001 acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa attattttag
    24061 acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat
    24121 tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg
    24181 tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata
    24241 ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga
    24301 tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca
    24361 gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga
    24421 gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg
    24481 atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta
    24541 cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata
    24601 cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact
    24661 tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata
    24721 tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt
    24781 tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca
    24841 tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct
    24901 tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta
    24961 cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct
    25021 tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca
    25081 ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca
    25141 acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt
    25201 gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat
    25261 tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa
    25321 tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct
    25381 gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata
    25441 aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt
    25501 cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg
    25561 ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc
    25621 atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa
    25681 cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta
    25741 tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact
    25801 gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta
    25861 gaagtttctt ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc
    25921 ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct
    25981 tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga
    26041 taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc
    26101 gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg
    26161 atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct
    26221 ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct
    26281 agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt
    26341 tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg
    26401 acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa
    26461 cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc
    26521 gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat
    26581 acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg
    26641 cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga
    26701 agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag
    26761 tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga
    26821 gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta
    26881 tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca
    26941 ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa
    27001 agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa
    27061 tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg
    27121 cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca
    27181 gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat
    27241 tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt
    27301 tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg
    27361 atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat
    27421 acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct
    27481 aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat
    27541 cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag
    27601 tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg ttttaaggta
    27661 cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg
    27721 ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc
    27781 ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat
    27841 agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt
    27901 tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca
    27961 aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt
    28021 tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct
    28081 ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg
    28141 attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct
    28201 tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt
    28261 tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg
    28321 attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca
    28381 ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc
    28441 gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca
    28501 tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat
    28561 tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg
    28621 attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc
    28681 cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg
    28741 atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta
    28801 aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg
    28861 cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat
    28921 tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca
    28981 ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg
    29041 atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct
    29101 tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca
    29161 tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc
    29221 atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc gactttctca
    29281 cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt
    29341 gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca
    29401 acgtattcaa cgacaccatc atgtttacat aacaaagcag ctcctgagtc atgcgctgat
    29461 ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga
    29521 cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata
    29581 catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca
    29641 atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt
    29701 ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct
    29761 gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt
    29821 gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg
    29881 atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg
    29941 tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggcgaaaaga
    30001 cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga
    30061 gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat caattgttga
    30121 ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt
    30181 gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt
    30241 cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc
    30301 attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt
    30361 ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca
    30421 gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca
    30481 ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca
    30541 acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga
    30601 cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa
    30661 cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt
    30721 tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt
    30781 gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt
    30841 tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc
    30901 ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc
    30961 caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact
    31021 tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc
    31081 ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct
    31141 tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca
    31201 gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca
    31261 taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca
    31321 aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc
    31381 aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt
    31441 ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga
    31501 ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt
    31561 ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc
    31621 gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc
    31681 cttttcttca tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc
    31741 aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat
    31801 gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga
    31861 aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca
    31921 actgaataac ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa
    31981 acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc
    32041 acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt
    32101 ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat
    32161 tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta
    32221 ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca
    32281 tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct
    32341 gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta
    32401 acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa
    32461 ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta
    32521 ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc
    32581 catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg
    32641 ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc
    32701 atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa
    32761 ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa
    32821 caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg
    32881 ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt
    32941 gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa
    33001 agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt
    33061 gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa
    33121 gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca
    33181 tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac
    33241 ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag
    33301 gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat
    33361 tgacagcttg tcttctaggc tttttaggga gtgcggcagc agggcttgtt tccggactgc
    33421 ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat
    33481 attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga
    33541 tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc
    33601 tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac
    33661 ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag
    33721 ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct
    33781 tgcttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg
    33841 gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc
    33901 tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac
    33961 tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt
    34021 ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca
    34081 aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa
    34141 cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat
    34201 catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc
    34261 tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga
    34321 acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt
    34381 aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg
    34441 tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg
    34501 gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat
    34561 tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc
    34621 agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga
    34681 gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag
    34741 tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca
    34801 gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa
    34861 agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc
    34921 caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt
    34981 cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga
    35041 tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc
    35101 cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa
    35161 agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc
    35221 ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt
    35281 gaaagtacaa acgatgatga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc
    35341 tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg
    35401 ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca
    35461 tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt
    35521 gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta
    35581 catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg
    35641 caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt
    35701 tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac
    35761 aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga
    35821 aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa
    35881 tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat
    35941 cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat
    36001 ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt
    36061 ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct
    36121 agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat
    36181 gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt
    36241 ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt
    36301 ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca
    36361 tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct
    36421 cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc
    36481 cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct
    36541 atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta
    36601 tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt
    36661 cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc
    36721 attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag
    36781 acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata
    36841 tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct
    36901 aggataccta cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc
    36961 atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc
    37021 aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt
    37081 ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa
    37141 atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat
    37201 ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat
    37261 ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat
    37321 aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa
    37381 aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa
    37441 aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa
    37501 tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga
    37561 tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat
    37621 attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc
    37681 actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat
    37741 ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg
    37801 atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc
    37861 aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat
    37921 gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat
    37981 ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt
    38041 actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat
    38101 cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc
    38161 gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DADQRX010000014        37186 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.014, whole genome shotgun sequence.
ACCESSION   DADQRX010000014 DADQRX010000000
VERSION     DADQRX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.014"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..550)
                     /locus_tag="J8V14_000711"
     CDS             complement(107..550)
                     /locus_tag="J8V14_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920837.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(566..1726)
                     /locus_tag="J8V14_000712"
     CDS             complement(566..1726)
                     /locus_tag="J8V14_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBA0920838.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2233..3933
                     /locus_tag="J8V14_000713"
     CDS             2233..3933
                     /locus_tag="J8V14_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920839.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4035..4868)
                     /locus_tag="J8V14_000714"
     CDS             complement(4035..4868)
                     /locus_tag="J8V14_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA0920840.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5172..7337)
                     /locus_tag="J8V14_000715"
     CDS             complement(5172..7337)
                     /locus_tag="J8V14_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0920841.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7597..9891
                     /locus_tag="J8V14_000716"
     CDS             7597..9891
                     /locus_tag="J8V14_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBA0920842.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9884..10549
                     /gene="pgmB"
                     /locus_tag="J8V14_000717"
     CDS             9884..10549
                     /gene="pgmB"
                     /locus_tag="J8V14_000717"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBA0920843.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10542..11549
                     /locus_tag="J8V14_000718"
     CDS             10542..11549
                     /locus_tag="J8V14_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBA0920844.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11571..12590
                     /locus_tag="J8V14_000719"
     CDS             11571..12590
                     /locus_tag="J8V14_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0920845.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12871..14238)
                     /gene="nhaC"
                     /locus_tag="J8V14_000720"
     CDS             complement(12871..14238)
                     /gene="nhaC"
                     /locus_tag="J8V14_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBA0920846.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14347..15768)
                     /locus_tag="J8V14_000721"
     CDS             complement(14347..15768)
                     /locus_tag="J8V14_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0920847.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(16011..17888)
                     /gene="tdc"
                     /locus_tag="J8V14_000722"
     CDS             complement(16011..17888)
                     /gene="tdc"
                     /locus_tag="J8V14_000722"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBA0920848.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18179..19435)
                     /locus_tag="J8V14_000723"
     CDS             complement(18179..19435)
                     /locus_tag="J8V14_000723"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBA0920849.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            20015..21316
                     /locus_tag="J8V14_000724"
     CDS             20015..21316
                     /locus_tag="J8V14_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBA0920850.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21385..21984)
                     /locus_tag="J8V14_000725"
     CDS             complement(21385..21984)
                     /locus_tag="J8V14_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0920851.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            22238..23119
                     /locus_tag="J8V14_000726"
     CDS             22238..23119
                     /locus_tag="J8V14_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920852.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            23132..24742
                     /locus_tag="J8V14_000727"
     CDS             23132..24742
                     /locus_tag="J8V14_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBA0920853.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(24819..25478)
                     /locus_tag="J8V14_000728"
     CDS             complement(24819..25478)
                     /locus_tag="J8V14_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBA0920854.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(25761..26426)
                     /locus_tag="J8V14_000729"
     CDS             complement(25761..26426)
                     /locus_tag="J8V14_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920855.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(26573..27178)
                     /gene="dhaL"
                     /locus_tag="J8V14_000730"
     CDS             complement(26573..27178)
                     /gene="dhaL"
                     /locus_tag="J8V14_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBA0920856.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(27206..28189)
                     /gene="dhaK"
                     /locus_tag="J8V14_000731"
     CDS             complement(27206..28189)
                     /gene="dhaK"
                     /locus_tag="J8V14_000731"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBA0920857.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(28191..28586)
                     /gene="dhaM"
                     /locus_tag="J8V14_000732"
     CDS             complement(28191..28586)
                     /gene="dhaM"
                     /locus_tag="J8V14_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBA0920858.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            28740..29060
                     /locus_tag="J8V14_000733"
     CDS             28740..29060
                     /locus_tag="J8V14_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBA0920859.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(29172..30041)
                     /locus_tag="J8V14_000734"
     CDS             complement(29172..30041)
                     /locus_tag="J8V14_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBA0920860.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(30137..31441)
                     /locus_tag="J8V14_000735"
     CDS             complement(30137..31441)
                     /locus_tag="J8V14_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA0920861.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(31438..31947)
                     /locus_tag="J8V14_000736"
     CDS             complement(31438..31947)
                     /locus_tag="J8V14_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920862.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(32122..34257)
                     /locus_tag="J8V14_000737"
     CDS             complement(32122..34257)
                     /locus_tag="J8V14_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0920863.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(34340..35995)
                     /locus_tag="J8V14_000738"
     CDS             complement(34340..35995)
                     /locus_tag="J8V14_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA0920864.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(35997..36311)
                     /locus_tag="J8V14_000739"
     CDS             complement(35997..36311)
                     /locus_tag="J8V14_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBA0920865.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            36481..36741
                     /locus_tag="J8V14_000740"
     CDS             36481..36741
                     /locus_tag="J8V14_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBA0920866.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            36755..37069
                     /locus_tag="J8V14_000741"
     CDS             36755..37069
                     /locus_tag="J8V14_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HBA0920867.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        10813 a         7731 c         6691 g        11951 t
ORIGIN      
        1 agattgtaac aactgcatat tcacacagtt aaattagtga cttcaaaatg aaataatgct
       61 cccagcaaaa aattaggctg ggagcattat ttcgttattc tgttcatcaa aaatcagcgt
      121 tttgattcca tttcatcaca agttcctcct catttactgc ttcatctttt tgctgtgttt
      181 gaattgtaaa accttgtgaa agatagaaat gaatagctga ttgatttttt tgatagactg
      241 tcaaagtcag tgttttattg gtttctttta tttttttcat tagttgcgta ccgattcttt
      301 gttgccggta ttcccttttc acaaagattc ctgcgataaa ctgatcaatc accccagcaa
      361 aaccaataat ttcttgatct atttctgcta agtaaatcac tgcatctggt aaagctttcc
      421 taacagattc tacttgattc tcccaataag ttgatggaat gaattcatga gaatccaagt
      481 tagcatgcag ccaaatcgat agaacagatt ccagttcttt ggcagacaat tttgttgttg
      541 tgcggatcat tttttctctc cttacttatt cattgatttt aaaataaaaa ccattttttt
      601 gaagctcttt tacaagtttt cgcccctcaa aaaagtatat cttctcttgt tttacttttc
      661 taataccaaa agaaacagca actttaggat catcagctga aacgattcga taagttccac
      721 caggcaattg atccagcttg actttcccat gatggtcgct ttgtactgaa aacggcacac
      781 catttctagt aagtgttttt ttcttatgtt tcaaaatgaa tgaactacca tttaccggtt
      841 tccctgacga atcactaata ataaatgaaa aatcaagcac ttttttactt aatctatact
      901 gatagattag tcgtccgata aacactaaca gtatgactac agctattatc gttcctgcaa
      961 gaataaacca ttgccgcatg gttctttcct gatttccttt tccttcttct ttttttaccg
     1021 attcgttata aggaatccga tgtcctgtca ctagcaaacg atgcgtgttg atcatatacg
     1081 gcgtacatgt aagcaaggta actaagtctt ctccttctga aatcgttaga acagacgtgt
     1141 catctggcag taccacttgt atatttttaa cttgataagc caatttctcc cccaaaacag
     1201 tcaaaataaa cgtgtctccg ttttctaaac gatctaattc ggtaaataac cgtcgattag
     1261 gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt tccaccaagc gggaaagagg
     1321 ttccttgcaa gacgcctgct ccattttcaa ggatcctacc attgagccga tcataaagtg
     1381 aaatatttaa ttggatctta ggtatattga tacttccgat cagccattcc tctaattggc
     1441 tggatgttgt actttcattt ctgttctttt cattgaaagg atcagtacca ggattgaatc
     1501 cttggtcagt taatcgttgg tttttctcct ccattttctt tcgctgcact tcaatttcag
     1561 ctgcggtccg tttttgtact tgttcgatac gtttttgatc aataaaacta tttaaagaat
     1621 ccacataaaa tggatataaa gcgatcagta accctacgac gatacatatt aataaaaaca
     1681 aacgaaacaa aagtcgacga gactgttttt gcttactctg cttcacatgt gtcctccctt
     1741 gcaagttttt ttgttctttc ttttattata tgggttcagg aaaaaaatag aaaatgaaag
     1801 ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata agtacagccc aaaaaaatag
     1861 ctactcatat tttttgttgg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     1921 ttaaggaaga ggttgtgaaa actgcgttat gctttgagta ataagagcgc tagtcagaaa
     1981 atagcttctc atattttcta ggctagtgag cttattaccg agaagcagct gattgaacac
     2041 cgtttaccga gaggtcatga aaaagctact ttgtttcgag tattaagaaa aattctgttt
     2101 caaaacaagg agatgcaaca aaacttatgt cgcatcctct ctccttagaa ctttcactta
     2161 tgtttttctt gtaaaattca taaatatttt catgaatttt ctaatttctt gcctacctct
     2221 cgcataggat atatgatgaa tttatctaaa gtttgcaaag gagttcgaca attgaaaaaa
     2281 tatggcccat ttttagcaat ttttttcatt gctttggcat ttctcatgcc tcatttattc
     2341 acacataaac taattttggg tgctgatgcc ccattccatt acaatcggtt ctatgatgcc
     2401 gcgcaacaaa ttaaatatgg ggatttccat tattttcctt ccctttatgg attccagcaa
     2461 agtggcagga tcgtgaatgc cgtttacggc ccatttttcg cttatttaca aggattgatc
     2521 gtgttgcttg caggtagctg gtttcattat cagattcttt caaatttcat tctttatctt
     2581 atttcaggat tttctatgct gattttactg agatatgtca atgttcgaaa ttgggtaagt
     2641 gtaccagtag ccgttttatt catgagtacg tattctattc aatactggtc tatcaaccaa
     2701 ggattcacta gctgggggac ttgtttttat cctttatgta tgatccctat tgctgatttc
     2761 gtccttcata aaaagttccc tgttatcaaa gtcgctgtct cagtgggact tatgacacag
     2821 atccatttac tgtcttcgat catgttagct ttgatgtata tcccttttta tctttattac
     2881 ttttttaacc agactgaaaa aggaaaagca ctgcttcaac tgattaagtc ggtcttgctt
     2941 tattttgttc tgacagcaaa tatttgggca ggattagcta ttgtttacag tgggaataag
     3001 attcttcctc cctttgttaa cagagaaatg agtcaaaagg cgatagatct ggatggcagt
     3061 tattggctcc attaccctaa ggcctttcct atattgatat tatcaggttt tttgcttttt
     3121 attttctttc aacaaaagaa caccttattc caaaaaatcg tgttgtacac aagtcttgtt
     3181 ttcttttttc tttctactag cttgattcca tggacgacat tgatcgaaaa aaatgtgcct
     3241 tttattagtt tgatccagtt tccatttcga tttttcgtgc catttacgct attgcttcta
     3301 ctttaccttg ccctttcact gaaccagtgg tctgaacaaa agtggttccg tcttttaagt
     3361 atatttggca ttcttatttt ttcagcacag acgatccaga acttatacca gcatctggaa
     3421 aagtgggaaa atgaaacatt tgtcagtcga catacttatc tattcgatac gccggaagaa
     3481 gcaaggaaaa cattcttcag caaagatatg tctaccagct tgttcgtttt ccaaaaaacc
     3541 acacctgact atctgccaat ctataaggag acaaaagaca ataaatacga ccggtataac
     3601 gaagaaatac tagaaaacga aacaactttt ataaaaacac atccaggcgg tcttttgaca
     3661 atcaagtgga aaaacactgg ggacaaaact gttcatatcc ctgttatcgt ctacgatcgg
     3721 actgtgctac agcaaaatgg aaaaacattg actgattatg aagtaacaga tatcgggacc
     3781 ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc ttcattatca aacgccaatc
     3841 tatttctatt tcattctttc tcttactttg attggctggt ttaccttgct atgtttattt
     3901 atttatcata gatacaagct tttacgtgct taggcaaaag ggataaatac atccgatcaa
     3961 aaaactctcc aaacggtata atcgtttgga gagttttatt ttaatcagtt tttttatgtt
     4021 atatgttata ttttttattc gttcacatct aattcgattt ccaccccgta gtgatcactg
     4081 atgattggac tatcgatccc atcaaaaatc cgttgatatt ttttaacctg cattccttca
     4141 gggacgaaaa taaaatctat acgcaatttt tctgtatttt gttcccaacc atctattttc
     4201 ttttccacag tagctgcttt tcctttttca ttcgcgacaa catacgaatc ttgaagaaga
     4261 aggcaacttt ctcttacagt ataataacct tgagagtcta ctggattatt gaaatcacct
     4321 aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt ccaagttttt ccactcaaaa
     4381 taaaagccac tttttttatc tgtccaccaa gaaaagtggc agctcgagac aaaaatgtcc
     4441 tgatcatcta tctttgtttt cccaatcaag atcttgcgtg tctgataatc ttctttcttt
     4501 tgaaattggg agacggtaag gacctgactt tccaatgttt ttttcgtaag cagtgcattt
     4561 ccttcttcat atttatcata accgatgtgg ctcatttccc aactccaaaa atactctttt
     4621 ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat tatctttacg gataggtact
     4681 tgattcttta ccggacagaa cttcattagc ttcgcaggtt ctacttcttc agattccagt
     4741 aattggttga tttcttgtaa agcaatcact tcataatctt ctaacaggat cttatctgct
     4801 aattcttgca attttttctc tggttctggc tcaagccagc tatgcgtgtt gagtgtcatg
     4861 aatttcattg gttttctcct tgtcttcatt tcgaaacaaa agggttgtga caaaattttt
     4921 gtcacaacct ctccagcaaa tagtgttctc ctgctaactc ctcagtaata agctcaatgg
     4981 accaggagct attttttgtt tggctgtttt tatcacttgg tgcagggcag ctttttcacg
     5041 acctctccac taaacagtat tcaaactgct gcttctcggt aataagccca accaaacgaa
     5101 aaataggagg agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     5161 acaacctctc cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat
     5221 tgcttgatca ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct
     5281 ttttcttcgt tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact
     5341 tcttggcctt ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt
     5401 cccatatgga tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta
     5461 gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata
     5521 acagcaaagc cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa
     5581 ggaatcactt ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta
     5641 ctacctgctt gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat
     5701 tgcagcagat cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg
     5761 actccattgt ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc
     5821 cctacttttt ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct
     5881 ttgatatttt cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt
     5941 cctgttccgc tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt
     6001 cccggtgtag caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca
     6061 ccaaaagcga tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata
     6121 gctaacggtg tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag
     6181 atatctgcca tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca
     6241 gcagcaaaca taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac
     6301 gctgctgaaa aatacattga tttatatctt gctttcttat caggatcgac attacgatac
     6361 atcgcaaaag ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa
     6421 cgagccggcg tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta
     6481 agattcacta aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc
     6541 cccgcctgtg cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga
     6601 atcgtcagca tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa
     6661 aacggtgcta gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg
     6721 attccagcct gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat
     6781 aagataacaa caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat
     6841 tttctataat tatagtactt attataagcc attgccccga caaatccagc gatgatccct
     6901 acgaatactc ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc
     6961 atgatcttcg taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt
     7021 gtgacaccaa agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca
     7081 aatgctccgc ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata
     7141 tgaagattac caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc
     7201 ggcgtccaat caggattgat caagggaatt gatttcccta tactgatcat caacccagct
     7261 gccggcataa ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa
     7321 ctaaatatct ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta
     7381 tagcgtgcgt tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct
     7441 ataaaaccta taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta
     7501 agcttttttc tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat
     7561 tatgaacgta taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt
     7621 gctattgatc catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa
     7681 gaatctttaa caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact
     7741 tactcagggg accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca
     7801 cgtgtcggct ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg
     7861 aactttatcg cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa
     7921 ccagaagact tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca
     7981 atcactactt ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa
     8041 aaagaatcag cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa
     8101 ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt
     8161 tggaatgaga ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt
     8221 ccaaacaact ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat
     8281 ggaaaatcag tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct
     8341 gcagaattat ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt
     8401 agagatattc cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca
     8461 aacaattata tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt
     8521 gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc
     8581 caactattct ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc
     8641 acaggagaaa aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt
     8701 tatcttgctt tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa
     8761 ctgccgcaag caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg
     8821 gtcaccttca ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac
     8881 cgaaatggcg cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa
     8941 tatttgaaaa atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt
     9001 gtccattttt caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa
     9061 tatgagaaca atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt
     9121 tacacgcgag aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa
     9181 gaactagcca aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta
     9241 ggaatctttg tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt
     9301 cccacaagcg atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc
     9361 ttcatcaagc aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa
     9421 gaagaaaagc aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt
     9481 tcgccaagta tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa
     9541 atgtaccagc gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc
     9601 ttgcacatca cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg
     9661 aaaactgccc atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat
     9721 tctttccata tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt
     9781 actttcgagt tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct
     9841 ttagcggatc gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt
     9901 gtttgattta gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa
     9961 gcttggtcag gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt
    10021 cagccgagaa gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc
    10081 aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca
    10141 gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga
    10201 agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa
    10261 aatgggtctt acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa
    10321 acctgcgcca gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg
    10381 tatcgggatc gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc
    10441 aattggcgtg ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac
    10501 ttttcttacg atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct
    10561 ccgtcgtacc agttccagag actgaaataa agaaaatcac attgaagaac gactacttag
    10621 aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag
    10681 gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa
    10741 gcttttttgg agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg
    10801 agcatagatt ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt
    10861 ctttccaatt ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt
    10921 acttaaaaga tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt
    10981 taacaaaaaa cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca
    11041 atccgaccaa ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac
    11101 atcagttaac ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg
    11161 gaaattggac aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag
    11221 agatccttgc atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc
    11281 tttccaaaac ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa
    11341 cttctaataa aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca
    11401 atggaaaaca gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata
    11461 tcgttcattt ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc
    11521 agacgattta tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta
    11581 ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca
    11641 acgatcatcc tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac
    11701 taggttatgt tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg
    11761 gcgtaatctt accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga
    11821 tcatggaagc aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg
    11881 ctaaaaattt tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag
    11941 atggttttat tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa
    12001 accacatccc tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg
    12061 tagataatga taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac
    12121 ataagaatat cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt
    12181 tcggttatca aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag
    12241 agcatccgga ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat
    12301 tggtggtcat tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct
    12361 atgtcattcc agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa
    12421 cacaccctta ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc
    12481 aaaagctgcg cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc
    12541 atcgcttgat caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact
    12601 cactaaatac gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact
    12661 tctaacacat tgtttatctg taaaaaagag cgtgggacaa aagtaaagaa cacttttgtc
    12721 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctga
    12781 aattcacaaa aatttaaaaa gcaattttct tgagtttctt cttatttctt ggagttaaac
    12841 gcttctgtcc cagcctcttt tttccttcct tcaatttttt acacgtcgac gattcaataa
    12901 aaaaccgcct aatattgtaa agacaggtgc aagaatcgag aaaaaggcga atggcaagta
    12961 atgcaacgcg ctaactttca tcgttcccat aatgaatgtg ccgctgactc cccacggtac
    13021 gagagaattg acggtcgcac ctgaatccgc tagtgttcgt gtcaaatagg tttttgataa
    13081 gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga agaatgatgg acagatactg
    13141 ttctccaacg atcagattga ttccgataca gctcaaggca gtaaacccga ctaaacgaga
    13201 aggtcggtcc atcttttctt ttaattcttg aacgatggta tcaacgatca gatatttgat
    13261 cagtaaccca cctaatgcca atgcaagtaa aatcaaacta gcagaaccta acatactcat
    13321 gatcccgcct cgagaaagca gttcatctag ctttggatca ggtgtatttg cgacataacc
    13381 attcatcaag atgtctgctg cttttccgac cgtcaaactt ttctcatgga taaaagaaag
    13441 aacgagagca gctgtcgatc ccgctagtaa tgtagggata gccggaattt ttttgattgc
    13501 ccccccaaat aaaataacta gtggaatcaa tgtccaagga gagatccaaa agctcgcttt
    13561 catgttttgg atcatttcat tgatactagc tagatcggct gaatgactgt tatgtcctaa
    13621 tatcgtataa aaaatcaacg aaaggacgaa tgctgggaaa tccgtataac gcataccagc
    13681 gatatgttcg aatagattaa ctcctccaat acccgctgcc aagttggtcg tatctgataa
    13741 aggcgagcag ttattcccta aaaaggcacc ggaaacgact gcgcctgtag tcattgcatc
    13801 attgaatcca agtaaatgac cgatccccaa aaaagcaatt ccaatcgtcg aaatcgttgt
    13861 aaatgaactg ccgacagtca ctccgacaat tgcacaaaca agaaatgctg tcggtaaaaa
    13921 gaaacgcaca gaaacaagtt taaatccata aaccataatc gtagggatgg ttccagataa
    13981 aatccatgaa gcaactaaga caccaatcaa aagaaaaatc aggattggaa tgatccctgg
    14041 agtgattcct ttgatgatcc cgtcatggat ctcatcccat gtgaactttt taaatctgcc
    14101 ataaaacaac aaacacatga aagctgcaag gatcgggata tgtggcgaga gctggaatcc
    14161 gatgatcagt actcctagga gtgccaacaa cacgatcaat acagcgatac tttcttttaa
    14221 agaaatattt ttattcatcg tcgatctcct atttcaaatg aaaaggccct gccatgaaaa
    14281 aactattcac ttggcaggac ctcctactaa atcagcgaca aaacgtcggc tgaaagactt
    14341 atttttttat ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa
    14401 gtgattgatg atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc
    14461 atcactcaat gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag
    14521 catgcagctg actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc
    14581 gaattctgga tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat
    14641 acaataaata acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat
    14701 tacaccataa agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct
    14761 agatacgccg tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg
    14821 agctacttgg atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac
    14881 accaataaat accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg
    14941 acagtcaatc aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac
    15001 atttgctaca agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa
    15061 tacgcctttt gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat
    15121 ttcgatccca acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc
    15181 caagtcaggg aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc
    15241 gactttgaag gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc
    15301 tagccataca ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt
    15361 gatgatccag tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc
    15421 tacattacca aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc
    15481 tgggaacatt tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc
    15541 tgtttttacc cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga
    15601 aaccattgat atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt
    15661 ccaaccgact gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag
    15721 accgataaag gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct
    15781 ccttgatttt ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca
    15841 attcagcgaa tatttgaggg gattctttga gtttattgct tgaagggttt cttcaaagac
    15901 aacctcaaaa caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat
    15961 gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct
    16021 tcgcttgcca ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg
    16081 attttttcag catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg
    16141 gcacgcaata cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg
    16201 ttcacaaatt gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt
    16261 aagaactcat taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg
    16321 ttcaatttat tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc
    16381 atattgaaat ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat
    16441 gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat
    16501 ttaccatatc ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct
    16561 gttgcccctg cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg
    16621 cctttttcaa atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg
    16681 atgacgatac caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca
    16741 gattctgctt cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg
    16801 ttttctgtaa agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag
    16861 ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt
    16921 aagtagaagt aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata
    16981 ccgtcgattg ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt
    17041 ttttcagctg ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg
    17101 tggtcaactg gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt
    17161 agccatgaat agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt
    17221 ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga
    17281 ttcatgattt cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt
    17341 tcaggtgtca cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt
    17401 ccttctaagt ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc
    17461 attaattttg caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat
    17521 tcataggcaa cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc
    17581 atcaatgttt cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa
    17641 tgggtacgca tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt
    17701 tcaaaactag cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag
    17761 ttttgacgcc agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg
    17821 ccgttctcag ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat
    17881 tcactcatag tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac
    17941 ctgttcttct attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta
    18001 tcaattatcc gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa
    18061 tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt
    18121 tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct
    18181 aatcaattac tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac
    18241 catcgaattc atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa
    18301 tacttatagc tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg
    18361 tggaaacaag cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt
    18421 taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg
    18481 cttgcgttat tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt
    18541 cttctgccaa acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta
    18601 agtcttcgat ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc
    18661 gatcatcttg attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa
    18721 gccaaacggc accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg
    18781 ggatcgttaa accgaacgct tctgcttctg gtccttctaa ttttcggatt aggtctaatc
    18841 cagctgtgat atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt
    18901 tatgcagatg caaaaagtcg atagattgca ggatttggta agtaaactct gtaaatgaga
    18961 ttcctacttc caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt
    19021 ttttaccgta gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt
    19081 tcacaaatgt gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt
    19141 ccacgttatg ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg
    19201 ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt
    19261 ggaatctttt catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg
    19321 gatcaacgcc gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt
    19381 cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac
    19441 tgacctccgt gtagtttttt tatgaaagca ggaacctgct agcatatgta attaattatt
    19501 tcttcaggat aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat
    19561 ctcgcggtac cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg
    19621 ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac
    19681 tggtttgcag cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat
    19741 cttcgtcttg ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca
    19801 gaaaaaggat atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg
    19861 aacattttat tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt
    19921 tatcgagaag tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac
    19981 aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt
    20041 aggagccact tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg
    20101 atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga
    20161 accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga
    20221 ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca
    20281 acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac
    20341 tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc
    20401 tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat
    20461 cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga tccaaaaaga
    20521 cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt
    20581 tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg
    20641 attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga
    20701 aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt
    20761 ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta
    20821 tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc
    20881 taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg
    20941 tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca
    21001 gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga
    21061 catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca
    21121 aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac
    21181 ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga
    21241 cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc
    21301 acgcgaatct gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat
    21361 cgtcagtttc ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac
    21421 ttctgtccat tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc
    21481 ttctaccatt gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt
    21541 tccttctttc cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata
    21601 cgcatccatc gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc
    21661 gatataaaat tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat
    21721 cagtgtttct tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc
    21781 attcataaat gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa
    21841 aatcgtcgct ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt
    21901 tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg
    21961 tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt aattttaaac ttttataata
    22021 tatatagttc aaatttattc tgaggtttat tataactatc tattgagaaa gtatcaagct
    22081 agcaattcct tgtttatgaa ttcattgaca gaaatttata aaaagaatat tatgtactaa
    22141 aataataaaa aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg
    22201 tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac
    22261 acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca
    22321 ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg
    22381 gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt
    22441 atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt
    22501 gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg
    22561 gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc
    22621 aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga
    22681 ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat
    22741 tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca
    22801 tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact
    22861 cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga
    22921 aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa
    22981 aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc
    23041 gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat
    23101 gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac
    23161 ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt
    23221 cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt
    23281 agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc
    23341 agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt
    23401 ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa
    23461 agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc
    23521 tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg
    23581 tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct
    23641 atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat
    23701 gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct
    23761 acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc
    23821 ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt
    23881 cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg
    23941 ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg
    24001 tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat
    24061 gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt
    24121 aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat
    24181 catggtagtc ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt
    24241 tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca
    24301 actttattgg accaatatgt tactagctat tctatttgct atattggcaa tcctgattag
    24361 ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct
    24421 attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat
    24481 tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc
    24541 ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa
    24601 aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt
    24661 ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg
    24721 aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc
    24781 tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct
    24841 ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat
    24901 ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt
    24961 ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca
    25021 gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt
    25081 aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt
    25141 tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat
    25201 aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt
    25261 actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat actgccggag
    25321 gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca
    25381 tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac
    25441 aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag
    25501 ctatttctaa taaagaaaca taacttccct tattccaact gataattttc ttttatcata
    25561 gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc
    25621 tttattttcc ttcttttgag accttaagta attgattcct atcaattttt taaatattta
    25681 ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg
    25741 cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt
    25801 gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag
    25861 aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt
    25921 ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt
    25981 tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca
    26041 agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac
    26101 atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct
    26161 tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc
    26221 cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg
    26281 agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc
    26341 ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac
    26401 tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta
    26461 cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat
    26521 aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt
    26581 gattgatcac atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga
    26641 tatgtccgat tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt
    26701 tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct
    26761 ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc
    26821 tttgaatacc cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag
    26881 aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag
    26941 aagccccacc gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg
    27001 tttcaggatg atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat
    27061 ttccatggtc tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat
    27121 tgattgcctc tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat
    27181 tatcaccctt ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag
    27241 aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta
    27301 gtgaggtcat aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca
    27361 gagctaaaac atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt
    27421 tgactagtac acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa
    27481 gttcctttgc cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt
    27541 ggataccaac accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt
    27601 ctggtacagt tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt
    27661 ctcccagttc tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt
    27721 tgtgtacaag gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa
    27781 cggcgatatc atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt
    27841 cctttgccat atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc
    27901 cttttccact atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa
    27961 acacatcacc aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg
    28021 gttcatgtcc gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg
    28081 ctacaacacg ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt
    28141 ccagcatttc ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat
    28201 cctttctttc cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg
    28261 gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt
    28321 tgctgctgct cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg
    28381 atatccgcaa aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt
    28441 ttcatcgggt ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct
    28501 tcggattttt gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga
    28561 gaaataagca aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg
    28621 attggtttcg ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa
    28681 aatatgtgta gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa
    28741 tgattacaac aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg
    28801 tttttggtga ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa
    28861 atatctttgg cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag
    28921 cacttgccga aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa
    28981 aaatggacta tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa
    29041 cagctgtctc cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac
    29101 atgtagtggc actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca
    29161 tcctctcttt tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc
    29221 gcccctgcta aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga
    29281 cttgttgatt tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc
    29341 actgattttt ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag
    29401 tcagcttttt ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg
    29461 attgcttgac tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt
    29521 tgagcaacct gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga
    29581 tctgtttgga cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca
    29641 cggtcatttc ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct
    29701 tctttcaatt tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc
    29761 aagtcttgca aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct
    29821 gaagatgaaa tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc
    29881 gaggcaccgt tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt
    29941 tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc
    30001 cagattaacg gcttcattct ttttttcatc acttttccca tccttactac tttttaacta
    30061 atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta
    30121 aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat
    30181 aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta
    30241 tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat
    30301 catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga
    30361 acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat
    30421 gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac
    30481 tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca
    30541 cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc
    30601 gatatagcat cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa
    30661 cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat
    30721 cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac
    30781 tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac
    30841 ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat
    30901 ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa
    30961 tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg
    31021 caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca
    31081 cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg
    31141 aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact
    31201 cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg
    31261 gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat
    31321 aacaccagac accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa
    31381 aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca
    31441 tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt
    31501 ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc
    31561 cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa
    31621 agatttattt acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt
    31681 aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt
    31741 ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa
    31801 aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg
    31861 ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc
    31921 aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag
    31981 gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa
    32041 aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc
    32101 tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc
    32161 tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa
    32221 tggatataag attacatgat caaattccag tccttttgct agactcaaag gaatgatttg
    32281 gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc
    32341 ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag
    32401 tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg
    32461 tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt
    32521 gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta
    32581 gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt
    32641 ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg
    32701 tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac
    32761 cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt
    32821 gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg
    32881 ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa
    32941 aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa
    33001 taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt
    33061 tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag
    33121 cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt
    33181 agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa
    33241 caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg
    33301 ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc
    33361 tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa
    33421 tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa
    33481 gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat
    33541 agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc
    33601 ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt
    33661 ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa
    33721 aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc
    33781 gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc
    33841 ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc
    33901 tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc
    33961 aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg
    34021 tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag
    34081 gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg
    34141 gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag
    34201 cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca
    34261 cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat
    34321 gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt
    34381 gatataaaga aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc
    34441 tttccgctag atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt
    34501 ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt
    34561 tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt
    34621 ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg
    34681 catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt
    34741 ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga
    34801 tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg
    34861 gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt
    34921 ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta
    34981 aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt
    35041 gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg
    35101 gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat
    35161 cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc
    35221 cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa
    35281 tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc
    35341 cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca
    35401 attctttttc tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt
    35461 gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga
    35521 tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt
    35581 tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa
    35641 tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt
    35701 gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt
    35761 catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa
    35821 gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa
    35881 aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa
    35941 gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat
    36001 ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca
    36061 taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc
    36121 acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt
    36181 ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct
    36241 ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat
    36301 agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa
    36361 acaggagctt tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat
    36421 atttgcataa aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt
    36481 atggagattg ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt
    36541 ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc
    36601 gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat
    36661 tgctttgaga aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc
    36721 aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga
    36781 attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga
    36841 aacgacgatt gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa
    36901 tgcagaattc ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc
    36961 taatcatatt tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct
    37021 tgatttagtt gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt
    37081 tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg
    37141 gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca tcaagt
//
LOCUS       DADQRX010000015        37103 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.015, whole genome shotgun sequence.
ACCESSION   DADQRX010000015 DADQRX010000000
VERSION     DADQRX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.015"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            347..2008
                     /locus_tag="J8V14_000742"
     CDS             347..2008
                     /locus_tag="J8V14_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0920868.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2078..2425)
                     /locus_tag="J8V14_000743"
     CDS             complement(2078..2425)
                     /locus_tag="J8V14_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBA0920869.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2509..3450
                     /locus_tag="J8V14_000744"
     CDS             2509..3450
                     /locus_tag="J8V14_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920870.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3451..4494
                     /locus_tag="J8V14_000745"
     CDS             3451..4494
                     /locus_tag="J8V14_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0920871.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4500..5570
                     /locus_tag="J8V14_000746"
     CDS             4500..5570
                     /locus_tag="J8V14_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920872.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5560..6513
                     /locus_tag="J8V14_000747"
     CDS             5560..6513
                     /locus_tag="J8V14_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0920873.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6525..7535
                     /locus_tag="J8V14_000748"
     CDS             6525..7535
                     /locus_tag="J8V14_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0920874.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7820..8641)
                     /locus_tag="J8V14_000749"
     CDS             complement(7820..8641)
                     /locus_tag="J8V14_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBA0920875.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8738..9016)
                     /locus_tag="J8V14_000750"
     CDS             complement(8738..9016)
                     /locus_tag="J8V14_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920876.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9284..11293
                     /gene="metG"
                     /locus_tag="J8V14_000751"
     CDS             9284..11293
                     /gene="metG"
                     /locus_tag="J8V14_000751"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBA0920877.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11772..12359)
                     /locus_tag="J8V14_000752"
     CDS             complement(11772..12359)
                     /locus_tag="J8V14_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920878.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12534..13856
                     /locus_tag="J8V14_000753"
     CDS             12534..13856
                     /locus_tag="J8V14_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0920879.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14011..14787
                     /locus_tag="J8V14_000754"
     CDS             14011..14787
                     /locus_tag="J8V14_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBA0920880.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14780..15349
                     /gene="rnmV"
                     /locus_tag="J8V14_000755"
     CDS             14780..15349
                     /gene="rnmV"
                     /locus_tag="J8V14_000755"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBA0920881.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15342..16226
                     /gene="rsmA"
                     /locus_tag="J8V14_000756"
     CDS             15342..16226
                     /gene="rsmA"
                     /locus_tag="J8V14_000756"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBA0920882.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16677..17306
                     /locus_tag="J8V14_000757"
     CDS             16677..17306
                     /locus_tag="J8V14_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920883.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17430..18539)
                     /gene="ugpC"
                     /locus_tag="J8V14_000758"
     CDS             complement(17430..18539)
                     /gene="ugpC"
                     /locus_tag="J8V14_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBA0920884.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18748..19170
                     /gene="mgsA"
                     /locus_tag="J8V14_000759"
     CDS             18748..19170
                     /gene="mgsA"
                     /locus_tag="J8V14_000759"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBA0920885.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19493..20029
                     /locus_tag="J8V14_000760"
     CDS             19493..20029
                     /locus_tag="J8V14_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBA0920886.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20069..21832)
                     /locus_tag="J8V14_000761"
     CDS             complement(20069..21832)
                     /locus_tag="J8V14_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920887.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21835..23550)
                     /locus_tag="J8V14_000762"
     CDS             complement(21835..23550)
                     /locus_tag="J8V14_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0920888.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24329..24553)
                     /locus_tag="J8V14_000763"
     CDS             complement(24329..24553)
                     /locus_tag="J8V14_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920889.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24753..27521
                     /locus_tag="J8V14_000764"
     CDS             24753..27521
                     /locus_tag="J8V14_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0920890.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27602..28021
                     /locus_tag="J8V14_000765"
     CDS             27602..28021
                     /locus_tag="J8V14_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBA0920891.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28033..28503
                     /locus_tag="J8V14_000766"
     CDS             28033..28503
                     /locus_tag="J8V14_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0920892.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28520..29299
                     /locus_tag="J8V14_000767"
     CDS             28520..29299
                     /locus_tag="J8V14_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0920893.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29286..30107
                     /locus_tag="J8V14_000768"
     CDS             29286..30107
                     /locus_tag="J8V14_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA0920894.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30124..31173
                     /locus_tag="J8V14_000769"
     CDS             30124..31173
                     /locus_tag="J8V14_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA0920895.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31186..32190
                     /locus_tag="J8V14_000770"
     CDS             31186..32190
                     /locus_tag="J8V14_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA0920896.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32823..33842
                     /locus_tag="J8V14_000771"
     CDS             32823..33842
                     /locus_tag="J8V14_000771"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBA0920897.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33885..34766
                     /gene="galU"
                     /locus_tag="J8V14_000772"
     CDS             33885..34766
                     /gene="galU"
                     /locus_tag="J8V14_000772"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBA0920898.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35066..35443
                     /locus_tag="J8V14_000773"
     CDS             35066..35443
                     /locus_tag="J8V14_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920899.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36049..36663
                     /locus_tag="J8V14_000774"
     CDS             36049..36663
                     /locus_tag="J8V14_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA0920900.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        12149 a         6500 c         7581 g        10873 t
ORIGIN      
        1 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttattc agataaggga
       61 ctgtgacaag acttttgtca cagtttcttt ttgtttattc ttttttatct ttgtatggca
      121 ataatagaca atataacgaa aattatttaa tggcttttgg aagaccatgg aaaaccttgt
      181 acaaagctat tttttctgaa aaattccttg aaacctcacg aaaaaatcat tattttatga
      241 aaaagacaaa aaaaagtgtt gattttttaa taattattca ttaaactctt aaacaacttc
      301 agaaaatatt aattattctg aaaattccaa taaaggggta cgtaaaatga aaaagaaatt
      361 tacatttggt ttggttgcgg tatgcggcct tgtacttgct ggatgttatg gcggcagttc
      421 agccacaagc agtagttcag caaaaagcgg ttcagcagat ggaggaggcg tatttaactt
      481 agtcgtgcct caagaaatgc ctacagctga cttatcagtt gcaacagata caatcagttt
      541 tacagcattg aataacgttt atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca
      601 gccggcagga gctagcgagt tggcagaagt cagtgaagac ggattaactt acaaaatcaa
      661 attacgagaa gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg attacgttta
      721 tggctggcaa cgaacagtag acccagctac agcttctgaa tatgcttacc tgtatgcgcc
      781 agttgaaaat gcagaagaca ttacagctgg aaagaaagat aaatcagaac tagggatcaa
      841 agcagttggt gactatgaat tagaaatcaa attgacgaaa caaacaccat atttccaata
      901 tctgcttgct ttcccatcat ttttcccaca aaaacaatcc gtagtggaag aaaatgggga
      961 tgcctatgca tctgcaagtg ataaagctgt ttataatgga ccattcacat tagaagattt
     1021 tgatggacca ggaacagata cagaatggac ttataagaaa aatgatcatt actgggacaa
     1081 agatacagta aaactttctg aaatcaaagt cagtgtagta aaagaatctt ctactgcatt
     1141 gaacttattc aaagatggcc aagcagatga cgtgatcttg tctggtgagt tagcacagca
     1201 aaacgcaaac gatccagctt atacttctgt taaagaagcg cggacaagct atatcgaatt
     1261 caatcaacgt gaaaagaatt caccattcaa caatgtgaac ctaagaaaag ctatttctta
     1321 ttctatcgat cgtgaagctt tagtaaaaca agtactaggt gacggatcag ttgtctctac
     1381 aggattgatc ccatcagata tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga
     1441 agctggaaaa ttagtttctt atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa
     1501 aaaagaattg ggcgttgatt ctttagaatt tgatttgatg gcttctgatg atgattcatc
     1561 gaaaaaagta atcgaatatg ttcaaaactc aatccaagaa aacttggatg gcgtaacagt
     1621 caaaccaaca cctgttccat tctctgtacg tcttgaccgc tcaacttcgg gtgattttga
     1681 cgcagtacta ggcggatggg ctgctgacta tgcagaccca agcagcttta ctgatttgtt
     1741 tgtcacaggt aattcataca accgtggtcg ctggagcaat gcagaatatg acgaagcagt
     1801 taaagcagct tcatcaacag acgctggcga tgaacaagcc cgttgggatg atttccaaaa
     1861 agcaatcaag atcatctctg atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg
     1921 tcacctagtg aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg cttcatggga
     1981 ttacaaatgg acatacgttg aagaataatc gttaataccg tgagcatgag aaaaaacgag
     2041 gctgcggcaa atgtcgcaac ctcgttctca tattcactta tcctaacaaa gaaaaaagca
     2101 tgaaaatagc agaaagagca attagtattc cggcaatgat cagccaaaag ctgtacatac
     2161 cgtagctggc agacgagagc gaggaaaatt ctggtttttt ctttcggttc cttgttcgtg
     2221 caagatggac cgatcgctga aaatgatcga agaaacccga agaaaagacc cataaaaaac
     2281 cgccgataat cagaaacggc aatgaccata agaatagatc attagataat tgaatgagtg
     2341 taaaggaatg gttgatgaga ttttttatta aaataaccag aatcagtaga aaagctactg
     2401 cgtaggggat agattttttt ttcatttgat tgctccttta tttttggttc attatctaat
     2461 aatgtagcga aacatctgtc agaagtcaaa agctaggggg aaacattagt gaatagttat
     2521 atcaaatatg tattaaaacg tgtctttttc atgatcatca cgctttggtt aattgcaaca
     2581 atcacctttt tcttgatgca attgctgccg ggcacgccat atacgaatca ggaacgtttg
     2641 agtcctgaaa caatcgagat gctaaataaa caagtcggtt tggataaacc agttatcgta
     2701 caatatggga tttatctttc taatttgctt caaggagatt ttggaatttc cttccaattt
     2761 aaaaaccagc cagtcgcaca tttgttagct ggaagaatcg gaccatcctt gcagttaggg
     2821 ttacaggcaa ttatctttgg aacagtccta gggacgattt taggaacgat ctctgccatg
     2881 aagcaaaata catgggcaga tacatcttct acactcgtag cgatattagg tcgttccatt
     2941 ccaaactttg tatttgctgt tttattacaa tatatctttg caatcaaatt acaagttttg
     3001 ccaatcgcaa aatgggatgg ctttgtgtac acaatcttac caacaattgc gttggccatg
     3061 tcgccgcttg ctgactctgc tcggtttatc cgtacggaaa tggtggaagt actgcatagt
     3121 gattatgtgg aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat
     3181 ggtcttcgaa atagtttgat tccattgatg actttattag gaccgttagc tgtagcattg
     3241 atgaccggat cactggtggt agaaaatatc tttgctattc ctggtatcgg ggaacaattc
     3301 gttaagtcaa tcacaacaaa tgattatcct acgatcatgg ctgtaacgat cctttactca
     3361 tttatgctga tttttgttat tttgatcgtg gatctattgt acggtctagt tgatccaaga
     3421 attcgtgttt cagaagggag ccgaggataa atggaaatga taccaaagcg ttatgaaaca
     3481 gttgcagata tccctgcgga tgaatttctt ccattacaaa aaaataccga agaagaacgt
     3541 gaacagatta ctgcaccttc tttgaatttt atccaagatt catggcgccg tttaaagaaa
     3601 aacaaagctg ccgttatttc aatggtttta ctattggtca tcattttgat ctctgtgatc
     3661 acaatctttg tttctcctca tgatccaact gcccaaaacg tggcttatat caatttgcct
     3721 cctcgtattc cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa
     3781 cttgttgata aatatgctca agcaaacgtc ccttcaaacg taaatttctt tttaggaaca
     3841 gacggtttgg gacgtgatgt attaagccgc ttatttatgg gaatgagaat ctcgttgctg
     3901 atcgcattta ttgctgcatt actagatgta acgattggtg tagcttacgg gttggtttct
     3961 ggtttgttag gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat cttatcagga
     4021 attccaaacc tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg aattttttca
     4081 atcgtggctg ctatggccat cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa
     4141 acaatgaaat taaaagatca agaatatgtt ttagctggat tgacattagg tgaatcaaga
     4201 atgaaaattg cattcaaaca tattttgccg aatatctcta gtgtcattat tattcaaatg
     4261 atgtttagta ttccatctgc tattttcttc gaagcctttt taagctttat cggtttaggt
     4321 cttacgcctc cgtcagcttc attaggaacg atgttgagcg aagggtacaa gacattcctg
     4381 tatctgcctt atctattatg gattccggca gtaactttat cggttatcat gattggcttt
     4441 aacttacttg ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga
     4501 tgacgaaaat tttagaagtg aaagacttag aaatctcttt tgatacgtat gctggaaagg
     4561 tacgagcgat tcgcggtgtc aattttcatt tgaataaagg agaaacattg gcgatcgttg
     4621 gggaatctgg aagtggaaaa tcagtgacga ccagaagcat catgcgcttg ctgtcaagca
     4681 acgccaatat agatgctggt caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag
     4741 aaagagagat gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa gatccaatga
     4801 cctctttaga tccgacgatg cccattggga agcaagtcgc tgagtccctt agaaagcata
     4861 ataaagtttc aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa ttagttggaa
     4921 tacccgaagc tgagaaacgt atcaacagtt atccgcatca gttctcagga ggtcagcgtc
     4981 aacggatcgt gatcgcgatt gcactggtat gttatccgga aatactgatt gctgatgaac
     5041 ctacaacagc gcttgatgtg actattcaag cacagatttt ggagctgctg aaagaaattc
     5101 agacaaaaat cgatacatca attatcttta tcactcatga ccttggtgta gtagcgaatg
     5161 tcgctgatcg tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga acatcggaag
     5221 aaattttcta caacccgcaa catccctata cttggggact gcttggttca atgccgacat
     5281 tagatagtgc gaacgatcga ttgtatgcta ttcctggttc tccaccggat ttattgaacc
     5341 cgccaaaagg agacgcattc tatccaagaa atgaattcgc gatgaaaatc gatgcagaac
     5401 aagaaccacc attttttgaa ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc
     5461 cgcaggcacc caaagtgaca ccgccagaag aaatccaacg tcgctgggct atctttaaag
     5521 agaaacaaaa acatacttac ggtcaaggag gaattgctga tgccaaataa actacttgaa
     5581 gttaaaggat tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat
     5641 gacatcagct ttcacattta tgaaggagaa acattcggtt tagttggaga atctggaagc
     5701 ggaaaatcca ctactggccg tacgatcatt cgcttgaatg aacctactga tggtgagata
     5761 ttattcgacg gtcaagatat aacgaagatc aaagataaaa aaggtttgac gaaattccgg
     5821 catgatgttc agatgatctt tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc
     5881 agagatatca ttgccgaagg aattgatgtt aatggactag ctaaatcatc tgaagagcgt
     5941 gcaaaaaaag tcgatgatct attgagaact gttggattga atccaagcca cggtactcgt
     6001 tatcctcatg aattttcagg tgggcaacgc cagcgtattg gtattgcacg agcattggca
     6061 gtaaaaccgc gttttatcat ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa
     6121 gcccaagtgg tcaatttgtt gcaagatctc caaaaagaac atcaattgac ctatctattt
     6181 attgcacatg atttgtcgat ggttaaacat attagtgacc gaatcggtgt aatgcataac
     6241 ggcttgttat tagagatggg aacaagtgat gaaatctata atcacggtgt tcatccttat
     6301 acagaaagtt tgttatctgc tattcctcta ccagatccag accatgagag aaaacgcaga
     6361 agaatcaaat atcaacctga accagatgac ggacaagtac gtcaacttcg tgaaatcgca
     6421 ccagaacatt ttgtctatgc aactgagcag gaagtcccat attacgcaaa aaaactaaaa
     6481 cgacaaaagg aatctcttct ggttgctaac taggagggac atagatgcta aaagctttta
     6541 aattccgtat gtatccgaca gaagaacaaa aacagcaatt gattcgtact tttggatgtg
     6601 ctcgatttac ctataaccat ttgctgaaaa agcggcagaa aagttggcaa caaacaggag
     6661 tagccaattt ttcgctcaca cccgctacat taaaaaaaga atacccattc ttaaaagaag
     6721 tagacagtct tgctttagca aatgcacagc ttaatttgga tcgggctttt cgcaattatt
     6781 tcaagggacg tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg caatcttata
     6841 cgaccaacaa ccaaacccgt acgatctatc tggaaaatgg ctacttaaag ttaccaaaac
     6901 aaaaagaact aatcaagata aatcagcatc ggccagttga aggatcgatt cgctccgcta
     6961 cgatttctgc tcgatataat gaagaatttt atgtcgcact actttgtgat gtttcgccag
     7021 taaaaaaaga atctttagct aaatggatcg ggatcgccta tcatccgaag acattgatcc
     7081 aaacatctcg acctctagaa gtaactttac caaaatttca ccagacagaa gaaaagctgc
     7141 aacatgcaca gcggaaattg aatgtgaaag tccgctccgc tcaccataga aagatcaggt
     7201 tagatcaggc aagcaattat cagaaacaaa agagaaaagt aatggatctt tatctgaaac
     7261 aaaagaacca gcgagaagat tatcttgaac aattatctgg gaaattggtc aaacaatatg
     7321 actacttatt tgtagaatct tttccaaaag aagaggcaca tgctgatttt tctattcacg
     7381 actggcataa attaataaca aaattgcgtt ataagtccca atggtataat aaaaaatttc
     7441 ttttgatcaa taccgacgga gcagaagaaa gcaattccgt aagaaagagc caagtagtag
     7501 aaaaaatggg cagacattcg gttatcaagg gataaaaaat gaatgaaact gataggagcg
     7561 ttagggttag ccttgttaat aaaaagtctt aatgactttt gtttctagga atttttcttt
     7621 tgaagaaaag ctcactaatt atgacaaacc aataaaacaa tagcagaaaa agttcgtacg
     7681 agctttttct gctattgtta attttttatt cagaaagttt acgaatacat caaaattctg
     7741 aaacaaaaaa cgtaactcac aaagagttgt ttttgcgttt tttcttgaat gattaggtct
     7801 gaatcttttg ctcacaacct tattcatggt gccccggctt gagctgatag atttttgaag
     7861 gacggccgat tcctacagga cgttcaccga tctcatcgaa atattggagc atagctttct
     7921 taaaattgga atgatcgatc gttttataat cgatccctaa gaatttagca aatacttttc
     7981 gagcttccgt aatcgtgaaa tctttaccta aaacttgtag aacttgcggc tcgtgttcca
     8041 ttttattgac tacacggttg aatgctttca agatgatttg actatggtca aaagccaaag
     8101 tatctttgcc agaagactct ccagtgacta aatcaagcga gatagccact tctccgctag
     8161 ataaatggat cttgttacta tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat
     8221 ctccggcgat tagcggttct tctccaataa aggctaagta gctgactgtg acgacccagc
     8281 ctcgcggatc tcgattaggc gtactgaatg tatgcagttg ctcaatattt tcttttgaaa
     8341 taaccacacc agtttcttcc tttgtttctc ttaagacgct ttctccagtt gactcatcct
     8401 tttggacaaa gccaccaggc aatgcccatg aatttcgata aggatgccct tttctttgga
     8461 tcaacaaaac tttcagctga tcttcttctt tgttatagca catcagtacg atatcgacag
     8521 tcaaagaggg cttgtcatat tctggacgtt cctgtttttg ataccagtta agaaattctt
     8581 cttcacttgc tttatgttca taatataatt tttcttcttc tttagaagca aaacggttca
     8641 tctgaccacc tccattgaca gtaaaagtac tttttacctt attgtagcag actctgaaaa
     8701 aaaaccaagg atttacttga aaaatctagg ttatcattta aatatgaaaa ataaatggaa
     8761 gtatgagatt gaataaaaag aaaagcagat aaaacggcaa tactttatac cgctcttcac
     8821 gaatggcaaa gaagccacaa ataacgatat atacgtcaat gataaaacgc aaaatccaag
     8881 ctgctgtcat aaaactgttc aaatgcccat ggaggttaaa ataaccatac atacgaatat
     8941 aaaaatagct gaatattaaa tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa
     9001 gatgtttctt ctgcatgatt gttccagtcc cttactaaga tagatagtct ttattatacc
     9061 acgtgtgagg ggagaaataa aaagccgttt tttagtagat atgaaacaaa acaaaaaccc
     9121 gcatttattc aacctattca tccttttaaa ggatagattg aacgtagata gaagtcgttt
     9181 atccagtctt tttcattttg aaaaaaagct aacagcatgc tttttttatg gtatactagc
     9241 aaggaaacga attcgcatca tgcgataaag gaggaagagc cccgtggcag aaaaggaaac
     9301 tttttatatc actacgccaa tctattatcc aagcggcaaa cttcatatcg gcaactcgta
     9361 tactacgatt gcatgtgacg caattgctcg ttataaacgg ttgatgggct ttgatgtgtt
     9421 ttatctcact ggagtggatg aacatggtca aaaaatcgaa acaaaagcag aagaactagg
     9481 tgtaaaaccg caagaatatg tagataaaat ggcggctgat gtcaagaaat tatggaaaac
     9541 gcttgatatc agttatgata aattcattcg cacgacagat gattaccata aagcagctgt
     9601 tcaaaaaata tttgatcgtt tattagaaca gggagatatt tacttaggtg aatatgaagg
     9661 ctggtactct gtttctgacg aagaattctt tacagaaaca caacttgcag aagtgtacaa
     9721 agatgacgaa ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag tagaattggt
     9781 aaaagaagaa tcatacttct tccgcatgag caaatacgca gaccgtctat tggaatacta
     9841 tgaagaacat cccgaattca tccagccaga atctcgtaaa aacgaaatga tcaacaactt
     9901 tatcaaacca ggattagagg atcttgcagt ttcacgaaca acttttactt ggggagtacc
     9961 agtcaagagt gatccgaaac atgtggtgta tgtatggatc gatgcattat ccaattatat
    10021 tactgcacta ggatatggct ccgaagatga aagtctattc gaaaaatact ggcctgcaga
    10081 tgttcagatg gtcggtaaag aaatcgttcg ctttcatacg atctattggc caatcatgct
    10141 gatggccctt gatttgcctc tacctaaaaa agtatttggg catggctggc tattgatgaa
    10201 agacggaaaa atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg
    10261 ttatggttta gatgcactcc gttattattt gcttcgtgct gttccgtttg ggtcagatgg
    10321 tgtctttacg ccagaagatt ttgtttcccg tgtcaattat gatctggcaa atgatctagg
    10381 gaacttattg aatcgaacga ttgctatgat caacaaatat tgtgatggac tcgttcctga
    10441 ctatgcctca cttgtcactc catttgatag tgaactgtct acgacggctg ctaacgttgt
    10501 cggacgttac catgatgcaa tggaaaaaat ggaattcaat acagcattgg ctgaaatctg
    10561 ggtgctgatt tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg tattagcaaa
    10621 agacgaagaa aagaaagctg aattgaatag tgtcatgatc catttagctg aatctttgcg
    10681 tatcgcagcg atattacttc agccaatcat gacagaaacg ccagagaaga tcttcaatca
    10741 actaggattg gactctgaaa caatgaatct tgaaggatta cactttggcg aatttccttc
    10801 aggaacaaaa gtagttgcaa aaggtacacc aatcttccca cgtttagata tggaagaaga
    10861 agttgctttt attcaagaaa aaatgtctga aggtactcaa acaaacgaag atacagtcaa
    10921 atgggatcca gaagaaacag aacttgtttc aacgaaagaa aaacaaatca aatttgatgt
    10981 atttgaaaaa gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc
    11041 agataagttg ctgcaattcc gactagatgc tggagacagc caagaccgtc aaatcttgtc
    11101 aggaatcgct gaattttatc ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt
    11161 agcaaatctg aaaccacgaa agatgcgtgg acaaatcagt caaggaatga tcctttctgc
    11221 agaagcacca gatggctctt tacaagtaat cgaagcacca aaatccatgc caaatggctc
    11281 agaaatcgca tagaaattca aagggggtat gacactagtt gtgtcatacc ccctttttcc
    11341 gaaatttgag aagcaatttt cgtaaattcc ttcttattac ttgaagccaa tcatataata
    11401 cagaaacaga gaactatcca aaataaatcc tagaaaaaga agtacgagct tcgctgattt
    11461 ttgttaattt agaataatac taataggaat cgtaaaaaca gaaaaaaagc ataatgataa
    11521 agggtcgggc agattttaat tgattcatct ggtactcttt tttattgtcc ttacattcat
    11581 aaagtttgaa tgcaatgact ttattacatc tatatttata tctaacagta gcgttgatgt
    11641 gttaattata agttctatca ctttatgttc aattagtttg tgctcttata tgtgtgattg
    11701 aaaatataga taaaaaaaca atcatcggct ttgacttcga tgattgtttt ctttattttg
    11761 taaaaatcct atcacttgat tttatggtaa ccttttattt ttgtcttcaa acggcttggt
    11821 aagagagcag taagcagtgc aaatgggatc gaaaagacat accagaaaat cgtacgtccg
    11881 ataccataat gttgatagag ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc
    11941 aaggagccag ctataaacca atgattatcc caatgcatga cttcactaag tgccatcact
    12001 ccaaaaggat agaaataaaa atagatcccg actaaggtca tccgccagtt ctttgtaaat
    12061 gttccgatat tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc
    12121 ccaaccaaaa aggaatacaa catcccccac aagatcatca caggaccttt gatcaatgca
    12181 acgattgcga tgaacaaagt cagcaatatt agtttttgtg ataacgttgc aacagtcatt
    12241 aatccaaata aaattaatgt gaaccaatgc caatgggact gctgttgttc tttacgggaa
    12301 aaatcgaccc ggttggataa ataatctgct aatttcggat aagaacgctt aaaatccata
    12361 aactgttcct ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa aagaaataca
    12421 tccgtcttaa gagggagttt ttcactgaaa gtggagaaaa aagaatggta aattaaaata
    12481 ttctcagtta tactgaaaat tataccaaat ttcaaagaag aaagaggatg ggaatgaaag
    12541 gcaaactaaa acgagaaatc aatctgtttg gcgcattagc taccgttatg ggaaccgtga
    12601 tcggtgcagg cgtcttcttc aaaactgctg ctgttactgc tagtactcag tctacaagtt
    12661 tgacattgct agcttggctt ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg
    12721 ctgaattagc tacagcaatc ccggaaactg gtggagctgt aaaatatatt gaagctgcgt
    12781 atggaaaatt gcccagcttt ttattaggat gggcgcaaag cttgatttac tttcctgcta
    12841 atattgctgc attatcgatt atctttgcga cacaattgac gaacttatta caattatcta
    12901 ccgattatct tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt
    12961 tagggacaaa agtcggtgct tctgtacaat cagtgacgtt gattgttaaa ttaataccaa
    13021 ttgctgtgat tgtcatatgg ggattattga ccccaggaca aggaactgtc cagttatttc
    13081 caatcgaagc tggaaaagat gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa
    13141 cgttgtttgc ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc
    13201 cggaaaaaga tttgcctaaa gcaattattc ttggtttgag ttttgtaaca gtcgtttatc
    13261 tattgatcaa ttttgttttt ttgaaaactt tgccgattga tcatcttgcc ggtaatttga
    13321 atgcagcttc tgaagcatca gacgtcattt ttggaggaat cggtggaaag ctggtcacta
    13381 ttgggatctt gatctcggta tacggtgcat tgaacggtta tacactaact gggatccgcg
    13441 tgccttatgc gatggcttta gaagacgagc tacctttcag taaacagctg acaaagcttt
    13501 caaagaagtt tacggttcct tatgttcctg cggttttcca gttagcagta gcttgtatta
    13561 tgatgagcct tggttctttc gactttttaa cagacatgtt gatttttgtg atgtggctat
    13621 tcagtttact gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac
    13681 gtccatataa agtaccactt tatccaatcg ttccgattat tgcgattctc ggtgctattt
    13741 tcattttagg tatgacgatg atgacgcaaa cgaaattagc attgatcggt ataggtgtta
    13801 ccttgatagg tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata
    13861 tttcaacaac agacagaaaa agtacgaact aggtcctcta gatcttcgtg ctttttctat
    13921 ttcaataaaa caagttcttc tattttgatt tttgctactt ctgcaagtag tgcttatgtg
    13981 ctaaagtaag aatgaaaagg agagataaac atgatatttg attctcatac ccatttaaat
    14041 gctgaacagt tcaatgaaga tattccagaa acgattcagc gagcacaaga aatgggggta
    14101 acgaaaatgg cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt gcaattaagc
    14161 catgactatc caaatatata cagtattatc ggctggcatc cgacagaggc tggcagttat
    14221 acgaaagaaa tcgaaaataa attacaagaa caattgactt taccaaaggt tgtagcatta
    14281 ggggaaattg gcttggatta tcattggatg gaagacccga aagaagtcca agataaagta
    14341 tttcgcagac agattgctat cgcaaaagaa atgaacctac caatcagtat tcatactaga
    14401 gaagcacttg aagacacgta tcaaatccta aaagaagaag atgttcgaga tatcggtgga
    14461 atcatgcata gtttcagtgg tgattatgaa tgggcaaaac gatttcttga cttagggatg
    14521 catatttcat ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca agaggctgca
    14581 gcaaatgtac caatcgaacg attacttgtg gaaacagatg ccccttatct ggcaccagtt
    14641 ccttttagag gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga aaagattgct
    14701 gaacttcgga atcttccgac agaagaagtg gctgcccaaa catggacaaa tgcgcaccga
    14761 ttatttggtc taagcgaaca tggataagat gaagatccaa gagattatag tcgttgaagg
    14821 aaaagacgat acaagaaggc tgcgggaagt agttgatgca gatacgatag aaacaatagg
    14881 gtctgcaatc aatgaagaga tcctgatgca aattgaacat gcccaagaga ctcgtggagt
    14941 gatcgttttt accgatcctg atttttcagg agaaaaaata agaaaaatca ttatggaagc
    15001 tgttcctcag gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg
    15061 aagccttggt gtcgagcatg caagtgatgc tgctatttta gaagcattga aaaaggtcgt
    15121 gacgccagac atttcccaaa cggctgtttc tgaaatcact agacaagacc ttttagacta
    15181 tggtttaata gctggggcac aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg
    15241 catcggttac acgaacggca aacaattgga aaaacgtttg aagatgtttc gtattacgcc
    15301 gaaagaactc gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa
    15361 ttgcgacacc ctctagaaca aaagagattt tattgaaaca tggtttttca tttaaaaaaa
    15421 gtttaggaca aaattttctg actgaaccaa atattttaag gaaaatcgta gaaactgcgc
    15481 aaatagatga acatacgaac gtgatcgaag ttggaccagg aatcggtgca ttgacggaac
    15541 aattagccaa gcatgcaaaa caagtcgtag catttgagat cgatgatcgt ttgatccctg
    15601 tattggctga tacgatgcaa ccctatgaca atgtgactat tgtacatcaa gatattttaa
    15661 agaccgattt gtcaacagct gttagagaaa catttcatga ggagcttccg ttaaaagtcg
    15721 tagcgaattt accttactat attacgacac cgatcatgat gcattttctt gaatcagatt
    15781 tggttgtgga tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt atctctgctg
    15841 aaccaggaac gaaagcttat ggttcattat cgattgctgt tcaatattat atggaagcca
    15901 gtttagcatt catcgtacca aaaacagtat ttgtccctca gccaaatgta gattcagcga
    15961 ttttgaaact gacaagaaga gatataccgg cagtcgaagt gacagacgaa aaagcatttt
    16021 tccgattgac aaaagcggca tttcagcaaa gaagaaaaac gctttggaat aacttgcagc
    16081 attcgtatgg caaagatgac cagacaaaag catggttagc aaaaagcttg gaaacagctg
    16141 gaatcgatcc aaaacgtcgt ggggagactt tgtcattaca agaattcgcc gcattaagca
    16201 atgcgatgtc agaaaatatg cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct
    16261 gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt
    16321 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgggcc
    16381 tgccgtttaa ctctaagtca caaggaacaa tttcaaaaaa cagcttctca tgtttttgag
    16441 ttagttggtc ttattacttg atgcaagctt ttgaccacta ttatgccgaa caaggagatg
    16501 tgacacaaat tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg
    16561 atactcacac tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta
    16621 agtgagttat actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga
    16681 gaaaaaattc agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta
    16741 tcccaatggt catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg
    16801 tgccattatt tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta
    16861 cgacatttgg tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc
    16921 ttgtgtttgc gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa
    16981 atggacagtt tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga
    17041 ttcattcttt agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg
    17101 aaacatatta ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc
    17161 ttatccacag tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat
    17221 attttgatat ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag
    17281 ggtacgcgaa gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt
    17341 aatggataat aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat
    17401 cttctggcac agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct
    17461 ttatcaaaga agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta
    17521 tggaaatctc ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc
    17581 atcgtttcag ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt
    17641 gaagcttcaa gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact
    17701 tcttttcctt cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc
    17761 ttaagcccat gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg
    17821 gcaggagatc caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc
    17881 ggtgtaccga tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc
    17941 attgcttctg tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt
    18001 ttggcaatct cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca
    18061 tccattaaga agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt
    18121 tgtccaccag ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct
    18181 gctgcattgt ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca
    18241 aaagccatat tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg
    18301 gcaatatcac ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct
    18361 ccttcagaga tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg
    18421 gaaggaccaa caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca
    18481 gaataatttt ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata
    18541 atacgttcca cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt
    18601 aactaacttc aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt
    18661 ttaagcttta ataaatatcc ataagaaata aaaactgata cactggagta aataagtatg
    18721 ttaacaatat caaaaggagt gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa
    18781 aaacctatga tggtgaaatt agcaacagcg tataaaacaa tattggaaaa acatgaactt
    18841 tttgctaccg ggacgactgg gcagaggatc acggaagcaa ccggcttgcc gatccatcgg
    18901 tttaaatccg gaccgttagg cggggatcag caaattggag ccatgatttc ggaggatcag
    18961 ttagatttgg tgatttttct tcgagacccg ctggctgcac agccccatga accagatgta
    19021 acagcactta tccgtttatc tgatgtctat gagattccgt tagctacgaa tatcggaaca
    19081 gctgaggtgc tactaagagg actggaagcc ggcttcctag attttcgaag aatcgtccat
    19141 gaaatagaca agcatccact ttcattttga tggataagcg tatggaaaaa taacgtttaa
    19201 tgttcgccta tatgtagcta taaaagctaa acaaactgat aatatgcgat ataatacgaa
    19261 cgtattggat aatataaaca ataatattag tttttcgtat ttgcagtaaa tgtgaaaata
    19321 tgctatagtt tctgtgggaa aagatcccaa aaataataca gagtagaaaa taaagcgtta
    19381 agtgctaaga ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg cggttttatt
    19441 ttcgcattcg ctgcatgata cgatgtagca gctcttaaag gagatgctaa aaatgaagaa
    19501 acaccggtta gacgcacgta tgattgctat tatgggctta ttgattgcat tgatggtcac
    19561 tttgtcccga ctggtagcaa tcgagacccc atttattaaa atcagtgtca catttattcc
    19621 acaagtgatc atgggcattc tttttggtcc tttttggtca gggataggcg cagttttagc
    19681 tgatttagta ggaatggccc tcttctcgaa atctgcattc tttattggat ttacattgaa
    19741 tgcatttatt gaaggagcaa tctacggatt ctttttctat cgaaaagaga tcacttggaa
    19801 aaatgccatt ctggctacgc ttagtgtcac gctgatcatc aacttatttt tgacaccatt
    19861 atggcttgcg ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg cgccccgcct
    19921 gttaaaaaca gtaatatggt taccgattca atcaattgcc atttattacg taggacgtag
    19981 tattccttac aaaaaaattt taagaagttt agcgatccac gctaaataaa aaacaagcag
    20041 tcttcccatc ccagaagact gcttgttttt attgtgctgt gaattgtgct tgatagagcc
    20101 gtcggtaata accatctttt tgaagcagct catcatgggt gcccatttcg acaatagctc
    20161 cctgatccat cactaatatc tggtccgcat ttttgattgt ggacaggcga tgggcaatca
    20221 caaaactggt ttttccttcc atcattgcta agaaggcttg ttggattttt ttctctgtca
    20281 atgtatcgac tgagctggta gcttcatcta agatcaacat agggggatta ctgatcatcg
    20341 ttcgagcaat cgtcaacagt tggcgttgtc catctgatat ctttaaccct tgaccgccaa
    20401 tcttagtatc taatttttcc ggcaaacgtt ctacaaattc atacatataa gaggctttta
    20461 gggcatcatt gatttctgcg tcacttgctt ccggattccc aaaacgaaga ttttctcgca
    20521 atgtactgtc aaatagccaa gtatcttgta acaccattcc aaaactttta cgtaatgtat
    20581 cacgttggat agagcggatg tcatggtcat cgactgtaat tgttcctttt gttgtgtcat
    20641 aaaaacgcat cagcaaatta acaagtgtgg attttccggc ccctgttttt cctactatag
    20701 caatggtttg acctggataa gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt
    20761 cataagaaaa agaaacatga tcaaaagaga cttttccttt gatatttttg ggttccaagg
    20821 cataggcatc tggcaaatca ggcttttctt ctggctgatc gatcaaatca aatgctcgtt
    20881 ctagaccaga aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt
    20941 tagaaaactg acttgagtag atcgtaaagc tggaaatcac accaacagtg attgacgaac
    21001 cactcttcaa tgccaatagg ccgccaacta aaccaatcga taaataagca aggtgatcta
    21061 caaaacgaga agacgggttt gtcaaggaag aagaaaattg tgcccgttgt cctttttcat
    21121 atagatcagc atttagcagt tcaaattgct gttgactagc ttcttcttgc tgaaaagctt
    21181 tcacgatttt ctggttaccg attcgttcgg tcacaaagcc cgagatatcc ccaataatcc
    21241 tttgctgtct atcgaaattt gtttgtgaag cacgagcaac gatccaactc acaaggaaaa
    21301 taattggtgt agaacaaaga acgaccaatg tcagaagtgg acttagacgc atcataaaaa
    21361 taaacgaaag aatgatgatt gccactccag aaaataattg attgaaggcc gcagaaattg
    21421 caatggaaat attgtccata tcatttgtaa atcgactgac gatatttccg tgggagtttt
    21481 gatcataata acgaagtggc aatcgattca gatgagcaaa tgcatctttt cttaattttg
    21541 caacggataa gtaagctaac cgattgctta acacttggat gagccattga ctgactacgt
    21601 tgattaaaac gataccaccg aatagaataa gtaaatggaa cagtcgattg aagcggacct
    21661 gtccttctcc aatcatttga tcgatacttt gaccaatttg taacgtcatc acaactgttg
    21721 caatcccatt gatgattcct agtatcacag ctagccaaat gtcttttgca taggcagtaa
    21781 ggtaaggaat gaatcggcgg agcgtctgca aggataattt ttttactttc ataactattc
    21841 ttcctcctgt gatgcgacga tttcctgata ttctgctgat tgagcaagta attcttcatg
    21901 ggtaccctga gcagcaatct taccttgatc catcactaag atatgatgtg cttcttgaat
    21961 agagcggaca cgttgggaga taataatcac tgtactgcca agataagtct gtagcgcctt
    22021 acgcaaatcc aagtctgttt gataatccaa cgcacttaaa gagtcatcta aaatcagtaa
    22081 tgctggcttg gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa
    22141 atttttccca ttttccatga cttttgtatc taaaccattt ggaagctgac ggacaaattc
    22201 ttctgcctga gcgattttca gagcttccca acattctttt tctgtggcgt ctttcttacc
    22261 ccattgaagg ttttcacgaa tagtcccggt aaatagtaca gaagtttggg gaacaagagc
    22321 aatttgctga cggagatgat ccagctgcca agaacgaaca tccattccgt ccattataac
    22381 tgtaccggaa ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct
    22441 tccggttgga ccaatgattc caaggactgt atttcgcttc agctgaaatg taatattttg
    22501 gagagaaaga ccagaatcag gagtataacg gaagtcaaca tggtcaaaag cgataattgc
    22561 ttcagaagaa aaatcgggag tagtactgac ctcagcttca agaatcgaat tttctgtgtc
    22621 tagcacctct ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt
    22681 tgaaacaacg atcagtgcca aaagcatttg attcatataa ttgatcaaag caagaacttg
    22741 tccttgttgc aagctaccaa tatttacttt gatcccgcca actgtcagca aacagatgat
    22801 cccgacattc atgatcaagg ttgtagcagg tgataatagg gcagagatat tggaaacacg
    22861 taaataattg ttcgctagat catttgtgct tttatcgaac ttctctgttt ctttttttgt
    22921 ctgcgcaaat gcgcggatga cacgaacacc gctaagattc tgactaacgt gttcattgag
    22981 ctgatctagt ttttcttgca ctttttggta gagaggaacg gtttttttga tgataaaaaa
    23041 taaaatgatt gagaaaatag gcaataatgc taagaaaaac agacctactt cccagtcgat
    23101 cacaaaagcc atgaccactg acccgatact taaaaacggt gctctgatca ataaacgaat
    23161 gaccattgct aaagctagct gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt
    23221 atctgttcca aaatgattca actcgttgtg agagaaagta ttgatttttt tgattagctg
    23281 attacgtaac tctgtaccaa atccttgtga agctactgaa gcatagtatt ggcaaatgat
    23341 agcacaaatc agaccaatca cagacatagc gaccatccat aagcccattt caatgatttt
    23401 tcctcggtca ttcattttta gtccattatc gatcaaggaa gccatcatta aaggcaggac
    23461 aagttcgaag acagcttcaa gaaatttgaa gaacgggccc aaaatgattt gcttacgata
    23521 gtcttttgca taacgtaata actgaatcat atgattcctc ctattattta tttgtccaca
    23581 attgaaaaaa caagtactac cacattgtag cttaggaaat ttagaatgaa aagagaacaa
    23641 gccttttcat aaatgaaagt tttattatga agaagcttta gaaattgaat agactatttt
    23701 ttttaaaaaa aagaaaatga gaaaacatgc cagtctcttc attcatagga acgtctattt
    23761 tacttaattt ttctataaat gaattctgca taaaaaatat atcaagtaaa tgtttataga
    23821 tgaaactcta tttacttctt ttttaatttg ttcttgcaat cttttttgct acgtaatttt
    23881 tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa agaaggagta tgaaaatgag
    23941 aaaaatattt attcgtttat tgtgggtact atcttagagg aataaataat agtggagaaa
    24001 actatggttt ggcgcagatt ctatcaggtt tgatggaacg aagacaacct cccgacatca
    24061 agtcaaaagc tacttgagat tttgatctga aaataaaaaa agaaaacaag gtaggaacat
    24121 caatgtttct accttgtttt cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc
    24181 aataaaaagg atggtgacaa cagtacctct ttactctctg aaagcatcat aactcgtttt
    24241 tcctcattat gccaataaat tgttgttata ccacatataa ggatttgtaa tagctaaaaa
    24301 cagacaggaa agttgaaatc tgtgaatctt attttgtatg gaaggattta tagaacgcaa
    24361 aaatcaagat aaagatgaat aaaaatcctg tttcggctat atattgcgta tctacgccaa
    24421 taaaacgagt ttgttctgtc atagccatat aaagcaaagg caaaaataca actacagcga
    24481 gataagatcc gatgatttct cgaaaagttt tccaggaagt atgtttcctg tttttatatt
    24541 tatccgattt cattgaatag tcatagcctc cttattcttt catatgttga aattccagta
    24601 taaaaactgg aaggaactgt acgtttttta gaataaaaat tataagggat aagtaggctg
    24661 ctgtctataa taagatatat ttttcaattc tttattaaag attttattga caaaatgaat
    24721 ccgttttcta tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact
    24781 tagagaacca gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa
    24841 ggttagctga tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa
    24901 atgatgagca actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag
    24961 cttttgaaaa acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca
    25021 aagaggaaca gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg
    25081 acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg
    25141 gattaccact tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg
    25201 tctatcagta ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta
    25261 attgtgcaca atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta
    25321 agggagcgta cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg
    25381 ggatcttgtt tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca
    25441 cctatcttga tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca
    25501 aaacacgtct cttttttgct acgactgaag agattactag tcatttctta acaactttca
    25561 ttcgaagaat acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac
    25621 ggctagaact gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt
    25681 cagtcagtgg accagctttg tcacttttaa caagtcagaa attatccgga aacataggag
    25741 agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc
    25801 ctgaaatcca tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta
    25861 aaaatacagc acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg
    25921 agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt
    25981 tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc
    26041 aattatttga ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt
    26101 attggacaca atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat
    26161 tcgatggaaa cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat
    26221 ggttaccgga agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc
    26281 agtcttatcc atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat
    26341 tagatattga agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga
    26401 aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg
    26461 ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg
    26521 atatgccact agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa
    26581 acagcgatgt ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc
    26641 atcagttttt taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac
    26701 ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag
    26761 aagagactgt tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac
    26821 ccaaagcact cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac
    26881 tgctagaaaa aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc
    26941 aattaaaaga taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga
    27001 ttggcaccac agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct
    27061 cgggggaaaa gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga
    27121 aggaaatatt caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg
    27181 tgactattct agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag
    27241 aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga
    27301 gctgtctcat tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga
    27361 aatggaagca gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg
    27421 aagagcatta tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga
    27481 gtgaagatac agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc
    27541 actaattttg gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa
    27601 agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc
    27661 attagagctg atctatggga aaaatcattc tattactgta ttcgattgct atactgatga
    27721 gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat
    27781 cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa
    27841 tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc
    27901 tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa
    27961 aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta
    28021 ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta
    28081 gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta
    28141 cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta
    28201 gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat
    28261 aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca
    28321 gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga
    28381 aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa
    28441 atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg
    28501 taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt
    28561 cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct
    28621 gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga
    28681 gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg
    28741 tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt
    28801 actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt
    28861 gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt
    28921 cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat
    28981 ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt
    29041 cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct
    29101 tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc
    29161 ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt
    29221 ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga
    29281 agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg
    29341 tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg
    29401 cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg
    29461 cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga
    29521 ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga
    29581 ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt
    29641 tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag
    29701 gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc
    29761 gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg
    29821 caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg
    29881 gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa
    29941 atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag
    30001 catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg
    30061 gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag
    30121 aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg
    30181 aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta
    30241 gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt
    30301 gatcttactg tcgatattca agaacctttt tattttttca attatggaaa actttctcct
    30361 tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca
    30421 gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt
    30481 ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc
    30541 ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt
    30601 ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc
    30661 aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat
    30721 caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga
    30781 acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt
    30841 gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc
    30901 tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt
    30961 cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt
    31021 ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa
    31081 ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa
    31141 gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat
    31201 gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa
    31261 atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga
    31321 acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc
    31381 tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac aaaagattct
    31441 atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa
    31501 atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct
    31561 cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt
    31621 atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat
    31681 gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca
    31741 tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca
    31801 gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg
    31861 cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa
    31921 gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt
    31981 gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat
    32041 ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat
    32101 gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata
    32161 cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat
    32221 aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta
    32281 taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg
    32341 ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta
    32401 aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac
    32461 taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga
    32521 cagatttaga aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa
    32581 gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag
    32641 gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga
    32701 ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt
    32761 cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat
    32821 ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa
    32881 cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg
    32941 aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga
    33001 ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg
    33061 ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc
    33121 caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag
    33181 ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta
    33241 gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc
    33301 aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa
    33361 ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg
    33421 ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag
    33481 gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg
    33541 gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc
    33601 gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa
    33661 tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg
    33721 ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa
    33781 aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt
    33841 aaatagatga tcaatagtta atcgtcgttt aggaggaaca gcaagtgaag gtcaaaaaag
    33901 cagtcattcc tgccgctggg ctaggaacac gttttttgcc ggcaacaaaa gccatggcaa
    33961 aagaaatgct tccaatcgtg gataaaccaa cgatccagtt tattgtggaa gaagcattgg
    34021 attcgggaat cgaagacatc ttgatcgtaa cgggaaaaga aaaaagaccc attgaagatc
    34081 actttgatgc aaatatcgaa ttggaaaaca atttgcggga aaaggaaaaa acagaattac
    34141 tcgctttagt tgaagaaacg actcaagtca atcttcactt catccgtcaa tctcatccaa
    34201 gaggactggg ggatgccgtg cttcaagcaa aagcatttat aggaaatgaa ccgtttgtcg
    34261 tcatgctggg ggatgatttg atgaaagatg atatcccatt gactcaacaa ttgatgatgg
    34321 attatgaaga aacacaagct tccgctgttg ccgtcatgcg tgtaccagaa aatgaaacat
    34381 ctaaatacgg aattattgat cctaaaacag atagcgaaaa gggtcgttgc agagtaaaag
    34441 ggtttgtaga aaaacctgaa atgggaaaag caccaagtaa tttagccatt atcggacgtt
    34501 atctactgac tcctaaaata tttgagatat tagaaactca agaaccagga gcaggaaacg
    34561 agatacagct aacggatgca cttcagactc ttaaccagac agaggcggtc tatgcgagag
    34621 agtttaaagg aaaaagatac gacgtaggcg ataagttagg atatatgaag acaaatattg
    34681 aatatggact tcaacattcg gaaattggga cttcattaag tgcatatatt atccagttga
    34741 gtaaaaagct aagaacagaa agttaactgg aaataagtgg cctgctgaga atgaaaggct
    34801 atttgttatt aaagcggcag cttgtgcgtc atttgcgcaa ctgtcgtttt agttttttga
    34861 ctctagatca gttgagtcgt ggaaaacgaa agatagaaaa agactttcgt gaatagattc
    34921 agaaaatatt atgctaatgg tttaattttt ttttaagttg tataaaagaa aatgattaca
    34981 taagagaaat gaaagccaag ccagaagatt catagtagaa taaaaaggcg aattttttaa
    35041 ttataaaaaa aggtgtgtgt tcactatggg aaaattagtc gctgttgcac agtcgctgct
    35101 tgatatactc aagtggctga tggaaggaag caattggata tttgtgttgc cactattgct
    35161 tattagtcta tttatcatgt ttcgcatggt cagaaaaaaa gaaacgtttc tgatttttat
    35221 ctggtatgca gcatccgttg gggttgctac cttgatggtt gaattggcgt taggtagttg
    35281 ggaagcttgc gtatttgctt tattttttgc tgtactgtat ctaatcattt atatcccata
    35341 tatgaaaaga aagtctcgga taggcagaaa aggtagacat acaactactt tttctagtac
    35401 agattctctc gaacaaagta gaagaacgag aagaagacgg taatatttag taggtatacg
    35461 caaaaaaaga taaaataatg gataaactat tgagatgaga gatttattta attggtggaa
    35521 aaaagctttt ttcacttata taaaagaatc ttttttaaag gaatgcttgt tgtatcggga
    35581 taagccattc ctttttttat gttatttata tatatataaa tcataccaat gtttttttgt
    35641 attttcttgc ttgcacttgg ataagagatt acttacaata aattcagata gaaacagctg
    35701 aataaaaaaa ggctccctga cccttagttg gcgctaagaa tcagggaatg accagtcaca
    35761 cacatacacc ctgaccgatc attttgccat agtcaatgca gtgcatcgac ctaatttatt
    35821 gtacctaatc tagggtgaaa attctacccc tatgtagata ttttccatgg tctgcacgtt
    35881 gttttatttg ctatgtcaag gtattgggcg tattcgttca atgccttttt tgtgtttgaa
    35941 acagcaaaga ccacatgtgt tactgtctaa ctactgtttt tttatgtact tccttagatt
    36001 aggcagctgt ttctattcat gtcaagtata gctaggcggg agagataaat gagtttatgt
    36061 tattttgact attatcacca attggaagag aaattaagga tagaagagcc gctgatgctt
    36121 caagctggca cttggatcgc gaaacaaatt aatcaaggtg gtagattgca gatatatgct
    36181 agccgcacac taagtggtgt agcatttgaa ttttgggatc aaaccccgga tcttgtacca
    36241 agtatattga ttgaaaatcc agccggaggt gtctatgaaa cgctagaagg tgcaggacag
    36301 gcaattattg atcagctttc tgtcaaatca gaagacattt ttcttcttct ttcgaatgaa
    36361 ggaagaaacc cggcaatcat cgaactagct cagtggatca aagaaaatgg tcatccatta
    36421 atcattgtta ctggatttga tttgtcacga agtatcaaat cgcaacatag cagtggtttg
    36481 cgattatttg aatttgctga tttggttttg gacaaccacg ccaatttaca tgatgcagtc
    36541 ttgactttgc caaatctaga tccagcgatc tgcggaacag cctctcttgc aacgatcttt
    36601 ttgctgcagc aagttttgta ttttacagca aaacagctag tccaggacac aagtggaaaa
    36661 tagttatcca catgttgaca aagttcctta aaaaaataat gttccacata aaaatggagc
    36721 gtttgaaaaa aagaaaacga ttattaatga ggattaacaa cgactacttt ttttgtttta
    36781 gttattttaa gatggatcat ctttgatttt aagcttgttt tgtttaaagt ttgttatttt
    36841 tataagttgt ttcacagtta tgagaaatga gttataagaa tatgtagtgt cagaaaaata
    36901 aaaagtagtg gaaaaagaga caagaaagaa tccatatgga ttctttcttg tctcttaata
    36961 cacgttgatg aaagagtctt ccccaaaact ctttacaaag taattatagt acatttgtta
    37021 tcattattgc aatgaagttt acctgatata acaaaattgt ccaacaggat acctttttct
    37081 aatagaaata aaatacacaa aaa
//
LOCUS       DADQRX010000016        35513 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.016, whole genome shotgun sequence.
ACCESSION   DADQRX010000016 DADQRX010000000
VERSION     DADQRX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.016"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            220..537
                     /locus_tag="J8V14_000775"
     CDS             220..537
                     /locus_tag="J8V14_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920901.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            470..949
                     /locus_tag="J8V14_000776"
     CDS             470..949
                     /locus_tag="J8V14_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBA0920902.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1399..1830
                     /locus_tag="J8V14_000777"
     CDS             1399..1830
                     /locus_tag="J8V14_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920903.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            1827..2153
                     /locus_tag="J8V14_000778"
     CDS             1827..2153
                     /locus_tag="J8V14_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBA0920904.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            2272..2958
                     /locus_tag="J8V14_000779"
     CDS             2272..2958
                     /locus_tag="J8V14_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0920905.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            2933..4282
                     /locus_tag="J8V14_000780"
     CDS             2933..4282
                     /locus_tag="J8V14_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA0920906.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4273..4746
                     /locus_tag="J8V14_000781"
     CDS             4273..4746
                     /locus_tag="J8V14_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0920907.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            4748..5248
                     /locus_tag="J8V14_000782"
     CDS             4748..5248
                     /locus_tag="J8V14_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBA0920908.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5263..7575
                     /locus_tag="J8V14_000783"
     CDS             5263..7575
                     /locus_tag="J8V14_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBA0920909.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            7822..8139
                     /locus_tag="J8V14_000784"
     CDS             7822..8139
                     /locus_tag="J8V14_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBA0920910.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            8136..8297
                     /locus_tag="J8V14_000785"
     CDS             8136..8297
                     /locus_tag="J8V14_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBA0920911.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8294..8599
                     /locus_tag="J8V14_000786"
     CDS             8294..8599
                     /locus_tag="J8V14_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920912.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            8599..8913
                     /locus_tag="J8V14_000787"
     CDS             8599..8913
                     /locus_tag="J8V14_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920913.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            8906..9436
                     /locus_tag="J8V14_000788"
     CDS             8906..9436
                     /locus_tag="J8V14_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBA0920914.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9433..9702
                     /locus_tag="J8V14_000789"
     CDS             9433..9702
                     /locus_tag="J8V14_000789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920915.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            9832..10119
                     /locus_tag="J8V14_000790"
     CDS             9832..10119
                     /locus_tag="J8V14_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920916.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            10606..11019
                     /locus_tag="J8V14_000791"
     CDS             10606..11019
                     /locus_tag="J8V14_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBA0920917.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            12215..12760
                     /locus_tag="J8V14_000792"
     CDS             12215..12760
                     /locus_tag="J8V14_000792"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0920918.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            12761..13078
                     /locus_tag="J8V14_000793"
     CDS             12761..13078
                     /locus_tag="J8V14_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA0920919.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(13235..13399)
                     /locus_tag="J8V14_000794"
     CDS             complement(13235..13399)
                     /locus_tag="J8V14_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBA0920920.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            13501..14022
                     /locus_tag="J8V14_000795"
     CDS             13501..14022
                     /locus_tag="J8V14_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBA0920921.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            13994..14449
                     /locus_tag="J8V14_000796"
                     /pseudo
     CDS             13994..14449
                     /locus_tag="J8V14_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(14489..14647)
                     /locus_tag="J8V14_000797"
     CDS             complement(14489..14647)
                     /locus_tag="J8V14_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBA0920922.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            14740..15501
                     /locus_tag="J8V14_000798"
     CDS             14740..15501
                     /locus_tag="J8V14_000798"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBA0920923.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            15688..17040
                     /locus_tag="J8V14_000799"
                     /pseudo
     CDS             15688..17040
                     /locus_tag="J8V14_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            17056..18276
                     /locus_tag="J8V14_000800"
     CDS             17056..18276
                     /locus_tag="J8V14_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA0920924.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            18311..18988
                     /locus_tag="J8V14_000801"
     CDS             18311..18988
                     /locus_tag="J8V14_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBA0920925.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            18985..20178
                     /locus_tag="J8V14_000802"
     CDS             18985..20178
                     /locus_tag="J8V14_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBA0920926.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            20193..20519
                     /locus_tag="J8V14_000803"
     CDS             20193..20519
                     /locus_tag="J8V14_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBA0920927.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            20523..20858
                     /locus_tag="J8V14_000804"
     CDS             20523..20858
                     /locus_tag="J8V14_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBA0920928.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            20855..21250
                     /locus_tag="J8V14_000805"
     CDS             20855..21250
                     /locus_tag="J8V14_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920929.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            21247..21618
                     /locus_tag="J8V14_000806"
     CDS             21247..21618
                     /locus_tag="J8V14_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920930.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            21634..22194
                     /locus_tag="J8V14_000807"
     CDS             21634..22194
                     /locus_tag="J8V14_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA0920931.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            22319..22630
                     /locus_tag="J8V14_000808"
     CDS             22319..22630
                     /locus_tag="J8V14_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920932.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            22654..22845
                     /locus_tag="J8V14_000809"
     CDS             22654..22845
                     /locus_tag="J8V14_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920933.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            22845..26693
                     /locus_tag="J8V14_000810"
     CDS             22845..26693
                     /locus_tag="J8V14_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBA0920934.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            26694..27566
                     /locus_tag="J8V14_000811"
     CDS             26694..27566
                     /locus_tag="J8V14_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920935.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            27563..28612
                     /locus_tag="J8V14_000812"
     CDS             27563..28612
                     /locus_tag="J8V14_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920936.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            28616..29440
                     /locus_tag="J8V14_000813"
     CDS             28616..29440
                     /locus_tag="J8V14_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920937.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            29458..31518
                     /locus_tag="J8V14_000814"
     CDS             29458..31518
                     /locus_tag="J8V14_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBA0920938.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            31681..32127
                     /locus_tag="J8V14_000815"
     CDS             31681..32127
                     /locus_tag="J8V14_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920939.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            32129..32266
                     /locus_tag="J8V14_000816"
     CDS             32129..32266
                     /locus_tag="J8V14_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBA0920940.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            32301..32543
                     /locus_tag="J8V14_000817"
     CDS             32301..32543
                     /locus_tag="J8V14_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA0920941.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32559..32756
                     /locus_tag="J8V14_000818"
     CDS             32559..32756
                     /locus_tag="J8V14_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA0920942.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            32767..33783
                     /locus_tag="J8V14_000819"
     CDS             32767..33783
                     /locus_tag="J8V14_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0920943.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            33898..35106
                     /locus_tag="J8V14_000820"
     CDS             33898..35106
                     /locus_tag="J8V14_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920944.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT        12516 a         5900 c         7802 g         9295 t
ORIGIN      
        1 tagaaatgat taaagtaata gcgacttggt aatcgctcaa aatttttatc aagtaattca
       61 ccaccttttt actcaaatta tatcaaaaag gagagaagaa ataatgcaag aattagtaat
      121 tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga tcgtgtcagg gaaataaact
      181 atctttctat attttaccac agaaaggaat gaaccgtaaa tgctaattgc aacggataca
      241 ctagaccgca tctttttaaa agacgaatac aaactgcgca atatagatgc gtcaggaatt
      301 ttagtatttg atctttatga caatggaaaa attggtatct atcaagcaag tgatattgaa
      361 gaaacaaacc ttgctttcga acaaattgat gattctgtgg aattggattt agatgaggca
      421 atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg cacaaaagaa tggcaactct
      481 ttaccaactc agcgagtcat atatcaaagt cctggaactg gcagaacaat tggatgaaga
      541 aattcttcgc gatactctcg attcgattaa tgaagcgatc gagtataagg cagaaaactt
      601 agcaaaaata gttaaagaag tagaagggaa agctgagtta atcgatagtg aaattaaacg
      661 tctgcaggaa cgtaagacat cgcttttgaa caatgctaag agtatcaagc actatttaca
      721 agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt gaattattca acattggaat
      781 tcaaaataat ccagtatcgg tcaatgtaat caatgagaac ttaattccaa aaggattctt
      841 tacccctgtg cctcccaaat tggataaaaa gcaattgaag gaggaactga agcacggaga
      901 tattcctggt gctgaactcg tccaaacaaa aggtttgagg ataagatgat gaaggaggag
      961 aaataatgga acacgaaaac gtaaaagacg ctttgaaagc tgctatcgaa atcgcggaag
     1021 ctaaaggaat aaaggttgac ggcaaaccag caactgtcca tgacattcaa aatttgacga
     1081 aagaacatct ttattttatc gccgacttgc ttggtttgtc ggaattgtat ttagacaaat
     1141 aaccccacac ggaaggagtg gctagggtgg ttgtattaca agtaatagtc tgcgtaacat
     1201 tcatttggtc gtgcataccg gtttgtatat atatcatgaa gaagcttcgc cctgagtctt
     1261 acaaattata cgcaatttac agcataacag tagctcttgc atttacattg tttgcaattt
     1321 ctaggcttcc gttttaatcg tgggaatgaa tttttaatga actaaattat ctataaagca
     1381 aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa caacagcaat catcggacta
     1441 gcactaggta gcggcgttat cggctacgca gcaagcaatg cgtttcaaga tttggacacg
     1501 atcaaagcaa atttcaacac agtcctacaa tacggtcaaa cgaaatcgca acgtgtgtca
     1561 gaacttgaat cacagctatc caacaacact cgcacacagg agcagctgaa agccgaaatt
     1621 gagcaaatca aatcggacaa gcagaaggaa attgaagcca agcaacgaga agtcgaacaa
     1681 aagcaacagg agatcgctac aaagcaacag gaagccgata gcttgcgcca acaactatcc
     1741 acagtgcaaa acgacaagga gcagttagaa cagcgcgtga gcgagttacg gcagtatacg
     1801 gatcaaaagg taggggagtt ggggaaatga aagagttgac tatttatgac ccaaatattt
     1861 catgggctaa acacacaatt aaagtttctt ttatgatttg gggttacaaa ggatatgtta
     1921 cttacaaagt gggcggtaac actaagggtt tatcattaat agctattgat tcagatgatc
     1981 tatatgatgc taatttcgag gataacccag ttaatttccg tgaccttgat gaagattggt
     2041 tctctatgga acttactaac gataaaggcg acagtacact tgtagaagat gaattcgaca
     2101 gactaggtga ttatattgtc ggtgttgaaa ttattgcgca cgaaccagag taggagagtg
     2161 aatacaaaat gattaaaaga atcactatat cagttgaaga agatagtgga tcaacaatgc
     2221 agcataccga caatcgaaaa acttttttaa ccaatagaaa ggagaatttt gatgcagatt
     2281 aagaaggcga cggatatcga acaaaccaag ggaacatatt tgatctacgg tgctccagga
     2341 aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa cactgatcct tgatattgat
     2401 cggacttcaa aagtccttag aggtgagaaa aatgtcgata tcgtatatat cgacaacgaa
     2461 gatacctgga acgactgggg caacacgttg gctgatttaa ctaccaatta tgtaggtgtt
     2521 tatgacaacg tggttgtcga taatgtcagc gaattggaac ggtgtattct gtccagttta
     2581 ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg attatcaata tatgcaattt
     2641 agaatggtga acagcctacg ctatatgaaa aatttagaca gcaaccttat ctggaccgca
     2701 tgggaagaaa tcgatttgtg gacggattcg gatggatctt cttacaacat cgctttgccg
     2761 cagatcaatc gaaagattcg aaacaatatt ttgggattat gtgacgttgt tggtcgattg
     2821 atggtaaagg aagacgatga acgcggattt attttacaag ccacagattc aacctatgcc
     2881 aaaaatcaat tggataatag atctggctgt aagcaaagtg agctgatttt agatggaatt
     2941 gtacgactac cagaatgatt tagtcgatcg tgcaagacaa gcctatgttg atggttacaa
     3001 ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca gtcatggttg cagagatcgc
     3061 taaacgaacc acacgaaaag gcaacagagt gttgttcttg gtccatcgtc gtgaattgat
     3121 tgatcagatc aaagatacgt ttcaaaagat gggcgtagat aatcagcttg taaatttcgg
     3181 catggttcaa acagtcgtac gacatcttaa aaccatcaag aaaccacaac tgatcatcac
     3241 tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa atctatgagt attattcgga
     3301 cgttccgcga ttaggtttta ccgcgacacc catccggtta aacggtagtg ggttggggga
     3361 tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg ttgattgaga atcatagatt
     3421 agctccttat gaatattatg cacctaaatt gatcgataca gccgaattaa agaaagcttc
     3481 gactggagat tttacaaaga aatcaatgga taaggcagtg aagaatacta tttatggcga
     3541 cgtgttgaaa cattataaaa cgttagcaga aggtgaacag gcaatagcat actgtcacag
     3601 tatcgatgca agtaaacaca ctgctgaaat attcaacgat gctggttata aagcaacaca
     3661 tatcgatgca aaaactccaa aagatgaacg tgcagatatt atcgaagcgt tcagaaatca
     3721 tgaaattaaa atcctatgca atgttgattt gatcggagaa gggtttgatg ttcctgattg
     3781 ttcgactgta attatgttga gacctactca atcgttatca ttgtacattc agcaatcgat
     3841 gagggggatg cgttaccgac cagataaaac gtcaatcatc attgaccatg tgggtaatgt
     3901 gaaccgtttt ggattgcctg atatggatcg tgaatggagt ttagatacaa aaaagaaaac
     3961 aaaaactgat agtgatttat ctatcgttca atgcgcgttt tgttttggtg cttacgaacg
     4021 gccaaaaggc gataatattt gtccgtattg cggagagttg cagccgattg aagaacgaaa
     4081 gtcagagtta gaaatcgatg aatcggctga attaatgaaa gtcggagaaa cgaaaattac
     4141 tttgaatttt gagaacaata aatattacaa catgacggaa gatgaagcag aaagtataga
     4201 ggacttatat gccatagcaa gagctaaagg atttaaacct ggttgggctt atatggctgc
     4261 taagaggaag gggtggttgt aggaatgggc ggtatttttg gtaaaaaagc agaagatgtt
     4321 caaggttcaa taataaatgg cgtaacaata ataggtgata ctggaaaaag agatagttca
     4381 ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag tttttgaatc ttctctatct
     4441 aatataaaaa gaggtgttgt taacgggaca ggcgaagaga gtggctacgt taatttaaaa
     4501 aaagctaatg aaaaatacca tgttgaagga acttacgtta aggctttgtc tgtcggtgga
     4561 acaaaaggcg ttaattggga taaacgaaga aataaatgga aagcacgatt atatttcaaa
     4621 ggagaatatg tcttacaaaa ggatttcaac accaaagaag aagcaatcca agcacgactt
     4681 gcagcagaag aaaaatattt caaacctatc ttagaaaaat atgaaaataa aaaggagaat
     4741 gattaacatg acaggattta acttagactt ttcaaaagcg caacaaggga acgaaatcaa
     4801 agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa aatgcaacta aatcaggcgc
     4861 agaatttatc gatatcgatt tgatcgttcg taacgatgtg gatcaaccgt tccaaaataa
     4921 acacattttc gcaaaaatct ggaaagccaa agctaccggc aaatataacg aaggtatgat
     4981 catggcaatt gcacaagcgc tacagttgga agacggaaaa tcatataacg ggtttgatga
     5041 attgctggcg gactttgttt tgaaaaccgc ctcagtacgc gtgaaaactg aagagtcaaa
     5101 tggatataaa aacgtcaatg tgaagtcttg ggataaaacg aacacacggg gcgtaatgaa
     5161 tcaccaattc aaaaatggtg atgaaccatc cttcggaccg gaacgttcaa gagcgacaac
     5221 ggttagaaat gacaatcttc cattttaatt gaggtgtaat caatgtatga aaaaattcca
     5281 aaagagctta aaaatctcaa acaatggtgt gtttataagc tagtttggga tgaaaagcgg
     5341 cagaagcaca cgaagatccc ttataacgcc aacaacgggc ataaagccaa atccaatgac
     5401 gagagtacct ggtctgattt tcagaccgct ctcgctgcta ttgatcacta cggaatgagt
     5461 ggactggggt tctttttcaa accaccttat ttcggcattg atatcgataa tgctgaaggt
     5521 gaagttgagc gatttaaatc aggtgatata gaagaaaata ttatctatga atttatcgaa
     5581 agcatgagat cctatgctga gtattctcaa tcaggtactg gcatccacat tattgctcgc
     5641 ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag aaatgtacca gaacggccga
     5701 ttcttcgtta tgactggaaa tgccgcatca aaatatttag aaataactga gccgaatcca
     5761 aaggatatca aacgcctata tgatcgctac gttggagatg aaaaaatcat ccaattcaaa
     5821 gaagaaaatc cgctgatgaa taccgttgat ttaccggttg aagaaattat caaacgtgct
     5881 gaatcgtctt cccaaggggc aagattcaaa gtctttatga acggcggttg ggaagctttt
     5941 tattcctcgc aatccgaagc ggatatggcg ttcgccaacg acttggcttt ttggaccggt
     6001 agagactttg aaaaaatgga tgagattttc agagcgtctg caatgatccg tagcaaatat
     6061 gaccaaaaaa ggggatcggc aacttacgga gaatctctac taaataaggc aattgctgat
     6121 acaaattccg tctacaaccc caaacggaaa tctgatttta agatttttat caaagaccag
     6181 gaacagccaa aagaggaaaa atactattcg tacgacgaca ccggcaacgc ggatcggttt
     6241 actgatatct acggaacgtt agtcaaatat tcgtacattg ataaatcttg gtattactac
     6301 gatgggaaag tttggctaca ggacaatacc ggcgaagttc ggaaaatgat cgatacgaca
     6361 gtagacatca tgggaaaaga accgttaacg atccctgaag gcgcagacga tgaaaccaaa
     6421 gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt ctcgcagcaa tgccggaaaa
     6481 aacgctatga tggacgaatt gaaacatcga ctatccgtat tgccggaaga gttcgacaaa
     6541 gataaaacgc tgttcaacac acaaagcggc tatctgtctt tgcatgacgg tcttctacac
     6601 gagcatgaga tagacaaaat gtttacacgt gtttcaaatg tcgagtacac agaatcggtc
     6661 gattgtccta tgtgggaaga atttattaat cagatttttg acaatgatca agaattaatc
     6721 agatatatcc agaaatgtgt cggatattcc ctgaccggat ctaccagaga gcaatgtatg
     6781 ttcatcctct acggtcatgg gtcaaatgga aagtcggtat ttttggaaat tatttctgaa
     6841 ttgatgggaa actatgcgat gactatgcag gcacaaacga tcatggtcaa gcaaagccaa
     6901 tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc ggttggtcac ttcgtcagaa
     6961 ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac aattgactgg tggtgataaa
     7021 gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg aacctgaatt caagttgtgg
     7081 ctggcaacga accacaagcc catcatccgt gggaccgatg acggcatctg gcgacggtta
     7141 aatttaattc cattcactgt ccaaatcccg gatcacaaaa aggataaaaa tctgaaattt
     7201 aaattgcaga cggagttgca agggatcctt aaatgggcaa tcgatggttg cctgttgtgg
     7261 cagagagagg gcttggagaa accaaagtcg gttgttgcag cgagtcagga ttatcgaaac
     7321 gaaatggatc aaataggtac atttatcgaa acgtgttgcg aaacaggtcc aggactaaaa
     7381 atatcaggcg gagaattata caaagtttat cgtgaatggg catcagataa tggcgagcat
     7441 actttcacta acacgaagtt tggcagagag atttcaaaga aatatagcaa agaaaaagct
     7501 ggtggattta tggtttataa aggaatcaca ttaaaaccaa gaaaatacga taacgttaga
     7561 gaacttttta aataaatctg gagggttctg gagggttgag aacgaaaccc tccaaccctt
     7621 agaaacgttg atatatcagt atttatatta tatttttctt tctttctgga gggttttaaa
     7681 aaaataataa agtattaaag taaaaaataa aaagtaaatt atataaaaaa gaataggttt
     7741 ttgggtctga ccctccaaac tctccaccaa aatcttccta aatgctttga taccaacgat
     7801 taaacagtct ggagggttac attgaaatca gaacatcaaa tacaagacga gatcagaatt
     7861 gccttatcaa aacatggttg cacgattttt cgatccaatg cgggacaagt gacaacgatc
     7921 gaagggcggc aattttacgg gatgccaaaa gggttcccag atctatgtgg acatcgtaat
     7981 tctgatggga aagcaattta tatagaagta aaaaatgaac gcggtaagtt gcgtgaagat
     8041 caaaaaaggt ttgctgaatt tttaaacggt caacctgtac tatacggtgt tgcacgatca
     8101 gtggaagaag cattgaaaat tgtggaggaa tcatcatgac aacagaagaa gtgattcaaa
     8161 tgcgtattcg aagccttcag cgtgagattg acgatctgga acggacaaag gcagtgatgg
     8221 tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc
     8281 atcgattgga ggaatgagcg tggataagaa agcagcaatg aaacgaatca tcgaactgac
     8341 acattctgag aattggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     8401 atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga aaaattgcaa tctggcatgg
     8461 tgaccgaatt ctagtaacag gtactgctga acagttatct gaaattactg gattaagcaa
     8521 aaacattatc tgggatagag ctaaacgcga gaacgttgat tcgaaaggtc gtcaatttaa
     8581 acattgggag aagaaataat ggaggaacta atcacaaaag tagagcagtg ggcaaaagat
     8641 aagggattgg atcaagctga ttccagcaag caaatgttaa aaacgatcga agagattggg
     8701 gaagttgccg cttctctagc tagaaaagat gaatatggtt taagagacgg aattggagac
     8761 gtagtagtaa ccttgattat tttagctatg caaaatgata tggatttgta cgagtgtctg
     8821 aaccaagcat acaacgaaat caaaggacgc acaggaaaaa tggtagatgg tgtattcgtg
     8881 aagtcgagtg atttggagga cagcgatgaa taaaaaagta ttaattgata aacaagcatt
     8941 gattgatgaa ttaatgaaaa tacctggtgt gggatctaat agtgacgctt tagaaacgat
     9001 taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg cctaagtttg tggcggaact
     9061 actcgactat tatcgtcagt caacagatgt tgatttatta gctttattga taactttcca
     9121 tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa gaagcaattg attggctagt
     9181 tgatcatcct gaaatttata tgcgtgcatg gctgtttggc tacgagctcg agaaagagcc
     9241 gttgtattgc gtaataatca atggtagata tcttgtcaaa gtattcagca atacaaacgt
     9301 tgtcatatta gttccagcag atgaattttc acagtacgtt actcaaaaat ttcaattaac
     9361 tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg tttgctgtgc cagtggatga
     9421 ggtggtagaa ggatgagcga cccatacatt actaaattag aaaagttgtc cgaagatttt
     9481 gagaagaata aaaataaact aactaaagat aaacttatag aactggttag gttggttatc
     9541 agaggagcta ttacaagaga aaaggcgcag caaaacatag aaatttttgc tcgtagtgtc
     9601 cgagatggat tagagaagaa agttaaacaa aatgaacaac agacaccgca gaataacaaa
     9661 actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc
     9721 agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcgagcg gcgctattcg
     9781 taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta
     9841 aaagacggat tttactccag cagtcatggt atcggcggtt taatgctaga tatgccgaca
     9901 aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt tcgctgtgaa
     9961 gcagaaggat tcatctatcc attttgtgga tatgtagaac atctctataa tcactcagca
    10021 atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag caaagagaat
    10081 ttagcagtag cgagattggt ggatatggag gttatataaa cagcgaaaaa atgtacttct
    10141 aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa gaccacagag
    10201 ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata ggtatattta
    10261 acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag cttatttgtt
    10321 ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg aatccaaaat
    10381 aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat caatagataa
    10441 tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa atataaaaaa
    10501 gacagccgac cactgactgc ctatataaga gtattgaaat aaaaagctgc tgatataata
    10561 aattccacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
    10621 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
    10681 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
    10741 ccgaaggcgc cgaagcacgg caataaggca gaagacgcaa tcattcagat gatggatata
    10801 gaagcggaga gagacgcgat tctagcagcc ttgatggctc ttagtctgat tagtcgtcag
    10861 atactctact atagcttttg tgtgccagat agcttctcaa actacagaat tagccgtgaa
    10921 gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca
    10981 gaagcatata aacacggaag aataattgct tataaataat ttggcggctt tttggcggaa
    11041 tgatggcggt ttttagctat ttaccagtga tattatggta gtgtcgaaag attagtgata
    11101 ggtctaagac aaaataataa taaaaggaac atcgttttat tattgtttca caattaagct
    11161 tcgatagaca gcaacggaaa tattaagaat aaggatgtga attttaactc cttctaaatt
    11221 gttcttatta tctatcatcc gttgctgtct attgtttttt taattattca cacgataact
    11281 aaaggtgagc gaagagaaat gattccatta ataatttcaa tttttgcgct ttgtcttaat
    11341 gtctatatga ttggatttaa aaatgggcaa aataaaagat agtagtaact aagaaagaaa
    11401 tttttatatt gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc
    11461 ttcggcaact atgcaatgtt cgattcattg ccagtgactt tggcaaccga ggcatcggcg
    11521 gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat
    11581 tacatattag atcactcgtt gagtggtctt tttattttac ataaaggagg taacaacgat
    11641 gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa acggtaacgg
    11701 tgagatagta cgaaagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa
    11761 caatgaagtt atttcttttc gcagaaggag aaaagccaaa tgagaaacta ctggtatgta
    11821 tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct
    11881 gtacaaatca aagggaagta ctctattgtc gaaatgctaa gagaagctac accaaacgaa
    11941 gtggataaat gcaagctgat atattgcggt catggctatt ggaaagacga gtatatccaa
    12001 caaaacattg agaggtggat agatagatga gttacctcga acatttgaaa cgttgctaca
    12061 tgcattctaa gaataaactt cctgacagct acactgtaga tgatgttgct atacatgtat
    12121 tgaagactga gagccatagc agtcctgatg gtagcagtaa ggagcagacg ctcgcgtggt
    12181 tcaagttctt taagtggatt aaggaggaag agtaatgcac atgcacatag gagaaaagat
    12241 atataaattg agacggctta ataacgaaac aatgaacgac ttagttaaac aaactggagt
    12301 gtcaaaatca tggttatctg atattgagtc tggaaaaaag caacgcgttg attttaataa
    12361 gatatgtgca atagccaaac attataaagt tagcttagaa tactttcggg atgattattt
    12421 ggatagtgat gaacacgagg atattattcc tttgacaggc gaaacaagag ttaaatcagt
    12481 gaaatcgctt gaacaaagcc taaagtatct gaataacaaa caagaaccta accttctaga
    12541 gatcaaacta aaagatactg actcagtacc agaggtttgg tacaagggtg agaggttgga
    12601 tgaattgcct aaaggattag tagatgtctc gtatcattgg aagactgatg attttactaa
    12661 tgatgataga ggagcgaacg acatcacgat tcaatacttt tctagcttta atgataagta
    12721 tccagataag aaaacaatcg gacacaagag agatatgtaa atgaaagaag ttagacctag
    12781 agacgaaata gacaaactat acaagaccaa acgatggcga gacctaaggc aagtagtaat
    12841 agctagggac ttcggcatgt gccaagagtg caagcgtcga gggcggaaca caaggggcac
    12901 gatcatccat cacatagtcg aggcgaggga agacctgtca ctgttctggt ccgtagataa
    12961 ccttgaatgt atctgtgtag cttgtcacaa cagagagcat ccagagagat caggcgggaa
    13021 gaagaaacca aaacctaaat cacatatcgt taaaatgtat tcaactcctg aaagataagt
    13081 ttgcagcgaa atgaaggtag cccccctact ctaaaagatt aaagagtaag gcttgagaag
    13141 aacggtgctg tccttccttc gtaaaaagac cgcttttcaa gttttttgga gaaaaaggaa
    13201 aaagccgacc aatttaagcc ggctttggac gaagctattt cttagtccat ttgtttcctt
    13261 tttgagaagt agggggtaat cggtcgcctg gatcaatttt tacttcttgt ccgccttgga
    13321 catttccacc acgaggaccc acttctttgt aggttccttt tggtttgttg tcttcgccgg
    13381 gtttatagag ttctcccata ggaatccctc cttaacaaat ttcggcacag cactgccgat
    13441 aacctaatta taaggattgt gataacgatt tcaatctatc ttttgaaaga aggtgatatt
    13501 atgccgcaac cagcgaagag tgcaaaatta caattattaa acggaaaccc aaataagaag
    13561 aataccgaag aactccgcaa gcgagcggcc gcagaagaca aattaaaaat ggctactgac
    13621 aaaatcaaac cgccgttatg gctagattcg ctaggaaagg atacctttga gtttatcgcc
    13681 gatgaattgc tgtctgtgga tttaatcagt aatccggacg tccatacaat ggctctatac
    13741 tccaattggt attcgcaata cgtttcttta gaaaaacagc ttcgaaaact acaacgagag
    13801 tacaagttga actatgcgct tgcgaaaaag gaggcagagg cgagaggtga gccgtttaat
    13861 gaacctaatg aattaattgg taatccgctc tctcggcaga tggatacagc gtctcggaat
    13921 ctccgttctt ttggcgctga tttaggacta tcaccagcag ccagagctaa gttagctatt
    13981 aagatggctg atgatggtgg tgatgacgat gacgacttct aatattttgg atatgtctta
    14041 cacagaacgt gtggactatt ggcaaagcta tcttgaggag caagcttctt ggggtggctt
    14101 tttaaaatgt ccatatccgg aattgttaac tacttggtat gcggaacgat taatcgatgg
    14161 aagcatacca gccagcaaag aaaatattca agctgctaaa cggcatatgc gtgatttgca
    14221 gcgccaagga acagatgatt ttccttggat ctttgacgaa gaaaaaggtc accggcctat
    14281 tagatatatc gaaaaaaaat gtaaaccaac tgaaggcgac tttggttcgt ttgttttgca
    14341 accttggcag catttcataa ttggatccat gtacggatgg gtacatcgtg atacaggaga
    14401 gcgtcgcttc cgcgaggctc ttatttttgt tggacgtaaa aacggtaagt tttgccgttt
    14461 aaaaatcggg caaaatcggt gaaaaccttt atttttggct ttcttttagt aataattcga
    14521 tagctttatc aagcaattta gacataggaa ccatagtttc ttccgacatt ttttttaatc
    14581 cttcgtataa atctttatca attgcgttag aaattctaat tctgtttttt agtccgtatt
    14641 tgtccatgtg tgacactcct ttttatttta ctataacatc cgattccacc gcttgcaact
    14701 accatcaatt gatggtagta tataaaagag gtgagtagat tgaaaaaata cattatttac
    14761 aaacacttaa ataaaataaa tggcaaaatt tatatcggtg tcacaaatga tattggcaga
    14821 aggtggagga gcggtgggat tgaatataaa ccaccaaaga acgaaaccca acaccacaga
    14881 agtttttgga atgcaataca aaaatacggg tgggaaaatt ttgatcattt gattattgaa
    14941 gaagacttaa cgatgaaaga agcttttgag aaagaaaagt tttatattga actttatgac
    15001 tcaactaata agaaaaaagg ttataacatc gcaaaaggcg gaaatggcgg aataatatat
    15061 aaagttcacc ccaaaggaat gctagggaaa aaacaatcta aagaattttc ttcaaatcac
    15121 agtaaatggg caaagaacca taagaataat tgtatgacaa acggtgatgt tgtatggggt
    15181 gttactcacg aacacccaaa aggcatgttg ggaaagcacc acagcaaaga atcaattatg
    15241 aaaaagaaag catatagtgg tgaaaatgct gtaacctcaa aaccaattgt cgcaattgaa
    15301 cgggatggaa ctaaaagaga atttcacagc gctaagcttt gcatggctta ctattcgatt
    15361 agtacatgtg ttttttaccg acttttaaaa gatggcgccc cttatgtgat aaatcctaaa
    15421 gcgaactaca gaaataaaga aaaaatatta gctatcgaag gaattatgtt caaacataaa
    15481 gaagataccg aggtaagtta gtagattgcg aaaggttact aactaccgta acgcgtagga
    15541 gttgaataaa tataatgctc ccaagagtgt ccgacagcag ataatttgct gaaaatgtac
    15601 gctaaactgg gccagaactg actgaccgat gaaaatgagg gtgacctcca gagtgcgaga
    15661 taaaaagctc gcagataata acaatcgaaa acatcactaa tttcaggcct ttccacatac
    15721 atggtcgctt atgatgatga acaaggcgcc aacgtttacg tattggcaaa tgctcgtgat
    15781 caagcaagct tgttgtttga taaggccgca gaaatggtca aacaatcgcc ggcgctcttt
    15841 aagaaatttg gtaagcctaa acgatcaagt attaattatg ctcccgcctt ttctaaaatg
    15901 gaaccacgcg cctcagatag ccggaaattg gatgggctaa acactcactt tggtattttt
    15961 gacgagatcc acgagtttac gaattacaat ctgatcaacg ttatcaagaa atcaagagga
    16021 accagaaaac agcctctgat agtttatatc acaactgctg gatatgtatt agatggtccg
    16081 ttgatgtctt attttgagca aggtgtggac tgtttggaac atttggaaga tgacatcgat
    16141 gaacggactt tctattatct ggcaaaactt gacagtgcgg aagaggctga tgacccaaga
    16201 ttatggatca aagccaatcc gaatatttgt ctaatgaatt ttgttggcat gctagatgac
    16261 tatgttaagg ataaaaaaga tccaaaagaa tatgctgact ggattaccaa gcaatttaac
    16321 ttgttttccg atatcgatga gctgtcattt gtcgatatgc ctaccattaa acgaaacaat
    16381 aaaaccatcg atattgaaac gctcaaaggt aagaagtgtg tcggtggttt tgacttgtcc
    16441 gaaacggaag actttaccgc agccgtttta gaatttccgc ttgaaacagg cgaggtattc
    16501 attttgcaac acacatggat cccacaagct agatttgatc gagataacaa tcaagagcgt
    16561 atcaaagcgt gggagaaggt gggagatcta acgattattc ctggtgatta cgtcaattat
    16621 gaatacgtct taaattggtt tgtagaaaat tcgaaaatct atgacattgt aaaaatcaat
    16681 tatgacaagg ccaaggcgct acgattaaat aaagaactag aaaatgcagg atttgaaacc
    16741 aacgagattc ggcaagggtt tctatcatta ggtgggccaa tgcaaaactt caaggaaatg
    16801 ctattggacg gtaaggtgat tttcaacaat tccaagcttt accgatggta tctatccaac
    16861 gtcaagctgg tgatggatcg caactcaaac tggatgccgt ctaagcagtc caagagtaga
    16921 aaaatagatg gttttgcagc aagtttgaac agccacgccg aagtgttgaa tatgttggtt
    16981 aatcctgtcg gaaccgggaa agtaacctat tactcgattt ccgatttaat gaatatgtaa
    17041 gaaaggtgtg gaggaatgag tattttagat cgtttgcgtt cttttggccg agcgaagccg
    17101 aaagcgagca aacaagagta ttttttgaat gacccgggat tgataccgta tttagtcgga
    17161 aaagatgaaa tatcagaagg gattttttcc gtaattagcc gtgtatcgaa cgtttttgcg
    17221 tctctccctc tcaaaatgat agatgtggag tttggccaac cggacgactg tcctgcatac
    17281 aacttgttga gcgaaggccc tcgatatttt acaaagtttg attttttccg ggacgtggaa
    17341 gttttgagaa actaccaagg gaatgcgtat gtgcagattt tccgaaatat caatggagaa
    17401 gtagcagata tggcgttagt aaaacctggt gcttgccatc cagtgattga tatggatagc
    17461 ggggagcttt actaccaagt aactgcgact gacaaaggca gttacaagca agttatctat
    17521 gtacattaca tggaaatgct tcactttaaa caaccgaggt ttggcggctt ggaaggtaca
    17581 gaccccacaa aagtattaac gaataccctc ggatatgatc gagaagtccg aaaaatctct
    17641 ttaagtcagc ttaaaggaag taatgaaggg ctaaaagtta agtttgctag caatatggat
    17701 gaagaagcta aaaaagctac agttaaaaac attgctgatt tttatcgaca aaacggtgga
    17761 ctacttgtgg aagaaaacgg tgtagaaatc gaacgtttac aacgagagct ggtagacagc
    17821 aagcttttag atactgataa agtatctcgc tccagaatcg ctatggtcta caatgtgccg
    17881 gagcatttca tcgggaataa ccagtcgagt tactcctctc aggaacagct caatatggaa
    17941 tttttgacgt acaatctagt accgaccgtt aatcaatatg aagcggaact aaataagaaa
    18001 acactatcga gaaccgaaaa agcaaaaggt tatcgatata agtttaatgt cgcaagtttg
    18061 ctaagagctg acactcaggc aaggggacaa ttctatcaaa tcatgcgacg aggcggagca
    18121 tactcagcga atgatgtccg aagatttgag gacttgcaac cgataaataa aaccggtatg
    18181 gatgattacc atatttccgg agacctatat ccaatcgata tggatccaac attaagaaaa
    18241 acaacctcgt ctaaaagcgt agccgaaaac ggttaggttt ttttagtttg caccgaaggg
    18301 aggtggaaaa atgaaaaaag tgacgttaag cggcgatgtc gtggataacg ataccgcgtg
    18361 gctttatgac tggtttggga tcaattgtat ctcaccaggg aaaatttctg ccgctcttac
    18421 agaagcagcg ggggatgaag tagaacttga tatctcatcg aacggtgggg atgtcctagc
    18481 ggcaagcgaa atatataccg ctatccgtgc ctatccggga aaggtatccg gaaatgttgt
    18541 gagtattgca gcaagcgctg caagtgtaat cgcttgcgct tgcgaaccgc ttagaatctc
    18601 acctacggca cacatcatga ttcataacgc atgggtgacc actagtggca acgctgagga
    18661 attaaaagcc aatgcagaaa tgttaagcag tgtggatgag tctattgtta atgcttacga
    18721 gatcaaaaca ggactcgatc ggaaaaaact tgctgattta atggcgaaag atacttggtt
    18781 aaatgctcaa acagcagtag cagaagggtt tgcagatgaa attatgtttg cagaagcacc
    18841 agtaacggta ctcaatgcct ctcaaccggt tattccaaaa aacgcagtaa ctaagttgaa
    18901 aaatttaata ctcaaagcgg aagcaccgca agaagaaaca ctcttacaga aaaaactaaa
    18961 agccttaaat ggagggaaaa acgaatgaat ttagaacaat taaaaaatgc gtgggtcgag
    19021 gcgggaagta aagtctctga cttaaatgca caactcaatg cagcattggt tgacgatgaa
    19081 aaaacagaag aagatgtagt aagtttgcaa gcacaagtaa aagcagcacg ggctaaacgg
    19141 gacggattga aagagcaagt ggcaaatatg gaagccgaac aagtcttaaa cgtcaaaaaa
    19201 gaaccattag ataaaaaaga tgaaaacttg aaaaacaagt ttatcaaaga ctttaaagcg
    19261 atggtcaatg gtgatcctgc tattatggct actttgacat ctaatacgga tgaatctggt
    19321 aatgctatcg gattgactat tcctgtagat gtgcaaacga ctattcatac tttggttcgt
    19381 cggtttgact ctttgcaaga atacgtaaac gttgaaaaag tgaccactac cagcggttct
    19441 cgcgtttatg aaaaatggtc tgatattaca ccgttgactg ctttggatac tgaagacggt
    19501 gaaatcccag caaatgatga tcctgcactt tacttgatca aatacttgat caaacgctat
    19561 gcaggtattt ccacagtaac taacagcttg ctaaaagata ctgccgaaaa cattttggca
    19621 tggttgtcta aatggatcgc gaaaaaagta gttgttactc gcaatacaaa aatcttggca
    19681 gctattgatg gaatcaaagc ggcacaaaag aaagatgtta cagatgttga tggaattaaa
    19741 gatatcgtaa acgtccaact tgatccagct atcgaagcta catctatgtt tattacaaac
    19801 caagatggct tcaatgtttt agataaagtg aaacgtgctg atggatctta cttgttacaa
    19861 aaagacgtag cttctgcaac tggatatacc ttcttaggta aaccaatcaa gaaaattgct
    19921 tctcgtttct tgccaaacaa aggtacgcaa gctactccta aatatccact gtacattggt
    19981 gatctgaaag aagccgttac attgtatgat cgcgaaaaca tgagcttgct gacaacgaat
    20041 attggtggtg gagcttttga aacagacacc actaaagtac gcgtcatcga tcgcttcgat
    20101 gtgcaactag ttgatgatga agcggttgtt ttggctactt ttacaactat tgcgaacgag
    20161 acaccggcga aagtttaagg agctgattcc ttatgattct tgatcctaaa atggatttag
    20221 gcgaaatcaa aaacgcacta aagattgata ccgatgatga cgatgtggaa gtaagccgtg
    20281 cggcacaagc tgcaattgca tacattagag gggctatcgg gaatgataag ccctcttttt
    20341 ataagcaaga aaatgaaacg gttgatctga ttaatttagc tatcctgcaa ttagcagatc
    20401 attattacaa cgctcgatca gcaaccgtga gtgggaactt gcgagagtac gatttaggtt
    20461 ttacaagcct aatcttgcaa ctcaaagcaa gttatttgct ttttgtggag gaggagtagc
    20521 gtatgcccct tatccaaaca ggaaatttaa atcaacgcat caagtttgtc cgagatacga
    20581 cggttaagga tgaggacggg caagttgtcc cgacttctac aaccattctt acttgctggg
    20641 caagtgtgca gacacaacgc ctgaacgata ttaagacgtc gattggtacg gctttggaag
    20701 gaacactgac gttcattatc cgctaccaac aaaaatcaga gctgaccaat gatatgaaag
    20761 tgcgttggaa tggaaaaacg tttgaaatca ttacgattac gaaaggcgag tttgcgaagg
    20821 acttcacgac aatcattgca aaagaggttt caaaatgagt gtagaagtcg atgcaaccga
    20881 agtgtacaaa gcgcttaggg aagtaaaagc aaacgttcaa cgagtggaaa gcccagcact
    20941 tagaaaagct ggggagtacg ctcaagaaga gttacgacaa aacacacctt actgggatgg
    21001 aacgaagtca aacggtaaac gtggttcgta tatgcaagaa catgctaaga accatgtggt
    21061 tacaagctcg ataaaaaacg gattggtaga agtcggctat gacaaagatg tttcttggcg
    21121 gatgcacttt atcgagttcg gaacaatcaa acaacgtcca aaaggtttcg tacaaaaaac
    21181 acaaaagcaa atcgaaaaac aagtaacaca aatcattgct gacgaagtaa aaaggaggct
    21241 aggactttga aaacggcagt atcacaagtc tattcaattc tgaatagcaa tgaaaaaaca
    21301 aagaacattg atttttacac caatagtgtt ccggaatcag ctcaaacagt acctagcctt
    21361 ccagttggca gaattacaga gatatccggc aactatgaag attttgcaag caacaatcct
    21421 ttgaccattc aatttaacgt acaggtagat gtatgggtgt caaccatgaa agaggttgat
    21481 gccttttatt ttgcccttga tgaggttatg agggggaatg gttggcaatg cgcatacacg
    21541 gaacaaacag atgacgagga cttggaaggt gcaaagcgga ttatcaaacg atatgtagca
    21601 aatatttcac taaattaaaa ggagagaaaa tagatggcaa cagtaggatt cgagagcgtc
    21661 atttttggcg taaaaacagg tgcaggcggc actctaaaag aattagtagc agataagtcg
    21721 aaaggcggag cgatcgaagc taaaattact ggattaggcg caacttctaa cacaacatac
    21781 gcttcaaacg taccgttctt cattgcaagt aaaggggttt cgtcgccaaa agttacgctt
    21841 gacgtggcag acttaatgga taacggcatt tacagcgaaa tcattggtgc taaaaccgtg
    21901 gatggtgcaa atgtaattgg ttcagaaact gaagcgcctt acgtgtcggt agtcatggtt
    21961 acagcgaaca aagaaggaaa acgcttgttc atgggattga caaaaggaaa attcagtcat
    22021 ccagatatcg acatgaaaac agctgaagac aaaggggtag aattgcaaac cgattccatc
    22081 gaaggggaat tcatttctga tgaacgtggc tatgtatact taacagccgt agaatcagaa
    22141 ggaatgacct tacaaaaatt caaggacttg gtaaataaca aagaggggga gtagttaacc
    22201 ctgcatctac accaacgaca gatacaggga caccaaaaga accagaacca aaaattgata
    22261 cacaaggtta gccatttttg gctagcctta ttttttgtaa aaacaaggag gaaaacaaat
    22321 gattgaattg caattgaaac ttgacggaaa gaaaaaaaca ttcaaacaac aagatatttc
    22381 cgcacgtgca atgcgtgagt gtatcaaatt ttacgagaaa gcggaaaaag cagacctaac
    22441 tgatttagaa gcgattgatt caatgattgc aattacagca gatattttcc aagatccagc
    22501 agttacattt gatgctattt tagacggttt gactgcgagc gagttagtac cggcattaga
    22561 aagtgttttt gaacaaataa atgaactggg aaacaatgaa aaaaagcaga cggcgagcaa
    22621 aaagaaataa gtttttctga agctaggaaa gcaatggatc aaatctacaa agatttaatc
    22681 gaagcaggtt ggacgatgag agatgtggac gaagccgact atcattattt gttacacctt
    22741 tttggagaag tggagagtgg cgaagaatat gtagatggtg ctgatttcat caaacaattt
    22801 ttatcggctg aagacttagt aaaacttgag gaaggaggta aataatggca ggaaaaggac
    22861 aaccggcagg aaatatcaag ctagggatta gtttagatag cactagtttt ggtaacacgc
    22921 tggacgaaat caatgcgaaa gtcaaacaag ctgagtcgaa tatgcgtgcc aatctaaagg
    22981 cttatgattc agcaggacgt tcatacgaag cacttagtca aaagacgaaa gacttgtcta
    23041 cggttatgga agggcaaaac gccaaagtaa gagaattaac aaagcgccgt gatgaagcga
    23101 ttagcaagta tggcgaggaa tcgaaacaag ttgctaacct taacacacag ataaacaatg
    23161 ctaccgcaaa atataatgct tacagtcgcc agttgaacga cacaaaaaaa gaattggtgt
    23221 attccaaaac agccgtcaat gatttatcta atgaaatcaa agaaaatgaa cgacaaatga
    23281 acgccgaagt aaaagcgttg aaagccgctg gtgatgaatc tggtgctttt gaagcaaaac
    23341 aaaaagggct agccaaacaa acggaattat ccgagaaagc tatcgaagaa cagcgcaaag
    23401 ttgtgaaact gatggctgat gagtttggcg attcagcaaa tgaaaccgaa gatgcaaaaa
    23461 gggcattaga aaagttagaa cgacaaagcc aaatatctag caggcaatta gaagcactca
    23521 aaagctccag cgatcaatca ggaaaagaaa tagaagattt tggcgacaag tccacaaggt
    23581 cagctaggaa actggacgga ctaaaagaca aattaagctc gctaaaaagc gcattttcgt
    23641 ttggtgcagt tgctggatta gcgcataacg ctattagcag tgtagtaagt ggcgtgcaag
    23701 gtttggttgg cgaagcagta aacgcatcgg attcattgat gaagttttcc aaaaccatgg
    23761 agtttgctaa ctttgggaag tcacagatag aaagctcgaa aaaagaaatg aaagactacg
    23821 ccgataagac ggtttatggt ttagaagaaa ttctgaacac aaccgcacaa ttggcatcta
    23881 atgggattcc taactataca gaactaacca aggcggcagg taacttgaat gccgttgcag
    23941 gcggttctag tgatacattc aaatccgttg ccatgatgct aacgcagacg gcaggagctg
    24001 ggaaactaac aactgaaaac tggaatcaat tagcagatgc gataccgggt gcttcaggat
    24061 tgttacaaga cgctatgttg aaaaacggag cttatacagg aaacttccgt gatgcaatgg
    24121 cgcaaggtca aatcacttcc gacgagttca accaagcaat tgtacagtta ggtatgaatg
    24181 acggagcagt taaggcagcc acttctacag acacattgag cggttcttgg gagcagatga
    24241 aatccactgt aataaatggg ctacaaagta ttatagaaaa aataggcgtt gaaaatatca
    24301 ctggtttcat caacagagta acaaaaggga ttgaaaattc tattcctaaa attactcaat
    24361 ttataggttg gttgagagat attggaacgt ggatcgttga aaatagagag ccactaacat
    24421 ggattgtcgg aatcataggc ggaattacat tagcagtaaa agcattgaac gtagcaagta
    24481 tgttgctggc aattactggc ggaacattgg cagccccttt tgtggcgatt ggtgtagcat
    24541 taggcgcact agcaggtgct ttggtgatag cttatacaaa atctgaaaca tttagaaata
    24601 tagtcaatgc ggcttttaca gctgtgaaaa acgtagttat gagcgttatc aataatttgg
    24661 tggaatacta caaaatgttg tggagcgtgt tgcagtggct ttgggaaaaa ataaaagaat
    24721 gggcttcatg gattggcaat aaattcattg aaatgaagaa cagcgttgtg aatacggttc
    24781 agaacctatg gaatggcgtg aaaaacttct tcagcaatgg cgttggagat acgtggaata
    24841 aggtagtcgg ttgggtaaaa aacattttca acaaagcaac tgaattgaag aacaacgttt
    24901 ctgatgtaat cggtaacctg tggaatggta tcaaagacac attccgtaga ggtatcgata
    24961 cggtattcaa ttggttttca gaactaccaa cgaagatgaa gaatgccatt attggcggta
    25021 aaaacgccat tgttgatgcg ttcaaaagta ttttcaacgc agcacttaaa gcgataggta
    25081 aaccagttaa cgcaatcatc catggagctt catgggtact agaaaaattg ggcgcagaac
    25141 ctctacaaga atgggatgta ccacaatacg ctacaggtac accagcaggt gggcacccaa
    25201 tcaatggtcc aatgatggtt aatgatggac gtggagcaga aacagttatc acaccagatg
    25261 gtagagcctt cattcctaaa ggacgcaatg ttgttctaaa cgcaccaaaa ggaacgcatg
    25321 tcttgacagc agaagagacc gcgcaacttc aaggttctaa agctcctaag tatcgttaca
    25381 aaaaaggtac taacttcttt ggtaacatgt gggatagtgt gaaaaaggtt gctggtaatg
    25441 taggtcacac aattaaaaac gtagtaggtg acgtgtggga ctttatttca gacccgggag
    25501 cgttagctag aaaagtactt ggggggttag atgtattagg tggattgaca aagtatccat
    25561 tagaagtagg taaaggcatc ctatctaaag caacaagtgc actgactgaa aaaatcactg
    25621 ggttgttctc atctggtaac ttagatacct ccgtaggaac aaatggcgtc tataaatatt
    25681 tggctgatgt tgctaagtct gtgatgaaga aattcccagg atttgtggca actagtgggt
    25741 atagaccagg tgacccttat tcacatggta aacgtaatgc cattgatatt gcattaccag
    25801 gcgtcacagg aggctcacct agatacacag aagcagcaaa ctacgctttt gacaaattcg
    25861 cttccaaaat tggttacgta atcactaatg ggaaagttcg tgaccgttca ggacaatcag
    25921 gtcaaccagc aactggtgca tgggagccat ggcccgatgg agatcactat gatcatgtgc
    25981 atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc aggagatagc gtgggaggca
    26041 gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt gaaaatgacc ggtcaataca
    26101 gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac agagtcaaat ggtaatccta
    26161 atgcagttaa caattgggat attaacgcca aaaatggaac accatcaaaa gggttgctcc
    26221 aagtgattga cccaacattc agacagtatg caatgccagg attcaacagc aatatttttg
    26281 acccactatc taacatctta gcttcaatca gatacgcact atcaagatat ggctcactaa
    26341 caaatgccta tcgtggagtt ggttacgcaa acggtggaat tgtaaaccaa catcaaattg
    26401 cggaaatcgc agaaggaaac aagccagaaa ttattattcc gttagataag gctaaacgat
    26461 caagagcgat gcaattattg tctatcgcaa tggacaaact aggagtttct cctaaaactt
    26521 atggaaatgc gacaactgtt tccagtgatt acgaaatcct agaagccatt gaaaaacagg
    26581 cggcaacaac gaatcagctg ttatcgttat tgctggcatt tttcaaagga aatagccgaa
    26641 ctgatagaga tttagcttta gatattcaga agatcttggt taggaggatg tagatgcgaa
    26701 ctgtattact gagaaataga ataaacgaag aaattgattt gtccacagaa gactattttg
    26761 ctactgggtt gagtaatatg gggtttgaag taaaaaaaga acatgtggga cagtggggga
    26821 atttcagaga aagcagtgag agcgttgaaa tatctgaatt tcagtcatct gtaatcattt
    26881 ctgtgcatgg gtttcgagaa aaagaactgt acaattcact tgtgcagttc ctatcggaag
    26941 gtccgtttga actggaattt gcttttgacg gcgaaacaat ggtaagaaga tgcagtctaa
    27001 aatctttaag taagactgaa atcgatccga aaacatcgtt actaacagac actttagaac
    27061 tatatttcac atcaaactgg tattccgtaa aacgcgaaaa attaattcag cgaccgaatg
    27121 tagtgaaaac acgtggtaag gtttttccgt ataaaagatc ttatatctac actcaaaact
    27181 tgtgggagaa aaaaggtgta ttcaaattca ataataattc tgtataccta acgaatagca
    27241 aggaacggat gtccccgcta aaaattcgtg tgattgggaa gtgttcaaat ccgtattggg
    27301 aagtaatcca aaactcacaa atcatcgcaa cggatggata cttcatagat atgactgaaa
    27361 cacagactct agaagtatca agcctttttg aagatacaac ggcgatttta aaagatattg
    27421 caggggtaga atcttctgtc taccaacaac aggattatac aaaaactaat tttgttcagg
    27481 ccccaactgg agaattcagc attgtgtttc atgttggggg ggcagacgta gagattgagt
    27541 tatacgagga gcgtgatctg ttttgatttt agcagtcacg ctttttcatc gtgatttaat
    27601 gctttataac gaacatttat tctctaacag ttttgaattt ggtgtggacg agatcaacga
    27661 agaggctagt agctttacga tggataagta cgttcctgta aaaacaggcg attttctact
    27721 agcaaaatat attcctagtg gaaaatttgc atattttggt gtaattacgt cgcaggaaga
    27781 cgaaaaaatc agttgtaaaa gcttacttag tttagctgat agtgagatac cgactgcacg
    27841 tgtgtcagga gataactacg aagagcatat ccggcggttg attgaatact atttgctgaa
    27901 tgatccaacg aaacaactaa aagatatttt agacgtcaaa gcagaaagtg cgacctctca
    27961 ttcgtatcaa gctaccgata cgaataaaca caagttaagt gcgtatattc tcaatggttt
    28021 caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa aacagaaaaa tttatacagg
    28081 catacgtgct gtaaatgaat caatttacat taaagacaat tcttctgaat ttagcgattg
    28141 ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac aagctattaa tcgttgataa
    28201 agcaatgaaa gatatagaga aaccgataat actgtcaaca tggtatttgg acgaagaaaa
    28261 caatttgaca caagatggat cgaaagaaaa tatcacgaaa ccaactgtca atttggttaa
    28321 tatctacgat caaaccgcag aagataaagc atcctacgaa gatgtggcaa aatcagagtt
    28381 gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt gtaagaaatg caaaaaattt
    28441 gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt tcttataaag gaaagatata
    28501 caagtcggtt ttgacagctt ggcgaatatc aagtgataag gagtttgtgg aattgacttt
    28561 cggaaacata agaagtcgtt ttatggatta ttttgaagat aatgggggat aaaaaatggt
    28621 tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta agcgcagaaa atgatgctag
    28681 actttatcac gttttatata atcggaaaaa tcaggttatt gatggttacg atcagtctat
    28741 gaatttatct tcaagcggat taacagtaaa agttgctgca ggagcagcga ttattcaagg
    28801 tcgtatggtc gttgttcgac aagaagaaag tataacagtt ccagcaaact caagtggtta
    28861 tatagcatta acagtggatt tgacacaaga agttatacct ggatctattc ttccagaatc
    28921 ggaagaatat gaatggacta ataatcaagt caagctagaa tttataacaa aagttataaa
    28981 aggtaattta aacaatggtg ataaggtcta taatttacca ctatgctcag ttaattctac
    29041 tggatcaact gtttcaatct caaagatatc ggatagttac gagctgactc tctctaaagg
    29101 agaaattttg tggaggggga ctgcgttaat gcatgatact caaactgtcc aaccttcaaa
    29161 aaaaatttgg cagacagttt caggtttttt gttagtatgg ctcccatacg aaaacgggca
    29221 agcaattgag gatagatatg taactacgcc tttttataag gagcgtgtaa cttttactaa
    29281 tgttttagga gaaattgttt caggatttga tgactatcac aaaaagtggt ttagtaaacg
    29341 aataaactat aattcgaata ctaatatatt tacaggtgca gcaagcaatg caagtggaga
    29401 taacgcaaat atggtgctta ggtatatagt ttctttttag ttaggaggta tggaagagtg
    29461 gcaaatttag aaattaaatt atctgcaaat aagagacagc cttatctacg tcaccgtgtt
    29521 gttggtagag ttggtgatgg ggggctcaca acaatcaatg tacaacttct tgaggaagat
    29581 gaaattacac cttttgtaat taatctaaac ggtactttga aatttgtggg cgaagtttca
    29641 aacggtaact ataccgaggg agaaccagaa ataatcgatt cgactaatgg gttaattagt
    29701 tacacgttca ctaagtcaaa tttcagcacg agtcatcaat tcaaacaagc atattttgaa
    29761 tatgtagatc ctaacggcaa aaaagtaact tttcagaact tcatcataga cgtgttagaa
    29821 cgagtggata ttaattcgga gcaagcgaaa tactatattt cttcattgga aaaattacag
    29881 agtgaaatgc aaaccacttt caatcagttc attagtgata aacaggtcca atacgatcaa
    29941 atctactcga aatataacga attagtaaga ttgataaatg aatcagataa gcaagtaaat
    30001 gatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg gcaagctgaa aaagatgtac
    30061 agtaacagca tcgactttgg gggctatgat tattcgggga atccgaattt gttaagtaag
    30121 ctatcatacg acttaattga aaatcaaaat acttcagctg gaacactttc taagggtgaa
    30181 aactcgttta aatataataa gatatcagct gaagtggaag gtggagtaga gttatattat
    30241 aaacgaagag gtatagctaa ctggttaccc tctaataaaa cgcttgtaat gactgttaag
    30301 cttagagctg gagcggacta tagtccagtt gacggaaaac ttatactgat tagatatagg
    30361 tatgttgaca gtggaactgg caagattgtt ttagacttac ctattaacag taattcgata
    30421 actcaggaat ggaaagagtt tagtattact ggaactactc caacatttag cccacaagca
    30481 taccatcctt ggatacaatt tagggctcaa gatggggtac ttggggaaat agaaatgagc
    30541 tatgacatca aaatcgaaga aggctctaca gctacaccat atcagcctaa cttacttgat
    30601 gactcttact ggctaggtaa aacaccgttg ggtgagaatt tgataacgaa tgccaagttc
    30661 ccaattataa cacgaagcaa tcctatttct ggttttgata tttcagaaga attaataata
    30721 ggtgaaactt atacggtatc tttaaaaggg actaagccag ctactaaaga attccactta
    30781 tactacggag aagcaaatta ccagcagttg cagtatcagg caactttact tcctgtggaa
    30841 ggattaacag atgtgtggag tgccactttt acagccagaa atactagtga aacgacaaat
    30901 ttacttagag tagcgttatg gcaaaaacct aattctgcaa catatgatac tgttcaaatt
    30961 gactggctca agattgaaaa aggcgacaca cgcacaccaa atatcagtca gtttaaatac
    31021 tttggtgaag gcttgaaaga cagtaacaat ccgaacgact acagctggga tgtcacacca
    31081 gagtatactg aaaaaagctt gaataatacg gttagtctga ctgaaccgca atccatcgaa
    31141 ggattgaaaa atttcgaaga tggtattcaa tcaaaaggaa aatctgtatt gacatcagac
    31201 gacaacaaat atgaagtcgt aaccttaaca gttacaaacg ggaataccgg atcagcaaag
    31261 ctttatcgtg aaggaaaaac cgtcactatt tatttttttg cgttgaatgg gaaaagcagt
    31321 ggtggaaatg attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt
    31381 gagcaactgg ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt
    31441 gcagatggag ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca
    31501 attacttaca cgatttagaa aggcgtgaat cgatatgaag gcagcatata gaccaattga
    31561 accttacgga ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac
    31621 agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa
    31681 atgaaaaaca tttggaaata tggccgtact ggcggagagt atgcaggaaa agtattggac
    31741 gacatgcttg tatccgttcc ttacacagat cagccaccgc tcgaaggggt tcgttcagat
    31801 ggcgaaccgc taacgattgc tgatcagatg tttgatccta aattgaacca atggattatt
    31861 ttagcgaacg cgttagatca caacgattta aacaatctca aagcgatgta cgaggctctt
    31921 gaacatgaaa acggcaacct aaaacagcta aatgccaaac tcatgctaag cgatgtagcg
    31981 attaaacagg aaaatactgc attgaaagaa aaagctgaca gtttagcaca aatcaattca
    32041 aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta
    32101 aatccagctt caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta
    32161 tgattggggc tgttttactg acgatcaagt tcgtctattc gttccactat gtatcacaga
    32221 tgaagaagca gatagaatta ttagcaaaga agagagcgca tcttaagtga tgcgtttttt
    32281 tgttggaaag ttggtggaac atgaaagaag aagcgctcca agacgttgtg gagagattag
    32341 taagaattga aacaaaatta gacaactacg aatcacttag agaaaaagct gatagtgcaa
    32401 aagatttggc agacaaagcc tactcagtag cgctaaacaa cgcagaagat atcaaagaaa
    32461 tgaaagccaa taataaatgg tcgtggggtt acatgatcgg tttaggcatt acgatcattg
    32521 gctatttctt gactaaattg taaaggagtt aagaagaaat gattttaccc gataagtatt
    32581 atcaagtcat taaatggacg gttttaacag tattaccagc tgcatctgtt ttagtagcca
    32641 cgttaggaaa agcgtatgga tggaatggaa cagatatgac agtactcact atcaatgcag
    32701 tagcaacatt tttaggcgtt atcactggtg tgtctgcata taacttaaaa aaataggagg
    32761 aaaaaaatga agaagaaaat cattttatca ttgagcctaa taatggctct ttttttattg
    32821 ccaattaacg ggttcgccta tacgatcaac aatgaattta atttaggtgt aaatgaaggt
    32881 agctcacaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
    32941 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
    33001 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
    33061 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
    33121 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
    33181 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
    33241 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
    33301 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
    33361 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
    33421 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
    33481 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
    33541 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
    33601 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
    33661 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
    33721 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
    33781 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
    33841 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
    33901 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
    33961 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
    34021 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
    34081 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
    34141 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
    34201 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
    34261 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
    34321 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
    34381 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
    34441 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
    34501 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
    34561 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
    34621 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
    34681 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
    34741 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
    34801 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
    34861 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
    34921 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
    34981 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
    35041 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
    35101 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
    35161 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
    35221 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
    35281 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
    35341 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
    35401 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
    35461 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttc
//
LOCUS       DADQRX010000017        34932 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.017, whole genome shotgun sequence.
ACCESSION   DADQRX010000017 DADQRX010000000
VERSION     DADQRX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.017"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /locus_tag="J8V14_000821"
     CDS             156..1862
                     /locus_tag="J8V14_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HBA0920945.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="J8V14_000822"
     CDS             complement(2020..2922)
                     /locus_tag="J8V14_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0920946.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="J8V14_000823"
     CDS             complement(2919..3623)
                     /locus_tag="J8V14_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBA0920947.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="J8V14_000824"
     CDS             complement(3654..4295)
                     /locus_tag="J8V14_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBA0920948.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="J8V14_000825"
     CDS             complement(4333..4647)
                     /locus_tag="J8V14_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920949.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="J8V14_000826"
     CDS             complement(4635..5264)
                     /locus_tag="J8V14_000826"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBA0920950.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="J8V14_000827"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="J8V14_000827"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBA0920951.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="J8V14_000828"
     CDS             complement(5984..6916)
                     /locus_tag="J8V14_000828"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBA0920952.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="J8V14_000829"
     CDS             complement(6913..7689)
                     /locus_tag="J8V14_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBA0920953.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="J8V14_000830"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="J8V14_000830"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBA0920954.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="J8V14_000831"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="J8V14_000831"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBA0920955.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="J8V14_000832"
     CDS             complement(12014..13294)
                     /locus_tag="J8V14_000832"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBA0920956.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="J8V14_000833"
     CDS             complement(13313..14239)
                     /locus_tag="J8V14_000833"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBA0920957.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="J8V14_000834"
     CDS             complement(14252..15535)
                     /locus_tag="J8V14_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBA0920958.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="J8V14_000835"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="J8V14_000835"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBA0920959.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="J8V14_000836"
     CDS             complement(16323..17234)
                     /locus_tag="J8V14_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBA0920960.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="J8V14_000837"
     CDS             complement(17231..17695)
                     /locus_tag="J8V14_000837"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBA0920961.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="J8V14_000838"
     CDS             complement(17700..18398)
                     /locus_tag="J8V14_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBA0920962.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="J8V14_000839"
     CDS             complement(18385..18771)
                     /locus_tag="J8V14_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920963.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="J8V14_000840"
     CDS             complement(18918..20588)
                     /locus_tag="J8V14_000840"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBA0920964.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="J8V14_000841"
     CDS             20771..21004
                     /locus_tag="J8V14_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBA0920965.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="J8V14_000842"
     CDS             complement(21103..21534)
                     /locus_tag="J8V14_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBA0920966.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="J8V14_000843"
     CDS             21613..22026
                     /locus_tag="J8V14_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBA0920967.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="J8V14_000844"
     CDS             complement(22064..23212)
                     /locus_tag="J8V14_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBA0920968.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="J8V14_000845"
     CDS             complement(23334..23930)
                     /locus_tag="J8V14_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA0920969.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="J8V14_000846"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="J8V14_000846"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBA0920970.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="J8V14_000847"
     CDS             27160..27591
                     /locus_tag="J8V14_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0920971.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="J8V14_000848"
     CDS             complement(27636..28985)
                     /locus_tag="J8V14_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA0920972.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="J8V14_000849"
     CDS             complement(29055..30218)
                     /locus_tag="J8V14_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920973.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="J8V14_000850"
     CDS             30347..30670
                     /locus_tag="J8V14_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920974.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="J8V14_000851"
     CDS             complement(30853..32202)
                     /locus_tag="J8V14_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0920975.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="J8V14_000852"
     CDS             complement(32271..33224)
                     /locus_tag="J8V14_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBA0920976.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="J8V14_000853"
     CDS             complement(33326..34516)
                     /locus_tag="J8V14_000853"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBA0920977.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9945 a         7634 c         6116 g        11237 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aa
//
LOCUS       DADQRX010000018        34879 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.018, whole genome shotgun sequence.
ACCESSION   DADQRX010000018 DADQRX010000000
VERSION     DADQRX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.018"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(323..1171)
                     /locus_tag="J8V14_000854"
     CDS             complement(323..1171)
                     /locus_tag="J8V14_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA0920978.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1395..3335)
                     /gene="thrS"
                     /locus_tag="J8V14_000855"
     CDS             complement(1395..3335)
                     /gene="thrS"
                     /locus_tag="J8V14_000855"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBA0920979.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3672..3851)
                     /locus_tag="J8V14_000856"
     CDS             complement(3672..3851)
                     /locus_tag="J8V14_000856"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBA0920980.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3898..5721)
                     /gene="cadA"
                     /locus_tag="J8V14_000857"
     CDS             complement(3898..5721)
                     /gene="cadA"
                     /locus_tag="J8V14_000857"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBA0920981.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5999..8278
                     /locus_tag="J8V14_000858"
     CDS             5999..8278
                     /locus_tag="J8V14_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0920982.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8371..9018)
                     /locus_tag="J8V14_000859"
     CDS             complement(8371..9018)
                     /locus_tag="J8V14_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0920983.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9197..9916)
                     /locus_tag="J8V14_000860"
     CDS             complement(9197..9916)
                     /locus_tag="J8V14_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA0920984.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(10047..11198)
                     /locus_tag="J8V14_000861"
     CDS             complement(10047..11198)
                     /locus_tag="J8V14_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBA0920985.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11415..12155)
                     /locus_tag="J8V14_000862"
     CDS             complement(11415..12155)
                     /locus_tag="J8V14_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0920986.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12159..12482)
                     /gene="rsfS"
                     /locus_tag="J8V14_000863"
     CDS             complement(12159..12482)
                     /gene="rsfS"
                     /locus_tag="J8V14_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBA0920987.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12539..13132)
                     /locus_tag="J8V14_000864"
     CDS             complement(12539..13132)
                     /locus_tag="J8V14_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBA0920988.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13116..13763)
                     /locus_tag="J8V14_000865"
     CDS             complement(13116..13763)
                     /locus_tag="J8V14_000865"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBA0920989.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13776..14087)
                     /gene="yhbY"
                     /locus_tag="J8V14_000866"
     CDS             complement(13776..14087)
                     /gene="yhbY"
                     /locus_tag="J8V14_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBA0920990.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14103..15212)
                     /gene="yqeH"
                     /locus_tag="J8V14_000867"
     CDS             complement(14103..15212)
                     /gene="yqeH"
                     /locus_tag="J8V14_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBA0920991.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15209..15736)
                     /locus_tag="J8V14_000868"
     CDS             complement(15209..15736)
                     /locus_tag="J8V14_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBA0920992.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15916..16701)
                     /locus_tag="J8V14_000869"
     CDS             complement(15916..16701)
                     /locus_tag="J8V14_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBA0920993.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16694..17563)
                     /locus_tag="J8V14_000870"
     CDS             complement(16694..17563)
                     /locus_tag="J8V14_000870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBA0920994.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17564..18943)
                     /locus_tag="J8V14_000871"
     CDS             complement(17564..18943)
                     /locus_tag="J8V14_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBA0920995.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18951..19370)
                     /gene="fabZ"
                     /locus_tag="J8V14_000872"
     CDS             complement(18951..19370)
                     /gene="fabZ"
                     /locus_tag="J8V14_000872"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBA0920996.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19395..19871)
                     /gene="accB"
                     /locus_tag="J8V14_000873"
     CDS             complement(19395..19871)
                     /gene="accB"
                     /locus_tag="J8V14_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBA0920997.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19874..21109)
                     /gene="fabF"
                     /locus_tag="J8V14_000874"
     CDS             complement(19874..21109)
                     /gene="fabF"
                     /locus_tag="J8V14_000874"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBA0920998.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21128..21865)
                     /gene="fabG"
                     /locus_tag="J8V14_000875"
     CDS             complement(21128..21865)
                     /gene="fabG"
                     /locus_tag="J8V14_000875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBA0920999.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21877..22794)
                     /gene="fabD"
                     /locus_tag="J8V14_000876"
     CDS             complement(21877..22794)
                     /gene="fabD"
                     /locus_tag="J8V14_000876"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBA0921000.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22901..23125)
                     /locus_tag="J8V14_000877"
     CDS             complement(22901..23125)
                     /locus_tag="J8V14_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA0921001.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23178..24140)
                     /locus_tag="J8V14_000878"
     CDS             complement(23178..24140)
                     /locus_tag="J8V14_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBA0921002.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24150..24590)
                     /locus_tag="J8V14_000879"
     CDS             complement(24150..24590)
                     /locus_tag="J8V14_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0921003.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24896..25861
                     /locus_tag="J8V14_000880"
     CDS             24896..25861
                     /locus_tag="J8V14_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBA0921004.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25918..26148)
                     /locus_tag="J8V14_000881"
     CDS             complement(25918..26148)
                     /locus_tag="J8V14_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBA0921005.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26234..27121)
                     /locus_tag="J8V14_000882"
     CDS             complement(26234..27121)
                     /locus_tag="J8V14_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA0921006.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27210..28433)
                     /locus_tag="J8V14_000883"
     CDS             complement(27210..28433)
                     /locus_tag="J8V14_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA0921007.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28450..29529)
                     /locus_tag="J8V14_000884"
     CDS             complement(28450..29529)
                     /locus_tag="J8V14_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA0921008.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29660..31387)
                     /gene="ptsP"
                     /locus_tag="J8V14_000885"
     CDS             complement(29660..31387)
                     /gene="ptsP"
                     /locus_tag="J8V14_000885"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA0921009.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31387..31653)
                     /locus_tag="J8V14_000886"
     CDS             complement(31387..31653)
                     /locus_tag="J8V14_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBA0921010.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32132..34366
                     /locus_tag="J8V14_000887"
     CDS             32132..34366
                     /locus_tag="J8V14_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HBA0921011.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34514..34834
                     /locus_tag="J8V14_000888"
     CDS             34514..34834
                     /locus_tag="J8V14_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBA0921012.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9871 a         7531 c         6066 g        11411 t
ORIGIN      
        1 aatatttttt tcataaaatc tttcatcatt tcctatcctt ccgcctattt acattcatgc
       61 ttcattgtac ccaaaaactt cttaataaaa aagaaaaaat gggcggagca agaaattctt
      121 tacctaattt caagataaaa tcaacttttt gtttcacagc aatcacttaa agttacatat
      181 ccaactattt ttttcattaa aaaataaact tatttttgaa aattatgttt tcttaagtcg
      241 tgaaacactt tgatggatca tttatttgtg gaaaactacg ttttgttatt ctaaaaaact
      301 tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg attgcttctc
      361 gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa taaaaaatga
      421 tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga ttcgtgattt
      481 cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg ttatttccaa
      541 attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct aatgcgacta
      601 catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc tcattgctga
      661 caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta ccgacacgag
      721 accatttttg cagaatatct tctactacta aagaagaggc gccaacccca tataaaaaaa
      781 cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct tctagaatat
      841 ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg atagagtaaa
      901 aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt agttctgttg
      961 aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa
     1021 ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt gtagggacct
     1081 tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa taatcttcca
     1141 tcatactttc aatacgacta attacgctca taagttcctc caccctttta atgatagtct
     1201 atgtaagtat accatatcag acccttattc acggtcgtat actggataaa aaacaaaaaa
     1261 acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt gagcatctgt
     1321 gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact acttttttac
     1381 aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc catagcatca
     1441 ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg tacgttcact
     1501 gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt ttgtgtttga
     1561 gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac acgcaacccc
     1621 ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc aacagaaaca
     1681 gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt gtatacttct
     1741 gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat aactactgga
     1801 cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc tggcaataag
     1861 aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt gacttgaaca
     1921 tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc tagttctaat
     1981 tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc atcatcaaaa
     2041 tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc tgtaatattg
     2101 aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc tttgatttga
     2161 tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg ttgtaaacct
     2221 gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat acgaattggt
     2281 aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg gcagttcatt
     2341 gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat gtcttcttga
     2401 taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat tggtgtatat
     2461 acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat cgtgcgtcgg
     2521 attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg ggaaaccatg
     2581 aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc ttcacggaga
     2641 cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac acgttgcatc
     2701 atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa tttaaatact
     2761 tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt
     2821 tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac tttgtacgga
     2881 tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga
     2941 ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc tgcagttact
     3001 tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg tccaacgcca
     3061 aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga
     3121 cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact tgcatcttcc
     3181 tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa agcttttttt
     3241 gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga ctcaaattct
     3301 ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt gaaatgtttt
     3361 gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg aaaagagata
     3421 ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt
     3481 tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat cctttggttt
     3541 tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc aaattcagtt
     3601 ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa aaatcaagtg
     3661 ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt tttcatctca
     3721 ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg
     3781 atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa ttcgacatgt
     3841 acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc gattctatca
     3901 tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa
     3961 ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga ataatgcggg
     4021 gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc caatcagtac
     4081 agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg ctacacgcaa
     4141 gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag aaccatgagc
     4201 ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc
     4261 taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca gtttatcttg
     4321 aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg tttgagcaat
     4381 ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc ttagctcctt
     4441 gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga
     4501 tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa
     4561 agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc cttgaccggt
     4621 cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt tcactaacga
     4681 acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa gtagctcttc
     4741 tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt tggtaagcgt
     4801 tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt
     4861 ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag caatgggcgc
     4921 agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc
     4981 ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc
     5041 ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt
     5101 aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc
     5161 gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga
     5221 ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc
     5281 gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac
     5341 tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa
     5401 agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt
     5461 aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat
     5521 tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc
     5581 caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc
     5641 cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa
     5701 aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt
     5761 aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa
     5821 caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta
     5881 caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt
     5941 tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat
     6001 gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga
     6061 acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa
     6121 agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt
     6181 tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca
     6241 aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt
     6301 taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc
     6361 agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga
     6421 gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa
     6481 acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat
     6541 caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat
     6601 cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat
     6661 gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt
     6721 tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa
     6781 ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc
     6841 tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga
     6901 acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag
     6961 cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc
     7021 cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca
     7081 atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac
     7141 gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa
     7201 agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac
     7261 agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt
     7321 ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct
     7381 ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag
     7441 gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga
     7501 ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa
     7561 agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct
     7621 gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt
     7681 tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg
     7741 tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt
     7801 tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat
     7861 ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca
     7921 gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc
     7981 tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag
     8041 tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt
     8101 tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt
     8161 gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa
     8221 tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt
     8281 aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa taataattgc
     8341 agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca aatctgtttc
     8401 aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt tttctggata
     8461 ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca atcggtaaat
     8521 tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat agccgccaca
     8581 atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt tatttttttt
     8641 cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt tcaagctcca
     8701 ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc gaaaggctga
     8761 atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca gcattggtgc
     8821 gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat agtttcctaa
     8881 tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac aatcagttac
     8941 gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa aaggttctaa
     9001 agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat atcatttcac
     9061 atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata aatcacacaa
     9121 aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt
     9181 cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt
     9241 ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg
     9301 ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt
     9361 cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc
     9421 cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct
     9481 cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg
     9541 ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg
     9601 ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc
     9661 cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc
     9721 ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt
     9781 tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa
     9841 attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg
     9901 cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa
     9961 cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt
    10021 cattcttctc atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt
    10081 tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct
    10141 tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat
    10201 cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga
    10261 atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat
    10321 gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct
    10381 gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc
    10441 aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc
    10501 gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct
    10561 tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt
    10621 tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg
    10681 gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta
    10741 atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga
    10801 tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg
    10861 aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc
    10921 agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat
    10981 tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc
    11041 catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg
    11101 acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg tccattgtga
    11161 aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat
    11221 cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact
    11281 atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa
    11341 tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg
    11401 agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat
    11461 gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc
    11521 attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata
    11581 aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt
    11641 ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag
    11701 ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa taaaaatcgg
    11761 ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt
    11821 ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat
    11881 aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg
    11941 ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt
    12001 gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct
    12061 tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc gtccatcact
    12121 tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga
    12181 tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg ttctggcgca
    12241 tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt
    12301 ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta
    12361 attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag
    12421 gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca
    12481 atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt
    12541 atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt
    12601 gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag
    12661 cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt
    12721 ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc
    12781 ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca aacgaagcgc
    12841 caacaagacc atgccagatt gcattattat aattcaataa ctccggatca aaaccgtctc
    12901 ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg
    12961 cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt
    13021 cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca
    13081 tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc atacaaccct
    13141 tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga acagccttct
    13201 ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt
    13261 acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata
    13321 gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata
    13381 ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg
    13441 taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc
    13501 accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca
    13561 tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct
    13621 tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct
    13681 acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca
    13741 aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat
    13801 agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt
    13861 tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca
    13921 tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt
    13981 tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga
    14041 tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt
    14101 ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt
    14161 actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct
    14221 ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga
    14281 ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt
    14341 cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata
    14401 aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact
    14461 ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc
    14521 gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa
    14581 ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt
    14641 actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact
    14701 ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc
    14761 atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg
    14821 taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata
    14881 tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg
    14941 attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttttgc
    15001 cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttgggtctca
    15061 ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt
    15121 tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca
    15181 ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag
    15241 ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct
    15301 tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg
    15361 ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat
    15421 ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat
    15481 acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa
    15541 caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc
    15601 catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc
    15661 ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt
    15721 tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact
    15781 cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct
    15841 ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt
    15901 agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca gtaatttatg
    15961 ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc
    16021 gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc gatcaacgat
    16081 tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg
    16141 actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag cataaatcgt
    16201 ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc cacttccacc
    16261 ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga gcagatttct
    16321 ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt
    16381 attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca gtcgtaaagc
    16441 ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt cctgcagatc
    16501 ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag cgattccacc
    16561 aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga aagaatcgaa
    16621 gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta actgaacgac
    16681 atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat tcaagaagcg
    16741 tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa ccatgagata
    16801 ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc tgttcgatga
    16861 cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata tcgccttcca
    16921 ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt agataaaaaa
    16981 gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc attagagaaa
    17041 agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact ggaagacttt
    17101 cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt cccatacttc
    17161 ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga atggttgctt
    17221 taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta attttttccg
    17281 tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat tcttcaaaac
    17341 ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa gcgtatccgc
    17401 aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg
    17461 gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg ctctctctat
    17521 taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg cctcctatct
    17581 ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac
    17641 ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc
    17701 cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca aaacgcgtat
    17761 ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc gtatatcccg
    17821 ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt aagttttgga
    17881 tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc cggcattcga
    17941 ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca ccggctgcta
    18001 tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact ggatgttcca
    18061 cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact
    18121 cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca gcgtctccta
    18181 gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt actttttgat
    18241 tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca aatcgatcac
    18301 ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa tacatatcat
    18361 catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt gggagctctt
    18421 cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct ttcagcatca
    18481 ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat aatacaccag
    18541 tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca ttgatcttat
    18601 ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg cactcttcac
    18661 acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga attgcttcgg
    18721 cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa tcaacggctt
    18781 ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct tcggcatctg
    18841 cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga atgatacgta
    18901 cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac ctatccaatc
    18961 ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat tcctttgcct
    19021 attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt atctcccggt
    19081 gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt ctttcccttg
    19141 aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc aacaatcaat
    19201 acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt
    19261 acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg atcaatcaaa
    19321 agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat tgttttccct
    19381 ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata ttcgacaact
    19441 gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt tatctcattc
    19501 atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg atcacctact
    19561 tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac gattggcgaa
    19621 acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc tggagcagtt
    19681 gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc gtttgtttga
    19741 accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa ttgaccttca
    19801 cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc tctgatttca
    19861 ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa
    19921 accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg tttttgacaa
    19981 ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg ataaattgat
    20041 gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc aataaatgtc
    20101 cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct aatgcgattt
    20161 ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga gcattgatat
    20221 atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt gctttagatg
    20281 caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt gcaccgtatc
    20341 caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa
    20401 ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca aacggaatcg
    20461 aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat ccagcaatcc
    20521 ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa tagccatgtt
    20581 tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct gttacgatag
    20641 ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc atgttggcaa
    20701 tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt agacgaataa
    20761 cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg ccaaaccggt
    20821 cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga
    20881 ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa tatttatcgt
    20941 aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca tcaaatttag
    21001 tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg tctgcgtcat
    21061 ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt tgtttgctct
    21121 ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt ccggtgatat
    21181 atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt ccgaaacgct
    21241 gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact tccgtcatat
    21301 cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt gcagcttctc
    21361 gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa tttgcttgac
    21421 caatatttcc aatcaaccca gatacactag agagattaat gatggcccct tctcgctgct
    21481 tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca
    21541 gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt gtgattccgg
    21601 cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg atcatttgtc
    21661 ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca ccgaatgctt
    21721 cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg atatttgctc
    21781 cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta cctgtgataa
    21841 atacattttt tccttttaca tccataacgt tcctccttat agtattgcta ttgtttcatc
    21901 aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt ttttcataaa
    21961 tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga ttgtttttaa
    22021 cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc
    22081 gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa aagaaggttc
    22141 agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt ctaaaatagc
    22201 tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt tttcttttaa
    22261 tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct gctgaggtgt
    22321 attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc tttcctcgat
    22381 cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg gggcagcttc
    22441 tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg atagcgcatt
    22501 actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat ctgggatgat
    22561 tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag ctggctgtgt
    22621 atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca tatcgtagcc
    22681 gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat ataaatcttt
    22741 tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt tcatgttttt
    22801 ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg agacattcct
    22861 atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt tgtttttcta
    22921 cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa
    22981 attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct tctaagtctt
    23041 cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct tctgcgatga
    23101 tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt tgctttttat
    23161 ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa acctccgcca
    23221 tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact agaaactgct
    23281 tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc catattggtc
    23341 aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat tctgaggttt
    23401 gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg gatattgttt
    23461 gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa ataatacgaa
    23521 ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc ggtcaatgag
    23581 ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg tggattcgct
    23641 tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg atcagaccaa
    23701 tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga gccgcagcgg
    23761 atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc actgatatca
    23821 aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac agatggtgtc
    23881 gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg gtttagattt
    23941 ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc ctgatttgtt
    24001 acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga ggtatccatc
    24061 atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc tgttgcagta
    24121 atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa gtcgtgcaag
    24181 tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc taagatgttc
    24241 ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc ttttttcgtt
    24301 agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac atagcctttt
    24361 tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag ttctttggct
    24421 acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg catctctgtg
    24481 attgacagat ctttgaattg agattttttc agctcagact cttcgattgt caaaatatca
    24541 ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat gttccaccgt
    24601 ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta acttttttca
    24661 aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga ttttactcat
    24721 ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc atctatctca
    24781 catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt
    24841 ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt
    24901 aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga
    24961 tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt
    25021 tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt
    25081 tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca
    25141 acattaccgt cctttttata accgacaaat gaattatttc agtaattata attatgtaca
    25201 tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt
    25261 tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc
    25321 ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat
    25381 gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct
    25441 actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc
    25501 cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca
    25561 aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag
    25621 tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg
    25681 tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca
    25741 attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga
    25801 aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta
    25861 gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta
    25921 ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg
    25981 atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca
    26041 cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac
    26101 catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc
    26161 ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa
    26221 aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga
    26281 tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca
    26341 atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct
    26401 ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac
    26461 gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca
    26521 ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca
    26581 tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac
    26641 catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg
    26701 cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg
    26761 ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg
    26821 ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa
    26881 aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa
    26941 agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg
    27001 ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt
    27061 ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca
    27121 ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca
    27181 aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg
    27241 attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc
    27301 atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt
    27361 ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct
    27421 ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg
    27481 ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa
    27541 gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa
    27601 agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt
    27661 tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg
    27721 gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc
    27781 tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac
    27841 tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg
    27901 ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata
    27961 acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat
    28021 tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac
    28081 atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt
    28141 atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc
    28201 aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt
    28261 acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta
    28321 aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta
    28381 gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc
    28441 gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt
    28501 cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt
    28561 tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat
    28621 cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca
    28681 gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa
    28741 aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata
    28801 cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc
    28861 ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca
    28921 aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg
    28981 ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc
    29041 tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga
    29101 aatagcttga atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt
    29161 tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg
    29221 agaaagctgg ttcgatccga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg
    29281 atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc
    29341 gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc
    29401 gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag
    29461 catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata
    29521 gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact
    29581 gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca
    29641 ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca
    29701 tcgtatcgca ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc
    29761 gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac
    29821 ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg
    29881 cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg
    29941 ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt
    30001 attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg
    30061 ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt
    30121 ccttgccttc acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga
    30181 attctttgag tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac
    30241 gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac
    30301 gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt
    30361 ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta
    30421 atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat
    30481 acaggaattc agtacggtac agaccaactg cttctccacc attattatgg acaccaacta
    30541 gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag
    30601 tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt
    30661 tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt
    30721 tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg
    30781 ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg
    30841 ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat
    30901 gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata
    30961 agtgcgctaa aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag
    31021 cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg
    31081 attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat
    31141 ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg
    31201 ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac
    31261 gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa
    31321 ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt
    31381 caaccattat tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc
    31441 agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga
    31501 cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc
    31561 agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt
    31621 ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt
    31681 tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct
    31741 ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag
    31801 ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa
    31861 acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct
    31921 ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata
    31981 ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct
    32041 cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt
    32101 acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga
    32161 ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca
    32221 gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa
    32281 tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca
    32341 acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa
    32401 tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat
    32461 cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa
    32521 acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc
    32581 aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt
    32641 accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg
    32701 tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca
    32761 agcagaaaat gtgatcctat tcatagatga agttcacgaa atagtcggtg ctggttctgc
    32821 tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt
    32881 gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt
    32941 agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat
    33001 tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc
    33061 catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa
    33121 agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga
    33181 tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg
    33241 agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat
    33301 gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa
    33361 aatcgttgaa caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca
    33421 actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga
    33481 tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat
    33541 cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact
    33601 tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat
    33661 ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga
    33721 agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga
    33781 agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg
    33841 attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag
    33901 taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt
    33961 tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt
    34021 tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact
    34081 aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac
    34141 gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct
    34201 tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc
    34261 ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa
    34321 accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag
    34381 cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata
    34441 ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa
    34501 agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa
    34561 ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa ataccactat
    34621 tgtctacact gaagcccctc aacataatga aattctgctg atcaatgaca aacgtaaaat
    34681 ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt
    34741 ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc
    34801 aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag
    34861 agcgtgggac aaaagtaaa
//
LOCUS       DADQRX010000019        32497 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.019, whole genome shotgun sequence.
ACCESSION   DADQRX010000019 DADQRX010000000
VERSION     DADQRX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.019"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="J8V14_000889"
     CDS             complement(<1..563)
                     /locus_tag="J8V14_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBA0921013.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="J8V14_000890"
     CDS             complement(750..1400)
                     /locus_tag="J8V14_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921014.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="J8V14_000891"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="J8V14_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBA0921015.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="J8V14_000892"
     CDS             complement(2912..3910)
                     /locus_tag="J8V14_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBA0921016.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="J8V14_000893"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="J8V14_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBA0921017.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="J8V14_000894"
     CDS             complement(4926..5657)
                     /locus_tag="J8V14_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBA0921018.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="J8V14_000895"
     CDS             complement(5661..6926)
                     /locus_tag="J8V14_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBA0921019.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="J8V14_000896"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="J8V14_000896"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBA0921020.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="J8V14_000897"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="J8V14_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBA0921021.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="J8V14_000898"
     CDS             12268..13770
                     /locus_tag="J8V14_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0921022.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="J8V14_000899"
     CDS             13772..14509
                     /locus_tag="J8V14_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA0921023.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            14515..14673
                     /locus_tag="J8V14_000900"
                     /pseudo
     CDS             14515..14673
                     /locus_tag="J8V14_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(14680..15873)
                     /locus_tag="J8V14_000901"
     CDS             complement(14680..15873)
                     /locus_tag="J8V14_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0921024.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="J8V14_000902"
     CDS             complement(15952..16158)
                     /locus_tag="J8V14_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA0921025.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="J8V14_000903"
     CDS             complement(16151..17293)
                     /locus_tag="J8V14_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBA0921026.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="J8V14_000904"
     CDS             17805..18440
                     /locus_tag="J8V14_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921027.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="J8V14_000905"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="J8V14_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="J8V14_000906"
     CDS             18916..19032
                     /locus_tag="J8V14_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBA0921028.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="J8V14_000907"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="J8V14_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBA0921029.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="J8V14_000908"
     CDS             21086..21253
                     /locus_tag="J8V14_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0921030.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="J8V14_000909"
     CDS             complement(21313..21666)
                     /locus_tag="J8V14_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921031.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="J8V14_000910"
     CDS             22170..22589
                     /locus_tag="J8V14_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBA0921032.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="J8V14_000911"
     CDS             22615..22821
                     /locus_tag="J8V14_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921033.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="J8V14_000912"
     CDS             complement(23049..23222)
                     /locus_tag="J8V14_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBA0921034.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="J8V14_000913"
     CDS             23198..23863
                     /locus_tag="J8V14_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HBA0921035.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="J8V14_000914"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="J8V14_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBA0921036.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="J8V14_000915"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="J8V14_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBA0921037.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="J8V14_000916"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="J8V14_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBA0921038.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="J8V14_000917"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="J8V14_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBA0921039.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="J8V14_000918"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="J8V14_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="J8V14_000919"
     CDS             complement(30257..30484)
                     /locus_tag="J8V14_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBA0921040.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="J8V14_000920"
     CDS             30968..31558
                     /locus_tag="J8V14_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBA0921041.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31748..32461
                     /locus_tag="J8V14_000921"
     CDS             31748..32461
                     /locus_tag="J8V14_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921042.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DADQRX010000020        32322 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.020, whole genome shotgun sequence.
ACCESSION   DADQRX010000020 DADQRX010000000
VERSION     DADQRX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.020"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..801
                     /gene="truA"
                     /locus_tag="J8V14_000922"
     CDS             55..801
                     /gene="truA"
                     /locus_tag="J8V14_000922"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBA0921043.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1256..3925)
                     /locus_tag="J8V14_000923"
     CDS             complement(1256..3925)
                     /locus_tag="J8V14_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBA0921044.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4163..4351)
                     /locus_tag="J8V14_000924"
     CDS             complement(4163..4351)
                     /locus_tag="J8V14_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBA0921045.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4621..4983
                     /locus_tag="J8V14_000925"
     CDS             4621..4983
                     /locus_tag="J8V14_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBA0921046.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5035..6717
                     /locus_tag="J8V14_000926"
     CDS             5035..6717
                     /locus_tag="J8V14_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBA0921047.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6834..8870
                     /gene="recG"
                     /locus_tag="J8V14_000927"
     CDS             6834..8870
                     /gene="recG"
                     /locus_tag="J8V14_000927"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBA0921048.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8919..9920
                     /gene="plsX"
                     /locus_tag="J8V14_000928"
     CDS             8919..9920
                     /gene="plsX"
                     /locus_tag="J8V14_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBA0921049.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10042..10284
                     /gene="acpP"
                     /locus_tag="J8V14_000929"
     CDS             10042..10284
                     /gene="acpP"
                     /locus_tag="J8V14_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA0921050.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10552..11556
                     /locus_tag="J8V14_000930"
     CDS             10552..11556
                     /locus_tag="J8V14_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921051.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11557..12501
                     /locus_tag="J8V14_000931"
     CDS             11557..12501
                     /locus_tag="J8V14_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921052.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12501..13463
                     /locus_tag="J8V14_000932"
     CDS             12501..13463
                     /locus_tag="J8V14_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921053.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13490..14404
                     /locus_tag="J8V14_000933"
     CDS             13490..14404
                     /locus_tag="J8V14_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921054.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14430..16211
                     /locus_tag="J8V14_000934"
     CDS             14430..16211
                     /locus_tag="J8V14_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0921055.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16559..17245
                     /locus_tag="J8V14_000935"
     CDS             16559..17245
                     /locus_tag="J8V14_000935"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBA0921056.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17265..20846
                     /gene="smc"
                     /locus_tag="J8V14_000936"
     CDS             17265..20846
                     /gene="smc"
                     /locus_tag="J8V14_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBA0921057.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20855..21664
                     /locus_tag="J8V14_000937"
     CDS             20855..21664
                     /locus_tag="J8V14_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0921058.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21675..22673
                     /gene="ftsY"
                     /locus_tag="J8V14_000938"
     CDS             21675..22673
                     /gene="ftsY"
                     /locus_tag="J8V14_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBA0921059.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22860..25127
                     /gene="gshAB"
                     /locus_tag="J8V14_000939"
     CDS             22860..25127
                     /gene="gshAB"
                     /locus_tag="J8V14_000939"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBA0921060.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25216..25668)
                     /locus_tag="J8V14_000940"
     CDS             complement(25216..25668)
                     /locus_tag="J8V14_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBA0921061.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25818..26768
                     /locus_tag="J8V14_000941"
     CDS             25818..26768
                     /locus_tag="J8V14_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBA0921062.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26761..27720
                     /locus_tag="J8V14_000942"
     CDS             26761..27720
                     /locus_tag="J8V14_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBA0921063.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27717..28472
                     /locus_tag="J8V14_000943"
     CDS             27717..28472
                     /locus_tag="J8V14_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0921064.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28495..29448
                     /locus_tag="J8V14_000944"
     CDS             28495..29448
                     /locus_tag="J8V14_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0921065.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30092..30388)
                     /locus_tag="J8V14_000945"
     CDS             complement(30092..30388)
                     /locus_tag="J8V14_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBA0921066.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30743..31099
                     /locus_tag="J8V14_000946"
     CDS             30743..31099
                     /locus_tag="J8V14_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBA0921067.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31109..32008
                     /locus_tag="J8V14_000947"
     CDS             31109..32008
                     /locus_tag="J8V14_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBA0921068.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10708 a         5732 c         6906 g         8976 t
ORIGIN      
        1 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
       61 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
      121 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
      181 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
      241 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
      301 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
      361 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
      421 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
      481 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
      541 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
      601 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
      661 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
      721 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
      781 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
      841 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
      901 tacttctgga acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta
      961 gagaaacaaa cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg
     1021 tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
     1081 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
     1141 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
     1201 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
     1261 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
     1321 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
     1381 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
     1441 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
     1501 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
     1561 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
     1621 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
     1681 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
     1741 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
     1801 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
     1861 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
     1921 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
     1981 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
     2041 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
     2101 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
     2161 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
     2221 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
     2281 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
     2341 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
     2401 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
     2461 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
     2521 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
     2581 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
     2641 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
     2701 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
     2761 ccaaaatgat tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc
     2821 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
     2881 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
     2941 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
     3001 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
     3061 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
     3121 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
     3181 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
     3241 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
     3301 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
     3361 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
     3421 gaagataaaa aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
     3481 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
     3541 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
     3601 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
     3661 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
     3721 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
     3781 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
     3841 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
     3901 agaatgactt taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt
     3961 cagtttatca tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata
     4021 aaatttagaa taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg
     4081 gtaggaatca aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct
     4141 gaacggtcca agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag
     4201 ttgacaccca aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag
     4261 gtttaacagt acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct
     4321 tgcgacctgt aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga
     4381 gcaatagaat aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag
     4441 tacattttca tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat
     4501 acctgaaatt ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg
     4561 ctatgataaa atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc
     4621 atggctgtaa aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca
     4681 acagttgttg gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat
     4741 caaattaaag ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt
     4801 gtccgccaag aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg
     4861 aaaatctctg aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta
     4921 ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac
     4981 taggcaggag actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt
     5041 gtaacagaaa taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg
     5101 caagtaaatg cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg
     5161 ggaacaaata tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca
     5221 gaaaaagtag gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga
     5281 aattccggcg ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt
     5341 gtctcattaa atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat
     5401 aaagcagtaa tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa
     5461 gctggagaaa agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta
     5521 aaaggagcaa agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc
     5581 ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca
     5641 ttgaatggag aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat
     5701 atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat
     5761 tttggttact gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca
     5821 ttcgattacg aagaattccg taactattta gatggtatag gagattcttt acttgtggtc
     5881 aatgatgacg agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat
     5941 tatggacaaa aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat
     6001 gagacgattt tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt
     6061 atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt
     6121 ttaggcgcta attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt
     6181 gttaaagcga ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac
     6241 atcttcatgg cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct
     6301 tcaagaacga tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta
     6361 gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact
     6421 cacgcgatca gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc
     6481 atgatcgatg gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca
     6541 ctagaacaaa tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga
     6601 acagttgaac aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa
     6661 atcgagatcc accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt
     6721 ctgtgtcaga atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc
     6781 aggtatgatc gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt
     6841 ctttagatga tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg
     6901 ccagcttggg catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag
     6961 atattcagga aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa
     7021 tcgtagtctc gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc
     7081 gtatgatgca agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag
     7141 aaaaagtcat tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag
     7201 ccttgaacgg gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc
     7261 acgtcaacaa atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag
     7321 aatacggcac attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc
     7381 ttgaccgagc ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc
     7441 aagccaaacg acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat
     7501 tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat
     7561 tgaaacaatt tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga
     7621 atgaaatctg tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg
     7681 taggtagcgg aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt
     7741 tccaaggtgc gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc
     7801 aacagctgtt tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa
     7861 aagaacgacg tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca
     7921 cacatgcatt gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg
     7981 agcagcaccg ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag
     8041 atgtcttatt tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg
     8101 agatggatgt atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct
     8161 gggtaagaac acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag
     8221 gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa
     8281 atgcagtgga aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct
     8341 tgttacatgg taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca
     8401 accagatgca gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg
     8461 cgaccgtcat gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc
     8521 gtggacgtgt tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa
     8581 atgaactagg tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa
     8641 gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc
     8701 tgcctcaatt tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag
     8761 aagaggctgc agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt
     8821 tagcgcaaag cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc
     8881 tatactttat actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa
     8941 tgggcggaga tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt
     9001 ttccagaagt agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa
     9061 atgaaaaaaa catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag
     9121 taaaagcaat tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa
     9181 acggtgaagc agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt
     9241 tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag
     9301 tagtagggaa agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca
     9361 aaccagaaca tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag
     9421 gtatcggacg tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg
     9481 aagtgacgaa aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa
     9541 acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta
     9601 caggcaacgc tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa
     9661 aatctgctat tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg
     9721 gcttacgttc tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg
     9781 gcttgaaagc accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata
     9841 cgatccgcca aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat
     9901 ttgaaaaaag cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag
     9961 gtggacattc ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta
    10021 tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt
    10081 atttcaaatc attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat
    10141 gatttaaaag ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc
    10201 ggtacggaaa tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat
    10261 tatatcagca gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat
    10321 gcagcttttt atccttcttt ttttaatctt tttgacacgt aaaagtcatt tttctgataa
    10381 ttctggcgat aaacagaaat tatgttgcaa agtaaagcgg ttttctatat aattattaat
    10441 aattcattac acaaagataa gaataacctt tgttataaaa cgatttattc tgtgtaatgt
    10501 ccttttttat taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat
    10561 aaccaattat tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac
    10621 gatgcagtag atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg
    10681 gaatctgggt gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat
    10741 aacacaaaaa ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa
    10801 acgctctata ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct
    10861 gcattaaatc ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca
    10921 aagatgaatg cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt
    10981 cctaatcctg cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa
    11041 cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg
    11101 acgacagccc tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag
    11161 gaagaaacaa aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg
    11221 gctgatcgag tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa
    11281 ctgtttgatc atcctaagca tccttataca cgctctttac tgaactctat accacaagaa
    11341 ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg
    11401 cctcgtaaag gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa
    11461 gaagagccta tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag
    11521 catttccact ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa
    11581 gacctgaaag ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta
    11641 tatgctgtag atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga
    11701 gagtctggct ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca
    11761 gccggacaaa tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg
    11821 ggttataaca aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa
    11881 aagcgagtgt tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa
    11941 gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta
    12001 tataaatatc ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct
    12061 gttgctacca atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct
    12121 gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat
    12181 ttattcattt cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg
    12241 tacaaaggtc gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat
    12301 atctatacga aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc
    12361 cacaaagaaa atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat
    12421 gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg
    12481 aaagatggag gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc
    12541 cgcaagttat tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc
    12601 catttaccgg tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag
    12661 cagctggctt gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc
    12721 aaggcgattt tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac
    12781 ggatcatcaa cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg
    12841 tgcctctggg gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa
    12901 tctacaactt ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg
    12961 tttttggtta tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag
    13021 gtggatttat cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc
    13081 aagcattttt ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga
    13141 aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca
    13201 gtcgacatat tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca
    13261 cttcgttgat tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa
    13321 aactatttat tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga
    13381 ttttaggaat cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg
    13441 acccgcgtat tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa
    13501 aaacaataca gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga
    13561 atttaaaaaa gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt
    13621 gatctttatc ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga
    13681 caaatacgct gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga
    13741 tgaaggcgga cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat
    13801 cggatttgcg atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg
    13861 ctactacgga gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct
    13921 accgatcatg ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc
    13981 ctttatattc atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa
    14041 gacattatct gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga
    14101 ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt
    14161 gacgatgaac tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg
    14221 cttgccggca aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt
    14281 cttagtgaat cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt
    14341 gagtatcaat tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg
    14401 ctagtataga agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc
    14461 tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca
    14521 actctggagg caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata
    14581 atgatgatgc aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta
    14641 aaggattgtt ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt
    14701 ctcatgaacc gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa
    14761 cgcttgatct agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat
    14821 ggtcagatgg tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga
    14881 ataaagatta cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag
    14941 aatatcatga tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag
    15001 ttgaaatcga atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt
    15061 catatgcagc accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg
    15121 atcaagtacg taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag
    15181 gagaatcagt agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa
    15241 agatcacgat gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat
    15301 atgatatgat tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct
    15361 atacaaactt aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat
    15421 ggaatgcaga tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat
    15481 tacgtcaagc aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag
    15541 gtttgagaag caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg
    15601 agatgaaagg attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct
    15661 acaaagatgt ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca
    15721 actttgcttc gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac
    15781 aatggaaaga aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt
    15841 ctttctatga taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga
    15901 atacaggaac tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca
    15961 ctcgttatga atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat
    16021 ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag
    16081 catttgcgat tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag
    16141 actgggattg gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt
    16201 cacgttcata atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat
    16261 atttttctgt ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat
    16321 aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa
    16381 gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc
    16441 gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga
    16501 caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat
    16561 ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa
    16621 tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt
    16681 atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca
    16741 gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc
    16801 gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt
    16861 tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga
    16921 cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc
    16981 attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga
    17041 tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg
    17101 attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga
    17161 tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc
    17221 ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa
    17281 ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg
    17341 tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct
    17401 gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct
    17461 ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca
    17521 atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac
    17581 ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag
    17641 aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag
    17701 tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg
    17761 gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag
    17821 acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg
    17881 ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg
    17941 atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag
    18001 cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag
    18061 aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa
    18121 atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg
    18181 ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag
    18241 cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag
    18301 cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag
    18361 aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg
    18421 ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat
    18481 atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat
    18541 cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat
    18601 catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc
    18661 aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc
    18721 tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag
    18781 gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg
    18841 aactgatcga tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag
    18901 cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac
    18961 agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca agacaattac
    19021 cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac
    19081 aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt
    19141 tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag
    19201 tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata
    19261 aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg
    19321 aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac
    19381 aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac
    19441 gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt
    19501 ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag
    19561 attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac
    19621 aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa
    19681 gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc
    19741 agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg
    19801 aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg
    19861 aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat
    19921 taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata
    19981 agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat
    20041 ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc
    20101 tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg
    20161 atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac
    20221 cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag
    20281 caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga
    20341 tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca
    20401 aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa
    20461 ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa
    20521 gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca
    20581 ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg
    20641 aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta
    20701 tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga
    20761 tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg
    20821 cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt
    20881 ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc
    20941 acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat
    21001 ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa
    21061 cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt
    21121 atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt
    21181 gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt
    21241 gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt
    21301 gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat
    21361 accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt
    21421 tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt
    21481 gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata
    21541 tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt
    21601 cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga
    21661 gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga
    21721 aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa
    21781 catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt
    21841 ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa
    21901 tcaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc
    21961 aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa
    22021 atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg
    22081 tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag
    22141 tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg
    22201 gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg
    22261 tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg
    22321 caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa
    22381 tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg
    22441 gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg
    22501 ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat
    22561 tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg
    22621 atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt
    22681 ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca
    22741 tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt
    22801 cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa
    22861 tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc
    22921 gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg
    22981 atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg
    23041 aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc
    23101 tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat
    23161 taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa
    23221 atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga atacgggaag cgaaaacaga
    23281 tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca
    23341 gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag
    23401 cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga
    23461 gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca
    23521 acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac
    23581 agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat
    23641 tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg
    23701 ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc
    23761 cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag
    23821 aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac
    23881 aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc
    23941 aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc
    24001 aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga
    24061 gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc
    24121 cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg
    24181 ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc
    24241 aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata
    24301 tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt
    24361 tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga
    24421 gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca
    24481 cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc
    24541 gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg
    24601 tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca
    24661 gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga
    24721 atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag
    24781 aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga
    24841 actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt
    24901 acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt
    24961 tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg
    25021 aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa
    25081 gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt
    25141 tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt
    25201 atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc
    25261 tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc
    25321 atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta
    25381 atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg
    25441 gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc
    25501 ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata
    25561 gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt
    25621 accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct
    25681 ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat
    25741 gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa
    25801 ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt attttaagta
    25861 gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg
    25921 ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg
    25981 tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg
    26041 tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga
    26101 ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg
    26161 caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc
    26221 ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt
    26281 atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga
    26341 tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat
    26401 ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag
    26461 attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt
    26521 tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa
    26581 tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct
    26641 tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg
    26701 tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat
    26761 atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca
    26821 ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt
    26881 aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt
    26941 cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat
    27001 gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca
    27061 ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt
    27121 ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt
    27181 ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa
    27241 tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt
    27301 ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg
    27361 ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt
    27421 tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg
    27481 ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact
    27541 tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga
    27601 ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa
    27661 tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga
    27721 ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt
    27781 taacgattcc tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa
    27841 cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc
    27901 ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac
    27961 aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc
    28021 cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac
    28081 acattgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc
    28141 aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac
    28201 ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag
    28261 tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt
    28321 atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag
    28381 aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga
    28441 ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa
    28501 aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc
    28561 aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg
    28621 ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta
    28681 ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga
    28741 gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt
    28801 ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt
    28861 tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt
    28921 tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag
    28981 atctttggta agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat
    29041 cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa
    29101 gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc
    29161 tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa
    29221 tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt
    29281 ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt
    29341 aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta
    29401 gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag
    29461 aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc
    29521 ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg
    29581 gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt
    29641 tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg
    29701 cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata
    29761 tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata
    29821 agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    29881 acaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat
    29941 aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    30001 agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat
    30061 tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat
    30121 agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta
    30181 attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac
    30241 tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat
    30301 tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa
    30361 aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat
    30421 aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa
    30481 tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg
    30541 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
    30601 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
    30661 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
    30721 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
    30781 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
    30841 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
    30901 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
    30961 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
    31021 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
    31081 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
    31141 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
    31201 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
    31261 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
    31321 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
    31381 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
    31441 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
    31501 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
    31561 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
    31621 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
    31681 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
    31741 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
    31801 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
    31861 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
    31921 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
    31981 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
    32041 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
    32101 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
    32161 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
    32221 tttttattta attataatta atattgattg aagtctaggc agtcaagtcg agagtgtaaa
    32281 atattttgtg taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DADQRX010000021        32220 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.021, whole genome shotgun sequence.
ACCESSION   DADQRX010000021 DADQRX010000000
VERSION     DADQRX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.021"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(114..989)
                     /locus_tag="J8V14_000948"
     CDS             complement(114..989)
                     /locus_tag="J8V14_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBA0921069.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1098..2867)
                     /gene="aspS"
                     /locus_tag="J8V14_000949"
     CDS             complement(1098..2867)
                     /gene="aspS"
                     /locus_tag="J8V14_000949"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBA0921070.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2882..4174)
                     /locus_tag="J8V14_000950"
     CDS             complement(2882..4174)
                     /locus_tag="J8V14_000950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBA0921071.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4568..5515
                     /locus_tag="J8V14_000951"
     CDS             4568..5515
                     /locus_tag="J8V14_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA0921072.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5604..6863)
                     /locus_tag="J8V14_000952"
     CDS             complement(5604..6863)
                     /locus_tag="J8V14_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBA0921073.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7034..7480)
                     /locus_tag="J8V14_000953"
     CDS             complement(7034..7480)
                     /locus_tag="J8V14_000953"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBA0921074.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7491..9704)
                     /locus_tag="J8V14_000954"
     CDS             complement(7491..9704)
                     /locus_tag="J8V14_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBA0921075.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9937..10701)
                     /locus_tag="J8V14_000955"
     CDS             complement(9937..10701)
                     /locus_tag="J8V14_000955"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBA0921076.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10702..11649)
                     /gene="prmA"
                     /locus_tag="J8V14_000956"
     CDS             complement(10702..11649)
                     /gene="prmA"
                     /locus_tag="J8V14_000956"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBA0921077.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11662..12150)
                     /locus_tag="J8V14_000957"
     CDS             complement(11662..12150)
                     /locus_tag="J8V14_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBA0921078.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12116..12787)
                     /locus_tag="J8V14_000958"
     CDS             complement(12116..12787)
                     /locus_tag="J8V14_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBA0921079.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12925..14211
                     /locus_tag="J8V14_000959"
     CDS             12925..14211
                     /locus_tag="J8V14_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBA0921080.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14283..14477
                     /gene="ssrS"
                     /locus_tag="J8V14_000960"
     ncRNA           14283..14477
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="J8V14_000960"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14555..14839
                     /locus_tag="J8V14_000961"
     CDS             14555..14839
                     /locus_tag="J8V14_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921081.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14912..15391
                     /locus_tag="J8V14_000962"
     CDS             14912..15391
                     /locus_tag="J8V14_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBA0921082.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15614..15757
                     /locus_tag="J8V14_000963"
     CDS             15614..15757
                     /locus_tag="J8V14_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBA0921083.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16335..17918
                     /locus_tag="J8V14_000964"
     CDS             16335..17918
                     /locus_tag="J8V14_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBA0921084.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18355..19539)
                     /locus_tag="J8V14_000965"
     CDS             complement(18355..19539)
                     /locus_tag="J8V14_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBA0921085.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19564..20571)
                     /locus_tag="J8V14_000966"
     CDS             complement(19564..20571)
                     /locus_tag="J8V14_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0921086.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20743..21102)
                     /locus_tag="J8V14_000967"
     CDS             complement(20743..21102)
                     /locus_tag="J8V14_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921087.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21074..21445)
                     /locus_tag="J8V14_000968"
     CDS             complement(21074..21445)
                     /locus_tag="J8V14_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBA0921088.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21462..21764)
                     /locus_tag="J8V14_000969"
     CDS             complement(21462..21764)
                     /locus_tag="J8V14_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921089.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21781..22251)
                     /locus_tag="J8V14_000970"
     CDS             complement(21781..22251)
                     /locus_tag="J8V14_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBA0921090.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22226..22504)
                     /locus_tag="J8V14_000971"
     CDS             complement(22226..22504)
                     /locus_tag="J8V14_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBA0921091.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22501..23550)
                     /locus_tag="J8V14_000972"
     CDS             complement(22501..23550)
                     /locus_tag="J8V14_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBA0921092.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23507..24436)
                     /gene="cpaF"
                     /locus_tag="J8V14_000973"
     CDS             complement(23507..24436)
                     /gene="cpaF"
                     /locus_tag="J8V14_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HBA0921093.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24568..26322
                     /gene="ade"
                     /locus_tag="J8V14_000974"
     CDS             24568..26322
                     /gene="ade"
                     /locus_tag="J8V14_000974"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBA0921094.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26420..27082)
                     /gene="trmB"
                     /locus_tag="J8V14_000975"
     CDS             complement(26420..27082)
                     /gene="trmB"
                     /locus_tag="J8V14_000975"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBA0921095.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27152..27931)
                     /locus_tag="J8V14_000976"
     CDS             complement(27152..27931)
                     /locus_tag="J8V14_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBA0921096.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28138..29349)
                     /locus_tag="J8V14_000977"
     CDS             complement(28138..29349)
                     /locus_tag="J8V14_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921097.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29342..30079)
                     /locus_tag="J8V14_000978"
     CDS             complement(29342..30079)
                     /locus_tag="J8V14_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921098.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30217..30651
                     /locus_tag="J8V14_000979"
     CDS             30217..30651
                     /locus_tag="J8V14_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBA0921099.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30644..30988
                     /locus_tag="J8V14_000980"
     CDS             30644..30988
                     /locus_tag="J8V14_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBA0921100.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31168..32178
                     /locus_tag="J8V14_000981"
     CDS             31168..32178
                     /locus_tag="J8V14_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0921101.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         9641 a         6751 c         5747 g        10081 t
ORIGIN      
        1 atttcaaata aaaaagcagt ttttctttgc tgattctact atggtagaaa aaaactgcag
       61 acaaacgaac gtctgtctac agcagtattt catgaatttt tgtcaaactc gatctaaatg
      121 ccatcctcat agaaacgacc aagtatctgg acgttttctg caatacttac aaaagcttgt
      181 ggatcagaag ctttcattgc ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc
      241 atcaatactg tctgcttatc atgcttataa gcaccttctg cttcattgat gatcgtgatc
      301 cctctacgca tacagttttg gatttcattt actacttttt ctggatgttt cgttacgatc
      361 attacctgca tcttcttttg tttagtataa acgacatcag ttacttttcc actgacgaaa
      421 atagacaacg tactgtaaaa catatattgc cagccaaata gaattccggc aacaaaaacg
      481 atcaatgcat tgaagtagat cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc
      541 gtgatactca cgatatctag accgcccgat gataggccgt ttctcaacgc tagcccaata
      601 cctagtccca tcacagcccc cccaaacaac gcacagatga ttggatcggt agataggact
      661 gtttccggta aaatatggat acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt
      721 gtaaacttct tcccgatttt taaccacgcc aatacaaaca aaggtatatt cagtaaatac
      781 aaagttgttg ataccggtac ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc
      841 tgagctagac cagtaatacc acttgaataa atatgtcctg gctgataaaa gaaattcaat
      901 gcgactgatg cgatcaacgc ataaacgata gataccgaaa gcttggtcgt gtaatcatga
      961 taagcccaat tttcgaatgt ttttttcatg gaaaagcccc gctttatatg ttatggcttc
     1021 attataaagt gtttttcaat taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct
     1081 ttttaccttt ttatcggcta ttcttctata ttcgttacat caatattcaa ttcgaacaat
     1141 tgtaaaggcg aaaccatact tggtgcatca ctcatcacat ctgctgcttt tccgttttta
     1201 ggaaaggcaa tgacttcacg aatattatct tctccggcta ataacattgc aaaacggtcc
     1261 aatcctaatg caataccgcc atgtggaggg aatccgtaat ctaaagctgt taacaagaaa
     1321 ccaaattgtt cttccatttg ttcttttgta aagcctagag tttctagtac tttttcttga
     1381 agctcacgag tatgaatacg gatagaacct ccgcctaatt cataaccgtt caagacgata
     1441 tcataagctt ctgcatagac ttttgcaggg tcttcagcca ataacgggat atcttcttct
     1501 tttggcatcg tgaatggatg gtgagcagag acatatcgac cttcttcttc tgaaaattca
     1561 aattgtggcc aatcagttac ccataagaaa ttgaatttcg attcatcaat tagtcctaat
     1621 tcttttccta aacgtgtccg gatagcacct aatgcagcag cgacgatttc acttttatct
     1681 gcgccaaaca tcagcaagtc acctggtttt gcatctgttg ctttaatgat tgcttctgtt
     1741 gcttctccca tgaatttagc aattggacct ttcaatccgt cttcttctac tttcagccat
     1801 gcaagcccct tagctccgaa ttgtcctaca tattgaccta actgatccat gtcttttcgt
     1861 gagtagcgat ctgcagcgcc tttagcattc aatgctttca cgacaccgcc gttttcaaga
     1921 gccatttgga agactttgaa ttctacttct ttgactgtat cgcttagatc gatcaattcc
     1981 atatcaaaac gtgtatccgg tttgtcgctg ccgtagcgag ccattgcttc atcataggtc
     2041 atacgaggaa atggcaatgt gacttcgata ccgcgaactt ctttcattac cttcgcaatc
     2101 aatccctctg tgtaagtttg gatttcttct gctgttaaga acgttgtttc aatatctact
     2161 tgcgtaaatt caggctgacg gtctccacgt aaatcttcat cgcggaaaca acgaacgatt
     2221 tgataataac ggtcgaatcc agcattcatc agcaattgtt taaacaattg aggagattgc
     2281 ggcaacgcat agaaatgtcc agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct
     2341 tctggtgtag atttccctag gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa
     2401 taatgacgaa tcgcttttgt tgtcgcattt cttaatttga tgtttttagt catttttgga
     2461 cgacgcagat ccaaataacg atatttcaaa cgtatttcgt cactgattgc ttgatcatct
     2521 tcaatttgaa atggcggggt ttttgcttga ttcaaaatca cgatttcact tgcaatcact
     2581 tcgaattcac cagtggccat tttaggattt actgcatttt ctccgcgtct tctaacttcc
     2641 ccagtcactt cgatcacgta ttcactgcga catttatccg cgatttccca tgcttctttg
     2701 ttgatttcag gattaaagac gatttgaacg attccttcac gatcacgtag gtcgataaag
     2761 attactcccc caaggtctcg tcttttttgt acccatcctt ttaatgtaac tgtttgttct
     2821 aattgttgtt cggaaactaa tccgcaatag atcgttcttt gtgccattta tttttttcct
     2881 cctactcgaa taaagtcatt tcatcataga cttcatcaaa atgttcatat atctcttcta
     2941 atggaaaact tttttctttt cggcttgcca ttgctttgac attaatcgtt tgactagcaa
     3001 gctcatcttc accgatcacc aatactaatt tggcattcgc tttatctgct gtcttaaatt
     3061 gcgcttttgc tttacggttc atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg
     3121 cttggaccac tttcaatgct tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat
     3181 ccaactcatt aaatgcaggg ataactactt cctctgcctc catagtcaag agtaatcgct
     3241 cgatacccat tgcaaatccg aagccaggtg ttgaaggacc acctagttct tccactagat
     3301 tgtcataacg tccgcctgcg caaatcgttg cttgtgcccc catttttggt gcgtcactca
     3361 tgatttcaaa aatcgtatgt gtgtaataat ctagtccgcg aaccatatta ctgtcgattt
     3421 cataaggaac agccaatgca tccagcatgg aaacaacggt atcaaaatgt ttttgagcag
     3481 gctcgcttag ataatctaag atagatggag cttctgccac aaatttttga tctcgtttgt
     3541 ctttactgtc caatacgcgt aatggatttt catgaaggcg gcgtttagag tcttcactca
     3601 gctctgattc aaaaggtaaa agatagtcga tcaatgcttg acgataggct tgacgcgttt
     3661 ctttatcacc taatgaattg atgactagcc ggatttgcgt gatccccaac tgtttgaaga
     3721 aatcaagtgc catcaccatg ctctccacat cagttgcagg gttttctgat ccaaatgctt
     3781 ctactccgat ttgatggaat tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca
     3841 ttggtcccat ataaaaagtt ttgaacggtt tcgcatactc tgggccgaac aatttatgct
     3901 caacaaaaga acgaacgatt ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc
     3961 ctttgtcata aaaatcatac atctcttttg aaacgatatc cgttgtatcc cctacacttc
     4021 gggatatcac ttcatagtgt tcaaaaatcg gtgtacgcat ttcattatac tgataatctc
     4081 tgaataataa acgtgctgtt tcttcgacaa actgccattt ttctgattcg cctggcaaaa
     4141 tatcatttgt tccttttggt ctttggaaac tcatagtttt ctctcccttt cttagctatg
     4201 taaataaaaa aatcaccctt gttcatcatg aacaagggtg aatgaaattc atacacggta
     4261 ccacctaatt ttgaaagatt tctctttcct cttacgaata acgctcgtac gcgggacggc
     4321 ttttcactcg tccatctcca gagtgtcttt aggctaatga aactttagat cattttcagc
     4381 ctcgatgatc ctctcttgta aagtttcaaa aacttactcg ctctttcatt gacattcgat
     4441 attacttata acctactaaa aaccagtctc aaagtcaaga gatatctgac tatttcccct
     4501 cacaaaaacg ttttagttca gctttttggt attttgtttt agactagaca aaaaggagga
     4561 atcagctatg gcagaaataa gaatcggaca ttcacaagtc tacgcagaac agcttggatt
     4621 aggagcaaat gccgttggag gacataatct ttttgacggt ttggaagatg agacaggaaa
     4681 acaagtagtt cgtacagctt tgaatagcgg gatcaatttg atcgataccg cttatgctta
     4741 cggaaacggc cgttccgaag aactgattgg cgaggtactg aaggaaaaag aatatgatcg
     4801 ctcacgtgtc gtcatcgcta caaaagctgc tcatgtacca aataaagggc gaacatttga
     4861 taattctcct gagtttctca aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga
     4921 ttacatcgac attttttata tccactttcc ggatgaaagc acaccaaaaa atgaatcagt
     4981 tgctacattg cacgaattaa aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt
     5041 cacgttagaa caattgaaag aagcgaatgc agatggatat gtcgatgtag tagaagataa
     5101 atacagtttg atccatcggc aggctgaaaa agaattattc ccttatcttg aaaaaaacaa
     5161 aatcagtttt gttccctatt ttcctcttgc ttccggtttg ctgacaggaa aatatgaact
     5221 tggtgaggaa aaacaatttg gtgaaggaga tcctcgaaaa agaaatccag atttccaagg
     5281 agaacgtttc agagaaattt taaccgctgt cgatgtactt cgccccattg ctaaaagata
     5341 ccaagccact cctgcgcaac ttgttttagc ttggtacatg aagaatccac gagtgtctgt
     5401 agttatacct ggagctaagc gtccagagca agtatctgac aatgtccaag ctttagattt
     5461 gcatttatcc aatgaagatt atcaaaccat tgatgaagca tttcgtggat tttgatcaat
     5521 tgaccttatc aatttatagt aatatgaaaa aaggaagatc agcactgttt gatcttcctt
     5581 tttttcgata agtttatgct aatctaattc ttgtaaagta gcgctcggct attcggataa
     5641 gtcggaaact gattgaatgg aatcgtccaa tcttgttcag gtacagagaa ctcaaactta
     5701 tttactagct gatcggaaaa cacacgtaga ctatgaagag tgatccattc tcctgcacag
     5761 cgatgcattt gtcgaaaatt tcccccgcct tgtgcaatca tctcatattc ttctttatag
     5821 gaaatatctt ttgctttccc tacataccgt ttgatcataa aggaatctgg cgcttcaact
     5881 gttcgttcat catggtctgt tccatataaa tctaaaataa cccaactgcc ttctggtata
     5941 cgataaccat ctacttctac ttctttcagc gaaatcgcag gtagcattgg gaagaaagga
     6001 taatagcgtc tcatttcttg aataaacgga tcttgtaacg tcgaaaagtc attttttaat
     6061 tgctgataca aatctggtct actgaaaagt gcatgtccca tcaatgccgc ccacacagtt
     6121 agagcaaccg ttggacggat gatgttcaat aattcaacag ctgccacttc cagaggtaaa
     6181 agctgaccat ctaaatctgt ggcattggca aaagcatata atgccacatt ttcttttcca
     6241 gccacaggat tttgtcgagc ttcttcaatc aagccttgcg cccatttttc tgattttttg
     6301 cgattttcga caccttttat gtgatcaatc ggagaagtaa acgttccgct gatcatagag
     6361 atttgatatt ctgctaattt ttcgacctca tctttagaaa tcgctgataa attgatacct
     6421 gcccactcgc aaatagatgt aaagaaaacc atcttactta aatcaaatag ttcaaattga
     6481 ccatgctgtg cttcaagtgc ttgtgtcaga ttcttatcta agatacgatg ataatcttcc
     6541 attctttccg gtgtcattag atccataaaa atatttttcc gatggtgatg agcagctcca
     6601 tccaaagtct gtactccacc ttgaccaaaa agtgtcttca atactaattt tggcatagca
     6661 ccttttcgtt tgaaattcct tggatcataa aatacttttg ctgctgatga tccgtagatc
     6721 gtgatagctt ctttattgaa aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt
     6781 aactcctcca acatgttgta tcctttttga tacaattttt ttaaatctgt gattttgata
     6841 tctactactg gtacttcttt catcttcagc aacctcctat aaaaaataac ttgtataatt
     6901 taagcttaac tttttttccg actgctgaca aaagaagcgc tttttgcaga caaaaaagcc
     6961 acttaagtta gagtttatca gtctaactta agcgacgtgt ttcatagttt ttactagtga
     7021 aaaccagatt actttatttg tttttagaat caataatgat cgttacaggt ccatcattga
     7081 cgagagaaac cgacatatct gctccgaatt gtccggtagc gactgtgatg ccgcgacttc
     7141 tgattccttc attgaatgat tcgtataaag gaatagctgt ttctggtcgt gcggcttcca
     7201 caaagcttgg tcgatttcct tttttcgtat ccgcaaacag tgtgaattga gagatactta
     7261 aaatttgtcc tcccacatct tctacactaa tattcatttt tccttgttca tcttcaaaaa
     7321 tacgcatttg tgcaattttt tttaccagat agtccacatc tttttgcgta tcggtttcat
     7381 gaatgcctaa taaaacaagt aggccgtagc caatttttcc aatttcttgc tgatcgactg
     7441 acacagcagc tttacttacc cgttgaacaa ctgctctcat atctgtcctc ctatccattc
     7501 gtccggcgta cactgtagac atcaggtact gctttgattt tatccacgat actctttagg
     7561 tgggataaat tctggatacc aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc
     7621 cgtgcttcaa cgctgttgag atttttcgtt agcgcattga ctgtttgaag tacatcattc
     7681 aataagcctg aacgattata gccgtaaatt tccaaatctg catcatactc ttttcgtgta
     7741 ttcgatgtat cttcccattc gacttcaatc aaacgttctg ctacatttgg tttgtcaggc
     7801 tgaacattcg gacaatctcg acgatgaatg gatatccctc tgcctttagt gatataacca
     7861 acgatatcat caccaggaat cggattacag cagcgactga tacgaattag taagttttct
     7921 accccttgaa tgacaacgcc accttcatga cgtactttca ttttgtcagg ttcttttttc
     7981 ggctgattca tgatttcttc tgcttcttgc ttttgctgct cgattttttg ttctttacgt
     8041 tctttttcag tcagacgatt ggccatcgtc aaggggctaa cttctccata ccctacagct
     8101 gcatagagat catcttctgt ttgataatta aaacgatcga gtgcttcttg cagtttgttc
     8161 ttcgtcaaaa tatctttagg cgtaaatcct agatctgtaa tacatttgac cacggattcg
     8221 tggcctttga tcacattttc ttctcgatct tgggctttga agaaacgttt gatcttattt
     8281 cttgctttgc tagtagcaac taatttcaac cagtcgcgac ttgggccaaa tgaatttgga
     8341 gaagtcatga tctcaatgat atctccgttt ttcaatttgt aatcaagctg caccattttg
     8401 ccatttactt ttgcaccagt ggttttgtta ccgatatctg tatggatact gtatgcaaag
     8461 tccaatggtc cggatccttt tggtaactca gtaacgtctc cttttggtgt gaagacgtag
     8521 actttatcac taaagatatc tccttttacg ccttccataa attcagaagc atcatagctt
     8581 tcgtcttgaa gttcgagtat ctcatggaac cagcttaatt gtttcgtcat accatctggt
     8641 tctacttttt cattttttcc ttctttatag gcccagtgtg cagcaacccc gaattcagcg
     8701 atttcatgca tttcctgtgt tcggatttga atttctaccg gatttcctgc cggaccgatc
     8761 acggtagtat gcaaagattg gtacatatta gcttttggca tggcaatata atccttaaat
     8821 ctgccgggca ttggtttcca ttttgtgtgg attgctccta atacagcata acaatccttg
     8881 attgaatcta cgatcactcg aatagctagc aagtcataaa tttcgttgaa ctgctttttc
     8941 tgatctttca ttttacgata gattgaataa atgtgttttg gccgtccata gatttctgca
     9001 aaaatcccca gctcttctgt cgctattcga atatcttcaa cagtaccact tacgtatttt
     9061 tcacgttctt ctctcttcgt ctgcatcaag tggacgatac ggtaatactg ttttggattt
     9121 agataacgaa gtgctgtatc ttctaattcc catttgatcc ggctgatccc tagacgatgt
     9181 gcaagaggcg catagatttc taaagtttcc tgagcgatcc ttctttgctt atcttctcgc
     9241 aggtgcttca acgtacgcat gttatgtagt ctatcagcta atttgaccat gataactcgt
     9301 aaatcttgag ccattgctag cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg
     9361 gatttatatt ttattttccc taatttggtt acgccgtcca ctaacatagc aacgtcatcg
     9421 ccaaattctt ctctcagatc ttctaaagtg atttctgtat cttcaacaac atcatgtaag
     9481 aagcctgtag ccactgtatg gggatccatt tttaaatctg ctaatatacc tgcgacttgg
     9541 ataggatgaa taatgtaggg ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc
     9601 gcgtattcgc atgctttctc aacaaatgct acatgttcgg gacccatgta ttgactaacg
     9661 agctttatga caccgggtcc cgttaaaatt tcttcttttg tcacaggaat cgatccccct
     9721 ttcttttttc ggtaaaacgg gaatcgggac aaatatctgt ttgataattg tcccaaaatc
     9781 cgtgcagtta tacgtttttt tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt
     9841 tttcgtatcc attgtataaa taattgtttc tattatacaa attgttgact attttttcaa
     9901 ttctttctgt cgatactctt ttattaatga agaacgttag ctgaaagaag gattcattaa
     9961 ttcccattga tagctagcag cacttaataa ataaagaggt gctgtttctg tacgaaggat
    10021 tcttggtcct aatccacaaa gtatcccttt gtgttctaca aaataatcga cttcatcgcg
    10081 ggataatcca ccttctggtc caaagacagc taagattcga ctatctggtt ttgtttcact
    10141 aagaattttg gctgcatttg atttttcgcc ttgctttgct gattcctcat aagcaaccaa
    10201 gaccacgtca taatcatcta gccgctcaaa taaatcttgt ttcgtttcca ataactctac
    10261 tgatggcacg atctgccgat gggattgttc cgctgcctct tgagcaatct tttcaagccg
    10321 ttgttgtcgt ttcatacgtt tttttgcatc ccatttaacg acagaggctg atgccgggaa
    10381 accaagaaaa gcttgcgctc ctaattcagt ggatttctga acgatccaat ccagcttttc
    10441 ccctttagga tagccactcg ctatggtaat cgaacttggc agttcctttt gattttcttc
    10501 ttttttaatt tctttcagat aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat
    10561 ggctatttga tcattgaatg ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg
    10621 tacaatgtgg tgatagtttt ccccttctaa aacaaaatag tcttttgatt catatgattc
    10681 ttttaaaaaa taccgctgca tctattccac ctctggtttt ttcaaaataa ttgcatacca
    10741 gtctttttgc tggaaaattt gatccacgat aaacccttgt tctaacatgg catccagaac
    10801 catttgcttc ttctcttcaa tgattcctga aacgatcagc gtgcctgttt gcttcagtaa
    10861 acgccaagca tctgggatca tcaataagat gatatctgct aaaatgttcg caacgattac
    10921 atcactttca atctcgattc ctgtcaaaag atcgttagct gatacatgga catctttggc
    10981 tatttcattc atatccatat tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa
    11041 ggcatagaca tcttttgcac caagatattt tgctgcaata cttagcacgc ccgacccagt
    11101 tcccacatct agcaatgttt caccaccacg caagactgtt tctagtgctg tcaatgtcaa
    11161 ggaagtcgtg ggatgagtgc ctgtgccaaa tgccatacca ggatctagtg tgataatttt
    11221 ttctagagga tcttctgtct ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt
    11281 aatacgcacc ggatgatagt atttcttcca ggctgttgcc cagttgcttt cctctacttc
    11341 actaactgtt actttatttt ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg
    11401 atctttgata aaaggcaaga tttccggtaa gaaagtcgtt tccggaaaat aagccatgac
    11461 tagtgcgcct tctttcagcg atgtaaaatc atttttatct aaaatctcac catattgatc
    11521 cgactgaaaa tgttcaacat ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc
    11581 catcaaaata ttagcaactg cttcgactgc ttcactttca gtttctactt ttacttcatt
    11641 ccatttcata tctgtcactc cttaataact tgggtacggt aaataagtat ctgcttcatt
    11701 ttgctgtttt acttctacta attctgctag ttgctgatta tcccagtgaa gctcgaatac
    11761 agcttcgtca tcgtcttcat ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac
    11821 ttcatttaga tattccacta aagaatcagc tactgatttt gccaaccctt tttttccttc
    11881 ataagggatt actgctagat aattgcgttc atcaaaaaca gatttctgag gattaaaaag
    11941 aagtacgccg tcttcaaatt cgataattgg ttcttgcgaa acgatccctt ctgcatcatc
    12001 aatctcaaat tgtgctttat tttctgcaaa caagcgaaca acgatctcga tcgtatgatt
    12061 ttttgtatcc caatctaaag ctacatcata gtctgtcact tgtttttcaa ttatttcatc
    12121 taaatatgtt aatagatcct tctttttcat tttctttcct ccaaccccaa tctttctcga
    12181 tttggttttt tctttgtaaa gagatataag gatttcccga cacaacaaaa cgcaacggct
    12241 tttctgtcca tacacctttg tttgggatac cgattctagg caaagcattg atcttttttg
    12301 gtgtcttttt tttctcaaaa actaagcgta acgaactatc tgctatagac tgaccataaa
    12361 gttcttgagg aattgctaat gcttccacta attttcccgg accgttgctg atatctggac
    12421 cagtttttcc gccacgattt ttactcattt gatcaatcat tgcggcgggt tcgattgcac
    12481 gtatcattac tccttgagga atcccttctg gctgagtaat gatgttcaaa atccgatgag
    12541 tatgcatcgt gtaaaggtag atcgtacctg gtttttcata cattgcccgc acacgcggtg
    12601 ttcttcttaa accatagcta tgggcggcct cgtcttctgg acctaggtat gcttccgcgt
    12661 ctacaatata tccagctaat cttccttctg gtgtgtcatg ctccaaatac atccccaata
    12721 aatcccgtgc cacttctggc gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat
    12781 ccgtcatttt tctcacctac tttatcatac acaatattag ttggataagc tatgaaaaca
    12841 aaacaacaga aggctgttct ttctaggcat cttgctaaat atgatataat taagggacgt
    12901 aaaaacaaga aggaggaaaa tcctatgcaa cagccattgg cttatcgcat gagacctcga
    12961 acgatcgatg aagtcgttgg tcagcagcat ctagtcgggg aaggaaaaat catacgccgc
    13021 atggtcgatg ctcgcatgct ctcttccatg attctatatg gacctcctgg tactggaaaa
    13081 acgagtatcg caagtgcgat cgccggctct actaattatg catttcgcat gctaaacgcc
    13141 gctacagatt caaaaaagga tctacaagtt gtagcggaag aagcaaaaat gagtggtaca
    13201 gtgatcctct tattggatga agtacaccga ttagataaaa caaaacagga ttttctgctt
    13261 cctcatttag aaagtggccg aatcatcttg atcggagcga ctactgagaa tccttatatt
    13321 accatcaatc cagcgatccg cagccgtaca cagatctttg aagtcaaacc attaacagaa
    13381 caagacatcc agttggcagt cgaacatgct ttaagggata aagaacgcgg ccttggacaa
    13441 caagcaatcc agttagacga agatgctctt cttcaccttt cgcgtgcaac gaacggcgat
    13501 ttgcggagtg cattgaatgg gctggaactg gctacactat cgactccttc agataaagaa
    13561 ggaaggattc atcttacact gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc
    13621 catgacaaaa atggagatgc tcattatgac gttatttcgg cttttcaaaa atcgattcgt
    13681 ggaagtgatg tggatgctgc acttcattat ctcgcccgtt tagttgaggc aggtgatctg
    13741 gcaagtattt gccggcgtct gatggttatc ggttatgaag atataggttt aggtaatcct
    13801 gctgctgcgg ctcgtacagt gaacgctgta ttagctgctg aaaaattggg attgcctgag
    13861 gcacgtatcc cactagcaga cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct
    13921 gcctacatgg cattagatgc agcacttgct gatattcgag agggcaaagc tggagatgtt
    13981 cctgatcatt tgagagatag ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt
    14041 tatcagtatc ctcatcattt tgatcaggct tgggtcaatc agcaatattt accagataaa
    14101 ctgaagaatg ctcagtatta tcagccaaaa gatacaggga aatatgaaca ggctctgggt
    14161 caacagtatt accgtataaa agagtggaaa aagcatcctc caaaaaacta gacaagaaaa
    14221 cggttctgtt tctgcgagct ctctcattgc tctttttaaa ttcatatgct actatagagg
    14281 tgggaaatct gtgatgttcg aattgtcctc tttgtgtgtt gaccgaacat ttttattgat
    14341 atcttgggac ccgtcttgtg tcactgctgg taacatgcct tatcatttgg tagttcgaag
    14401 ctacgaccat gggacccacc tgcttgaatt cgcgggatca atactacagg acaaacaacg
    14461 gcattaacgg agattaccat cttttaaaga ataaagcaat ttgctcttgg caaattgctt
    14521 tttctatttg ttgcttattt tggaggattt gttcatgatt aggattttaa tgattatcgc
    14581 cactttagta ttattattcg ttagctatta tttatttaat aaacaggata tcttttttgt
    14641 attgatcaac aaaaatgata aaaatcaagg attcctacaa ttttacggag ctgcatatgc
    14701 tgttttaggc gtaatgggta ttttaacagc ttttttcaat caacgcttta ttgccttggt
    14761 ctttttactg atcgtaatta ttgtttctgc caccttcagt atccgatttg caaaaaaaat
    14821 tgctgaacca aagcagtaaa taattctctt tttccctaaa gtgttctaca ataaaagagt
    14881 acaatacgat tgacttgagg agtgaatgct tatgttacaa caatacaaaa aaatcatggt
    14941 tgccgtagat ggttctgatg aagctgaatt agcttttaaa aaggcggtca atgtagctat
    15001 ccgaaataac ggagaacttc tattagcaca tgtcattgac acacgttctt tccaaaccgt
    15061 ttcatccttt gatggaatgc ttgcagaaca agctactgaa atggcaaagc aaacattggc
    15121 agattatgaa agcaatgcga agaaagctgg cttgaataat gtaacctctg tcgtagaata
    15181 tggctcacca aaacaaatta ttgccagaga aatcccagaa gataatcaag tagatttgat
    15241 tatgttagga gccacaggat tgaatgctgt agaacgttta tttattggtt ctgtttctga
    15301 atatgttatt agaaacgcag cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca
    15361 attgccagct aaagaccaca ccaaagctta acacgtagac gacctgccaa ctatcggcag
    15421 gtttttttgc acaaaaaacc gtgataaaaa caaaagacgc ggcaagcact catcggtctt
    15481 tataatgttt aaaataaagg gaaaacagtc aatgagttca agtaaaataa aaaaaagtga
    15541 acaccagggc gattagtcgc cctggtgtgg tttaaaacga cctaagcatg ccaaagctta
    15601 ttggttcgtc aatgtggaag tttccgatga atctgatacg aaaaaatatg aagtatcgat
    15661 ggcggatacc gattttatcg ccaaactaaa attttatttc gggttcgggt accgcgagaa
    15721 ggcaagtgct acattcggta aatcattcaa gaattgatga aaggctaccg cttgccattg
    15781 caaccacaac gatactcctg aacgcgatgt tggtgagatt tccaaagaga agagctgttt
    15841 aatgcaacgg tattaagcaa atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa
    15901 caccatatga tttcgcctga acgctggttt agctcagaat acaatgatgt ggtccacgag
    15961 acattaccct cagtcagata aaaaaagctc tctgacagaa ttacacatcg ataaatatta
    16021 agtaaatgtc agcgctagtt gaactttctg acactttcag gtacactgga aaagacaact
    16081 acacgaagga gggaaaatac tatgatgcgc atgactaaaa taacaagtaa tgaaaattac
    16141 caacgatacc atatcgcatt tagccatgca attggtttta cagttgctgt tgacgggaca
    16201 agtttgtcca aaacagttga attcacgtaa aatcaatggc catctcaagg tatttttctc
    16261 atgataatct gagtttaaat aacacaggcc atggataacc atggcctttt tgagtggtct
    16321 caaggagaga gatcatgatc atacaagcaa tttctattaa aaaaacaatc ggcgggaatc
    16381 cattatttga ggatttatcc tgtcaaattg aagctggtga aaaaattgcc ttagtcggta
    16441 tcaacggaac tggcaaatca acctttttac aaatcttgac cggcagagaa ggtgtcgaca
    16501 gcggtgttat cagtcgcaaa aaaggcttaa agatcgggac ggtcgaacaa gaacttacag
    16561 tttccgaagc gaccgtcaac cactacttgc tccattctgc cgctgagata caggacctca
    16621 aacagcaaat gagtcgctac gaagcattga tgacagaacc agatacaaac ttagagaagt
    16681 gtctggcccg ttacggtgag ctgcagcatc gctttgaaga actaggcggt tacgtattag
    16741 aagaccggat tgcagcgatc ttgcaaggct tagggattcc acaccgtcag gaagcacggt
    16801 taacagaact tagcggaggc gaacgagtta aagtcgcttt agccaaaatt ttagcagccg
    16861 atgccgattt acttttactc gatgaaccga ccaatcattt agatcttaac agtattcgct
    16921 ggttggaaaa ctatttacag aatacaaaaa aatcggttct ggtcgtttct catgatcggc
    16981 aatttctcga tcaagtcacc acaaaaacat gggaactgga agaaggtgca ttgattgagt
    17041 atcccggaaa ctacagccgt tttcgagtct tgaaagaagc tcgcttggct gagctgacaa
    17101 aaaattacga actgcagcaa aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc
    17161 gtcagtgggc acatgaagga gacaatgagt ccttcttcaa aaaagccaaa gaactggaac
    17221 gccgcctggc gaaactgaca ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa
    17281 gcttgtctaa tggtggaaaa tctggcaaag aagttttcat cattcaaaac ttgcaccagc
    17341 agtacgctga tcaagtacta ttcaaagaca gcagttttgc agtctatcgt ggagatcatt
    17401 tagccatcat cggcgacaat ggtgcaggaa aatcaacctt actcaaatta ttactcggtg
    17461 aggaaaagcc ttacggtggc accatcaaat taggtagtag cttgcaaatc ggttatttgc
    17521 cgcagcagct gcagtttgcc gatcccgata gccgtttgct ggcttacgcc atcacattga
    17581 ctggaaacga agaagtcgct cgacggcaat tagccaaaag tggcttttat caaagcgatg
    17641 tgggtaaacg gatcaaagat ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac
    17701 tctttttaga aaaaatcaac gtcctgattt tggatgaacc aaccaatcac ctcgacagct
    17761 atgctcgaga agaaattgaa gaaatgctgc aagactatca aggaacgctc ctagcagtca
    17821 gccatgaccg ctactttttg cagcaacact tccaagaagc actcgttatt gatcaggagc
    17881 agatcagccg ccagccgctc ggccaactga ttgactagga cgttttttcg caacaaaaaa
    17941 cttgattttc atacctgtgc tttcacgatt caggcaaagt acaggtggaa aatcaagttt
    18001 ttattattcg atcaatgaat gtttcggatc ggttttctag ctaaacataa agcgaagaat
    18061 aaagtcgaca gcccacgagg ctgatctaaa aatgtttttt ctttttacat atgtaatgtc
    18121 ccttataaag atgaatgaaa ataaaatatt gggattttcc tgaaaatcaa taaataaaat
    18181 gccagtcttt tcccttttga ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa
    18241 aaagaaggga aattgaagtt aaattaccat tttgcggtct tctccgcaaa aaaaacacat
    18301 gcaagaattc cctcttgcat gcgtttcatc ttttatccta acgaatgctt tggcttagcc
    18361 gcgcagacgc tccacatcac gggcaatcat tagttcctca tctgttggga tcactaatac
    18421 tcttactttt gattcttttg tagagatatc tacttcttca ccacgcaatt cattcaacgc
    18481 tggatcgatt tcacaaccaa accaagacat acctttgatg atttcactac gtacatgtcc
    18541 gtcattctcg ccgataccag cagtaaagac aattgcatca acaccattca tcgttgtcac
    18601 ataaccgccg atgtattttc ggatgcggtc cacgaaaatg tcataagcta aacgaatatg
    18661 ttctttttca tagttctttt caagatcacg catatcacta gacaatcctg ttaggcctag
    18721 cagaccagat ttcttgttca aaacattgat catttcatca atatcaatct ctaatttttc
    18781 cattaaatat gggagaacag ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc
    18841 agctaatggt gtgaatccca tagaagtatc catggattta cctccatcga ctgctgtgat
    18901 cgatacacca tttcctaagt ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa
    18961 taattttgcc gcacgttccg acacataacg atgacttgtg ccatggaagc catattttct
    19021 tactttgaaa tcttcataat aagacaccgg taagctatat aaataatttt gctttggcat
    19081 tgttgagtgg aaagatgtat cgaatacagc cacactcaaa atgtcaggta aaatcttttt
    19141 aaacgctttg atccccatta agtttgcagg attatgcaaa ggagcaaatt ctgctaattc
    19201 agcaatgcgg tccattactt tctcatcgat cactactgaa tccccataag tttctcctcc
    19261 gtgaacaaca cggtgcccta ctccggtgat ttcatcataa gagcttaaaa tttgcagttc
    19321 aatcaactga tctaacagca tttttactgc tacttcgtgg ttttcgatat ccatgatttg
    19381 ttcgaatttt tgattatcgc cgtacttgat ggtaaaaata gaatcttgta aaccgatacg
    19441 ttcgacgatc cctttcgcaa ctacggtttc ttctggcatt tgatataatt gccatttcaa
    19501 acttgaactc ccagcattga ttgcaattgt tttagacata tgactctcct tctcgttgaa
    19561 tgattacaga gcagatgatt tccagttttt aaactcgttg aaaaattccg tcaccttctc
    19621 tggttctttt aatgaagcaa gtttcacaag caagacttct tttacttgtt gtgcttgtgg
    19681 tccttttttc tgtaagatca aaatactttt ttgcgattgt ttattacgga acaattcatc
    19741 tggcaattga atcattcctt gaagataacc ttcttcttta aaccattttt tgatttcttc
    19801 actttgatcc gtttctaaaa atccactcgg catcaaaaac aaaccaaaac catccggctt
    19861 cacatatttc attgattgct ctagcaataa atgatgtgca taactatggc cttcttcggt
    19921 acttgttaaa aactcttttg ctttttcgtc attcggataa taaccgattg gcaaatcgct
    19981 gattgcaaaa tcgacaggat ctattaataa ttcctgcaac ccgtcttgat gaaagtattg
    20041 aacatttgct tgtgtcaaat cacttgtaga agctgcgaca gccaacaatg tatcatcaat
    20101 atccacacca attccttctg tttggtaccc cgcattcgat aggtttaaaa gaactgttaa
    20161 taacaagttg cccattcctg ccgcaatatc cagaatctta accggtgctt ttttatttgt
    20221 gtatagttgt tcaataagaa aaacaaataa aaaaccgata ctatcaggag tgagttgatg
    20281 gttcgcctgc aaatgttctg tctggctgcc cttcaataaa agtaattgag acagtctgcg
    20341 ccattcttct ggctcgaaag aaagtttctt caactcttca taaagagctg tgatccgttg
    20401 cgttgtctct tttgtcggca ctccatccac cacacgtact tgataatcat ctattaaatt
    20461 ttctgcattt tcgacatagg cttctaaaaa ggaagttccc aatgcattct gtaaaagctg
    20521 aatagcttcc aggttttgat taaacccttg ttccattttt tctggtaaca tggtgcacct
    20581 catttctaga tatattataa caattttttc acctgtttaa ttctatcgaa caattgacaa
    20641 aatttcaatc gttttcactg ttttttattt gtgaaacggc aaacattttt ttattaaaaa
    20701 cagtgttata acagaaaaga gggcgttttc acgaacattc catcattctg tactagtatc
    20761 tgtatcattt tttacttctt ctgtgaaccg aaagataaac ggtgagactt ccacctctaa
    20821 gaatagggac tgttgtaccc actgatatgt tacagatcca gttgtgtagt aaaaaactcc
    20881 ttccgaactt tcggcaagcg tagactgttc cgataaagcc atctgcttca ttatttttgc
    20941 tatataataa tttttgattc gtatattaag atccattgac aaacggtagc tttctaatgc
    21001 tgccatcaat aaaaacgtac ataaaagaaa taaaagcaag acagaaaata aaatagagcc
    21061 cttataacat tttctactga tcgttttttg tccatatggc atatccatct tctccatttt
    21121 caaagtgtac ataaaaagaa attgtgtgtt ctttttccaa taattgaagc tcagtgatac
    21181 ttgttaacat aggttgatgt ccgccggaag ttcttctttt acgtatcatt gcctgatatc
    21241 tttcgatgat atactgttta tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga
    21301 cattgtgact gctcttgagt tcttcttcta attgtgctaa aaatattagc cactcttttt
    21361 gatctttacg ctgcaaaaat tcacttactt ggatggtttg ctgaatcaaa agattgaatg
    21421 tgagcaatag actgctcaat atcatcagtc caattagaca ttcaatcagc gtaaatgctg
    21481 gaattacttg ttttttcaat actgatcgct tctttctcca tattgacaaa cactttttcg
    21541 attactccat tattatcgac aatttggata tcgtatggga tcatttcttc ccttcgctct
    21601 ggtcttttgt gatacatccg ctgcgtcttg atatcttcat aaatcattcg aataaatagc
    21661 agttcctccc cacttttctt ttctgcctga agcaaaaaca gctggccaaa gctcactaag
    21721 tagacacaga cagaagcgat cataagcgct gtaagggact ccaacaaaac aaatcctgtt
    21781 ttattcaatt ctttttgtat agcgaccact ccctatctgg aattgatacg taatcgtccg
    21841 ctctttttct tcccaataaa attgataagc ttttagtgaa ctttcattcc cggttctagc
    21901 agcaaaagta atagaagcat ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc
    21961 ccaatttatt ttatctggat tcggaacatg aaaaatgatt tgattctctt cactattcca
    22021 ataaaagcct gtttggatct gattcacaat agcgattttt tgcgtagaat agattcgttt
    22081 ttcaaattgg ccgaaaaatt gatagactgc caattcattc ttccatgcag aaaatgcgag
    22141 aactggaaaa gaaaggatcg ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt
    22201 atatcctgat gctaaaacct taccttcaag gccgctgata gatttctgca tgttctcgtt
    22261 tgatatagcc ttcattcaac aattcctcca cagtaggaac tcttccatta ttttgaagcg
    22321 catagagctc tttttgtgtg tctaccaact ggatgatcgc ttcacgactt tcttggtcta
    22381 catgattcct ataacgcgac aaattaggga cgaacaacaa cactaataca ctgatgatca
    22441 gtaagacgat tagcatttcg atcaatgtaa aaccgctata ataagatatt tttctctttt
    22501 tcatggcaaa acctcctcta tgcttccgta aacaggtaat aaaacagcgg cataaactaa
    22561 taaaatcaaa gccgctacgc ccaaaaaaat gattggctga ataaaatgca tccagtgatg
    22621 aagacgtttg aaaagctgtt ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa
    22681 acttcccttt gcctctccct gtaaaacaat tttactgaat tcttctgata gaaaacgata
    22741 attctggaac tgctcagcca atgacatacc tgcctccaat ccttgaatca acttagaggc
    22801 aagatgctga atcagacttt cttgtttcgt ttcctttaag cagactatga tttgccttaa
    22861 ttcgaaacct tcatgaaaaa gctttccaat ctctagtgac aaataagccg attgatacaa
    22921 ggaatatatc ggtccaatca ataattgtct gcttatccat tcactctgat caacaggaga
    22981 caacttcatc agacgatacc ttactagtaa agccaataga gtaaccgcta ggatgaatag
    23041 tagaacatac caatgaaaga cttgtatcag ctgaataccc caatgctctt gcatgaccat
    23101 tcctgaacca ataagctgag gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa
    23161 aaaggtgagc aatagcatcg gatatgcaag cagctttttc aaatcattgc gaaatccttc
    23221 aacaagccga aactgtttgg caatttccaa caaagttgtc ggcagattcc catgacgttc
    23281 agctaactcg atctgtacca gttgctctgc tccatatcct atttgcgaaa gtacctcact
    23341 aaacgcttgc ccttccttta gctgatgttg gatatttgcc aaaataacag gtggaaacga
    23401 acggatcgtg agtaataaat aaagggattc ttgcagacta aaaccagata aaagcagttc
    23461 tcccatggtt tgtgcaaact ggttttgctg gcttcttgtt aatcctttag aatagttctg
    23521 actgccaaat attttcctga gcttcttcat aactttcctc ccatgatttt tttactgatt
    23581 cttcctccat aaattgataa ctccataaaa cagaaggatg gtgattggca tcttccaaaa
    23641 gttcttgata aatgacacca gctaaacatt cagatagctc ttcttttttc tttgtcagtt
    23701 catggattct gaccaatgta ccttcaagat tgcgtgcatg tacggttgca aaaacccggt
    23761 gccctgttag tgctgctctt accgcagcat gtgcagttga ttcatctctt atctcgccaa
    23821 tgattagcaa gtctggacga tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg
    23881 ctattttcgg attgatctgt agctgcagaa aaaacggttc ttctatctct acaggatctt
    23941 cgatagtaat cacttgcaat ttttcttcca aagccaaccg atacattaga gaagtcttgc
    24001 ctgatccgac tggtccgctg aaaaggtata atccacgtct gacggttttc tgtaaaatag
    24061 ttgcaaaatc tcgagaacta aagcaacgat ggggtgtttc aaaagtaccg tataatagcc
    24121 gaatcactaa actctctcgt tgaaggtaat ctcctacaga agacaaccgc agacgaactt
    24181 cctgtttttc taaagaataa gtaaatgcac ctaactgagc ttttcttttt tctccgatat
    24241 ccatctgtcc caaatactta aatcttgaga tcagttgctg tccatctgcc gaagagactt
    24301 tcttccagaa tgttcgtttt tttccatagc gaaaatagat gtaaaatact tctgaagctg
    24361 gcaatatata tacgtcttgg gcatgtttgg caaaactttc acttatcatt tgatccgata
    24421 atcgttttat ttccattatt ctcccctcca caaaaaacat acgtcttttt gatctttttg
    24481 tttcaaataa aatgaattta caaaaaaata ttggcaaaaa ccgaatttat gttatactta
    24541 taaaaattta tcttgggagg cactccattg gaaacaacat taaggaaata tatcgaagta
    24601 gcaagtaaaa aaaagcctgc tgacctagtg atcaagcatg ctaagatcgt caacgtcttt
    24661 acgaaagaaa tcatggaaaa agatgtagca atctgcgaag gaatgatcgt aggaatcggt
    24721 gaatacgaag gaaaaagcgt acataatgct aatgggcaat atctagtgcc aggattcatc
    24781 gatggccatg tgcatatcga aagctcactt ttatcaccaa gcgaattttc aaaagtatcc
    24841 ttgcttcatg gagttactac tgtagtgaca gatccccatg aaataggaaa cattgctggg
    24901 agtacaggtc ttgagtttat gatagaagat gcaagacaaa cactcatgaa tatctttttt
    24961 atgctacctt cttgtgtacc tgtaacccct tttgagacca atggtgcaaa tttaaatgca
    25021 aaaaaattag cccctttttt acaaaaaccc gaagttttag gcttagctga agtcatgaac
    25081 tatcaagcag tagcaaataa tgaaacagac atcctagaaa agatccaatt gatgcaccag
    25141 cataagaaaa aaattgacgg gcatgcagct ggtatcggca tggaagagtt aaatgtttat
    25201 cctgcagcag gtattcggac ggatcatgaa gctacaactg caaaagaagc taaagaacgt
    25261 ttggatcttg gtatgtattt aatggttcga gaagggaccg ttgcaaaaga tctggcatcg
    25321 ctactaccgg cgatcacacc tgaaaattca agaagatgct tctttgtcac cgatgataaa
    25381 ctgatcaatg atttagtaaa agaaggatca atcgatcata tcgtccgtca agctattgca
    25441 cttggaatcg atccattaca agccattcag atggcaacat tgaatgcagc agaatgtttt
    25501 gatttgaaaa cactaggtgc gattgcccct gggtatcaag ctgatttctt cttaacagac
    25561 gatcttacca agctgccaat tcatgaagtt ttctataagg gaacattagt tgttcaatct
    25621 gggcaactcc aagaccattt atttgctgat cattcaaatt catatacgtg ttgtctgcca
    25681 aaactaaacg ctcaaccatt aaatccacat tcgttagagc tgcttctttc ttcttcacaa
    25741 gcccatgtga tcgaaattat cccgaatagt ctagtgacta aagatttggt agaaaaagtg
    25801 gaccgacaag gcgaacgatt cattccttca atcgaaaaag accaattaaa gattgcggtg
    25861 atcgagcgtc accatcaaac gggaaacatt ggattaggca tagtgaaagg atttcagctg
    25921 aaacaaggag cgatagcgac aacgattgcc catgactcgc ataatctagt tgtcgttggg
    25981 acaaacgacg aggatatgct gtatgctgct aatcttttga ttcaaaaagg tggcgggatg
    26041 accgttgtca acaaacagca ggaaatagct tgtgtttctc ttcctatagg cggaatcatg
    26101 acacaggaac ctttttcgat cgtctatgaa cagttgaatg aattaataac taaagcacat
    26161 caactaggag cagctaaaac ctttgatcct tttttgacac tttctttctt aacattatct
    26221 gtcatacccg aattgaagat caccgacaaa ggattatttt cattttctcg cttccaattg
    26281 attccacctt gtggaaaaac cgatgaaata aatacatttt aaatcaaata aatcccagaa
    26341 aatacattac atctgtaatg ttcattttct gggatttatt tatggatatt aatgttttta
    26401 tgaaagcaga aagttagttt cacagacttt tctttttggt cttatcttga aattgagctt
    26461 ctactcgata aatacgttga ccttttgaag aaaatttttc ttcatactcc gtcataatat
    26521 tgccttcaaa ttgactttga tgtaaatcta accatacctg tttgaggatc atgccatatt
    26581 gagaaaaact tgccagagaa tactcaaaca atccttgatt atctgttttg aaatggattt
    26641 ctccatttgg acgaagaatc tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc
    26701 gtttttcatg acgttttttt ggccatggat cggaaaaatt cagatagatt tgatctactt
    26761 cgctgtcagc gaaatactct gtcaacgctg atccatccac atgtagaagt ttcaggttag
    26821 gaagcggttg ttcgatcaac ttgtctagtg caatcgaaac aacactgacc tgcatttcta
    26881 ctccaatata attgatttca ggatgggctt ttgccattcc agtgatgaac tggcctttcc
    26941 ccataccaat ctcaatgtgg attggatgat cgttttcaaa caattcattc cattttccct
    27001 tccacaatgt tggatcacta attacaaaat tcgggtgagc cgctaacatt tccgctgctc
    27061 ccggacgatt tcggacacgc attatttatc tccattcctt tcctctaatt aaaaaggtct
    27121 ggccaaatct ccgcagagtt tgggccaaac ctcagccact gaaaattcgt tttaattgta
    27181 aaatttcttc gttcatccgt cgatgatctt cacgtttgca atatctggtg atttcttgga
    27241 gcaaattcat ggccgcatac caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc
    27301 cataactgat cagccactga ttccagctag acagtggcac ataatttcct aaaatcgttg
    27361 caatatccac cgcagggtct gcaaacatga ttgaatccca atcgactaga tataaataat
    27421 ttttacatac taaccaatta cgatggttta catctccgtg tactactgat aagttttcct
    27481 tatctattga cgggatctgc tcacgaagaa aacgatggac aatttgcaga aattgatttt
    27541 tctggagttc ttccggtaat tctttttcat atttttgcag catcttctcc ggcgtcatga
    27601 cacgaccacc gatacgggtc agcatggttt taagagaatt tgaatgatgt aaatggtaca
    27661 aaacatcaat cacgtcgttt ctttgaccaa tctcattagg agtcagcaag cgaccttcca
    27721 gccactcttg tgctgtcaaa gtatcgccat tccccgttcg ttttgtccaa acaagttttg
    27781 gtgtaattcc ttcccttgaa agagcagcca acatcggagt cgtgtttcgt ttaataaaga
    27841 ctttgtcctc atcacgcatg cctatgtatg tttttcctgt gtcgcctttg atgggacgta
    27901 accgccactc ttggtctaac tgaaactcca tcatgcattc tcccgtcctc ttactctttg
    27961 ttttgcagta cttattattc tagtcgcctg aacactacac gtcaagcaca tatacgttct
    28021 ttttcattta attgccattc aaaaaaccag cttttctcaa aaaaaataga acgaacagca
    28081 ttcttactgt caacgctcta tttttcattc tcatttttac cctacacagc tttgttttca
    28141 gtctgacatc ttcttcaaac gagttggtac atatagttta acaaagagca tagtaacaat
    28201 caaataagcc gctagcggaa cccatcgctg ctgcccatct aatacgactg cagcaatcaa
    28261 accaaaaata accgacgcga taattaatac ccatgtgagt agatactgaa tcgcctgctt
    28321 cttctgattc ttatggattg gataaagctg aacgagaacc atatagcgga actggttcgc
    28381 taaaggtaac agttggaaac cgatcaaata taaaaataag aaaccgataa tcagactaaa
    28441 ataaaagtcg actactccgg caatcaataa cccacctata attactaatc ggaaataaag
    28501 accactaaaa tcagaacctc ttgccaatct tcttgcaaac aagtaaagat aagtgttttt
    28561 tgattctctt ttaatatgag tcaatatagg atccaaatag cgtcttcttt tgatctttgc
    28621 cgtaatttca ggaacatctg tgaataaatt gataaattga tagatctgat gcaacctatg
    28681 ttcttctaca tgcaccattt tctcccaatc caatcctttt tcttgttgct tccacaaaaa
    28741 ccagtaatat agacttgcct gtactaatgc acctgctatt cctgcccata ttagccaata
    28801 aaatgataga aacaaaaaga tcagagaaga aaaaaaccag cctaatttcc aataacgaat
    28861 aacttgttga tctagtcgaa acaattctgc tctcttgatt tgcagatgac tgaatttcaa
    28921 actccataat atgatcagat agacgaaaaa catcgaaaag gattgctccg tactaaccac
    28981 cactaatggc atggccaatc cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga
    29041 atagcgaaaa gctcgttcca aataggtttg catttctttt tctttaggaa gtaaaaaaac
    29101 aatatctgct gcctctgcaa aagtagccat cttacccacg aataaacaga gcatccaaaa
    29161 tacagagatt atgagccctc caattgcaaa cggtggctgc agggttttca accaattaga
    29221 ataatataat cccacaccgc caatcaagaa aagacaaact aaaacaaaat gatcgttcaa
    29281 tacataacgc atatatttca tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa
    29341 actactcatg tgcctgctcc tttgttaaag caatatagat ttcgtccaaa gaagaatccg
    29401 gcagctgaaa ttcttttctc aacatctcaa gagaaccgtc tgctcgtatc tctccgttat
    29461 gtaagaccac aaaacgatca caatattttt ctgcagtagc taaaatatgc gtagacatca
    29521 aaataccggc tccttctttt ttcatttcta ccatcaattc aagcaatgca ttgatagcta
    29581 gtggatccaa gcctaaaaat ggttcgtcga tgatatataa agaaggctcg atcaaaaagg
    29641 cgcataagat catcactttc tgtttcattc cctttgaaaa attagctggg aaccagtcaa
    29701 gacgattaga taaccgaaaa gtctccaaca acttttctgc tcttttcatt gctatatcca
    29761 taggtatatc gtaggccatc gcagtgactt cgatatgttc tctcaatgtc aattcttcat
    29821 ataaagaagg agtctctgga atatacccaa tttgtttgcg atattcttcc gtatttttct
    29881 ttaaggatag atgattgatt tctatcgttc cttttgaagc ttccagcaga ccgataatgt
    29941 gcttgatggt cgtacttttc cctgctccgt tcagtccaat caaccctact atctctccag
    30001 gctgtacatc gaaactgata tcttttaaca cagggagatg tccgtacccc cctgtgacat
    30061 gatcgataat taaactcatt ctttttcctc cgccatttat ttttatttat tataccacga
    30121 agaaaatgac ggtattttgt ttttttcctt acaatagctt taacttatgg taaagttaag
    30181 ctgaagtatt tattctttat agaaaggatg ttttatatgg aaaactgtat tttttgcaaa
    30241 atcatcaatc aagaaatccc aagctataaa atatatgaag acgacaaggt ctatgctttt
    30301 ttagacatct ctcaagcaac gaaaggacac acattagttg tgcctaaacg tcatgtagca
    30361 gatatttttg aatatgatcc cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt
    30421 gctcaagcat tggaaaaagc ttttccagaa atgaaaggtc ttaatatctt gaataacaac
    30481 cgtgaactag cctatcaatc tgtttttcat tcacatatcc atttagtccc tcgctacagc
    30541 aaagaggatg atttttctat tcactttgtc aatcatcaag atagttatgg atcggaagaa
    30601 ctaaaagcta ttcaagaaac aatcgtaaag caggtgtcct gcgatgatta aaaacttttt
    30661 caaaggactg ttatttggag ctgctgctgg cactgtcgga ggtctgttat tggcgcccag
    30721 gagcgggaat gaaacaagaa agaaactgat cgaagaattg gaagaagcaa caactctgac
    30781 aaatgattta aataacagcc tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac
    30841 agctgaatct gtgattcctg ctttccaaga agcaatcaaa aaagatatcg aagattttaa
    30901 attccaagcc gaaccacgta tcacacaaat caatgaacaa gtagaaaaac taaaagctga
    30961 tctgccagag tatcctgaac aaatataata atgattattt tctcaacaga tttaactata
    31021 taagtgagaa aagtttgcgt atttcctagg aatgtgatga aattttgaat tttgtttgaa
    31081 actaccacga caacgttttt aaaggaaatg ggatatggta tagttaatat ataaaaataa
    31141 taactaatta caggagtgca aattttaatg aaaaaaaaat caattatttt ggcagctact
    31201 agtgcgttgg ctgttttaac tctagcagct tgttcaggcg atactaataa agatatcgca
    31261 acgatgaaag gcggaacgat cactgtatct gatttctatg atgaagcaaa acttgaatca
    31321 tcgaaccaat ccttagtaca acggatgatc atttataaag tattcaataa caaatatggc
    31381 gataaagtaa ccgataagca agtggatgca gaatacgaca aacaagcgaa atcattaggt
    31441 gatacttttg aatcacaatt agaagctgct ggatatacga aagatactta taaagaatat
    31501 attcgtaaca atttagcttt tgaagctggc ttgaaagctc acgtagatat tacagatgac
    31561 gatttgaaaa ctgcttggaa atctttccat ccagaagtag aagcacaaat catcaaatta
    31621 tcaagtgaag atgaagcaaa agatgtgaaa aaatcagcgg atgatggaga tgatttctct
    31681 aaattagcca aagacaaatc aactgataca gagacaaaag aagatggcgg taaagttaaa
    31741 ttcgactcaa cgacaacaac tattcctgca gaagtaaaag aagcagcttt caaattgaaa
    31801 gacggtgaga tctccgatgt tattactaca acaaatccta cttcttatgc gacagaatac
    31861 tacgttgtca aaatggtgaa aaaccaaaat aaaggcaatg acatggataa atacaaagat
    31921 caactaaaag atatcgctac tgaaactaaa ctaagtgaca atgcattcac aactaaagta
    31981 atcggtgaag aattaaaaga tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg
    32041 ttgagtgcat tcacaacaac ttcatcaagt acgaaagact caagtgaaac tacagcttca
    32101 acaaagagtt ctgacacaaa atcaactgat tcaacaaaag aatcttcaac tgaagaaaca
    32161 actgattcat caaaataaaa gaaaacaaaa aaagcgaaga cttatgtctc gctttttttg
//
LOCUS       DADQRX010000022        32008 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.022, whole genome shotgun sequence.
ACCESSION   DADQRX010000022 DADQRX010000000
VERSION     DADQRX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.022"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            370..690
                     /locus_tag="J8V14_000982"
     CDS             370..690
                     /locus_tag="J8V14_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBA0921102.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            844..1749
                     /locus_tag="J8V14_000983"
     CDS             844..1749
                     /locus_tag="J8V14_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0921103.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1746..2519
                     /locus_tag="J8V14_000984"
     CDS             1746..2519
                     /locus_tag="J8V14_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0921104.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2700..4124)
                     /locus_tag="J8V14_000985"
     CDS             complement(2700..4124)
                     /locus_tag="J8V14_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0921105.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4335..4781
                     /gene="nrdI"
                     /locus_tag="J8V14_000986"
     CDS             4335..4781
                     /gene="nrdI"
                     /locus_tag="J8V14_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBA0921106.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4846..6828)
                     /locus_tag="J8V14_000987"
     CDS             complement(4846..6828)
                     /locus_tag="J8V14_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0921107.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6842..7738)
                     /gene="murQ"
                     /locus_tag="J8V14_000988"
     CDS             complement(6842..7738)
                     /gene="murQ"
                     /locus_tag="J8V14_000988"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBA0921108.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7741..8802)
                     /locus_tag="J8V14_000989"
     CDS             complement(7741..8802)
                     /locus_tag="J8V14_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBA0921109.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9013..9540)
                     /locus_tag="J8V14_000990"
     CDS             complement(9013..9540)
                     /locus_tag="J8V14_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA0921110.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9607..11109)
                     /locus_tag="J8V14_000991"
     CDS             complement(9607..11109)
                     /locus_tag="J8V14_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBA0921111.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11367..12488)
                     /locus_tag="J8V14_000992"
     CDS             complement(11367..12488)
                     /locus_tag="J8V14_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBA0921112.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12614..13555
                     /locus_tag="J8V14_000993"
     CDS             12614..13555
                     /locus_tag="J8V14_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBA0921113.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13705..14601)
                     /locus_tag="J8V14_000994"
     CDS             complement(13705..14601)
                     /locus_tag="J8V14_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBA0921114.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14870..16210
                     /locus_tag="J8V14_000995"
     CDS             14870..16210
                     /locus_tag="J8V14_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0921115.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16428..16871
                     /locus_tag="J8V14_000996"
     CDS             16428..16871
                     /locus_tag="J8V14_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBA0921116.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17375..18793)
                     /gene="nhaC"
                     /locus_tag="J8V14_000997"
     CDS             complement(17375..18793)
                     /gene="nhaC"
                     /locus_tag="J8V14_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBA0921117.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19196..20419)
                     /locus_tag="J8V14_000998"
     CDS             complement(19196..20419)
                     /locus_tag="J8V14_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBA0921118.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20450..21289)
                     /locus_tag="J8V14_000999"
     CDS             complement(20450..21289)
                     /locus_tag="J8V14_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA0921119.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21306..21986)
                     /locus_tag="J8V14_001000"
     CDS             complement(21306..21986)
                     /locus_tag="J8V14_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921120.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21988..23061)
                     /locus_tag="J8V14_001001"
     CDS             complement(21988..23061)
                     /locus_tag="J8V14_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0921121.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(23708..24379)
                     /locus_tag="J8V14_001002"
     CDS             complement(23708..24379)
                     /locus_tag="J8V14_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBA0921122.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24466..27144)
                     /gene="mgtA"
                     /locus_tag="J8V14_001003"
     CDS             complement(24466..27144)
                     /gene="mgtA"
                     /locus_tag="J8V14_001003"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBA0921123.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27803..28240
                     /gene="ndk"
                     /locus_tag="J8V14_001004"
     CDS             27803..28240
                     /gene="ndk"
                     /locus_tag="J8V14_001004"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBA0921124.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28262..29128)
                     /locus_tag="J8V14_001005"
     CDS             complement(28262..29128)
                     /locus_tag="J8V14_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0921125.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29288..31381)
                     /locus_tag="J8V14_001006"
     CDS             complement(29288..31381)
                     /locus_tag="J8V14_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBA0921126.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         9281 a         6682 c         5667 g        10378 t
ORIGIN      
        1 cgttattttt ttatcacaaa aattatctta cttattgtaa ggtaaagctt tttttaattt
       61 attgcccgat aatatataga aataaggtct agttactgtt ttttttaaat gattaataaa
      121 taacaacttc tttctatgaa attgcaaagc attcatcttc ttttccgcta acgatatatc
      181 cgtcagtgct tggtacactt tactaaaaag tatgagattg ccgatatctg gataggtgtg
      241 ctatactaca gaaaaagata tacaatctgc caccgggtag gataaagagg atgatcattc
      301 cattaggtct ttgaagggat gattttcctc tttttgtgta tttttttata gttaaggagg
      361 aatgtaagta tggcatggat ggaattaatc atagcaggaa ttttggaagt attctggtca
      421 acgatgatga aatggagtga tggtttttct aaaatcaatt attcttttta cacagtgatt
      481 ggcatgattg ctagttttta ctttttgtca aaggcaatca aaagtttgcc gatgagtctc
      541 tcttatccta tctggacagg gattggcgca gttggttcgg tcataattgg tgtggtcttg
      601 tttcatgata agctgaatct ttccacttgg ttctttgtgg ggatgctact catcagtatc
      661 attggcatca agattactag cggccactag acacttggaa taagtactga tcagaaaaaa
      721 taagcctttt tatttaatat tcaatttttt tatgaaaaaa taagaaataa gtccaatgac
      781 ctagcgactt ctccactcat caggtgtatg cttgtactat ctatagaaaa ggagtgaata
      841 tttttgaaaa gtctttcggt ccaagaatta tccaagaact ttggtcacaa acaagccctc
      901 agcgatgtca gttttagctt atcccctggc aaaatcgttg gattagtagg tcctaatggc
      961 gctggaaaaa caacgatcat gaaagctatt ttaggactga tccattactc agatggaaca
     1021 atctgtattg gtgaaaatga gatttctccc tctagtcata aagggttgga acaagtagga
     1081 gcattgatcg aatacccggg tatctatcca ttcttaacag gctatgatca tctgaagtta
     1141 ttctctgaaa ccaacgatgt atctgctatc gacactatcg tgaatcagct caagatggaa
     1201 aagtacattc acaaaaaagc gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt
     1261 gctcttgcct tactgaacca tccgtctatc gttattttag atgagccgat gaacggtttg
     1321 gatccacaag caacaagaga tgtgcgaaaa atcattaccg acttggctac tcagggcacc
     1381 tctttcttga tctccagtca cttactcagc gagcttgaaa aaatggtcga tgagctgatc
     1441 ctgatcaatc aaggaaagat catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg
     1501 caagatttca tcgttatctc tacaacaaat gatctagctg ctcgaacgat cttgacagaa
     1561 gctggatata ctttggaaga tcctgataaa atcaagcttt taaaagaaaa cgaagaatta
     1621 ttaggaaccg tgatccagct attgtctgca caaaatatcc atgtgacaga cgtccagcat
     1681 ctgacgaatg atttggaatc ttctctattg gaaattctgg attcagtaaa gggggaagaa
     1741 gaaaaatgac attgcttgta aaacaagaat tatttaaatt gatcaagaaa aagtcaactg
     1801 ctgttttatc tgttctatta gttgttttgc tcataggaac agcacttcta gccaaaaaat
     1861 acacgacgat cattgatcca gttgaaatga ctgctcagct ttttagtgcc acatcttgga
     1921 ttgtctttat catgattgca gctgcaagca cgatcatttc gatggaagca caatatggta
     1981 cattgaaaaa tctcctttat cgaaagtatt ctcgaggaga aatattagta agcaaatgga
     2041 tcacattagt tatttattct gtttatttat accttttagc tattattgtc actgtgttga
     2101 tgaaactgat tcttttccct tcgatttcct tcacggagaa ggtttctact ggtcaaacac
     2161 tgatccaaag cttgttcact tatactttgg gtagttatat cggtttatgg ctgattttaa
     2221 gtttagtgtt gatgattgct tgctttatca atagttccgg cgcatccatc tcagcaggaa
     2281 tcgtctttta ttttgcttcg tcaatcatat ctggtatcct tattgcactc atccaaaaat
     2341 gggagtggat caaatggaat ccgatcagta tgttgaatct gcaaaaccaa attggaaacg
     2401 aagagatcat gaagcaattg actcatcttt ctactaatca aatgctattc ggtaatttag
     2461 cttacatcgt tttgttctta gcattaggat atctcgtatt taagcgaaaa aatgtatgat
     2521 gactcatcaa aaaagaggct gtgaaagaga cgttcagcct tattgccgat tggctgtctc
     2581 ttaaacaccg tttattctga aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc
     2641 tagtatgttt ttgaataaac gacaggctca caacctctct ttatacttct tttatacttc
     2701 tatacgtgaa gcatcccagt ggcaaataaa accattgcaa tcactccgac catcgtcaca
     2761 cctataagta aaatcttatc taagtggata tgttgtacag gctcgttttg ctccttacgt
     2821 gctttgaggt aaaaataaat gcctggcaag aaaagaacaa gaacaagcat tacttcatta
     2881 aaggctacga ctagcatagc ccatacttga aaggcacttg ctaaaagccc tactaaaaat
     2941 tggacccatt ctttccgctg ataagaaagt ttcatttggt acaatcctaa gaaaagccac
     3001 gtgaagaaaa tagctgctgt accaagtgtg taagcaaagt tataagcact gtcagtgaat
     3061 aataatgaaa gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt
     3121 gccccatttt tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc agccatgttt
     3181 cccattgttt caatcggtaa gatcgtccat gagagccaaa caccaactaa agaaatgatc
     3241 aaaccgccgt tgatcacgac tgctccccat ttaccaacca tcatttcaaa cacatagccc
     3301 atagcaggct gcgccttgat cgtttgcagc tgattcatcg tcattgtccc atatggaagc
     3361 atggaaacca agacgtaaat aataatcaag ccgaatatcc ctagaatcgt tgctttccca
     3421 acatctgatt gtttttttgc tcgtgaacct acgacagagg ctccttcaat accaacaaat
     3481 acccaaagca tcaccatgat gcaactcaat acttgactgg aaaacgggat cggctgtcca
     3541 ttcgcattca atataatgtc gctagaaaag ttatcaatga attgttggat cgaaaacgtg
     3601 ctgatagcta ctacaataaa taaaaataat ggcactaatt tacagaatgt gacgatagca
     3661 ttgacgaaac tagcattttc gactccccga ttcactagcc atgcatatcc ccataaaaag
     3721 actgtactca tcacgatcga tgccagattt tgcccacctt caaatactgg gaagaaatat
     3781 cctaatgcgc tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag
     3841 taaccccaac cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag
     3901 atcccgcctt ttaaatctgg acgtttttta ttgatattat tgatcgacaa ggagagcatc
     3961 aacattgctg ttcctacgag tacccaactg aaaatagctg ggccgccagc cgatccggag
     4021 atgtccgtca taataccaaa tacgccacct ccaatcgctg aaccaattac taaggcagca
     4081 agtggaatta aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa
     4141 ataaaaccgc ttttatcaaa aagtataaca aacctttttt atttttactt aattaataaa
     4201 aactttaata gaaatataat tctaccggct tttttctcat tatacaagca aagcgcctag
     4261 tccctttcca aattacacca ccaaaaaact taaaaaagat gctatactaa aaggaaagaa
     4321 ttggaggatg atccatgaac attttatata tatcgatttc aggaaatacg cgtgcctttg
     4381 ccaaacatct agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag
     4441 ttactttaaa agaaatccat gaaaatagtg attttacgaa agaaacagaa ccatttttta
     4501 ctttcgttcc tacttattta gacggcggaa atggtttaga taacggtgat acagaaatct
     4561 tgaccgaaac gatgcgtgaa tatctggaac atgaagacaa tcataaactt tgtcttggtg
     4621 tagtcggtag cggaaataaa aacttcaata atcagtattg tctaacagcc aaacaatatg
     4681 cacaaacatt tggctttcct ttcttggcag attatgaatt acggggaaca ccatctgatg
     4741 tgacacgaat ttatcagatt ttagcagaaa acaataaata ataaaaaaga agaagctgaa
     4801 aaatagattt cagtttcttc tttcgtttct attgtttcta tcatattaag ctttgacatc
     4861 catcacaggt gcttttgctt ttgctttacc tgttttttcc aagacaaagt tttttacacg
     4921 atccatattg gttaccaaga cgatcatgga tgtttctttg cctgctgctt tgatcgcttc
     4981 taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgcccttctg ttactttcac
     5041 atcaaatggc gtaccgccta agtcaactgt gttgattccc atatgaagca agatttctaa
     5101 accatttggc gttgtgatcc caatagcgtg tttcgttggg aagacagagc tgactacccc
     5161 agtaactgga gaatagatat gtccgttttc tggttctaca aagtagcctt cacccatcat
     5221 cttttgtgca aaaactggat cacttgcttt atctaaagaa acaactgttc cgtcagcaac
     5281 tgatgagagg atagtcacac tttcagctga agtttgaggt actacttttt caggcacttc
     5341 atttactgtg gctgtttcag tcgctaattc ttctttttct aaacggtctt taggaatacc
     5401 gaagaagaat gtagcaacga agccaccagc ataagctgcc aataatccaa ggatgtagcc
     5461 taaccattta ccgtcagaga tcaatgggat caaagcggct cctgaaggtc caatagcgat
     5521 agccccgaca tttccgatca ttccgatcac tgccccgccg atacctccac cgatacaagc
     5581 agtaataaat gggcgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc
     5641 taggatcccg actggtaaag ctcctttgat catttctgtt aattctttat cttttttgca
     5701 gcgaatccat aatgccaatg ctgcacccac ttgtcctgca ccagccatcg caagaattgg
     5761 aagcaatagc gtacgaccag tttcatcaat catttgcaca tggattggag tcaaaatttg
     5821 atgcagtccg aacataacca tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc
     5881 tccaccaacg ccaagtaccc agttgatccc accgaccatg ccatcagaaa tgaaacctgc
     5941 taaaggcatg atgaagaaaa tttcaatcag accgatggca atcaatgaaa gagttggtgt
     6001 aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa
     6061 tagccaaaca gcgaagatga cacctaggat cccaccttgt ccagctgcaa gagcttcacc
     6121 agtaaataag tttttcagtg gtgcttccgg attcatccct gtcagcatca caacggcccc
     6181 gataactcca ccaagagttg gtgttgcacc aaaaacttgt gcagcattga tacctgtata
     6241 gataactagg tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg
     6301 ccatgttgct gcatcaagtg ttccggctgt gactaagttt gctaaaactg cggcaatccc
     6361 tgccacgatc ccagtaccga caaatgcagg aatcattggc acaaagatat tagaaatgtc
     6421 ttttaatacc gatttgattt tagatggttt ttgtttcgat ttttgtgccg ctttcatttc
     6481 ttgtgcccgt tcgtttacac ggtcttttcc agatgctgat gaatttgctg tagctgtact
     6541 tacagaagaa agtttttctc ctaatttcac gcctgccaaa tcaaccattt gctgtgctac
     6601 tttgttcact ttcccaggtc cgatgatgac ttgaagctgt tcatcttcta ctacaccaag
     6661 cactcctgga atcgctttta attcagagac atttacaaga tcatcattga tgatcttcat
     6721 acgcacacga gtcatgcagt ggacaatgct ttctacattg cccatgccgc ctgccccttt
     6781 ataaatttgt tttgccagtt cttccaaagt cagttcgttt tttgccatgg tcttttcccc
     6841 ctcataaggt cccttttaca aatccgtttg cttcagctaa ttttgcttga gcttcctctt
     6901 tatcactatt tgtcaaaatc atgacgatag ccagcttaac attttgatct gcttcttcaa
     6961 agtacttttc agctgtttcg taatcagctt ctgtcgcttg catgatgatt cgttttgagc
     7021 gttcgattag tttttcattt gttggtttga catcgaccat caagttattg aatactttac
     7081 ctataccaat catactgata gtagaaatca tattcaagat aagtttttgt gctgttcctg
     7141 acttcaatcg agtagagcct gtcaaaaatt ctggacctgc atcgacttcg attgggaaat
     7201 ccgcgtgttt actgatttcg gcttgtttat tgcaagaaat cgttcctgtg acagcaccga
     7261 tcgattttgc gtaatctaat ccaccgatca catatggtgt acgaccgcta gctgcgatac
     7321 caataactat atcatttttt gtcaaattca gcttgattag atcttcttgc cctagctcat
     7381 aagaatcttc ggccccttcg acagctacag tcattgcttc ttctccacca gcaattagac
     7441 ctatgaccat ttccggttct acaccgaatg tcggtacgca ttcagcagca tctaacacac
     7501 ctagtcttcc acttgttccg gctcccatat aaatcagacg tccacctttt ttaaaggact
     7561 caatcgtgtt agcaatcact ggttcgatac ttgtcagttc tttttcaacc gcagcggcca
     7621 ctttctgatc ttctttgttc attagttgaa cggcttgaga aatactcatc tcatctaatc
     7681 caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt
     7741 ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt ggttgatcaa
     7801 tggtagctct tctttgataa ctttagctac tacattcact ttttcatccg ctggcaaatc
     7861 atatttggtt aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat
     7921 agaccctttc atacgcgtag ctgtatttct tgctgggata ggcggaatct ctcgaaaacg
     7981 tgcatctacg cttcgtatga catctcttgc atcgtcttgc cgattcgtat gcttccctaa
     8041 tatatattcg taaaattctt catctacagg ttccgtatgg agcaagactg tctgttcttt
     8101 ttggaaagaa gcaaattgct cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac
     8161 aatatccgtg ttacttgcta atagatctag agcgcctgca agaggatgga tccctcgatg
     8221 ttcttccaaa gttggcaatc cttgatatag tgggcctctc aactctgtat ctcctggtac
     8281 aaaaccttgg acaaggagac cttgaatttt gagccattga ttttttttct gataccattc
     8341 tttgtccagc cctgtttcgg gacgtggata ataattatgc caagcttcaa tctgtgagaa
     8401 atcagcttct gcttctttca attcatcgat atctttaggc gtgatcgtgc tagcattcaa
     8461 actgattttc aattgatgtg accagtcggc aatctgctga ttggaaatat gataatccat
     8521 tcttaaacct gttacaccaa ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc
     8581 tcgttccaat gcctctcctg aaatatcgac catcaattct agtttattct ttttcgcttc
     8641 gcttcctagt tcgatcaatc gcttttgata tgccgaaaca tcatcttctg gtatatgcat
     8701 ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc
     8761 ttctgacaaa gaatcgttca taaatattga aaagccaaac atattatccc tctttttctg
     8821 tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt
     8881 tattaaaata attaaatttc atttctaaag gttgagttta tattatcaaa agaattgtca
     8941 taacctaaaa aaggcactgc taagcaaaag cagtgccttg aaatatttta tttaaataac
     9001 acaaaaacat aattactcat aagaaaccaa ttctgtatta tctaccatga atgtaaagaa
     9061 cccctcatta ggcatcggat gaaaataagc attgatcact cttatcaagg cattatggca
     9121 aactaacaga tagacatttt cttcgtcttc gatgcattct ttcaataaag gaacgatccg
     9181 tgcatacaca tctaagactg actccccatt tgggaatcgt acagcgaacg ctaaacgtgc
     9241 tttttgaaag ttctcatctt tgcttggcag tccatcataa tccccaaaat ccatttctac
     9301 caatcgttca tcaacggtca gcggcaagct gagacgatca gcaactgctt gtgctgtatc
     9361 tcttgctcgt tgaagagggg aatggatgat tttcgtgatt ggaacaggca aatctactac
     9421 cttttctgct agtttctgtg cctgcatctg tcctttttca gtcagcggca catctgcccg
     9481 tccgcaaact aatccttgtg cgttccaagt tgtttctccg tgtctcgtta cataaagcat
     9541 gtgattctct ccttcgttgg caaagttccc cttaaccata ccttgttgtt cttgagatta
     9601 caagggttag attctctcaa gagaaataat ctcagtagat actttatcca ggaaggtttg
     9661 atcatgttcc actagtaaca tggcaggttt cacgttcaag atcagttgtt ctaattgttc
     9721 ttgattgaag acatccaaat aattcaatgg ttcatcccat gtatatagtt cagctggctg
     9781 tgataaagat ttagccaatt ccactttttt ccgttggccc atactcatct gctcgatctt
     9841 gttatgaaaa acatctcttt ccatcccaag ctttcggagg ttgttcaaaa atgcttggta
     9901 gtctacttgg tttttctctg caaattccgc caacgttcct cgattgtctt cataattttg
     9961 gcttgcataa ctaatgctca gatgttttgg ctggtatttt tctcctataa ccttgccgtt
    10021 gaatgccccc agaagataat ggataatgga tgactttcct gcaccgttag gacctgaaat
    10081 cgccacccgc tgatgaggct cgattgtaaa atgaattggc tcgaataaca gattttcata
    10141 ccctaattga agatcttcta cgcttaaaag tcgcttatgg tgagacgctt ggctattcat
    10201 cgtcaaagaa tcgatatatt cgatatcttt taatagtttt tctttctcgc tgatctgggt
    10261 ctccatccga ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat cagcacccac
    10321 tgctccttta ttcactcgtc tagattcagt atcgatgaat ccgacttgtt tctttgtttt
    10381 atctccttct cgggaacgag accattcggc tttttcagct gctgttttct ttagcctact
    10441 gacttctttt ttcaattttt cattttgagc catttcaaat tcatcacgaa gttttttctg
    10501 ttcttcatag atagagaaat tcccttgata aagttccagt tgactttttt cgattgctaa
    10561 aacatggtcc actacttcat cgataaatcc ccggtcatgg ctgaccacga tgaagccttg
    10621 ttttttcttt ttcaaataag ccgctacttg ttttctacca gagatatcca aatgattcgt
    10681 tggttcatcg attaacggga aatgagtgtc atccacaaat aaaagtgcca gtaggacttt
    10741 tgtcttctct ccccccgata gtgtgctgaa ttctctccat aagatttcag gatctgtttg
    10801 catcaattgg agctctcttt cgatttccca tatctcaaaa tccgtaatat catttaacac
    10861 gtaataggtt aaacgttctt tatcttttgt ctgttgcggg aaataggcaa attcttgctg
    10921 atggattact tgcccttgat aaggtagttt gttttgtaga atattcagta aagttgtctt
    10981 tcctcgaccg tttcgtccga taagtcctag tttccattgt gtgtcaaaat ttagatttgc
    11041 ttgttcaaat aataatgtgc cttggctgtc gtagccgaat gtcagatttt ttatttcgat
    11101 tttagacatt ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga actggctaaa
    11161 gaggatgcaa gggttggata cacacaaaaa gacccataca tcgaaaaaac caagccaatc
    11221 ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg cagctttcat
    11281 caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt gaaattacga
    11341 tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt
    11401 gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc ttcttctaaa
    11461 cctattggac ccaaatgaat acttaagaca attgggagaa aatcagaaag tccttctagc
    11521 tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt tgcaagcact
    11581 gctacaccgt atggaacttt tccgtggatc gtttgctgat cgatccgccc ttctcctgta
    11641 atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct gactaaatcg
    11701 aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc gcctgctgct
    11761 ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg ctgagcaatc
    11821 tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca
    11881 aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc agcgataatc
    11941 aattttactt tagaaacaag aacgtttctt cgatcaaaat gaagtttttt agtagataat
    12001 aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag taatcctaga
    12061 ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac ttcttgaagc
    12121 agtgcatctt taataagttc tcctaaggca taactggaag caagtcggat agtttgatcc
    12181 gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac tgcggtgttt
    12241 tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa gagatccatc
    12301 ccatcaatgg tacgccatgt tcccctgagc gcgtaataga ttgcttctgc agtattttct
    12361 ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca gccatttaga
    12421 actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat tgccgccaaa
    12481 atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca tttttttcaa
    12541 acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc attgaataaa
    12601 ggagagaaaa aacatgaaaa tcgcaatagc tggggccgga gcaatgggca gccgaatcgg
    12661 attgatgcta catcaaagcg gaaatgaggt cctcttgatc gatcgttggc ctgctcacat
    12721 tgaagccatt cgtacaaatg ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa
    12781 actgccaatc tattcaccag aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt
    12841 agcattaaca aaagccaatc aattagatga tatgttttgt tcgatccaat ccattattac
    12901 tgacaatact tatgttttgt gtttactaaa tggattaggt catgaagatg tactggaaaa
    12961 atatgtgcca aagaaaaata tcttatttgg gatcacgatg tggacagctg gtctagctgg
    13021 tcccggaaaa gtcacattgt taggtgatgg agaaatcgaa ctagagaact tggaaccaga
    13081 aggcgaagca tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt taaatccgat
    13141 ctatagccat aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg gaacattgaa
    13201 tggtttatgc acgattttgg attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga
    13261 gagcatggtt cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag aaggaatcat
    13321 actagatcaa gaagaagtgt accaacatat tgcttctact tatgatcctg acaacatcgg
    13381 gttgcattac ccatctatgt accaagacct gatcaaaaac catcgtttga ctgaaatcga
    13441 ctacatcaat ggtgccattt ggcgaaaagg acaaaaatat gatattgcta caccatattg
    13501 tgcctttttg acccaattag tccatgcaaa agaaggaatc cttggcgcag aataaaaggc
    13561 tctctgatga gaatcagctg aatcagcagc tggttctctt tttttaagcc caataaaaga
    13621 tcaaaaagaa aacacaactc tcatgatgcc ataagaattg tgcttgtttg tttcagtaca
    13681 taatctcttc aaaatgtagg ttttttattt gttttcaaca gaggagaggt catattcgta
    13741 ttgctcataa atagtggaat cgttagtgat aaatcgtttt gcccaatttt ctgcatcata
    13801 aaattccaat agctctgata ggattggctt aaatggtgta tgcagaaatt ccggtaattc
    13861 atctaaatca cgttctcgaa gcaaaaaaat attttcagga cgataaaaat gataatgagc
    13921 aatcgcttga ttatccgtca ctagtttctt ttgaaaatac atggattcaa atgtccgcaa
    13981 agtcactcct tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt
    14041 tttcaggtac tcttcataag taagatatcc aggttgttca ttccctcgat gtggaagaat
    14101 gaaatagttg gtcgtaacat ctctttttat aaattcctgc ttatagcttt ctgctcgttc
    14161 ttttctccca ttattcgttg caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa
    14221 attcatatgt ttcgaataaa atgaactatt atgtttcagc ccaagttctt ttgcttccaa
    14281 aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct tcagcatcgc
    14341 ctcgtgttct gctttgaagt gattccaaaa aaataacaca atttttgcat tggtaaatgg
    14401 gcgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga tcagctgata
    14461 atttgccaac tgttgtctag ctgatttaat gaattccgac tgtagtaaat cttttccgga
    14521 ccaaacatct aaagagcgga tggaaggcaa aaaataaagt gatttatttt ttgcaactag
    14581 cagtacgtca tttttgttca tcttttccgc ctcactttct ctgtcacttt ttcctatgat
    14641 agcctgttta aatagtaaaa agccagtaaa acgtttccta tatttgtatg aaaatttaac
    14701 atttgtatga aaatatgatg aaataaatca ttcttctatt aaaattcgcc taaaagtttt
    14761 ctgaatagtt gtaattgtct gaaaatttgt tatcattagt cattgttaaa tattgctatt
    14821 tattcttaat ataaattttg attgtgaatt taaaagaaag aggtcattta tggaagataa
    14881 aaaattgaaa aaacaactta gctccagaca tatcaccatg ttagctttag gtggagcaat
    14941 cggtgcaggt ctatttaaag gaagtggcga agcgattggg atcgccggcc cttctgtatt
    15001 gattgctttt ttgattggtg ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat
    15061 cgttttaagc ggtggcgaca cgcatcacgg attatctggt ttgatacgcc cttatctagg
    15121 tgctcattct gctgatttta ctgattgggt ctactggtct atgtggatta tcaatatcat
    15181 cgctgaagct gttgcagctg cttccttttt gcagttgtgg ttcccgcaca ttccagcctg
    15241 ggtatttgtt tttatgttgg ctgttttaac tacgatcatc aatctttatt cggtacggtt
    15301 attcgcagaa acagaatatt ggctggcttt tgctaaaatc agtgtcatta tcttactgat
    15361 catttttgct acttatcttg tcggtcagca aatgttgggc acaggcgttt tccctaccct
    15421 tcagcaattg actgaccatg gtgggtttac tcctcatggt atgaagggaa tcatcagttc
    15481 tttgcttgta gttatctatt cttacggcgg ttctgaactg attgctatca cggtaagcga
    15541 agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat
    15601 ttccttttac atcattcctt tgtttttgct tttgattctt tttccttgga atactttagc
    15661 aggcacgaat gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc catttgcagc
    15721 ggatatcgtc aattttgtga ttgtccttgc tttgttctca tctatcaatt cgggtgtgta
    15781 tgcatcttca agaattttgt tcttccgttt gaaggatcga aaaggttcct ctagaaaact
    15841 agctgtttta aacaagcatc aagtaccgca acgtgctgtt ttattttgta ccagtgttct
    15901 ttatcttggt gtagctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg
    15961 ttccctttcg tatacagtat tattgatctg gatcattatc agtgcggcat cctttgtact
    16021 ggcactaaga aaaggaacat tatgggataa aggaatcagc ttttttgcat tagctgtctt
    16081 aggattgatt ttccttggta tcctgttcac gaactccatt ggggtaactg tattgactgg
    16141 cctgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac
    16201 gaatgaatga aatcgataga aaattttatt ttatttatgt ggaatagatg gagctattga
    16261 agaaaggagt tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa cagtctcatg
    16321 acctcttggt aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg ttctccatgc
    16381 tcatgacatg atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt
    16441 ggaagttttt gttgatgatc ggtgtttcaa cggtagttat gtttggtatg atgtacttga
    16501 atgtctatgc gctcgatcat atcttcttca gtcgtacacg agtattcatg gcgttgatga
    16561 tgggtgcagt catggcagtc atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa
    16621 agataaatgt tatgatctta gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg
    16681 tacgaagcca aactactgtc aacgacacag cttggatgaa ggccatgatt cctcatcatt
    16741 ctatcgctat cctggcaagt aaacgagctg atttaaaaga tcctgaagta aaaaaattag
    16801 cggaagaaat cattcaggca caagaagaag agatcgacaa aatgaaaaaa atgatcaaag
    16861 aaatggaatg accattatcc gtccaaataa aaaaagagga tgttccagaa gttatcagct
    16921 tcaagaaata agaagaagct acttctggaa cgccgtgaat aaggggttgt gaaaaagctg
    16981 ccctgcatca agtaataaga acagccaaac aaaaatagct tctcctattt ttcgtttggt
    17041 taggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtaaaatca
    17101 gcgttctgac ctgagtatta gagagacaaa tggaaaaata gttcctcata ttttgccatt
    17161 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa
    17221 gtcgcttagt ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca tttttgtata
    17281 aatttggctt aataccgaga aactggcttt tgaataccgt ttattcgtaa aagggattgt
    17341 gacaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc gctgtcttgc
    17401 ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg aaggcactga
    17461 ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag
    17521 gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact aagtagttga
    17581 ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat cctgcttttt
    17641 cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt tcaccaataa
    17701 aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca
    17761 tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc aacaatccac
    17821 ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg attcctccgc
    17881 ggctaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct gattcgatga
    17941 ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt cctactgcta
    18001 cagcgatatt caacaaaatc gttaaaattg ccggcacctt tttccatgca cacagtaaca
    18061 ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct agaacatcag
    18121 tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa atcatgaata
    18181 aaaagaaaga aacaacaaat gccgggacag ttgaccacat catatgcttg atgtgtttga
    18241 acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat ggagacatct
    18301 tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga tttaagccta
    18361 gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta aaggcactcc
    18421 ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac cactggacac
    18481 tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt atccatacag
    18541 caatcaatga accgactaaa ataaaaataa agattgggat gattcctggt ttgatgccat
    18601 tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca tacaacatga
    18661 ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg acaacgccaa
    18721 tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa cttggtgttc
    18781 ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa aaaagttaag
    18841 atatcggatt actccgccgc aattcatccg cgattcctcc tttataaaaa taaaaagacc
    18901 tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac agatctgttt
    18961 ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc aattaggctt
    19021 gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa
    19081 agttgacttg tacgaagttt accttccata agatcatttg tcaatgaaaa agaaaatatt
    19141 ttttcatttt tctgaaaatt atttttataa ctttcacaca caagattatt ttttactaga
    19201 aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc ttgattcttt
    19261 ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa
    19321 tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc
    19381 cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg acttggctga
    19441 catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt tcttcatctg
    19501 gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga gtaacgatga
    19561 catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta cttatcgcag
    19621 cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc cgatttcctg
    19681 cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct actcctccgt
    19741 ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc
    19801 cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt gctgctgctg
    19861 catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa gattctgtcg
    19921 ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc tcaaaaaaac
    19981 cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc cctggggtgc
    20041 catatacacg gatttctcca ccagtttcat caatgaattg cttcactatg ctggcggcta
    20101 atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct ggtaaagcat
    20161 cgaattctgc caaaaaggca attgtgggac cgggtttatt ggaaatataa cgtgcatcaa
    20221 aggctgttcg gtgtccagct acttcttttt ctacccaaaa cctttctttt tctaattgtt
    20281 taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt tcgtggatat
    20341 ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc aattggtttg
    20401 ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct tattttctcc
    20461 cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt actttgactg
    20521 tgtcttctgt ttgataagcg tctactattt tttgatacgt tttattgttt tcatcttctt
    20581 ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt tctaagaaga
    20641 tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg atcactgaaa
    20701 tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa atgtctagat
    20761 tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc ttatccactt
    20821 tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta acatcattag
    20881 ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg ttagaataaa
    20941 ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg tgttctttat
    21001 tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat ccatctgcta
    21061 aagccacatt tggttgattg taatctgtaa atttcacgta ttccaaatta atgttctcct
    21121 ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata atccctagtt
    21181 ttacgatagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac cctgccaacc
    21241 aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt ttttctcctc
    21301 cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact taaaaaaact
    21361 aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg attataccct
    21421 ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat ggtagtcagt
    21481 ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa accttctaac
    21541 aaatacacac gtaaaatgat accgattgga ctggttccca tcgcttctgc cgcttcgatc
    21601 accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact
    21661 ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac taagaaacga
    21721 gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc
    21781 agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg gcgtgtcact
    21841 actaacgtca cacctaagat gattccaatc atcccgccaa tgaatgctgt ccaaaaagac
    21901 atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata aacattgggc
    21961 aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt tgatacgact
    22021 atctaaatcg tttttttgta aaatatttat ttttacacca gatttgatca aataatccaa
    22081 tgcttttttc cgctgtttgg tttctccagc taaagtaacg atcaaactcc ctaacggcgt
    22141 ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat atttgctgta
    22201 cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt ccaccagcca
    22261 tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta atgcttggtc
    22321 aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat tgctgaagat
    22381 ctggatgctg ctaccttttt caacgattct tccatcttcc ataaccgcta ccttgttgca
    22441 gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac ctaactggcg
    22501 attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa gtgcactggt
    22561 tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga ttgctactcg
    22621 ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt ccaaaccgac
    22681 aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc cagaatactt
    22741 taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga aatgctgaaa
    22801 gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct gactcagatt
    22861 cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca tcacacgaac
    22921 taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc ctttttcaat
    22981 cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga
    23041 aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa
    23101 atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat gttcaagctg
    23161 tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc ggtcgcagcc
    23221 gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc caatagtcgt
    23281 tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc gtacttacca
    23341 tttcaatgca aataatattc aaaaaaaaag tccttttact aacataaact atgtcagtaa
    23401 aaggacgacg tatcgcggta ccaccttttt tcggaagttt ccttccctca agcccaacat
    23461 ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca gttccgaatt tgcctactta
    23521 tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt ctcaccagcc
    23581 agagcctctc tgcaaagttt ccaaacttac tttccttttc aacgaaatcg atattttgtt
    23641 gttgggatta atctacatga ggaaaactga aaagtcaagt acgaaattca aattattttt
    23701 tctatattta tttttctgtt tttttattta acatttcaat aacagttcga agatcttttc
    23761 cttccgcaag aagtttcaat ttttcctttt cttcgacggt ataatttcgg acaaaactgg
    23821 aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc agctgttgct cctactaaag
    23881 aatcttccac tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa gctgtcagat
    23941 aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt
    24001 aatcagctaa tttaaagtga tccaagatat ctgttgctct tttcgtaaca gtggtcgtag
    24061 caatgcctag tttcatatga cgttctttta agtctgccaa tatttctttt gcatatggtt
    24121 ttaagtggat ggctccgctt gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga
    24181 cttcctgtac tttttcaggg ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc
    24241 agttttttcc gccccaagcg tcaacgattt cttttgggat atggatatca aattgctgaa
    24301 atccccaaag ccagccatca cgatacgttt tttctgtgtc aactaacaaa ccatctaaat
    24361 caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac atagtttagc
    24421 acgactggtc caaaaagaac agaagcaaag aaccagcctt ctcgtttata agagttcttg
    24481 gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag cgatgactgt
    24541 agcaaataac cataaccagt aattgccagg aagtggcatc aagcctaatt cagcaccaaa
    24601 acttgtaaaa ggaaggacag aaccgacaat aattcctaat gtagtgataa caaacatggc
    24661 aaatgttgcg ttgctttgga taaatggtac tttcggtgta cgcagtgcat ggagcactaa
    24721 ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg aataatgcga tgaaaatcac
    24781 tcgctgtgaa ctatccaatg tatggaaact tccgcccact acagcaggac aaataacaaa
    24841 atacatcaac aagtaagtag taatatcaaa tatcgaactt gttggaccaa agtaaaccat
    24901 gaattcccca atactagatg cattccattt ttttggctcg tccaaatatt ctttatccat
    24961 ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa acaaaagttg
    25021 taaaggcagc attggtaaaa atggcaggaa cgtacttgca atcagcacgg aaaacatatt
    25081 gccaaaatta gaacttgctg tagccttgat atatttcatg atattcccaa aagtttctcg
    25141 tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc
    25201 agattctttc gcaatatcca cagctgtatc tacagaaata ccgatatcag ccactttcat
    25261 tgcaggagcg tcattgatac catctcctaa aaatccgaca gtatgaccat ttttacgtaa
    25321 agctgtcgtc aaacgtgctt tttgctgagg attcaatttt acgaatactt catggtcttc
    25381 agctacttgt gctaattcat gatcactcat ttccgagatt ttctcaccgg taaccaattc
    25441 attgatctct agacctactt gacggcacac tgaacgtgta actaattcgt tgtccccagt
    25501 cagaactttt acagctacac catgttcttt cagagctttc aacgcttgtt tcgttgtttc
    25561 ttttggtgga tcaaggaaag caagataacc aatcaaaacc atgtctgact catctttgac
    25621 tgaaaattct ccaacgacgc ttggattggt cttttgtgcg acaccaatca ctcgtaagcc
    25681 atcttcatta agtgactgta cttttgcaag tacggcttct tttcgttctt ttgttaatgg
    25741 tgtaacagta ccgccaagat caataaattt agaaatgttc agcatttctt caatcgcacc
    25801 ttttgtgatc atttgtgttt taccagctgg atcagcaacc acaacactca tacgacgacg
    25861 ttcaaaatca aaaggaattt catccacttt ttgataattg atcttatctg tatctaaagt
    25921 ttctgttgct tcatcaatga tagccacatc taacaaattt ttcagccctg tttgatagta
    25981 gctatttaga tacgcatggc gcaaaacacg atcatcttca tttccttcac aatccatatg
    26041 atattcaaga atgattttat cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc
    26101 gattgcacca aagttctgga tggcattcaa atttttgatc actgttcctt ttttggccat
    26161 cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt caggtgttag
    26221 accaactgct acagatagtc caaaaagaaa agcttctagc caatcccctt ttgtaagccc
    26281 attgatgacg ataactgctg gagccatcaa tgacataaat ttgatcagta gcatggatgt
    26341 tttatggatg cctaattcaa agcttgattg aattggtttt tcagatagcg tttctgctac
    26401 atgaccaaat agtgtttcat ttccaacaga aacaatgatt ccttccgcac ttccactgat
    26461 gacattactt cccataaaga caagattttc gtaacttgtt tcgctttgct gatcgtcttc
    26521 acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg cttgggaaat
    26581 gaataaatcc tttgcggaca ataaacgcat atccgctggt atcatatccc ctgctgaaag
    26641 ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtgtact ttccattacg
    26701 ttttacagct gccgttactt tcacaagtga tttcagtttt tcagccgctt gattggaacg
    26761 tacggactga atcaacgtca ttgtcccgct gacgataacc atagtgaaga taatggctac
    26821 accgaaaaga tctttatctc caggagctgc taatacatat tccgtgatga atgaaataat
    26881 tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat gctttcaata tttctacaat
    26941 gaatggtgtt tttttgccat atgaaatcgc attttcaccg taattttctc ttgaacgttc
    27001 gatttgttct tctgtcaatc catttgctga tgcatggaat agttcaaata attcttgttt
    27061 cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt ccatctcaat
    27121 ctctcctttg tgtccaaaag acagctgttc tcgctgcttt tggtctttag ctattcatcg
    27181 gatcgataga gaaaaaactg aaaggggctg agaacagata gataatagcc ctggcctttc
    27241 ctctagatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc tttactaagt
    27301 aattttaatt catgacttcc aatcattttt cgaatagtta tttttccaat tagaccacct
    27361 cctttttatt tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc
    27421 agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca gatctcatcg ttgagttttg
    27481 acactgtaca acgtaaaaag cagatacctg cttttagcta ccttatgaaa agccttatct
    27541 cgacaattcc tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt
    27601 gtataggagc ctcacctaac aaatcttatt cggttttcct tcgttaatat aacaactaaa
    27661 aaagaggatg tcaaatcaat tttccatgtt tttcatactt tttttaaaat acacacgtta
    27721 tattcctgtc tcacgcggtt ttatttgtct cattttccac ctcccgctat aatagaaata
    27781 tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt attatcaagc cagacggcgt
    27841 gagacgccat ttagtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc
    27901 tgaaatgaag tttgaaacta tgactcctga attagcaaaa gaacattatc agcacttgac
    27961 tgagcgcagt ttttttgatg aactgatcga ttacatgact tccggtcctg tcgtttatct
    28021 tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag atggttggag caacaaaagc
    28081 tgccgatgct gttccaggta cgatccgagg agactacgcg ctgccgggga ctgaaaacat
    28141 cattcacgct tctgattccc gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc
    28201 attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg
    28261 tctagttgct gggttcagca atggccagca gttttttctc taaggacctc cgttgattct
    28321 ttcctaacat atattcgagc actgtgaaga attcttggta gtctttcgtc ttttcttccc
    28381 ctaaagcagc agaaatcaat ctttgataat aatctgccat caatgtttca tagatcactg
    28441 tacttctttc tttatgtgct aagctccgtt tcatatcttc attcacacta cgtgcctttt
    28501 tcaaccgacc attttctata taaaacgaag tcaggttcat taaaaaagta aagagatact
    28561 gctcatgatt gttcaactgt gctaatcgtc gcattctttt tttgattttc aaataattgt
    28621 tctcaatgaa ccgatcactg aatatccaag gaaaatcagc tagaatagat agttcaaatt
    28681 gaccatagtc tggtatctgc atcaagtatt cttcaatgat tcggcattca taacgtaaaa
    28741 attcaaattc tgtctcagat acttctgttt tttcgaccat gtgaagattc aaaaccaaat
    28801 aatacagatc ttctgttttc ttaaaatgcc tacggttatt gctgatctct ttataaggat
    28861 caaagctgtc ttcttgtacc cgatctagca ttcgtctttt tttctttaac tttattggtt
    28921 catccagctg gtttcgcatc cattcatatt ctgcaagtgt catgttcatt cgctctaaaa
    28981 atgatctcaa tgttacaaat gacatttttt tcactgaatt tgtctcaatc ccgactagag
    29041 aagtcttaaa acagattcct tccgctaatt gggactgtgt caacttcttg ttgactcgaa
    29101 gtttcttgat caattcacca tcatacataa tagttctcct ttttaattta aatttttcca
    29161 cattcaataa atagatacgt taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt
    29221 tttcataaaa aataccaacc ttcaagcatt agaatgttgt ctaactactg aaggttggtt
    29281 tgattgttta ttgattattt ttttgctgtt caacgtctgt tcttgttact ggatataagt
    29341 tggttgttgt tttattacca ttttttgaaa agacacttgt cgaatgttcg cctttttgct
    29401 tttcgattgc ttcattacgt tccgcttcat ttttatagtc atattttttc ggatcaatcg
    29461 tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc ttatctgaaa
    29521 gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcatcg attcctgctg
    29581 ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct aggacggtgt
    29641 atttgggtgt cacaaagttc ccatttcgaa aagcaactac ttgatcatgc tgagaagata
    29701 ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga tgtagtaatg
    29761 tcggcaaaac atcgatttct ccaccgtatt catgattgac tgtcccttta gtataaccag
    29821 gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca tcccatgtat
    29881 ctggatcttt accaagagcg cttgctaaat ctttattatc tgtattcgag atcccaaaat
    29941 gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc aaatacgtaa
    30001 aaaattgttc caatgattga tccagataat gtgccgttcg aacataatta tctactgtgc
    30061 tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa taaggcgtgt
    30121 ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca agatattggg
    30181 cagattctct gaataaatct ttatctaata tcccataacc taacgtttcg tcggattcat
    30241 caaaataaga tcgatcaaag aaattgtcat aacctaagtt tttatataca tgatctctat
    30301 tccaaaaact agctacattc ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa
    30361 ggatcgctgg tgcggcttga aagacattgt ctgacccaag ttctgtgaat aatgagcctt
    30421 gaggtaaacc aaatgtgcca gtttccaaca tattttcagc atcacttgtc tttccctgtc
    30481 ctacttcatg aaagaaattg tcaaagctga tcgttgcttg attctggaaa atactattta
    30541 aaaacggtgt gacttcttgt ccgtctacct tcatattaat caaaaactgc tggaaacttt
    30601 ctaaatggag aacgatcaca tttttccctt tagcgatccc aaatgtttcg ggatttggct
    30661 ctgcataatg tttctttgta taatctaaaa cttcatcaat cccattactg cttgcatgcg
    30721 cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtatac gcatcgattc
    30781 ctagatattt gacaatatat gaacggtcaa atgttcgctg cagcaattgt ggacggttgg
    30841 cttcgctcag cgctagattt cctgaaaaaa taagccctga aagacaggta acagcgatag
    30901 ccatcggttt gccgacaggg ttgcttttga taggtatttt cttgaccttc aaccaaacca
    30961 gtagcccaat gaacacagcg atatccagcc agtagatgac atcatggggc ttcatcaaag
    31021 aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca gatttgactg
    31081 taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa agcgtattta
    31141 aaatatcaat gatcaaaaga atagctaacg ctggctttgg ctttttaata tacaaagaaa
    31201 ggccaaaaaa caataaggta gtcgcaattg ggttgatcca caagataaag tactggatcg
    31261 taccttccac acctaatgaa aaatctgcat agtaggcaat gatcgtcttc agccagaaaa
    31321 agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta agttgtgtca
    31381 cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa atgaaaattt
    31441 cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt ccgtccactt
    31501 ttcacagcat aaaaccgctg cgatgtaagc gattaaacta atgaagagat aaaagtcttg
    31561 tggtgaccgc ttcactccac ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa
    31621 gaggctgtga caaaattctt gtcgcagctc cttatttctt gatgcaggac agctttttca
    31681 caacttcttc cttaaacggt gttctcctgc caactcctcg gccacaagtc acaatattta
    31741 taaaacatga gaagctgttt tttgaaattg ttccttgtgg ctcagagtta aacggcaggc
    31801 tcaagacctc ttgatacacg gtgttcaatc agctgaccct cggtattaga ttatgtcagg
    31861 acgctaattt tacaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    31921 cccaaccaaa cgaaaaatat aaggagctat tttttgaaat tgctccttgt ggctcagagt
    31981 taaacggcag gctcaagacc tcttgata
//
LOCUS       DADQRX010000023        30991 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.023, whole genome shotgun sequence.
ACCESSION   DADQRX010000023 DADQRX010000000
VERSION     DADQRX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.023"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(524..1078)
                     /locus_tag="J8V14_001007"
     CDS             complement(524..1078)
                     /locus_tag="J8V14_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBA0921127.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1204..3133)
                     /locus_tag="J8V14_001008"
                     /pseudo
     CDS             complement(1204..3133)
                     /locus_tag="J8V14_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3439..4155)
                     /locus_tag="J8V14_001009"
     CDS             complement(3439..4155)
                     /locus_tag="J8V14_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBA0921128.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4156..5979)
                     /gene="glpO"
                     /locus_tag="J8V14_001010"
     CDS             complement(4156..5979)
                     /gene="glpO"
                     /locus_tag="J8V14_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBA0921129.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5993..7489)
                     /gene="glpK"
                     /locus_tag="J8V14_001011"
     CDS             complement(5993..7489)
                     /gene="glpK"
                     /locus_tag="J8V14_001011"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBA0921130.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7625..9085)
                     /locus_tag="J8V14_001012"
     CDS             complement(7625..9085)
                     /locus_tag="J8V14_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0921131.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9075..10370)
                     /locus_tag="J8V14_001013"
     CDS             complement(9075..10370)
                     /locus_tag="J8V14_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA0921132.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10702..11379)
                     /locus_tag="J8V14_001014"
     CDS             complement(10702..11379)
                     /locus_tag="J8V14_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBA0921133.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11409..12500)
                     /locus_tag="J8V14_001015"
     CDS             complement(11409..12500)
                     /locus_tag="J8V14_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBA0921134.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(12601..13035)
                     /locus_tag="J8V14_001016"
     CDS             complement(12601..13035)
                     /locus_tag="J8V14_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0921135.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13619..15076)
                     /gene="ascB"
                     /locus_tag="J8V14_001017"
     CDS             complement(13619..15076)
                     /gene="ascB"
                     /locus_tag="J8V14_001017"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA0921136.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15073..16968)
                     /locus_tag="J8V14_001018"
     CDS             complement(15073..16968)
                     /locus_tag="J8V14_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0921137.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17026..18801)
                     /locus_tag="J8V14_001019"
     CDS             complement(17026..18801)
                     /locus_tag="J8V14_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBA0921138.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18851..20347)
                     /locus_tag="J8V14_001020"
     CDS             complement(18851..20347)
                     /locus_tag="J8V14_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0921139.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20541..22163)
                     /locus_tag="J8V14_001021"
     CDS             complement(20541..22163)
                     /locus_tag="J8V14_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBA0921140.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22491..23804)
                     /locus_tag="J8V14_001022"
     CDS             complement(22491..23804)
                     /locus_tag="J8V14_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA0921141.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24049..24273)
                     /locus_tag="J8V14_001023"
     CDS             complement(24049..24273)
                     /locus_tag="J8V14_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921142.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24293..24661)
                     /locus_tag="J8V14_001024"
     CDS             complement(24293..24661)
                     /locus_tag="J8V14_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBA0921143.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24857..25567)
                     /locus_tag="J8V14_001025"
     CDS             complement(24857..25567)
                     /locus_tag="J8V14_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921144.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25585..26082)
                     /locus_tag="J8V14_001026"
     CDS             complement(25585..26082)
                     /locus_tag="J8V14_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0921145.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26119..26571)
                     /locus_tag="J8V14_001027"
     CDS             complement(26119..26571)
                     /locus_tag="J8V14_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0921146.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26706..27311)
                     /locus_tag="J8V14_001028"
     CDS             complement(26706..27311)
                     /locus_tag="J8V14_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBA0921147.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27761..28381)
                     /locus_tag="J8V14_001029"
     CDS             complement(27761..28381)
                     /locus_tag="J8V14_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0921148.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28383..29252)
                     /locus_tag="J8V14_001030"
     CDS             complement(28383..29252)
                     /locus_tag="J8V14_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBA0921149.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29275..29682)
                     /locus_tag="J8V14_001031"
     CDS             complement(29275..29682)
                     /locus_tag="J8V14_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBA0921150.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29701..30990)
                     /locus_tag="J8V14_001032"
     CDS             complement(29701..30990)
                     /locus_tag="J8V14_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0921151.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8803 a         6709 c         5133 g        10346 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
      181 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
      241 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
      301 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
      361 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
      421 aacaacttct cctgtttttg gttcggttgg cttattatcg agaagtagct actgggagtc
      481 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
      541 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
      601 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
      661 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
      721 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
      781 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
      841 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
      901 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
      961 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
     1021 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
     1081 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
     1141 caatgatttc actgttttta tttttcttta cagacactga tattatgagc taatttgaga
     1201 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
     1261 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
     1321 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
     1381 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
     1441 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
     1501 tatccgatca ccttttgcaa agagctgacg ttctttttta ttctccttcc tatttgtaac
     1561 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
     1621 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
     1681 gctttctcct gtttcttcca acccgcaact gatatttagg acgaagtgct tcccgatcga
     1741 aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg atttctccat
     1801 aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg attgcgatcg
     1861 ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa agtaccaacg
     1921 tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca gaaaatgtac
     1981 tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca tcaaagacag
     2041 caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc aaagtcaata
     2101 aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg ataatcaagc
     2161 ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt aaaaatggat
     2221 gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg cttccattga
     2281 tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc gtttcgatca
     2341 ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgcc aggacttgat
     2401 attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat ttagaaatca
     2461 cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc gttccatcta
     2521 ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt tgaacaatca
     2581 ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt aattttgact
     2641 ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt
     2701 cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca tttatcaaat
     2761 caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa
     2821 actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa tcggttggct
     2881 gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc aaccccataa
     2941 atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct tcttgacgat
     3001 ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt ttgctatagt
     3061 ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat tgactaatca
     3121 tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa taaataaggg
     3181 cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta tccttattag
     3241 aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa ataaactttg
     3301 tttattggct attcgactta ctgattagat accagctaca ctcacaaaac ataccccatt
     3361 cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg ttgtcaaact
     3421 ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga taattgctcc
     3481 gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc ctttattagc
     3541 tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt tgattgcata
     3601 tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta cagcgaatac
     3661 gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa
     3721 tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag gacctgttgc
     3781 aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca aataagaaag
     3841 ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga aaggaacgac
     3901 aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag gattcaaatg
     3961 agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc cccaaccaaa
     4021 tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca cagccgcaca
     4081 cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca tgatttgagt
     4141 catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt ctgcaatcaa
     4201 tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa aatatccagc
     4261 aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa acaacagatg
     4321 atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt ctaaaccata
     4381 acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt cttttgctaa
     4441 ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag catcttcttc
     4501 ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt ctaatttagt
     4561 tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt tcgtctctat
     4621 tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg ccatcttacg
     4681 gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc tttctaaaga
     4741 actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa
     4801 cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta ctgtatcgac
     4861 aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt aatcagaacc
     4921 agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat cattcaatgt
     4981 gatatttgct tctggatagc gatgattgat cacatccaat agataatcta catcttcttg
     5041 tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac caaagtaagt
     5101 cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg tatcgaagta
     5161 tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc ctttagttgg
     5221 acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat ctacccaagg
     5281 accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg tcagcaggtc
     5341 acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg ctttcatttt
     5401 acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca ctaaacgggc
     5461 atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt ttttcaaaaa
     5521 cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcatatt tggttcctgt
     5581 cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca tattgaaggt
     5641 tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg ggatgtgtgg
     5701 ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt ccacatcaaa
     5761 tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg aagtcccttc
     5821 tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg actggactgc
     5881 cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt cttgctgcat
     5941 tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct ccttattcaa
     6001 atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct cggcgctctt
     6061 cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc tcgtctgtat
     6121 ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca
     6181 agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc atcagatatt
     6241 cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca gtgtcatctt
     6301 gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta gctttaatga
     6361 tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc
     6421 aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct tcgcttgtag
     6481 agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt ccatctctca
     6541 gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag taaacctttc
     6601 catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc ggtttttcac
     6661 cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg cctggttcga
     6721 atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa attggtgctt
     6781 cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta gaagtgactt
     6841 ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc caatctagct
     6901 gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca tgtgcttgtc
     6961 catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat tcgccttttt
     7021 cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc gttgctgaga
     7081 aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt
     7141 ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca tgatagattg
     7201 gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt atgccaatcc
     7261 cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata actgactgta
     7321 cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg aaatattgcg
     7381 tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg
     7441 agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt gtgattccct
     7501 ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt aaaaaaagaa
     7561 actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg aagctatttt
     7621 ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga ataatgtcgt
     7681 aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc tggcaaaatg
     7741 tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata ccgccgattc
     7801 ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc agactttcta
     7861 aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa agaacattgg
     7921 catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc ggcaattgct
     7981 gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg agcattttct
     8041 gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca aagaaaaacc
     8101 attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg attttcaaac
     8161 gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa acagcggttg
     8221 gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct actatcgaaa
     8281 aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct gctgctgttc
     8341 gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc gactgataga
     8401 gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt ctttttttcg
     8461 taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata
     8521 cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa aaaagcggtc
     8581 gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc tgtagctctt
     8641 ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc tggttcaatt
     8701 ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct aatcgaacga
     8761 tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag ctttcctgca
     8821 ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc agcgcctgat
     8881 actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat tcaatatctt
     8941 ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa ccattgatag
     9001 aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac tgctcttttc
     9061 tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca caccatatct
     9121 ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc aaacgtccat
     9181 ccttcttcta ctgcaattgt cagttggtca agaaggtgga acatctcttt tttgctttgg
     9241 agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt aactttgctt
     9301 tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg gggataaaaa
     9361 atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca ctgattgccg
     9421 actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt gctgacattt
     9481 cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt tgttggacga
     9541 aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt ttgcaagtag
     9601 tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca catctgattg
     9661 tcgggacgag aatgattagc cagcaaaaca agatcatagt tctcctttct tttttccgtc
     9721 tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa
     9781 agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc tagataacga
     9841 aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga tgccaaccct
     9901 tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt tgcattcatg
     9961 atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat aagcgttgat
    10021 tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa
    10081 tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat tactgtcttt
    10141 tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat ccaagaaagt
    10201 tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg aatccatcct
    10261 aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg gcgaagataa
    10321 atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat ctgcattcct
    10381 ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta ttcataactc
    10441 gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat cataccgtgt
    10501 tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc atttctgaag
    10561 agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac gtacctctgt
    10621 ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta
    10681 gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt cgcctaattg
    10741 atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa ccacttctga
    10801 aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt ctgctaagaa
    10861 tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc cagtcactgt
    10921 acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc ctacaacacc
    10981 gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga ctttcccaag
    11041 atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat aaaccgctgc
    11101 ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc gatttctgaa
    11161 atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac tttgcggttc
    11221 taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat tttttactaa
    11281 tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct cgatcttttc
    11341 gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct ctttctataa
    11401 aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg caaataaagc
    11461 tctttatatg ctttttcaaa aggtttcgtt acaaattcat cctcacggtt ttccaataat
    11521 ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat
    11581 ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac taaagggatc
    11641 tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta tttatcagaa
    11701 cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt tgactcacgt
    11761 aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc atattcttct
    11821 ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac cgctgagaaa
    11881 accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc gccccattga
    11941 tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt atttgctcca
    12001 tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt gccaaatgct
    12061 tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc atcaggacgt
    12121 tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc tcgacttgtc
    12181 attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg gattgctttc
    12241 gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc acctggccag
    12301 ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag acctttagaa
    12361 cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt
    12421 gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg taaagtaatt
    12481 gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta acctatcgag
    12541 aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc ttaaaaaatt
    12601 ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt ctttacttgc
    12661 ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac catcgacttc
    12721 cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac cttcgctaac
    12781 tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg ttgatggggc
    12841 ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt ttaatatcaa
    12901 taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat cagctaattt
    12961 attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta acgctttttc
    13021 aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg atttttattt
    13081 tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa aaaagaagta
    13141 gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac accgtgaatc
    13201 agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg aaaatggctt
    13261 tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa caccgtttat
    13321 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
    13381 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
    13441 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
    13501 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
    13561 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
    13621 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
    13681 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
    13741 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
    13801 ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
    13861 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
    13921 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
    13981 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
    14041 ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg
    14101 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
    14161 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
    14221 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
    14281 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
    14341 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
    14401 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
    14461 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
    14521 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
    14581 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
    14641 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
    14701 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
    14761 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
    14821 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
    14881 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
    14941 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
    15001 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
    15061 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
    15121 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
    15181 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
    15241 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
    15301 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
    15361 tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt
    15421 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
    15481 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
    15541 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
    15601 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
    15661 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
    15721 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
    15781 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
    15841 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
    15901 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
    15961 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
    16021 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
    16081 ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat
    16141 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
    16201 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
    16261 ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
    16321 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
    16381 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
    16441 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
    16501 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
    16561 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
    16621 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
    16681 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
    16741 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
    16801 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
    16861 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
    16921 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
    16981 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
    17041 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
    17101 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
    17161 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
    17221 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
    17281 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
    17341 aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag
    17401 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
    17461 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
    17521 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
    17581 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
    17641 cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa
    17701 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
    17761 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
    17821 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
    17881 aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt
    17941 aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga
    18001 aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa
    18061 taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag
    18121 ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac
    18181 ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg
    18241 aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt
    18301 tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt
    18361 aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga
    18421 aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta
    18481 cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc
    18541 ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt
    18601 atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg
    18661 gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt
    18721 cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca
    18781 cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga
    18841 ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa
    18901 tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat
    18961 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
    19021 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
    19081 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
    19141 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
    19201 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
    19261 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
    19321 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
    19381 tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac
    19441 agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat
    19501 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
    19561 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
    19621 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
    19681 cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
    19741 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
    19801 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
    19861 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
    19921 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
    19981 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
    20041 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
    20101 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
    20161 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
    20221 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
    20281 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
    20341 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
    20401 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
    20461 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
    20521 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
    20581 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
    20641 tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
    20701 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
    20761 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
    20821 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
    20881 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
    20941 ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt
    21001 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
    21061 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
    21121 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
    21181 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
    21241 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
    21301 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
    21361 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
    21421 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
    21481 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
    21541 ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc
    21601 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
    21661 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa
    21721 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
    21781 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
    21841 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
    21901 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
    21961 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
    22021 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
    22081 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
    22141 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
    22201 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
    22261 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
    22321 tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt
    22381 gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt
    22441 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    22501 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    22561 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    22621 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    22681 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    22741 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    22801 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    22861 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    22921 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    22981 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    23041 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    23101 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    23161 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
    23221 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    23281 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    23341 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    23401 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    23461 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    23521 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    23581 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    23641 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    23701 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    23761 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    23821 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    23881 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    23941 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    24001 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    24061 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    24121 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    24181 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    24241 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    24301 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    24361 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    24421 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    24481 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    24541 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    24601 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    24661 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    24721 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    24781 aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga atgccgctct
    24841 tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt gtgtgtctaa
    24901 cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc
    24961 cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct tgtgtttctt
    25021 ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc gctccttgtt
    25081 catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca cctaaaggca
    25141 ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc ttttcgatat
    25201 cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata tctacaagcg
    25261 gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct tcaatagaag
    25321 aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga gcaatgactt
    25381 cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct gcctcattca
    25441 ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata ccatttacgg
    25501 ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc actattactt
    25561 ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat agtctgtttg
    25621 gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt ttttacgata
    25681 aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg ttttttgctt
    25741 ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt gatgtttaaa
    25801 tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt cctcagattg
    25861 actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac tcactaggaa
    25921 aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt
    25981 caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg taaatgtttc
    26041 gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa tttcgtattt
    26101 attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa aattccaatc
    26161 ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg cttcttcgtc
    26221 agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga tgaaaggata
    26281 gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt ctttatcttc
    26341 caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag tcgttgtacg
    26401 atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt cgcaaatacg
    26461 tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga tattgctaat
    26521 agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca tttcatccca
    26581 cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa
    26641 taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt cttttacgaa
    26701 aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg tttcttcgtt
    26761 gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat gttggaagtt
    26821 tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa aacaagttta
    26881 taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac tgctcgcaac
    26941 tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac attttcttca
    27001 ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt ccgtttatct
    27061 tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac ttttggtgtt
    27121 ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc ttttcctatt
    27181 tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt atcaaataca
    27241 tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta ttgagccaac
    27301 ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa tcaagtatag
    27361 aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata aaagaatgtt
    27421 aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa tggcgttcaa
    27481 aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc tattcttgtc
    27541 tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac taaacggtgt
    27601 tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga agctgttttt
    27661 ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct cggtaaacag
    27721 cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg tcattgattt
    27781 ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct gttacatcaa
    27841 attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg atctttttga
    27901 atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg tcaccatagt
    27961 gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt ttgtaccgac
    28021 gttgccataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga tatccgtatc
    28081 tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac tcggacacaa
    28141 tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa ggataaactt
    28201 tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat tctgcatagt
    28261 attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc cgtagtttat
    28321 tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa tactttttca
    28381 tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc aatcttatgc
    28441 ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag agacttgttg
    28501 atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa aagctccgtt
    28561 ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc ttctttcaca
    28621 cctgttcctc catggataac tagggggaca tcaactactg catcgatttc tttcagcaag
    28681 ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat agcaatagct
    28741 aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct tgtatatacc
    28801 ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc aagttctcct
    28861 tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt caggatattt
    28921 tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct gtcgattgct
    28981 tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc aaccatttct
    29041 tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata tttagcggta
    29101 tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc tagcatagca
    29161 ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa ctcttctgct
    29221 gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat ctttttattt
    29281 gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt tgcccatgta
    29341 gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct ggttatagag
    29401 gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac tcttttctaa
    29461 atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa cttgttcata
    29521 aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca attgtgtaat
    29581 ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt ttgccagaac
    29641 ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc ctactttcaa
    29701 ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg cggctactgt
    29761 atctacatcg gttgctgtag cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa
    29821 agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg ctcctcggcg
    29881 tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat tggttagcat
    29941 accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa taatcgtacc
    30001 aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa agaaaactgg
    30061 acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc gaaatgccgg
    30121 cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac ccactacgaa
    30181 ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata attcagaagc
    30241 gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca cgaccatgac
    30301 agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat cttcgatcga
    30361 ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa ataagtaccc
    30421 gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac gaatgatcgg
    30481 ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac cgaccaagat
    30541 caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg ttgccatcca
    30601 taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag ccaaaataat
    30661 attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac ccaaatcatt
    30721 cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat gaaatccttc
    30781 agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac cggcactcaa
    30841 cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg ctgatttctt
    30901 ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat
    30961 attttcgatg ataaaattga taacgcccat g
//
LOCUS       DADQRX010000024        30459 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.024, whole genome shotgun sequence.
ACCESSION   DADQRX010000024 DADQRX010000000
VERSION     DADQRX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.024"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(451..1761)
                     /locus_tag="J8V14_001033"
     CDS             complement(451..1761)
                     /locus_tag="J8V14_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBA0921152.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="J8V14_001034"
     CDS             complement(1773..3131)
                     /locus_tag="J8V14_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0921153.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="J8V14_001035"
     CDS             complement(3333..4901)
                     /locus_tag="J8V14_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBA0921154.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="J8V14_001036"
     CDS             complement(4905..5315)
                     /locus_tag="J8V14_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBA0921155.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="J8V14_001037"
     CDS             complement(5312..6235)
                     /locus_tag="J8V14_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBA0921156.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="J8V14_001038"
     CDS             complement(6232..7110)
                     /locus_tag="J8V14_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBA0921157.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="J8V14_001039"
     CDS             complement(7097..8434)
                     /locus_tag="J8V14_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBA0921158.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="J8V14_001040"
     CDS             complement(8437..9540)
                     /locus_tag="J8V14_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HBA0921159.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="J8V14_001041"
     CDS             complement(9614..11122)
                     /locus_tag="J8V14_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBA0921160.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="J8V14_001042"
     CDS             complement(11088..11780)
                     /locus_tag="J8V14_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBA0921161.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="J8V14_001043"
     CDS             complement(11802..12776)
                     /locus_tag="J8V14_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921162.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="J8V14_001044"
     CDS             13076..13948
                     /locus_tag="J8V14_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921163.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="J8V14_001045"
     CDS             complement(14406..16604)
                     /locus_tag="J8V14_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921164.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="J8V14_001046"
     CDS             16836..17828
                     /locus_tag="J8V14_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0921165.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="J8V14_001047"
     CDS             17888..19309
                     /locus_tag="J8V14_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBA0921166.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="J8V14_001048"
     CDS             19302..20801
                     /locus_tag="J8V14_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBA0921167.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="J8V14_001049"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="J8V14_001049"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBA0921168.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="J8V14_001050"
     CDS             22393..23076
                     /locus_tag="J8V14_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBA0921169.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="J8V14_001051"
     CDS             23078..23914
                     /locus_tag="J8V14_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA0921170.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="J8V14_001052"
     CDS             23925..25679
                     /locus_tag="J8V14_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921171.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="J8V14_001053"
     CDS             complement(25955..26296)
                     /locus_tag="J8V14_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBA0921172.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="J8V14_001054"
     CDS             complement(26364..28535)
                     /locus_tag="J8V14_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBA0921173.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="J8V14_001055"
     CDS             28746..29603
                     /locus_tag="J8V14_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBA0921174.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="J8V14_001056"
     CDS             complement(29674..>30459)
                     /locus_tag="J8V14_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0921175.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DADQRX010000025        30273 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.025, whole genome shotgun sequence.
ACCESSION   DADQRX010000025 DADQRX010000000
VERSION     DADQRX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.025"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(158..1642)
                     /gene="guaB"
                     /locus_tag="J8V14_001057"
     CDS             complement(158..1642)
                     /gene="guaB"
                     /locus_tag="J8V14_001057"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBA0921176.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2147..2842)
                     /locus_tag="J8V14_001058"
     CDS             complement(2147..2842)
                     /locus_tag="J8V14_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBA0921177.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(2932..4032)
                     /gene="ychF"
                     /locus_tag="J8V14_001059"
     CDS             complement(2932..4032)
                     /gene="ychF"
                     /locus_tag="J8V14_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBA0921178.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4041..4232)
                     /locus_tag="J8V14_001060"
     CDS             complement(4041..4232)
                     /locus_tag="J8V14_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBA0921179.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4253..5143)
                     /locus_tag="J8V14_001061"
     CDS             complement(4253..5143)
                     /locus_tag="J8V14_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBA0921180.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5130..5897)
                     /locus_tag="J8V14_001062"
     CDS             complement(5130..5897)
                     /locus_tag="J8V14_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBA0921181.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(5909..6622)
                     /gene="rsmG"
                     /locus_tag="J8V14_001063"
     CDS             complement(5909..6622)
                     /gene="rsmG"
                     /locus_tag="J8V14_001063"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBA0921182.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(6854..8755)
                     /gene="mnmG"
                     /locus_tag="J8V14_001064"
     CDS             complement(6854..8755)
                     /gene="mnmG"
                     /locus_tag="J8V14_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBA0921183.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(8772..10169)
                     /gene="mnmE"
                     /locus_tag="J8V14_001065"
     CDS             complement(8772..10169)
                     /gene="mnmE"
                     /locus_tag="J8V14_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBA0921184.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10513..10851
                     /locus_tag="J8V14_001066"
     CDS             10513..10851
                     /locus_tag="J8V14_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921185.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(11019..11777)
                     /locus_tag="J8V14_001067"
     CDS             complement(11019..11777)
                     /locus_tag="J8V14_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBA0921186.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(11795..12613)
                     /locus_tag="J8V14_001068"
     CDS             complement(11795..12613)
                     /locus_tag="J8V14_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBA0921187.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12610..12966)
                     /gene="rnpA"
                     /locus_tag="J8V14_001069"
     CDS             complement(12610..12966)
                     /gene="rnpA"
                     /locus_tag="J8V14_001069"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBA0921188.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13084..13218)
                     /gene="rpmH"
                     /locus_tag="J8V14_001070"
     CDS             complement(13084..13218)
                     /gene="rpmH"
                     /locus_tag="J8V14_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBA0921189.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            13742..15076
                     /gene="dnaA"
                     /locus_tag="J8V14_001071"
     CDS             13742..15076
                     /gene="dnaA"
                     /locus_tag="J8V14_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBA0921190.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15274..16404
                     /gene="dnaN"
                     /locus_tag="J8V14_001072"
     CDS             15274..16404
                     /gene="dnaN"
                     /locus_tag="J8V14_001072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBA0921191.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16633..16875
                     /gene="yaaA"
                     /locus_tag="J8V14_001073"
     CDS             16633..16875
                     /gene="yaaA"
                     /locus_tag="J8V14_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBA0921192.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            16862..17986
                     /gene="recF"
                     /locus_tag="J8V14_001074"
     CDS             16862..17986
                     /gene="recF"
                     /locus_tag="J8V14_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBA0921193.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            17983..19929
                     /gene="gyrB"
                     /locus_tag="J8V14_001075"
     CDS             17983..19929
                     /gene="gyrB"
                     /locus_tag="J8V14_001075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBA0921194.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            19949..22420
                     /gene="gyrA"
                     /locus_tag="J8V14_001076"
     CDS             19949..22420
                     /gene="gyrA"
                     /locus_tag="J8V14_001076"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBA0921195.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22656..22955
                     /locus_tag="J8V14_001077"
     CDS             22656..22955
                     /locus_tag="J8V14_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBA0921196.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            23005..23517
                     /gene="ssb"
                     /locus_tag="J8V14_001078"
     CDS             23005..23517
                     /gene="ssb"
                     /locus_tag="J8V14_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBA0921197.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23543..23779
                     /gene="rpsR"
                     /locus_tag="J8V14_001079"
     CDS             23543..23779
                     /gene="rpsR"
                     /locus_tag="J8V14_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBA0921198.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            23922..25895
                     /locus_tag="J8V14_001080"
     CDS             23922..25895
                     /locus_tag="J8V14_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBA0921199.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            25901..26353
                     /gene="rplI"
                     /locus_tag="J8V14_001081"
     CDS             25901..26353
                     /gene="rplI"
                     /locus_tag="J8V14_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBA0921200.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26627..27994
                     /gene="dnaB"
                     /locus_tag="J8V14_001082"
     CDS             26627..27994
                     /gene="dnaB"
                     /locus_tag="J8V14_001082"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBA0921201.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            28774..30066
                     /locus_tag="J8V14_001083"
     CDS             28774..30066
                     /locus_tag="J8V14_001083"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBA0921202.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9131 a         5977 c         5976 g         9189 t
ORIGIN      
        1 ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcttct
       61 tatttccgaa ggagttgctt ctacttccgc cgtttatacg tgtttagagc gtgagacaaa
      121 agtgttcttt acttttgtcc cacgctcttt aatgttttta agattcaact gaatagtttg
      181 gtgcttcttt tgtgatttgt acatcatgag ggtgtgattc ttttaatcca ctgccgctca
      241 tttggataaa ttgcgcttca tcacgtaatt gctgtagatt ggctgcccca acataaccca
      301 ttcctgaacg caaaccgccg atcatttgga aaataatatc tgcaacactt cctttataag
      361 caacccgacc ttcgattcct tctggtacta atttatttgc ttcattgaca ctaccttgga
      421 agtaacggtc ttttgaacct ttttccatcg cgcctaatga gcccatacct cggtatgttt
      481 tgaatcgacg tccttggaag atttcgaatt cacctggtga ttcatctgtc cctgccaaca
      541 tacttcctag catcactgca tgtccgccgg cagctaatgc tttgacgatg tcaccagaat
      601 atttgatccc tccgtctgca ataatcgctt tgccatattg acgagcaact gatgctgcat
      661 cataaatagc tgtgagctga ggtacaccta caccggctac cacacgtgtc gtacaaatcg
      721 atcctggtcc gataccaact tttacgacat ctacacctgc atcatataaa gcttttgtcg
      781 cttcagccgt cgcaacgttt cctgcaatca atgttgcatc tgcaaaagtt gaacggattt
      841 cttgaatttt tcggatcaca cctgcactat gtccatgagc tgtatcgatg atgattgcat
      901 ctgcccctgc ttctaataaa gcattcgcac gctcaaatgt atcgcttgtt acaccaacgg
      961 ctgctgctac aagcaatcga ccatgttcat cttttgctgc atttggaaat tcgatcactt
     1021 tctcgatgtc tttgatcgtg atcaatccgc ttaaacgacc ttcgttatct acgattggta
     1081 atttttcgat tttatgtttt tgcaaaatct tttctgcatc ttttagagag gtacctactg
     1141 gggcagtcac taagttgtct tttgtcatca catcagcaat cggcatgtga taatctgtta
     1201 caaaacgcat gtcgcggttt gtaattatcc ctaccaattt tctgttttca agtgtttcaa
     1261 cgattggcac accactgata cgatatttgc tcatcaattc ttctgcgtct gctacaagat
     1321 gctgcggcgt taagaagaac ggatcaatga ttacgccact ttcagaacgt ttgacttttc
     1381 ttacttcatc cgcttgctgg ctgatcgtca tgtttttatg gatcacacct agtccgcctt
     1441 gtcttgccat agcaatggcc atcttgctgt cggtaacagt atccatactt gcactgatga
     1501 ttggaatatt caacttaata tttttagcta attgtacgct catatccact tcgtttggta
     1561 acacgtgact ttctgctggt atcaataaga catcatcaaa agtaaatcct tttttcgtga
     1621 actttgtttc ccagttggac atttttagta ccactcctct aatttagttt atctattaaa
     1681 ataattgatt ttttcattta agtcaatgat ctacttggaa tcaagttagc tttctcatag
     1741 aataccaata taaagcgttt acatctgtgt gtattaaaag agattgtaaa aaagctgtcc
     1801 tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg aattggttgg
     1861 gtttattacc gcagatgcaa acttttgaac accgtttagt ggagaggctg tgaaaaagct
     1921 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
     1981 ttggacttaa taccgagaaa ctggcttttg aacaccgttt atcgtaaaaa gggactgtga
     2041 caagactttt gtcacagtcc cttactatta tttaactatg gataaacaaa atgaggttct
     2101 ttactacccc tagatatcta gcaaaatagc tgatcttttt gctagtttat cttcttggga
     2161 cggataaact tccaataatc ccgtattttt tcttcaagta gtaacggatt gctaaagcag
     2221 ttgccccgat aatgataact actactggat ctaatactgg attgataact ggtggcaata
     2281 gtgttgtcgc agaaaacagt acgacccaaa gaagtgtcgt tccacctagg ataagcaatg
     2341 tcttacccat tcctggtttc ttgcttcgat ctgctccggg atattcatat tgatagatgt
     2401 atttgtacat catataaaag acccagccac cgattgcaga acctagaacg agtgtagaaa
     2461 agccataagc tggcgttgtt ccacgtgaaa acaatccggt aatcgcaatc atgatactta
     2521 atagaccaag caacagcaac gtattatcta accacatcaa taaaggggtg gatgaatttt
     2581 ttacttctgc tggcttattg atgattgctt ccgttctttc agagactgtc ccaaacaatt
     2641 gacgggctgt tcttccgcct ttttgttctg caactaaagt aggcagaatg tcatgaaggg
     2701 cttttacttt ttcttcttca gacaaattcg ctgcttctaa agattttttc aaatcaaaaa
     2761 tgtactgttg atttcttttg gttaattttg tttctaaact attgttttct tttacgattg
     2821 ctcgtaattg ttctggttcc attatctaat agtcctcctt agccgttcct tttctcaacg
     2881 gtcgtcacaa aagacattct accatattct acagctagtt tgatagccct tttacacatt
     2941 aaagcggaat aacattacgt cgccgtcttg tacgacataa tcttttcctt ctaatcgaac
     3001 acgtccagct tcttttgctg catgcatgtt tccgtaatga tccaagtctt caaaagagac
     3061 agtttctgca cggataaatc cgcgttcgaa atctgaatgg atgattcctg ctgcttgtgg
     3121 tgctttgatt ccttttttga atgtccaagc acgtacttct tgttccccag cagtaaagta
     3181 agtagctaag cctaacaaat cataagcagc acggatcaac tgatccaaac ctgattcttc
     3241 gattcctaaa gcttctaaaa attccgcttt gtcttcttca tctaattctg cgatttcttc
     3301 ttctgcacga gcactgacca cgatcacttc tgcattttct tctgcagcaa agttgcgcac
     3361 ttcttgtaca taatgattcg cgtctgcatc tgctacgtca tcttctgaga cattggctac
     3421 ataaagaatt ggttttgtcg tcaataaaaa tagtgattta acgatttttt gttcttcttc
     3481 agtaaattcg atcgttcggg cagattttcc ttcttccagt acaggtttga ttttatccag
     3541 tacagctagt tctgccactg catctttatc tttggtttta gcaacttttg ctacacgagt
     3601 atagcgtttg ttgatagatt ctaagtctgc taacactagt tccaagttga tcgtatcaat
     3661 atcttctagt ggatcaacac gtccttctac gtgagtaata ttatcatcat caaaacagcg
     3721 aacaacgtga caaatagcgt ctacttggcg gatatggctt aagaattgat ttcccaaacc
     3781 ttctccttta cttgcgcctt ttacgattcc agcaatatct gtaaattcaa aagtcgtcgg
     3841 aacagttttt ttcggatgta caagttctgt taagcgttgc aaacgccagt caggtacttc
     3901 taccatccct acgttggggt cgatcgtcgc aaagggatag tttgctgctt ctgctcctgc
     3961 ttttgtgatc gcattaaaca aagttgattt tcctacgttg ggcaacccaa caattccagc
     4021 agtcaatgcc ataaatttat tcactctttc tctgcatttt tttcaattac tttttttaat
     4081 ttcttttcaa aatctcgacg ggtcatcatg acgatatgtc cgcaattcat acatctgatt
     4141 ttgatatctg cgcccattcg aatgacttcc cagcgattcg tctggcaagc gtgaggtttt
     4201 ttcatttcaa cgatatcgtg caagtcgtac ataaaaatag cctccttttt gtttattctt
     4261 catcaaattg aatattcaag atatcaagaa ttcgagtcaa atctttttga gaaagatatt
     4321 caatctcgat cttacctttc ccattttttt cttggatagc tacacttgta ccaaatttat
     4381 ccatcaaacg atcttcgctt tctcggatat aatatggttt ttcctttgct agccttggga
     4441 tttttttctt ttcttttcca tcattttgat ttaactcaga aacaagctgt tccagttggc
     4501 ggacagttaa attttcagtt actgcacgat tggctaattt caagatcaat gttttatttt
     4561 ttaaacctaa cagcgtacgg gcttgtccca tagataaacg ctgatcttgt accatttcct
     4621 tgacaagatc cggtaaagaa agcaagcgca aataattggc gatataaggt ctgctctttc
     4681 ctaaacgact agctacttct gcttgtgtta gtttcaggtt tttcatcagc atctcatatg
     4741 cttctgcttc ttctaacgga ttcagatctt ctcgctgcaa gttttccagc actgctactt
     4801 gcatcatcgc ttcttcgtca aagtctctaa cgatagcagg gatcgtttct ttttcagcta
     4861 gtttggacgc tcgaaaacgt cgctcccctg caataatctc atatcctttt actgcggaac
     4921 ggcgtacgat gatgggttga aagacacctg attgttcaat agaattagct agttcctgca
     4981 aagatacttc ttcaaatgtt ttacggggct gatacggatt tggtcgcagc tcattcaatg
     5041 gtagctggac aacggtatca tcttttacat ccaccgtctc taagttttct aaatcttgaa
     5101 acagtgcatc gattcctcgg cctaatcctt tacttttctt cacgacttac cacttccttt
     5161 gccaatgctt ggtagacttc agctcctcga gaacgtggat cgtagtcgat gatcggtttt
     5221 ccatgactag gtgcttctga taaacgaata tttcttggaa tgatcgtttc gtaaactttt
     5281 tcgcggaagt atttacgtac ttcttctacg acttcatttc ccagatttgt tcgtgcatca
     5341 tacattgtca gtaagactcc ctcgatctct aattctggat taaagtgttt ttggactaaa
     5401 cgaaccgtat tcagtaattg gcttaatcct tccaatgcgt aatactcaca ctgaacaggt
     5461 atcaaaatcg aatcacttgc tgtaaatgaa ttgattgtca gatggccaag agaaggcggg
     5521 caatcgatca aaatataatc atactgactg cttacttctg ctaaagatcc ttttaatctt
     5581 gattctcttg ccatcattga cgtcaattcg atctctgctc ctgctaattg gagagtcgca
     5641 ggcacaatac tcaaattttc gtgttcagtg atcaaagttg cctcatcaat tggcaattca
     5701 ttgactagaa catcatagat gtctcttgtc acatcaggtt ttctgatccc gacaccactg
     5761 gtagcatttc cttgtgcatc catatccact agcaatactt ttttgccaag gctagcaaga
     5821 caagcaccta gattcaccgt cgtcgttgtt ttgccgacgc cacctttttg gttcgcaaca
     5881 gatattattt gtgccatctc ttctcctcct atttgattgg ctgcttatta ggcagtccgg
     5941 gctttcttgg gtatttcttt ggggttggtt tctttttttg aataacgatg atatgccgtt
     6001 gatcagagac attcggcaag gtaaccgcct cttctttgag gaatttacca cctaataaag
     6061 caatcgcttg ttttgcttct gtcaattcct cttcactttt tgctgctttc aatgcataaa
     6121 aatagccttc ttgtttagcc aagggcaaac acaattccgc taacacattc aaacgagcaa
     6181 ccgcacgagc agtgacaagg tcaaatgatg cacgaaagtg aggattttgc ccaaatgttt
     6241 ctgcccggtc atgatataag gaagcatcga ttcctaataa tcgggttaac tcagataaga
     6301 aggttattcg tttattcaga gaatcaacga tcgttatctg cagttctgga aaggcaattt
     6361 ttaaaggtat gctaggaaac cctgcccccg aaccaacatc gcataaagat aaaggttgcg
     6421 taaagtctac ttcaaatgct aatgtaagtg aatcgtaaaa atgcttcaga tatacgtctt
     6481 tttcatccgt aatagctgta aggttcatct tttcgttcca ttcgactaat gtccgataat
     6541 aaatagcgaa ttgattcatt tgatgatcgg taagctctat ccctttttct gccaaggatt
     6601 ggcgaaattc ttctggtatc ataaatattc ctttcattgt gaaacagcaa ttgtttcaca
     6661 gattctctcc ctactttaac gcaaaatctc tagacatgca atccttccat atggattctt
     6721 aaaaatttga aaagtaagaa aagccgatag actttttctt tctagtatga ctcataaaat
     6781 atacacagaa gagagaacta gctttgagca atttctgcaa aaactagttc tctcttctat
     6841 ttattattac gtattacgct ggttcactga ctttcgcaat cttcccttgt tcgatataga
     6901 ccattaggat acttaggtct gcagggttta cgccactgat tcggctagct tgggcaattg
     6961 tttctggttg gattttctgc aatttttgtt ttgcttctgt tgctaatccg ttgattgctg
     7021 cgtagtcgat gttttctgga atctttttcg cttccatgcg ttttaatttt tctacttttt
     7081 ccatggcttt tttgatgtag ccttcatatt tgatttggat ttctacttgt tcgatttctt
     7141 ttcgtgtcaa ttcttcattt tctggaataa accgtagaag ttctgcataa gtaatctcag
     7201 gacgtcttaa aaagtcgctt gctagtacac catcttttaa tggtgccgca ttaatcgttg
     7261 ctaaaaagtc ttggacttct tctgttggtt tgattcgtac attcatcaaa cgtttgattt
     7321 cttgttcaac tgcttctttt ttcttcaaat aattcttgta ttgatcttct tttactaagc
     7381 ctacacggtg acccatttct gttaatcgca aatctgcatt atcatgacgt aaaagcagac
     7441 ggtattcagc acgtgaagtc agcaaacgat aaggttcgtt cgttcctttt gttaccaaat
     7501 catcgatcat gacaccgatg taaccgtcac ttcgtttgat cactaaaggt tcttttccct
     7561 gaactttcaa agctgcattg ataccggcaa tcagcccttg tccagccgct tcttcataac
     7621 cgcttgtacc atttgtttgt ccagctgtat aaagattttc aacgacttta gtttctaatg
     7681 tcggacggag ctggtgtggc tctactacgt catattcgat agcatatcct gtacgcatca
     7741 tttctacttt ttctaatcca gcaatagaat gaagcatgtt gacttgaaca tcctctggta
     7801 aagaagtaga taaaccttga acatagactt cttccgtatt taagccttct ggttccaaaa
     7861 ataattgatg acgtggtttg tctgcaaaac gcacgatctt atcttcgatg gatggacaat
     7921 agcgagcgcc taccccttca acgatccctg taaacattgg ggcacgatgt aagttgtctc
     7981 gaataatttg gtgagtcgtc tcacctgtat aggtcagcca gcaagattcc tgatctagtt
     8041 tataggcact atctggtgtg ctgaagctaa aatgattcgg ctctttgtca ccaggctgct
     8101 cttctgtaac agaatagtcg atcgtacttg attttactcg aggcggtgtt cctgttttga
     8161 accgttcgat agcaaagcca agttctttta gatgattcgc tagtccaata gaaggttgag
     8221 agttattcgg acctgatgaa tatttcaatt caccgataat gatttctccg cgaagtgctg
     8281 ttccggctgt aataacaact gctttggccc ggtagctcgc tcctgtggac gtcactactc
     8341 catgacaaac accatcttcg acgatcagtt cttcgacgat tccttgtctt agcgtcaaat
     8401 ttggttcttt ttcgatcgta tgtttcattt ctgttgaata agcatgtttg tctgcttgtg
     8461 ctcgcaatgc tcggacagct gggccttttc ctgtgttcaa catacgcatt tggatatagg
     8521 ttttatcgat attcttaccc atttctccgc ctaacgcatc gatttctcga acgacaacgc
     8581 ctttagctgg tccgcctaca gaaggattac atggcatgaa tgctaccatg tccaaattta
     8641 ttgtcagtaa taatgttttt gatcccatac gtgcagcagc aagtgcagct tctgaaccgg
     8701 cgtgtcctgc gccaacaacg atcacgtcat acgcatctgc ttcataatgt tccaatcgtt
     8761 tgttcctcct tttattttcc taaacaaaat tgactgaata attgagtgat tagctcatct
     8821 tgtacactgt cgccgacaac ttctcctaga tagtcccagc aacgcgtcat atctatttga
     8881 acgagatcca caggcattcc tgaatcaata cctgcaatga cttcttcaag tgagagggaa
     8941 gctttttcta acaacgcgat atggcgggta ttagaaacat aagtagcgtc tctttctcct
     9001 gtttctccgc tgaagaataa atcagaaata gctgattcca aacgatctag tccgtcgttt
     9061 ttagctacgg aaattgaaaa aactggttca ttttcgatca atttcttcaa tttttcttgt
     9121 tccagctgtg ctggcaaatc tgttttattt agaagaatga tccgtttcaa tccacttgtc
     9181 gcttccaata attgttcatc ttctgctgtc agtggttcgc tttggtttag aacaagtaag
     9241 atcaagtctg cttctgctaa tgcttttcgg cttctttcga ccccgatccg ttccaccaca
     9301 tcttctgttt cccgaattcc agctgtatcg attagcttga gcggtacccc ccgaacattg
     9361 acatactctt cgattacatc tcgagtcgtt cctgcaatat ccgtaacgat tgctttttct
     9421 tctcttaaca aatggtttaa caaacttgat ttgccgacat ttggccggcc gatgattgcc
     9481 gtacttaacc cttcccgcag aaccttccct tgcttggatg tagcaagtag cgcttggatt
     9541 ctttgttgga tcatctgcgc tttttcaagc aataacttcg tagtcaattc ttcgacatca
     9601 tcatattctg gataatcaat atttacttct acttgtgcta atgtctctaa aatctcttgt
     9661 ctcaatgagc ggatcaaagc agacagattg ccgtctaact gattaagagc tagtcccatt
     9721 gctttgtctg tttttgcccg aatcaaatcc atcacagctt ctgcctgtga aaggtcaaca
     9781 cgaccattca agaatgctcg ttttgtgaat tcgcctggct cagctaatct tgcaccttct
     9841 cttaacaata gctgcaaaat ttgattgact acaacgatcc caccgtgaca attgatttct
     9901 actacatctt cccgcgtaaa tgtctttggt gctctcatca cggagaccat gacttcatcg
     9961 acaagctgat tgctcttggg atcaacgata tgtccataat ggatcgtatg acttggtact
    10021 tctgataatc gtttgtttcc agattgataa actttatctg ctagttgcac agcttgatcg
    10081 ccacttaaac gaacgatact gattgcacct tctccaggtg gggtagaaat tgccgcaatc
    10141 gtatcaaatt ctaaagtaat ttcagccatt ttttcacttc ctttttcatt ttgcgcacaa
    10201 aaaaagtgcc cactccaccc tttatgcaca ataaacggtg aacttcgcac tttgactgct
    10261 tgttcttgat taaattctgg tcatagatta tcacacttgc tataaggaat caagtgttta
    10321 aaacaattag atgaagttga gcctgttctg cttccattac cttatattta attgtccttg
    10381 gatgtctctt tgcgttcttt ggtttattcg aagagaagat actgtataaa caaggcttta
    10441 tctttcttaa aaaaaacggt acactaaatg gtagcgctat ctaaactata cacttttgaa
    10501 aggagaaaat atgtgagccc gttcagatct gctgcattgg aagaagaaag tgaccctcgg
    10561 atcaacgatg tcgttttatc tacagggaat atcaaccaga aatatatcgt ccgcgatatc
    10621 gtttttgttg tggaatgctt agaagctgat ttgtttgacc catcttttcc caaagatgac
    10681 cttttcttaa caacgaagag gaaattaaag acgaaggtac ttgaatatgg tgcgaatgca
    10741 gtaatcaact gtcattttga gtatcagcaa aaaaaacgcg acggaaagta ttttctggaa
    10801 ctttttgctt atggaacagt gatccaattt atccaaacaa cgattggtta aatgaaacag
    10861 acaaaaaaag cggatcaaaa ctgaagacag tttcgatccg cttttttcta tcaaagttag
    10921 tgactacttc atcgtatact ctgataagaa tgatttctag taaaatatac ttggtttgaa
    10981 aaactttgtc aacgtgattt attcaaaaag taatggaatc aataatattt tttagcaggt
    11041 tctactacta aataacgata aggttcctca ccttccgaat gtgtttttac atgttcatct
    11101 ttgcttaatg cagcatggat ttgtttacgt tcaaatgcag gcattggttc taagaaaaca
    11161 ggctgccctg tccgttttac tttttcagcc gtgcgatcag ctaaacgctg aatgatctct
    11221 tggcgctttt cacggtaatt gcctacattg actactacag acagcttatt tcttgctaaa
    11281 cgatggatga agacttgtgc cagatattgc aatgcattca gtgtttttcc atgtttccca
    11341 atcagtatgc cttgcttttg tgtttctaaa tggaaaacaa ccatattatt tgattctctt
    11401 tccattttga caagagctgg agcatcaagc tgttttgaaa tttcagtaag ataaactgcc
    11461 aattccgtta aagctgcttc atcacctagc tcacttactt cttctgtttt ttcttcaaga
    11521 atcgtttcgt tgactacctc gatctcagct gttggttctt cttccaaaat ttcttctaca
    11581 gtctgttcta ctgtttcaga aactttttcg ctgatacttg gctggatcga aagccgtgca
    11641 ttctttcgac ccattcctag gaaacctttt tttccttctt ccagtatttc gatttcgact
    11701 tggtctttgg ccaaacccaa atcagccaga cctagttgga cagcttcgtc tattgttgca
    11761 gcttcataaa tcggcattag cattacctcc cctattattt ccgttttttc ttaggattca
    11821 ttgctttctt aagagcacgt tctctttcgc ggattctccg tgcttcttct tcacgctctt
    11881 ttcgaatttt gaaagggttg ttgatcagca gcgtttgtac aacttggaag gcattggaaa
    11941 ctacccagta taatgaaaga ccgctggcta aattgacacc catcactaaa atcattacag
    12001 gcatcacgaa attcatgatc ttcattgatg cattcgattc aatttgactc atgctagata
    12061 aatacgtact tgcaaaagta aagattgctg ctaaaatcgg caaaataaat gttggatccg
    12121 cgttccctaa gttcaaccat aagaaatgcc cttgtttcaa ctctggtaca cgtgaaatag
    12181 attgccataa cgccatcaag attggcattt gtactaataa aggaagacaa ccagcatatg
    12241 gattgacgcc attttctgca tataaacgct gctgctcttc tcttaactta ctttgtgttt
    12301 ccggatctct tgaactatat tgttgctgca gtgcttttaa tttcggttgt aattcttgcg
    12361 ttttacgcat gctctttgtt tggaaatgca tcaacggcaa taggatcacg cggatgatca
    12421 gtgtaaataa aatgatccct atccctgcat taccaaatga caacgccttg atcgcctcag
    12481 caaagtaata aacaatataa cgatcccaaa ttcctgtact ctgtgcgctg acttccccag
    12541 ttccgcaacc ggataagacg atgaccaaac taacgatacc ggccatcaaa agtaaacgtt
    12601 tgtatttctt cacttactct tttcctctct gtcgattatt tttgcaagtt ttaagacgtg
    12661 gactaaattg gagcgaacct catcataagt caaccccttc acacttggtc tagcaatcac
    12721 aataaaatcc aactccggat caatttcttc ttttatagaa taaactgctg ctcggatttt
    12781 tcgtttgact tcattgcggt ttaccgcgtt gccgaccttt tttcctacag ataaaccaac
    12841 tcgaaaatga ggctgtcccg gtttttccaa acggtaaacg acgaatttgc ggttagcaaa
    12901 agaatctccc gtatggaaca cggcttgaaa ctctcgttct ttttttactc gatacgcttt
    12961 tctcattagt tttctttcct tttctatctc tcacttacgc tcataatcat accataatta
    13021 cgattgtcct ttcatcattt ccctaaaaaa gagtataaaa aaaaccactg aaaccccagt
    13081 ggcctaagct gaaagaactt ttcttccttt gcgacgacgg ctagctaaca cacgacgtcc
    13141 atttttagta ctcatacgtt tacggaatcc gtgaactttt tgacgtttac gtttatttgg
    13201 ttggtatgtt cttttcatat attccacctc caaaatgttc ttatctattt tataaacaca
    13261 gacatacttt gatagtataa cgacaaagta gtgattttgt caatataaaa tagaagatcg
    13321 attgttaaaa cttatccaca agattatttt tttctatggg agtcctatgt ggaaaaagtt
    13381 atgcacaggg attatccaca gataataaat ggctggtttt acacagaaaa cacacgtgta
    13441 aaaaaagtaa tccacaatca ggtgatttcc tgtgtattcc gtctcctaac tattggtata
    13501 tctatatttt ttatccacag atgcaagagg aaatctattg atttttacga gttttccacc
    13561 tgtgcattag tggtgtggaa aacttttttt tgacaactgt gtttttctct tttttctttt
    13621 tgtcgattgt gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact
    13681 attgaaaaaa atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta
    13741 tatggtatcc ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc
    13801 gcctgctagc tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca
    13861 gctcgttgtt gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac
    13921 aaaaatcgtt gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac
    13981 tggcgaagaa gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc
    14041 tagtccgcta aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg
    14101 taaaggaaac cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat
    14161 ttataacccg ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc
    14221 aattggtcat caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga
    14281 aacttttgct aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca
    14341 agaatatcga aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga
    14401 agctacgcaa gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat
    14461 cgtactaacc agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gcctcgtttc
    14521 tcgctttgca tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc
    14581 gattttacgt aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta
    14641 catcgcagga caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca
    14701 agcttttgct gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa
    14761 aacgttaaaa tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt
    14821 ggcaaagtat tatcacatcc aattgaaaga tatcaaagga aagaaacgcg tcaaatctat
    14881 tgttgttcct agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc
    14941 gaaaatcgga gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa
    15001 aatccagcag ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa
    15061 ctttttacta aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa
    15121 aaaacgttca aaagttatgc acactttttc cacagctgtt ttttcgtttc tctcttacgt
    15181 tttttgagtt ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat
    15241 aataaataaa caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc
    15301 tttatgcagg aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt
    15361 ttgacaggtg taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct
    15421 gatatatcaa ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa
    15481 tctactggtt ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct
    15541 gaagaaacat ttactttaga agttttagaa aataaacaag tagcgattac ttctggaaaa
    15601 gcgaatttta tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa
    15661 agccataacc agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt
    15721 tttgctgtct cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct
    15781 gataattctt tattggctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca
    15841 gtagaacaag cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta
    15901 tctcgctcat taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg
    15961 ttgttcaaaa cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatcctgat
    16021 accaatcgtt tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt
    16081 ttagcggcga tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt
    16141 ttatctattc gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta
    16201 gaagaaagct tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg
    16261 gattatatga aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc
    16321 tctgctattc gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg
    16381 attactcctg tacgtacaaa ttaacaatga ttttcattgt tcttgaaaat ttaattgaaa
    16441 agaaatagaa aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg
    16501 tttatttttg tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg
    16561 ataaatttgt tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat
    16621 gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa
    16681 atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa
    16741 aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc
    16801 atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat
    16861 aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga
    16921 attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt
    16981 ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt
    17041 gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac
    17101 tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga
    17161 acaaaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga
    17221 tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg
    17281 ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag
    17341 aaaccagtat ttaaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat
    17401 tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt
    17461 caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca
    17521 gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat
    17581 ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac
    17641 aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca
    17701 gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat
    17761 cgatttgatt aaagaagaga cgggggaata tccaatcctt ttgttagatg atgtgatgag
    17821 cgagctggat gatagtcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac
    17881 gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat
    17941 tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt
    18001 ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa
    18061 gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac
    18121 cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa
    18181 atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata
    18241 cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta
    18301 catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta
    18361 ggatcttctg ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga
    18421 aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa
    18481 caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag
    18541 acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac
    18601 cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac
    18661 cattatgacg gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt
    18721 tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg
    18781 caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat
    18841 gaaggcggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat
    18901 gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga
    18961 gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca
    19021 aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat
    19081 ttcatgaaat tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta
    19141 ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt
    19201 gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa
    19261 aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt
    19321 aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca
    19381 agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca
    19441 gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact
    19501 gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac
    19561 atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta
    19621 aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa
    19681 caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt
    19741 ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg
    19801 gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg
    19861 gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta
    19921 gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga
    19981 tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt
    20041 cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata
    20101 cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt
    20161 cggagatgtc atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt
    20221 acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg
    20281 ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat
    20341 cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga
    20401 tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg
    20461 aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt
    20521 agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga
    20581 agtattacct ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg
    20641 aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga
    20701 gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc
    20761 aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga
    20821 tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc
    20881 cagcgcatct gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg
    20941 attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct
    21001 tgaaaactat atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga
    21061 aaaagcagaa gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga
    21121 tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat
    21181 cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg
    21241 attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat
    21301 tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga
    21361 attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga
    21421 ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac
    21481 aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg
    21541 acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg
    21601 ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg
    21661 atacgaaatt ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg
    21721 tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg
    21781 ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt
    21841 tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt
    21901 caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc
    21961 tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg
    22021 aatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga
    22081 agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt
    22141 gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact
    22201 agctggattg accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt
    22261 cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg
    22321 tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc
    22381 tgcagaacaa acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta
    22441 agctagcaag aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt
    22501 gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt
    22561 gtataatcag agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac
    22621 ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca
    22681 tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg
    22741 atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc
    22801 gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt
    22861 ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata
    22921 tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga
    22981 aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg
    23041 aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct
    23101 gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg
    23161 atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt
    23221 gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg
    23281 acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc
    23341 caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat
    23401 aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct
    23461 gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg
    23521 aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa
    23581 agtagactac attgctgcta accatattga atatatcgat tataaagata tcgaattact
    23641 aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc
    23701 taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc
    23761 tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta
    23821 ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa
    23881 gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc
    23941 atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct
    24001 taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg
    24061 ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc
    24121 aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag
    24181 tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa
    24241 tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag
    24301 aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag
    24361 taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga
    24421 cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata
    24481 aattggatga caaagagatt tcttatttga actctttcgt cactacacta gtttctgact
    24541 ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag
    24601 cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc
    24661 gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct
    24721 caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc
    24781 gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg
    24841 gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg
    24901 ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg
    24961 atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa
    25021 aagcttacat cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag
    25081 aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc
    25141 tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct
    25201 ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg
    25261 aagagtttcc aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag
    25321 agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg
    25381 aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa
    25441 ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag
    25501 tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg
    25561 gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata
    25621 atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag
    25681 ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca
    25741 cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag
    25801 ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca
    25861 aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca
    25921 agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca
    25981 aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt
    26041 agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa
    26101 gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga
    26161 agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca
    26221 atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg
    26281 ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt
    26341 cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa
    26401 aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa
    26461 tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg
    26521 agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc
    26581 tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg
    26641 gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt
    26701 tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg
    26761 tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat
    26821 tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg
    26881 tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta
    26941 tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat
    27001 cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg
    27061 cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt
    27121 actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg
    27181 tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact
    27241 aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca
    27301 aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga
    27361 atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac
    27421 aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg
    27481 agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg
    27541 tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt
    27601 gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact
    27661 gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg
    27721 agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat
    27781 tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg
    27841 cggagatgac gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga
    27901 gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa
    27961 gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat
    28021 cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat
    28081 ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata
    28141 aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac
    28201 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc
    28261 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag
    28321 aagcagtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct
    28381 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata
    28441 tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
    28501 ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa
    28561 atttgagaag caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac
    28621 aacctctttt ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa
    28681 aataaaaata aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt
    28741 tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg
    28801 caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg
    28861 attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact
    28921 tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga
    28981 aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa
    29041 ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat
    29101 atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt
    29161 aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac
    29221 ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt
    29281 caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat
    29341 tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat
    29401 gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc
    29461 gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc
    29521 ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg
    29581 tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc
    29641 cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt
    29701 gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac
    29761 tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat
    29821 ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc
    29881 tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg
    29941 ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca
    30001 acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa
    30061 atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt
    30121 tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg
    30181 ataggctgtc tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    30241 cagtcccttt ttccgaacaa acggtgttca aaa
//
LOCUS       DADQRX010000026        28788 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.026, whole genome shotgun sequence.
ACCESSION   DADQRX010000026 DADQRX010000000
VERSION     DADQRX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.026"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1212
                     /locus_tag="J8V14_001084"
     CDS             <1..1212
                     /locus_tag="J8V14_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBA0921203.1"
                     /translation="VLGFDPAYRTGCKLAVVDATGKVLAIEVIYPHKPAAQAKREAAG
                     PAFIQLINKYQVDMVAIGNGTASRESELFVAEQLKSADHKTYYAIVNEAGASVYSASE
                     IARKEFPHLQVEERSAVSIARRLQDPLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDF
                     VVETAVNQVGVDVNTASPQLLQHISGLNKTTAQNIVIYREENGEFTARTQLKKVPRLG
                     PKAYEQAIGFLRVPGGKNILDNTGIHPESYSIAKEILTSVQLSEKELGTEEAVEKLTR
                     LSVEKLAESLSVGEETLADILAGLTQPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTG
                     TVRNVIDFGAFVDIGVKQDGLVHISKLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGR
                     ISLTMLSPYEE"
     gene            1232..1714
                     /locus_tag="J8V14_001085"
     CDS             1232..1714
                     /locus_tag="J8V14_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBA0921204.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1730..2491
                     /locus_tag="J8V14_001086"
     CDS             1730..2491
                     /locus_tag="J8V14_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBA0921205.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2622..3956
                     /locus_tag="J8V14_001087"
     CDS             2622..3956
                     /locus_tag="J8V14_001087"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBA0921206.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            4263..4748
                     /locus_tag="J8V14_001088"
     CDS             4263..4748
                     /locus_tag="J8V14_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBA0921207.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            4741..5424
                     /locus_tag="J8V14_001089"
     CDS             4741..5424
                     /locus_tag="J8V14_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBA0921208.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            5550..8195
                     /gene="polA"
                     /locus_tag="J8V14_001090"
     CDS             5550..8195
                     /gene="polA"
                     /locus_tag="J8V14_001090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBA0921209.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            8240..9076
                     /gene="mutM"
                     /locus_tag="J8V14_001091"
     CDS             8240..9076
                     /gene="mutM"
                     /locus_tag="J8V14_001091"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBA0921210.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            9040..9669
                     /locus_tag="J8V14_001092"
     CDS             9040..9669
                     /locus_tag="J8V14_001092"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBA0921211.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            9721..10053
                     /locus_tag="J8V14_001093"
     CDS             9721..10053
                     /locus_tag="J8V14_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBA0921212.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            10365..10856
                     /gene="nrdR"
                     /locus_tag="J8V14_001094"
     CDS             10365..10856
                     /gene="nrdR"
                     /locus_tag="J8V14_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBA0921213.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            10879..12252
                     /locus_tag="J8V14_001095"
     CDS             10879..12252
                     /locus_tag="J8V14_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HBA0921214.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            12252..13178
                     /gene="dnaI"
                     /locus_tag="J8V14_001096"
     CDS             12252..13178
                     /gene="dnaI"
                     /locus_tag="J8V14_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBA0921215.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            13307..13747
                     /locus_tag="J8V14_001097"
     CDS             13307..13747
                     /locus_tag="J8V14_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA0921216.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            13891..14592
                     /locus_tag="J8V14_001098"
     CDS             13891..14592
                     /locus_tag="J8V14_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBA0921217.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            14589..15710
                     /locus_tag="J8V14_001099"
     CDS             14589..15710
                     /locus_tag="J8V14_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBA0921218.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            15725..16957
                     /gene="pepT"
                     /locus_tag="J8V14_001100"
     CDS             15725..16957
                     /gene="pepT"
                     /locus_tag="J8V14_001100"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBA0921219.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            17089..17997
                     /locus_tag="J8V14_001101"
     CDS             17089..17997
                     /locus_tag="J8V14_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBA0921220.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            18032..18304
                     /locus_tag="J8V14_001102"
     CDS             18032..18304
                     /locus_tag="J8V14_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBA0921221.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            18315..19361
                     /locus_tag="J8V14_001103"
     CDS             18315..19361
                     /locus_tag="J8V14_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBA0921222.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            19612..22221
                     /gene="clpB"
                     /locus_tag="J8V14_001104"
     CDS             19612..22221
                     /gene="clpB"
                     /locus_tag="J8V14_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBA0921223.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(22372..23043)
                     /locus_tag="J8V14_001105"
     CDS             complement(22372..23043)
                     /locus_tag="J8V14_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921224.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(23036..23530)
                     /locus_tag="J8V14_001106"
     CDS             complement(23036..23530)
                     /locus_tag="J8V14_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBA0921225.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(23550..24770)
                     /locus_tag="J8V14_001107"
     CDS             complement(23550..24770)
                     /locus_tag="J8V14_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0921226.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            24917..26752
                     /gene="lepA"
                     /locus_tag="J8V14_001108"
     CDS             24917..26752
                     /gene="lepA"
                     /locus_tag="J8V14_001108"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBA0921227.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            26948..27859
                     /locus_tag="J8V14_001109"
     CDS             26948..27859
                     /locus_tag="J8V14_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921228.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         9743 a         5020 c         6144 g         7881 t
ORIGIN      
        1 gtcctgggat ttgatccggc ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca
       61 gggaaagttt tagcaatcga agttatttat cctcataaac ctgcagcgca agcaaaaaga
      121 gaagctgctg gaccagcttt tatccaatta attaataaat atcaagtaga tatggtagct
      181 atcggaaatg gtacagccag tcgggaatct gaactttttg tggctgagca gttgaaatcc
      241 gccgaccata aaacctatta cgctatcgtc aacgaagcag gcgcttctgt ttactccgct
      301 agcgaaattg cacgaaaaga atttccgcat ctacaagttg aagaaagaag tgctgtttct
      361 attgcccgtc gcctgcaaga tcctttagct gaattagtca aaatcgatcc taaagctgtc
      421 ggagtaggcc agtaccaaca tgacgtttct caaaaacgtt tggctgaaca attggatttt
      481 gttgtggaaa cagcggttaa ccaagtaggt gtggatgtga atacagcaag cccgcagctc
      541 ttgcagcata tatcaggatt gaataaaaca actgcccaaa acattgtcat ttatcgtgaa
      601 gaaaacggag aattcaccgc acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa
      661 gcatatgaac aagcaatcgg attcttgcga gtccctggtg ggaaaaacat tttggacaac
      721 acaggtatcc atcccgaaag ttattctatt gcaaaagaaa tattaacttc agtccaactt
      781 tcagaaaaag agcttggtac agaagaagca gtagaaaaat tgacacgatt atccgtagag
      841 aaactggctg aaagcctatc agtcggagaa gaaacgttag ccgatatttt agctgggctg
      901 acacaacctg gacgagatat gcgtgatgag atgccagcgc ccttattacg tacagatgta
      961 ttgagtatgg aagacttgaa accagggatg gaattgactg gaacggtccg taatgtcatt
     1021 gacttcggcg catttgttga tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa
     1081 ctcagcaaaa aattcgtcaa gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca
     1141 gtttggatag aacaagtcga tacgaaaaaa ggcagaatca gtttgacgat gctttcccct
     1201 tatgaagagt aacaatgagg agtaacaaaa attgacacaa gaagaactgc agcagttagt
     1261 agaagagata tcattaaaaa gcttccagct tcctttcaag caccaagcaa tgttcaatcc
     1321 acgcttacgt acaactggcg ggcgctatca tttaaacgat catcatttag attttaatgc
     1381 gaagctattt gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg
     1441 ccattatcat cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta
     1501 tcttttgaaa aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa
     1561 gatggagtat tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga
     1621 tgtacgcaaa tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag
     1681 agaagacttt aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag
     1741 tgagcagagg aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga
     1801 gggaaatttt gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat
     1861 cttagctgat aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt
     1921 cggaggggtg gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca
     1981 ttttgaagat ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga
     2041 tttgcccgat aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac
     2101 tttttcactg gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa
     2161 tgatccgaca ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca
     2221 aactattttc gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat
     2281 catctttaca gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt
     2341 attaggagaa atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga
     2401 ttttattcct cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga
     2461 gtcaataaaa acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca
     2521 aataaatttg gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa
     2581 tagctaaaag aatggacgct agataagaga agaggattca aatggaaaat caaaataaat
     2641 tgtatcattt tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg
     2701 aacaaggtct aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt
     2761 tagaaaaagc aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga
     2821 cgatcattgc aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat
     2881 taggtttgga agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga
     2941 gtatcgctgt aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat
     3001 tgagtggtgt ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc
     3061 aagcggaatt cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac
     3121 cagactatgc aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg
     3181 acgatgtatt tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt
     3241 atggtgacga tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg
     3301 tcacagaaaa cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt
     3361 ttgatgtcta tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac
     3421 acaatatctt aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga
     3481 aagaagttgc agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga
     3541 tcgtagcaga catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa
     3601 cgattgatgg cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc
     3661 atacgtttac gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg
     3721 ataaagttta cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga
     3781 ttgaagacct tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat
     3841 ctccattgtt agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa
     3901 aatttgaaca agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca
     3961 attactgcag tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc
     4021 cccttttttg caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
     4081 aatcaaatca aaaacatgag aagctgtttt ttcttggctg ttcttattat ttgatgcagg
     4141 acagcttttt cacgacctct gtaaactata ggaatctgtt aaatcaaggg agcagaactt
     4201 gccttttacg ctacttttgg tacaataatg tatgattaaa aataaactgg agggtttcta
     4261 tcttgaacat aggtaagccg agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg
     4321 actcgttatc cttaacagaa tctattgaag ataaccagct tttgctgtat ttgggattga
     4381 cgaatgatgc aaatcctttg tatatccaac atgactttgc acaaaaaaca gaatatcaac
     4441 agccaatcgt tccatcagtg atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc
     4501 ttcctggacc aggatcacat gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc
     4561 attatgaaac actgactttt cagtttgagg tcataaaagt agataagatg aaggatgtgg
     4621 tgacgatttc tgtagaagcg gagaatacga acgaagatcg tgtgttggat gctgtcgtta
     4681 tggtccaacc gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga
     4741 atgaataatc caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga
     4801 tttttaggta tgggacttgc ccttagtgca gtcacttctt acttggctct ttgggtattt
     4861 cctttgcaat tagcaacttt tatcaataat tttccactag gctttgtggg tctgtggatc
     4921 gttgaactga ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc gagcttagca
     4981 atcggtggat ttattgccta ctctcttttg aatggcataa cattagcggt tactcttgca
     5041 tactacgata ttggaacagt aacgcaagct ttcttgacag ctactttgat gttcatgggg
     5101 atgtctgttg tcggagtaac aacaaaaaga gatcttagcg gggttggaag agcaggacag
     5161 gcagctttaa tcggagtgat cattgcacta gtcttgaaca tcttcatttt gagaagtagc
     5221 gtagttgagc tgttcatctc cctactgtta gtcgtcatct ttgcagggat tacagcttat
     5281 gataaccaaa gcatccgtaa tatctatgtc cagacaaacg gaacagcaca tagtggaatt
     5341 gctgtattca tggcattgaa gctgtatttg gactttatca acttgttctt ggcgtttctg
     5401 agaatctttg gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa
     5461 ttatttggta tggtcccgct ttttcttttc gcattccgca ggattctttg ttagaatgaa
     5521 taagaacttt acagtaagga gttaagaaga tgacaaaaaa taaattatta ctcgttgatg
     5581 ggaacagtgt ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa
     5641 atagaaacgg tcttcataca aatgcaatct atgcattcaa tacgatgttt gaaaatgtta
     5701 tgcaaaaaga gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa acgacttttc
     5761 ggacagaaat gtatgcggag tacaaaggtg gaagatccaa aacacctggc gaattcaaag
     5821 aacaaatgcc atatatacgt gatttgttga caggactagg ggtacagtat tacgaattgc
     5881 ccaattatga agcagatgat attatcggta ctttggcaga aaaagtagac aaagatcaat
     5941 ttgatgtcgt cgtgctttca ggtgatcggg atttgactca attagctagt aaagaagtaa
     6001 aagtggatat cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca gaacatgtag
     6061 cggaaaaata tgatgggttg acgccaaaac agattattga catgaaggga ctagccggag
     6121 atgcctctga caatatcccc ggtgtgacta aaatcggtga aaaaacagct atcaagctat
     6181 tgaaacaata tggctcagtc gaaggaattt atgaacatat cgatgaaatg aagcaaagca
     6241 aaatgaaaga aaatctgata aatgacaaag agcaggcttt cttatcgaaa aaattggcga
     6301 ccatcaatac ttcagcgcct gtggatgtct ctatcgactc attgaagtat gaagggaaaa
     6361 atctcgataa actcgttccg ttttataaag aaatggattt caatcagttc ctttctaaat
     6421 tgaatatcgt agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat
     6481 tcaaagagga aatgtttact acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt
     6541 atcatacaga ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt tatgtcacga
     6601 acgatctatc tgtattcgaa aaccgagcgt tccattcttg gatcactgat ccgactcggc
     6661 tgaaaaaagt atatgatgcg aaacgaactt atgttgcttt aaaccgctat actggaaaaa
     6721 cgaaaggaat cgattttgat gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa
     6781 gcacagatat cgaaggaata gctgctcatt acggatacaa cgatatccaa tcagatgaag
     6841 ctgtatacgg caaaggggcg aaaaaaggtt taccagaaga agaggaactc ttttttgctc
     6901 atttagcgcg aaaagtagct gcaatcaatg cgttgacacc aaaattgagc caagaactgg
     6961 cagaaaaaaa ccaagaagat cttttccgga aaatggaact gccattgtct atcattttgg
     7021 cagagatgga aataagcggg atcaaagtag atgcgactcg cttacaagaa atgaagggcg
     7081 agttctctgc tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat
     7141 tcaatttgaa ttcaccaaag caattaggag tgatcttatt tgaaaaaatg ggattacccg
     7201 ttatcaaaaa aacaaaaact ggatattcca cagccgtaga tgtactggaa cagcttcgtg
     7261 aacaagcacc aatcgtagag gatatcctca cctatcgtca aattgcaaaa attcaatcta
     7321 cctatgtcga gggactgctg aaagtgatcc aatcagatgg aaaagtgcat acccgttatg
     7381 tccagacatt gacccaaaca ggacgattaa gctctgttga tcctaatttg cagaacatcc
     7441 cgattcgatt agatgaaggg agaaagatac gtcaggcatt tgttcctaga gaaaaagact
     7501 ggctgattta ttcttctgac tattcacaaa tcgagttacg cgtacttgcg catatctcaa
     7561 atgatgaaca tttgaaagct gccttcctag aaggtcaaga tatccatgcc agcacagcga
     7621 tgcgtgtatt tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa
     7681 aagcagtgaa ttttggtatc gtgtatggca tctctgatta cggactttca caaaatctag
     7741 gaatcagccg aaaagcagca cagcaataca tcgatacgta ctttgaaaaa tacccaggtg
     7801 tcaaagaata tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc
     7861 tgtatcaccg ccgtcgatat ctgccagaca tcaattcaag aaattacaat atccgttctt
     7921 ttgcggaacg aacagctatc aatacaccaa tccaaggaag tgctgctgat attttgaaaa
     7981 tcgcgatgat cgaattggat aaacgattaa aagaaacagg tcttcaagct acgatgctgt
     8041 tacaagttca tgatgaactt gtcttcgagg taccggaaaa agaacttgaa tcattagata
     8101 aattggtgaa ggaagttatg gaacaagctg tttctcttca tgtaccactg atcactgaca
     8161 gcagttgggg aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca gagcaatata
     8221 atgaaggtga aaagaaaaaa tgccagaatt accagaagta gaaaccgttc gtaaaggatt
     8281 ggaacggtta gtagtaggaa aaacgattca aaaagttcag gtcttgtggc ccaagatcat
     8341 cgagcaacca gagacaccca tctttgaggc ttctcttgtt ggcgaaacca tccaatccat
     8401 tggacgtaga gggaagtttt taatttttca tctcgatcat tgtgaactga tctcacactt
     8461 gcgaatggaa ggaaaatatc aatttacgaa agaaaataca ccaatcgata aacatacgca
     8521 tgtacttttc ttttttgagg atggaagcca acttcgctac aacgatgtac gaaaatttgg
     8581 tcgtatgaca atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca tgaaattggg
     8641 acctgagcct ttgccagata gcttcttatt agcagatttc gcaaatggac tgaaaaagtc
     8701 ccacaaagca atcaagccat tgctgctaga ccaacgtcta gtgactggtc taggtaatat
     8761 ttatgtagat gaagcattat gggaagcgaa gatccatcca gaacaaccgg cgaacacact
     8821 gcgccccaaa gaggtagaac aattacgtct atcgatcatt gatgttttgg atcgtgcaat
     8881 agaagccgga ggtaccacga ttcgcagtta tttgaatgca ttaggagaat caggcggatt
     8941 tcaagtagca ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc
     9001 gatcgtgaaa acaaaagtgg cacaaagggg gacgcactat tgtccgaatt gtcaaaggct
     9061 aaaaatcgca aagtaggatt tgttttaggt ttgacgggtg gaatagctac cggtaaaagt
     9121 acggcagcca aagtctttca gtctcatgga tttcctttga tcgatggcga tgtgattgcc
     9181 agagaaactg ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa
     9241 gaggtattgc agcctgatgg gcaactagat cgagggaaac ttgggatgat tgtttttcca
     9301 tcaaaggaaa aacggcaaaa acttgatcag ctattagatc cgtttatccg aaaagcgata
     9361 aaagatcaaa tcgcctctct aagttccaat catcctttag taattgtaga tatcccattg
     9421 ctctacgaag gtcattatga ccactatatg gatgcagtag ctgtcgttta tactacgcca
     9481 gaaactcagc ttcagcgttt gatgaaacgt aatcaactga cgctagaaca agcgcagcag
     9541 agaattagta gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac
     9601 aacaatggaa caaaagaaaa acttgtggga caaatcgaaa attggctttt tgctaatcgc
     9661 tttctttgag tatctgtgtc aattttgcta tcctgagtat atagaaagtg agggatagcg
     9721 atgggatcat taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc
     9781 gagatgctca ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa
     9841 gaaaaagaaa agcagtacca agcaagaatc gatgaaatag aagaccagca ggcagaagaa
     9901 gaagcgaagc ggcaagattt gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa
     9961 acaccgctcc cagaaagtga ccacattccc gatgagcaaa aaatcgagga acagcgaatg
    10021 aaacactata caggaatcga agagaagtta taaaatagta aaaaatcgag atgtgacttg
    10081 aagcagatga gaaggtgttc atctgcttca agttttttgt gctagatgac taaatcagct
    10141 tttgtgtaag ttatttctca gtaatcaagg tgatcagtat tggaaaattg aaatttagta
    10201 aggacagggg acacaattac gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt
    10261 tgggtaggac agtcgcgttt gtgatttgtt tcgtgttata atacaactag ttgtaaagaa
    10321 agacacatcc tttatataaa atgcgatgca agaggtgaga caaaatgaga tgtcccagat
    10381 gtcaacataa taattcacga gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc
    10441 gtcgccgtcg cgaatgtgag aattgttcgt tccgattcac tacatttgaa cgaattgaag
    10501 ctgctccgtt attagtaatc aagaaaaacg gggaacgcga agagttcaat cgcgataaaa
    10561 ttttaagagg actcatccgt tcagcagaaa aaagacctgt agctatggaa caaatggaac
    10621 aaatcgtcaa ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga
    10681 atctaattgg tgaatatgtg atggaagatt tagttgattt agacgaaatc gcttatattc
    10741 gttttgcaag tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa gagttgcaag
    10801 atatcgtgga taaagcaaaa gaaaataata aagagaataa taaagaaaac aattaagaaa
    10861 caaaaaggag gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac
    10921 caaggaagaa aagcggctcc gctgagagaa gacagcaaga acgccttgct gtatctttat
    10981 cagccactaa tcggcggaga agccttgagt atctatttca ccctgttatc agaaatctct
    11041 caagaatctg gcaaaggtcc tgaagggatg catgcagatc tgcttagtag tctaggctgc
    11101 ggtatcccgc aattttatga tgcaagaaaa aagctagaag gaatcggctt actggatgtt
    11161 tattttaaag aagatagtga tctgggacct cgttttattt atgagctgtt agaaccactc
    11221 attcctaaag catttttcaa agactctgtt ctggcttttc ttcttctgga aaaagttggt
    11281 gaacgacgat tccagaagct tacccaattc tttgaaccta aaaggcatac agcagaggga
    11341 tatcaaaaaa tcacaaaaaa atttttagat gtttatcgct tcaatgaagc agtgtacgct
    11401 gctaatcaag aaaacatcga atcgctgaat aaatcttttg aagaatctga atcagtagca
    11461 ttacttgaag cagattcgac attcgattgg cagtttttga atgattggct ggcaaaaaaa
    11521 catattgcaa gtcttgatga tccaatcatc aaacaagtcg acatgtatca tcagctttac
    11581 ggtttcgatg aattgacact tggcgaactg atcagtcaat cttatgactt tagtgaacaa
    11641 aaagtatctg aaaaagaact gcaaaaagcc attttggcaa tgcagagacg tccggtgaag
    11701 caaacaaaag aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat
    11761 ttgacaaacg cagcgcttca gctgataaaa gaagcacaga gcattccacc gatgaaatat
    11821 ctggaagcaa tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa ctggttactg
    11881 caagatcttg tttctcaatc aggtctttcc agtagcgtga tcaatgtttt gctgaattat
    11941 gtactagtga tcaagaataa tgctagctta aatgccagtt atgtaaatac agtggcaaat
    12001 gaatgggcac aaaagaaaat cgccacagca gaagaagcga tccagcacat tcggcaaata
    12061 agtaatcaag caaaacaatc aaaacaaaag aagcaaacaa attatcaaac tggcaagcga
    12121 aatgttcgac gggaaaagct tcctgattgg gtcaaccagc caaaagatga aaaacagatc
    12181 agcagcgaga aaaaagcaga gattgaccgc cgttttaaag aatacctggc aaaaaaggag
    12241 ggtgatatct agtggaagac gtaggcaaag aattgaagag gatcatcagc aaacgcaatt
    12301 atcaagaaaa gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat gttcaagctt
    12361 ttctaagcgc acaccaagaa gaattgacac aagcggatat tgaaaaaagt tacgctaaat
    12421 tgtatgaatt cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc
    12481 ccggctatga gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa
    12541 cggaagcatt gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg
    12601 atatgccaaa agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag
    12661 gatatgcgag cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt
    12721 atcataaagg actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag
    12781 caacagcgag agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat
    12841 ttgccgttga gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag
    12901 tgaaacgttc acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga
    12961 tacgagacga agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct
    13021 tcttctcatc gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg
    13081 gagatgaaga gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag
    13141 aaatcgaaat gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac
    13201 atttgaccta gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga
    13261 caatcaccgc aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt
    13321 ttctagtata tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag
    13381 caacacaggt tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa
    13441 aacggagaga caaagcatat ttgtcggaac gattgaaagt atcactcaag tagaagcaga
    13501 agaagaaacg cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat
    13561 gggaacgtca ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt
    13621 ggacataaat gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat
    13681 catgacgatg tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa
    13741 caaataacca ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt
    13801 atatttttta aggagatatg ggaatttttt tattcctagt ctccttattt tatttggaaa
    13861 atgctataat tatctagaag gagtgaagct atgaaccatt tagatttatc tcagcgctta
    13921 gccgcagtgg gtgactttgt ccctgaatcc gctcgtttag cagacattgg atcagatcac
    13981 gcttacttac ccgttgcatt gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa
    14041 gtcgttcaag gtccttatga atcagcacgt aagcaagtca aaaaagatgg attagaagac
    14101 cgtatcaccg tacgccttgc aaatggtcta gatgctatcc agccacaaga tggtattaca
    14161 gctgtcacga ttgcgggtat gggaggttca ctgatccgag atatcctaga agctggcaag
    14221 ttgaatcaac gcttaagcgg acaagaacgt ttgatccttc agccaaacat cggagagcgt
    14281 acattacgat cttggctgca agaaaatcag tataaaatcc aatcagaaaa aatacttgaa
    14341 gaaaataaaa aaatatatga aatcattgta gccgaaaaag aacaaaaacc agttacttac
    14401 aatgaaatgg agctgatgtt cgggccattt ctgcttgaag agaaaagtga gatcttcaaa
    14461 tcaaaatgga caagagagct tcaacaaaga aaagctatcc gtgaacagct caaacaagca
    14521 gctaatcaag aggcacgtca aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt
    14581 ttaaagccat gacaatcagc ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc
    14641 agtggctagc tgaagaagga gacccagtcg gactgcatat cggaacattg gataagccaa
    14701 tccaacgagt aatgatgaca ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga
    14761 aaaaaatcga tttgctgatt gctaaacatc ctccaatctt tcgtcctgta aaacggctgg
    14821 ttactgatca gccacaagaa aaaatgtatg cagatttgtt gaaacatgat attgccgtct
    14881 atgcagcaca cacgaacatg gatattattt gggatggact gaatgactgg ttttgtgaac
    14941 ttctcggtat tgaagtggag agttatttag taaagactca tgagattcat tataaaaaac
    15001 tggccgttta tgtacctgtg gatcatgcac agaaaatgcg tgaagtatta gctgcagctg
    15061 gtgctggtac acaaggtgat tataccggta caagttttac ctcgattgga cacggtcgat
    15121 ttactccgga agctggtgca caaccagcga ttgggaaagt agggaagaca gaacaagttc
    15181 aagaggcaaa agttgaggtt attttgccgg aaactatcga aaaacaagtg attcaagcca
    15241 tgcggtcagc tcatccttat gaggaacctg cttatgatct tttcgctata gatgaaccag
    15301 tcgaaatgtt tgggttagga cgagtaggtg aattgccgca agaaatttct atagaggcat
    15361 ttgtagaaca agtaaaagaa gcgttccaac tagatggact gagaatagtc cagccaaaaa
    15421 atgcaaaatc ctctgtgaag cgcattgcga tttgcggtgg aagcggtgaa aaattttatc
    15481 cgcaagcaat cgcccaaaga gcagatgtct atatcacggg agatatctat tatcatacgg
    15541 ctcatgatat gcaaagcgct gggttgattg caattgatcc cggtcattat attgaatcgt
    15601 tatgcaagca acgatttatc gagaaatttg aatcttggaa acaagaagaa aattgggata
    15661 ttaacttttt cgtttctgaa acaaatacaa acccatttca atttaattaa aaggagagaa
    15721 tactatgtac gaaaatcttt tgccacgttt tttacgatat gtcaaaacag aaacacgctc
    15781 cgatgcaaca agcaaaacga ctccatcgac tcagacacaa gttgcttttg cgcaagaatt
    15841 gaaaaaagaa ttagaagagt taggcttagc tgatgttttc tataatgaag ataacggatt
    15901 cgttatggga acattaccaa gcaatgttga tcatgaagtg cgttccattg gatttatttc
    15961 ccacatggat acagcagatt ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta
    16021 tgatggcgag tctacgatcg tactagatgc ggaagaacgc tatacattga atacaaaaga
    16081 ttttccaaat ttgaaaaatt atgaaggcca cacattgatc acaacagatg gcagtacatt
    16141 actgggagca gatgacaaat cagggattgc ggaaatcatg actgcaatgg agatcttgat
    16201 caataatcct tctatccctc acggtaccat caaagtggct tttggtcctg atgaagaaat
    16261 cggtgtcgga gcggataaat tcgatgttga gcaattcgat gtagattttg cttatacagt
    16321 cgatggtggt cctttaggtg agttgcagta tgaaacgttt agtgctgctc aagcaaatat
    16381 tacgatccaa gggaaaaatg tacatccagg gacggcaaaa gatacaatga tcaatgctct
    16441 acagttagca atcgattttc ataatcagct tccagccgat gaagttccag aaaaaacgga
    16501 aggatacgaa ggtttcttcc atttgatggc attggatggg aatcctgaag aagcttctat
    16561 gagttatatc attcgcgatc atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac
    16621 acatatccaa gaagagttga atcagcgatt tgatcaagaa cgaatcaaag tagatatgta
    16681 tgatcaatat tacaacatga aagaaatcat tgaaaaagat atgagcatcg tggaattagc
    16741 gaaacaagca atgatcgagc ttggtatctc tccagtgatc gaaccagttc gtggaggaac
    16801 agatggttca aaaatctctt acatgggtat tccgacgcca aatctatttg ccggaggaga
    16861 aaatatgcac ggacgattcg aatttgtttc tttacaagta atggaaaaag caacagacgt
    16921 gattgtaaaa attgcagaat tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga
    16981 caaggcgttc gtcccaatga tggaatgtca tctgtctaag gctaaagggc aggacaatgg
    17041 aattggttgt cccgccctct tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg
    17101 ctagtttttt ataacaaaag ttcagggaaa gatgagggtg agaaattagc acgctggttt
    17161 caagattatg taaaagaaaa gcagccagaa ttatatgtag aactagctga aactggccca
    17221 tcgattgatg atgcggaatt agtcaagaaa gcagaagcca tagaagctga tactttagta
    17281 attataggtg gagacggcac gattcatcat atcgtccaag cgttccaaaa aacattatct
    17341 caataccatg tcggattact tccaggtgga actgtcaaca accttgcacg tgttcttgag
    17401 attcccctca aaaaagaaga agctgctgac gtgatcctgg aaggcaatgt acggaaaata
    17461 gactatggtc aagtcaatga tgaagtcatt atctctacac tgacaatcgg aatcttagca
    17521 gatacagcag tttggatcag tcaagaagaa aaacagcgtt acggctcgtg gatctttatc
    17581 aagaagtttt ttaagttatt gctgaagaag aaaaaatatc atttggattt agaaacagac
    17641 gaggagcatt ggaaaggaaa atcccaactg ttgacagtca cgatgtctaa ctccgtgggc
    17701 ggttttacaa attttgacga ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc
    17761 gtaccttcct tagatatttt ccgatttatt ttttatttgc ctagaatcat gagaggcaaa
    17821 ttctatacga tccccggcat tacttatttc acagccaaac gcatgaaaat ccaagcgcag
    17881 gaaaaatctg tgggcactcg aacggatgga gacacgacag atgatcttcc agttgaatta
    17941 tctgttgtat caaaaggatt aacagtttat tcaccaaaga aagaagaaaa agcttagaaa
    18001 tagtagttaa cagaaagttg gaagaagagt aatggatgta ttgttacaca atcctttgtg
    18061 gttttacctt ttcattgtga atttttactt attttgtctg atggggtatg ataaacatca
    18121 ggctagaact agcggctggc gtgttcctga gaataatttg atatttgtca gcctgttagg
    18181 cggtggtttg ggaggattga tctctcgaaa agttttccat cataaaactc ggaaaaagaa
    18241 gtttactata ggattcgctg caggtgtact tgttgatttg attctcattt attttttcca
    18301 ttaaggagca ggagatgtcg tctaaaggaa cgaaaactaa aatcattctg aatctgcttt
    18361 tgctgattgt gatttttgcc attatttatt atttaatcaa tcaatcattt gcagatatct
    18421 ttgccgaatt gctgtctacc agtgttgttg ttttggcagc ggctgttata ttagggacag
    18481 tttaccaaat cgctgaaggg cgctcaatca aagaaattgc tgaacctttc gcgccgaatt
    18541 ttacaacgaa agatgggttc ttgacctcta gctatattgc gttttaccga atcgtttcgt
    18601 ttggtacagg aacgctgatc tcggagattt atttttataa caaaaaagga atgaagctat
    18661 cccagggagc aggggtgaca gcactccata tgattatgta caaactggca gtgatcactt
    18721 atgcattagc tggcttggtc atccaattct ctctttttta ttctaatggt ccatcaatga
    18781 tctggtttat catcgctggt atccttttga cgatcgtgat tgttgccttt ttattggcga
    18841 tgtccgtcag cttgaatgtt caaatatttt ttgtcagctt atcgaataag ttatttaaaa
    18901 gtgagcgttt gcggaaatgg gtggatacat gcaatcagca gatctactct ttaagggaaa
    18961 cggtccaaac cattatccaa gacaggactg cgctattgcg gatttatcta tggaatatgt
    19021 taaaacttgt attttggtat gtacttcctt atttgattct gattgaaaac catcctaaaa
    19081 tagattttct actgaccatt tcgtttatca gctttgcagt agtgctttca ggggttattc
    19141 ctacgcctgc tggtattggt tcctttgaat ttgtctattt gttattattc agaccagttg
    19201 ttggaacagt tgacgcagtt tcttcgcttt tactgtatcg tttcagttcc tttatcttgc
    19261 catttctcta tggatttgtg tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa
    19321 taatagaagt aaaaaaagag aataaagaaa atcaagggta ggtcttttat aagatctacc
    19381 ctttttatca aatcaaagaa aaacgttatg gataagataa ttttacgttt gtttggctct
    19441 ttttttatat acatacaatc aattttgttt ttttaagatt tatataagtc gagaaaaaaa
    19501 gtcaaaaaac tatgtgtata ttttgaccaa aattgaccaa agcgatataa tagtttttgt
    19561 aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt
    19621 gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact
    19681 cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat
    19741 tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt
    19801 gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac
    19861 ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc
    19921 gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg
    19981 ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa
    20041 gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta
    20101 gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc
    20161 ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat
    20221 cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa
    20281 cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat
    20341 atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca
    20401 gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac
    20461 aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca
    20521 atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa
    20581 tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca
    20641 acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat
    20701 ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt
    20761 actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt
    20821 cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag
    20881 ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg
    20941 aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag
    21001 tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa
    21061 gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg
    21121 cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa
    21181 gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt
    21241 ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt
    21301 aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc
    21361 agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct
    21421 ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag
    21481 aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa
    21541 catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt
    21601 caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa
    21661 aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact
    21721 gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc
    21781 ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct
    21841 gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac
    21901 gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg
    21961 gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa
    22021 gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa
    22081 cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt
    22141 atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa
    22201 gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca
    22261 caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa
    22321 aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt
    22381 tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca
    22441 tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc
    22501 catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt
    22561 catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc
    22621 agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc
    22681 tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc
    22741 atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa
    22801 gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt
    22861 gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac
    22921 gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata
    22981 gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat
    23041 cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag
    23101 aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt
    23161 gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc
    23221 gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg
    23281 atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca
    23341 acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat
    23401 tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt
    23461 ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat
    23521 aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg
    23581 tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt
    23641 cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc
    23701 agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat
    23761 gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa
    23821 gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga
    23881 gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa
    23941 ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata
    24001 aagttccata agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat
    24061 atttcttcag ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag
    24121 acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca
    24181 ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct
    24241 ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt
    24301 gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag
    24361 taggggatac tgtaaggata caaataagag atccattgct tattcttgcg catattatcc
    24421 aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca
    24481 actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag
    24541 atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc
    24601 aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg
    24661 tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct
    24721 tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct
    24781 ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac
    24841 taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat
    24901 gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa
    24961 tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga
    25021 aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt
    25081 ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa
    25141 aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta
    25201 tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca
    25261 aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat
    25321 cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga
    25381 aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg
    25441 gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga
    25501 cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt
    25561 tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat
    25621 gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc
    25681 tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca
    25741 agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc
    25801 aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg
    25861 ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt
    25921 cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct
    25981 tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac
    26041 tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa
    26101 cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc
    26161 acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg
    26221 gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat
    26281 tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta
    26341 tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat
    26401 cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta
    26461 tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga
    26521 ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt
    26581 gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt
    26641 agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca
    26701 agaagccttc atggccgttc tgaaaatgga tgagcaagat agtgtgaaat aacttataat
    26761 caatcgaaaa acctattaaa taaacctttt tgagtagatg caacactgag gattatctgt
    26821 ataaaagatt ttaaactgtc atttggttat cacggcgata ctaaaattaa caaaattgga
    26881 atatttttct tgaagatttt tagctgtcag tatatgatga aagcatctca taggaggtgc
    26941 ttttttcatg gttgataatt caagtggaag ggttcttaaa tctctaagaa aagagaaaaa
    27001 actatctcaa aaaaagttag ctgatttagc aggtatttca caaagtactt tagttaaata
    27061 tgaaaaaggg tcaagaaaaa tccctaaaga cgtagataat actctaagca agattctgaa
    27121 tatagaaact ctaataaaag atgaggaaga taagattgag attctcatcg gtaaactaat
    27181 tgcatacaga gatatgaata agttacttaa caaagagtta gctgataata taggaattag
    27241 tgaagtatta ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa
    27301 aaaaatcgat atttttctat taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga
    27361 tagtgagata tttagtttat caaaagacga taaaattgtt atgggaaaaa gaatacgaga
    27421 agtccgaaag aaccgggaag agacgcttga aaaatttggt aaaaacttta ccatctatac
    27481 aggcaaaaat gttataagta gatgggaaaa aggtattaat attccagata ttgaaaaact
    27541 aatgaatatt gcctatcttg gtaaggtcac tgttccttac cttatgtacg gagaagatta
    27601 taagaatata cttccaaaag atgaaagagt tagcgatttt aaaaagataa attcttttag
    27661 tatgggatta aggatgagga aaattaggaa agattattat ctagaaaggg aggagtttgg
    27721 aaagcttttt tctccttcaa tttcaaaatg gtccatagat aggtatgaga atggtagaga
    27781 tatacccaat actaatcgaa ttatccaata tgcttatata ggaaatcttt ctttagaatt
    27841 tttgatttac gggatataaa aatagtttac gtcgatactt attaagttat cgacgttttt
    27901 tattatagaa aaaatttata aaaggaggtt tcttgatatg ataagaaaca aaaagggttt
    27961 gttgaaagtt gctttactgg cagctttttt ctaagtaact ttttgacaag ctcaatggca
    28021 acacaagcgg aagaagtaat acaagaacta actgttatga ataacagcta gataaaatgg
    28081 gctatgtatg ttatccaaca atttagtaat cactagaagc ctagcgaaag ttcgcttgat
    28141 aagttataga tgagtattga tttgtagtgt ttccaaatca aaagggtaca gagccacatg
    28201 aaatatgttg tcctctgcag cgtcaggatg ggaatccata ccatggagat tcggagatct
    28261 gtgagggaat acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt
    28321 gggtagattg actgaattct tatatgagaa aatgaaacca acaagaagat agggttattt
    28381 atacagaatt ggggatcttt tctggaaaag taatcggata atgcgagatt cgtacggtct
    28441 tttttaaatt tctctaaaaa aaacggcatc gaatgggttg ggtgattgat aagcaatgaa
    28501 tcatagactg atatagaata aaaaatgatt ctatttatct ttttacatct atcgtttttt
    28561 caagtagccg caaattatat cagcattcaa caatttttgt atacaagttc tagtaaaagg
    28621 atctgaagaa aaagttggtt gttgtgaaga taaaaaaaga atcaggtttt actgagtttt
    28681 tcttcaaaat ttgtgttatt aggttaggac aaagataaga agaagtatga agatttaaaa
    28741 aaggaatgag gaaaagatag taatttccga ttattggagt ttttttat
//
LOCUS       DADQRX010000027        28603 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.027, whole genome shotgun sequence.
ACCESSION   DADQRX010000027 DADQRX010000000
VERSION     DADQRX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.027"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..1877
                     /locus_tag="J8V14_001110"
     CDS             237..1877
                     /locus_tag="J8V14_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBA0921229.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="J8V14_001111"
     CDS             2031..3353
                     /locus_tag="J8V14_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBA0921230.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="J8V14_001112"
     CDS             complement(3415..3846)
                     /locus_tag="J8V14_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0921231.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="J8V14_001113"
     CDS             complement(3910..4746)
                     /locus_tag="J8V14_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBA0921232.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="J8V14_001114"
     CDS             complement(4936..5238)
                     /locus_tag="J8V14_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBA0921233.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="J8V14_001115"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="J8V14_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBA0921234.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="J8V14_001116"
     CDS             complement(5692..5958)
                     /locus_tag="J8V14_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBA0921235.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="J8V14_001117"
     CDS             complement(6212..6937)
                     /locus_tag="J8V14_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBA0921236.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="J8V14_001118"
     tRNA            complement(7303..7373)
                     /locus_tag="J8V14_001118"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="J8V14_001119"
     CDS             7490..8242
                     /locus_tag="J8V14_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBA0921237.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="J8V14_001120"
     CDS             8258..8737
                     /locus_tag="J8V14_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBA0921238.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="J8V14_001121"
     CDS             complement(8988..10568)
                     /locus_tag="J8V14_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBA0921239.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="J8V14_001122"
     CDS             complement(10561..11304)
                     /locus_tag="J8V14_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921240.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="J8V14_001123"
     CDS             complement(11480..12256)
                     /locus_tag="J8V14_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0921241.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="J8V14_001124"
     CDS             complement(12504..13340)
                     /locus_tag="J8V14_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBA0921242.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="J8V14_001125"
     CDS             complement(13351..14280)
                     /locus_tag="J8V14_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA0921243.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="J8V14_001126"
     CDS             14472..14825
                     /locus_tag="J8V14_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBA0921244.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="J8V14_001127"
     CDS             complement(14899..15555)
                     /locus_tag="J8V14_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBA0921245.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="J8V14_001128"
     CDS             15781..17715
                     /locus_tag="J8V14_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBA0921246.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="J8V14_001129"
     CDS             complement(17801..19024)
                     /locus_tag="J8V14_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HBA0921247.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="J8V14_001130"
     CDS             complement(19018..20916)
                     /locus_tag="J8V14_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBA0921248.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="J8V14_001131"
     CDS             complement(20910..22028)
                     /locus_tag="J8V14_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HBA0921249.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="J8V14_001132"
     CDS             complement(22111..22944)
                     /locus_tag="J8V14_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921250.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="J8V14_001133"
     CDS             complement(22937..23854)
                     /locus_tag="J8V14_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921251.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="J8V14_001134"
     CDS             complement(24081..25205)
                     /locus_tag="J8V14_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBA0921252.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="J8V14_001135"
     CDS             25388..26296
                     /locus_tag="J8V14_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921253.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="J8V14_001136"
     CDS             26437..28050
                     /locus_tag="J8V14_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBA0921254.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DADQRX010000028        26915 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.028, whole genome shotgun sequence.
ACCESSION   DADQRX010000028 DADQRX010000000
VERSION     DADQRX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.028"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="J8V14_001137"
     CDS             complement(164..388)
                     /locus_tag="J8V14_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBA0921255.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="J8V14_001138"
     CDS             complement(586..2166)
                     /locus_tag="J8V14_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBA0921256.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="J8V14_001139"
     CDS             complement(2567..3943)
                     /locus_tag="J8V14_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA0921257.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="J8V14_001140"
     CDS             complement(4071..5189)
                     /locus_tag="J8V14_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA0921258.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="J8V14_001141"
     CDS             5331..5765
                     /locus_tag="J8V14_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0921259.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="J8V14_001142"
     CDS             complement(5843..6376)
                     /locus_tag="J8V14_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBA0921260.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="J8V14_001143"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="J8V14_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBA0921261.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="J8V14_001144"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="J8V14_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBA0921262.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="J8V14_001145"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="J8V14_001145"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBA0921263.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="J8V14_001146"
     CDS             complement(10101..10679)
                     /locus_tag="J8V14_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HBA0921264.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="J8V14_001147"
     CDS             11001..11471
                     /locus_tag="J8V14_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBA0921265.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="J8V14_001148"
     CDS             complement(11579..12805)
                     /locus_tag="J8V14_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HBA0921266.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="J8V14_001149"
     CDS             complement(13559..13759)
                     /locus_tag="J8V14_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA0921267.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="J8V14_001150"
     CDS             complement(13893..14501)
                     /locus_tag="J8V14_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBA0921268.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="J8V14_001151"
     CDS             complement(14586..15056)
                     /locus_tag="J8V14_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBA0921269.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="J8V14_001152"
     CDS             complement(15104..16018)
                     /locus_tag="J8V14_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBA0921270.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="J8V14_001153"
     CDS             16164..16967
                     /locus_tag="J8V14_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBA0921271.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="J8V14_001154"
     CDS             17145..18092
                     /locus_tag="J8V14_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBA0921272.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="J8V14_001155"
     CDS             complement(18160..18453)
                     /locus_tag="J8V14_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBA0921273.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="J8V14_001156"
     CDS             complement(18481..19146)
                     /locus_tag="J8V14_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBA0921274.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="J8V14_001157"
     CDS             complement(19286..19918)
                     /locus_tag="J8V14_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0921275.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="J8V14_001158"
     CDS             complement(19925..20992)
                     /locus_tag="J8V14_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA0921276.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="J8V14_001159"
     CDS             complement(20989..21720)
                     /locus_tag="J8V14_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBA0921277.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="J8V14_001160"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="J8V14_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBA0921278.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="J8V14_001161"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="J8V14_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBA0921279.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="J8V14_001162"
     CDS             23883..25052
                     /locus_tag="J8V14_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBA0921280.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..26233)
                     /locus_tag="J8V14_001163"
     CDS             complement(25319..26233)
                     /locus_tag="J8V14_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921281.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(26291..26915)
                     /locus_tag="J8V14_001164"
                     /pseudo
     CDS             complement(26291..26915)
                     /locus_tag="J8V14_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8233 a         5317 c         4285 g         9080 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa
    25921 atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc
    25981 atggaaatat ttttgtacaa agtagaatag atattatacc cattatataa gtgattttag
    26041 ataagattcc taatttttta aagttaaatg ttagtataat taaacaaata agcaaagtta
    26101 agccaatacc aaatgatagt aattctgaat ctatacttcc aatcaaaaac tctttaccca
    26161 gataaattgc tcttttttgt aatatatcca cataaggtct attcagatct tgatacaatg
    26221 tctgctcaat cataggacct ataaacgcta gtgaaagaag taaagtaact atagcagctt
    26281 ttattaattt aataattaag aagatttcta ttttcttttt ccgataaaat tgaataatta
    26341 aagcaattat aatcattacc atagtcatag ctacagaaag aacatgagtg taaattaata
    26401 aagtcatgct aatcgccaac ataacccaag attctttttt tgagtcgtaa agctttataa
    26461 acccgagtaa aataagagga agtattgaca ttgcgagtaa ttcccccatt gcccctcgaa
    26521 gaaatatttc tacactccta aaagaagatg ttgtatatat aatagcgaaa aaggagctag
    26581 taacatgttt tttggtaatt tcatacatta cataatgaca tataaataga gtaatgagcg
    26641 taacaatata aagaaaaagg ttatagccta ttgctaaatt agtaaacttg atcaataaaa
    26701 ataaaggata tagagttaac catgggtaaa aattatttac ttgcatgcca tatgatctaa
    26761 acgtttcaaa attaataggg gagctaaaaa tagattctaa acttaacgca cgattaatgt
    26821 gaaatgccgt atcataaaag ccaaatattt ccatgttttt aaatgctgga aataaatata
    26881 ataaactaac aaatgcataa actataaata ttttt
//
LOCUS       DADQRX010000029        26748 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.029, whole genome shotgun sequence.
ACCESSION   DADQRX010000029 DADQRX010000000
VERSION     DADQRX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.029"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="J8V14_001165"
     CDS             complement(104..1336)
                     /locus_tag="J8V14_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA0921282.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="J8V14_001166"
     CDS             1591..2160
                     /locus_tag="J8V14_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0921283.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="J8V14_001167"
     CDS             complement(2338..2778)
                     /locus_tag="J8V14_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBA0921284.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="J8V14_001168"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="J8V14_001168"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBA0921285.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="J8V14_001169"
     CDS             3732..4655
                     /locus_tag="J8V14_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBA0921286.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="J8V14_001170"
     CDS             4731..5870
                     /locus_tag="J8V14_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBA0921287.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="J8V14_001171"
     CDS             5863..6663
                     /locus_tag="J8V14_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA0921288.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="J8V14_001172"
     CDS             6663..7490
                     /locus_tag="J8V14_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA0921289.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="J8V14_001173"
     CDS             7468..8202
                     /locus_tag="J8V14_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA0921290.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="J8V14_001174"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="J8V14_001174"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBA0921291.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="J8V14_001175"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="J8V14_001175"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBA0921292.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="J8V14_001176"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="J8V14_001176"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBA0921293.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="J8V14_001177"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="J8V14_001177"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBA0921294.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="J8V14_001178"
     CDS             11787..13820
                     /locus_tag="J8V14_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921295.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="J8V14_001179"
     CDS             13864..15144
                     /locus_tag="J8V14_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBA0921296.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="J8V14_001180"
     CDS             15354..16160
                     /locus_tag="J8V14_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921297.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="J8V14_001181"
     CDS             16172..17392
                     /locus_tag="J8V14_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921298.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="J8V14_001182"
     CDS             17382..18968
                     /locus_tag="J8V14_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0921299.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="J8V14_001183"
     CDS             19007..21145
                     /locus_tag="J8V14_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA0921300.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="J8V14_001184"
     CDS             complement(21513..22496)
                     /locus_tag="J8V14_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBA0921301.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="J8V14_001185"
     CDS             22874..24265
                     /locus_tag="J8V14_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBA0921302.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="J8V14_001186"
     CDS             24281..25321
                     /locus_tag="J8V14_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA0921303.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="J8V14_001187"
     CDS             25340..26425
                     /locus_tag="J8V14_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBA0921304.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26748
                     /locus_tag="J8V14_001188"
     CDS             26458..>26748
                     /locus_tag="J8V14_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HBA0921305.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGAD"
BASE COUNT         8574 a         4446 c         5435 g         8293 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta ttagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgat
//
LOCUS       DADQRX010000030        26696 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.030, whole genome shotgun sequence.
ACCESSION   DADQRX010000030 DADQRX010000000
VERSION     DADQRX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.030"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..738
                     /locus_tag="J8V14_001189"
     CDS             <1..738
                     /locus_tag="J8V14_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBA0921306.1"
                     /translation="NPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVA
                     DRFHISQHIGRAFTNHRIQVMKTFKKGDRRSKHLKKYWRLLQKNAWELNGQHRYWRPS
                     FRDHLTEAEIVDRLLYYDDSLKRGYEVYQVFLSAIRRQDVPEFVALLKEDYKELPEHY
                     QPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYRERIFLYR
                     GKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(979..1386)
                     /locus_tag="J8V14_001190"
     CDS             complement(979..1386)
                     /locus_tag="J8V14_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBA0921307.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(1575..2402)
                     /locus_tag="J8V14_001191"
     CDS             complement(1575..2402)
                     /locus_tag="J8V14_001191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBA0921308.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            2711..2929
                     /locus_tag="J8V14_001192"
     CDS             2711..2929
                     /locus_tag="J8V14_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921309.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2950..3585
                     /locus_tag="J8V14_001193"
     CDS             2950..3585
                     /locus_tag="J8V14_001193"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0921310.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3778..4017)
                     /locus_tag="J8V14_001194"
     CDS             complement(3778..4017)
                     /locus_tag="J8V14_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921311.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4041..5564)
                     /locus_tag="J8V14_001195"
     CDS             complement(4041..5564)
                     /locus_tag="J8V14_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBA0921312.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(5582..5704)
                     /locus_tag="J8V14_001196"
     CDS             complement(5582..5704)
                     /locus_tag="J8V14_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBA0921313.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(5734..6717)
                     /locus_tag="J8V14_001197"
     CDS             complement(5734..6717)
                     /locus_tag="J8V14_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBA0921314.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6741..6890)
                     /gene="rpmG"
                     /locus_tag="J8V14_001198"
     CDS             complement(6741..6890)
                     /gene="rpmG"
                     /locus_tag="J8V14_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBA0921315.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6911..7288)
                     /locus_tag="J8V14_001199"
     CDS             complement(6911..7288)
                     /locus_tag="J8V14_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921316.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7320..7499)
                     /gene="rpmF"
                     /locus_tag="J8V14_001200"
     CDS             complement(7320..7499)
                     /gene="rpmF"
                     /locus_tag="J8V14_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBA0921317.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7514..7783)
                     /gene="rpsN"
                     /locus_tag="J8V14_001201"
     CDS             complement(7514..7783)
                     /gene="rpsN"
                     /locus_tag="J8V14_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBA0921318.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8306..10078)
                     /locus_tag="J8V14_001202"
     CDS             complement(8306..10078)
                     /locus_tag="J8V14_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921319.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10032..11786)
                     /locus_tag="J8V14_001203"
     CDS             complement(10032..11786)
                     /locus_tag="J8V14_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921320.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11896..12465)
                     /locus_tag="J8V14_001204"
     CDS             complement(11896..12465)
                     /locus_tag="J8V14_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBA0921321.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12483..13907)
                     /locus_tag="J8V14_001205"
     CDS             complement(12483..13907)
                     /locus_tag="J8V14_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA0921322.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13909..14577)
                     /locus_tag="J8V14_001206"
     CDS             complement(13909..14577)
                     /locus_tag="J8V14_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBA0921323.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14708..15292)
                     /locus_tag="J8V14_001207"
     CDS             complement(14708..15292)
                     /locus_tag="J8V14_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0921324.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15627..15857)
                     /locus_tag="J8V14_001208"
     CDS             complement(15627..15857)
                     /locus_tag="J8V14_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBA0921325.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(15872..16822)
                     /locus_tag="J8V14_001209"
     CDS             complement(15872..16822)
                     /locus_tag="J8V14_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBA0921326.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16819..17661)
                     /locus_tag="J8V14_001210"
     CDS             complement(16819..17661)
                     /locus_tag="J8V14_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBA0921327.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(17794..18519)
                     /locus_tag="J8V14_001211"
     CDS             complement(17794..18519)
                     /locus_tag="J8V14_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBA0921328.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18980..19294)
                     /locus_tag="J8V14_001212"
     CDS             complement(18980..19294)
                     /locus_tag="J8V14_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBA0921329.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(19310..20905)
                     /locus_tag="J8V14_001213"
     CDS             complement(19310..20905)
                     /locus_tag="J8V14_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBA0921330.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20928..21818)
                     /gene="iolE"
                     /locus_tag="J8V14_001214"
     CDS             complement(20928..21818)
                     /gene="iolE"
                     /locus_tag="J8V14_001214"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBA0921331.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21835..22860)
                     /gene="iolG"
                     /locus_tag="J8V14_001215"
     CDS             complement(21835..22860)
                     /gene="iolG"
                     /locus_tag="J8V14_001215"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBA0921332.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22884..23891)
                     /locus_tag="J8V14_001216"
     CDS             complement(22884..23891)
                     /locus_tag="J8V14_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0921333.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(24004..25164)
                     /locus_tag="J8V14_001217"
     CDS             complement(24004..25164)
                     /locus_tag="J8V14_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA0921334.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(25240..26694)
                     /locus_tag="J8V14_001218"
     CDS             complement(25240..26694)
                     /locus_tag="J8V14_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA0921335.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         8202 a         5479 c         4131 g         8884 t
ORIGIN      
        1 aatcctttac gcgactattt cttgcgctac cctagaaaag tccgtgaaca agtacgtcta
       61 gtggtttcag atttctatac accctatcgc acattggtga aagagtgttt tccgaatgcc
      121 cgaatcgtcg ccgatcgctt tcatattagc caacacatcg gtcgggcctt taccaaccac
      181 cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa
      241 tactggagac tacttcaaaa gaatgcgtgg gaattgaatg gtcagcatcg ctactggcgt
      301 ccgtcatttc gagatcatct gacagaagcg gagatagtcg atcggctatt atattatgat
      361 gactccttaa agcgaggcta cgaagtctac caggtttttc tttccgctat cagaagacaa
      421 gatgttcctg aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactat
      481 caaccagtgt tcacgacctt caagaaatat cgaacagaaa tcaaacgagc gttgcgagtt
      541 ccctattcca acggccctat agaatgtttg aacaaccata tcaaagtact aaaacgaatt
      601 gcctatggct ttagaaactt tcaaaactac cgagaacgaa tctttttata tcgaggaaaa
      661 tatttcaaga aaactaagaa cactacccaa ctgactaaag ccagaacaac aacacggcta
      721 gacaagatag ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa
      781 cactcattga cgaagagcca aaataagtaa tctaaccgac tgttaactaa accattgcag
      841 gtcccgttaa agatacttgt gcctgttgtc accgcttctt tttcttgtcc acaagcgctt
      901 aacacactta ataaagcccc tgctattatt aataataata tctttttctt attaaaaacc
      961 tcctcatcta tttccagatt agtaaatgta gtaatctttg ctttcttttt tctcaatact
     1021 aataactaac ttatggggta aacaaatact cgtttgaccg ggttttgaga tccatccagt
     1081 tctaaccgcg atttgatcag gactattatc ttctttaact cgaattcttc catctttaat
     1141 ctcaacaata ttatattgtc ccttcgcagg atgataattc acgagtttat gattgatctt
     1201 atccaaatcg aagcgatcca tttccttgcc atcaatttga acgatggcat atttgatagc
     1261 atcttcagtt gtttcattct ccttttctat agagaataca aataagggaa taaaagaggg
     1321 aataatcaaa atgccaataa ttatgacatc gcctatcttg acaccagctt ttcttaaaaa
     1381 tttcattaac gatatctggc taaacatatt agaatttttc catggaaatg gtgaaatttt
     1441 tcggaaatta tctgcattaa gagaatcagt taaacaaccc tcaagttgtt ccttaataca
     1501 ttgttgatcc atcttcatac caataaaaac tagtttatta atgcgttcac caaacaacgg
     1561 atgccaggct tccttcaatt cgggtttttt tgtataatca tttgacactc aattttaggt
     1621 aaactagcta cccaataatt taatgattca atacttttat ggctacccga tgtttcaaag
     1681 atcgtcgaaa aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg
     1741 ccaggaaaat ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt
     1801 cgatagataa aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca
     1861 agctctaact ctttgaacca gcccattgaa taggaagtct catctatatt aaaaagattt
     1921 gtatttagaa ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg
     1981 gactgtaact tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca
     2041 ctattattta aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata
     2101 tcctgttctt cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt
     2161 tcgaaattat cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat
     2221 cgttgggtca aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga
     2281 attggtttgc tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact
     2341 aattgatcta gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc
     2401 atcgaaacaa gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca
     2461 acatttactt cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac
     2521 agaatatgat ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata
     2581 ggtatttttt catagggaac ctctttctat accttgaatt tacactttaa gtgcaacaca
     2641 taaaaatgag tcataatcga tatccaacta gtataagttt gtcgagaaca ctcctagaag
     2701 gaaaataatt atgagccagt attgtcatct tactatactc aaacgagaaa gaatctttct
     2761 gcttcacgaa gaaggatttt ctattagtat aattggcaac gacattcagc atagcccgtt
     2821 tactatttta catgaactga gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa
     2881 aaaaattatg cttatcgaag aaaaaaatgc ggacgttatc acattctaaa tgatccgtga
     2941 gtttggtcaa ttgttcgtca tttatttgtc gattttcagt ggtcgccaga gaaaatctcg
     3001 aatcgtttga agcatgaaca tatggacttt tcaattagtt ttatcacgat ctaccgtgca
     3061 atttgcaatg atcttttcga tacagaaccc ctctcacacg gcaataaagg cctgatccgt
     3121 tcattgcacc atcgaggaaa aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag
     3181 atttgcctta ctcattcgat ccatggccat ccaaaatccg cagatgatag aagtgatttt
     3241 ggtcattgga aagccgatat tgtagctggc aaaactagtg gtacttgttt agtgactttg
     3301 acagacagaa aatcatgctt ccttttagca ggaaagaagg tccccagaaa gaaagccgct
     3361 tatgtgagcg agaaaatgat tgaactttta agcagtattc caaatgaggc ggtcaaaacg
     3421 atcactccag atagaggaaa agaattctcc aatcatttcg cagttacaga agtattgaat
     3481 aatatgccct tttattttcc agatcctcat gcccttggca acaatgaaca aattaaaata
     3541 cgaatagact cattcgagaa tacttgccaa aatcgaaaga gatagctacg gtagataact
     3601 caacgataaa tcagatgatt gaaaaactga ataacagacc tagaaagtgt ttagattgga
     3661 gaacacctta tgaagtcttc tttgataaag tgttgcgctt gatttgacaa ttcaagaaat
     3721 aataaaaagc agatcaaatc caaataaggg aaaaatgatc tactctggta ttatcaatta
     3781 attggccaag ctattatgcc gttttgctaa acgtctactt cttccagtat tgaccttgat
     3841 aaaacaacaa tcaggatccc gttttaatac tctatgaact gtgtcaatac taacatgtaa
     3901 agttgcagca atatcagcta atgtactccc atttaatctc atttgctttg caaattgata
     3961 acgccgataa gcatcaatat tcttggaatc catagacaat aatacttttc ttaccatgta
     4021 tttcctccaa agattggatt ttaatgggcc atcatttctt gagcaatctc atgctcagat
     4081 aatgaagctg gatagaaagt aggccaatta tgcatttcat tataaagttt ttcttgactt
     4141 tctccaccaa agaaaatatg gaaatgctct gccttacttg gtgcaattcc atgatcgcta
     4201 aattgaatat atttaaaacg accagcacct gcatcatcag tttcaaaatt aaaacgaaca
     4261 ccacggtttc ctttttcata attcaaaatt ttgtatccaa catatttgta tgtgtattga
     4321 tgatgttcac cgttgactac aaaatcaatg gtgttgtctt taatgttgat agtatcaata
     4381 tctgtcttat agccagtagt ataataatct ttgtactcgg cagctgtcat gtctttattg
     4441 agttttgctt tgtaatcgaa aacttcatcc aaaattccat cttttaacaa cggataaaca
     4501 gattgccatt cgcctgtata atcactcaat tcgcgatcct tcacatcggc atctttaaag
     4561 tacccattag caactgtctt ttcatccttc gcttcttttt ctggaactac ctcttcacca
     4621 gcaaccatag ttgttttctc taacgctttt agattgtctt ccattactga aacataatct
     4681 tcaccattat ccatttgttc ttttgtaaga ctttcaagag gattcaatac ttccaatttt
     4741 atcccagctt cattggccaa agtttttgcg attttatcat tagcattctt ttcaaagtaa
     4801 atataattga tcttattttt tttaacatac tcttttaatt cagctaatcg accagaagat
     4861 ggctcttctt caggacttag tccagcaata ggaacttgta tcaatccata atccaaagct
     4921 aaatatccaa aggctgcatg ttgtgtcaca aaactttttt gtttcgcttc tttaagacta
     4981 gtggtatagt cagcatccaa ccgtttcagt ttagtcaaat acttttcagc atttgtctca
     5041 aatacttttg cttttttcgg atacaattta actaactgat ccttgatatt tgaaacttcc
     5101 ttaattgcca ttttgggtga aacccacgta tgaggatcta gttcatgatg gtgatcttct
     5161 tctccgtgat catgatcttc ttcttcacta cctggcaata aaatcatgtc ttttgttcct
     5221 tctactacat taggcccagc tttcttccaa ctttctttag cttttggtac ccatgattcc
     5281 atgttttcgt tatgatatac aaaaacatca gcatcatgag cttttgccat atccttagct
     5341 gagggctcat agtcatgagg ttctgatccc gctggaacca ttaaatccac gttcgcttca
     5401 tcgccgacga tgtttttggt gaaatcatac attggataga atgttgttac aatttctaat
     5461 tttccatctt tatcattcga tacttttgca tcattagtgc tggagcaagc tcccaataat
     5521 cctagagctg ctatcccaat aagtcctaca ctgattttct tcattctttt tccttcctta
     5581 attatgctaa tttcttcatt ttattttgtt tatgttcttt gataattttt aatgcccttt
     5641 ttctagtttt gatattcggg cttttaagtc gtcgatatgc actagatata tccgttttct
     5701 ccattagaaa ttacccctaa tcaatactta tcattatttg ctttccgcca gtaatttatc
     5761 aaaactctgt ttgatccttt cttcatccaa catttttcca attagaataa tagtggtttg
     5821 gtctttttgt cgagtggtgt attgaaaatt aacctgttcg tttacccctt gtatggcaac
     5881 tataaaatct tgatcttgca aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt
     5941 ggttaaaatc agccggtcaa ttaattggaa taaaaaatct ttttttatgt cttgatctga
     6001 agttaagaga attgatttaa actcatgttc ttgatgatgt tgatgatagt gtttgccatt
     6061 atagtgattt tctgatgaat cactttcaaa aactaaggga tgattgaatt tttccaaatt
     6121 ataaaaatcc tttttttgaa tcgtttcact atgtgtgaac attcgaatat cggataaagg
     6181 gttaatactt ttgatttctc tctgtaattt ttttagtgca gataaattca agttttgttt
     6241 tctagacaca aaaatgcgat ctgctatgac aagttgctta agggcttgtg gttctctttc
     6301 aataagttca aaactgtcac tatcaataac agtcaataaa ctatcgatat atactcttcc
     6361 tcttagttga ggcgctgtta aaagtgtttg aatgattggt tggggatctg ctatacctgt
     6421 agtttcaatg atgatttgtg aaattggttg ttgatgttct tctattgttt ccagtaaaga
     6481 aatcaatatt gctaataaat cactgcgaag actgcaacac atacatccat tatttagttg
     6541 taacacccgt tcctcgctat gaactaataa tttgtgatca atcccaactt caccaaactc
     6601 attttcaatt ataataattt cttccctggg gtagggtgag acttgcaagg cttgattgat
     6661 tagcgttgtt tttccagccc ctaaaaaccc agaaacgatt gtaactggta tgcccatttt
     6721 gtcacctttt taccacttag ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt
     6781 ggcgaatact tttttaattc taaccgttca gggttattcc gtttattttt gctggtgaga
     6841 tacagacgtt ctccagtttc gacacattcc aaaataatat tttgacgcat aaatcttcct
     6901 ccttcaatac ttatcgatct tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta
     6961 actcgccaga ttccaattgt gaaactaaat tttctttcat atcaacaggt atctcccaat
     7021 aaccatcaat acattgaaat aactcctcgt catactcttc aaaaattgga tagattctta
     7081 aaaaatcatc aaatgataag ccttggtcct catagtaatc tttgactaat ccattttcaa
     7141 ttgcctgttg ggtcggtaaa cccgtttcag taataattcc tctctctacc atcgaacgat
     7201 aaaaagtgca tttatctagc gattgctggg tcgtttcaag ataatacttt aatccttctt
     7261 caactaaagg tgggtaaata atttccattt tgattccctc ctaaaagtta tatattaaac
     7321 tattttgaag ctcctttaat tactttcttt ccattatagt acccttttaa agatacatga
     7381 tggctacgtc tataatcacc attcgcttcg tcaaatgaaa tttctggttt tactacagtt
     7441 tgatgagttc ttcttaaccg tttcttcgcc ttagatgttt ttcttgctgg tactgccatg
     7501 atatttcctc cttttaccaa cttgcttttt ttactccagg aatcaaccct tgatgggcaa
     7561 gttctctaaa cttaatacgg gacataccga actttcgcat ataccctctt ggacgaccat
     7621 ccgtctgatc acgaagcctc agacgattcg gatttgaatc tcttggcaat ttagcgagag
     7681 cggtgcgatc tcctgcggct tttaattcct gacgtttatc tgcgtacttt tcaatcatcg
     7741 ccatttgttt ctttgcttta gcaattttcg acttttttgc catatacctt tatactcctt
     7801 ttattttgat ttagacatct caaatcgtaa tcgttacgtt ttgacactga tactttacca
     7861 acaagagttg ctcttgtcaa caataaataa catgaaataa aactcatatg aatagataac
     7921 tatttgaaaa cgaatttatc ggtacataga gataatcaaa tcatttaaaa gaatatgcag
     7981 tttaagctct gaaaaataag aattcacttt tattagtcca atcaaacaca aataccgttt
     8041 taagtagctt ttcctctact taaaacggca atatgaaaga aactataact ttttattcaa
     8101 tcttttatgt ttaactggtt tatcgccaat tttttaaaac tttgattatt ccttatatta
     8161 taagaataga cattaagtga actaattcaa tcatactgat tctttcgtgt attcaaaatt
     8221 agatgctgtt taataaaatt attctttttg aaaaagaatt ataactatat cccaaaaatt
     8281 gtaggttaat ttaaagccta tttcatcatc gtgattgagc ttctttttca tagacgaatt
     8341 tctcagatag tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact
     8401 cactatgagt accttgatca actatttttc cttgatccat cactacaatt ttatttacct
     8461 tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat
     8521 ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta
     8581 aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc
     8641 ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt
     8701 caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt
     8761 gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa
     8821 aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat
     8881 caattaaaat acagccttca tcataatcat acagtctaga cactaatttc aagatactag
     8941 ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag
     9001 agatatgatc taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa
     9061 tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc
     9121 taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat
     9181 cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca
     9241 cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg
     9301 agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc
     9361 tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc
     9421 tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttt tctctcaaag
     9481 tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa
     9541 aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca
     9601 tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca
     9661 tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct gataaattat
     9721 gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat
     9781 gcgcactttc tttataagta ctattatata agttttcata ctccctatct aacaaccaat
     9841 acatgaaaag caaagtaacg aacgatactg taaaataaag ccaaggcggc tttacatttt
     9901 ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt
     9961 atacaaaaaa gctagaaata gatgctttaa ccaaatcttc tgcgccctga ttagttaaag
    10021 cgaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa
    10081 ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg atttccacgt
    10141 tctactattt tcccttctga caagacaaga atttcatcta cagatttaat gctggataat
    10201 cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt
    10261 tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca
    10321 tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact
    10381 cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa
    10441 ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa
    10501 acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg
    10561 ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc
    10621 ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat
    10681 tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca
    10741 cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt
    10801 gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt
    10861 tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa
    10921 acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga
    10981 gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa
    11041 aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac
    11101 ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat
    11161 atctttataa cttgaattcc tcggatatat tctaccgttt cagagcttaa cacatctaac
    11221 gattcttgat atttttgaat aaaatttgta ttctctgtca ttttctttaa caagaataca
    11281 ccaattacta acatggcgat tagaaccaat ccaattttaa aattaatgaa aaagcctaag
    11341 gcaactgata aaacaggtgt tactatggct tgagcactat ctggaatcaa gtgagcaatt
    11401 gccatatgtg ttttagccgc gttgtcatca attgtctttc gaatatatcc cgaagaattt
    11461 aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta
    11521 gtctctaagc gaaacccaag gatatgagaa aataagccag aaaaaatata aaataaagct
    11581 cctaaagtta accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct
    11641 acaataatta gagacttaag aaatttgtaa atactgtaat atccatagac tgttaatacg
    11701 acagaaaaat cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa
    11761 ctaaataatt ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat
    11821 tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagatag
    11881 aaggtaaact acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc
    11941 aatcatagat aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata
    12001 gtggatcatt gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa
    12061 acgatctgta aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag
    12121 agcccaagac gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc
    12181 agctaagatc gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa
    12241 cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt
    12301 atagctaata taaacaaata tgattccaga taataatcca aaaatccctg gggatatgct
    12361 gtgaccaaac gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga
    12421 aataactgta gctaaaccaa taaaaatata atcttttgtt ttcatatata ttctcctcaa
    12481 ttctatttcg ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt
    12541 tttatatctt tttttattcc accatttttt aaataaataa ttctattgca tgttttataa
    12601 agaaattccg gatcatgagt ggctataact actgcaccct tttcttttaa gtctttaata
    12661 aggtcagcaa tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga
    12721 aataaattag tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg
    12781 gataaatcaa acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt
    12841 tcttgctgat ctttactaac tatttcattc cccagggcta actcgctcct cactgattct
    12901 gtgaaaaatt ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta
    12961 ttttcgatta tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt
    13021 gttttcccca caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa
    13081 tttatgtctt ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt
    13141 accttttcga tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg
    13201 ttattgtttt cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca
    13261 aatagaactt gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata
    13321 gtaaatcctt tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag
    13381 tccaaatttg aagacggttc atccaatata attaatttag agtttagcga taaactacat
    13441 ccaatagaaa ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata
    13501 tttgaaagat ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta
    13561 aatccaaagt tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt
    13621 cctctaggat cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat
    13681 tgtagcgagt tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga
    13741 ataagtccat tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc
    13801 aatgtaagac tacctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca
    13861 tccttatatg aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat
    13921 acttaagaaa aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt
    13981 tttatagtaa gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt
    14041 catttcttca gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata
    14101 ttgcagaggc tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat
    14161 tcttatttca gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat
    14221 ttttttcggt aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact
    14281 aaccaacaac catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt
    14341 cccaaaaaat atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa
    14401 cagaactgaa taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac
    14461 taaaactgta gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat
    14521 aatgaccgga aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct
    14581 ttcacctcca aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt
    14641 cagataacag tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt
    14701 tttttaatta actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg
    14761 ttaatagcat agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt
    14821 agaatgagta aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca
    14881 ttccgatagt ctttaactta tctagacaac agactgtctt ttttgtcaca aatgatgtaa
    14941 ttttttcaac aatatcttga tatttggttc cagctgattg gaataaaacc aaatcgaata
    15001 tacgtttatt ctcaaaaata taattaataa atggatctaa ttcattgaag ttctctctcc
    15061 aacttttctc tatatctata ttttctaagt ttaagaggta tttggtaaac tcttcattat
    15121 acatattaat aagcgcatta atatgcggct gaataatttc ttcaaataac gcttccttag
    15181 aagtaaaatg tttataaaaa gcacccgtag ttacgcctgc ggtcaagcac agttgcctta
    15241 tcttagtttg ctgaaatcct actttttcaa aatggcttag tccactttct attaattttt
    15301 ggtgagttgt aatataatct ctggtttcta tgggtacttc ctcctaataa gatgaattaa
    15361 ctatattatc ttacacagga cttgaagttt caatcgttaa cgtctattac tgaactcatt
    15421 ccttagagct aaactaatga gaaagagcaa aataactgat acaaaaagca atcctacaag
    15481 aattaataga ctcatctaaa tcattcacct tttctaaaat aatccgagaa agatttaaaa
    15541 aaattatatc cagctattag gataagtcct atcataatca ctccaatagc cattttctta
    15601 tcctccctaa aaataaagcc ttttacttaa aacctttttt tgtaatcgtc aataaatttt
    15661 ttccctttat cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagtc
    15721 agcatctcac ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt
    15781 aatttctttt ttctttttga taattcctga attgctagta aataaattag tatagtagtt
    15841 gttttaattt gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat
    15901 ccaggttcca tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg
    15961 aatcagtaaa aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg
    16021 gtctatcgtc cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt
    16081 caactaagtg ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt
    16141 aagcagaagg cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa
    16201 tcattttttt atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat
    16261 ccaatttttc aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt
    16321 tttccgctaa ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc
    16381 aagcatgggg atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc
    16441 catcacttgc tgcaaaatag tctttgtttt cctctttgtt cgcatttttt actaattttg
    16501 taaaccaagc atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt
    16561 ttgaagtttt ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag
    16621 gtacgatact gtgtaaatca attttatctt ttgctatatt ttcagtaata tctgctaaaa
    16681 tcgaattagt aactactacc tttaattttt cgttcgaagc cactgtatct ttgtctttac
    16741 tatttgttga acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta
    16801 ctgctttcca ttttctcatc ataactttct ctccttcttt cttaaaaatt tttgttttgg
    16861 tgagattaga aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc
    16921 tgctatgtta aaactataac caatgaacaa acctagcaat gatgagcacg ctccgactgt
    16981 cgccgaaata aatatcatag tttttaaact gtttgcatat aaatatgctg tagcagctgg
    17041 tgtaattaac attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac
    17101 taaagttaat agaatcatta atagataatg ataaaaactg acattcatcc ccattgcttt
    17161 tgccattaat ggatcaaaag aagtgattaa taattcttta aaaaatagaa caactgtaac
    17221 caacactaaa acagaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat
    17281 atttccaaat aagatatgaa ataaatccgt tgagctattt gcaactccta taaggataac
    17341 tcctaatgct aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact
    17401 attgcttttt ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat
    17461 aaaaaaatta atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat
    17521 agcatcaccc ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac
    17581 aatccctata actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc
    17641 attaataaaa ttttgaagca tattattccc cttgtatatt agagttaggg ttttaacctt
    17701 ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat taactcattt
    17761 cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct ctccaataaa
    17821 tatagtatct ccataggctt tttttaaatt ctcttcatta aatacagatt ctacacttcc
    17881 gtgagcaatt agatttcgat tcaaaagaat aatattatca aaatacttct ttaccttgct
    17941 taaatcatga tgcacaatta gcactgtttt tccctgatgt ttcaattctt ttaacgtatc
    18001 cataattatt cgttcactaa ctaagtctat ccctacaaat ggttcatcta aaaatatgaa
    18061 atccgcattt tgagcaagac atcttgctaa aagtacacgt tgaaattgtc ctccagataa
    18121 ttctcctatt tgatgattag aatattttcc catatcgacc ttttccaaag cctttgaaac
    18181 tctttgccat tctgcatttt taattctttg aaaaactttc attccggcat atgttcccat
    18241 agatacacat tcttttactt ttattggaaa agtaaaatca atatcagctt tttgctctac
    18301 atatgcaatc ttcaccagtt ttttattgat atcttcatta ttaatggaga cctctccctg
    18361 atgagggata atatttaaca ttgctttaag caatgtagat tttccagcac catttggacc
    18421 aataatacct gttaaagcac cttcttgtat ttttaaagaa atctcgttca atgctaaaaa
    18481 atcgttataa caaacattaa gctttttcac ttctaacata actttctcct tctaaatgat
    18541 aatgattatc acgatcatat ggtatacaaa aattatattt tagtcaaccc taaattttat
    18601 aatttagatt tttttctgtt gttgaagatt aggatgtcaa aattatttga aactactcgt
    18661 atagtaaata gtaagtaatt ttcaaaacac gaagaaaaac aaaaagaaaa aaggaaacgc
    18721 cgaagcattt cctttcagtg atttaatgag aacaacacga tttgacttag agtctcaaaa
    18781 agtcgaatag gtacactcat ttaagtgtct ccattcgact ttttggggat gagctggcta
    18841 ttgaggtccg cttaccctat tgaataaata caagattggg tatttctaat aagtccgccc
    18901 ttcatattgg ggtgatcggg gcgaacatgc catcttttca tataccacct cctcgacact
    18961 caataatcaa agattctcgt taatcttctt cgatgcttgc aatgatgttt tcaatattca
    19021 tataacgaaa atcttcttgt ttgatataaa cgatctgagc ggtacatttt tcctttttga
    19081 tgatttcttt tactttttcc tcagaacgtg cgacctgcgg cgttaaaagt aaaacgtcat
    19141 agtctttata attcagtttt tcttcaacgt ttcggatcgt tgcataatct atctcatacg
    19201 atagattttt ggctctgatc gtttcaagca tttttgatac gagcagcgac gcactgattc
    19261 cggtctcgca aataatcaat agatccttat gcataatacc ctcctgtttt tagcctgcaa
    19321 agccaattgg tgaaaatgcc acataagtta aaataacaat gactaccaat gctatagcag
    19381 tcaccttggt gtatttccaa ggctcgatat cgacctttgt atcgaatgag ttcaacacaa
    19441 attctccatc aggtttcaat ttggaaacca ccatcaaaat gataatgtcc agaacgaata
    19501 atgccgtcaa aacataaagg aaatggatat tttggaaaag catttttgat agtgcataca
    19561 agacgatatg cacggcaatc gtcgtttttg ccccaaaagc agttgctttt tttgaataaa
    19621 aggcaaagag aatgatcacg agcagcggca tgttgtatag tccattgaat tcttgaacga
    19681 actgatacaa tccagccggt gcaaaagaaa tgaatggcgc tacgataatg gagataatcc
    19741 ccacaacgac cgttactgtt ttaccagcac gagcgatttg cttatcattt gctttcttat
    19801 tgataagcgg tttataaaaa tccagtgaaa acaaggtcga tgtcgcgttc aaagctcccg
    19861 caaatgatga taagatcgcg ccaaagataa cagcggcgaa aataccaaag atcattttag
    19921 gcaatacttc actgactaaa aaaggatacg cgttatctgg atttgacatt aaactatcgc
    19981 caaaaatatt gcgagcgacg atccctggaa agacaaggaa taacgcacct aagattttga
    20041 agacgccgac taggaaagcg cccttttgtg cctcagctaa attcttgccg gacaatgctt
    20101 tttggacgat catttgattg gtacaccaga aatacagatt gttaaacagc atccctgtaa
    20161 atagtgtcgg ccatggaaca acagccgagt cgatcgatcc cacagagtta agcagccatg
    20221 gcgtgtttac cacaacgtgt tcgatcccgc caacaaagct gccgtcacca agcttcatta
    20281 agcctaaaat tgggaccagc agaccacaag ccagcagacc gactccataa atggagtcac
    20341 tattggcact taacgacaag ccgccgatca gcagatagag gatgccgaca ataccaattg
    20401 ccatcgctac aattatgatc gcgacgatcg gctggacgcc cagcagctca tcaatatgga
    20461 agatcttatt gaacaccagc gatccagaat aaagaaccac gggtaagaaa gaagtcatat
    20521 aggtcacaat aaataagatc gaaataattc gtttggtaac cgtatcataa cgaatctcga
    20581 tgaaatctga gattgtattg atgccgtatt taaaatattt tggcaagaat atcacagcca
    20641 aagctacgat cgcaatcgta gcagttactt cccaggccat tacttccatc cctgcatgat
    20701 aactttgacc attttgtccg actatttgtt cggtggacag gttcgtcatg atgatcgtgc
    20761 cggcgattgg caaagcagtt aagctccgcc ctcccatgaa gaacccttct gatccagaag
    20821 tatcgacact tctggatcta gaataggcaa ataaccatac acagatgaca atcactagga
    20881 aactgattat agaaaaaaga ttcatcgtct agctcctttt caatagatta aagaccggtg
    20941 acctctcgaa tgtagcttct ggctttgact gcatattcaa atggattggc tttagccggg
    21001 tcctgttctg cttcaactac gatccagcct tcatagtcgc tggcatggat tgctgtccag
    21061 attggagtga aatcaatcat cccatcacca ggaaccgtga agacgccttt cttcacgccg
    21121 tttaagaagc tgtctttttc cttttctact tcttcagccg tttcttgacg aatatctttg
    21181 aaatggatgt gtttgatacg atcttgatga cggttgtaaa ccgcgattgg attttcacca
    21241 gaaaagacca aatgtcccgt atcgaacaat aatttaactt tgtctggatc agtcatttcc
    21301 attaaacgat cgatttcttc cgtcgtttga acgccggtac ccatgtgatg atggtaaacg
    21361 atctccatat ccttttcgtg ggccaaatcc cctaattttt ctaatccttc cgccagtttt
    21421 ttccactctt catcagtgaa aactggttta tcatcaaaga ctggcgctgt cataatccct
    21481 tggatactat gaccctgctc agctactacg atgactttag cgcccatcgc atgtaagaaa
    21541 tcccgatgtg ctttgaaggc ttcggcagtt tcttcaaaag gtttagttgt cagaaacgca
    21601 ctgaaccatg cactggcaac ctttaagccg cgaatattca aatattgttc caacacctct
    21661 ggatcttttg gatatttgtt gccaatctct gttccggtga acccagcaag ggccatttca
    21721 ctgatacatt gttcaaaggt attttcacta ccaagctcgg gcatatcatc attggtccac
    21781 gcgattggtg cgatggctaa ttgaatttgt ttggtcatat tctcacactc ctgcttattt
    21841 ttttagttgt actaaactct tcgtatcgaa tgattcttga caagccaacg cgatcttagt
    21901 tgattgcaat ccatcttctg cattaaccgc tggttgacgt ttttcttgaa tacatgagac
    21961 aaattcttgg gcttcacgaa tgaaggcttc tctgaatcgt tctgggaagt tttgtgaaca
    22021 tggacggatg atcccttgat cgttcatgat cgtcacaaga tttttctcag gcgcttgcgc
    22081 tacccgcagc attcctttgg ttcccatgat ctctgtttcg acatgatagc catgggctgc
    22141 gtttctgccg gcaactagta aagctattgt tttatcgctc aggctcatca ttgcggctcc
    22201 agtttcaagc tcaccagcct gatccaattc tggatacgcc gcatttttcc caatcgccca
    22261 cacttgatcg acttcttggc cggtaaacca gcgaaccaaa tcaatatcat ggatcgccat
    22321 atctaagaac aagccgccgc tgtcgttatt catggcaaat ttgacaaagc tttccaagcc
    22381 ttcgctcgga tcgatcccat aacaacgaat cagtgtgatt tcgccgattt cgccatcttc
    22441 taccattttc ttcgcatact gatacgactc atcatagcgg cgcatgaagc ctaacatgaa
    22501 gacctgttca ggatgctctt cgatgacttt ttgggtcttt ttaatatctt caacttcaag
    22561 gcctaatggt ttttcagtaa agacatgctt gccattttcc atcgccaagc ggacttggtc
    22621 acagtgaaag cctgatggtg aaacaataaa gactgcatcg atcacggaac ttttgaccat
    22681 ttcttcatag ctggaaaaag tatgcttgac gcctaatttc ttctgtgcga atgctaattc
    22741 tgtttcaact aaactgcacg cagccgtaag ctcacaacaa ctgacacctg ttgctaaatt
    22801 ttgtgcatgt acttttccta atcttcctaa accgacgatg cctactctaa tgttgttcat
    22861 gaaatacctc cgttttctga gaattactca ttgtatagtg ctggtcgttc atccatttga
    22921 atcattactt ttgtttttgt ttcacgagac tcatgacaag ccatcatggt gatcgctgct
    22981 acatacccat cccaagcggt aggaccgaca accgcatcat ttttgatcga atcgatccac
    23041 agcttgaatt cataatcata ggcatccaaa aagcgggtct gccaatctgg tgaaacaggt
    23101 gtgtaatttt tttcttccga tttaattcgt gtataggctg ggtccgtcat tcctacttct
    23161 gccgtttcga aaaccacatc acatttaata tcgtagccaa agcggcaatt cacaaagatt
    23221 tccaaatcaa tcgttacacc ggattttgtt tgaaagatca gcagctgcgg atcatgcaaa
    23281 tcggcatggg agttgctggt ttttttcggc aagatcattt gaacagattc ataatcttca
    23341 tttagcaacc agcgaagcac atcaatctca tgaatcgcgg tgttgctgat cgccatcggt
    23401 gttgtgtagt tttcatctgc gctaggattt ctatgggcac aatgcatcat taatgcttct
    23461 ccgtattgct tcgattcgat tgcttctttc agttcaatgt agccgcggtc atagcggcgc
    23521 atgaagccaa cctgaaccaa tttcttccca taagcaactt ccgcatcaac gatccggcgg
    23581 cagcctgctg catccatcgc taatggcttt tcgcagaaaa catatttccc atgcttgatt
    23641 gcttctaaca catactgctc atgggtcggg tcccaagaag tcacaacaac gacatccaca
    23701 tcttcagaag cgatcaatgc ttctccggtt tcaaagaatt tagcatcgac acgtttggca
    23761 acctctttag cagcttttac attgatatct gagactgctg tcacataagc cccttgtgtt
    23821 ttctgattga tcctgtcgat atgctcgatg ccgattgcac ctgttccgat aacgcctact
    23881 tttactctca attacttatc cccctgttct gttgtttaag cacacatagt atgcgcttac
    23941 aaaattattc ctgtgtagct tttattctta aaaggtagag ccctctcttt gctccgcctt
    24001 ttactaccca taagcttgta cgtataattc cttaatttga ctatgactag gcaccttttt
    24061 attattttgc ggacttccgc tggtcaacgc atccgcggcc attttgtcta aggctgccat
    24121 aaacgcttgc tgatcaatac cataatctcg aatcgttggg atcgacagct gctcacagag
    24181 ctcttggatt tttttaatca gataatccgc agcctcttcc agcgataaat cttcggctgc
    24241 cggataaata aatctggcga tcgtcgctaa ctcagtctga atagcatcct ttgagaattc
    24301 aaaaaagacc ggcaataaca tcgcatttga tatcccatga ggaatgtgaa aaagtgcccc
    24361 caccggtcga ctcattccat gaatcaatgt gactgacgca ttcgaaaagg caatccccgc
    24421 ctccgttgaa gctaagctca tttgttctct ggcttcagtg tcgtcgctat tttcatatgc
    24481 ctgagaaagc gtgttcaaaa ttttcttcat cgcttccagt gaaaatagtt gcgttacttc
    24541 attggctttg actgaaacat aagattccaa agcgtgacat agcgcatcga ctcccgttgc
    24601 cgcggttact tttggtggta cagttttcgt aaaatcagag tcaacgattg agatctgtgg
    24661 catgaaaacg agatccttca tcatcatttt gactgctgtt tttgtattgg taataactgc
    24721 cacatcggtt acttccgaac cagttccagc agttgttgac actgcaatca gcggaatgcc
    24781 cgcttgcagc tgatcaaaat ttcttgcatg aatcgcttcg agacttaatt gattagcagc
    24841 caaaacggct accaccttgg ctgtatccag acagctgccg ccaccaaaag caatgatatg
    24901 atcacattga tgcttctgaa aaagcttcag cgcatcataa acattttctt gcgtcggttc
    24961 ggcttgaatg tctgtatact ccgctaattg atcatctgtt tttcccaaca gctccttgat
    25021 cttttgaaga tgcccggtct gttccataac cgtatcacta atgattaacg gcttgttgcc
    25081 tagttgttct gattcttcca taagaaattg gattgttccg ccgccaattc ttaaattggc
    25141 aggcattttc agttgaatat tcacagaaaa aacctctctc tggcttatag cctataacag
    25201 aatcctcgaa gctctttaac agagcttcga agcaaatctt taaatctcac tttgcgcata
    25261 acgtgctgta acaactttcc gatgggtata aaactcaaca ctgtctttgc cattggcatg
    25321 caaatcacca aagaaagagt gcttccagcc tgaaaaggca aaccaagcca tcggtgcagg
    25381 caccccaaga ttgaccccca gcatcccagc atcaacattt tctctgaagt agcgaatcgc
    25441 tgatgcgttg ttggtataca gacacgaacc attcgcaaat tcagattcat tcgtcagctc
    25501 aacggctgcc atcaaatctt ttacccgcac gattgatgcg accggggcaa aaatctcatc
    25561 ctgccagatc ttcatatcca tcgtgacatt atcaaaaatc gttggtccaa caaaatagcc
    25621 tttttctgga atgttttcgc gaccatctag taccagtttc gctccttcat cgaccccttt
    25681 ttggatatag tcaaaggtcc gctgctgtgc atcttttcga atcactggtc ctaaaaagac
    25741 cccttcgtcc aagccattgc cgatcttgat cgcttttgcc gcagccttaa atttttcaac
    25801 aaactgatcc gcgatttcct cttccaaaac aatgacggag gctgccatgc agcgttcacc
    25861 agcagaacca aaagctccgc caataaggtc cttgactgcc cgatcaatat tggcatcttt
    25921 cagcacaaca acatgatttt tcgctccagt caacgcttgt acccgtttta gattttttgt
    25981 tccttcacgg taaacgtatt caccgacatt ttttgatcct acaaagctga tgccttttac
    26041 cagtggatct tgcagcaatg tgttgacaac atcgtgggca ccatagacaa cattaaaaac
    26101 accgctagga aagcctgctt tttcaaccag ctcaacaatt ttctccatca acaatggtgt
    26161 tttttctgaa ggcttcagca ccaccgtatt tcctgccgcc agagccatcg ggaagatcca
    26221 gaagggcacc atcattggaa aattaaacgg tgtgatccct ccgattactc cgattgggta
    26281 acgataattc gtgatctcaa tatcagtcgc cgctgttgat aaagaatctc ccatcattaa
    26341 attggtgatt cccgctgcgt gctccacatt ttcgattcct cgttgaactt ctcccagtga
    26401 ctcggacaat gatttcccat tttccagcgt gatcaaacgc gccaattcac ttttatgatc
    26461 tgttaaaagc tgatgcagct tgaacagata acgtccgcga acaggaacag gaactttttt
    26521 ccaagtttga aacgcagcat cggcagcttt aactgcttcg gctgtctctg ctttgctaga
    26581 caacggtaca ttcgccagcg cttcttctgt agccggattc attacttctt caaactctgt
    26641 tgagcgacta gcgacccaac gaccatcaat ataatttctt aaaaatcttg tcattt
//
LOCUS       DADQRX010000031        26656 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.031, whole genome shotgun sequence.
ACCESSION   DADQRX010000031 DADQRX010000000
VERSION     DADQRX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.031"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..516
                     /locus_tag="J8V14_001219"
     CDS             172..516
                     /locus_tag="J8V14_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA0921336.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="J8V14_001220"
                     /pseudo
     CDS             517..936
                     /locus_tag="J8V14_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="J8V14_001221"
     CDS             complement(1028..1378)
                     /locus_tag="J8V14_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921337.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="J8V14_001222"
     CDS             complement(1344..2675)
                     /locus_tag="J8V14_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBA0921338.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="J8V14_001223"
     CDS             2931..3497
                     /locus_tag="J8V14_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0921339.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="J8V14_001224"
     tRNA            3642..3729
                     /locus_tag="J8V14_001224"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="J8V14_001225"
     CDS             4063..5037
                     /locus_tag="J8V14_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HBA0921340.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="J8V14_001226"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="J8V14_001226"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBA0921341.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="J8V14_001227"
     CDS             complement(7452..7898)
                     /locus_tag="J8V14_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBA0921342.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="J8V14_001228"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="J8V14_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBA0921343.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="J8V14_001229"
     CDS             8363..8881
                     /locus_tag="J8V14_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBA0921344.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="J8V14_001230"
     CDS             complement(8958..9914)
                     /locus_tag="J8V14_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0921345.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="J8V14_001231"
     CDS             10080..10886
                     /locus_tag="J8V14_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921346.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="J8V14_001232"
     CDS             10883..11872
                     /locus_tag="J8V14_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA0921347.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="J8V14_001233"
     CDS             11869..12873
                     /locus_tag="J8V14_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA0921348.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="J8V14_001234"
     CDS             13005..13547
                     /locus_tag="J8V14_001234"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBA0921349.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="J8V14_001235"
     CDS             13567..14265
                     /locus_tag="J8V14_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBA0921350.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="J8V14_001236"
     CDS             14286..14498
                     /locus_tag="J8V14_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBA0921351.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="J8V14_001237"
     CDS             14498..15460
                     /locus_tag="J8V14_001237"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBA0921352.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="J8V14_001238"
     CDS             15478..15873
                     /locus_tag="J8V14_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBA0921353.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="J8V14_001239"
     CDS             16036..16401
                     /locus_tag="J8V14_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBA0921354.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="J8V14_001240"
     CDS             16398..18209
                     /locus_tag="J8V14_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBA0921355.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="J8V14_001241"
     CDS             complement(18271..18438)
                     /locus_tag="J8V14_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBA0921356.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="J8V14_001242"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="J8V14_001242"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBA0921357.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="J8V14_001243"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="J8V14_001243"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBA0921358.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="J8V14_001244"
     CDS             21195..22175
                     /locus_tag="J8V14_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA0921359.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22264..23088
                     /gene="tsaB"
                     /locus_tag="J8V14_001245"
     CDS             22264..23088
                     /gene="tsaB"
                     /locus_tag="J8V14_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBA0921360.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="J8V14_001246"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="J8V14_001246"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBA0921361.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="J8V14_001247"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="J8V14_001247"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBA0921362.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="J8V14_001248"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="J8V14_001248"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBA0921363.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="J8V14_001249"
     CDS             complement(25707..26408)
                     /locus_tag="J8V14_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBA0921364.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8636 a         4551 c         5543 g         7926 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaacat accaacatta gaaacaagtt ttaatacttt acaaatataa
    26521 tttgttatta tgtaaaaaat atacaatggt attgaataat ttgttttgaa gcgaatgatt
    26581 tgtgtataaa aaacaacaat aatacatata gtaaacgcta acaacgataa tataatttat
    26641 ttttttaatt tattca
//
LOCUS       DADQRX010000032        26608 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.032, whole genome shotgun sequence.
ACCESSION   DADQRX010000032 DADQRX010000000
VERSION     DADQRX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.032"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..384)
                     /locus_tag="J8V14_001250"
     CDS             complement(<1..384)
                     /locus_tag="J8V14_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921365.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="J8V14_001251"
     CDS             complement(569..967)
                     /locus_tag="J8V14_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBA0921366.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="J8V14_001252"
     CDS             complement(964..1737)
                     /locus_tag="J8V14_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBA0921367.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="J8V14_001253"
     CDS             complement(1734..2417)
                     /locus_tag="J8V14_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBA0921368.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="J8V14_001254"
     CDS             complement(2584..3471)
                     /locus_tag="J8V14_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921369.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="J8V14_001255"
     CDS             complement(3468..4337)
                     /locus_tag="J8V14_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921370.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="J8V14_001256"
     CDS             complement(4474..5760)
                     /locus_tag="J8V14_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0921371.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="J8V14_001257"
     CDS             6033..6611
                     /locus_tag="J8V14_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBA0921372.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="J8V14_001258"
     CDS             6611..7540
                     /locus_tag="J8V14_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921373.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="J8V14_001259"
     CDS             7537..9273
                     /locus_tag="J8V14_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA0921374.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="J8V14_001260"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="J8V14_001260"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBA0921375.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="J8V14_001261"
     CDS             complement(10169..10678)
                     /locus_tag="J8V14_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBA0921376.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="J8V14_001262"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="J8V14_001262"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBA0921377.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="J8V14_001263"
     CDS             complement(11979..13070)
                     /locus_tag="J8V14_001263"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBA0921378.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="J8V14_001264"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="J8V14_001264"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBA0921379.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="J8V14_001265"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="J8V14_001265"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBA0921380.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="J8V14_001266"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="J8V14_001266"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBA0921381.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="J8V14_001267"
     CDS             complement(17033..18007)
                     /locus_tag="J8V14_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBA0921382.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="J8V14_001268"
     CDS             complement(17988..19871)
                     /locus_tag="J8V14_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBA0921383.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="J8V14_001269"
     CDS             complement(19889..22114)
                     /locus_tag="J8V14_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBA0921384.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="J8V14_001270"
     CDS             22229..23263
                     /locus_tag="J8V14_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0921385.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="J8V14_001271"
     CDS             complement(23330..24343)
                     /locus_tag="J8V14_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0921386.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="J8V14_001272"
     CDS             complement(24421..24609)
                     /locus_tag="J8V14_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBA0921387.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="J8V14_001273"
     CDS             complement(25049..25177)
                     /locus_tag="J8V14_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBA0921388.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="J8V14_001274"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="J8V14_001274"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBA0921389.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
BASE COUNT         7604 a         5756 c         4724 g         8524 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagc
//
LOCUS       DADQRX010000033        26543 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.033, whole genome shotgun sequence.
ACCESSION   DADQRX010000033 DADQRX010000000
VERSION     DADQRX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.033"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(89..1219)
                     /locus_tag="J8V14_001275"
     CDS             complement(89..1219)
                     /locus_tag="J8V14_001275"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBA0921390.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1868..3070)
                     /locus_tag="J8V14_001276"
     CDS             complement(1868..3070)
                     /locus_tag="J8V14_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0921391.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="J8V14_001277"
     CDS             complement(3130..3333)
                     /locus_tag="J8V14_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBA0921392.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4707)
                     /locus_tag="J8V14_001278"
                     /pseudo
     CDS             complement(3475..4707)
                     /locus_tag="J8V14_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4870..5682
                     /locus_tag="J8V14_001279"
     CDS             4870..5682
                     /locus_tag="J8V14_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921393.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6706..6858)
                     /locus_tag="J8V14_001280"
     CDS             complement(6706..6858)
                     /locus_tag="J8V14_001280"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921394.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7049..7927)
                     /locus_tag="J8V14_001281"
     CDS             complement(7049..7927)
                     /locus_tag="J8V14_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0921395.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7966..9084)
                     /locus_tag="J8V14_001282"
     CDS             complement(7966..9084)
                     /locus_tag="J8V14_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBA0921396.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9081..11180)
                     /locus_tag="J8V14_001283"
     CDS             complement(9081..11180)
                     /locus_tag="J8V14_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBA0921397.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11231..12052)
                     /locus_tag="J8V14_001284"
     CDS             complement(11231..12052)
                     /locus_tag="J8V14_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA0921398.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12049..12858)
                     /locus_tag="J8V14_001285"
     CDS             complement(12049..12858)
                     /locus_tag="J8V14_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0921399.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12879..13373)
                     /locus_tag="J8V14_001286"
     CDS             complement(12879..13373)
                     /locus_tag="J8V14_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0921400.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13385..13873)
                     /locus_tag="J8V14_001287"
     CDS             complement(13385..13873)
                     /locus_tag="J8V14_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA0921401.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13950..16646)
                     /locus_tag="J8V14_001288"
     CDS             complement(13950..16646)
                     /locus_tag="J8V14_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0921402.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17074..18147)
                     /locus_tag="J8V14_001289"
     CDS             complement(17074..18147)
                     /locus_tag="J8V14_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0921403.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18144..18971)
                     /locus_tag="J8V14_001290"
     CDS             complement(18144..18971)
                     /locus_tag="J8V14_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921404.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18972..19778)
                     /locus_tag="J8V14_001291"
     CDS             complement(18972..19778)
                     /locus_tag="J8V14_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921405.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19790..20875)
                     /locus_tag="J8V14_001292"
     CDS             complement(19790..20875)
                     /locus_tag="J8V14_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921406.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20892..21434)
                     /locus_tag="J8V14_001293"
     CDS             complement(20892..21434)
                     /locus_tag="J8V14_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921407.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21692..22681
                     /locus_tag="J8V14_001294"
     CDS             21692..22681
                     /locus_tag="J8V14_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA0921408.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22678..23415
                     /locus_tag="J8V14_001295"
     CDS             22678..23415
                     /locus_tag="J8V14_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBA0921409.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23412..23963
                     /gene="coaC"
                     /locus_tag="J8V14_001296"
     CDS             23412..23963
                     /gene="coaC"
                     /locus_tag="J8V14_001296"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBA0921410.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23979..24611
                     /locus_tag="J8V14_001297"
     CDS             23979..24611
                     /locus_tag="J8V14_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA0921411.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24696..25343
                     /locus_tag="J8V14_001298"
     CDS             24696..25343
                     /locus_tag="J8V14_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0921412.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25348..26331
                     /locus_tag="J8V14_001299"
     CDS             25348..26331
                     /locus_tag="J8V14_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBA0921413.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8113 a         5607 c         4502 g         8321 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat ttccattttt
     6181 gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg aaaaattttt
     6241 gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct caaattattt
     6301 ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt cctaataaag
     6361 aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt tcttattttt
     6421 cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc aatcatttca
     6481 aggtaatata actacctgag tttattatcg attaaaatgc taactacatt tgcaagagaa
     6541 gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat aggaataaaa
     6601 tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata atcacaactc
     6661 tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc aaaaaaattc
     6721 aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac attggtaagc
     6781 aactataact attaccacag acataaagta acacaatata ttactattta agtgtacgcc
     6841 accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag
     6901 catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct ttttatttga
     6961 attcattttc gctttatcca acttcatttg atcatttgat tactcggtta aatacttttg
     7021 acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc gcttttctgg
     7081 ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta ataatgtagc
     7141 tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta tcctgaactt
     7201 ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta tctggcgaaa
     7261 acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg
     7321 aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc gctggtcctg
     7381 ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg
     7441 gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc acgaaagtcc
     7501 cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca acacctgcta
     7561 accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca tctgttgtcc
     7621 attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa ttttgccaag
     7681 caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga atatcaattg
     7741 gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta aaaaaatcaa
     7801 tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt tccaaaatcg
     7861 ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca attgccccat
     7921 atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg
     7981 ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat accagtaagc
     8041 gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg
     8101 taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc aacgataaag
     8161 accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc
     8221 aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa aggctgttga
     8281 atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc acgccccgcc
     8341 aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc catcaatgta
     8401 gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg caaggtaagt
     8461 acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct cctgtaattt
     8521 ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt ccaattcgtc
     8581 caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat gtaaattctc
     8641 gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa agccccctgt
     8701 aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc cattacaaaa
     8761 gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat aaattcttaa
     8821 gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg tcatccacat
     8881 atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac cagaagtcag
     8941 cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt
     9001 acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt ttttaaattg
     9061 ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt
     9121 tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga caaatttgaa
     9181 atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa cttcaaattc
     9241 ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt tttgaatttc
     9301 aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca gccgcatttg
     9361 ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata cggccaccac
     9421 caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt tacgaaagct
     9481 tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt ggcgccggat
     9541 tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa agaactagca
     9601 gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg ataaaaaggc
     9661 atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt tccatccaca
     9721 atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata gtgcttcact
     9781 gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc aaaaggacca
     9841 tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt tttgtatcgc
     9901 ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat acaggccttc
     9961 caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc tgataaccaa
    10021 acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc ctcaataatt
    10081 cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc cccagtatgt
    10141 tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga atgattgata
    10201 cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca tagaaccgtt
    10261 ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata catatcctta
    10321 ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac ctgtccctgt
    10381 cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag atggacaact
    10441 ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa tgtttccttc
    10501 agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc
    10561 acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt
    10621 tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg cacatctttc
    10681 actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg aacaagcata
    10741 cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt aaacgtcgaa
    10801 taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat acgatgatcc
    10861 gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa cgaattggtt
    10921 tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa attttctcga
    10981 taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta
    11041 cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct gtattcgttt
    11101 gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt gggtgaataa
    11161 attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc ctagtagctg
    11221 cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg accattccaa
    11281 aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc aatgttacca
    11341 ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc attagttctt
    11401 cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc gtcattgcac
    11461 ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt aacccgttcg
    11521 catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg ccatagtatc
    11581 gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc aaaatatttc
    11641 cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact ccccagaaca
    11701 atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg ttgatacttt
    11761 ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca atccccatga
    11821 caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag
    11881 ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa taggcgaaac
    11941 caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag cgccaaaaca
    12001 tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc attgaaagaa
    12061 atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac
    12121 agatatattt tttagttgta gatctctttg ggctataaca attgcaataa tcgttccaac
    12181 tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc caaagaaaaa
    12241 ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc caactgccgg
    12301 cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat tatccaagac
    12361 gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag cataaagcgc
    12421 atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat caccagattt
    12481 tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa tcatattcat
    12541 taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat ccacgtttcc
    12601 tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct ccgccgtact
    12661 agagattccg atattgacga cacccataaa gatggcttct agtgacgccc cgacaatcaa
    12721 ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa tcggacgact
    12781 cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac
    12841 tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact caaaatagag
    12901 atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg ataaagttca
    12961 actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt tgataaggtt
    13021 ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat taattcgatt
    13081 tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac taaaaccatc
    13141 aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc aactgtttta
    13201 ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg aatatcgttg
    13261 gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt tgtccaagtc
    13321 atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat catttttctt
    13381 cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga atggtcatct
    13441 ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt aattccttgg
    13501 ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata gttacagcaa
    13561 tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca aataaatcgg
    13621 taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc aactcctgtt
    13681 caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc ttccccgtca
    13741 gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct attagaattt
    13801 tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt cctgtgccaa
    13861 cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa
    13921 aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc taataattca
    13981 tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc cattgattga
    14041 aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc ataattcggt
    14101 tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc cgtatgcata
    14161 tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc atatttagaa
    14221 atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa acggtccgca
    14281 attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa ttctgccggc
    14341 ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc taagatgtca
    14401 taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat
    14461 tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag ttctctgtag
    14521 tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac atctttcaat
    14581 ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact ttgagaaaag
    14641 ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc aatctgttta
    14701 aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt cgtcaaatta
    14761 ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa
    14821 gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt cacgaaattc
    14881 cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa cgcttcaaaa
    14941 acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt actatctccg
    15001 tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga taaagtcacg
    15061 acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa atgctccaag
    15121 aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg atttatcccg
    15181 gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat ttctgcaaca
    15241 ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac ctttctaaac
    15301 atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat gtgatgcacc
    15361 aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc ttcatttatc
    15421 agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg atccgttttt
    15481 atcttttgat ttttctttat caataaacta gcaatcggca agctttctac ttcggattcc
    15541 agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt
    15601 cgactataca catccgcaca actgatttta atgatattct tcagcttacc aatattccca
    15661 ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat atctttttgt
    15721 aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg
    15781 cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa ggtgtctaat
    15841 aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa
    15901 tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa gagcttttct
    15961 tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt gttggccgtt
    16021 gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt gtatccaaac
    16081 agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa gatagcaaat
    16141 ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa
    16201 aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt aggcggatat
    16261 ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg accaactaca
    16321 gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc atctaaaggc
    16381 ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac taatctcgtc
    16441 aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt ctcggcagtg
    16501 acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc tttagcgctc
    16561 tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat cggctgcaat
    16621 atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt cgaatatttg
    16681 cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa tatgattttt
    16741 atgttttttc atagctacaa atattgaact ggattcatat attagtttta ttaatagatt
    16801 acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg taactttttc
    16861 aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt ccacttttct
    16921 ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg tttcatccgc
    16981 ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct ctttagaacg
    17041 ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct tgaattctaa
    17101 gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga cttcaagatg
    17161 ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt ttggcaataa
    17221 cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt gtgccgcatt
    17281 ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt tcacggtttt
    17341 tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca cataatgtaa
    17401 atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg ccacggaggc
    17461 ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga catttggtgt
    17521 taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac tattcaaaga
    17581 atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa tcagcatcaa
    17641 actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat ttgcatcaaa
    17701 gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg aatactgatt
    17761 atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc ctttgatctt
    17821 cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca tatattcact
    17881 tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca tcgcttcatt
    17941 agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt tcgtgatcaa
    18001 actaggatcg atataatctc cccagttata gatggtaagt acctttgctc cactcatgcc
    18061 acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga tgagaatacc
    18121 taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg tactcttctg
    18181 tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc aatgaccaat
    18241 atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact gattccttgt
    18301 cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt gacgaaaaat
    18361 gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc tatgattcct
    18421 ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc accaaggtct
    18481 ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac catcaagacg
    18541 acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc taatccgaat
    18601 cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg agagactaat
    18661 aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt gtaatagatt
    18721 cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa agccagcatg
    18781 aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc ataattttcc
    18841 caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata aaaaatcaga
    18901 tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata cgaccattta
    18961 aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag catcacgatg
    19021 aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt ttgtgtaacc
    19081 aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc aatcagacga
    19141 gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact tttcacacca
    19201 tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc gcctaaatct
    19261 ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat tggcataatc
    19321 ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt gaataatatc
    19381 tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg cacactgaaa
    19441 atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa aataatcagc
    19501 atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg gtaactgatg
    19561 atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt catagaaagc
    19621 gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc attcatatca
    19681 aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc gagccacaaa
    19741 atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc tattcttcgt
    19801 agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg acatggatat
    19861 cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc gttgaatgga
    19921 ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca ccacgaaaca
    19981 gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc gtcaaatcct
    20041 ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg acacattcaa
    20101 attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc acgatattgc
    20161 tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata tctacgggtg
    20221 taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc attgccaacg
    20281 cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt tgcagatccc
    20341 gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac ggttcatcta
    20401 atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc tgtctttgac
    20461 caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc atacgcaaag
    20521 catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc aatccaaaag
    20581 caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag actgtattta
    20641 cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag atgtcccctt
    20701 ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca cagccggaag
    20761 gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt tttaatactg
    20821 tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc cccacgtttt
    20881 taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt tcgttttttg
    20941 agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg tcctgctttt
    21001 gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac aaaaataaac
    21061 gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa agtcagcaag
    21121 acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc atacccgttt
    21181 tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag attcgctgct
    21241 tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga aaagaatgtt
    21301 tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt tgacaaatct
    21361 gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt tcttaacttc
    21421 tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact tcctgtttac
    21481 taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta aaattttttt
    21541 tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt tatttttcac
    21601 ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt atggtatgat
    21661 aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt ttcaggtaat
    21721 aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt tggctgaaaa
    21781 tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat tggcaatgag
    21841 cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt tatccggaac
    21901 tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg ctactggttt
    21961 tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac caggagactg
    22021 gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg gtacagcagg
    22081 gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct ccgataaaaa
    22141 agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc tgttacataa
    22201 actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca catggctaaa
    22261 aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga caacaaaagc
    22321 tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc agctaagcag
    22381 tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg ctgggggaat
    22441 caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga ttggaattga
    22501 ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg cggatttgca
    22561 gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg aaacagcatc
    22621 taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg accataaatg
    22681 aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg tgctattaca
    22741 aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc caatccggat
    22801 accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca ctccaatata
    22861 acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga actaacgaaa
    22921 aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac tcctgctttt
    22981 agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca agaagaactg
    23041 gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat ctcttctaac
    23101 accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta tctacgtgaa
    23161 tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc aaaggaatct
    23221 cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat tttcgcaaat
    23281 gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa agatggaaca
    23341 gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa aattagactg
    23401 gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct gcttacaaaa
    23461 gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg attatgtcta
    23521 aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag cgcgcagtcc
    23581 atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa ctagcaaaac
    23641 aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag ctagcaaacg
    23701 gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga acaccaaaat
    23761 tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag cgtaatctgg
    23821 aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt ttagcttgtg
    23881 gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag gtcatgctga
    23941 ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca aaaaacttta
    24001 cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct ttaggtttta
    24061 tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt gcttcgatcg
    24121 ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc agcatgatcc
    24181 gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc ccagtcatcg
    24241 gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc atcttgattg
    24301 gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa aaagcagatt
    24361 ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt ttagtgatga
    24421 atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca aatttaaatg
    24481 ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa ggaatcgtcg
    24541 caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag cacccttctc
    24601 ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg tgttactatt
    24661 gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt gtaggtgcaa
    24721 acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat gaaatttttg
    24781 caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt tcgtatgtct
    24841 cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc atcgatgtat
    24901 tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg gatggcgcaa
    24961 tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg gtcagtgctg
    25021 tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat attactaaac
    25081 attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt caaccagtca
    25141 cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat gagaaggcat
    25201 caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg gaagaaacgg
    25261 atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat acagcattcc
    25321 aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca tcctggttct
    25381 gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg cgaattactg
    25441 atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga atcagtctct
    25501 ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt tgagaataaa
    25561 agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa tctaggtggt
    25621 tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga tacgctagac
    25681 tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac actttactgg
    25741 cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag tggtgtggga
    25801 acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt tactgcagga
    25861 tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat caactataaa
    25921 aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt cgatgttatt
    25981 ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc cgttgatgga
    26041 cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga ccttggtgta
    26101 ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag tgatcagtat
    26161 aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga aaatggcaag
    26221 attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc gcatgaatat
    26281 atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata aaatcatgct
    26341 gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag aaattcacga
    26401 aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt cttctgcttc
    26461 cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc
    26521 tttttccgaa taaacggtgt tca
//
LOCUS       DADQRX010000034        26058 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.034, whole genome shotgun sequence.
ACCESSION   DADQRX010000034 DADQRX010000000
VERSION     DADQRX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.034"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..260)
                     /locus_tag="J8V14_001300"
     CDS             complement(<1..260)
                     /locus_tag="J8V14_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBA0921414.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQK"
     gene            complement(351..803)
                     /locus_tag="J8V14_001301"
     CDS             complement(351..803)
                     /locus_tag="J8V14_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBA0921415.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(816..1226)
                     /locus_tag="J8V14_001302"
     CDS             complement(816..1226)
                     /locus_tag="J8V14_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921416.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1501..1713
                     /locus_tag="J8V14_001303"
     CDS             1501..1713
                     /locus_tag="J8V14_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921417.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1716..1928
                     /locus_tag="J8V14_001304"
     CDS             1716..1928
                     /locus_tag="J8V14_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921418.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(2220..2444)
                     /locus_tag="J8V14_001305"
     CDS             complement(2220..2444)
                     /locus_tag="J8V14_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBA0921419.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2522..2710
                     /locus_tag="J8V14_001306"
     CDS             2522..2710
                     /locus_tag="J8V14_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921420.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            3036..3371
                     /locus_tag="J8V14_001307"
     CDS             3036..3371
                     /locus_tag="J8V14_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921421.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3604..4545
                     /locus_tag="J8V14_001308"
     CDS             3604..4545
                     /locus_tag="J8V14_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HBA0921422.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4547..5437
                     /locus_tag="J8V14_001309"
     CDS             4547..5437
                     /locus_tag="J8V14_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBA0921423.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5480..6280
                     /locus_tag="J8V14_001310"
     CDS             5480..6280
                     /locus_tag="J8V14_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBA0921424.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6295..7146
                     /locus_tag="J8V14_001311"
     CDS             6295..7146
                     /locus_tag="J8V14_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBA0921425.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7347..7808
                     /locus_tag="J8V14_001312"
     CDS             7347..7808
                     /locus_tag="J8V14_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0921426.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7834..8286
                     /locus_tag="J8V14_001313"
     CDS             7834..8286
                     /locus_tag="J8V14_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921427.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8392..8586
                     /locus_tag="J8V14_001314"
     CDS             8392..8586
                     /locus_tag="J8V14_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921428.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8617..8910
                     /locus_tag="J8V14_001315"
     CDS             8617..8910
                     /locus_tag="J8V14_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921429.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8940..9380
                     /locus_tag="J8V14_001316"
     CDS             8940..9380
                     /locus_tag="J8V14_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0921430.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9455..9922
                     /locus_tag="J8V14_001317"
     CDS             9455..9922
                     /locus_tag="J8V14_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBA0921431.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10669..10899
                     /locus_tag="J8V14_001318"
     CDS             10669..10899
                     /locus_tag="J8V14_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921432.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11058..11891
                     /locus_tag="J8V14_001319"
     CDS             11058..11891
                     /locus_tag="J8V14_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBA0921433.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11884..13299
                     /locus_tag="J8V14_001320"
     CDS             11884..13299
                     /locus_tag="J8V14_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBA0921434.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13311..14840
                     /locus_tag="J8V14_001321"
     CDS             13311..14840
                     /locus_tag="J8V14_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA0921435.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14932..15810
                     /locus_tag="J8V14_001322"
     CDS             14932..15810
                     /locus_tag="J8V14_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HBA0921436.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15812..16129
                     /locus_tag="J8V14_001323"
     CDS             15812..16129
                     /locus_tag="J8V14_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921437.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16178..16879
                     /locus_tag="J8V14_001324"
     CDS             16178..16879
                     /locus_tag="J8V14_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBA0921438.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16894..17784
                     /locus_tag="J8V14_001325"
     CDS             16894..17784
                     /locus_tag="J8V14_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBA0921439.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17807..18022
                     /locus_tag="J8V14_001326"
     CDS             17807..18022
                     /locus_tag="J8V14_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921440.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18034..18375
                     /locus_tag="J8V14_001327"
     CDS             18034..18375
                     /locus_tag="J8V14_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921441.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18375..18746
                     /locus_tag="J8V14_001328"
     CDS             18375..18746
                     /locus_tag="J8V14_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921442.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18739..19134
                     /locus_tag="J8V14_001329"
     CDS             18739..19134
                     /locus_tag="J8V14_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBA0921443.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19136..19513
                     /locus_tag="J8V14_001330"
     CDS             19136..19513
                     /locus_tag="J8V14_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921444.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19514..20122
                     /locus_tag="J8V14_001331"
     CDS             19514..20122
                     /locus_tag="J8V14_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA0921445.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20122..20463
                     /locus_tag="J8V14_001332"
     CDS             20122..20463
                     /locus_tag="J8V14_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921446.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20705..23494
                     /locus_tag="J8V14_001333"
     CDS             20705..23494
                     /locus_tag="J8V14_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBA0921447.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23484..24224
                     /locus_tag="J8V14_001334"
     CDS             23484..24224
                     /locus_tag="J8V14_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA0921448.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24221..>26058
                     /locus_tag="J8V14_001335"
     CDS             24221..>26058
                     /locus_tag="J8V14_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921449.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9396 a         3732 c         5277 g         7653 t
ORIGIN      
        1 tacttttgat ttatttcatc atttaaatct tctttctctt tttttaataa aataatatca
       61 ttttttaaca tagtaatttg tttatttaaa tctgatagtg tttcttcctt acttaatata
      121 aatctttctc tatcatgtaa tttatttagt ttttcatcat ttttttgttc taattcataa
      181 gcttcttttt ttattgtgtt agctttcgac agtttagtga ttcctataat taataaaata
      241 agtccaatca ataataacat tcaaaactcc tcatttcttt gatataatta ttgtagagtt
      301 tactcagaaa tgaggaaaga gtccatgtga aagcatgggc ttttttattt ttaatgttgt
      361 aatactaaat gttcaagatt atgaggaagc cctaaatatt caataatttg gaactgactc
      421 atatcagata aatcttgttc atctattgtt ctagttaaca tttcaattgc aaacatatta
      481 gcttcagctt ctataccata aatcatacaa ctaaggttgt ttgctctaaa agctgctgtg
      541 caaatacctt tatggagtct aacgtgggct atttcatgcc ataatacaaa tcttttcata
      601 gaaggtgata ggttttgatt aattaatatc acataacatc ttttattgtg tatcgtttgt
      661 cctaatgttt tattccctaa atcagtttca attaaatcta tatcagaata tttgattatt
      721 ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat ttcttctaat
      781 atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt tttaggagtg
      841 aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc atcgtataat
      901 ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc atctgaattc
      961 aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt ttctgttaaa
     1021 tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac attaaagtaa
     1081 tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct agacatgctt
     1141 ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg ttcttttaat
     1201 aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata ccgacaaaaa
     1261 aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat actagttttg
     1321 taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt ctccccagac
     1381 atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag catatttgca
     1441 gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg ggtatgaagc
     1501 atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag tcaaagagag
     1561 ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat cgaaaaaaga
     1621 aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt agaagtagat
     1681 tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg agcaaagcgg
     1741 agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg aaggaattta
     1801 aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata acaattgatg
     1861 tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga gttagagctg
     1921 tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt gctgctctcc
     1981 tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct tggaggtggt
     2041 gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg gattttgaat
     2101 gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt agcagaagtt
     2161 acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc taaatatgat
     2221 taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat ttttccatct
     2281 ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc gatttttact
     2341 gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc tttgacattc
     2401 caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt cacctccatt
     2461 tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa ggagaagtga
     2521 tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac aacgcaggaa
     2581 acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag ataggcaagc
     2641 tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa atagggagat
     2701 tagattatga agaagacaga cacacttttt ataggattca ttttggggtt attagtgatt
     2761 gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt tttaatcaat
     2821 ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa aaatcggtaa
     2881 agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca agcaacatat
     2941 cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag caaagagtca
     3001 catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt gaaaatctta
     3061 tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt acagcccaaa
     3121 aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg
     3181 ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa
     3241 ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata
     3301 cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg
     3361 aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt
     3421 gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct
     3481 ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact
     3541 atttaactga tcctgattat gctaaaccac catatgaagc accaattgat gaggaggatg
     3601 aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga ccgtaagaaa
     3661 ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa atctccttat
     3721 caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag tgagcccgct
     3781 tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa
     3841 aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat
     3901 attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc caatcaattt
     3961 ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca agttcaacat
     4021 tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg tggtcaaaaa
     4081 tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta
     4141 gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga tggtagtcaa
     4201 gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca aacgacgtta
     4261 ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac taagaaagaa
     4321 attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg taaacgtgaa
     4381 gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg tacgagacgt
     4441 atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat
     4501 aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg caacaaatga
     4561 atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag gtcaattagg
     4621 acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg tgcttcatga
     4681 caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc
     4741 agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg
     4801 gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac
     4861 aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc
     4921 ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa
     4981 aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga
     5041 agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg
     5101 gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa
     5161 gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa
     5221 tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga
     5281 gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga
     5341 tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg
     5401 attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt
     5461 ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa
     5521 ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt tgatgaaagc
     5581 gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga
     5641 attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca
     5701 tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt
     5761 gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca
     5821 aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa
     5881 caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg
     5941 caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa
     6001 acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagatgacat
     6061 tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg
     6121 gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa
     6181 atacgacaca atggcagcgc aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct
     6241 tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa
     6301 actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt
     6361 cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt
     6421 tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa
     6481 acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt
     6541 tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat
     6601 caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga
     6661 aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg
     6721 gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg
     6781 ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg
     6841 attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc
     6901 ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat
     6961 tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa
     7021 gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga
     7081 tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa aggagcgatc
     7141 gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg caaattatag
     7201 gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg aagataagta
     7261 agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac gtctacgaga
     7321 atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt tgcggtagta
     7381 gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt cgtcagcagt
     7441 atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc gcagactttc
     7501 gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg ccacacttaa
     7561 gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac gaactttggc
     7621 cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct tcaggaacat
     7681 taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag gctattggag
     7741 aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt atgaaaaaat
     7801 tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa taaatgtttt
     7861 ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa aaatattgaa
     7921 gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac gagatacaca
     7981 ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc gaaaatcgac
     8041 aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga tgattccagt
     8101 ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga aaaactagga
     8161 agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga tggggctgag
     8221 tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca aacaggtagt
     8281 atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga tgaatacttt
     8341 gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt catggacgca
     8401 tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt cgaagtagtg
     8461 ataaacgcac aaggctatga caacgaattt tttaagcaga ttaccgacat tagcattcag
     8521 caaggaatgg aagacgaaga cggaaacttt atcgatgaag tagccatttt agtgacttgc
     8581 gagtaagtaa ttgacagcta aagaggagga agcggaatga aactaaaaga cggattttac
     8641 tccagcggcc acggcatcgg cggtttaatg ctagatatgc cgacaaagaa tcctaaaaca
     8701 cgtaagaaac caaaattcaa agtcggtgac atggttcgct gcgaagcaga aggattcatc
     8761 tatccatttc gtggatatgt agaacacgtc tataatcact cagcaatcat tcgcattgaa
     8821 aacacgatgg aatgtgataa gtggttagcg aaaagcaaag agaatttagc agtggtgaga
     8881 ttggtggaca tggaagttat aaaatcttaa ttttgctttt ttacaactat cctacaacaa
     8941 tgagcgttat tccacaactg gaaggagaag aggcaattgt gaacatttta gagaatatag
     9001 atattaagca aacaaggaaa aatgctagac gattactaaa aagatacaga aatttagaac
     9061 gtttggtcgg accagtgaaa atagactttt ccgtgatgac tgttaccaaa aatttgaaat
     9121 tcacaattga cagtcaaaac gaagaaatca ttgaagcgat aagtactcga gattcggtta
     9181 tcgaagcatt aacacggcta agtagaatcc atttccaggt actttattat agttattgtt
     9241 ttcctaataa gatgtcaatg tatcaaatag gagaaaaatt aggatattcc gatagaacta
     9301 tcgagagaat gaaggcagta gctttggttg aatttgctga ggcgtataaa tcaggagaac
     9361 tcatttcacg tacaaaataa aaaaagtcgg attcctccga ctatgaataa tatttccgac
     9421 ataagtatta tatcataatt gggggaatca gaggatggta cttttcgatg taaagaaata
     9481 tgagacacca agcgcgaaag atgttgatat ggaacgcaca aaacataatg tcgctgtgtt
     9541 tctttcagca tatctatcag ctagatgtag agtagggcaa catcgagaac caaaagtgac
     9601 agcatcttat tccttggttc caccttctac agctgatcat atatttgaag cagaaagaat
     9661 gctgatcgat aaagaagaag cacaggaaga atttgagtat ctgcataaat tgtttgttcg
     9721 aggctattca gcgatacagc atccgcataa gcctgatgtg actgaaaggc gcaagaagat
     9781 attctatgat cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac
     9841 tagcgaagaa tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt
     9901 agaactggtt gctttcaagt agccagtttt tacacttttt ataccctttt attaccaatt
     9961 tggtttccat tttatacctt ttttatacca atcacttacc tattcaacgt tgtattatga
    10021 tagtgtcgaa aaattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc
    10081 tcatcgttta attaagcttc gataggcagc aacggaaata ttaagaataa ggatgtgaat
    10141 ttcaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta
    10201 attattcaca cgataactaa aggtaagtga agagaaatga ttccattaat aatttcaatt
    10261 tttgcgcttt gtcttaatgt atatatgatt ggatttaaaa atgggcaaaa taaaaaatag
    10321 tagcagccaa gaataatttt atagtgtcac tgtggcggaa agggtagacg ctaagcatgt
    10381 gtgctaggtc aatgcttcgg caaccatgca aggttcgatt ccttgccagt gactttggca
    10441 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
    10501 cgtgtaggtt gctattacat aactggttag gttagattga gatttgggat tcggtacaaa
    10561 tgaatcgtca aatgtctcaa gcacaggatc ggaaacgtcc ctgcctgtgc attacatatt
    10621 agatcactct ttgagtggtc tttttcttta cataaaggag gctacataat gagaaactac
    10681 tggtatgtat cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt
    10741 gtgcgttctg tacaaatcaa agggaagtac tctattgtcg aaatggggag agaagcagaa
    10801 ccatgtgaga ttgatgcgtg caagctcgtt tattgcggtc atggtttcta tgatgaacca
    10861 aacattcaaa ataatattaa caagaatttg agggattaga atgcaagaaa tggcttaaaa
    10921 atctccaatc aggacaaatc aagttagaca aagtttccga tttaaagata ttgattgaag
    10981 ctgatttaat gctaaaagat attgaaaatt agaaaacaaa actcaaccta aacagaatgt
    11041 gaggtggtgt gtattgaatg gcaagacaac gtgatccaag acgtgatgaa gccaaaagaa
    11101 tttggttaga atccaacgga gaaaagcagt taaaggaaat tgcatctgaa ttaaatgttt
    11161 cagattctca ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa ttgattagta
    11221 acgttaccaa tggcaaaagt aacgttacta atcaaggtgg cgctcctatt ggtaatcaaa
    11281 atgctaaagg taacaaagga aatagccgag cttctccgcc agtgggtaat aaaaacgctt
    11341 tgaaaacagg tgagtatgaa acaatatttt ttgacacgtt aaccgataat gagaaggaca
    11401 tctattctag tttgaatgat gatccttctt ttgctttgtc cgaagaaata cgacttctta
    11461 aaataagaca acttcgaatg atgaaaagaa tccaacaagc tgaagctgga ctaaatgatg
    11521 aagaagtcga acgattgcag cagctaagaa agattaaaac tccgattgaa aaaaatggta
    11581 aaaagctaga aatcaagcgt gaggttatgc aagatgtgca gattagcaga aaaaaacatc
    11641 gcaaaattga tgatattctt tcaattgaag attcattgac tcggattagc aaccagttag
    11701 ctaaagccat caagcaaatg aatgaacttt atatgaatga atacagaact gatttaatta
    11761 aagctcagac tgataagatc caagctgaga caaatgaaat tggcggaaat aattcaggtg
    11821 aagaaataga agaatggaaa caggcagttt taaatgccgc aaacaaacgg gcggtgaaag
    11881 aaaatgaata atgaatttat tccttttgct gatattggtt ctgccattga ttattactat
    11941 gataaaccag tagctttttg ccaagatatt ttgcatttga atcctgatga atggcaagaa
    12001 aatgttttaa atgatttagc tgaattttca aaggtttctg ttcgttctgg tcaaggagtt
    12061 ggaaaaacag cattagaagc aggagcaata ctttggttct taacgtgtcg accctacgct
    12121 aaagtaatag caacagctcc gacaatgaag caactttacg atgtactttg ggcagaggta
    12181 gctaaatggt taaatgatag cttgatcaaa aacttactga agtggacaaa gaccaaaatt
    12241 tatatggttg gtgattcaga gcgttggttt gctacggcta gaacagcgac taaaccagaa
    12301 agtatgcaag gttttcacga ggaccatatg ttgattgtgg tagatgaagc ttctggtgtg
    12361 tctgatccaa ttatggaagc tattcttggt acgctatcag gttttgataa taagctgttg
    12421 atgtgtggaa accccaataa tattgaaggt gtcttttatg attcccataa ttcagaccgt
    12481 gataaataca gagttcataa agtatcaagc tatgatagta aacgtacaaa caaagacaat
    12541 atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt ccgtattttt
    12601 ggagaatttc cgaaaggtgc gttggattca tttatcagtc ttgaaacagt tgaattggct
    12661 acagaaaaac aaattagtga ttctttagtc aataaaacaa aggttgctca tattggtgtc
    12721 gacgtagctc gatatggtga tgattctacg attctctttc ctagaattgc taccagggca
    12781 ttggagtatg agaagtattc aaaacgtagc accatggaaa caacaggata tgtcatcaac
    12841 atggccaaga atctaatgag tcaatatccg agtattgata aagtgatgat taaagtcgat
    12901 gacactggtg tcggaggtgg tgtaaccgac cgcctagaag aacttataga agacaaacat
    12961 tatccttttg aggtgtttgg agtgaataac ggttcaacat cagaagacga tttttacgat
    13021 aatttaggta ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa tatgacagca
    13081 aatcttaacg gagaacagcc tgttattgaa ttgccttctg atagttcgtt aatcaaagaa
    13141 ttaagtactc gcaaattcaa aatgacaagt agaagtcgta tacgtttaga aagtaaagat
    13201 gatatgaaaa agcgaaatat tggtagtccc gatattgctg acgcactggc tttagcgttt
    13261 tatgagccac caagtcacta tcaatttatt caattttagg aggtgagctt ttgattagta
    13321 cagtattatc tttagagaga tataagaagc tacgtgttaa atatgcaaca caaattgaag
    13381 atggaatgtt tgatccgaat ggttttatag aagatatgaa accatttttt gctgatcgcg
    13441 aaagaaaata tctagcttat actagcgaaa agaatgaaat agataataga ccaaagccta
    13501 acactgatat tgtaaaagtt aataataaac ttcatgctgg tatgtattca attgtcgtag
    13561 accaagcagt caatcatttt actggtatac ctatcaaatg ggattacgat gtatcggaac
    13621 aaaaaagaac actcattcaa agattaaaag ataaattctt aaaaaatgat atagaacttc
    13681 cgacagttcc agaagctttt aacaaattaa caagcaatct tgattccatg agattcgcaa
    13741 tgcttgattc tgaaactgca acttttcaag gagcttgtgg agtagctttc cgattgctag
    13801 agcctgtgga agaagatgat ggttggaagt taagagcaag taatattgaa ccttggagag
    13861 cagaaagata tggaaatgcc ggaatctata tcaaagaaaa gtatgactct taccagaaaa
    13921 aattttttca agaaatgaaa gttatgacaa gaaataaaat tctgacatat gcttgctatg
    13981 gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc gataaccctt
    14041 tggggacgat taccttggca gaatttaaaa ataatacgaa tcgttattgt gattttgaag
    14101 tagctgagga aattggagat gcaattgatc gtgctttgtc tgatcaacaa aacgaaatcg
    14161 aacaatttaa gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa ggcactgcta
    14221 aagaaatgat gaatcagcta gggattatca acttaaaagc ccctactgca aaagctgaat
    14281 atgtcacaaa aaatttagca aaagatttca atgaatatca tatggatcta ttgaaaaaac
    14341 agttttacac catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa tcaaacagtt
    14401 ctggagaagc tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat actaaagagc
    14461 aatattttag agaaggattg aaagaatgcg cagaaacaat tgctgctttt cttaagtttc
    14521 atgacaaagt agaaattgag cctgaaaaaa ttatttttac tttctctaga tctttgccaa
    14581 ctgatttaag ttatttagct gaagctttac ctaaacttgc accatacgta tccaaacgaa
    14641 ctattcagag ccaaattcca tttgtaacag atgttgatta tgaaaatgaa atgatggaat
    14701 tggaaagtgg aagtgcttat ccagatagtg aatacaattt tggcggaggt ggcaatagtg
    14761 acgatagaaa cgaagtattg gaccaaacgt cgagaactgg aggatcaagc aaggctcaaa
    14821 caagaaaatc agacacttaa aaaattaact agtgtatttc ctgaagcact gaaagagata
    14881 caagcaaaac tattatcaca agctgactta cacaatatca cttatccaga aatgatggag
    14941 ttttacagta caagtaatca gaaaaaatat cgtgaatatg tggaaaaaaa ttataagtca
    15001 ttaaaaatgt atgatgcaaa atacaaagag tttatcgatg aattttttcc accatttgac
    15061 tatgcaaaag tcaatcgctt attacaaata cgatcagatg tatttaaaat tcttgcagaa
    15121 tatgcgatgg atgcagatgt gaatcaatat ttttctgatc gcttagagga aattcttcaa
    15181 agaacatatt cttctaatgc taatgttttt gttcaacttt taaacgttga tataccaaat
    15241 tatttaccag aaaatgaaat taaacattat ttgaactatc catggtcagg aaagacattt
    15301 tcaagaagac tttggggaaa tatttcatcg cttgaacaga aactctctaa tgctattgta
    15361 aaaagtgttg ctagtggaga aggcgttata cacgcattaa acaccatgag attagactca
    15421 gaaatttgtg acatgtttaa gttagaagaa tcaaagtata acaaagcgat agaaaatctc
    15481 gttcgaacgg agtatgcaaa atttgcacaa gatggtattg aaaaatcata tttagaaaca
    15541 ggtattgagg aatacaacgt attgactgca aaagatgaga gagtttgccg gatttgtgga
    15601 ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg tcataggtga aaatcgagca
    15661 cctttccata gtcgttgtag atgtacggat gttcctaatt taccaaaatt aggaaaggat
    15721 attgatgaag aatatgaccg tttatttggc gatttattag atgagtttgc acatgattct
    15781 tttggaatta atttgaaacg gaggaagtag aatgaaagat ttttttgaag cagtactaac
    15841 aattaatgta aatgctgata ttgcagaagc ctacaaaaca gctattgagt ctgagaacca
    15901 tcctaatggc ttgagagacc attggaatgg caattatgcc tacgtggtta ttggcgatca
    15961 aactgttaat tatcaagata atactccagt tgataagaat accgttaatt taacgattca
    16021 attattatct cattcattac caaatttaaa agaaacagtt gattggtatg aaaagatggg
    16081 atgtattgtt gttagaactg actacaaaga aggaaagtct agtaattagg cttttttatt
    16141 ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg gttgaatggt ggggcgcaac
    16201 tattaaaact caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa ggagaatgag
    16261 catgaaacac aaatcattaa tgccaatgaa tttgcaatac tttgccgaag gtgacgatca
    16321 aaagttttct tttgatgact tcaaatcttt tgtggaatcc aatgaagaag ctcaaaaatt
    16381 tattcagtca caatctcaaa gtgttgccga caaacaattg gaagcttgga aacaaaataa
    16441 cttagataaa attaaacagg ataccatcaa ggaatatgag gaatctaaga aaaacaagtc
    16501 acctgagcaa attcaactgg aaaaactaca agctgaattt gaagcagaaa aagcgttgcg
    16561 tgtgacaagt gataataaag catttgttgc agaacaaatt gctggattag aactagatgg
    16621 agagttaaaa gagtctattt ctcaatttat gctaaataat cttgttagtt cggatacaga
    16681 tttcactaag aatgctgttg aaggttttac aagtgttttg aatgcaatta atgagaaaca
    16741 tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc aacaatcgaa
    16801 taaccaggtt cagcagaaca atgaaacatt aacaaatcca gaagaacaat taggacaaat
    16861 tcttcaacaa tttaactagg agagtgaaaa attatgaaaa aaacatcttt aaataattta
    16921 gagtatttgg atatttcacc agcgattaat gctatgcaag taccaaatac acctttttta
    16981 agctatttac ttggtgctgg aaaaacagag ccagcaaact cgacagaaat taaatggcgt
    17041 gaatatgata tcaacaacga tgattcttct gaaaaacttg agggcggaga atatccagat
    17101 gctgaatcag gtcgaacttg gtttaacaac tatactgaaa tttttagaaa atcaacctct
    17161 gtatctggta cattagatgc tattaatgtg aatggagtcg gaaatgaatt aactaatcaa
    17221 gtagcactac gtggtatgga aatgaaaatt gatttgaacc gaaaattgat tactggtgta
    17281 aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa cttgatcaat
    17341 tcagcaaata aggcagaaac agccactgcg ggtgcagtaa caagaaaaga tatcgatact
    17401 ttatttaaat tgatgtatga aaaaggttat atgggagaaa aactatgctt gatttctcca
    17461 gatatgcagg agttaatgac tgatgagtta gatggaaaat caacaaaaat tgttcagttc
    17521 ggggaaagag taacttttgg attgcaaatt ggaaatatcg tgtctaatta cggtacaggt
    17581 attgctctac tagaaccatc attgccaaaa ggaacaattg ccgcaatcga tactaattat
    17641 gtgaaactac gtccattacg tgaatggaga gcagaagaac ttgcaaaaac aacggattct
    17701 agacgtatcg gtcttgtagg tgaatactct cttgaataca atgcttcaaa ctctggggca
    17761 attttaaatt taaagtctga ataaaaggga gttagtactc ccttttttga taggaggaat
    17821 tacagtggta aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata gagtaggtaa
    17881 aactaaaaat tttgttgggt ttgttcatcc taaaactcgt agatttatca cagcagattc
    17941 aaataacgaa tttatcattt ctatagatga taaagaagca attgcaattt tggaagatgc
    18001 aattgatgtt aatgaaattt aggaagtgat ctgatggatg aatcgctaaa aacggaaatc
    18061 attgagtcta caaaagaaga ttttccagat ttgagtgaag aacgcataac taatttatta
    18121 gaaataattt tgctagaaat tgaatcatac aacacttgta aaaatgatat ttcatgggaa
    18181 aagttaaaaa gcgtgattag tgaagtgttg tataaaataa taaaaaatga atcagaaaaa
    18241 acagtatctt cagttagacg tggcgataca acgattagtt atgcttcaac aacaaatgat
    18301 gttagtgagt tacttctagg ctatggtgat ttgatacgaa gagttattgg ttgtggagga
    18361 ttggagtttt tttaatgaat gaagcagata ttttagaaac tacttatgaa gatagttgta
    18421 ttattgaaag actgacggac attgaagatt ctaatacaaa tattactatt caagattaca
    18481 aaaaagtata tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag
    18541 cagtcataga agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc
    18601 atcctaagat taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct
    18661 taattttttc tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt
    18721 tgataggaag tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt
    18781 gagaatttta aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat
    18841 tcaatgaata aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta
    18901 aggcgaggat atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagaa
    18961 gttataaaca atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt
    19021 ggctttgtca aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat
    19081 attcctcgaa gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa
    19141 agatgaaatc attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat
    19201 atatcttgat tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga
    19261 atctggaact gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc
    19321 attgattgat aataacccta atagaaattt aattaatgaa ttagtctcac gttgtgggac
    19381 tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga
    19441 tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg
    19501 gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc
    19561 tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc
    19621 aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg
    19681 ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc
    19741 aaatggatat actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg
    19801 tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa
    19861 atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga
    19921 tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc
    19981 atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt
    20041 ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga
    20101 actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa
    20161 gacgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaattttt accaggaaca
    20221 gttgtagaag atgctgcagg agttatggaa gaaatgcaaa cagcgactga taagcaatca
    20281 gttaaaaaag ctttaagtcg tgcttattca tttattgcag atactttatt tgagggacaa
    20341 tttactggtg aagattattg caaaggaatt gatgctcgtg agattgcctc attgacagga
    20401 aaattattga agtctgttac tgcaggtttt gatgaaactt atacagagac gaaaaaaaag
    20461 taagtgaggc gctcaagtca ccttcattta agtattcgat tacttaccga gaattagata
    20521 taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt actgacttgg
    20581 atgagttaat gcgtcttttt gcttttagag atgcggtaaa agagcatgaa gatgttgagt
    20641 actatgataa tttcactcaa ttttaggagg tgatactttg aataacgatg acctaattct
    20701 gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt
    20761 gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag
    20821 tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc
    20881 tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa
    20941 taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat
    21001 cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc
    21061 gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat
    21121 atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga
    21181 agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa
    21241 ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat
    21301 tacaatggga gatctgaaag aaggtttatc tcaaggtaag atttctgttg aagaattcca
    21361 aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat
    21421 cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac
    21481 acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg
    21541 tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat
    21601 tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa
    21661 atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg
    21721 agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa
    21781 gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa
    21841 tccatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat
    21901 ggcaaccaat gaagagctta gaaacaaggt tatatctatt tggaatgatg tgaaagattc
    21961 cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa
    22021 agaaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg
    22081 gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt
    22141 agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg
    22201 gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atggaatcaa
    22261 aggttctatt atgtcagttg ctggtccatt gattacaggt ataaagaatg catttttaca
    22321 tgttactttt tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt
    22381 tgagatttta aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg
    22441 tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg aataatatta aagaaagtgc
    22501 cataaatatt tgggaatcta taaagaatat atttgtaagt tattttacaa atatttattt
    22561 tgctgcactc aatatttgga cagggttcaa gcttacattg ataaatattt ggaatgaagt
    22621 ggtaaatcaa gctaaatcga tttggttaaa cataaagtat ttttttatta atctttggat
    22681 tgatataaaa tattttgcaa ttcagaaatg gattgaattg aagttttcta ttattcaaac
    22741 ttggatcgat ttaaaatata atgccattac tacttggaat aatattaaac agtttttcaa
    22801 agatacttgg aaaaatatta aagatacggc atacaatacg tggatttcta taaaaaatac
    22861 catgattaat acatggaata acattaagga ctctttctgg aatattgtta ctggaattgt
    22921 taattctgct gaaaatgcat ggacaaatct aaaaaatggt gtttcaaatg ctattaatcg
    22981 ggtaaaagaa atctttaatt cgttaaggga agtcaattta tttgaaattg gcaagaacat
    23041 cattgatgga cttataaatg gtgtaaaaga aaagtggaat gctttgaaag atactatcaa
    23101 aggtattgca ggtaatatca aaaattggat taaaggagct ttagatattc attctccatc
    23161 aagatggatg cgtgatatgg ttggtaaaaa tattgttcaa ggtattatcg ttggtattga
    23221 taaagagcaa agtaagttag atcaaaccat gacaaattta gtaaaaacac cgtctgttca
    23281 acctgtagta aatggaataa gtcacctacc aattacacaa acaaagcaaa atatatctgc
    23341 tagtgaaaca aaagaaattc atttacattt aaatgtttat ggagatttac ctgattcaat
    23401 ggttcggaag attaccacaa aaattaaaac agaattgaca agacaaatga aacgagatgc
    23461 tggtgcagta ggaggaacat tatatgcaac ttaagagagg acaatttttt attaatcaac
    23521 attattcttc tgaatttaat gtgtatattc aaaacaggcc tgcttctgtt tcagctagtc
    23581 gagtaattga attgagagag cgagaaggaa atgattctat cattattgat aaaggctatt
    23641 acaaaaatgt gactagaaag attgagtgtt actataaagc accttcaatt gatatggtgc
    23701 aggagtggga agatcgaatc actgaatggt tagatatgtc gtcttatagt gattttattc
    23761 tttactatga tgaacaatat atttatcaag ctgtagtaat agaaggacct gaatttaaag
    23821 gaacaagaaa aacaggaaat attgtccctt ttgaatttac agtgagtatt cgaccattta
    23881 aggaaaatta caatggtaga tttactattc gacaaacaga aacttttgaa atttataacc
    23941 cagagaagta ttcttcaaaa ccgcttatta aattgagtgg ttctggagat gcttcttttt
    24001 atattaataa ggataaatat gatttgaaat cgttggacag agaattattt atagattcta
    24061 aattagaaga ggcttatcga aaattagatg gtaatttaga acatcaggat caggtcacct
    24121 tatttttaga ttttccattt ttatatcctg gaaaaaatga aattaaatgg acgaaaaata
    24181 ttcattcatt tgaaataatg cctaggtggt ggagaaaagt atgaaaccaa gaatatatag
    24241 tcctactgaa acagatttct caacgaatgg cttaggaatt ttaaaggatt gtacaagatg
    24301 tggaatatat gaagtagcta atggaaaata tgaattagag ttggattatc ctttaggaac
    24361 tagatttgat gaatattttg aaaatgacta tcaaataaaa gcaaagccaa acgatcaaga
    24421 agagtatcat atctttttta ttgatgataa agatatagat acttttttaa atacagtaac
    24481 tatttatgct cagagtcgta caaatcgact tggaagacgg gtagtcactc ttgtggaagt
    24541 ggactctaaa actggtcaag aagcgatatc aattattgaa actaaaatgg ataaaaaatc
    24601 tgacatacga ctttattctg atattacggc tgtttctagc acaatctttg aagcgagaaa
    24661 tgttttaaat tgtattgctg gtgaacaagg atcattgctt caatattggg gcggagaaat
    24721 taaacgtgaa ccatttaagc tttctttgtt aaagcgaaga ggtcgcaata atgttggaac
    24781 gattcggtat ggaaaagata tgtctggttt gaaggtcaaa ttagattgga caggtataaa
    24841 aacaagaatt attccatatg ctgatcctca aagtgaagta ggtacgacta atcgaattta
    24901 tggttcgcca gtagatagtg cctatattaa taactatcct gatgtctata cagaacatgt
    24961 tcagtttaca gaagaacaag gagtaaaaga cattaagagc ttaaataaaa tagctaaaaa
    25021 ttactttaaa acgatcaatc ctggctgtga taagcctaaa gtttctatta cggttgaatt
    25081 tgataagttg actgatagtg aagaagcgaa agaatttgcg aagattagaa attatggtct
    25141 gtttgataca tttaaaatat atcataaaaa atataatatt tatcttgaat caaaggttag
    25201 tggactacaa tacgattctt tatctgaaaa aactttgaaa ttagaagcag gagacattca
    25261 agttgctttt tatcagcaac aagctgtaac tattcaggat aaattaaaag attatgcaac
    25321 gaataattat atgagtgatt ttaatgatta tgtttcttca atgattacag gtcaaggcag
    25381 tgcaggagga tatgtagttt tatggccaaa agaaaaacct tccaacatct ttattatgga
    25441 taatccagat ttagaaaagg caagagaagt actaagaatg aataaaaatg ggattgcttt
    25501 ttctaagaaa ggatggcggg ggccttttaa ttcagcttgg acattagata gtatatttaa
    25561 tgccaatttt atacaaactg gattaatcaa agcagatatc tttcaaaatt cttttaacaa
    25621 gactggagat gtattgaaat tagttaatgg actactccaa atttggaaca acaaaaagaa
    25681 aataatggag ttaactaaaa aaggaatgga attttggaat tctaagggtt caattggaac
    25741 gattggaaca actgattctg ttggtaatcc ttttcctggg gcttctactc caacccctat
    25801 tgaagataat tctttagtta ttcgtacaaa tggagacggc aaatatattt tgatttctcc
    25861 taaagtagga aaaggattag ttttattagg aaatggtaaa gcaatttatt ttggagactt
    25921 agatgtacaa ggaaaactta cagttaaagg aaaagaaata attcctggac aaggtggtgg
    25981 tccatcggga ggtggagaat ctactggtgg gtatcctagt gaattaaaaa cagatgcaga
    26041 aaagagagct tggagaat
//
LOCUS       DADQRX010000035        25617 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.035, whole genome shotgun sequence.
ACCESSION   DADQRX010000035 DADQRX010000000
VERSION     DADQRX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.035"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="J8V14_001336"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="J8V14_001336"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBA0921450.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="J8V14_001337"
     CDS             complement(860..1678)
                     /locus_tag="J8V14_001337"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBA0921451.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="J8V14_001338"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="J8V14_001338"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA0921452.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="J8V14_001339"
     CDS             complement(2967..3923)
                     /locus_tag="J8V14_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921453.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="J8V14_001340"
     CDS             complement(3923..5056)
                     /locus_tag="J8V14_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0921454.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="J8V14_001341"
     CDS             complement(5049..6611)
                     /locus_tag="J8V14_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921455.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="J8V14_001342"
     CDS             complement(6823..7905)
                     /locus_tag="J8V14_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0921456.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="J8V14_001343"
     CDS             complement(7971..8369)
                     /locus_tag="J8V14_001343"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBA0921457.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="J8V14_001344"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="J8V14_001344"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBA0921458.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="J8V14_001345"
     CDS             complement(9098..10399)
                     /locus_tag="J8V14_001345"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBA0921459.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="J8V14_001346"
     CDS             complement(10540..11556)
                     /locus_tag="J8V14_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0921460.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="J8V14_001347"
     CDS             complement(11864..12469)
                     /locus_tag="J8V14_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0921461.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="J8V14_001348"
     CDS             12665..13588
                     /locus_tag="J8V14_001348"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBA0921462.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="J8V14_001349"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="J8V14_001349"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBA0921463.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="J8V14_001350"
     CDS             complement(15703..17334)
                     /locus_tag="J8V14_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HBA0921464.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="J8V14_001351"
     CDS             complement(17348..19021)
                     /locus_tag="J8V14_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0921465.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="J8V14_001352"
     CDS             complement(19034..19351)
                     /locus_tag="J8V14_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBA0921466.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="J8V14_001353"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="J8V14_001353"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBA0921467.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="J8V14_001354"
     CDS             21013..21798
                     /locus_tag="J8V14_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA0921468.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="J8V14_001355"
     CDS             21801..22493
                     /locus_tag="J8V14_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBA0921469.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="J8V14_001356"
     CDS             complement(22767..23138)
                     /locus_tag="J8V14_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBA0921470.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="J8V14_001357"
     CDS             complement(23538..23768)
                     /locus_tag="J8V14_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921471.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="J8V14_001358"
     CDS             complement(23862..24200)
                     /locus_tag="J8V14_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921472.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="J8V14_001359"
     CDS             complement(25128..25244)
                     /locus_tag="J8V14_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBA0921473.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7475 a         5617 c         4518 g         8007 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacactaaa aaagaggctg ggacagaagc gtttaactcc aagaaataag
    25561 aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt tcagcttatt tccgaag
//
LOCUS       DADQRX010000036        24551 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.036, whole genome shotgun sequence.
ACCESSION   DADQRX010000036 DADQRX010000000
VERSION     DADQRX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.036"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="J8V14_001360"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="J8V14_001360"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBA0921474.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="J8V14_001361"
     CDS             complement(937..1518)
                     /locus_tag="J8V14_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0921475.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="J8V14_001362"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="J8V14_001362"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBA0921476.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="J8V14_001363"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="J8V14_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBA0921477.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="J8V14_001364"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="J8V14_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBA0921478.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="J8V14_001365"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="J8V14_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBA0921479.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="J8V14_001366"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="J8V14_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA0921480.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="J8V14_001367"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="J8V14_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBA0921481.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="J8V14_001368"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="J8V14_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBA0921482.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="J8V14_001369"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="J8V14_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBA0921483.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="J8V14_001370"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="J8V14_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBA0921484.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="J8V14_001371"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="J8V14_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBA0921485.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="J8V14_001372"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="J8V14_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBA0921486.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="J8V14_001373"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="J8V14_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBA0921487.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="J8V14_001374"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="J8V14_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBA0921488.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="J8V14_001375"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="J8V14_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBA0921489.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="J8V14_001376"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="J8V14_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBA0921490.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="J8V14_001377"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="J8V14_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBA0921491.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="J8V14_001378"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="J8V14_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBA0921492.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="J8V14_001379"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="J8V14_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBA0921493.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="J8V14_001380"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="J8V14_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBA0921494.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="J8V14_001381"
     CDS             13972..14157
                     /locus_tag="J8V14_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBA0921495.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="J8V14_001382"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="J8V14_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBA0921496.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="J8V14_001383"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="J8V14_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBA0921497.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="J8V14_001384"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="J8V14_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBA0921498.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="J8V14_001385"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="J8V14_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBA0921499.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="J8V14_001386"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="J8V14_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBA0921500.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="J8V14_001387"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="J8V14_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBA0921501.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="J8V14_001388"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="J8V14_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBA0921502.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="J8V14_001389"
     CDS             18232..18879
                     /locus_tag="J8V14_001389"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBA0921503.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="J8V14_001390"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="J8V14_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBA0921504.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="J8V14_001391"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="J8V14_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBA0921505.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="J8V14_001392"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="J8V14_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBA0921506.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="J8V14_001393"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="J8V14_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBA0921507.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="J8V14_001394"
     CDS             20317..21255
                     /locus_tag="J8V14_001394"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBA0921508.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="J8V14_001395"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="J8V14_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBA0921509.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="J8V14_001396"
     CDS             21935..22774
                     /locus_tag="J8V14_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA0921510.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="J8V14_001397"
     CDS             22750..23619
                     /locus_tag="J8V14_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA0921511.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="J8V14_001398"
     CDS             23609..24406
                     /locus_tag="J8V14_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBA0921512.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         7953 a         4548 c         5279 g         6771 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa a
//
LOCUS       DADQRX010000037        24335 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.037, whole genome shotgun sequence.
ACCESSION   DADQRX010000037 DADQRX010000000
VERSION     DADQRX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.037"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..2477)
                     /locus_tag="J8V14_001399"
     CDS             complement(132..2477)
                     /locus_tag="J8V14_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBA0921513.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="J8V14_001400"
     CDS             2756..3658
                     /locus_tag="J8V14_001400"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBA0921514.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="J8V14_001401"
     CDS             3869..5644
                     /locus_tag="J8V14_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBA0921515.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="J8V14_001402"
     CDS             complement(5710..7296)
                     /locus_tag="J8V14_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBA0921516.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="J8V14_001403"
     CDS             7547..7771
                     /locus_tag="J8V14_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBA0921517.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="J8V14_001404"
     CDS             7913..9664
                     /locus_tag="J8V14_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0921518.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="J8V14_001405"
     CDS             9664..11448
                     /locus_tag="J8V14_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921519.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="J8V14_001406"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="J8V14_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="J8V14_001407"
     CDS             12732..14618
                     /locus_tag="J8V14_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0921520.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="J8V14_001408"
     CDS             complement(14659..15561)
                     /locus_tag="J8V14_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA0921521.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="J8V14_001409"
     CDS             15791..17194
                     /locus_tag="J8V14_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA0921522.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /gene="ascB"
                     /locus_tag="J8V14_001410"
     CDS             17194..18627
                     /gene="ascB"
                     /locus_tag="J8V14_001410"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA0921523.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="J8V14_001411"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="J8V14_001411"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBA0921524.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="J8V14_001412"
     CDS             19911..21638
                     /locus_tag="J8V14_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBA0921525.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21711..22004
                     /locus_tag="J8V14_001413"
     CDS             21711..22004
                     /locus_tag="J8V14_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBA0921526.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="J8V14_001414"
     CDS             complement(22098..22958)
                     /locus_tag="J8V14_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBA0921527.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="J8V14_001415"
     CDS             23050..23478
                     /locus_tag="J8V14_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBA0921528.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="J8V14_001416"
     CDS             23640..23816
                     /locus_tag="J8V14_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBA0921529.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="J8V14_001417"
     CDS             23843..24289
                     /locus_tag="J8V14_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBA0921530.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4352 c         5186 g         7089 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaat
//
LOCUS       DADQRX010000038        23829 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.038, whole genome shotgun sequence.
ACCESSION   DADQRX010000038 DADQRX010000000
VERSION     DADQRX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.038"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..789)
                     /locus_tag="J8V14_001418"
     CDS             complement(55..789)
                     /locus_tag="J8V14_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBA0921531.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="J8V14_001419"
     CDS             1116..1526
                     /locus_tag="J8V14_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA0921532.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="J8V14_001420"
     CDS             1519..2220
                     /locus_tag="J8V14_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBA0921533.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="J8V14_001421"
     CDS             2220..3806
                     /locus_tag="J8V14_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBA0921534.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="J8V14_001422"
     CDS             3825..4046
                     /locus_tag="J8V14_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBA0921535.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="J8V14_001423"
     CDS             4043..4804
                     /locus_tag="J8V14_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBA0921536.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="J8V14_001424"
     CDS             5184..5693
                     /locus_tag="J8V14_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBA0921537.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="J8V14_001425"
     CDS             complement(5768..6307)
                     /locus_tag="J8V14_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBA0921538.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="J8V14_001426"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="J8V14_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBA0921539.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="J8V14_001427"
     CDS             7374..8171
                     /locus_tag="J8V14_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HBA0921540.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="J8V14_001428"
     CDS             8199..8834
                     /locus_tag="J8V14_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBA0921541.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="J8V14_001429"
     CDS             8854..9627
                     /locus_tag="J8V14_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0921542.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="J8V14_001430"
     CDS             10035..10832
                     /locus_tag="J8V14_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBA0921543.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="J8V14_001431"
     CDS             10810..11223
                     /locus_tag="J8V14_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921544.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="J8V14_001432"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="J8V14_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBA0921545.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="J8V14_001433"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="J8V14_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBA0921546.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="J8V14_001434"
     CDS             16000..16560
                     /locus_tag="J8V14_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBA0921547.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="J8V14_001435"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="J8V14_001435"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBA0921548.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="J8V14_001436"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="J8V14_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBA0921549.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="J8V14_001437"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="J8V14_001437"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBA0921550.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="J8V14_001438"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="J8V14_001438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBA0921551.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="J8V14_001439"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="J8V14_001439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBA0921552.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="J8V14_001440"
     CDS             21907..22485
                     /locus_tag="J8V14_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBA0921553.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="J8V14_001441"
     CDS             22537..23019
                     /locus_tag="J8V14_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBA0921554.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="J8V14_001442"
     CDS             23136..23510
                     /locus_tag="J8V14_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921555.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4286 c         5117 g         6644 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DADQRX010000039        23827 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.039, whole genome shotgun sequence.
ACCESSION   DADQRX010000039 DADQRX010000000
VERSION     DADQRX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.039"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /locus_tag="J8V14_001443"
     CDS             complement(<1..302)
                     /locus_tag="J8V14_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBA0921556.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            695..2389
                     /locus_tag="J8V14_001444"
     CDS             695..2389
                     /locus_tag="J8V14_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBA0921557.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            3077..4954
                     /gene="ltrA"
                     /locus_tag="J8V14_001445"
     CDS             3077..4954
                     /gene="ltrA"
                     /locus_tag="J8V14_001445"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBA0921558.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            5085..5462
                     /locus_tag="J8V14_001446"
                     /pseudo
     CDS             5085..5462
                     /locus_tag="J8V14_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5462..6667
                     /locus_tag="J8V14_001447"
     CDS             5462..6667
                     /locus_tag="J8V14_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBA0921559.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6683..7327
                     /locus_tag="J8V14_001448"
     CDS             6683..7327
                     /locus_tag="J8V14_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921560.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            7338..8144
                     /locus_tag="J8V14_001449"
     CDS             7338..8144
                     /locus_tag="J8V14_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921561.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            8166..8396
                     /locus_tag="J8V14_001450"
     CDS             8166..8396
                     /locus_tag="J8V14_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921562.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8942..9217
                     /locus_tag="J8V14_001451"
     CDS             8942..9217
                     /locus_tag="J8V14_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921563.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            9259..9681
                     /locus_tag="J8V14_001452"
     CDS             9259..9681
                     /locus_tag="J8V14_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921564.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9700..11292
                     /locus_tag="J8V14_001453"
     CDS             9700..11292
                     /locus_tag="J8V14_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921565.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            11304..11639
                     /locus_tag="J8V14_001454"
     CDS             11304..11639
                     /locus_tag="J8V14_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921566.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11786..11980
                     /locus_tag="J8V14_001455"
     CDS             11786..11980
                     /locus_tag="J8V14_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921567.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            12012..14384
                     /locus_tag="J8V14_001456"
     CDS             12012..14384
                     /locus_tag="J8V14_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBA0921568.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            14445..15905
                     /locus_tag="J8V14_001457"
     CDS             14445..15905
                     /locus_tag="J8V14_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBA0921569.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            15916..18120
                     /gene="topB"
                     /locus_tag="J8V14_001458"
     CDS             15916..18120
                     /gene="topB"
                     /locus_tag="J8V14_001458"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBA0921570.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            18359..18952
                     /locus_tag="J8V14_001459"
     CDS             18359..18952
                     /locus_tag="J8V14_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0921571.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            18968..19867
                     /locus_tag="J8V14_001460"
     CDS             18968..19867
                     /locus_tag="J8V14_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBA0921572.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            19870..20355
                     /gene="ssb"
                     /locus_tag="J8V14_001461"
     CDS             19870..20355
                     /gene="ssb"
                     /locus_tag="J8V14_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBA0921573.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            20721..20981
                     /locus_tag="J8V14_001462"
     CDS             20721..20981
                     /locus_tag="J8V14_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921574.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            21422..21628
                     /locus_tag="J8V14_001463"
     CDS             21422..21628
                     /locus_tag="J8V14_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBA0921575.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            21628..21879
                     /locus_tag="J8V14_001464"
     CDS             21628..21879
                     /locus_tag="J8V14_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921576.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            21894..22331
                     /locus_tag="J8V14_001465"
     CDS             21894..22331
                     /locus_tag="J8V14_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921577.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            22324..23031
                     /locus_tag="J8V14_001466"
     CDS             22324..23031
                     /locus_tag="J8V14_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921578.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            23412..23591
                     /locus_tag="J8V14_001467"
     CDS             23412..23591
                     /locus_tag="J8V14_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921579.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         8914 a         3851 c         4948 g         6114 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaaac cactctacaa
      481 aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt aggaatggag
      541 aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag gaagagacca
      601 ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa tcttttaacc
      661 aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta aaacagcttt
      721 ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa atcgggaaat
      781 agaaattgta aaatccaaag ggtatgattt agatttttta aaacaagtga tgccacaagg
      841 tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac gatgtgtgac
      901 agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca tgaacaatcc
      961 tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga ttgatcaaat
     1021 caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag cgactgaccg
     1081 aaataaaagc gcggaagaat acctggaact agcggaatat gctagaaaat tgacttctaa
     1141 tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca cgattcaaga
     1201 agtagatgaa cgagcgcagg aactgatcga aaatctcaaa gggatggatt atcgtgcggt
     1261 gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt tcgatgaaca
     1321 agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca ttggcggagg
     1381 tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg gggaaaccat
     1441 gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt tgtctactaa
     1501 taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga
     1561 agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc
     1621 tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg gaatgatcaa
     1681 tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat atgacgatgg
     1741 atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact tagacaaagt
     1801 ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaatttaa
     1861 gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct ttacttcctc
     1921 aaattcgcgt tcaaatactg atacgcaagt cacagggaaa gatccagaag cttatccgac
     1981 gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt cccaagagaa
     2041 aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga cgtatggcat
     2101 gatttttgat ggtcatagta cgattcgaca ccttgaccaa caacagttgg tcttttttga
     2161 cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt acatggctat
     2221 gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt taattaaaga
     2281 aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg agtgccataa
     2341 tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa gtagtctaaa aactattgta
     2401 ataactctta taacgagaag tcaaatgagt gggtaacgcc acaaagggct tccactgata
     2461 ctccgacatg caacaagtag tttatgcaga ccaacgttgt gtgaagctcg gtgaagtcgg
     2521 ctgaaggata ccgtaaggta aaagaaatag tgaataccac gaaagttctc tagaggtaaa
     2581 gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata tggtgagtat gtacaaacgt
     2641 actgacgaac tagcgaatgt actgtccgaa aggcatagct cctagaaatg ggagagacag
     2701 tgagcaaaaa atcactcatt gtgctaactt gtggttgagt aaaagtcgtt gttatgaaag
     2761 accatgctgc gttacaggcg taggagaagt tagggggcaa aagctagcat ctaagttaag
     2821 gcatgtggat agatataagg gaacagggga agccatcaac atggagagtg catactcgat
     2881 gaagtggtag taagaaatat gtgaatgaca aataacttac tttagtgatg gtgaaagtag
     2941 agtcacgact gatgaaggtt ctgtaatgga actggaggaa caggctcgat tcgtctgtaa
     3001 aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac taaaaaggtc acgagccaat
     3061 tggagcgtga taaaccatga caaaagaaac aagtccaaaa gataggaagt tgcggcatgc
     3121 agaatattat ggcatgacgg aaatcttcga taagatatac tccaaaagtt tgaagggaga
     3181 taatttcaag aatctaatgc agattatcat ttctgataac aacattgtcc tagcatatcg
     3241 taatatcaag agaaatcacg gtagtgtgac aagaggagaa gatggagtaa cgattaaaga
     3301 tattgaaaat ctcaaccgta atgaattcgt agaaattgtt aggaagcgat tcgcacatta
     3361 taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat aatggtaaat taagaccgtt
     3421 gggtattcca tcaatatggg atagaatcgc gcagcaatgc atcttacaag tattagaacc
     3481 aatatgtgaa gcaaaattca ataatcacag ctatggtttc cgcccaaata gatcagcaga
     3541 acacgcaata gcagattgta tggcccgaat gaatcaaagt cacatggaat atgtggtaga
     3601 tgtagatatc aaaggtttct tcgacgaagt caatcatact aaactcatga gacaaatctg
     3661 gtctatgggt atacgagaca aacagcttct cttaatcatt aggaaaatgt taagagcacc
     3721 cgtagtactt ccaaatggga agttgatata cccaacaaaa ggaacaccac aaggcggaat
     3781 acttagccca ttacttgcga acatcaatct caacgaattt gactggtgga tagcaaatca
     3841 gtgggaggaa aaagatgcca aagagctaaa acatcatttt aggaaaaatg gagcttggga
     3901 taaaggtaat atctatcaca agctcagaaa gagtacaact ctaaaagaaa tatacctagt
     3961 gagatacgca gatgatttta aaattttctg tagaaattat aatgaagcga aaaaagtttt
     4021 ctatgcaact aaaatgtggt tacaggagcg tctaaagtta ccaatatctg aagaaaagtc
     4081 aaaaatcaca aatctttcaa aagaagaaag tgaattttta ggctttacga tgaaattagt
     4141 gaccaaaggt aacaaaaagg ttgttaatac gcatataggt acaaaagcac aaagcatgat
     4201 taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa agtcctaata gcaaagatac
     4261 ctttatctat attggagtct ataacagtat ggttattggg attcataatt acttcaaaat
     4321 tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga gaactcagac caagttttga
     4381 aatacgacta cgagaacaag gaatctcaaa agaaggacaa tacggaaagt acgaaggata
     4441 caagccttac ctacggtcag gacaaatacg atatataaga gggtttccaa tattaccagt
     4501 agggtatatt caacacagac atccgatgtg taaaaaaaga aatattaata aatacaccga
     4561 agaaggtagg gaagccattc ataagaagca acaaaccgta gaagaatgga agatatcgtg
     4621 gttgtgtagc aatccagtgc ttagtaagag agcaacgatt gaatttgcgg acaatcgaat
     4681 ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca ggtgaggagc ttatcttatc
     4741 tgaaatgcat tgtcatcata ttatccctta tcatgaaagc aaaagtgata gctatgaaaa
     4801 cctagtgatt gttactgagg aggttcatcg ggtaatacac gctacacaaa gtgaaacgat
     4861 tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa aaagaaaaac tcaatgagtt
     4921 gagattaaaa gttggtaacg aagaaatatc ttaataaata tttgtaggac gggaaaacag
     4981 aatgtctttt atagacgagg gaacgccgta tgcggtgaaa gtcgcacgta cggtgtggac
     5041 tgggggaaaa gacggagata atatcaaact cttacctatc agtatcgatt aatccagatt
     5101 ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa gtagaagcaa
     5161 tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg tcttctgata
     5221 acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt atgcgtatgg
     5281 atgaatctat tttacgtcat atagaaggat tagtgggtaa atcaatgacg aactcagaat
     5341 tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc tcaaaagaaa
     5401 ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat ggaggaaaat
     5461 aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg caaagcggcg
     5521 cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga tcactgtgtt
     5581 cgtgacccta tttctaatac ttgcggcggg ctcgattgat aattcggatt cagattcaag
     5641 cagtggtggc gaagctttta caggagaata ttcggaaggc ttaccgattt ataaagaaat
     5701 caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gccgtaggtg cagcagtgaa
     5761 atataaatta ttaccaagtg tgatcctttc gcaatacggt tacgaatcag cgtttggtac
     5821 atcggcgtcc gcaagaaatg atctcaacta ttttgggatt acttggtttg atggttgtct
     5881 gtttcctaaa ggaactgcac gaggaattgg tggcattgaa ggtggctggt acatgaagtt
     5941 tcccaactca aaagctgcat tttcctatta cggctttatg gtagccacac aatccaattt
     6001 taatgcttgt gtaggaaata aaagccctgg cgcaagctta ttgatcttag gacgaggcgg
     6061 ttatgccgct gcaggaatta cggaagatag tccgtattat acaggttgta tgtcaattat
     6121 cacttctaat aaattaaccg aatatgatga attcgccatc aaacattggg gtgaaggtgg
     6181 caataacaac ggcacgatta ctggtgaatg gacgaatcca ttccctggca gcagtttaga
     6241 taaaagttcc ttcagcggtg gtcaattatt tggaacgaat ccaggcggtg aattccgtcc
     6301 gaacggcttc catgatggat tagattttgg ttcagttgat catccaggaa gtgagattca
     6361 tgcggttcat ggcggaaaag tcgtgtacgt tggcaatcct ggtatttcag gtttgggtgc
     6421 gtgtgtgatc gtgattaatt acgatggctt gaatatggtg tatcaagaat ttgcaaatag
     6481 tacaggaaat tctcgagtga aagtaggcga tcaagtgaaa gttggtcaag taattgggat
     6541 acgagataca gcacacttgc atttaggatt tactcgaatg gattggcgtc aagcacaagg
     6601 acatgcattc attgatgatg gtacatggat cgatccgtta ccatttttga atagcagtaa
     6661 aaaatagatc aggagaaaaa atatggaaaa taaaacgtta aaaatcgtct taacagctgc
     6721 aattagcgtt agtttaatcg gcaatattgc gttgttttgg cagctgaaag aggaacaagc
     6781 agcaaatacc tataaaattg aagaagtggc taaaaacgcg tctgagaagc tcaaaaaaaa
     6841 ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg agaaaaacca
     6901 agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag taactgaagc
     6961 acaaagaaag acggcagaag agttcggtca attggccatt gataaatcat taagcagaaa
     7021 tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca aattaagtcc
     7081 tacagacgat gatcaccaac atgatgatta cggaagttac tcctttacat taggtgacgt
     7141 gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa aattttgtcg tatttgttga
     7201 ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca caaaaaataa aaggtggatt
     7261 aactgtcact gaaaaacaag tagatggtca atggaaagtc atagacttta gttatttcac
     7321 tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag acaacgtttc aaaaaacgaa
     7381 aaagttatct tagatggctg ggaatcttta tgattctcgg ctttttgatt cttgctagtc
     7441 cgatttatac gaatcagaag gtagatatac cagatgaggt aagcacgaac gcctttttaa
     7501 acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc gaaccagcaa ttagtttttg
     7561 aatttgcgat cgatgatgaa gatcaaagct tgttaaaaga actggctaat cttaattatc
     7621 gcgtagaagt aaagacagct aaaggggatt atcaagcaat acgagccaaa gtgattaagg
     7681 tgtctgatga ttattttgca gtgaagctaa cgcatgtacc agaagaatat atcgccatgc
     7741 gtttaaccat tattcctgaa aaaattgatc caaaagtgga tatgcaagaa ccacaagatc
     7801 tgatttttta tattcacgaa gataaggtca aagatcacgt gaaagacgaa gattatgaac
     7861 aacatgcgat caattacaaa gtgaaaggat acaagaaaga acaaaaagca gctcaaaaac
     7921 aaatggaatc tttccaagca accattgatt tgaatgaaga attagtgaag aagctaaaag
     7981 atcaattgcc ttatcaagtc gcagatgatc aggaaaatac tgaaaacaag attaatagct
     8041 accagcaaga aattgaaaca tcgaaaacgc aactgaaaga tcaagaagag atcattcaaa
     8101 aacttcaaga aaaaattgat cgtatgacaa aagaaaagat ttagaaggac aggtggagtg
     8161 aaaaaatgaa gcgaacaaac ttggctgaca aagatacttt cgctgagcag aaagtattga
     8221 ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcgagaagtg aatttatcta
     8281 acacaacaat atctgagttg aagaatggtc gaaagaattt agatacctta aaattcagat
     8341 catttgaagc tctatatgta gctgctaaaa aatattttac agctgataac aaataaaaaa
     8401 caggagtatt ttatgtgaaa aaagtatata aaaaatgctt atgaatcagc tatatcacct
     8461 taaccatttg ttaatgagat gggagaaaac attaaccacg attctaacgc aaacatcaag
     8521 cacataaaac atttaacact cctcctttct ggctgaagat ttcaaccaaa caggaagaca
     8581 gcgtatcaat tatagcatac actttttctt aaattcattt ttcatatgac aaaatatttc
     8641 cttctaatcg acaaagttag aaggattttt tattgtctaa atacacaaat aaccttaatt
     8701 tttaaagagc ataaggaaac gtgatatcac tttctcacga aaggtgttag gtaggctgtg
     8761 cctaccacgt ttctaagaaa gataaatgat atcaaaattt tggatttgtg tgtagatttg
     8821 tgggtaggat gaaagcgaat gtgggcagta aaataagcgt ttgtgtgtag caaaatcaaa
     8881 aaatgtgtag ggatatgtgt agctacacat atccctacac ataataaatt tggaggaaaa
     8941 tatggactta ttaattagaa atattgaaga aaagtatatt aataaaattg acaaacgttg
     9001 tgaagaactt acggtcaaga ctggcaaaaa atggagtcga aatcaatacc taaaagtttt
     9061 gatcgaaaat gattttgatc acgctcttct gaattataaa aaagaccaat ttgatcggtt
     9121 gttagaaaaa tttgtggata ttcaaactta taatacgaaa actttagaag aatatattgc
     9181 aacaaataat caattaattg gtttgttaat tgaataataa aaatagtatt gtgaatttat
     9241 aaaaggagga aatcaatttt ggcaaaagca acaagaaaac atgtatatct ttacgatatg
     9301 catatcgaag tacttgatga aataatttct actcatgaag atatatctaa ttatagtgaa
     9361 gcagtcagac atttgtgtat gatgtatgat ccaaaaatcg aagaaaaaat aaatccaatt
     9421 caaattaaat tgaatgcaat gggaaaagaa atttctattt taaccgaaat ggtttcagag
     9481 tttgcggatc ttcatttgaa agatacagga atactaattg gtacaaattc aactgtttat
     9541 caagaagcag aagaacgtgt ggaaggaaaa attaaatcca atcaaaccaa aaaatattct
     9601 tctaagttaa agaaaccagt gaatgaatct gttcatacca gagtagatcc atttgatcca
     9661 acgaagattc taaaaagata aaagtagaag gagaacgtga tgaataaatc gcctgcagtt
     9721 attcttactt gtcaatttac cttgcccaat aaaaaatctt tttcaacata cattgattat
     9781 atgacaagag aaaaagcact ggaagaaatg gaacgacgta cgcccgaaga agaacaggaa
     9841 ctgcaacgaa tcaagaatgc tttagaggat ttttatattc ctgaaggaga aactttttcg
     9901 gaaagtaaga cggataaaga attcagtgaa acgtcgaatg aagctcaatc gttgatggaa
     9961 catggaatca attttgacaa gttagaggaa caagatttta ccaaatatct ttcttatatg
    10021 acccgtcact atgcgttaga acaaaagaaa gaactgacgc aaagggaaca aaaagaatac
    10081 caacggttga accaagcaat gaaaaaatat caaacagagc cacataaaaa agcttcagga
    10141 aatcttcctg gagttttttc tatggcttca gacgaagtga aaggaagtga tctgaaggag
    10201 atcaaacaaa tcttccaaag aggacaacaa aaaggctcaa tcatttatca agatgttatc
    10261 tctcacgaca attcgtatct tgaaaaaatg ggcttgtacg atcccaaaac agatacctta
    10321 gatgaagaag gtttgaaagc agctggaaga aaaatgatgg agacattatt cgagaaagaa
    10381 caattgaatg aaacaggtta ctggatggca acgattcacc gtaacactaa gcatatccat
    10441 attcattttg cagttgttga aaaagaaaat acgcgtaaac cgtatactga gattgaaaat
    10501 ggtgtgagtt atatcgtacc gaaggggaag agaagacaag caacattaga cgcaatgaaa
    10561 accaattatg tacatgagtt agaacgtttc tcttatgaaa aggatcgaat gatcggtatg
    10621 gagaaacgaa atttattgac acgaaaaagt gatttgcgta acaccttaac aaaagtagta
    10681 aaagaaccag cacgttacga tcgacacgcc atgaacttat taaatgaagt atataaaacc
    10741 ctacctgaaa aacgttcgga atggaattat ggagatgaaa atcgaaccaa gctttcacca
    10801 tttacaaggg gaaaacttga tgagttaacc aaatatgttt taacaaaaga acctggatat
    10861 caagaatata tacagatttc aaaagcattg aaagaagaag ccaaaagcct ttatggggaa
    10921 agcaaaagag aaacaaagaa tgcagaaaaa aacgccctct ttgatttaaa aaagcgcaca
    10981 ggaaatgcga ttttaacaga actgaagaga caggatagtc aaataaaacc attaatagaa
    11041 agtgtcaatc agatgcaatc tatttacaat gaacgatcct ttcgaacact caaacagttg
    11101 ccaacaaatg aatacaagta tagttatcaa gaattcgtaa agcgtaggaa acaattccaa
    11161 caaagagttg tcacgcagcg aagcttgcgt caattaaatc gaacaattaa tgcacatgaa
    11221 gaaaaatacc acgcaagtaa agcatatgaa gagcaacagc gaagaatcca aatggaacaa
    11281 gaaagacaat aggaggaaga gaaatgaaac aatatttaga tcaatggaaa gtcatagaag
    11341 gatcactaaa agaagaacga atcgaacaac ttcctgattg tttagaaaaa gagcatttgt
    11401 ttcagataag cgaaatgttg agaaacgaac agtttgatcc aaatcaattt ttagttgttg
    11461 agtacccagc tactggtgtg tattgttgta atcatgtgaa gggtgaaaaa tattttatta
    11521 ttcaagaata cgaaggtaag ttagcgcctt attatactac ttgggaaata aatgaagagg
    11581 gaatcaataa tttcccatgt gaaagcattg aagagtctat ttctctcacg gaatgttaaa
    11641 taaatagaga cgtaaaagag tcctgaacgt gattgtccag gactcttttt caatgaattg
    11701 ttttccgcca attcactgag tggtgcgatt caattttatt atatcaaaag aaagtgagat
    11761 tccaaagtga agggaggaag aagaaatgac tcagacaacg attggtgttg tggttgaaaa
    11821 aagcagggat aatctcgttt ttgtgacaga aattcaaaca ggtagagctt ttgtagtcac
    11881 agacaaagct gcaaaagcgt atcaaaatgg caatatttta acgttaaatc tgactacgaa
    11941 gatgttcgtt gatgcagtag aaaattatcc atttgtttag acaaacgata ttttacaagg
    12001 aggaaacgga catggcagaa gaaaaagaac cactagataa tagtcggttg ggaaagtcca
    12061 aaagaaaatt agtacggttg caaaacgaat taaataatca atttcaaaag ctatttgatc
    12121 accaaaaatt gacaaatggg caaccaatga atgacaaacg aaatggtcgt tcatggttta
    12181 accagcaaga aaaaatagaa aataaaatac aaagtttgag agaagagatc aagcaacaag
    12241 aaaaacaggt agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt
    12301 acggcggact ggatatgacg atcgataata ttccaagaat caaagaagaa atcgaacgct
    12361 ttgaaaaagg tgaatcgact ttttcagccg ccacgatccg aaaatatcaa cggaagttgg
    12421 aaacattaga acagctgaaa gaaaaatcag aaaaaggaaa agaaaatatc ctccctgaag
    12481 tacaagcgat cattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt
    12541 ttttgaaagg ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat
    12601 caaaaaaata ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag
    12661 aggctgcacc aactgattct ctgaaggaga aagaaaaaga tcatttctat cgagtagaat
    12721 tcaacgaaaa tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag
    12781 aattgatcga tcaacttaaa acgttggata accaatacgc tgcagaagca ggttattata
    12841 aattctattt tgaagaagtg aagaacggag aagtagtaga tcagatccga atggatctag
    12901 gggatggcgt caacagtaat caccccatct atttagagtt agcaaaattg tgcgaacaag
    12961 tacctgagaa ggaacaaaaa caatcggtca aaaatgaacc tgtttcgaaa gaacaaatgc
    13021 agtcgaaaga aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatca
    13081 aacaagaagt taaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt
    13141 cttctgaaga cgtcaaagaa cttttgaact ttatgtctcg ttttccagaa cgatccttta
    13201 gaaatcaatt attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgccg
    13261 cgttgaaaaa agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca
    13321 gaaaaacggt caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta
    13381 caccagaaga taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact
    13441 acacgattga aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga
    13501 aaatctttcc gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc
    13561 aagctgggat tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa
    13621 gtgaagtcta tcaaagagaa ttaggacaag cacgtggggc gtatgttcgt aacgaattta
    13681 ctggcgaaga agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa
    13741 gtattcatga attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta
    13801 aagaattgca ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt
    13861 cggagaaagc tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata
    13921 aggaagcggc ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca
    13981 ttcaaaccat ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag
    14041 cagaagtgaa gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc
    14101 ccgttggttc actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc
    14161 gaacgttaca tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt
    14221 taaaaggaaa aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc
    14281 taggaaatcc attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg
    14341 aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac
    14401 tatctgaaaa acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa
    14461 tgagtcttat tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa
    14521 tggctgtagt caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg
    14581 atcgaagaaa acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat
    14641 tagctaaact ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag
    14701 ccactgattt tattctaaag aatgaagaca aaacagaacg agtggaaaat cttcattttg
    14761 aaacagaaaa gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga
    14821 attatctgaa ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag
    14881 ggttgattgc cgaactaaag ccaaaatatg aacggcaaaa tttcttagtt cgagacgatc
    14941 gtttagatca tgcggtcgcc tttttatgga aagatccaca gacaaaagag acagtagggg
    15001 cgagttatca aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc
    15061 atatagataa aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc
    15121 agttaaaatt ttttgagagt agtattgatt tacttagcta tgctgcactg aatcgtgatc
    15181 agttaaacga tacgtggtta gtctctatgg aagggttaaa acaccatgtg atcagtcatt
    15241 attttggtga ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga
    15301 tttgtgtgga taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg
    15361 cagtcgatcc atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc
    15421 aagtgccaaa agaatacaaa atcatttatg aagaagtcgc aaaagaagaa aaggtcacac
    15481 cagaagctat tatggccata cacaagacag aaaacaatct gcaattaacg aaccaactgg
    15541 tttccgctca caaagtaaat gcgttttttg gccagcagct ttctgttaac gattcaatag
    15601 aagccattaa tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gtttgtgaac
    15661 gcgtggatgg tacgtacgat tttgatcgtt tttatcaaga aaaaggggat attaatgcgc
    15721 agattctttt ctcttataaa gcagaacaat actacaaagg ctataaaaat catgaacatg
    15781 aatttatacc tgaagtaaaa aaggactgga acgatcaatt gaagcatgag atccagcaac
    15841 aagaaatcag aaagcaaaaa cgtgccatgt tgtttcaaca aggcagacaa caagaaaggg
    15901 agtgaaatcg tcatgatgaa aaccgttatt ttagcagaaa agccgtcaca agcgaaagct
    15961 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
    16021 ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
    16081 cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc tattttcccc
    16141 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
    16201 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
    16261 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
    16321 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
    16381 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
    16441 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
    16501 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
    16561 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
    16621 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
    16681 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
    16741 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
    16801 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
    16861 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
    16921 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
    16981 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
    17041 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
    17101 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
    17161 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
    17221 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
    17281 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
    17341 ctacaagttc ttgaaaaaga aaccaagcca ccacagcctt ataccgaagg tacgttgatc
    17401 acggcgatga aaacagctgg gaaaaccgtt gatagtgaag aagcgcaatc catattgaaa
    17461 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
    17521 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
    17581 tgccaagccg tggaaaaaga gccgctgctt acgagtgccg aaatgaccgc caaatgggaa
    17641 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
    17701 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
    17761 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
    17821 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcatgt
    17881 acgtctgaag cctgtgattt caaactatgg acaacgattg ccaaaaagaa acttacagcg
    17941 acacagctga aagagatcat tcaaaatagc cgaacaagcc aaccagtgaa agggttaaaa
    18001 ggtcagaagg gttcgtttga agcaacgatt gttttaaaag aagacttcac tacaggcttt
    18061 gaattttctg aaaagaaaaa aacgaacttc aagaaaagaa cacgacgcac tacaaaataa
    18121 tggagggtta atcttatgcg taatacaaca cacaaacaac aagaaatcga aaatcaaaaa
    18181 gccaaaattc gttcagaaca ggaaaaaatg cgtgccagca aacgaagact gcagcaattg
    18241 aagaaccagc agcggattca aaaagaaatc gaattagatc gataaattcc tttcttgtta
    18301 atataaaact aacatgttat agttgtgtta gttttatatt aactttaggg gaagagtaat
    18361 gaaagacaaa atccaatgta tttttgataa acataatggc aacctatcct tgaaggatgc
    18421 tagaagtgaa ggattatcgc ctatgacgtt agatcgatta gccgaaaagg ataaaataga
    18481 aaaggtagca ccaggtttct ataccttaaa ggatgcgttc gtggatgaat tgtatctcgc
    18541 tcagagtatc tattccagag ggattttttc tcatgagact gcgctcgatc tttttcaagt
    18601 aggaacatat atccctaaaa agatcaatat gatgtttcct aaaggataca atgtatcaaa
    18661 ggagaaatta gatcagtatg ccattcaacc gcattacact aataaagaaa attttgacct
    18721 gggagtcaca gaaacagaaa gcttttacgg caataaaatt ttagtctatg atttagagcg
    18781 aacgttatgt gacatgtgga atccaagata caaagcaagc gtagaggtaa aacaagaagc
    18841 attaaaagaa tatatgacaa gtaccagtag aaatacaaat aagctaagaa gatacatgga
    18901 tattttaaaa tcaccaaaag aaatgacttt gtatatgttg ccactttatt gagaggagta
    18961 aagatgaatg acaccgacac aatttaaaga gaaaacaaga aatttcgcag cagagaaaca
    19021 tttaaaccca caaattgtcc aaagatatta tatgatggaa aaattattag aatgtattgt
    19081 tgaatcgcct tatcatgatg attttgtatt aaaaggggga tttctgattg gatcaaaata
    19141 cgggttagaa aaccgttcaa ccgttgatat tgatacgact tatcgaaatt cgaaactaac
    19201 aaaagaaaag ttagaaactg ttttttctga attaacagct aatcctacaa aagatggaat
    19261 tcgttttgaa ttaaagggat taacagagac gagggaagcc gattattatc caggttttca
    19321 agcaaagttt attgcttatc ttgaaaatgc acgtgtacct tttaaattag atattacttc
    19381 aggtgattct attattccag cagcattgat gtatgaacac aaattaatgt ttgaagataa
    19441 aacgattcat attccagctt atcctacgga acaaattcta gctgaaaaat tacatgccac
    19501 gtttagtttt ggaaaagata attcgcgcat gaaagatttt tatgacctgt atatgattcc
    19561 gaaattagaa cgtatcgatg agaaaaattt gtataagagt gttcaaggaa cattcaagga
    19621 aagaaataat caatcggtgt ttaagcattt gtatgatcag gaaatgcctc aattggtgaa
    19681 agatgagggc ttgagagaaa agtggaaaag ataccaacaa gaaaactatt ttgccaaaga
    19741 catttctttc gaatctacac taacttccgt tgattccctg atgcaatcaa ttgtagagca
    19801 agagaaggca gaacgaaaac aaaattttaa gagatcacaa caaatgaatc aagaaatgga
    19861 gagataaaga tgatcaataa tgtaacttta gtagggcgat taactaaaga tccagattta
    19921 cgttataccg caagtggcac agcagtggcg actttttccc tagccgttaa tcgtaatttc
    19981 acgaatcaaa acggcaatcg agaagctgat tttatcaatt gcgtgatttg gcgcaaacca
    20041 gcagaaacga tggcgacatt agctaaaaaa ggcagcttga tcggcgttgt cggacgaatt
    20101 caaacacgga cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc
    20161 gataacttcc aactattaga aagtaaagca gcgacagaaa gccgtatgca cgctgatcaa
    20221 tcgtccagca gtccaagccc taccaccttt gaacaacggg atacagcgac gcctaacaac
    20281 aatggattga acgcttcaca gaatccattt ggtggacaat caatcgatat tagcgatgat
    20341 gatctaccgt tctaagtagc attttctatt ggttgcattt gctacggttt atgttataat
    20401 ttagacaaca aaaagaattt tcaatctata accaaaaacc actagctggc gggctagtgg
    20461 ctttctttta cgtttctatt ataagaatta acgggtaggc ttactgtctt tcttggcaaa
    20521 tttacaatca agccatttgc agatatagtg cgcggctact tctgccatga ttgcaaaaac
    20581 aaaatcaaaa agaattttca aacctataac ctcctcccct tgccagatta ggccggggta
    20641 gcagacagca agactattat agcataattg aaatatccaa atgattggat ttttgctata
    20701 attttagcga ggtgattgtg atgaacaaat atgttggttt gcttgataaa atccgagtaa
    20761 taaaaacgca accgttattg gttcgcttta ctttacaaac gattcatgaa tcgattaatt
    20821 gtgtagttgc agatattgaa attatcgata aattgctaat tatggatgat ggaaaataca
    20881 atattgccgt cacaggtcac ttcaataaac gaaatcagct agttattgct agcatgtatg
    20941 ttagaaatcc cgatcatttt acgcgatcga tgggcatata aataaaagaa gagttgtagt
    21001 cctaaatgag gattgcagct ctttttgttt tacatagaaa caggaaaaga cctttttagg
    21061 aagacacaag tgccttctca attgaaaaac ctttctctca ctccaaccgc caccgccgtt
    21121 cgagaagaaa ggaagtgaac gctgcacgtt cactaagata tggtgtcttg caaagcaaga
    21181 caaaatagac aaaacttcct ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg
    21241 atttgccaga ccggcaacaa gttgcctttt atcaccaaaa agcgacaagg acaagggtaa
    21301 atggcacgct gcgcgccctt gtccttgtcg cttttcgttg atttattctg gccaacaaat
    21361 caataaagaa agaactaacg aaaggaagta aaacctttcc taagaggagg cagaatcaaa
    21421 catgcgaaag aaatttgatt attggggtgt tcccttcagc gaattattcc caaactatca
    21481 tgcaccacat actgttgaat gcgattgtgg agaaagagcg aaatgtatca aaagctatcg
    21541 tttgtatcaa tgtccaacat gcgggaaaaa atataccttg tcttatggcg attacatttt
    21601 gattgatgag aaaggaaaga aacgctgatg aaaacacaac acaaattatt gactgtaaaa
    21661 gaagtcgttc aaattttggt ggacaaagaa gtcatttctg aaaaagtagc agaagcttta
    21721 cttattccgc ggtactggac gatagaaacc cctttagtag aggatgcacc gactatgcta
    21781 tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa catggaaact
    21841 tatgaaatcg aatattggtt aaccaaatta gagaactaaa aaggagaaaa aatatggctc
    21901 aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa aaaatggagg
    21961 atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag gaaaaattgt
    22021 cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt gcgacgtact
    22081 atcgaacact ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa gccttactat
    22141 gcttggaagt aggtgtcaaa ccaagcacta tcaaaccttc cgaatatcaa gccttagaac
    22201 tgacttatgc aaaattcgca cagattaaga aagctaaagc aatggctagc gactggctag
    22261 atacttttga aagtatcgaa aaaaatggtg cattttatac gattgaatcg gaggagaaag
    22321 ataatgaata aggtggttta tcagctaaca acagaagatg tttcgcaatt tttaacaagc
    22381 aaagaacact tgaatgaagt aatcagacgg attcaaaaag actttagttg tatggaagaa
    22441 atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga aacaagtcaa
    22501 acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc tgaaacggta
    22561 aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa tgtgtaccta
    22621 gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa acactattac
    22681 tcagaatcag aatattattc aattttcgag agcatggaat taatggaaga agaaatggaa
    22741 gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac taggtttttg
    22801 acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc tgtacccgaa
    22861 gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg gtacacgact
    22921 ttaactgatt ttttagacac ctacacttac gacgatacag aagaaatcca aaatgaactt
    22981 gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtaaata accatttata
    23041 ccgaaaagtg aggatgtgtg aaataagaac aaaaaacctt gaaattaagg gaatggttct
    23101 tatttcgcac caatcgctcc aaccgccccc gccgcttagg tggtgcgaaa taagcgggca
    23161 aagcccgata acctaagaga aagaaggaga tccatatgaa taaaatcatt cctctattgt
    23221 tcgtactgtt tgttggcgag atacttatgc gtcatgctgc agctgtacat ttaaacttcg
    23281 tagtattctt aggaatactg gtattttgtt tgatcttttg gcgatttgta ttagcccttg
    23341 tgagtctact attcaagatg attgcagcca tatttttcat agggttattt cgatgaaagg
    23401 agagagtact aatgccaatt gcaataggaa ataaacggtt acccgtgaca ttagatgaaa
    23461 agagacaaaa agaattgcag caactaaagc agaagtacgg aaaaagtgaa tctaagatta
    23521 tgtgtattgc gttagatcta ttgatcgcgc aagaaaaagc aggatttgat ataccagcac
    23581 taaaaaagtg acgtcacttt tcatttgaag aaacaaaagt gatagcactt aattgttcct
    23641 actatttaga atattttcta tgtccttttt attccatttt gtatcaataa ctatgaaatc
    23701 tttaaagtat ccagaagaga ttatttttag atcaattttt ttcttacctg attctaaaga
    23761 ttttgttttc ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat
    23821 agtttat
//
LOCUS       DADQRX010000040        23642 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.040, whole genome shotgun sequence.
ACCESSION   DADQRX010000040 DADQRX010000000
VERSION     DADQRX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.040"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="J8V14_001468"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="J8V14_001468"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBA0921580.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="J8V14_001469"
     CDS             complement(1171..1866)
                     /locus_tag="J8V14_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HBA0921581.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="J8V14_001470"
     CDS             complement(1879..2595)
                     /locus_tag="J8V14_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBA0921582.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="J8V14_001471"
     CDS             2925..3533
                     /locus_tag="J8V14_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBA0921583.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="J8V14_001472"
     CDS             3858..4181
                     /locus_tag="J8V14_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBA0921584.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="J8V14_001473"
     CDS             4363..5733
                     /locus_tag="J8V14_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBA0921585.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="J8V14_001474"
     CDS             complement(5863..7164)
                     /locus_tag="J8V14_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA0921586.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="J8V14_001475"
     CDS             complement(7518..9509)
                     /locus_tag="J8V14_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HBA0921587.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="J8V14_001476"
     CDS             complement(9653..9919)
                     /locus_tag="J8V14_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA0921588.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="J8V14_001477"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="J8V14_001477"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBA0921589.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="J8V14_001478"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="J8V14_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBA0921590.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /locus_tag="J8V14_001479"
     CDS             13263..13826
                     /locus_tag="J8V14_001479"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBA0921591.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="J8V14_001480"
     CDS             complement(14079..14600)
                     /locus_tag="J8V14_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBA0921592.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="J8V14_001481"
     CDS             complement(14603..15445)
                     /locus_tag="J8V14_001481"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBA0921593.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="J8V14_001482"
     CDS             complement(15438..16163)
                     /locus_tag="J8V14_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBA0921594.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="J8V14_001483"
     CDS             16339..16635
                     /locus_tag="J8V14_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921595.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17203..18795
                     /locus_tag="J8V14_001484"
     CDS             17203..18795
                     /locus_tag="J8V14_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0921596.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="J8V14_001485"
     CDS             18808..19548
                     /locus_tag="J8V14_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA0921597.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="J8V14_001486"
     CDS             19716..21065
                     /locus_tag="J8V14_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0921598.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="J8V14_001487"
     CDS             complement(21193..21783)
                     /locus_tag="J8V14_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBA0921599.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="J8V14_001488"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="J8V14_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBA0921600.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DADQRX010000041        22942 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.041, whole genome shotgun sequence.
ACCESSION   DADQRX010000041 DADQRX010000000
VERSION     DADQRX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.041"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..288
                     /locus_tag="J8V14_001489"
     CDS             <1..288
                     /locus_tag="J8V14_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921601.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="J8V14_001490"
     CDS             347..787
                     /locus_tag="J8V14_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921602.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="J8V14_001491"
     CDS             799..1467
                     /locus_tag="J8V14_001491"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBA0921603.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="J8V14_001492"
     CDS             1531..2154
                     /locus_tag="J8V14_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBA0921604.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="J8V14_001493"
     CDS             2210..2596
                     /locus_tag="J8V14_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBA0921605.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="J8V14_001494"
     CDS             2809..4200
                     /locus_tag="J8V14_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBA0921606.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4547..5194
                     /locus_tag="J8V14_001495"
     CDS             4547..5194
                     /locus_tag="J8V14_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBA0921607.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            5587..8232
                     /locus_tag="J8V14_001496"
     CDS             5587..8232
                     /locus_tag="J8V14_001496"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBA0921608.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            8542..9855
                     /locus_tag="J8V14_001497"
     CDS             8542..9855
                     /locus_tag="J8V14_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBA0921609.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            9845..10498
                     /locus_tag="J8V14_001498"
     CDS             9845..10498
                     /locus_tag="J8V14_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBA0921610.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            10553..11245
                     /locus_tag="J8V14_001499"
     CDS             10553..11245
                     /locus_tag="J8V14_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBA0921611.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            11395..11595
                     /locus_tag="J8V14_001500"
     CDS             11395..11595
                     /locus_tag="J8V14_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA0921612.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11849..13528)
                     /locus_tag="J8V14_001501"
     CDS             complement(11849..13528)
                     /locus_tag="J8V14_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBA0921613.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(13530..13742)
                     /locus_tag="J8V14_001502"
     CDS             complement(13530..13742)
                     /locus_tag="J8V14_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBA0921614.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            14135..15865
                     /gene="efrA"
                     /locus_tag="J8V14_001503"
     CDS             14135..15865
                     /gene="efrA"
                     /locus_tag="J8V14_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBA0921615.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            15862..17622
                     /gene="efrB"
                     /locus_tag="J8V14_001504"
     CDS             15862..17622
                     /gene="efrB"
                     /locus_tag="J8V14_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBA0921616.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            17717..17914
                     /locus_tag="J8V14_001505"
     CDS             17717..17914
                     /locus_tag="J8V14_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921617.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            18067..18552
                     /locus_tag="J8V14_001506"
     CDS             18067..18552
                     /locus_tag="J8V14_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBA0921618.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(18656..19249)
                     /gene="clpP"
                     /locus_tag="J8V14_001507"
     CDS             complement(18656..19249)
                     /gene="clpP"
                     /locus_tag="J8V14_001507"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBA0921619.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            19429..19956
                     /locus_tag="J8V14_001508"
     CDS             19429..19956
                     /locus_tag="J8V14_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HBA0921620.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            20047..20784
                     /locus_tag="J8V14_001509"
     CDS             20047..20784
                     /locus_tag="J8V14_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA0921621.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            20788..21705
                     /gene="miaA"
                     /locus_tag="J8V14_001510"
     CDS             20788..21705
                     /gene="miaA"
                     /locus_tag="J8V14_001510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBA0921622.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            21707..22936
                     /gene="hflX"
                     /locus_tag="J8V14_001511"
     CDS             21707..22936
                     /gene="hflX"
                     /locus_tag="J8V14_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBA0921623.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         7460 a         3945 c         4966 g         6571 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgttttat ttgtctcaca cccttttcct tcctacttgt aaccttgctc ccgtgttaga
     4501 aacatgttac actaaatatg tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac
     4561 agatacactg ccaaaagcaa ctgcgaaaag attaccttta tatttacgct acttgaagat
     4621 gctggatgac tcaggtataa gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat
     4681 tccttctgca acgatccgca gagacttctc gcaattaggc gagctaggca gaagcggata
     4741 tggctatgat gtcccttttc ttatcgatgt attcaataat atccttaata ctaaagaaga
     4801 aaaacgaatc gcattagtag gatatggaaa tcttggcaaa gcattgaaac acaacaattt
     4861 tagaagaaat gaaaatctaa atatcgtgtg cgtctttgat aatgatccag cactgatcaa
     4921 tcgagtaatt gatggagaaa tgatctatcc gattgatcgt ttcgcggaga tagccaaagc
     4981 aaaaaatgtg acagtagcaa tctctacggt accaagtaaa tattcacaat ctgctattga
     5041 tgaaattgtc aaaggaaatg taaccgctat acttaatttt gcgcctgaca gagtaactgt
     5101 cccagcttat gtcaatgttc aatacattga tttgacaacc gagttgcaga cgcttattta
     5161 ctttgatgag aattactcag aagttttttc ttaggaaaat ttgagtaggc ttattgatct
     5221 tgcgagaact cgtcgtaatt ctttcaaaaa ttttttagta ctaaatgaag tggttttttc
     5281 acgaccactt gttgactaaa aaaaaaaacc attgtagaat gaagcataac gctgaacaag
     5341 aggagtaact ggcacattca ttcttagaga gagaactatc tgctgaaagg ttctcaatga
     5401 caaccagtga aggtagcttg ggagtttctg tcttgaatgg agtagagata gacgagtcgt
     5461 gatcgttaac tcacttgaga ggcaataatg attgattatt gcaatttagg tggtaccgcg
     5521 taaatcatac gtcctagaat acgaaagtat tttaggacgt tttttagttt aaaggaggaa
     5581 aacagcatga cggaagaaaa taacctatca acaaaatata atccgcaaga agttgaagct
     5641 ggacgttatc agcattggtt agaacaagat ttgtttaaac cgagcggaga taaaaaagca
     5701 aaaccttatt caatcgtaat cccgccccca aatgttactg gaaaacttca tttagggcat
     5761 gcttgggata cgaccttgca agatatgctg attcgtcaaa agagaatgca aggatatgat
     5821 actttatggc tgcctggtat ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg
     5881 aagcttagag agcaagggat ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa
     5941 gtgtgggaat ggaaagaaga gtatgcgagc cacatccgcg aacagtgggc taaaatgggc
     6001 ttatctttag attacagtag ggaacgtttt acattagatg atggattatc tgaagcagta
     6061 cgtaaagttt ttgttacctt atacgaaaaa ggtttgattt atcgtggtga atatatcatc
     6121 aactgggatc ctcaagcacg aacagctcta tctgatatcg aagttattca taaagatatc
     6181 gaaggcgcct tttatcatat gagctatgaa ctagcagatg gatcaggcgt agtagaaatt
     6241 gcgacaaccc gtccagaaac gatgttggga gatacggcga ttgctgttca tccagaagac
     6301 gagcgttatc aagcgctcat cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc
     6361 ccaatcattg cagatgaata tgtagatatg gaattcggga caggtgtagt aaaaatcact
     6421 ccagcacatg atccaaacga ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat
     6481 gttatggatg atgatggtac gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt
     6541 tttgctgctc gtaaagcaat cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc
     6601 gatacgatga tccacagtgt gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg
     6661 ctgtctactc aatggtttgt aaaaatggcg ccgttagcga aaaaagccat tgataaccaa
     6721 gaaacagatc aagcagtgga attcttccct ccaagattca atcaaacgtt tttacgttgg
     6781 atggaaaatg tccatgattg ggtaatctca cgacaattat ggtggggaca tcaaattcct
     6841 gcatggtatc acaaagaaac aggtgaaatg tatgtaggga tggaagcacc agcggatagt
     6901 gaaaactggg tacaagatcc tgatgtttta gacacttggt ttagttcagc tttgtggcct
     6961 ttctcaacaa tgggctggcc agatacggaa agcgaagatt ataagcgata cttccctact
     7021 agcactttag taaccggtta tgacatcatt ttcttctggg taagcagaat gattttccaa
     7081 agcttggaat ttactggcga acgtccattt gagaatgtct tgattcatgg attgattcgt
     7141 gacgaacaag ggcggaagat gagtaaatca ttaggtaatg gaatcgatcc gatggaagta
     7201 atcgaaaagt atggagcaga tgctctccgc tggttcttat cgaatggttc tgcaccagga
     7261 caagatgtcc gcttcagcta tgaaaaaatg gatgcttctt ggaactttat caacaaaatc
     7321 tggaatgctt cacgtttcgt tatcatgaat atcgaaggaa tgaccgtaga agagatcgat
     7381 ttatctggag aaaaatcagt ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc
     7441 gaacgagtaa ctgagctatt tgatcgtttt gaatttggtg aagcaggtcg acaactgtat
     7501 aactttatct gggatgattt ttgtgactgg tacatcgaaa tgagtaaaga agtactttat
     7561 ggagatgatg ctgtttcaaa acaaaccacc cgtagcatct tagtccatac acttgatcag
     7621 atcctacgtt tattgcatcc aatcatgcca ttcgttacag aagaaatatg ggaacatatt
     7681 cctcatcagg gaaattctct agtagtggca gaatatcctg ttgtacgtcc agagtttgat
     7741 gatgaaacag cttctaaagg gatggaagta ttgaaagaat tgattcgagc cgttcgaaat
     7801 attcgttcag aagtgaatac accattgtct aaaccaatta cgttgctaat taaaacaaac
     7861 gatgctaaaa tcgatcaatt tttagttgaa aatacaagct atatcgaacg cttctgtaat
     7921 cccgaagagt tgacgatatc aagtgagatc actgcgccag atctagcgat gtcagctgtt
     7981 ctgacaggtg cagaaatctt cttgccgtta gcaggattga tcaatattga agaagaaatc
     8041 aaacgattag aaaaagaact ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa
     8101 ctttctaatg aacgttttgt ttctaacgcg ccggaagaag tagtagaggc agagcgtgca
     8161 aaagaaaaag attatttaga gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt
     8221 actattcaat aagaatagaa aaagagagtg tgaaatcagc tagctattac taaaagctag
     8281 ctgattgaac atcgttacag gaaagagttt gagcctcact cttagcttcg attcataagg
     8341 agatgtgaca taaattttgt cgcatctctt ttttagtcta ctcttattat gtaggagaag
     8401 gagggaggtg gatcatgtat caagagtcaa taaagtagtt gataaaatcg ttcctctctc
     8461 tgtttctttt ctctttgtca tacttttatt tttcttatgt gttctatttg ttataataaa
     8521 agagaaatgg gggaattgtt gatgacgatt gaagaagcaa tagaatggat ccatagtcga
     8581 ttaccatttg gttcacgtcc tggccttgac cgagtcgaag ctttattaga agtggtaggg
     8641 catccagaga gaaaagtaaa aacgatccat atcgctggaa caaacggaaa aggttctact
     8701 gtagcttatt tgagaagcct attagaagag gctgggctga cagtaggtac atttacttca
     8761 ccttatatcg aaacgttcaa tgaacggatc gcaatcaatg gcaaaagcat tccagacaat
     8821 gatttgatcc agcttgtaaa aaaatatcag ccactgatca aacaacttga tcaatttgaa
     8881 gcagtagctg gtattactga attcgagaca ttaacagcaa tggcatttga ttattttttg
     8941 atgaagaaag tggacgtagc gattatcgaa gttggcttag gcggcttatt ggattctacc
     9001 aatgtagctg taccaatgct gacgggaata acaacgattg gacttgatca taccgaaata
     9061 cttggagata caattgaaga aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt
     9121 ccagtagtaa caggaaatat tgccccagaa gcttttactg tcatccaaca aacagctaaa
     9181 gcaaaaaatg caccgcttta tgtatacggg gaaaattata aaataaacta tctgcatcct
     9241 gatgagatgt ggggagaagt atttgaattt tttaatgaaa taggaaagat ccctcaatta
     9301 accactccgc ttttagggca acatcaagct gaaaatgcag gagtagccat cgaattgtat
     9361 tttgtttact gtcaattgga aaagctgcct ttccgagaaa aagatgtttt gaagggattg
     9421 aagaaatctc gatggccagg aagaatggag cgtttgagta acgagccatt agttgtttta
     9481 gatggtgccc ataatacgca tgcaatgaac cggcttgttg agaatttgaa aaaagaattc
     9541 aaaggatatc aaatcaacat tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg
     9601 ctagaacgat tgcagcaagt accaaatgtc catatttatt tgacgacctt tgaacatcca
     9661 aaagccatcg aattaagtga agtatcagat ttagcaagtg agagagtatc aatcgtttca
     9721 ttgtggcaat tcggattagc agatatattg gaaaagatga caaatgaaga cgtattagtt
     9781 gtgaccggtt cactttattt catttcagaa gttcgccatt tattaataca attaggagga
     9841 gatcatgaag tataatgcag tgatttttga catggatggt ttgctgttcg acacagagtt
     9901 agtgtattac gaagccacac aagtagtagc agacaagatg ggttttcctt atgataaaga
     9961 cctgtattta aaattcttag gtgtttcaga tgaagaagta tgggcgaatt atcatatgat
    10021 tttctcggaa tttggcaaaa caagtgtaca aagattcata gatgattcat accaggaaac
    10081 gcttgaacgt ttttcgcaag gagcggtaca aatgaagccc ggtgtgttgg aactcctctc
    10141 ttttttagaa gaacatcaga ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat
    10201 tgaactttta ttgaagaaga acaatctgtt tgaacgattt gagacgattg tttctgcaga
    10261 aaatgttcaa cgagcaaaac cagacccaga aattttcttg ttagctaatc agaagctagg
    10321 tacagcgaag aaagaaacat tgattttaga agattcacaa aatggtattt tagcagcttc
    10381 tgcggctgag atcccggtag tcatggtacc agatctgctt ccaccatccc aagaattgat
    10441 caaaaaaaca gctggggttg tttcttcttt acatgaaatc cctgcattgt taaaataaaa
    10501 atccatctct tttcgtatct tattttagat aagatagaaa ggagatgttt ttttgggaaa
    10561 acgattgatc aaagaagtac ctactagctc tttaccaagg gaacgtatgg aaatttatgg
    10621 cgcggaagct ttatcggatc aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc
    10681 ttatagtgtg atgtcgattg ctggaaatct gttaaaaacg tttggaggac tggcctcttt
    10741 acgacaggct actttgcatg agttagaaga gattcgaggg ataggacgcg tgaaagcaat
    10801 cgaaatcaaa gccttgatcg aattaggtcg acgaattcag acggatcatg aaagagcggc
    10861 tccaccggtt cgtaccagct acgaattggc ccagcaattg attctggaaa tgaaagatca
    10921 caaacaagaa catttggttt gtatttattt ggacacgaag aatcaagtga tcttaaaaaa
    10981 aacagtgttc atcggttcgc tgaatcagtc agtcgctcat cctagagaaa ttttccatta
    11041 tgctgtacgt tattgtgcag caaggatcgt tttagcgcat aatcatcctt ctggtaatgt
    11101 tattccatcg cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat
    11161 ggggatcact gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga
    11221 agaaggaatg atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac
    11281 aattatattg taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta
    11341 cacgagcaat gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa
    11401 aacggaacag taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat
    11461 ggaagcgacg tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa
    11521 gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac
    11581 gttgtaaaag cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag
    11641 agaacaaagc gagtaacaaa aatacctgcc acaagagaca atttctcttg tggcaggtat
    11701 ttttttgtat gaaaaagtaa ataaatttaa gaggctatga aagagccacc ttgtatcaag
    11761 gaataagaac aaacaaaaat ggcttctcat gtttttcatt tgttttctct tatattcagg
    11821 acaaggtgct gatttcataa cctcttattt attcttcatc tggtgtcatt accattggca
    11881 aaatcattgg atgtctttct gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg
    11941 cttctttcaa tttatattct gaacagtcag gtccattcaa acattctctt aaagcgtgga
    12001 ataagattcg ctgagcttca ttgatcattt ctcctgattc tcgcatgtag acaaatccac
    12061 gagagagaat atctggacca gccaagattt cttttttctt gatatccact gtcgcgacag
    12121 ctaatacaag accttcttct gaaagcaggt gacggtcacg taaaacgaca ttaccaatat
    12181 caccgatccc gctaccatcg atatacacat ctccagccac aaaatgccca gcaggacgcg
    12241 cagaatcgct agttaacgct aaaacatcgc cattttccat gatgaagcag ttttctgcag
    12301 gaacacctac atcttgtgct agagaagcat gtactttcaa catgcggaat tctccgtgga
    12361 caggaataaa atattttggc ttcatcaaac gaagcattaa tttttgttct tcttgtccac
    12421 cgtgaccgga agtatggatg ttattgattt ttccgtgaat cacttctgca ccagcttcag
    12481 acagcaaatt gatcaaatga ttgacacttg ttgtattacc aggaataggt gaacttgaaa
    12541 agatcactgt atcgtctggc tggatttgga tttgacggtg agtaccattc gcgatgcggc
    12601 tcaacgcggc catcgcttct ccttgagaac ctgtacatag gatcatcgtt tcattggcag
    12661 gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc tggaactttg atatatccta
    12721 aacgttgacc gttgacgatg gcattttcca tgcttcgtcc gaaaacagcg attttacgac
    12781 cagttgccac agcggcatct gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg
    12841 caaaaatgat acgtccttcg atttttgaaa agatcttcaa aatagaatgc ccaatcgttt
    12901 tttctgattt agtaaatgtt ggaatctcag catttgtact atcagataaa aggcacaaga
    12961 ctccttcttc cccgatacga gccattttat gcaagtttgc tggttcgcca acaggagtga
    13021 aatcgaattt gaagtctcca gtcgctacga tatttccaga aggtgttttc accacgatcc
    13081 ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac
    13141 ggatgatcgt atcttcattg atctcatgga gctctgcatc tcgaagcaag ccatgctcat
    13201 ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc atagatagga atatttgctt
    13261 gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg
    13321 cttttacttt gtgtaagttt tgtatgatgt agctgtagtc gggaatgaca tagtcaattc
    13381 caagtaaatc gtcttccggg aatttgatcc cggcatcaat caaaatgatt tcatcttgga
    13441 attgtacccc atacgtattt ttcccgattt cacctaatcc accaatagca aagacgcctg
    13501 tctcgttatt tttgatggaa agtttcatat taaaactcca ctaatgagaa atcagcattt
    13561 tctttttcgt actctagatg acttccttct agaagttgaa cgaattcaat attgtatggg
    13621 gtattttctt ccacacgttg gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat
    13681 tttgtttctt cccgtttagg gttgcgtact tttgtttctt gataataaac tttatagatc
    13741 attgttttgt ctcctttatc tgcgtaaagc agtgatttag ctagttgatt tttttagaca
    13801 acacaaaacg aggggacaag ccacctggtt tttcatatgc tttgactaga atactggatt
    13861 cgagtcaaag agacaagcct cgtttttgct tatctgttcg gatatttttt caaccgtacc
    13921 ttctgtataa aaaatataca acaattaatt gttattctag cataaaacag caaataagta
    13981 tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc atcccgctat aatcataatg
    14041 acttttttat gagagaagtg ttctattaat aggctcgttg tgctatcatt tttagtaatg
    14101 aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa ctaatgtggc attataccat
    14161 gcgttataag aaattcttgt ttttcaattt catatgcgta tttggtttta ttttgattga
    14221 attaggactt cctacgattt tagcacgtat gatcgatgta ggaatccgca atgatgattt
    14281 tgattatgtg aagcaacaag ggatatttat gatcgttatt actgtaatcg gtattatttt
    14341 gaatatcttg ttaggttatt ttggttcgag gattacgact aatatcgtag cagatattcg
    14401 agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt
    14461 atcttcattg atcactcgta caacaaacga tgcttatcag atcatgttgt tcctgcaaaa
    14521 tattttacgt atcggtttta tggcaccaat gatgttcatt gtcagtttgt acatggtcat
    14581 gcgtacaagt gtgacactca gtttgtatgt agtaggtgca ttacctttac tattattggc
    14641 agtcgtagcg attgctaagg tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga
    14701 taaaatcaac agtatcttgc gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt
    14761 caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat gagaattatg cttctagttc
    14821 aaaaagctta ttccgtttga tggctgttgc acaaccgggt ttttatttct tatttaatat
    14881 cgtaatggta ttgatcatct ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt
    14941 aggagatttg attgcgttta tcgagtatat attccatgcc ttgttctcat tcatgttatt
    15001 tgctagtgtg ttcatgatgt atccaagagc agctgtttca gctggacgta tccaagaagc
    15061 cctagatgcc aaaccttcga ttgtagaaga tccaaatggt atatctgata caaaaactaa
    15121 agggtatatc gaatttcaaa atgtaacatt tgcttatcca ggccattcgc aagagccagt
    15181 gatacgaaat gtcagtttta cagcctctcc aggagagacc gttgctttta ttggaagtac
    15241 cggtagcgga aaatctacgt tgatccaatt gatccctcgc ttttatgatg tgagtgaagg
    15301 aaagatcttg atcgatggag tagacgtgag agattatcgg ctaagcaagt tgcgagataa
    15361 gattgggttt attccgcaaa aagcgctgct atttactgga acgattgcag ataatttgcg
    15421 atacgggaaa gaagatgcta cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc
    15481 ttcagaattc atttctcgaa aaccagatgg atttaatgaa catttatcag aaggtggttc
    15541 caatttttca ggtggacaaa aacaacgatt ggcaatagcc agagccatca tacgccgtcc
    15601 ggaaatctat atttttgatg acagcttttc tgcattagat tatcaaacgg atgcgaagtt
    15661 acgtgctcgt ctgaaaaaag aaacgacaga atctactgta ttgatcgtag cacaacgtgt
    15721 gggaacgatc atgcatgcag ataagatcat cgtattaaat gaaggagaag tcgtcggtat
    15781 ggggacgcat cgggagttgt tggaaaaatg cccgatttat tatgatattg ccgcttctca
    15841 attatcaaag gaggaattag catgaaaaaa accttttctt ccttaaaacg tttagctcga
    15901 tatatcagac cgtatcgtga gacttttctt ttagtgatcc tatttacagt tctgacagtg
    15961 gctttcaacg tggctttgcc atatgttaca ggacttccta ctacagaaat cagccgtaat
    16021 attgccaatg gcgaatcaat caatttttct tatatattca aatgtctcat ttggatcaca
    16081 gttgttggaa tagggtattg tatttcgcaa ttactttctg gtgttttgat gacaaatgtc
    16141 gttcaaagtg ccatgcatga tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg
    16201 gtttcatatt tcgataaaaa ccagcaagga aatattttgt ctcgtgtgac gaatgatgta
    16261 gatgcagtca gcggtgcttt acaacaagct tttattggta tcgtaaatgc gatattggga
    16321 attacgttgg ctgcagcaat gatgttttat atccagccga tgatggcgct gatctctatg
    16381 atcatgattc caacatcgat ctggatctca aaacgagtga tcaatgcttc gcagaaatat
    16441 tttcaaagca tgcagaattc tcttggtgag ctaaacggct acgtccaaga aaatatgaca
    16501 ggttttagtg tattgaaagt atatggacgc gaaaaagaaa cattggaagg attcaaaaaa
    16561 gttaatcaca gcttgaaata ctttgggttt cgtgcagcat tcatttctgg cttgatgatg
    16621 cctctagttc aattaactgc ctatgctact tatattggta tggctgttct aggaagcttt
    16681 tatgttatta ctggggcgat cgttgtcggt caactacaag cattcatcca atatatttgg
    16741 caaatcagtc agccaatggg gaacgtgaca caattgtctt cagttttgca aagtgcatca
    16801 gcggcaacga aacgtgtatt cgagatttta gatgagccag aagaaaaaga aaacaaagta
    16861 gatgcttcac tgccagaaac gattttagga gatgtgacat tcgatcatgt cgattttgca
    16921 tatgatccga agaaaccatt aatccaagat ttgaattttg aagtgaaagc tggtcaaacc
    16981 gtagctgtgg tcggtccgac cggtgccggc aagacgacat tgatcaatct attgatgcgt
    17041 ttttatgacg tagacaaagg agcaataagg attgatggaa tcgacaccaa atcaatgagt
    17101 cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag atgcatggct ttatcaagga
    17161 acgattgcag ataatatccg cttcggaaag cttgatgcga cagattatga agtagttgat
    17221 gctgcgaaaa cagcaaacgt agatcatttt atccgaacga tgccagatgg atatgatatg
    17281 gccatcaatt ctgagggaga caatgtgtct ttaggacaaa aacagttgct gacgattgcc
    17341 cgagcagtgg tctccgatcc taaaatcttg attttagatg aagcaacaag ttcagtagac
    17401 acccgtttag aagcgttgat tcaaaaagca atggatcgcg tgatggaggg acggacaagt
    17461 ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag atctgattct agtgatggat
    17521 caaggtcaga tcattgaaaa aggaacgcat gagagtttat tggcacaagg tggattttat
    17581 gaaaaactgt ataacagcca atttgctgag gaagcagaat aagtcatata agaataaaac
    17641 agtttaaaac ttattttttg ttctctattc ttgataatgg taaagtaaag ttatcaagaa
    17701 aagtgaggac acggagatgg aacagttttt aaatgaaaaa attactgaag ccaaaagaga
    17761 agttgatcgt ttaacgcacg tctgtcagac gaagcctttt gattcagctg atttttttga
    17821 aaatgagttt caaatcgaac gcttggcagc acaaatagaa gtgtacgaat taattttaaa
    17881 aaaagtaata gaagaaaata tagatgtaca ataaaaacag gtaaaacgtc agttaccaga
    17941 tactgtaaat agacatctgg cctgagcagg ttttacctgt tttttttctt atgaaataaa
    18001 tatatgctat gataccttta ttcaatagga ttagtttcta caaaaaatga aaggagaaaa
    18061 ataaatatgg aacggtttat agcatcaaat gccacagtga ttggtgatgt gacattgtca
    18121 gaagatgtga cgatctggta tcaagctgtt ttacgcgggg acagtaattg gataaaaata
    18181 ggacaaagaa cgaatatcca agatggtaca atcatccatg tcgatcatga tgcacctgta
    18241 gatattgcgg agaatgtgac ggtaggacat cagtgcatgc tacatggctg tacaatcgaa
    18301 aaaggggcgc tgatcgggat ggggaccacg atattgaatc atgcggttat tggtgagaat
    18361 agtttgattg gtgctggatc attagtaaca gaaggaaaag ttattcctcc taatgtcttg
    18421 gcttttggtc gcccagctaa agtgatccgt cctttgacaa aagaagaaat acaaaaaaac
    18481 aaagaaaaca tccagcatta tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag
    18541 gagcaaagat aggcagtagc atccataaaa taaaaaagga tggtcagttt gagttgacca
    18601 tcctttttta tttggtttag ataagagagc ttaaaaaagc ttcctttgaa acagattaat
    18661 ttaatgaaga actgttttcc atgacttcat cgatcaatcc atatgctttt gcttgttcag
    18721 ctgtcatata gttgtcacga tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc
    18781 gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt
    18841 cgatttcagt tgcttgccct tgagcgccgc caagaggttg atggatcatg atctcagcat
    18901 ttggtaaagc aaatcgttta ccttttgtac cagctgtcag caagaagctt cccattgaag
    18961 cagccatacc cataacaatt gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa
    19021 tagccatacc tgctgtaact gaaccaccag gagaattgat ataaagatag atatcttttt
    19081 cagaatcttg agcgtctaag aaaagcaatt gggcgatgat cgaattagca agatcatctg
    19141 ttacttggcc gcttaacatg atgatacggt ctttcaataa acgtgagtag atatcgtaag
    19201 ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat taaattcata atagagttcc
    19261 tccttgattt cgtttagaaa tagtgtacca taatttagtt tctaatacct aaaatgtagt
    19321 atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg caaattagtc gcataaaccc
    19381 gctttatggt atgataagca gagaaacaaa acaaggagga acaacagatt gatagttggt
    19441 attactggcc ttatcggagt gctgacaggt gctgtagttg tctatatcgg catgatactg
    19501 caaatgcgtc acttggaaaa taaagaacag cgtagacgtg aattggaatt gaaagtcaaa
    19561 gaaatcgaga cgctgaatca attaaataaa aagatcaacg agatcttgca aaaacggaca
    19621 acgctgatgg aagaatatgt cagctttgat gcatttgatg attgttatat cacgattgac
    19681 gattttgcgt atctccaatc attttcagcg caaaatagtt tttatctgcc aaattatatt
    19741 ttagacgagc tatttaagaa tatctcccat cgaaaagtga ttttatctcc agatgaaaca
    19801 atgaagattg gtggatatgc ttataaaggc ggacgcgtga ttttagagaa ttttacggat
    19861 agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga agactatgac gcgccagccg
    19921 attggtttct tctcgaaaga tgatgtttgg agctgatatt ttgaagcatt gccgttgcac
    19981 atgagagcgt ggtaatgctt tctttttttc ttaatgatac aatagaactg aacggagtga
    20041 agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg
    20101 cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg gtatcgaact agatgtccat
    20161 ctaagcaaag atggttattt gatcgttatg cacgacgaag aagtggaccg gacgactaac
    20221 ggtaaagggc tgattcgaga aaaaacattg gaagaactca aaaaattgaa tgcagggggc
    20281 aaattcagca aagaatttcc agcagctaaa gttcctgaat tgagagaagt gttaggactg
    20341 ctcctccaaa aaaattaccg agggatgcta atgattgaaa ttaaaactga tcagtatgaa
    20401 tatcccggta tcgaagaaaa attagccgaa acattaaaaa gtagagattg gccttttcaa
    20461 catgcatatt gcagctttca tttggattct ttggaaaaaa tcgcattact ggaaccggag
    20521 atacagctgg acttattgat gagtacttct gataaaaaga tccaactagc agcagaaacc
    20581 ccttatatag aaggagtaca tccaaaaaat acttggttgt tcgaacatcc agtccaggca
    20641 gctgattttc caaaagcaat tcgtgcatgg acgattaacg atgaagaaga aatggccaaa
    20701 tgtcaagaac tcaaaataga tggaataata actgattttc cggagaaagc tcttcggtgg
    20761 aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca
    20821 gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac tatttggagg agagatcatc
    20881 agtggtgatt cattgcaaat ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa
    20941 gaagagatga ataatgttcc gcatcatttg attgatgtga tcgaacctac tcaacgttac
    21001 tcagccgttg attttcagca ttcaggtcga caattgatta ccgagattgc gaaccgaggg
    21061 cacctaccta tcatagctgg tggtacgggt ctctatatcc aatcccttct atatgattat
    21121 cagttaggag cgacagaaga agcgaatcct gagattagaa aaaagtatga aacgttagct
    21181 gaaacaattg gttcccaagc attatggaat cagttgaaag aaaaagaccc ggcagctgct
    21241 gagaaaatcc actggaacaa tcaacgcaaa gtgataagag ctctggaagt tttagagacg
    21301 acaggacata gtatcacggc acctaaagaa gagccaaagc aattgtatga ttacctcatg
    21361 attggattag atacgaagcg tagtattctt tatgaacgaa tcaatcaaag agtcgatttg
    21421 atgctagcag aaggtttagt agcagaagcg cgtaaggtgt atgagcttgg agaagtccag
    21481 gccagtcaag gtattggata taaagaattt ttcccttatt ttgaagggaa agaaacattg
    21541 gaagaagcaa cagaacaagt caaactacat tcgagaagat acgcgaaacg tcagttgacg
    21601 tggtttcgta accgtttgga tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa
    21661 agtcagattg agcaaacaat caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt
    21721 gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac ttttcagagt caatggaaga
    21781 gctagttcag ctgacgaata ccgcatcagg agaagtcgta ttcacaatta cacaaaagag
    21841 accacaagtg gatcggcaaa cgattatcgg taaagggaaa ttacaagaat tagttcaaca
    21901 agcggatgcg catgaagcgg atttgatcat ctttaatcat gagctaactc cgagacagag
    21961 ccaactgatt accgatgctg ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga
    22021 tatttttgcg atgcgtgctc gttcgaaaga agggaagctg caggttgaac ttgctcagtt
    22081 agaatacctg ctgcctcgtt tagtagggca agggaaaaca ttatctcgtc ttggcggtgg
    22141 gatcggaaca agagggccag gtgaaacaaa gttagaaaca gaccgccgac atatacggaa
    22201 taaaattttt gctgttaaga aagaattgaa agaagtagaa gctcatcgag agcgcaatcg
    22261 ccaaaaaaga aaaaatagcg agatttttca gattggactt attggctaca cgaatgccgg
    22321 gaagtcaact atcttgaatc tattgacaca agcggatact tattcgaaag atcaattgtt
    22381 tgcaacgtta gatccattga ctaagagatg gcgttttgct gaaggatttg agattactgt
    22441 aacggatacc gtcgggttca tccaagacct gcctacgcaa ctgatcgatg ctttccattc
    22501 tacattagaa gaaagtcaaa atatggatct tttgctacat gtagtcgatg ctagttctcc
    22561 agatcgtatt ttacaggagc agactgtctt aaaattgatg gatgaactga atatgaaaga
    22621 aatgccgata ctgactgttt acaataaggc agaccagatc gacccagcga tgttcacgcc
    22681 tacgctattc ccaaatgtat tgatttctac tcagacgaaa gaaggtaaga tggcgttgat
    22741 cgaagcaatc aaacgtcagc tgatggaact aatgacccct tatacacttt ctgtgccaag
    22801 caacgaagga cagaaattaa gcgagttgag aagacaaaca atggttttgg acgaagcgta
    22861 catggaagaa acaaacgaat atgaagttaa agggtttgca aaaaccaatt caaaatggtt
    22921 aagaaaaaac caataaaatt tt
//
LOCUS       DADQRX010000042        22419 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.042, whole genome shotgun sequence.
ACCESSION   DADQRX010000042 DADQRX010000000
VERSION     DADQRX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.042"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="J8V14_001512"
     CDS             complement(<1..1835)
                     /locus_tag="J8V14_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921624.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="J8V14_001513"
     CDS             complement(1832..2536)
                     /locus_tag="J8V14_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA0921625.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="J8V14_001514"
     CDS             complement(2548..4857)
                     /locus_tag="J8V14_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA0921626.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="J8V14_001515"
     CDS             complement(5052..5516)
                     /locus_tag="J8V14_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921627.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="J8V14_001516"
     CDS             complement(5516..6142)
                     /locus_tag="J8V14_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA0921628.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="J8V14_001517"
     CDS             complement(6149..6514)
                     /locus_tag="J8V14_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBA0921629.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="J8V14_001518"
     CDS             complement(6504..6842)
                     /locus_tag="J8V14_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921630.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="J8V14_001519"
     CDS             complement(6832..7158)
                     /locus_tag="J8V14_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBA0921631.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="J8V14_001520"
     CDS             complement(7136..7417)
                     /locus_tag="J8V14_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBA0921632.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="J8V14_001521"
     CDS             complement(7419..8783)
                     /locus_tag="J8V14_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBA0921633.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9371)
                     /locus_tag="J8V14_001522"
     CDS             complement(8796..9371)
                     /locus_tag="J8V14_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBA0921634.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="J8V14_001523"
     CDS             complement(9337..10524)
                     /locus_tag="J8V14_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA0921635.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="J8V14_001524"
     CDS             complement(10588..12315)
                     /locus_tag="J8V14_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBA0921636.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="J8V14_001525"
     CDS             complement(12312..12764)
                     /locus_tag="J8V14_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HBA0921637.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="J8V14_001526"
     CDS             complement(12876..13256)
                     /locus_tag="J8V14_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA0921638.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="J8V14_001527"
     CDS             complement(13253..13639)
                     /locus_tag="J8V14_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921639.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="J8V14_001528"
     CDS             complement(13641..13919)
                     /locus_tag="J8V14_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921640.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="J8V14_001529"
     CDS             13967..14695
                     /locus_tag="J8V14_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBA0921641.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="J8V14_001530"
     CDS             complement(14841..15317)
                     /locus_tag="J8V14_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBA0921642.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="J8V14_001531"
     CDS             complement(15277..15624)
                     /locus_tag="J8V14_001531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921643.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="J8V14_001532"
     CDS             complement(15611..15796)
                     /locus_tag="J8V14_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921644.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="J8V14_001533"
     CDS             complement(15797..16069)
                     /locus_tag="J8V14_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBA0921645.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="J8V14_001534"
     CDS             complement(16066..16269)
                     /locus_tag="J8V14_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921646.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="J8V14_001535"
     CDS             16394..16606
                     /locus_tag="J8V14_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921647.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="J8V14_001536"
     CDS             complement(16639..16833)
                     /locus_tag="J8V14_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921648.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="J8V14_001537"
     CDS             complement(16846..17028)
                     /locus_tag="J8V14_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921649.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17655..18020)
                     /locus_tag="J8V14_001538"
     CDS             complement(17655..18020)
                     /locus_tag="J8V14_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921650.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="J8V14_001539"
     CDS             complement(18060..18362)
                     /locus_tag="J8V14_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921651.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="J8V14_001540"
     CDS             complement(18362..18661)
                     /locus_tag="J8V14_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921652.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18824..19093)
                     /locus_tag="J8V14_001541"
     CDS             complement(18824..19093)
                     /locus_tag="J8V14_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921653.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="J8V14_001542"
     CDS             complement(19105..19854)
                     /locus_tag="J8V14_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921654.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="J8V14_001543"
     CDS             complement(19851..20543)
                     /locus_tag="J8V14_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921655.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="J8V14_001544"
     CDS             complement(20549..21220)
                     /locus_tag="J8V14_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBA0921656.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="J8V14_001545"
     CDS             complement(21213..21554)
                     /locus_tag="J8V14_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921657.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22415)
                     /locus_tag="J8V14_001546"
     CDS             complement(21732..22415)
                     /locus_tag="J8V14_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBA0921658.1"
                     /translation="MLKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFV
                     EGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVP
                     QTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAY
                     EEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQ
                     QRLFGDDNE"
BASE COUNT         6190 a         4742 c         3485 g         8002 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattact
//
LOCUS       DADQRX010000043        22170 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.043, whole genome shotgun sequence.
ACCESSION   DADQRX010000043 DADQRX010000000
VERSION     DADQRX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.043"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..329)
                     /locus_tag="J8V14_001547"
     CDS             complement(<1..329)
                     /locus_tag="J8V14_001547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921659.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..573)
                     /locus_tag="J8V14_001548"
     CDS             complement(343..573)
                     /locus_tag="J8V14_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921660.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="J8V14_001549"
     CDS             complement(795..1337)
                     /locus_tag="J8V14_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921661.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="J8V14_001550"
     CDS             complement(1358..2449)
                     /locus_tag="J8V14_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBA0921662.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="J8V14_001551"
     CDS             complement(2457..4667)
                     /locus_tag="J8V14_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921663.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="J8V14_001552"
     CDS             complement(4683..7211)
                     /locus_tag="J8V14_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBA0921664.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="J8V14_001553"
     CDS             complement(7263..7673)
                     /locus_tag="J8V14_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921665.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="J8V14_001554"
     CDS             complement(7686..7904)
                     /locus_tag="J8V14_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921666.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="J8V14_001555"
     CDS             complement(7901..8893)
                     /locus_tag="J8V14_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0921667.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="J8V14_001556"
     CDS             complement(8972..9883)
                     /locus_tag="J8V14_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBA0921668.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="J8V14_001557"
     CDS             complement(9902..10246)
                     /locus_tag="J8V14_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921669.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10822)
                     /locus_tag="J8V14_001558"
                     /pseudo
     CDS             complement(10266..10822)
                     /locus_tag="J8V14_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10903..11403)
                     /locus_tag="J8V14_001559"
     CDS             complement(10903..11403)
                     /locus_tag="J8V14_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921670.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="J8V14_001560"
     CDS             complement(11404..11949)
                     /locus_tag="J8V14_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921671.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="J8V14_001561"
     CDS             complement(11946..13202)
                     /locus_tag="J8V14_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBA0921672.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="J8V14_001562"
     CDS             complement(13563..13874)
                     /locus_tag="J8V14_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921673.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15207)
                     /locus_tag="J8V14_001563"
     CDS             complement(13897..15207)
                     /locus_tag="J8V14_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBA0921674.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="J8V14_001564"
     CDS             complement(15821..16273)
                     /locus_tag="J8V14_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921675.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="J8V14_001565"
     CDS             complement(16275..16640)
                     /locus_tag="J8V14_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921676.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="J8V14_001566"
     CDS             complement(16739..21022)
                     /locus_tag="J8V14_001566"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBA0921677.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="J8V14_001567"
     CDS             complement(21169..21408)
                     /locus_tag="J8V14_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921678.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21770)
                     /locus_tag="J8V14_001568"
     CDS             complement(21498..21770)
                     /locus_tag="J8V14_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921679.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21853..22104)
                     /locus_tag="J8V14_001569"
     CDS             complement(21853..22104)
                     /locus_tag="J8V14_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921680.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6478 a         4804 c         3308 g         7580 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa
//
LOCUS       DADQRX010000044        21833 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.044, whole genome shotgun sequence.
ACCESSION   DADQRX010000044 DADQRX010000000
VERSION     DADQRX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.044"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="J8V14_001570"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="J8V14_001570"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA0921681.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="J8V14_001571"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="J8V14_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBA0921682.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="J8V14_001572"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="J8V14_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBA0921683.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="J8V14_001573"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="J8V14_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBA0921684.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="J8V14_001574"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="J8V14_001574"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBA0921685.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="J8V14_001575"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="J8V14_001575"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBA0921686.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="J8V14_001576"
     CDS             complement(8054..8524)
                     /locus_tag="J8V14_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBA0921687.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="J8V14_001577"
     CDS             complement(8570..8995)
                     /locus_tag="J8V14_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBA0921688.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="J8V14_001578"
     CDS             complement(9062..9331)
                     /locus_tag="J8V14_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBA0921689.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="J8V14_001579"
     CDS             complement(9342..10937)
                     /locus_tag="J8V14_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBA0921690.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="J8V14_001580"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="J8V14_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBA0921691.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="J8V14_001581"
     CDS             complement(14490..15050)
                     /locus_tag="J8V14_001581"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA0921692.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="J8V14_001582"
     CDS             15403..16377
                     /locus_tag="J8V14_001582"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBA0921693.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="J8V14_001583"
     CDS             16547..17947
                     /locus_tag="J8V14_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HBA0921694.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="J8V14_001584"
     CDS             complement(18025..18747)
                     /locus_tag="J8V14_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA0921695.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="J8V14_001585"
     CDS             complement(18826..19332)
                     /locus_tag="J8V14_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBA0921696.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="J8V14_001586"
     CDS             19455..21254
                     /locus_tag="J8V14_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBA0921697.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..>21833)
                     /locus_tag="J8V14_001587"
     CDS             complement(21322..>21833)
                     /locus_tag="J8V14_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBA0921698.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6049 a         4791 c         3964 g         7029 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttg
//
LOCUS       DADQRX010000045        21475 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.046, whole genome shotgun sequence.
ACCESSION   DADQRX010000045 DADQRX010000000
VERSION     DADQRX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.046"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            854..2023
                     /locus_tag="J8V14_001588"
     CDS             854..2023
                     /locus_tag="J8V14_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBA0921699.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2496..3050
                     /locus_tag="J8V14_001589"
     CDS             2496..3050
                     /locus_tag="J8V14_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA0921700.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(3223..3696)
                     /locus_tag="J8V14_001590"
     CDS             complement(3223..3696)
                     /locus_tag="J8V14_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA0921701.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3708..4244
                     /locus_tag="J8V14_001591"
     CDS             3708..4244
                     /locus_tag="J8V14_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBA0921702.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            4284..4655
                     /locus_tag="J8V14_001592"
     CDS             4284..4655
                     /locus_tag="J8V14_001592"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBA0921703.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            4939..6210
                     /locus_tag="J8V14_001593"
     CDS             4939..6210
                     /locus_tag="J8V14_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBA0921704.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            6455..7480
                     /locus_tag="J8V14_001594"
     CDS             6455..7480
                     /locus_tag="J8V14_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0921705.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7774..8883
                     /locus_tag="J8V14_001595"
     CDS             7774..8883
                     /locus_tag="J8V14_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA0921706.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            8966..9862
                     /locus_tag="J8V14_001596"
                     /pseudo
     CDS             8966..9862
                     /locus_tag="J8V14_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            9878..10702
                     /locus_tag="J8V14_001597"
     CDS             9878..10702
                     /locus_tag="J8V14_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921707.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10734..12044
                     /locus_tag="J8V14_001598"
     CDS             10734..12044
                     /locus_tag="J8V14_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBA0921708.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12192..13451
                     /locus_tag="J8V14_001599"
     CDS             12192..13451
                     /locus_tag="J8V14_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBA0921709.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13461..14333
                     /locus_tag="J8V14_001600"
     CDS             13461..14333
                     /locus_tag="J8V14_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921710.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14345..15175
                     /locus_tag="J8V14_001601"
     CDS             14345..15175
                     /locus_tag="J8V14_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921711.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            15215..17398
                     /locus_tag="J8V14_001602"
     CDS             15215..17398
                     /locus_tag="J8V14_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBA0921712.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17459..18679
                     /locus_tag="J8V14_001603"
     CDS             17459..18679
                     /locus_tag="J8V14_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBA0921713.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18676..20136
                     /gene="gtfA"
                     /locus_tag="J8V14_001604"
     CDS             18676..20136
                     /gene="gtfA"
                     /locus_tag="J8V14_001604"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBA0921714.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            20213..21319
                     /locus_tag="J8V14_001605"
                     /pseudo
     CDS             20213..21319
                     /locus_tag="J8V14_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         7359 a         3237 c         4064 g         6815 t
ORIGIN      
        1 aattttctac gttttccttt tcatatatat taaatattac ggaaaattat ataaactaat
       61 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
      121 taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt
      181 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
      241 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
      301 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
      361 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
      421 ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt gataataaag
      481 tactacctat taaaggtagt actttattta atttagtata aattaacata ctactatatt
      541 ttaggtttca tattatataa attaataata tactaccatt tgttggtagt ataatttaaa
      601 ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag
      661 agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat
      721 aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta
      781 gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata
      841 ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg
      901 tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc
      961 tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa
     1021 ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt
     1081 aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac
     1141 caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga
     1201 aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa
     1261 tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag
     1321 atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa
     1381 agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt
     1441 tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga
     1501 ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag
     1561 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
     1621 tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt
     1681 ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg
     1741 gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta
     1801 taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa
     1861 caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga
     1921 gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa
     1981 aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg
     2041 gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt
     2101 tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg ttattcatat
     2161 gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa acttaaattt
     2221 catataattt aattatgtaa attagctatt ctaagaactt ctacggataa ctgcccgtct
     2281 aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt tgtacaaaaa
     2341 agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc ccttgatttt
     2401 attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat atacataatc
     2461 attaactcag aatattttta agaagggaaa caatagtgag ctataatgta caaccgttaa
     2521 ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt aatccggaaa
     2581 gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca gatattatag
     2641 gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa cataaaacag
     2701 gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta tatactgaag
     2761 gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat ataacaaggg
     2821 atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat gatataggaa
     2881 cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga gacattgcta
     2941 ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac attggtataa
     3001 ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa tttaaaaatt
     3061 tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat tttatttcat
     3121 aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt gttatataaa
     3181 ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa agagaatcaa
     3241 tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact caacgtttag
     3301 cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt tgaccttctg
     3361 caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc
     3421 ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt taatatctgt
     3481 atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga gcagactaca
     3541 atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt taatgatgat
     3601 tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg atgaaaacaa
     3661 aagttgttca ccatctttta taggtttgat tctcattttt agatattatg cccgcacgta
     3721 ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt tatcataata
     3781 tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc
     3841 tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt gaagtacaag
     3901 aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta aaagttcctt
     3961 ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt tgccattatg
     4021 gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat gcaacacgat
     4081 tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg tatgtcacag
     4141 accagatccc tacagagcat gccattttag gaattgatgc cattcaacta cgcgtacaat
     4201 attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa gaatacgtca
     4261 gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact cagttctatc
     4321 aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg attggtagcg
     4381 tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc gacaaacaat
     4441 tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt gtatcacttt
     4501 attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga aacctagatg
     4561 caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt ttaccgcagt
     4621 acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc catgacgaag
     4681 agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag
     4741 aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta ataaaactac
     4801 ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt aaactaaaca
     4861 tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat aaaggggagt
     4921 accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa aaatgtcagt
     4981 ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc aggaaaatca
     5041 tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga cacctatgat
     5101 agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga gattgataat
     5161 ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta
     5221 gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct
     5281 agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg ttctgtctgt
     5341 cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc ggctattgat
     5401 aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg aattgaggaa
     5461 caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc agaagatgtt
     5521 aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa
     5581 gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga tacgcatctt
     5641 tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt aaatacaaat
     5701 gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca tgagaaaata
     5761 gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga acgacatcaa
     5821 gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg gatatataac
     5881 aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat tgttgttact
     5941 ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg
     6001 gaacaattat cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg
     6061 ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt
     6121 ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt
     6181 tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca
     6241 ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt
     6301 cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat
     6361 gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga ttatatgtta
     6421 gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa
     6481 acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc
     6541 agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa
     6601 aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt
     6661 agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga
     6721 acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga
     6781 cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga
     6841 agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga
     6901 agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta
     6961 taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca
     7021 aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa
     7081 acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg
     7141 aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat
     7201 catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct
     7261 acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt
     7321 aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg
     7381 catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa
     7441 taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa
     7501 gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt
     7561 aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg
     7621 aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca
     7681 gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt
     7741 tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga aaaacaaaat
     7801 cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa
     7861 gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat
     7921 aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt
     7981 gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga cgaactatac
     8041 gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat
     8101 gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt
     8161 gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact
     8221 ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa
     8281 tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt
     8341 gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt
     8401 caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa
     8461 aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca
     8521 acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc agaagaagtg
     8581 aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg
     8641 gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt
     8701 gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat
     8761 ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta
     8821 actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat
     8881 taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg
     8941 gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga
     9001 tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa
     9061 tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga
     9121 tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca
     9181 gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc
     9241 cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat
     9301 ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt
     9361 caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc
     9421 atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc
     9481 tattggttat aacatggtgt tattactagc cggcttataa gaaattccta aagattatta
     9541 tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc
     9601 attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt
     9661 atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc
     9721 attagcctat ttattctata aacattcatt tattttaaat gacaagggat atggttcggc
     9781 aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca
     9841 gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa
     9901 tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac cttttatttg
     9961 gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt
    10021 ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc
    10081 tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg ttttattttc
    10141 agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag
    10201 cttagtactt attcaaatga tgattcccgg ttccattttt attactccac agtatttatt
    10261 agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag
    10321 tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg aattggagga
    10381 agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct
    10441 tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt
    10501 aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc
    10561 aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc
    10621 aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc
    10681 tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa
    10741 aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga
    10801 tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac
    10861 taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg
    10921 atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg
    10981 ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat
    11041 atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac
    11101 ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt
    11161 ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga
    11221 gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc
    11281 atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta
    11341 atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc
    11401 gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga
    11461 aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact
    11521 ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact
    11581 tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata
    11641 atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg gattctatta
    11701 cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact
    11761 taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa
    11821 cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc
    11881 aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt
    11941 atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg
    12001 atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag
    12061 taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa
    12121 cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg
    12181 aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa
    12241 cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag
    12301 agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg
    12361 aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa
    12421 ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta
    12481 tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt
    12541 tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca
    12601 taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg
    12661 ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc
    12721 aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc
    12781 aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct
    12841 cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag
    12901 atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata
    12961 cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc
    13021 cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg
    13081 aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt
    13141 ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc
    13201 agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc
    13261 aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt
    13321 tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca
    13381 atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg
    13441 acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca
    13501 tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag
    13561 gtgcttttta cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt
    13621 tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt
    13681 taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg
    13741 cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg
    13801 tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa
    13861 aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg
    13921 gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca
    13981 ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg
    14041 gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt
    14101 caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt
    14161 taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg
    14221 cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa
    14281 ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt
    14341 cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg
    14401 cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga
    14461 tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt
    14521 gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt
    14581 agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg
    14641 tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca
    14701 agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg
    14761 attaatctta ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt
    14821 tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca
    14881 ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat
    14941 tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg
    15001 tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa
    15061 cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta
    15121 tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat
    15181 aaattattct cattataaag gagttcataa acttatgatt atatttgaca atcacacaca
    15241 aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta
    15301 tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc
    15361 ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt
    15421 agacacatta ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca
    15481 taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat
    15541 tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga
    15601 agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt
    15661 ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag
    15721 tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc
    15781 actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa
    15841 aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa
    15901 tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt
    15961 taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac
    16021 acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc
    16081 atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca
    16141 gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt taaaagaacg
    16201 tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat
    16261 gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg
    16321 gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa
    16381 acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg
    16441 gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga
    16501 ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga
    16561 tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg
    16621 tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt
    16681 cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga
    16741 atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg
    16801 aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa
    16861 taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc
    16921 aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt
    16981 aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca
    17041 attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg
    17101 ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac
    17161 tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt
    17221 agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac
    17281 ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat
    17341 tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta
    17401 gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat
    17461 gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt
    17521 acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag
    17581 aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg
    17641 agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg
    17701 gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc
    17761 cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt
    17821 ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaagatag
    17881 ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga
    17941 taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata
    18001 tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt
    18061 actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat
    18121 ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga
    18181 atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc
    18241 tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt
    18301 atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca
    18361 acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa
    18421 agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct
    18481 ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga
    18541 acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt
    18601 tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa
    18661 ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt
    18721 ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt
    18781 ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt
    18841 gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa
    18901 gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc
    18961 caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat
    19021 gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa
    19081 gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat
    19141 acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt
    19201 cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt
    19261 gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa
    19321 cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc
    19381 catgagcatt attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc
    19441 gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat
    19501 tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt
    19561 gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg
    19621 tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat
    19681 atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg
    19741 ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta
    19801 ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat
    19861 cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa
    19921 ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt
    19981 gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt
    20041 gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat
    20101 ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc
    20161 tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa
    20221 gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc
    20281 tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga
    20341 agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt
    20401 gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca
    20461 tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca
    20521 acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct
    20581 gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca
    20641 aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta
    20701 tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt
    20761 ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat
    20821 ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt
    20881 tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt
    20941 tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa
    21001 gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc
    21061 tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat
    21121 gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac
    21181 gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt
    21241 gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga
    21301 aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga
    21361 ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga
    21421 cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca acact
//
LOCUS       DADQRX010000046        21309 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.047, whole genome shotgun sequence.
ACCESSION   DADQRX010000046 DADQRX010000000
VERSION     DADQRX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.047"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..808
                     /locus_tag="J8V14_001606"
     CDS             68..808
                     /locus_tag="J8V14_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBA0921715.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="J8V14_001607"
     CDS             complement(1232..1402)
                     /locus_tag="J8V14_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0921716.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="J8V14_001608"
     CDS             complement(1596..1775)
                     /locus_tag="J8V14_001608"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921717.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="J8V14_001609"
     CDS             2162..3274
                     /locus_tag="J8V14_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA0921718.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="J8V14_001610"
     CDS             complement(3352..3645)
                     /locus_tag="J8V14_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBA0921719.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="J8V14_001611"
     CDS             3813..4127
                     /locus_tag="J8V14_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBA0921720.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="J8V14_001612"
     CDS             4148..6127
                     /locus_tag="J8V14_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBA0921721.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="J8V14_001613"
     CDS             6140..6466
                     /locus_tag="J8V14_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBA0921722.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="J8V14_001614"
     CDS             6482..7000
                     /locus_tag="J8V14_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921723.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="J8V14_001615"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="J8V14_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBA0921724.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="J8V14_001616"
     CDS             complement(8436..9941)
                     /locus_tag="J8V14_001616"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBA0921725.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="J8V14_001617"
     CDS             complement(10033..10623)
                     /locus_tag="J8V14_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBA0921726.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="J8V14_001618"
     CDS             10802..12448
                     /locus_tag="J8V14_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBA0921727.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="J8V14_001619"
     CDS             12497..13216
                     /locus_tag="J8V14_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBA0921728.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="J8V14_001620"
     CDS             13695..14642
                     /locus_tag="J8V14_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921729.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="J8V14_001621"
     CDS             14654..15574
                     /locus_tag="J8V14_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921730.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="J8V14_001622"
     CDS             15615..17072
                     /locus_tag="J8V14_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0921731.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="J8V14_001623"
     CDS             17417..18031
                     /locus_tag="J8V14_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBA0921732.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="J8V14_001624"
     CDS             18024..19748
                     /locus_tag="J8V14_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA0921733.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="J8V14_001625"
     CDS             19745..21196
                     /locus_tag="J8V14_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0921734.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6870 a         3608 c         4316 g         6515 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgg
//
LOCUS       DADQRX010000047        21298 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.048, whole genome shotgun sequence.
ACCESSION   DADQRX010000047 DADQRX010000000
VERSION     DADQRX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.048"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..710)
                     /locus_tag="J8V14_001626"
     CDS             complement(99..710)
                     /locus_tag="J8V14_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA0921735.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(833..958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1040..1756)
                     /locus_tag="J8V14_001627"
     CDS             complement(1040..1756)
                     /locus_tag="J8V14_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBA0921736.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1762..2331)
                     /gene="scpB"
                     /locus_tag="J8V14_001628"
     CDS             complement(1762..2331)
                     /gene="scpB"
                     /locus_tag="J8V14_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBA0921737.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2315..3112)
                     /locus_tag="J8V14_001629"
     CDS             complement(2315..3112)
                     /locus_tag="J8V14_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBA0921738.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3082..3453)
                     /locus_tag="J8V14_001630"
     CDS             complement(3082..3453)
                     /locus_tag="J8V14_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA0921739.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3472..4359)
                     /gene="xerD"
                     /locus_tag="J8V14_001631"
     CDS             complement(3472..4359)
                     /gene="xerD"
                     /locus_tag="J8V14_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBA0921740.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4455..4928)
                     /locus_tag="J8V14_001632"
     CDS             complement(4455..4928)
                     /locus_tag="J8V14_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBA0921741.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5033..5896)
                     /locus_tag="J8V14_001633"
     CDS             complement(5033..5896)
                     /locus_tag="J8V14_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA0921742.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6092..8191)
                     /locus_tag="J8V14_001634"
     CDS             complement(6092..8191)
                     /locus_tag="J8V14_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBA0921743.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8411..9520)
                     /gene="rpoD"
                     /locus_tag="J8V14_001635"
     CDS             complement(8411..9520)
                     /gene="rpoD"
                     /locus_tag="J8V14_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBA0921744.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9544..11385)
                     /locus_tag="J8V14_001636"
     CDS             complement(9544..11385)
                     /locus_tag="J8V14_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBA0921745.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11567..13960)
                     /locus_tag="J8V14_001637"
     CDS             complement(11567..13960)
                     /locus_tag="J8V14_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBA0921746.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14067..14927)
                     /locus_tag="J8V14_001638"
     CDS             complement(14067..14927)
                     /locus_tag="J8V14_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBA0921747.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14986..15507)
                     /locus_tag="J8V14_001639"
     CDS             complement(14986..15507)
                     /locus_tag="J8V14_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBA0921748.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            15827..16546
                     /locus_tag="J8V14_001640"
     CDS             15827..16546
                     /locus_tag="J8V14_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0921749.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            16572..19163
                     /gene="mprF"
                     /locus_tag="J8V14_001641"
     CDS             16572..19163
                     /gene="mprF"
                     /locus_tag="J8V14_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBA0921750.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19351..20538)
                     /gene="tuf"
                     /locus_tag="J8V14_001642"
     CDS             complement(19351..20538)
                     /gene="tuf"
                     /locus_tag="J8V14_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA0921751.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20906..21262
                     /locus_tag="J8V14_001643"
     CDS             20906..21262
                     /locus_tag="J8V14_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBA0921752.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5842 a         4468 c         3727 g         7261 t
ORIGIN      
        1 aaatactcaa aacaaacaaa taaaacgttt acattattgc atgtcttgta tttttttcca
       61 aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt atttgactgc tgtatgacgt
      121 tgttcgaact gatgttgttt tcgacttaac catggaagaa gttgcgcatg cagcactaaa
      181 aagacagcac tcacgatgaa tcctttgatc aagttgaaag gaacgatccc aatcagtaca
      241 tagtttgcta aagacatgcc cagcatctgt gttgcgttca gccctaaaaa cattaagtaa
      301 agaggcgtaa taacgaaata atttgcgata ctcataaaaa tagttagagc gatggttcct
      361 gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt tctttacact aaagaaataa
      421 taaatcggga atgtaaagat cgttgttgat aagaaactag cagtgtctcc taccaaatta
      481 tccggagaaa aacctgtagt cagcagatgt aatactgaac gcaagaacgc tgttgcaact
      541 ccggccgctg gaccgaataa gaacatgctg atcaataccg agatatcact aaaatcgatc
      601 ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat actgtaaaac taatgcgatc
      661 gcagcaaaca tcgctactgc aaccattttc tgtacacggg tgttcttcat ttgaaatcct
      721 cctgttagga ctcctcttca gcgaactttt tgacgtttct ctccaaatga aaaaactcta
      781 ttttccaggg aaaatagagt tagagttatt tgatcaagcg tcgtcaaata agctctatct
      841 tctttatcca gactatactg tcggtatcgg aatttcaccg attcagctat ttttttgcaa
      901 aataggtcgt ggactataac caccggtcgg gaatttcacc ctgcccctga agacgaacct
      961 tatataattt ttgtttacat attcattata catatctatt caaaaaacgc aacatctaac
     1021 acttataaaa agtaagtgtt cattttgagt ggttcagcaa actgctgact tcttttttgt
     1081 tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt cagatctccg tatttttcac
     1141 gttttagttt ttgcacagga agaccaactg cttgaagcat atttttcact tgatgattac
     1201 gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt cttaggatcc actgataaaa
     1261 tttggaattt agctggagat gtttttcgtc cttcgatctt tattcctttt gtcaaaggtg
     1321 caagcatcga ttttgtagcg atccctttta cctttgcaac gtaaacttta tccacttcat
     1381 gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc aagatccccg
     1441 aagtatcgta atccagtcga cctacaggat agatacgttc ttttaccatc ggcaaaagat
     1501 ccgtaacaac ttttcttcct ttgtcatctg caacagcaga aataactcct ttaggtttgt
     1561 acaataaata gtaaccgtat tcttcttgat aaattgggac ttcatccact tccacagtgt
     1621 catgtttccc aacttttatc cctaattccc ttacaacttg tccgtttact tttacacgtc
     1681 ctgcaaggat gaattcttct gcttttcgac gagaagtgat gccagcgtgg gcaattactt
     1741 tctgcaatct ctccattaca attattctcc tttattcatt ccatcaaaga atagatctgg
     1801 tgacacgtct tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt cttctaatga
     1861 tttcaaacca aaataatcta agaagtagcc agttgttcca tataatatcg gccgacctgg
     1921 tccttctact ctaccttttt cttcaatcag tttatgggca gctagacgct gtaccgctcc
     1981 tgaaacctga actcctcgaa tctgctcgat ttcagctctt gtgattggct gtttataagc
     2041 gatgattgct aaggtttcga cggcggcttg agataatgtg tttgatgtcc cttgcgcata
     2101 tcgcttaagt aagggagcca gctctttttt agtcgttaat acataatggc tttccgtttc
     2161 aaaaatatgc aaagctgagt gcgagttttg agtgtaagat tcttttaatt ctttcactaa
     2221 acttgtgact tctgcttcgc tgagatttaa cagtccgctt aattcttcat tactgatccc
     2281 ttcctcgcca acaataaaaa gaagggcttc aagttcactt aatttcgtca tgttcggtat
     2341 ctcctactgc tggataaagc ataattgatt catagttttc tttttgttgc gcaatgatct
     2401 gccctttttt catcaattct agcaaagcca taaaagtagt gacaacttca cttttagaag
     2461 gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt tctttttata cgctcttcga
     2521 taaataccat tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt tccacttttt
     2581 gagaaaattt cttacgttct aagattttct gcatggcacg gatcaaatcg atttttttga
     2641 cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc atcgatgttc ataggttctt
     2701 ttgtgtaata aagacttcgt tcctctgctt tttctgtcag tctttcagct gcatatttat
     2761 atttacggta ttctaaaagc tgagtgacca attcttctcg aggatcttct tcccataatt
     2821 cttcatcgtc tgtgacgaat tcttggacag gaagcaatgt cttgcttttg attgccatca
     2881 atgtagcagc catcactaag tattctcctg caacagctag ctcgagtgtt ttcattgtat
     2941 ggatataatt catatattgc tccgtgatcg tagcgatcgg tatatcatat atatcgattt
     3001 ccatttgctg aatcagatga agcagcagat cgagaggacc ctcgaataca tctaatttaa
     3061 tattgatctc cgtcaactca ttcactcttt cttctttttc tttgctagcc attttccaat
     3121 gtaaggctct gtgatttcag ttgcacggat ggagacatcc gggtactttt cctgtaattc
     3181 atctaacatt tgaaagccca tattctcacc gcgataagac ggattcaaag aaatgtcatg
     3241 gatgatcagc tgatcgttcg ttgcccagta aataccaata atcccttgaa tgttggttga
     3301 accttctggc gtaaagaaaa aaagttcata atcgtcttca gattcgtacg tatcaatctc
     3361 cttcaacaaa gaagttttac ctgtcagttt cttatggaaa ctcaatagtc ccatagcaat
     3421 tttacggttt tctttccggt aatgtgttaa catcttctca ccttcctatg actaagccct
     3481 tggaaaatgc tgcttataaa catccgtcat tcttttcttc gtgatatgtg tataaatctg
     3541 agtcgtagag atatccgcgt gacctaacaa ttcctgtact gtccgcaaat cagctccatt
     3601 ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt ctttgtaaat
     3661 cccagcctct cgtactaact gcttcagatt tttccagatt ccttgccgag aaagaccttt
     3721 cccatgatta ttgacaaata catgcatttc agatgcgtcc tttacaagta atggacgtgc
     3781 ttcgtctaag tagcgttcca gccattggat cgcataatca cctaaaggaa tgatccgctc
     3841 tttatctcct tttccaactg tctgtaccag tccaagagaa agatgaagat ctcccaattt
     3901 caatccaatc aattcactga cgcgcatccc agtagcatac atgacttcca agattgcacg
     3961 atctcttatc cctaacgttt ttgtcgtatc aggtgcttca atcagctttt ctacttctgt
     4021 cagagtcaat gtacttggta atttctgtgc tttcttcggc gtatcgatat gttgcatcgg
     4081 atcatgatca gtgatacgct cttgccgtaa aaactgatgg aatcttctga gacttgagat
     4141 catccgagta acagttgctg tcgctttttt ttcttcatgc aacttctcca gaaaacggat
     4201 gactaaaaag cgatcgacat cctgccatga agcaattttc tgctcctgta gaaaatgcaa
     4261 gtactgttcc aaatcacgtt catagctttg tcgtgtattg gcggacaatc cccgttcaat
     4321 attcaagtag tggagataat cgttgatttg ctctttcata ataatccccc tcatgcctct
     4381 ctatcatatc agataccgct tcattctgat agtctttttc cataatctga gcaaagaaaa
     4441 agccgtctag acactcagac ggactggttt cttttttctt tttcatagca ttcctgacag
     4501 attccatgga acgttagccg atgatctttt actaaaaaat gataacgttc ttctactact
     4561 tgctccactt caagtaaaag atcctcttct acttcctcga tcgtgccgca gttcaagcat
     4621 agtaaatgat gatggaaatg tttggcgcct tcttttctca aatcataacg ggctaatcca
     4681 tcattaaaac ttactttatc gacaattttc aattctgtca agatttcaag ggttcgataa
     4741 acagtagcaa gtccaatttc cggactcttt tttttcacta aaaagaagat ttcttctgca
     4801 gataaatgct ctttctcatt ttccaaaagc acaagcagag tagcttctct ttgcggtgtc
     4861 agcttaaacc ccgcttcatg tagctgctgt tttgtttttt tcatcgccga aatagcattc
     4921 atgaccattt actcaacccc aacaatttat aataattaca aataaaaaaa gatataacac
     4981 tatgttaatt aaaattatta taagctagtt aagaaaaaca tgcaagggat tctcaattag
     5041 attttttgac tagttttgtc acaccgtttt cttgtgtaat caatccttgt ttcaaaagat
     5101 tacctaatgc gcgcttaaat gctcctttgc tgataccaaa agctttcatg atttcttctg
     5161 gatccgattt atctgtaaag ttgatttgct gatctgcaga acgttctaaa atggttaaaa
     5221 tcatttgtgc atcatctgat atagcttcat atgctcttgg cttcaatgaa agattcagta
     5281 caccgtctgg tcggacacca atcactcgac cttttatctg ctcgcctaga cgaggttcat
     5341 tatatcgttc agaaggatgg ataaacccaa gatagaaatc ttccgttaaa acgtacgtac
     5401 ctgttaattt caagcggtat gctgttccta tcacatcttt gtttttcatt tcttctgttc
     5461 catatttgct taaagatttg aatatttttt catctgctaa actgccccaa atacggtctt
     5521 ttgcatcaac tctaagggaa atcatcaagc gatccccttt tttaggccat agctctgtca
     5581 ttgtcggcaa ttcatcaaga gatacgacaa agtctttatc tggaagaccg atatcgacaa
     5641 acgcacctaa atcttttcgt gtagcagtaa cgattccaaa agcgtaatga ccgatacggc
     5701 ttttaggaat ttgtgtagtg atccgatttt catgtttttg attttggtaa ccgaatcctt
     5761 caactgcttc accgattttg tgttcacctt ctgatttgct taagccaaag gtttgtccgt
     5821 ttttttggac aaagtagttt ttttcatttt catcaataat aatacctgta aatatagttg
     5881 ctaataaatc gttcattttt ttctccctct atagtgaaag gttttcttaa tacatttctt
     5941 tgctatcata acataatgtt atctacttga atagctgagc cttggtttgg aaaggtgaaa
     6001 aaaggagtat gacaacagtt gtgtcatact cccctttttc gaataaacgg tgttaaacgg
     6061 caggctcaca gcctctttca tttgcctaaa attaattgct cccatttcct ccgaaccatc
     6121 cagttttaca gttgatggtt acgatgtaaa atggcaaagc actttctcca atgcctttca
     6181 tcaatgcaga ttttgttgaa gtggcatata gattataata gccttcccca ttttcgattc
     6241 tggctttttc taatctgtaa cctccttcaa cgatatatcc acgttggata catgtagcaa
     6301 tcactttgct ttggacacct ggtgcccacg tccatgctgg attagatgca tcggcaatat
     6361 cacttgctct ggcagatttg attggctgat tcgctgagtt tgaagaggaa ggggtcgagg
     6421 atgccgcttc ttgctgtgct gcttgtgcga ccgcagcttg ttgagcggct tgagctgcag
     6481 ctgctgcttg agcggcttgt tttgcttgtt cttcagctgc ttttttcgct tcttctgctt
     6541 ttttcttttc ctcttcttct tttttctctt tatcagtgag catcttatct acttgtttca
     6601 attgttcatt caactgagca gatagatctg tatttttcac tttttctact gcttcttttg
     6661 ccgtcttgta ttggtcgcgt gttgctgaat ccactacttt tccgtcttta aagactatag
     6721 ctgttttttc tttagctgtt tgcaattcag catattgatt tttagcttct gtttgtgctt
     6781 gttgaatcaa ttttccaaaa tctgtgttat ccgtttcgat tgcttggatc tctttatctg
     6841 ctttcaactt gatgtcatct gctagatgat catcgacgat ggcaggtgct atgaaccact
     6901 cattcacttt ttggatatct cggatcttag attggatatc ttggtaagtt gtttctaact
     6961 ctgaatatcc tttttcattc ttaagactgg ataaatcatt ttttagtttt gataaatctt
     7021 gatgaatcat atctgcacga ataaattgat ggtgatcatc tgtataaaag gcagcaagtt
     7081 tctcatgaag ttcttccatt tgttgctgtc tttttgcttc tgctgctact tcggcttcta
     7141 ctttttgatg atggtcataa taagcccagc ttcctccacc gagtagaagt actgctgcta
     7201 aaacgagata aggtactttt tttgattttt tcttttgcgc tttatctgga tctgtttttt
     7261 ctaagtttga ttcttgatgt gttttttcag tatctggcac gattgttatt gatttcgctt
     7321 ctgcttcaaa cagcttaggc tggttctctt ctttttctat ttttgcttca agatcagtac
     7381 cctctttgct tacagattga ttttcagcat cggattcact atttatttca tcgggctctg
     7441 ttgacttttt gtctgtgttt tcgagtattt cttttttctc tgcttccggc agtttattcg
     7501 ttttttcctt ttctaattgt ttttcagtaa atacttcagt tttttctgtg atatccgttt
     7561 ttacgtcttt tatttttact gtttctttac ctgtggcgtt ttctctatca gagtcttttt
     7621 cagtttcttg ttccttttct ggctctactt tgttttcctg tgcaattttt tcactgatca
     7681 ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct ttgatataag
     7741 cggatagtat cgaattttct tcctcattat cgacgatttc tttcagttca gctactcttc
     7801 ttttttgttt tgattcaagc gtttcttctt gattctttcc tgattcttcc gaagtctcat
     7861 gaacagttgc tttcttctgt tcatttttct ctatattagt tacttgatcg tgagcaggtg
     7921 gttcttcgtc ttctggttct gctttttcga ataattccat tacagattct agaggtaaat
     7981 ctttcaattc cgaccattcg attggatcgt ttaattctgt atcaggaaag actgcatctt
     8041 cagttgattt atcctgcata gctgcatctg tatttgtttc ttcaattgat tcttttattt
     8101 gctgatcttc tgcagttatt tgatgaccgc agtctggaca gatagtattc tgctccattt
     8161 ttttcgatcc gcaatttgga cattttttca tcacttagcg atcggctcct ttactaggtt
     8221 tacgctacca tttaatctat catattctat acgtcacgcc aatagcttat ttttaggatt
     8281 tacttaaaaa taatgaatga atttccttcc tatagaaaaa aaagcttgag actgcgtagt
     8341 ctcaagcttt ttttctttta aaataaacca aacagtttta ctgatttctc ttctggtcaa
     8401 tagtccacgc ttattctaag aaatctttta attgttttga acgagaagga tggcgcaatt
     8461 ttcttaatgc tttagcctcg atctgacgaa tacgttcccg agtaacacca aatactttcc
     8521 ctacttcttc caatgttctc gtccggccat catctaaacc aaaacgtaag cgaaggacat
     8581 tctcttcccg atcagtcaat gtatctagta catcttccaa ttgttctttt agtaactcat
     8641 aagctgcgtg ttctgctgga cttgttgctt cctggtcttc tatgaaatca cctaagtgag
     8701 agtcatcttc ttcaccgatt ggcgtttcaa gggaaacagg ttcttgcgcg attttcaaga
     8761 tttctcggac tttttctgtt ggaagatcca tttccgcacc gatttcttct ggcgtaggtt
     8821 ctcttccaag gtcttgcagc aattgacgtt ggatacgtat caatttatta attgtttcaa
     8881 ccatatgaac tggaatacgg atcgtacgtg cttggtcagc gattgctcga gtgatggctt
     8941 gacgaatcca ccatgtagca taggttgaaa acttaaatcc tttgcggtag tcaaattttt
     9001 caactgcctt catcagtccc atgtttcctt cttggatcaa gtcaaggaac tgcatgcctc
     9061 gtcctacata acgtttagcg atacttacta ctaaacgaag gtttgcttct gccagacgct
     9121 gtttcgcttc ttggtcgcct tcttcgattt tcaatgctaa cgctacttct tcttccgcag
     9181 ttaaaaggga gacacggcca atttctttca gatacatacg aaccgggtca ttgattttta
     9241 caccggttgg cgcagaaaga tcttctgttt ttgcttgttc agcttttttc tcagcactct
     9301 tcaagctgtg aattgatgga tcaccatttt catccaccac actgatacct gcgtcttcaa
     9361 tcttttggat caatttgtcc atttcatctg cattcaaagt gaatggtgta gcaagctgat
     9421 tggataaatc atcatataca acttgtcgtt tgggtttgtt ttctttgatg aaagctgcta
     9481 ctgctgcttc atacgttttt ttatctgttc catttgccat aaaagaaggc cccccttgag
     9541 tagctatttg gcttgtttta actgtttcgt taaattgatg atttcaatcg tcaattcaag
     9601 ctcaagctgt ttgtttccag ttcgtctggc ttcttgctgc atttgttttt ttaatgttat
     9661 ttcatcagct aagccagatt tgctgatcac atctagcaag tcttggattt ctcgtggcga
     9721 actttcttct gccaccggta aatattcgat ttccaccacc aaccgcctta aattttcatc
     9781 ttgcaaaaag tctaaaaact ctgcaaggtt gaattcataa tgttgtgaga catatgcatc
     9841 gaataaaaaa taaacttctg catatgcatc atgaataaac tgaaattctc gttcttttaa
     9901 aatattacgg acagaaactt ctttaaatgc acgatataat agaatctgct ctgccttttc
     9961 tacttgcgtc aaaggtctct ttttccgtgc agtttcttgt ctcgtaacct gagtgtgttg
    10021 actttcctga ggttcttgac gattttgctg atttgtcctt cgaagatcgc gcagttgttt
    10081 ttgtatcgtc tctctgccga tctgaaactc tgttgacagt tgagatagat acatatcttg
    10141 ttcgattggt gaaacaactc ggctaagttc aactagtaat tcttcgaggt attcgatctt
    10201 gtctttttca ttatctaaat ttttcccttg ttttaaataa cgtgatttaa aagtaaagac
    10261 tgtttcgcgc ccatgagcca ctaattcttg gaaagatgct tctccatact tacggagata
    10321 ttcatcagga tcaagctttt ctggaacgac tacgacagaa agtgctaatc gactgttttg
    10381 ccgaagcaat tcaatccctc gattggttgc ttcaaaacct gcgttgtctc cgtcatagca
    10441 aaagaccaat tctttcgtca gcttttccat ttgttggatc tgttgagctg ttagacttgt
    10501 ccccatagaa gctaatccag ttttgatacc agcctgccat gcagcaatca catccataaa
    10561 cccttcgaac aacaaaattt ctccgttttt ccggatatcg cttcttgctt tatcaagatt
    10621 aaataaaact tgtcttttat tgaacagttc tgtttctgga ctattcaagt atttgggctg
    10681 atctttttcc tgcccttttt caggtgcaag ccagcgtcct gaaaaaccga tcgtctggcc
    10741 ttgagaatta cgaagtggaa acatcactct ttgataaaaa cgatcagaca tacggccatc
    10801 atcatgcgtg acaaacaatc cagagcgttc taacaattca tttgaaattg attcattctt
    10861 gaatacttgc tgaagaaaag aacgctcatt aggtgcaaaa cctatgttga attcttcaat
    10921 taattctaaa gacaacccac gactttgcaa atattctaaa gctggctgtc cagcttttgt
    10981 atgcaatagc atgtgatgat agatttccgc tgctttttca tgagcagcaa tcagtttccc
    11041 cgtagcagat tcaggatttc ggacagctat agtacgctga cgccattgat cttctaacgg
    11101 tatctgttcg aagtctgcca cttttaccac tgcttctgga aaacttagac cttctagttc
    11161 ttgaagaaaa gtaaacacgt ttccgccttt accacaacca aagcaatgaa aaatctgctt
    11221 gtcttctgca acagaaaaag acggtgtacg ttcttcatga aaaggacaca gccccaagta
    11281 atttttacca gactttttca actggacata ttgtccaatc acctcaacga tattcgtttg
    11341 actgcgtact gtttcgataa cctcttgagg aatccgttga gccatttttt caccccctgc
    11401 tctattgata tcttttgggt ttaacagaag atatttcagg agaataaccg gtaaaaaatc
    11461 gcactaactg aaatcagtgc gattttaaag gacacaatta tacatgtttg attgtatcat
    11521 atttcattgc tttttcaagt ataacacatt tctatctcat taagcatcaa tttgctactc
    11581 ttttcagtaa ctgattttga atatcagcct tccagtacaa tactcgaaga acaatattta
    11641 cgagcatgaa tacaaagaag tatcctacgc tctctcccaa ccctaagttt acttccaaaa
    11701 ttccatggaa cagcattgca gctacatata tccctacgat cgtcgttact gctaggaaga
    11761 tgcgcaatgg gttgaaggga taacaagctt ttacaactgc catgcaactg atcccaatca
    11821 gcaagtaata catcaaagta gtagtttcag catttcccca tgcgaatgtt tttccaagta
    11881 gatacaccgc aatgatattc actactacaa gcaaggcatt tggtaaagca ttaataagtg
    11941 ctgtaggcaa ataacggtaa gtgatttttc ttttatcttg ttcaaaagat aaaaagaagg
    12001 aaggataccc ttcaatcgct aaatcaatca gcgtgatttg gatagggata aacggaaatc
    12061 caattgctgt aacggcacat ataatcgata aaataaatga ataaatcgtt ttgataaaaa
    12121 agatgcctga gactttcgtc acattgttga ccactcggcg tccttcaaac aggacttctg
    12181 gcaatgtagt aaaatcagag tccaacaata ccagattcgc aatctgacgt gttgctccat
    12241 caccttctgc catagcaatg ctgcaatctg cttctcttaa cgccaaaaca tcattcacac
    12301 cgtcgcctgt cattgcaacc gttctgccgc tttctttcaa ttcatttacc agcaatttct
    12361 tttgctgcgg actcactcgt ccaaaaaccg tatagcgatg agcagcttct cgcacttcca
    12421 tttcttcagt aatttgagaa agatcgacat acgcatcata attcggtaat cctgcacgtc
    12481 tagcgatatt ggagacagta aaaggattgt ctccagaaat cactttcaga tccacacctt
    12541 cctcatgaag ataagctagt gtttcttgag cgttttttcg gattggatca tcgatttcaa
    12601 acagagcaac cggctcaaca aatgctggtt tttcttcgtt caattgctga tttggagcga
    12661 tccccaacat aagtacgcga tagccattcg cttgggctgt ttttagactg tccggaagcc
    12721 gatcttcttc caccaaacgt tcaggcgctc ctacgtacac gactcccagt tctggaaatt
    12781 ccatcgcgcc ccatttgcga tcagaagaaa atgcgacttt gtttgtcaca tcataccgat
    12841 tagaaagctc aaaataatct ctcatagccc gcatagtgat gttgttatca ctgctttctg
    12901 ccagatacgt ccccataatt tctgggatgg ctttctcgta cagcggatgt agtgcttcta
    12961 cccgctggat actcattttc ccttcagtaa tggtccctgt tttatccaag cataatgtat
    13021 cgacatgggc cagcgtttca attgaataca tgtcttgaac aaggattcgt ttttttgcca
    13081 gtttggttac ccctgtcgtt aatgcaatac ttattagcaa taccaaaccc tttggaagca
    13141 tgcctaacaa agctgctgca gatgcaataa cagaaatttt gatagaagca tcgcggatga
    13201 acagtgcttc caaaaatagt atgatcccca aaggaataat cacgaaacta gtgaatttgg
    13261 atactttgcg aatcgaatct acaagttcag aacgaatagc tttgtgagtc tttgcctcgc
    13321 tagtcaagcg gacagcataa ttatctgcac ctactcggat aacttcagcc atgacagaac
    13381 cgcttgtcaa aaagcttcca gaaagcaatt cagctccagt ttctttgaca atcaaatcag
    13441 attcgcctgt caaaagagct tcatttgctt ctgcccgtcc agatataacc tgtaaatcgg
    13501 ctggtatctg ttctcctgct ccgattttta ccaaatctcc taataccagc tctttagtag
    13561 aaatcgattg ttctttaccg tcccggatca ctagtacttc tgattgggaa acaatcgata
    13621 atttccgtac aagatttctt gcatgtattt cttgataaat tccaataacg atattgacga
    13681 caataattgc taaatacgcc atgttggaat acgcacctac aaataacaga caaatcccaa
    13741 taccaaagtt cagaaaattg aataacgtca atatattatc gctgaatatt tgttttgtac
    13801 tttttgatgc atcttcctgg tagtcatttt gctggccaga agccattttc tgaactacct
    13861 cttgctgtgt aagtccattc attttctttt tctccatttt ccgccaactt tcaaaaataa
    13921 tcacgtgttt aagtctagcg tgttccaaga ttaaaaacaa cttgttttta taaaatttat
    13981 agaaaaacac acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg aatcacccgt
    14041 gtgctgtttt tattatcaat ctatctttac ttatattgtt cctccaaacg gctcatccac
    14101 caacctagtg ctgtaccggc tataaaaaga acaggacaag ctagtacccc agcaactccc
    14161 aatcccgcgt aatcggtagg aatgatcata atcgttgaag caaaaactac acctaaaatg
    14221 aaatggaata gcttgctata tgcctttgag aaaatgtaat ccatgatttt tgacaatcca
    14281 agaacacaaa gaatcccacc aattccgatc ggcacaatca cttcaagtcg tacttcttta
    14341 aatccgtcag ccatcgcttt atacaatccc atatacacca aaaaattaga agggctcaat
    14401 cctgggacga tcatacccaa accaatcaat ccgccggcaa tcagccatgt tccaaaattc
    14461 tgatcgactt gcccgtcaaa taattgagcg ccgtataata aaaacaaata agccagaaca
    14521 aaagtaacaa ccatgatgat cacatcagaa gtatctcttc cttttttccc tgcttctttc
    14581 cataacgcag gaacagttcc agcgatacat ccgacaaaaa accataatat cgttgtttca
    14641 tacgcaccta ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt cactccgcct
    14701 agaccaacag gcagaaaaaa caaaacattt tctttgaaat tttttgtaag gtgggctaaa
    14761 aaagaaatca gccgctcata catgccaaag acagctgcta atgcaccacc gctgactcct
    14821 ggcaagataa atcctgaacc gataaacatt cccttcacaa accgtaacat ccagtctttg
    14881 gttgtgtacc ccacttcttg attttccatt ttttgccttt tttccactat ttgcgtactc
    14941 cttctttcat ttaattttat tcatgattga acatttcctg ctattttact taaagacttt
    15001 ttcataggtc tctattattt tttcattcat tttgacaata tcaggataaa acatgttacc
    15061 tccattatga tagagatagc cgccattgta aagcaaggac tggatccgcc aatatcggta
    15121 ttgtttccct tcctcatttc ctaaaagtgg gctcagcaca tctttagaat acgcttctgc
    15181 taattctact gtgttttctc cgccatgatt ttttacatag gcaatataat ctttcccaaa
    15241 attatatgct tggacagctg tccataaatc gcatccttgt ttctttgctt ctactatcat
    15301 ttccgccaaa tatttgacac cttgatcaat actgtcagct ggattatcaa tgatctcagc
    15361 ttctccatgc agactttccg agctctgcat gagatctgtc ccttttcctt ttgattcggt
    15421 caatattatc gccttgatca aattctcgta gttttccata tgataatttt ttgcagctgt
    15481 tgttatttct atttgatagc tttccactcg ttttaccgaa cgataagttc ttcctcataa
    15541 aaacaacacc gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac ggataataga
    15601 taatcgtttt cgcttcattt catcacctag ttattttgac atcttttttt atcttactat
    15661 ttctttatct tcttggctat ttcttccttg atttctgtgt acgccttaag aaaaaatgaa
    15721 ttttcctgta ttttttgaca tcttacgaaa atgatcgttg tttttcagta aaaacaattg
    15781 cccaatgact gatataatcg ttatacttta cacagtaggt gattatgtga aaaaagaaaa
    15841 gaaatatgca aaaatgccag atgggagcga aatttactat gaaaagagcg gtcaaggctt
    15901 ccctctcttt ttactgcatg gcaatgacgg cagcggccgc tttttttcag aacaggtccc
    15961 tgtactcgaa cgctactata ccgtgtatct agtagatagc cgaggacatg gtcgttcgac
    16021 aaacgaagca tctatgttga attttcagtt aatggccgag gatctgaata cgatcatgtt
    16081 gctggaaaaa atcgatcagg cagattttct tggatttagt gatggagcaa atctagcact
    16141 ggtatttgcc agcagcttcc ctaaaaaagt tcatcgattg attttaaatt ctggaaacac
    16201 gcttgttaaa ggcgttcgtt tttctgccag agtcatcagt aatattcatt atgcatgggt
    16261 atggttactc tctttatttc gcccaagtct tcgcaaaaat cttttagtta tcaaactttt
    16321 gctgcatgac atcgggctta cagaaaatga tttaaaaaaa atcaactccc ccactttgat
    16381 catcgtggga aaaaaagatg taatcaagct aaaacattct ttgtatatag ctaaaactat
    16441 accaaaagct tcctttgtgc ttgtgaaaga acaaggacat gaattagcta gaaaagatcc
    16501 tgaacgcttc aatcgagaag ttcttcaatt tctttctgaa acataactat taggaataga
    16561 aggtgattta tttgttaaaa aataccatac aatggctgaa aaaccaccta ggactgttca
    16621 aaacaatctt tttgatttct gtcattgtca ttattgtcgg tgaattgatg tccatcggta
    16681 aaactctatc tatccagcaa ttaggagaaa cgtttgccac tatacctgta tggaaaagcg
    16741 gactaatgct attgattggt cttatctctg tgttacctat ggttggttat gatattattt
    16801 tgaatcggat gcttgaacaa aagcagaatc ctcgttacct gtttgagaca agctggctga
    16861 tcaatacgat caacaatatt gccggtttcg gcgggtttgt tagtgtgggg cttcgctctg
    16921 aactatatgg acaaaagaaa gagagcagaa acgtgatcca agcattatcg aaagttttct
    16981 tgtttttgat ggctggactt tctatttaca gtctgctttc ttttttactt gtagtcttca
    17041 cgccagttgc tccttttttg aagcaatatt ggatatggtt gatcggcggc ggtctatatt
    17101 ttcctgtcgt ctttttattt actactttca aaaagaaagg attccttggt ggtacctcac
    17161 ttaaaactca aggacagcta ttgctagttt cattgttgga atggaccggt gtcctgttta
    17221 gtttcctttc tgttggttat ttaatggaaa tccggattga tctgtggcaa acaattccat
    17281 tattcattgc tgcttcagtg ataggaatcg tgtctatgat tccaggagaa attgggagtt
    17341 tcgatgtaat gatgattatc gggttgtccg ctattggtgt gccaagagaa atagtcgttg
    17401 tgtggatctt gctctatcgc ctgttctact acatcattcc gtttttgatt ggaatcgtct
    17461 ttttcttcaa aaatatcggt tccacttttg atcagcgtta ttctggtatc cctaaacaat
    17521 tagcaacaga aattgcgcat aaaatcgtgg ttgtcttatt atacttttct ggaatcatgc
    17581 tagtactttc tgccactatc ccgcaagctt ttacagagtt tcgctggctt catagtttga
    17641 acccattgaa atttcatttt attatccaat ttcctagtat actattggga tttcttttaa
    17701 tcattatggg cagagggatc gcagcacgtg tgaaacgagc atatttgcct acaatcttct
    17761 tgattgttct agctttactt tatgttttat taagtgattt tagtttcaca ccggtggttt
    17821 tcttgtctat tctgcttctg atcattcttg cttctaaaaa cgaattattt cgggaacaat
    17881 tgatctattc atgggagtgg cgtacgatcg atggaatcat tattggtgca ctgtccttgc
    17941 tttacatcgt tattggggta tacaatctgc cggatttccc tcatcggcgt caccatttta
    18001 tttccttttt cttatttcct tctgaaaaga tttggttttc gggattgttg gccatcatcg
    18061 ctgttagttt catgatcgtt ctatttgttc atttccttca aggagaaaag aaacagattg
    18121 gcgaagcctt taatgaagaa aaggccctga agatccttac aacttatgga ggaaactccg
    18181 atagtcaatt gatcttctta aaagataagc ggatgtttgc ctatgaaaaa gacggtgaac
    18241 caactgttct tctccaattc gcttgcttca ataataaatg tattgtaatg ggtgatcctt
    18301 ctggtaagaa agaagatttt ccagaagcaa tcgaagcttt tatcgaagaa acagatcgac
    18361 tatgctattt accagttttc tacgaaacaa gcgaagagat tgttatgatt ttgcatgaat
    18421 tcggctacga tttcattaag atgggggaag aagcttatgt agacctgaat agttttacaa
    18481 cttcagggaa aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa cgagaaggat
    18541 ttacatttga tgtcttacaa ccaccatttt ctactgagca aatgagtact ttcaaaaata
    18601 tctctgataa ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc tttttctctg
    18661 aagattatct acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa gaaattgtcg
    18721 cttttgcaac gatcatgcct acttacacga acaataaagt aggaacgatt gatctgatgc
    18781 gatatgattc taaaaaagca ccttctggca gtatggattt cttgttcttg catttgtttg
    18841 cctatatgaa agaagaacat atccaatggt tcaatttagg gatggcccct ctttcaaatg
    18901 tcggaacttc cagaaagagt tttttacagg aaaggattgc atacttggtt tacgagtttg
    18961 gttctcattt ttattctttc catggattaa gagaatataa aagcaagtac gctacaaact
    19021 gggtctcacg ttatactcta tattctagag atagttggat tgcatatgtt atgattgctt
    19081 tgctgattat tgataatgcg ccagttgagc agacacaagg tagaataagt ggtttgaaaa
    19141 aatggatacg aagaaagtat taaaaactgc ataccgatag atgaacactc tcatgatgaa
    19201 taaaagagac tgaagtaaaa cgaaagttca tgcttctaca atctcttctt atacaattga
    19261 agaacaaaga gcggtccaaa cagaaatcct gtttggaccg ctcttttatt ttaaacattt
    19321 ttcattacca aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa cgatacctga
    19381 acctactgtc cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa cagcgattgg
    19441 atggatcaag tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt ctgtatcttc
    19501 tggcaatgtg attgttcctg taacatcagt tgtgcggaag tagaactgtg gacgatagtt
    19561 gttgaagaat ggagtatgac gtccaccttc ttcttttgtc aacacgtaca cttctgcttt
    19621 gaattttgta tgtggagtaa tggaacctgg tttagcaagt acttgtccac gttcgatatc
    19681 gtcacgttgg atcccccgta acaacacgcc tacgttatcc ccagcttccc cgtaatctaa
    19741 cgttttacgg aacatttcta cacctgttac aactgctttt tgtgtttcag gtttgatccc
    19801 tacgatctct acctcatcac cgacacgaac agcaccacga tcaatacgac ctgaggcaac
    19861 agttcctcga cctgtaattg agaagacgtc ttccactggc aatagtaatg gtttatctgt
    19921 atcacgttct ggtgttggga tatattcatc tactgtatcc atcaattcca taatagcagc
    19981 ttcagcatct ggatcgcctt gcaatgcttt taatgcggaa cctttgatca caggagtgtc
    20041 atcgcctggg aaaccatatt cgctcaataa ctcgcgaact tccatttcta ccaaatcgat
    20101 caattcttca tcatcgacta aatcaacttt gttcaagaaa acaatcaggt attttacacc
    20161 aacttggcgt gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt cagttgcaga
    20221 aacaactaag attgcaccat ccatttgtgc tgcccctgta atcatgtttt tcacatagtc
    20281 cgcgtgtcct ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat attctacgtg
    20341 tgcggtattg atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa tactagcata
    20401 gtcttgagga tttgctaggc cttttttgcc taatactgta gtgatcgcag cagttaaagt
    20461 agttttccca tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt tacttctgtc
    20521 ataatgttct tttgccataa tttacaaacc tcctaaataa tatagatcac tgaaaaattt
    20581 cagctctcac ctttttatta tagtcttttt ttgactaaat caaaaagaat acgtccgact
    20641 tactttttgt aagattcgaa ctaagcttat tatacgtcaa tattggtcaa agtcaactaa
    20701 gagcactcgt ctgtcttata cgcgaaaaaa gtacctttta taaataaaaa aatgtgtttt
    20761 ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa caaaatgagg
    20821 aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt ctatttcgtt
    20881 aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg tcgttttagg
    20941 catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg aagcttatta
    21001 tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg gagagaaatt
    21061 cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa aacaggtgac
    21121 gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac tcaaagtcaa
    21181 tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc ctaaaaatgc
    21241 ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa aaagaggc
//
LOCUS       DADQRX010000048        20360 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.049, whole genome shotgun sequence.
ACCESSION   DADQRX010000048 DADQRX010000000
VERSION     DADQRX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.049"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            291..1430
                     /locus_tag="J8V14_001644"
     CDS             291..1430
                     /locus_tag="J8V14_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBA0921753.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="J8V14_001645"
     CDS             complement(1497..2072)
                     /locus_tag="J8V14_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA0921754.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="J8V14_001646"
     CDS             complement(2077..3051)
                     /locus_tag="J8V14_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBA0921755.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="J8V14_001647"
     CDS             3334..4302
                     /locus_tag="J8V14_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0921756.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="J8V14_001648"
     CDS             4415..4693
                     /locus_tag="J8V14_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA0921757.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="J8V14_001649"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="J8V14_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBA0921758.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="J8V14_001650"
     CDS             5159..5905
                     /locus_tag="J8V14_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBA0921759.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="J8V14_001651"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="J8V14_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBA0921760.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="J8V14_001652"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="J8V14_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBA0921761.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="J8V14_001653"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="J8V14_001653"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBA0921762.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="J8V14_001654"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="J8V14_001654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBA0921763.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="J8V14_001655"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="J8V14_001655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBA0921764.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="J8V14_001656"
     CDS             10642..11184
                     /locus_tag="J8V14_001656"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBA0921765.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRMQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="J8V14_001657"
     CDS             11207..12763
                     /locus_tag="J8V14_001657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBA0921766.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="J8V14_001658"
     CDS             12777..13679
                     /locus_tag="J8V14_001658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBA0921767.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="J8V14_001659"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="J8V14_001659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBA0921768.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="J8V14_001660"
     CDS             15122..15544
                     /locus_tag="J8V14_001660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBA0921769.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="J8V14_001661"
     CDS             16013..16243
                     /locus_tag="J8V14_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBA0921770.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="J8V14_001662"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="J8V14_001662"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBA0921771.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="J8V14_001663"
     CDS             17654..17830
                     /locus_tag="J8V14_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBA0921772.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="J8V14_001664"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="J8V14_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBA0921773.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="J8V14_001665"
     CDS             18325..18708
                     /locus_tag="J8V14_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0921774.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="J8V14_001666"
     CDS             18877..19761
                     /locus_tag="J8V14_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0921775.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19878..20111
                     /locus_tag="J8V14_001667"
     CDS             19878..20111
                     /locus_tag="J8V14_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBA0921776.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="J8V14_001668"
     tRNA            20199..20279
                     /locus_tag="J8V14_001668"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="J8V14_001669"
     tRNA            20286..20357
                     /locus_tag="J8V14_001669"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3565 c         4425 g         5611 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcatgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DADQRX010000049        20306 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.050, whole genome shotgun sequence.
ACCESSION   DADQRX010000049 DADQRX010000000
VERSION     DADQRX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.050"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..3376
                     /locus_tag="J8V14_001670"
     CDS             <1..3376
                     /locus_tag="J8V14_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA0921777.1"
                     /translation="KKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAEVISDQ
                     SGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITLEISSN
                     TKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIEEESSK
                     ENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVLDHQGN
                     KNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDVYLNAR
                     GNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINMGYVGY
                     SSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPNGHKKV
                     IVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQNNLSR
                     RIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSSDEKGD
                     LYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVGTSPTT
                     VTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTTFQPNI
                     DTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAAAWKNG
                     YIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIKELPVP
                     GYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLTDHGDG
                     TYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVTTSDDT
                     IQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQTTNEKG
                     LASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNVWTLTH
                     QNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLKPGTYT
                     LTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITNQAKVP
                     LPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3379..4800
                     /locus_tag="J8V14_001671"
     CDS             3379..4800
                     /locus_tag="J8V14_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBA0921778.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4797..6674
                     /locus_tag="J8V14_001672"
     CDS             4797..6674
                     /locus_tag="J8V14_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBA0921779.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6766..7608
                     /locus_tag="J8V14_001673"
     CDS             6766..7608
                     /locus_tag="J8V14_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBA0921780.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7760..8635)
                     /locus_tag="J8V14_001674"
     CDS             complement(7760..8635)
                     /locus_tag="J8V14_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA0921781.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8842..9872
                     /locus_tag="J8V14_001675"
                     /pseudo
     CDS             8842..9872
                     /locus_tag="J8V14_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            9961..10266
                     /locus_tag="J8V14_001676"
     CDS             9961..10266
                     /locus_tag="J8V14_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBA0921782.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10445..12460
                     /locus_tag="J8V14_001677"
     CDS             10445..12460
                     /locus_tag="J8V14_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBA0921783.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12462..12746
                     /locus_tag="J8V14_001678"
     CDS             12462..12746
                     /locus_tag="J8V14_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0921784.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12772..14145
                     /locus_tag="J8V14_001679"
     CDS             12772..14145
                     /locus_tag="J8V14_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA0921785.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14228..16243)
                     /locus_tag="J8V14_001680"
     CDS             complement(14228..16243)
                     /locus_tag="J8V14_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBA0921786.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16265..17479)
                     /locus_tag="J8V14_001681"
     CDS             complement(16265..17479)
                     /locus_tag="J8V14_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0921787.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17699..18643
                     /locus_tag="J8V14_001682"
     CDS             17699..18643
                     /locus_tag="J8V14_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921788.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18939..19883
                     /locus_tag="J8V14_001683"
     CDS             18939..19883
                     /locus_tag="J8V14_001683"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBA0921789.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6930 a         3720 c         3867 g         5789 t
ORIGIN      
        1 gaagaaactg aaagttattt tcatgctgat aatattgagt ttatcaaact ttgtgccatt
       61 atctgcaata gcagacacta cagatgatcc aacagtttta gaaacaattt cagctgaagt
      121 catttcggat cagtctggaa aaaaagcact gaacatcaag ctaaatgcga ataacaccag
      181 tgctgaaaag atagaaaaag aaattggtct agtcgaaaat tacttaagtg atgtggaaag
      241 aaaagaagga gatggctatg cttatcaggt aaatagcggg aaaattacgt tggaaatctc
      301 atcaaacact aaacaaacta tcgatctgag ttttccaatc gatccagcac tttaccacag
      361 ccaggcaaac aagctgatcg tcgataataa agaatatgac attattgatg agacagaaaa
      421 taagaaagat acagatgtgt cagtaccaaa gccagacgaa atagaagaag aatcatcaaa
      481 agaaaacgaa aattctgtca gcccatttac attgcctaca ttatccttgc cagctgtgag
      541 tgtgccatct aatcaaacga ttcctacaga atatacaaca gatgatcagg gcacttatcc
      601 taaagccagt tggcaaccta caggaaatac aaatgttctt gatcatcaag gcaataaaaa
      661 cggaacaaat caatgggatg gtataaattc ttggaatgga gatcctaatg atcggaccca
      721 ttcgtatatc gaatatggag gaaccggtaa tcaagcagac tatgcgatac gaaagtatgc
      781 aaaggaaaca agtacacccg gattgtttga tgtttatttg aatgctcgtg gaaatgtaca
      841 aaaagatatc acgcctcttg atctcgtatt ggtcgtagac tggtcaggaa gtatgaacga
      901 caataatcgg atcggtgaag taaagattgg tgtcgatcgt tttgtcgata ctttagcaga
      961 tagcggtatc acagacaaaa tcaatatggg atatgtcggc tactcaagcg aaggatatag
     1021 ctacagtaac ggtgcagtac agatgggttc atttgattca gtgaaaaatc aagtaaaatc
     1081 cattacacct tcacggacaa atggtggtac ttttacacaa aaagcactaa gagatgcagg
     1141 aagcatgcta tccgttccaa atggacataa aaaagtgatc gttttgctga cggatggtgt
     1201 accaacattt tcctataaag tacagcgggt acacgcacaa tcaagcagca attattacgg
     1261 aactcagttt tctaatacgc aagatcggcc gggaaatact tctctaatct caagaatcta
     1321 tgatgcacct gaccaaaaca atctatccag aagaatcgac agtacgttta tcgcaaccat
     1381 cggagaagcg atggcactca aagaacgagg aatcgaaata catggtcttg gcatccaact
     1441 tcaaagcgat ccggcagctg gtctctcaaa agcagaagta gagtctcgta tgcgacaaat
     1501 ggtttcatca gatgaaaaag gcgatcttta ctatgaatca gctgatcatg caacagatat
     1561 ctctgaatac ctagccaaaa aagctgtaca gatctcagca actgtaagca atggacaaat
     1621 aaatgatcca atcgcagaac cattcattta tcagcctggt acactttcag tcaagagtgt
     1681 ggggacaagt cctacaacgg tcactccatc tatttccata gaaggaaata ccatcaagag
     1741 caatcagatc tatttaggaa aagaccaaga aatccaaatc cattaccaag tgagaatcca
     1801 aacagaaaat gaggacttcc atccaaattt ctggtatcaa atgaacggca ggacaacttt
     1861 ccagccaaac attgatacca atgaattagc tgaattcggt ataccatctg ctaaagctcc
     1921 cggagtcagt cttcacatca aaaagttatg ggaagaattt gacaacaatc tagctgatcg
     1981 tccagatcaa gttacttttg agattcaacg ggaacatacg acaaatgctg cagcttggaa
     2041 aaacggatat attcgaatca ttaaaccagc taaagataca acaaatacgt gggaacgtgc
     2101 agacattgac aaattatctg caaatagcgg agaaagttat caagagatat tatcactacc
     2161 tcaatacaat aatcaaggtc aagcattcag ttaccaaaca atcaaagaat tacctgtacc
     2221 aggatacgat tctcaacaaa tagatgcaat gacatggaaa aatactaaac aattcacacc
     2281 gttaaacttg aaaataacga aaaattcctc tacaggtgaa aaggatctta ttggcgctgt
     2341 tttcaaatta acaggagatt ctattgatac tttactaaca gatcatggcg acggaaccta
     2401 ttctcttcca gaaaatgtca aattgcaaaa agaaatgacc tatacgctga cagaaacaaa
     2461 agctccagaa gggcatggat taagcaaaaa gactacttgg gaaatcaaga tcgcttctga
     2521 tggtacggta accattgatg gaaaaacagt cactacttcc gatgatacga tccagttgac
     2581 tattgaaaat ccttttgttg aagttcctgt agcagtacgt aagtatgcga tgcaagggac
     2641 ggacaaagag ataaatctta aaggagcagc attttcccta cagaaaaaag aagcaaatgg
     2701 tacttatcag ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca gttttgattc
     2761 actcacacct ggtaaatatc gagtcgttga aacagctggt cctgccggat atgatacttc
     2821 gccgggaaat tatgaattcc aaatcgataa atatggaaaa atcatttaca cgggaaaaaa
     2881 taccgagatg acaaataatg tatggacgct cactcatcaa aatcgactaa aagcgtttga
     2941 tctaacggta cacaaaaaag aagacaacgg acagacatta aaaggagcaa aattcagact
     3001 gcagggacca gaaatggact tagaatcgcc aaaagatgga caagaaacag atacctttct
     3061 attcgaaaat ttaaaacctg gaacttatac gctgaccgaa acttttacac cagaaggata
     3121 ccaaggtcta aaagagccag ttactatagt tatacacgaa gatgggtcaa ttcaagtgga
     3181 tggacaagat catgaatctg ttctgtcacc aggagccaaa aacaaccaga tttctttaga
     3241 catcacgaat caggcaaaag taccattacc tgaaacggga ggaattggcc gtttaggaat
     3301 ctatctagta gggatgattg gttgtgcgtt ttctatttgg tatctttttt tgaaaaaaga
     3361 aagagggggc agctaaatat gaaaaaactt ggttggctta gtatgtgtct cttcttgtta
     3421 ctatttaaac cagcttttac tcaggtagca acagaaacag aaacagaaat ggttcagatt
     3481 actttacaca aattgctttt cccaaacggg caactgccga aaaatcatcc aaatgacgga
     3541 caagaaaaag ctttattaca aacgtatcga ggattaaatg gtgtcacatt ccaagtttat
     3601 gatgtcacag attcttttta ccatctacgg gaaaagggca aaacggtaga agaagcacaa
     3661 gcagagatcg caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca
     3721 actcttaaca acgaagatgg tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa
     3781 agagataaag cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca
     3841 gagaatatgg tagttgttct tcctgtacat ggacaaaaca atcaaaaact ttcaactatc
     3901 catttgtatc ctaaaaatga agaaaacgac taccctgatc caccttttga gaaggtatta
     3961 gaagagccta gaaatgattt tacgattggt gaaaaaatca cttattcctt gcatacgaca
     4021 attcctgtaa atatccttga ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca
     4081 ttaacgtttt tacctaatag tttaacgatt tcatcgaatg gagaaaagct gacagaaggc
     4141 tttgtcatac acaagaaacc tcacggattt gatgttttat tttcgatccc ttcgttggaa
     4201 aaatatgctg gaaaaaaact gaccatttct tatcagatgc agctaagcag tacagcacag
     4261 gcgaacaagg aaatcaacaa caacggaaca ctggattttg gttttggtgt cagtacaaag
     4321 aaagtctctg tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat
     4381 aaacgattag ctggcgcagt attccttatt aaaaacaaag caggaaatta cctccagcaa
     4441 acagccaacg gatacaagtg gacaaagaac gaatcagatg cgcttcacct gatttccgat
     4501 aaaaatggcg ctttttcaat ttccgggttg aaaacaggaa gttatcgatt aaaagagatc
     4561 gaagcacctt ctggttatat tttaagtgaa acagaaattc cgtttaccat ttcaactttt
     4621 ctttctgagg ataaagaggc ggacagtata ttgaaagtag tcaataaaaa agaaaatagc
     4681 cgtccatttc ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg
     4741 gtattcgcaa gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga
     4801 aaaatcataa aaaaataaac gttatgttag gagtcctttt ccttatttta ccattactca
     4861 caaacagctt cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa gtcatccttc
     4921 ataaaaagaa aatgactgat ttacccgatc ctttaatcca aaacagcggg aaagaaatga
     4981 gcgaattcga tcaataccaa ggattagccg atatttcatt ttcagtttat aacgtcactc
     5041 aagaatttta tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc
     5101 agtctttgac tcctggtaca ccagttgctt caggaacgac agatgctgat ggaaatgtca
     5161 ctttatcttt acctaaaaaa caaaatggga aagatgcagt ctacacgatc aaagaagaac
     5221 caaaagacgg agtgtcagct gccgcaaaca tggttttagc tttccctgta tatgagatga
     5281 tcaaacaagc agatggctct tataaatacg ggacagaaga actagatact atccatctct
     5341 accctaaaaa tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg
     5401 aaaacgaagc actaaatgga gcagaattta ttatttctaa agaagaagga acaccaagcg
     5461 tcaaaaaata catccaaagt gtcacagatg gattgtacac ttggacaact gatcaaacca
     5521 aagccaaaca tttcattact ggtcattctt atgacatcgg caacaatgac tttgccgagg
     5581 catctattga aaaaggccag ttgatcgtta atcatttaga agttggaaaa tataatttag
     5641 aagaagtaaa agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg
     5701 agatcctggc aaatagccaa acaccagtag aaaagaccat caaaaatgat acgtctaaag
     5761 ttgataaaac aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat
     5821 atgagatttc tgtcaatatc ccattaggta tcgctgataa agaaggaacg caaaacaagt
     5881 acacaacatt caaacttatc gatactcatg acgctgcttt aacatttgat aatgattctt
     5941 caggaacgta tgcttatgcc ttatatgatg gaaataaaga aatcgaccca gtaaattatt
     6001 ctgtcactga gcaaacagac ggattcacgg tttcagttga tccgaattat attccttcat
     6061 taactcctgg cggtacattg aaattcgttt actatatgca tttgaacgaa aaagcagatc
     6121 caaccaaagg attttctaac caagcaaatg tcgataacgg gcatacaaat gatcaaacac
     6181 caccgtcagt cgatgtcgtt actgggggca aacgatttgt taaagtagat ggtgacgtta
     6241 catcagacca aacacttgct ggagcagaat tcgtcgttcg tgatcaagat agtgacacag
     6301 cgaaatattt atcgatcgac ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat
     6361 cagcaacggt ttttacaacc acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg
     6421 gcacgtacta tctggaagaa acgaaagcgc cagaaaaata tgttccatta acaaaccgtg
     6481 tagcatttac tatcgatgaa caatcttatg taacagcagg acagttgatt tctcctgaaa
     6541 aaataccaaa taaacacaaa ggtacacttc cttcaacagg cggtaaggga atctatgtgt
     6601 atatcggtgc aggagtagtc cttctactga ttgctggact gtactttgct agacgcaagc
     6661 acagtcagat ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc
     6721 tcctttttct tttattatca aacagtcatc acaaggagaa cacaaatgaa accaaagaaa
     6781 aggaaacttc tagatctgct gatgcttttc ttgcttctct caggaatagg gatactagct
     6841 tatccatttg ttagcgacgc gctgaataat tatctggatc agcagatcat ctctcattat
     6901 cagcaacaag cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga aaacatgaca
     6961 aagaaaaacc agcaattagc aaaagaaggt ggaaatccag gtgcagatcc ctttactaag
     7021 aaaaaagaac ctgcgaaaaa ggatagaacc tattttgaga aacatacgat cggtattttg
     7081 acgatccctg agatcaatgt ccatttgcca atttttgatg aaacgaatac tgtgctgcta
     7141 gaaaaaggtg cctcattatt ggaaggaacg tcttatccta cggggggaga aaatacacat
     7201 gccgttattt ccagccatcg aggactgcct caagcaaagt tgttcacaga cttaccgaaa
     7261 ctgactttag gtgattcttt ctatatcgaa atcaatggac ggacactcgc ttatcaagtc
     7321 gatcagatca agacggtaga accgactgat acaaaagatc tgagaatcga aaaagggcag
     7381 gatttcgtga cactcctaac ttgtacacct tatatggtca acagtcatcg cttacttgtc
     7441 agagggcacc gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc
     7501 gagaacaaac gatttttcct gtatggattg ttagctgcat gtatactgat tttcctcctt
     7561 cttatatatc tttttcgtca atcgttcaaa ataagaaaca gaagttagct attcctcatt
     7621 tctataatat atctattcga atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa
     7681 acttcagcct gtttttatga tgatatcaaa gagagcctat caaacactat ctttcttatt
     7741 actaaatgag gatcttttct taagttttcc gatattcttt aagaaggcct ttttgcatca
     7801 attcttgttt gtttttcaag ttgcggatat tctcttcaaa attttgagaa tatatcactg
     7861 agaataacgt atttaatacg taatcgaagg caatttgaga ggagaaagtc gctactttag
     7921 caaaatcata ttcctcctgc gatataacaa gacacatatc agcctgtttg gccatttctg
     7981 aatgctgatt tccggtaatc agcaaggtag gggcctctac atgcttcaaa taggtcatta
     8041 ttcgttcata atgatggatg cgaccgctgt agctaataaa aatggcgcaa tcctcgctag
     8101 tcaaattggc tgcattccaa gaagaatcac tgtattcttc agccatgatc aaaaacttat
     8161 tcaacttgac cattttattc tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa
     8221 aaagaaagat tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt
     8281 caatctgcaa ttgtgctttt tttattgact ggatcgataa atccgccatt ttctttgcaa
     8341 tttgctgagt gctatcttca cttgaaaaag gaaaattagg atcaatcgtt tgattgaact
     8401 gagcaatcaa ttgatgaact tcccgcccca gctcgaaacg gaattcttta aatccgctga
     8461 atcccatttt tttgcataaa cgaatgatag ctgaatggga agtatacgta gcttttgcta
     8521 attcttcaat cgtcatgtag actgcagctt cgatattcct acggatgtaa tcgacaatcc
     8581 gtttctctgt cgtggtaaac aactcttgtt ttgttagttt ttcttcaata agcatgatct
     8641 gacctccaaa ttctatctta ctgataaaca cttggttccg ctactatctt tttgattttt
     8701 atccttactt tgaacattaa tttcaccaaa ccgaatgata acaagtgttt ttctgaactt
     8761 ttttaccaga atagctatta taaatacttt ctgcgctttt ctaggatata ctgaaaacat
     8821 caataaaagg aggcaccact catgcttaca atcgcttatc ttggcaatgg aaagagtaca
     8881 aatcgctatc atttgccttt ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt
     8941 ttttccccat ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg
     9001 tctgatcttt gggatgatcc tgagatccaa ttagttgtca tcactacacc tagtcaattt
     9061 cattatgaat atggaaaaat ggcactcgaa catggtaaga atgtactagt agagaaacct
     9121 tttgcagaaa cgtcaaagga agcagaagag ttatttgcat tagcaaaaga aaaaggtcta
     9181 tttatccaat gttaccaaaa cagacggttc gattctgact tcttgacagt ccaaaaagta
     9241 atcgaaagcg gaaaattagg ggatatttta gaagtagaaa tgcattatga ttatttcaga
     9301 ccggaagtac cagaaaatac gcatgaattt tctactgcga acagctattt atatggacat
     9361 gcttgtcata cagtagatca agttctttct tatttcggcg aaccagatga tatcagctat
     9421 gatgtacgtc aacttttagg gaaaggacga atgaatgatt acttcgatct ggattttttc
     9481 tatggaaata tgaaaatttc gataaaatcc agttatttcc gcatcaaaga acgtccaagt
     9541 ttcgtcgttt acggaaaaaa gggatgttcg tgaaacaaac aaaagatcga caagaagaac
     9601 atctgaaaat gttttacatg cctgatcatg aagactttgg tatcgatttg cccgaacatt
     9661 atggtgtatt gacctatata gatgaacaag ggagttatca tgaagaaaaa gtaatttccg
     9721 aggttggtga ttacagccgt gtctacgaag ggatttacga gacattagtt aatggcgcag
     9781 aaaaagtcat caaagatgaa gaaacaatct tacaaatgaa aattttggaa gaaggaatca
     9841 aaagcattca agaatcagaa ggaaaaatat gacatatttg taagaatcgg gtttgttttt
     9901 tgacgcataa actgtcttat tgtacgatga aaaagtatag aactactagg aggaaccaac
     9961 atgacaaatt atgacggtga aaaacacaaa cgattaagag gttcaatggc ccctaaaaca
    10021 gaagaagaag tggctttagc aggaactaat tctggtgaaa cagaagacaa agaagaatca
    10081 ttagctggca caaactctgg tgaaacagaa aataaagaaa cagctttatc tggttcaaat
    10141 tctgtagaag aggaaacaaa gcctaatgag cccaatcact ttgaaaaaga tatcgaaaaa
    10201 gcggttgaag atgtcaaaga aaaatttgaa gaactgacct caaaaaataa acatgatcat
    10261 cattgatcgg acgaaatcaa ttcataaaag caggagacat ttggtcacct gctttttttc
    10321 tgtatccatt cgatagaaat cgttcggtac agtcgtaaaa aaaatccaac tgtaagttgt
    10381 accaaacacc cctcgattta aaagcgctac catactagaa tataagtagg aggtgaacag
    10441 cgctgtgaac tacaaaatta gttatctcct gaaaacacca agagaaatca gtctagaaaa
    10501 agcagcagag atcatcgaag taaatacaag cgaggcgatc aaacaattaa ctccttttgc
    10561 cgaatatgtg gatgataccc gtatccgtat aaagaaattc cgagcaatcg actgggcaga
    10621 aaaatttttg gatattactc gatttgaagc tgtgtatacc gaacaagagc gaagagatct
    10681 tctttatttg atggtttatt cgactgcaac gtctgtttct gtttttcatt ttcaagattt
    10741 tcttcaggtt tctaaaggga cagtcctgtc agatatcaaa aagataagaa atgaattaga
    10801 agtggaagat tgtcagctgt cgtatactag gaaaactggt tttaggctgc tagggtcaga
    10861 agcaatatta agaagagaag caaaaaatcg cactgctcgt ttgatgcagt cacttgctgg
    10921 aagatttggg ctcacgtatt taaccgcaac cctttccatt gattattacg ccaaagttag
    10981 agatttgcta gataaaacga tttcactatc agatcttcga ttcgcaccaa atcgaatgga
    11041 tgaattagct tattatttga gtttgacaac cattcgaatt ggaaaaacat ccatggaact
    11101 ggtaaaagat gctgaactgt tggcttctct tgcagtcact gacattagtc gacaagtatt
    11161 gactggcctt ggctattccg aacatcaaaa ttcggagatt atatttttct ctctttgctt
    11221 agcatcgatc gttgaaggag atgtccagga gcctgcactt gattttttgc tggaatgttc
    11281 ttcagaagtc atccatcgca tggaattcct gatggccgta gaatttgctt cgtttcgtga
    11341 attattgatg aatgtctttt atcacttggt ccctgcctat tttcgtattt cctattcttt
    11401 ttatttacct aatgtgatga tcgatcaaat taagcatcaa tatgcttcca tctacgaaat
    11461 gacaagaaaa gcattacttc cgttagaaag acgaataggg aagagcatcc ctgaagaaga
    11521 gattggtttt ttcactatac tatttggcgg agagatcaga aaagcggacg cagaagaaag
    11581 aaatagaaaa ataagagccg tgatcgtctg tccaagtggc attagctctt cattgatctt
    11641 aaaaagtgaa cttcagcaat tattcccgat gatctttttt acagagacta attcagctga
    11701 ccgcttacac gaggttgacg aaacttctta cgatattatt ttttctactg tacctctcac
    11761 acttactgat aagaaaaagt ctctctatgt tctgcatcca atcatgactg acttggaaaa
    11821 aaatcagctg atcaatcaag tacaaagaga ttggctgata ccaggatttt caatgccttc
    11881 tgcccaagaa ttgttgtcgg cgctactgcc ttatatcgaa ttaaaagaag gcgtagacga
    11941 agaaaagctc tatcaagtat taaatcgtaa aatgaacaaa cttttagaaa agaaggagga
    12001 tcaacgtcct atgctatctg aattattaac agaagaaatg atccaattat cggatcaacc
    12061 aaaaaactgg caagaagcaa tcacatttgc cgcacagccg ctattatcag aaaataaaat
    12121 agaagaaagt tatatcaaag ctatgatcga gcgcgtggag cagtatggtg cgtttatcca
    12181 tattggcgat cacatcgcat tacctcatgc tcgtccagaa gatggcgtaa acgaagtagg
    12241 gatgtctttg ctgaaacttc aacaacctgt ggatctgctt gataatccag cccatcccat
    12301 tagtttgttc atctgtttag ctgctatcga taatgaggca catcttcggg ctttggcaaa
    12361 tttaacaaag ctactttcta ataaacaaaa tcttcaagat ttattacacg catcaacaaa
    12421 agaagaaata ttagcgatca taagaaaagg agacgaatga tatgaaaatt tttgcagtat
    12481 gtcaaagtgg attaggaaca agttttatgg tagagatgaa catcaaacaa gcacttgcag
    12541 atcttggagc agatgtggac gagtttgaag taagtcatac ggatgttgga agtgtatcta
    12601 gcgatatggc tgattatttc ttcattgagc attctttgaa agatacgcta acttctatac
    12661 ctgatgaaaa gctcgtacct ttgcaatcga tcattgacag cgaagaagta aaagaaaaag
    12721 taacgaagat cttgtcaaaa gaataaaaat gattcaaggg aggaactatc aatggataaa
    12781 ttgttgaatt tgtttgtgtc aatcgtcagt cagccctcca tcctcgtttc tttgattgca
    12841 ctgcttggtc tcgttcttca gaagaagaaa ttttcagaag tgatccaagg aactatcaaa
    12901 acatttgtcg gattcctagt actgatcggc ggtgctggtt taatcagtaa ttcactaacc
    12961 ccttttgctt cgatgttcca aacagctctg catactcaag gagtagttcc tagcaatgaa
    13021 gcagtagtgg cgattgcttt gcaacaatac ggtacaccta ctgctttgat catgctagta
    13081 gggatgatcg tgaatatttt acttgctaga ttcacaagat ataaattcat ttttcttaca
    13141 ggtcaagcaa tgctttatgt ttcttgtcta acagcagtca ttttagtaag cgcggggttt
    13201 actgcaaatt tgtggatgat cttattaggc ggggtcttcg aaggaacact attaactatt
    13261 acgcctgctc tttgtcaacc gtttatgaag aacatcactg gaaacaatca agttgcgtta
    13321 gggcataccg gaaatatcgg ttatgcttct agtggatgga tcggaaagtt ttttggaaac
    13381 aaagaccgtt ctacagagga cataaagatt cctaaaagtt tcagcttttt gcgagattct
    13441 actgtatcga tcatgctctt gatgtcgatc gtatatgtcg cacttgcttt gttagctggt
    13501 cctggatttg ttgaaagaga actaagcaat ggagacaacg ctgtaatctt tgcactgatc
    13561 caagctggaa cattcacagc tgggtttgtt atcgtccttc aaggggttcg aatggtgttg
    13621 gcagaaatcg ttccagcatt ccaagggatt gctaaaaaac tcgtacccga ttctaaacca
    13681 gcattagatg ttcctatcgt ttttccatat gcacctaatg ctgtattaat tggctttttt
    13741 gttagcttct ttgtaggaac actatccatg tttgctatga taggcttgca aactacagtt
    13801 atcattcctg gggtagtagg acatttcttc tgcggagctg cttctggtgt cttcggcaac
    13861 tcgactggcg gtagaagagg ggcggtatta ggttctgctt tcaatagctt attgattagt
    13921 tggctgccat tatttatcct tccagtatta ggagatctta aaatcgcttc ttctacattt
    13981 gccgatactg attatctagt accaggactt gtacttggga aactaggaga atacggtcaa
    14041 atagccttga cagttggaat catcgctttc tttttacttg tcgtattatc tagctttctt
    14101 tttgccggaa aaagaacaaa cattgtttct aaagaacaga attaagcaaa cttcaaaata
    14161 aaaattgctt ggacaatatc agaagaagat ttaaagtctt ctgatatgtc gcaagcagtt
    14221 ttttgtttta tgcatttatt tttgaatagc ttgttaagac aagtggctgt tttgctagaa
    14281 gaggataact tttaccattc attttcactg ttccgcctgt tctatcagaa gtaatcacga
    14341 tcttatcttt tgttttttca agcgaaatct ttccatcttt gataggtagc gtacaattca
    14401 gtgtttcaaa tgaatctaga ttaggttgta tcataaattc ttcataacct ggctttgttg
    14461 gatacaaacc gataaaatag cgtcctaaaa gatagattgg gcttgctccc catgcatggc
    14521 ataagctttt tccataagga tctccataca tactgtactt ttcatttcct gtttgagtag
    14581 ggtcgtattc ttcccagaaa gttaccgcat catgagataa catccctccc caataatcaa
    14641 gaatcgtttg gtagacgata ttcgtctgtc cgagtttgca taaagcatct tgttcataga
    14701 atttgaaata tggagtagta atctgtgtga tagtgtcatt caataagaca ttctttaaga
    14761 tcgattgctg ttggtctttg ttagcaaaat cgaataaaac agcgaaaata ttcgcatgac
    14821 gcgtcacatt tcttctccca gattcatagc aatcgataaa agcgccaagt tcttcatccc
    14881 aaaagtagtc aaataggttt tgtttcaata gttgatattt ttcttgatag agatgagtat
    14941 gataattttc gttctctcca tatgtttcag ctaagatatc tccgcaagtg atcatggaat
    15001 gataggcttt cagtaataag acttgttctg cagcaagcgt tccttcttta tccatttcag
    15061 accaatcaat aaatatccaa tcttttgctc tcccataaat aaaccctaat tcattcgttt
    15121 gctccatcaa gtatctcatt aatgattgca tcttagggta gacgatgcgt aaaaattcat
    15181 agtctttcgt catcatatag tggttttcga taccaatcaa ccacaagatc gaatagtcaa
    15241 cgatagtatt catatgctgc ttgatttcat tttgtcctct taatgctaat atcgttcgtt
    15301 tattgatttc ctcgtcaaaa aaaagatatt gattgatcat gaatgattga tacgcatctc
    15361 cagaccaaat ccagcgatct cgtttgatac cgtcgataaa aaacaaatca ctacataact
    15421 tgaaggcttc ttctgatact gaccagatct gattcagtaa agaagaatcg cttgaaaaag
    15481 cagcttgttt cgaaagagat aaaaattcat gaaaaacttg aagttgaacc attccagtag
    15541 aaacctctgg tataaacaaa tagcgaaaag cgcgtttacg agctttcatt tttcttgtca
    15601 ttcctatttg cttatagtag caataatcga tatccatagc ttctacatcg gactctccat
    15661 aacatagagt gatagattcg atatggtcat ccagagatat tagttctaca cagccattca
    15721 ctgctcgccc aaaatcgaat aagattcctc cgccaatctc cgtttcagtg actggatgga
    15781 cgcattcttt ttgataatag atttgatttg gatcttttgc gatatcgaca taaagcggac
    15841 tccagccagc tggatattct tcaataaaat tacttgctgt ccagccaata tctgatttta
    15901 cttcgtctcc ctcgataaac atcgctggta gccctgatgt atgtcctaca aagatacgaa
    15961 tttttgcttt accagctgga caagtcagcc attttccaca tggatgtttt tgtccattaa
    16021 tttctacata cccaactcct tggatcgtca ctttaaacat ggaaggttga tcaagaaagt
    16081 aatatctctt aaacttgacg ttccgatggc aatcatccat gtaccagtac gctggccagt
    16141 caaaacctcg ttcttcccgt tggaaatttt gtagcagtcc atggcggatc tcaaagtctc
    16201 caggatacca aagccatcta tctttatgtt tttcatttgt cattttacac gacctccatc
    16261 aaatttattt tgttgtcaat tgttctttta tttcttcatc tactcttatc actgtctttc
    16321 tattcgtcct gtgtactact agaaggctaa taagcgcgct aatcaacagc aagatactgc
    16381 cattgacgaa aacagcaagt gttttactcc caagagaagc aaaagagaga aagagcaaac
    16441 catatggtgc aatgaaagta cctaaattag cgccaaccaa taagattgaa gtattgaaac
    16501 tctccgcgta cttgggcgct tggatactta cttggttgaa taagtaagga atgaataacg
    16561 aaaaagaaat tcctgttagg atcgcaccaa acccaatcac atacaggttt atcacagtgg
    16621 caatcaatac aaagccgatt cccatcagta gtaaagaaag tgtaagcata tagcgatgtg
    16681 cttttgtctg aagccagcca aaacaaactc cggcagccat acttgccaat tgcatgattg
    16741 ttaagatatt cgctgcatta gtttcgtttc ctagagaata agtcgtcagg actgtggcta
    16801 atttaatcga tacagcatta tataccacga tgaccagcaa gagaaaaata ccataactaa
    16861 tcattttagg attcgttttg attacttgtc ttgttggggc tgtgccatgc gattgaagtt
    16921 cagttgtttc aggtgtagga acaaacaaaa caaacaagac aagaataggt aagctgatgg
    16981 cgtagactaa aaaggtatat tgccaaccga actgtactag ctgtcctgca acaaatgtca
    17041 ttaaagctgc accaaatcct tgaaatgcac tttgaaaacc aatcatagag gctcgttcat
    17101 ctccgtgata aaaggcagca atcatgctga ctgccaaaga attgatcaat ccaaagccaa
    17161 tacctagtcc agctcttgaa gctaagacaa tactgtaact ttgtgagaag agggggagga
    17221 taccacaaat cgatgcaatc actaatccga tgatcacagt ttgtttttgt ccgattttcc
    17281 tagcaataaa actactaatc aaaacaaaaa tagtgaccat aaaagaggga gcagtcgtta
    17341 ataattcaac agcagataaa gaacgcgtgg cataagtagc tgacatactg ggaattgcag
    17401 acgtgactgc accagcagaa gtcaaaataa gagagactga cagcagagaa aatttcatca
    17461 tctttttatt cataagcatg ttcctttcat ttttgtaagc gcttatcaaa aatatagtat
    17521 gaagtggtta ttctctcaat agcggaatca caggaagttc atgtcccaac catagaaagt
    17581 ctttatgaaa ccaaaaatag ctttgcatat aatagcagta cagcgtgaac taataagcga
    17641 aagaaaccgc ttacaaaaag gagggagtca attggaacta gctgaattag acattttttt
    17701 gcgtaacgaa gaatcaatcg aaactctgca gcgtgaacaa ggaatgaata tcaatgattt
    17761 aaacttagct taccatcttc agacaccgcc aaaaatccac cgaggggatc tatttcaaca
    17821 gggaaatatt catatcagta aacaccgtcg atatgcggaa gttcccgagc atacacatga
    17881 ttttgttgaa atcaattata tgtatagcgg tacatgttta caaattatta atggagaaag
    17941 gatagagatg gaagaaagtg acgtggtgat cattgataaa gaagctactc aaagaatcga
    18001 atacacaggg caagaggata ttttaatcaa catcttatta aaagaagaaa ccttatctac
    18061 agagatattg aatcatttgg caagcacaaa taatctactt tcttcttttt tgataaatgc
    18121 atcaaaagaa aaaggggaac atacacaatt cattcatttt cgcggcggaa aaacaagtcg
    18181 tttgacgcat ttgattgaaa atatgctgtt gatttatttt gatacataca gtcataagat
    18241 ccgttcgtta aatctctata catcactatt actgatcgaa ttgactcatt tattagaaca
    18301 acaaagcttt tcatcagcag atggatgcgc agaaaatgag atgcttagag tattagatta
    18361 tatcgataag cattttactt cttgtacgtt aaaagaaaca gcagctcatt ttggctacaa
    18421 tccggtctat cttagtaata aattgaaaat gtattttgat gcttcctttc aagaactggt
    18481 tctaaagaaa aaattcgact ttgcgttgga actgattcaa gaaacgagtt tttcgttgga
    18541 agaaatcgct aatcgtatcg gatataaaca aacgacttct ttattcaaat tattcaagaa
    18601 gtattcaaca tgtactccca atgaatggag aaataaacat taagaagaaa atctttcact
    18661 aaagcatatg aaggaaaatt gttttaggtg cgattgctgt cttattatat cgttgcagcg
    18721 aatttttttg gatttatttt cagttctaaa cttttggttg tattatcagg ggatataggt
    18781 gtaaacattt gtcatttatc tgttatgcgt attcaggaat tcgtttccat caaaaattat
    18841 aataacaact attataatta acatgttgat aaaatgaata aacaagacta tacttttcat
    18901 gaaagtaaca aatagttatg aaaaggagct gtttattcat gaaaaaaaca agtcgtaaag
    18961 tagtaatcgt cggtacaggt tttgttggaa caagtatcgc ttatgcaatg atcaaccaag
    19021 gaatctctaa tgaattagta ttgatcgatg tgaatcaaga aaaagcagaa ggggaagcat
    19081 tggacctttt agatggtatg gcttggggcg atgaaaatgt ggctgtatgg tcaggtggct
    19141 atgaagaatg taaagatgct gatatcgttg ttatcacagc tggtatcaac caaaaacctg
    19201 gtcaatctcg tttagattta gttaaaacaa atgcgtcaat catgcgccaa atcgtaaaag
    19261 aaatcatggg atcaggattc gacggtatta tcgttgttgc ttcaaaccca gtagatattt
    19321 tgacatatat cgcttggaac gaatctggtc tgccaacatc acgtgttatc ggtactggaa
    19381 caacattgga tacaactcgt ttccgtaaag aaatcgcatt gaaattaaaa gttgacccac
    19441 gcagcgtcca cggctatatc ttaggtgaac atggtgattc agaagttgct gcatggtcac
    19501 atacaactgt cggtggtaaa ccagtgtttg aaattgtaga aaaagaccat cgtattgccc
    19561 aagatgaatt agatgtgatt gctgataaag tccgtaatgc tgcttatgag atcattgatc
    19621 gtaaaaaagc aacttactat ggtattggta tgagtactgc acgtatcgta aaagctatct
    19681 tgaataacga acaagctgta ttgcctgtat ctgcttattt aactggtgaa tacgacgaaa
    19741 aagatatctt tacaggtgtg ccatcaatcg ttgacgaaaa tggtgttcgt gaagttgttg
    19801 aattatcaat caacgaagaa gaaaaagcaa tgttcagcaa atcaacaagt gctttaagag
    19861 aagtattgaa cactgtatta taagaaacaa tgaatatgaa ctaaaaatcc taaaactgct
    19921 taacagctga aatagctgtt aagcagtttt ttattgatat ctaataggta agcaaataaa
    19981 tagaatggtt aaaattaatc ggtccgaaaa tgtatccgta catatgaaca tatgaaattt
    20041 aatctgttga ttacaataag cgaatcgtta tcaatgttta tctgtggtta attaaaaaat
    20101 ccttccgcaa cggcaaatac aatgattcat atctaattcg ttcatatttg attaactgaa
    20161 taaagaaact ggtttttatc attcaattaa acgacaaaaa aagtaagaaa aactgcaaaa
    20221 tttttttata attttgcata agcagatttt gatggaaaaa tgacgatttt tgataaaaaa
    20281 ccgttcgaga gaggtttatc attttt
//
LOCUS       DADQRX010000050        20238 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.051, whole genome shotgun sequence.
ACCESSION   DADQRX010000050 DADQRX010000000
VERSION     DADQRX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.051"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(184..1434)
                     /locus_tag="J8V14_001684"
     CDS             complement(184..1434)
                     /locus_tag="J8V14_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBA0921790.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(1812..3263)
                     /locus_tag="J8V14_001685"
     CDS             complement(1812..3263)
                     /locus_tag="J8V14_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBA0921791.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(3584..4348)
                     /locus_tag="J8V14_001686"
     CDS             complement(3584..4348)
                     /locus_tag="J8V14_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBA0921792.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4376..5074)
                     /locus_tag="J8V14_001687"
     CDS             complement(4376..5074)
                     /locus_tag="J8V14_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBA0921793.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5086..5865)
                     /locus_tag="J8V14_001688"
     CDS             complement(5086..5865)
                     /locus_tag="J8V14_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBA0921794.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(5881..6825)
                     /locus_tag="J8V14_001689"
     CDS             complement(5881..6825)
                     /locus_tag="J8V14_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0921795.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6871..7650)
                     /locus_tag="J8V14_001690"
     CDS             complement(6871..7650)
                     /locus_tag="J8V14_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921796.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(8030..10105)
                     /locus_tag="J8V14_001691"
     CDS             complement(8030..10105)
                     /locus_tag="J8V14_001691"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBA0921797.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(10107..11024)
                     /gene="glyQ"
                     /locus_tag="J8V14_001692"
     CDS             complement(10107..11024)
                     /gene="glyQ"
                     /locus_tag="J8V14_001692"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBA0921798.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(11410..12228)
                     /gene="recO"
                     /locus_tag="J8V14_001693"
     CDS             complement(11410..12228)
                     /gene="recO"
                     /locus_tag="J8V14_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBA0921799.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(12371..13270)
                     /locus_tag="J8V14_001694"
     CDS             complement(12371..13270)
                     /locus_tag="J8V14_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBA0921800.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(13285..13686)
                     /locus_tag="J8V14_001695"
     CDS             complement(13285..13686)
                     /locus_tag="J8V14_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBA0921801.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(13664..14140)
                     /gene="ybeY"
                     /locus_tag="J8V14_001696"
     CDS             complement(13664..14140)
                     /gene="ybeY"
                     /locus_tag="J8V14_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBA0921802.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(14169..16361)
                     /locus_tag="J8V14_001697"
     CDS             complement(14169..16361)
                     /locus_tag="J8V14_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBA0921803.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(16385..17356)
                     /locus_tag="J8V14_001698"
     CDS             complement(16385..17356)
                     /locus_tag="J8V14_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBA0921804.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            18061..19440
                     /gene="brnQ"
                     /locus_tag="J8V14_001699"
     CDS             18061..19440
                     /gene="brnQ"
                     /locus_tag="J8V14_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBA0921805.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            19722..>20238
                     /locus_tag="J8V14_001700"
     CDS             19722..>20238
                     /locus_tag="J8V14_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0921806.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKS"
BASE COUNT         5596 a         4291 c         3546 g         6805 t
ORIGIN      
        1 tttctattta cttgtctcat gtagtacgtt ttttcgcctt ttgactcagc tgaatattct
       61 gtctgttctg ttataacaaa actctcatac ttctccatca gtggttttaa catcattaac
      121 tgttcataat gccccccaga ggaagccgca aaacatatct ttttgttcat attaacctct
      181 ctatcagtca cgctgaaaaa tatctctagt gtaaactttt tctttaatat catccaagca
      241 ggtatcatat cgattggcaa taatagaatc acacatcgcc ttaaactgct ctaaatcgtt
      301 gataacctta cttccgaaaa aagtctctcc atctttcagt gcaggctcat aaatcaccac
      361 agtcgcacct ttagctttta cgcgcttcat aactccctgg attgaactct gacgaaaatt
      421 atcactgttg ctcttcatgg tcagccggta tataccgatt aaaactggat tttccctgtt
      481 gacatcatac tgattatatt cagcgtagtt gtaatagccc gccatctgaa gaacacgatc
      541 tgcgatgaaa tccttacggg tacgattaga ttcaacgata gcctcaatca aatttccagg
      601 aacatctgca tagtttgcca acaactgctt ggtatctttt ggcaagcaat aacctccata
      661 tccaaaggat ggattgttgt agtgagttcc aatacgagga tcgagacaaa caccattgat
      721 tatctgctga gtattcagcc ccttcatttc agcataagtg tctaattcat tgaaatagct
      781 gactcgcagt gctaaatagg tatttgcaaa cagctttact gcttcggctt cagtgaatcc
      841 cataaaaaga gtatccacgt tctccttaat tgcacttttc tgcagaagtg ttgcaaaaac
      901 atgagcagca tttaacaagc gctcatcagt catatcagta ccaacaataa tacgagatgg
      961 atacagatta tcatataaag ccttgctctc gcgcagaaac tctggactaa aaataatgtt
     1021 cttactcccc gttgtctcac ggactgattc agtgtagcca acaggaattg tagacttgat
     1081 gaccatgatg gtatcaggat tcacgtccat cacaacctca ataactgact caacagcaga
     1141 agtatcgaaa tgctgagtac gactgtcata gttagtcggt gtagcaataa caacgaaatc
     1201 tgcggattta taagcgctcc ttgcatccaa agtagcagtt aagtcaagtt ctttttctgt
     1261 aagatacttt tcaatatatt catcctgaat tggagacttc cgtttattaa tcagttcaat
     1321 cttttcaggg ataatgtcta ccgcggttac ctggtgatgc tgcgccaata gtgtggcgat
     1381 actcaaacca acataaccgg tgccggctac tgcgatgttg tactttttct tcacaatatc
     1441 tcctctttcc tctcttaagt atggagaatg ttactcctac ggctattcat catgtcaaaa
     1501 taacacagct tagaatgttg ataaaataat tttccagaat gtgataaaat ctctttcatt
     1561 tataaaccac acatctaatt ttatctattc tccacaagac atgtagagta ttctttaata
     1621 acaagatacg accttactaa atacttcaaa aaattatcat atttaattca taaatataaa
     1681 tcttcatttt aaatgtatgc ccaaaaattt atctgtaatg cgtcatcaaa atctagattc
     1741 caaatcttta tcaattttta tccactcatt ctaccacttt agtaatattt tctatttata
     1801 acactttcgt cctacattga tccccccctc tgaagtacag tttttactgt cttaaataaa
     1861 attttaaaat ctaaccccat actccaattt tttatatatt ccgtgtcaag ttttgctact
     1921 tcttcaaaat cagtaatatc acttctacca cttacctgcc acattccagt aattcctggc
     1981 ttaatagcaa ttcttgcacg atgatgataa tcatattctt catactctga tttaaggcat
     2041 ggtcgaaatc ctataaggct catctttcct gttagaacgt tgaaaaattg agggaattca
     2101 tctaaagacg tggatctaat aaattgaccg attcctgtct tttttgttcc atctggaagt
     2161 attttatttc cgataattct tggatcaaac tccatcttaa acataagatt attgctcatc
     2221 ttattttctt tcattaattc tacttttctt tcttctgcat ccatatacat acttctaaac
     2281 ttatacatct tgaacggttt accattctga cctatacgtt cttgtgcaaa gaaaattggg
     2341 cctggtgaat taatataaat aagtggagca ataaagataa aaataattcc cgttgcaata
     2401 catccaacaa tacctgcaag aatatcaatt gttcgtttta taaatgcctg ttgagttgtc
     2461 ataacattga tacttgttgt aagtactgta tattctccta tacgaccaat agattgtttt
     2521 cccaaagcac tatctgatac ttttgcaaga ttcaaatgta cagtaaggcc catttcagag
     2581 catcgttcaa taaattcttt catacacgaa gtacttttat tcaaaataat aaagacttca
     2641 tctacccatc cctgacataa gtaatccgct gcatttttgg aatcagaaac aactgtcact
     2701 ccctcaacat cttcaccaat catgctttta tctatgataa tgacaccatt tattttatac
     2761 attttatagt tatgctcttt cacattacga ataactactt cagcaatact actagatgta
     2821 actataataa gtgatttgtt cccctcattt ttcattttat gtttcaagta atgtttccat
     2881 aagaggcgag ttatatatgt aattacaacg tataaacctc ccatcaagaa aagaacgact
     2941 ctagaataat catttccacc ctgaacacta atgagataca aacttcctaa cagcaccaac
     3001 ataatcgctt gctttacaga agaagcaaat tctatatagt accctctttt taatacacct
     3061 ctatatgctt caaacatgaa aataactaaa agatctgcca gttcaatgaa gactgccatg
     3121 ttttgataca ttggaatcac ataaggattc caatctacct ggcgtaagac atacgataga
     3181 taaaaagcaa gctggataca caataaatct aaaataataa aatcaatatg tttaacccat
     3241 ccccttgaac ttttcttgta cattcctttt tactcccctc tttttaataa aataaattgt
     3301 attacttgtt ttctctccta aatatctttc tgaaggatta ttgactcgac aaattggctt
     3361 gtggatattc ttatatatag aacatccgta agccaactta aaactcctta acaaaatttt
     3421 tttactcata ttgtagatca aaacaagtat ccaagaaatc atattttatt tcttttttct
     3481 gcttactcaa caacagctaa aagaaaaatc atcataaaca aattcaacta agccaacgat
     3541 ccccttgtga aatcaagttt tttcttgatt tacgaataaa gagctaaaat aaaccaaact
     3601 tcttcttttt tacctcttca tagactggat aactgatttc atccccgttg atcagatcct
     3661 ttgcgacttg tttcatctgt actacttttt ccttaccaaa gtcctttgca atctgctcat
     3721 agcactcttt taagtaaaag gtgcgcttag agaccccatg ggcatcagaa gcagccatct
     3781 gcacgagatt atgtttgacc atcgttttgg ctgttttctg gatgtctttg ccaaataccc
     3841 caacataact tggcgcagtg agttgggcca aacatcccat atctaggaac ggaagcaaat
     3901 gatttgggtc ttcgcgaaaa tgcacgttgc gttctggatg gacgattact ggtgtctttc
     3961 ctagttctaa caactcaaaa aaaagccgtt ctgcatataa cggcacttcc agtgtcggaa
     4021 attcgatcag aagatatgta tcatctaaat ctgtaaataa tatttcatct cggtggatgt
     4081 cttcgatgag cgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaact
     4141 gtcttttctc taattcggct tgtaacgaag cgactagaga aatgacttgt gatttttcat
     4201 tgctgtattt tccattattg tggtgtggtg tgcataaaat atgcgtgatg ccttgctgaa
     4261 tcgctttttc agccattgca atacttgctt ctaagttttc tgccccgtca tcgattcctg
     4321 gtaggatatg gcaatgtaga tcgatcattt ctcttgtctc ctctcttctt attccttaat
     4381 ttccgtagta ataaccagca tctttgctat gttctgctcc gttgtacaca acacctaaga
     4441 ctcgtgcttg taccatctcc agcagttctt ttgctttagt caaggattct ttacgtgaca
     4501 tattttcacg tacgaccaat aaagtgccat ccgtttttaa agacatgatc tgtgcatctg
     4561 ttacggctac tactggtggc atatcgaata tcaccacgtc aaataactgt cttgcttcgg
     4621 ataggatctg atccattctt ggcgagctta acaattctga tgggttgggt ggcttaggtc
     4681 cactaggcaa aatcgacaag ttctcgactg gcgttcgttg gatcacatct gctacacttc
     4741 ctgatgaact aagtgctgta cttaatccac tggcattgtt cagctggaat gtcttataga
     4801 cgacgggttt acgtaaatct gcatcgacta gtagaactcg ttgacctgat ttagcaaaaa
     4861 cgactgcaat gtttgctgca gtggtggatt tcccttctcc aggacctgat gaagtcacga
     4921 caatcgtttt gatttgctga ccagctgcgg aggcaaactg gatattcgtc cggatcgtac
     4981 gatattgttc ggcgattggt gaagatgggt ttgttaatgt gatcaaactg actgcatgag
     5041 tttgtgctgc tttttgtttc tgtgttcgtg ccatttttct tcctcctaga ctcttgagcg
     5101 gcttctgcgt gatggggctg ttttatcgtt tgtagctttt ggtcgtgcag gagctgttcc
     5161 ttttttacgg ataccagcgt tcaattcttt cgctgacatt tctggaactg ttcctaagat
     5221 tgggaatcct aatgtatctg tcacatattt ttcatctttc actgtccgat cgagtaattc
     5281 caataagaat gccagaccta cacctaccat cattcctaac attaagccag tcattaaatt
     5341 caatttatta tttggcgaaa caggtgtcgt attggcaact gcacctgaaa tgatcgagat
     5401 tttatcaaca ttcatcacat cttttgcgtt ctcttggaaa acttgtgaag ttgtgttagc
     5461 gatctgttga gcagccactg cattcgtact tattgcctga atcgagaaca tttgtgaatt
     5521 ttctgcctga ttaactgaga tggcatcttt gatttgttca gcagtcattt tcacattgtc
     5581 ttctgtttct aaacgatctt tgacttcatt gagcactaaa tcactgacga tcatatcttt
     5641 atacgtattg atcatttgca ggttcgtatt gacatcattg acatttgcag tgtctgattg
     5701 tggcagtgtc acgatcaatt gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt
     5761 tacgattcct gagatggcca agccagcaaa cattgagatg atgattatta ccaagtgttt
     5821 gcgtaatgtt tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc
     5881 ctatttattc tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg
     5941 atcactgact ttttgatagg agatattatc ttgcatgaag ccttctcctt gcagctgatc
     6001 ttgcttaatc gtaccaaatg cctcccgata atctaaagca atttttttca tttcatcaaa
     6061 gttcatgtct gttttcatat tcgactctag agcattcaaa acggattgat agttgctgac
     6121 accgtttaga ctcaatactt ttttcgctac gccttctacg atctttcttt ggcgcccttg
     6181 tctaccgtaa tcaccatttg ggtcttcata acgcatacga gaataagata atgcctgatc
     6241 cccgttcaat gtaattttac cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc
     6301 attattgact tcgataccac caactgcatc aacgagttcc tttagtcctt tcatattgat
     6361 agagacatag tgatcgattg ggatgtctac atatttttgt acggtatcca tcgccatggc
     6421 agctccgccg aatgcgtaag cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc
     6481 gacatacgta tctcgaggaa tgctgacgat cgttgtctgt ttgtcttcag gactgactgt
     6541 ggcaaccatc atcgtatctg atcttccttg atcgacccga cctaaatctc cagtatcaat
     6601 ccccatcaat agaacagaga aagattcttg tttgtccaga tcaactggtt tcgttcgttc
     6661 tccttgggtt cgttccactg attcgtaagt tttctcaata gaatgtgaga gatccagata
     6721 taattttgct cctactccta caattgctaa tactaatacg ataagaactg ctaaaataat
     6781 caaaaatatt tttttccctc ttgattttct tcttcgtctt gccatagttt cctccgtttg
     6841 ctaaccttgc ttgaatcaac gttttaactg tcaatagctt atattgctat agcccatttg
     6901 ctgagcaaca gtttgtgctt ttgttgcgta tgcggctctt attttttcat tccggatctg
     6961 ctctatttga ctgagcgccg tcaaatattg atcgtaagta gcggtagctg ttacttgacc
     7021 gtctttcagc atcgtatcga atagctgatc tagttgcgtg acttgtgcaa tttgggtatc
     7081 agcgaatcct agatattgca taacgactgt tttccaagca ctatccttaa aaaagctcat
     7141 attttcacgt acatgggcga catctgcact tgccaacttt tctttgatga tgacatcatc
     7201 cacagcttgc tgccagtttg atacgttttt agtaaataat ttattgacag ccgtctggat
     7261 cttcagtttt gattctgcat cctctaactg cgttaagact gctttctttt cttcttggat
     7321 cgcagcagct tctttaggta attctgattc cttgatgtca taatcatctg ccgaaagttt
     7381 gatagaagat agtttatgtg acaaatttga aactgtttct tctgttattt caggatttag
     7441 gaaaattttt tcttttgtac tgtacagctt atctacttct tttgataaag aggtcacggt
     7501 gtctttttga ttggtgatgg cagattctgc gtaattgatc cgttgatgat attgccgctg
     7561 attattgaga tggtatccaa acaagcaaat cacaatagcg cctgtgccta ccgccacagt
     7621 agccaataac ttgacgaacg gcttttccat agattcactc ctaaatattg atgatagata
     7681 agtaaagatg tgattcattc acatcagaat gagcgctttt tattttaaaa gcataattaa
     7741 actaatatat tcatagaaat atttataaac aggttttccc tatataatca taccacatac
     7801 agtttttttg actagggaaa ttcctaattt aaaagtaaat aaaacagtaa caagattata
     7861 attaatcgtt ttcattttta tttcctcact tttcatgagc gttttgaaaa ttcgaacaaa
     7921 gaataaaata acaaaaatta ttaaaataga attaataaca gctatttata acaaaaaaaa
     7981 cagagacaca gcacgtttcg tgcatgctct ctgtttttca aacaaaagac tattttgtga
     8041 tgatttgatc caagcttgct aatgacaaag ccatggcagc tatttgactt agttgtgtta
     8101 gtcggttctt acgtactgct ggttcatcag ccatgaccat cgtttgatca aaataatttt
     8161 cgatcagtgg acgaagagcg acaagctggt cgtagttttc gtggatcgtt tggtcagcaa
     8221 aacttgcttt cattgcttct actgctttgt gtaattcttt ctctgcgtcg ttttcgaaca
     8281 gctcagggtc tacttctgat tgtttttcaa gctgcgcttt tttcgctaag ttcacgacac
     8341 gtgtcaaagc ttccattgaa ggtttgaagt cttcatctgc aagatgttgt ttgaacaatt
     8401 gtgctgctgc aaatactttt gtcagatctt tttgttccgc attcaaaaca gcatcgatga
     8461 catcatgacg aatattcttc gttgtcaata attggcgcag acggcctttg ataaagtcga
     8521 tcacttgttg ttgcccttct gataaaccaa tgccgaaacg atccgtatct ttactgataa
     8581 catcgctaat cgtttcttgc agaacagaca aaggaaaggc ccattttttc gattcgatga
     8641 tccgaacgac accgtatgct tggcgacgta aggcataagg atcgtttgac ccgcttggaa
     8701 tcatcccaac tgcaaagaaa gaaaagatgc tatctaattt atctgcaatc gctaaaacag
     8761 aacctactgt agactgaggt agctctcctt cactggatat aggcatgtaa tgctcacgaa
     8821 tcgctgtcgc tacagcaggt ttctcgcctt tcagaagagc atatttttcg cccatgatcc
     8881 cttgtagttc aggaaattcc cctaccatgt tggttactag atcaaattta tagatttcag
     8941 cagctcgttg caaatccatc aattcttcct cagatagtcc tacttgttcg ccaatgatac
     9001 gggcaatgac ttgtacccgt tgcattttct catagatact accgattttt tcatggaaag
     9061 taacagtttt tagtttttca acgtattggt cgattgaagc tttcttgtct tcattataaa
     9121 agaattccgc atcttctaaa cgagccgtca atactttttg attgcctcga accacatctt
     9181 ccaattttac ccgatcacca ttacgagcag aaataaaatg aggaagcagc ataccttgat
     9241 cattccgtac ctcgaaataa cgttgatgtt ctttcataga agtgactagt acttcttcag
     9301 gaacagaaag atatttttct tcgaagtcgc cagaaaagac agttggatat tcgactaaat
     9361 tgttgacttc ttctagtaaa tcttcgtcta gatcaataac ccagtgattt ttttctgcta
     9421 attcgtttgc ttgtgtaacg atcatttctt gtcttttttg cggagacgca atgacgaatt
     9481 gttcattcaa tttttcttca tattcttttg catgcgcaaa tgtcacatct tcgcctaaga
     9541 aacgatgacc tcgagaggct ctgcctgttt tgacatccaa gatttcgaat gggattactt
     9601 cgtcatctaa caatgccacg atccagtgaa tagggcggat atattcaaaa tcgtattctg
     9661 cccaatgcat cgttacaggg aaagtcaaac ttttgatcac ccctgctaag cctgtcaata
     9721 cttcttttgc ttccttacca tgagtgtatt tcgttacata gacatattcc acacctttta
     9781 attctctaaa agtgatagcg tctgtcgtca gaccttgtcc gcggacaaat ccttccgctg
     9841 ctttcgtcca gtttccatct gcgtccaaag caattttttt ggctggtcct ttgacttctt
     9901 cttcactgtc cgcttgtcgt tctggaatat ttgttacttt gattgctaaa cggcgcggtg
     9961 tggaaaatgt ttcgatcgta tcataagtca attggttttc atctaaaaat tttgctgttt
    10021 tttcttctaa ttgtttcatg cttggtgtga caacatgggc aggcatttct tccagcccga
    10081 tttcaagtaa taagtctttt gccatcttat tcagcctcct tcacggtttc tggatttgct
    10141 gtgtttttat tcaacaaagg gaatcctaat ttttcgcgtt ctgccacaaa gattttggct
    10201 acagcacgag ccatgttgcg gatgcgagaa agataaccgg cacgctctgt tacggatact
    10261 gcaccgcggg cgtctaataa gttgaatgta tggctacatt tcaagatata atcatacgcc
    10321 ggatgaacaa gattttcatc gatacagcgt ttagcttctt tttcaaaacg atcaaagttg
    10381 cttagtagca agtcttggtc gcttacttca aaagaatatt tggaatgttc atattctggt
    10441 tgtttgaaga tctcgccata tttcacgcca tcagcccatt ctaaatcata gacactttca
    10501 acttcttgga tataagaagc aagacgttct aatccataag tgatctcaga agttactggt
    10561 ttacaaggca gtccaccaac ttgctggaag tacgtgaatt gtgtgatttc cattccgtca
    10621 agccatactt cccaacccac accggcacag cccatggatg ggttctccca gttgtcttca
    10681 acaaaacgga tgtcgtgttc taatggatcg attcctaaaa ggcgcaagct ttctaaataa
    10741 agctcttgaa tattctcagg tgaaggtttc atcaccactt ggaattgatg atgctgatac
    10801 aaacggtttg ggttttcacc ataacgtcca tcagctggtc ggcgagatgg ctctacgtaa
    10861 gcagcattcc atggttctgg tccgattgcc cgtaaaaagg tatatgggct catcgtacct
    10921 gctccttttt ctgtgtcata agcttgcatc agcatgcagc cgtttgatga ccaaaatttt
    10981 tgtaatgtta aaatcatatc ttggacggtc aattttttac tcatctgttt tgcccctttc
    11041 aattttaaag cgttgctgtc aaataagaat acaggaaaga aaacgacaaa aaatccctat
    11101 gtctgattac agacataggg acgaataatt cgcggttcca ccctacttcc gaataaagat
    11161 tcggcagctt catttatcgt actcgcaagt gcctcttcaa aaatcgtttg ggtccggctc
    11221 ccactctccc gaactcgcta aaccacgtgt tttttactga tcttggtcaa tgtacgtatt
    11281 cattttgcag ttttcttgtt tctataatag atcctatcac tgaacgatct atttaatcta
    11341 tggttaataa tagacggaac tccctgtgtt tgtcaaatgg ttttgatttt ttgttgtatg
    11401 ttttatttac tattcttctt tttcaggtgt ctctttttct ttttcagaca tgtctttctc
    11461 tgacgtcttt aatacgtgtg cccaagactt catttgattg atgaactttt tgcttttcaa
    11521 atggataccg acatattcat catacagctg gtcgatgagt tcacggattg cttgtttggt
    11581 ttcttctttc agatggattc cactaatttt atcataagaa atagctgaaa acatccgaac
    11641 gaaataaaca gctcgaggat ctgcatgata acggtgatcg tccatcgacc aatgtctttc
    11701 gcatatcaca ccaccatatt tggaagaata gtcaaatttt ccttttgttt ccccgcatac
    11761 gacgcagtgt gtccagattg gtgcaattcc gaagcgttgc agtatttgga cttcaaaaat
    11821 attcgtgatg atttctgcat cgtctccttg gtccatccgt tgcagtgctt gttgcgtaaa
    11881 ctggaacaaa tgcgggtcgt actgatgatc ttcgattgcc gcatctacta gattcaataa
    11941 atatgtgcca tagccgctca aaaagatgtc ttcttggatg tgtcgaaacg catggacatc
    12001 tttgacactg cttaaaaatg acaaaccttc acttcgaaaa ttcccgatat aaactgcttc
    12061 agtaaaaggg agcaaagccg gagctaaggg ctgattttgc cgatggatct ctttgatata
    12121 aaacatcatt ttgccagaag attctgtaaa gattttgact aatttatctt tttctttgta
    12181 attacggcta gataaaatca agccttttga ttctgcaagc tggctcatat tctccctcct
    12241 aaaaaataaa tctggaaaag cagattctgc cgatccagat catttatgtt gcgatcttcc
    12301 gcagcttttc tcaacacgcg gatttttatt cagtttattt ctatacctaa tttacaagaa
    12361 gctcagaata tcagtattcg tcttttcggt agccgaagtc ttgcagatag acttttttgt
    12421 ctcgccagtc tttttgaact ttgacccaaa gttctagaaa gactttgtct cctaaaagat
    12481 tttcgatatc ttttcttgct ttcgttccta cttgcttcag catttttccg cctttgccga
    12541 taatgatccc tttttggcta tctctttcga caataatcgt agcctggata tggattttat
    12601 cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac agaatgaggc acttcgtctc
    12661 gtgttaaaaa caagactttc tcacggatca attcagaaac gatgaagtat tcaggatgat
    12721 cagtcacttg atcatctgga aaatattgtg ggccctctgg catttcactg actaatacat
    12781 ccatcaaacg ttcgacgttg tttccttctg tagcagagat tggaatcact tgtgcaaaag
    12841 gcatctgcgt tgaataatct tcgatgattc ccaacaactc gtctggatgg attttgtcga
    12901 ttttattgat aatcagataa acaggagaat gcacgttttt tagacgttca ataataaaat
    12961 cgtctccccg accgcgtttt tgatcagcac taatcataaa taagaccgcg tccacttctt
    13021 tcaacgcact gtaagctgct tctaccataa aatctcctaa acgatgcttt ggtttatgga
    13081 ttcctggtgt atcaataaaa atcaactgag cttctggtgt agtgtatact ccttggattt
    13141 tatttctagt tgtctgtgcc ttgtcactca tgatggcaat tttttgaccc acgattcgat
    13201 tcaataatgt ggatttccct acatttgggc ggccgacgat tgcgacaaac cctgatttat
    13261 gttctggcat atatgttctc ctttttaatt aaacaacaaa tgatatattt tagggacaaa
    13321 taaaatcaac ccaacgatga cggtaaaaca agcggtcact aaaactgctc cagctgccat
    13381 atctttgatt tttttcccaa tcggatgaaa atgaaaatcc gtaaacatgt ccacgacatt
    13441 ttcaaaaaca gtattgatga tctctactat ccatacgaga aaaacagcta aaagcagcca
    13501 taaccattct atccgatcaa gttggaaaat cacgcccata aacaaagcga ggattccgaa
    13561 cgcaacatgt ttcctcatgt tgcgttcttc ctcaaaaaca gttttgactc cttggacggc
    13621 aaattccacg gacatcagga aatgtttgtt tttctctacg tttttatctt ttaagtccat
    13681 aggcatctaa gatctctttc tgtaaaccaa acatttcttt ctcatcctct ggtgtcatat
    13741 ggtcataccc gttgatatgc aagaatccat gaaccgctaa aaagccaagt tcccgatcat
    13801 acgaatggcc gtaagcttct gcttgctctg cagcacgttc agtagagatc atgatgtctc
    13861 ccagtacacg aggcaatgga ttctctccat cttcaaaaat cactgggatt tcgtcttcgc
    13921 cctcttcttc caaggcaaaa ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac
    13981 gattgattac ttggattgct gcattgtcca tgaaagtaac agacatttct gtttcttctg
    14041 gcaactctaa atatgatgct gcaaattgca gcaagtcgtc gatttctttc attttctctt
    14101 ctgagacttg gtttgtttca tccatgaagg taatatccat tatacatcgc ttctttctat
    14161 tcttcttgct attcttcttg ttcttctttt atcttctcaa cttccgattt cattttagga
    14221 tatttgatcc gagaatggaa ggtactgctt aaaccgctga tcaatgattt ttctacttta
    14281 cggatttctt ttaacgtcaa tccactgtcg tccaattgtc catcggaaat acgctcttca
    14341 ataagattat ggacaaaact ttcgattttt tcgctcgttg gatggtccat tgcccgtact
    14401 gccgcttcac aactgtcagc tatattgacg attcctgctt cacggctttg aggttttggt
    14461 ccaggataac ggaattgctc ttccgtaact tccgggttgc gttcctttgc cttaacgtag
    14521 aaataacgca tcagcgtggt tccatgatgc tgtctacaaa tatcgatcac catttgcggc
    14581 atattataat cttccaatat tttggcacca tctgtcacat gtccaaagat aatatgttta
    14641 ctgtcttctg gcaataagaa attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg
    14701 aaaaagctgg cgtgtttgat cttgccgata tcatgataat agcaagctac tcgagttagt
    14761 aaagaacgtc caccgatttc tgctacagca ttagcactta gattggctac catcatgcta
    14821 tggtgatacg tacctggtgc ttcttccagc aactgtttca ataaaggatg attgggattg
    14881 ctgagttcat ttaatacgat cacactatca tcattgacca tcaattcaat ataggggtgc
    14941 agacctatcg tcagcaagaa tgaaaacagt gcacctgtga atccgcagac tagcgttaac
    15001 caagtcgttg catccccaaa gctcattcct tggtagataa tcaaaatgac atccatcatc
    15061 acaggaaaaa taatgaccca catagcagcg gataaccctt gttctgagat ccgtttgcgt
    15121 ctgacgatcg ttgccatgag accagaaaat acataggaca tcaaaataac tgtcagaaag
    15181 ttcgtgccaa tcgaatcata aaaaatgaaa agtgcggata ctgcttgaaa aataccagcc
    15241 atgatccctg ctcttcgatt caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg
    15301 taaaacagcg gaatataagc agcttgttct gtttggaaga gttggaaaaa tttcattagt
    15361 acgacgctta ctgacattgc cgtcacgtag aaaagtacgt attttgttct ttctcctgca
    15421 ttttggaatt gaagtgaatt ataaatcagt acaccgattt gaagcaaaac agctaaaacc
    15481 atggcaatga gtgggaaaat agaagtcgtt tgattcgtca atcctagtaa ttccaatttt
    15541 ttcatcgctg aagcatcaat ttgatttcct tcgcgaacaa taatttctcc ttggaagatc
    15601 atcactggtt gtacagcttc tcgtgcagat tgttttaatt cttctgtctt tttctcattc
    15661 aatacatcat taactacgat gccttgatcc actaagtagc gaatcatttg ttgctgctct
    15721 gttgtgacat tcaaatattg aatctgatct tctgcttctt gtttaaaagc ttccaattcg
    15781 ctttcacgaa cttgtttact catctgttcg tcaatgagct tcaaactttc atccctaact
    15841 gtgtgaattt ccgtttcagt catttcaaat atcgtcgaat aaaaattgtt cggcagtttt
    15901 tggtaaaacg caacgttttc tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc
    15961 cgctcttctg tagtaggttt cgggatattt tcatcttcct tcgccttatc taccttgcca
    16021 ttataagatt tgttgatggt atcattcgtc ttatcgatca attcgaataa ttgcttgatc
    16081 cgattgttct ggtcatctgc cagatctttt tgataagtat attctggagt cactgcttct
    16141 gctgccagtt ttcttctttg ttcggtttct tccgtgttct caatcgtttt attcgcacgg
    16201 atactctctt ctgctacttg gccttctctg aaatcaatgg ccttttgttt gacactacta
    16261 aatgtaacgc cgcagacaat gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt
    16321 ccgagttttt cttgtagttt ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca
    16381 aagcttattc ttgatgaaga tccgcctttt cataagcttt gatgatttcg gcaactacag
    16441 ggtgccgaac aacgtcactt gcttcaaaat taacaaaatc gatttttttg atttgcttta
    16501 atgttcgttc tgcatgaaca agaccacttg tcgtcccacg aggcaagtct atctgacttg
    16561 tatccccatt gacaatcatt tttgattgat aaccgagacg ggtcaagaac attttcatct
    16621 gagcgactgt cgtgttttgt gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc
    16681 cccgcatgta agctaaaggt gcgatttcga tcacgcctcg ttccatcaat cggttcgtat
    16741 gatccaatcc aaaaatttgg tataaagcat cataaacagg ccgcagataa ggatccactt
    16801 tttctttcag atccccgggt agaaagccta ggttttcccc tgcctccaca gcgggccgcg
    16861 tcaaaatgat tttttgcact tcgccttttt ttagggcagc aattgctaat actacggcaa
    16921 gaaacgtttt ccctgttccg gctggcccta caccaaatac gatgtcatgt ttggctaccg
    16981 attcaacgta tttcttttga ccgctgtttt ttacccgtat aggttttcca ttacggtctt
    17041 tgacgatctc ttcctcatac atctcaacaa aataatctaa tgtacctttt tgccccattt
    17101 tcaatgccgt cacaacatct ggcgtactta cgtggattcc tcgtttgatc aattcctgta
    17161 atgtccgaat gactgaggca gccaaggaaa tctgggattg ctcaccaatg atctgaatga
    17221 tttctccacg tgtatggatg gttgtttccg ttgtttcctc gatcagtttt atatgcttgt
    17281 cgtgtgcacc taaaagcatg ctgacatcat cagcagctgt caatctaatc tctaaagaac
    17341 tagactcttc cgtcaaaaat agacatcccc tttttttcga ttcattcttt cctattttac
    17401 catactcaga agaaactata tagcaaataa gaaaaggttg taaaagagtc gcttaaccat
    17461 acgtaccaag aaacgttaaa aaatagccgt ttccattcgc taaaggtctc gttttgataa
    17521 aaagaagctg agaatgtaag ttatatgaaa gtggagaaca aaatatttcg tttcattcaa
    17581 caagtattct ctaggtattt ttcctgtttc aaaaaaagaa aacaaaatag ttaaaatatt
    17641 cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca aataaacata cagcaaagcg
    17701 tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt gagtcatgta agtgagaaat
    17761 gacaataaat cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg
    17821 tggctgcctc cattatcagg ctagcggata actttatcaa aaagcgtccg tgaatgaaga
    17881 agctttttta agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg
    17941 gaaaagggct tttttttatt ttaaggtggt ggttttcatg gaatctgata ttagtggcat
    18001 tactaattgc tgcaacgttt atatcgtcaa tcaaaatgat aattattgga ggaaaaattt
    18061 atggaagaaa caaaaactta ttcatggaaa caactattga tggtcagttc acttatcttc
    18121 gggatgttct tcgggtcagg taatctaatc tttcctgtcc acttaggtca aatggctggc
    18181 gcaaactggg tcagtgctgg cgtaggattt gctatctcag gtgctctttt cccattacta
    18241 gctatcttag ctgtagtagt gacaaaaagt gatggtctgt atgacttagc taaacctgtc
    18301 ggaaaatggt atgctgcctt gttcttaatc cttgttcatt tgacgatcgg accatttttc
    18361 ggtacaccac ggacagctgc tactgctttt gaaatggctg ctaaaccatt tttgcctgaa
    18421 aaatataatt cattaggtat gtttttattc tctgctcttt tctttttact tgcttatttc
    18481 cttactgtcc atccaacacg gttggtcaaa tacgtcggaa agtatctaaa ctccttgttt
    18541 cttgcattat tagcaatcat ttttgtcgtt tctttattcc atccaatggg aaatctaaat
    18601 caatctgctg cagatactta tcaaacaaac gctttaacaa atggtgtcct agaaggttat
    18661 aacacggttg atgcagtagc tttgcttgcc ttaagtgtca cttttgtcca tgcagtccgt
    18721 gaccttggtt tcaaagataa aaaagtcaca gaattaacgg caaaagctgg tagcctgagc
    18781 gttgcgctag aagtcatgat ctattttgga cttgttcttc tcggtgcgat gagtttgaat
    18841 aagatgtctt tatcagaaaa tggcggaact gcactttctc agatcactaa tttttattta
    18901 ggtaacttcg gcgctagctt cttaggagtg atggtcacat taggtgtctt cactaccgct
    18961 atgggactcg ttgtttcttt tgcgcaagat ttccataaac tgttccctaa agtcagttat
    19021 atgacatggc tgcggttaac gactttcgtc tcttttgtcg ttgcaaatgc tggtttagac
    19081 aatatcatcc aatggtcttt accagtattg atgcttttgt atccattgtc gcttgcgttg
    19141 atcttgcttt ccttaacagc aaaatttttc caaaagacac catttgtcta tcaagtaacg
    19201 atgttgtttg ctgctgttcc tgccgtattg gatatgctgg caaattcgcc tgctttagtg
    19261 agtcaacaaa gagttgtagc aagcatgctc gaattttatc atcatcacgt tccttttgct
    19321 gcattaggac ttggctggat ggtcccaacg ttacttggtt acgctggcag cctactgttt
    19381 tattacgcct atcgcctatc agggtataaa caagaagcaa atgaactacc agaagaataa
    19441 ccaaatcaaa cacaatttaa cataagaaat attaaacagg aaaaaaccag ctagatgaaa
    19501 gaatcctatt ctttcgtcta gctatttttt agttctcttt tttacgcgca caaaaaaact
    19561 gcggacaatg attgtccgca gtttaaatgg gctctataat atctagtgtt ggtgacgcct
    19621 tctgttgcca atactttttc ttttttctaa taaacgcaaa cagacacctt gaaatccttt
    19681 agaataaagt cgacgaaaac cattcaaagg agtgtttcaa gatgtcctat tcagagttta
    19741 ccaaagagtt actagatatt ctagacctaa atcttatctt tcatgaagat gcctttcgca
    19801 aagagcgaat caatgacgaa acatgttttg tttttgatgg cacattgacc tatcaaccag
    19861 aggaatgctt ccattgtcac tatcaaaata agcaaacgat cattaaatgg ggctggaaaa
    19921 aagtatccat cttattgaat gatgtcagta actacaagac cattctgcgg attaacaaac
    19981 agcgcttcaa atgtaaacac tgtggaaaga cttttttagc agaagattca gttagtgatc
    20041 gtcgctgctc cattgctcgc agagtcaaac aagccattct tgagctatta agcgaacctc
    20101 tttctatgtc tttgatcgca cgtatgaagc acatttctcc gaccactgtc attcgaatac
    20161 ttcgcagcct tcggcctaaa accgtttctt tgaaccagcc gttaccagaa gtggtgtgtt
    20221 tcgatgaatt caaatcgg
//
LOCUS       DADQRX010000051        19883 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.052, whole genome shotgun sequence.
ACCESSION   DADQRX010000051 DADQRX010000000
VERSION     DADQRX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.052"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..605
                     /locus_tag="J8V14_001701"
     CDS             123..605
                     /locus_tag="J8V14_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921807.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            802..1692
                     /gene="pstS"
                     /locus_tag="J8V14_001702"
     CDS             802..1692
                     /gene="pstS"
                     /locus_tag="J8V14_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBA0921808.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2017..4488
                     /locus_tag="J8V14_001703"
     CDS             2017..4488
                     /locus_tag="J8V14_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBA0921809.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4591..6327)
                     /locus_tag="J8V14_001704"
     CDS             complement(4591..6327)
                     /locus_tag="J8V14_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBA0921810.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6324..7028)
                     /locus_tag="J8V14_001705"
     CDS             complement(6324..7028)
                     /locus_tag="J8V14_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0921811.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7159..7662
                     /locus_tag="J8V14_001706"
     CDS             7159..7662
                     /locus_tag="J8V14_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBA0921812.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7622..7960
                     /locus_tag="J8V14_001707"
     CDS             7622..7960
                     /locus_tag="J8V14_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBA0921813.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7981..9399
                     /gene="ffh"
                     /locus_tag="J8V14_001708"
     CDS             7981..9399
                     /gene="ffh"
                     /locus_tag="J8V14_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBA0921814.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9511..10089
                     /locus_tag="J8V14_001709"
     CDS             9511..10089
                     /locus_tag="J8V14_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBA0921815.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10093..10614
                     /locus_tag="J8V14_001710"
     CDS             10093..10614
                     /locus_tag="J8V14_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBA0921816.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10693..11247)
                     /locus_tag="J8V14_001711"
     CDS             complement(10693..11247)
                     /locus_tag="J8V14_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBA0921817.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11500..11775
                     /gene="rpsP"
                     /locus_tag="J8V14_001712"
     CDS             11500..11775
                     /gene="rpsP"
                     /locus_tag="J8V14_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBA0921818.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11787..12038
                     /locus_tag="J8V14_001713"
     CDS             11787..12038
                     /locus_tag="J8V14_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBA0921819.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12163..12954
                     /locus_tag="J8V14_001714"
     CDS             12163..12954
                     /locus_tag="J8V14_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921820.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13124..13645
                     /gene="rimM"
                     /locus_tag="J8V14_001715"
     CDS             13124..13645
                     /gene="rimM"
                     /locus_tag="J8V14_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBA0921821.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13635..14396
                     /gene="trmD"
                     /locus_tag="J8V14_001716"
     CDS             13635..14396
                     /gene="trmD"
                     /locus_tag="J8V14_001716"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBA0921822.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14532..14879
                     /gene="rplS"
                     /locus_tag="J8V14_001717"
     CDS             14532..14879
                     /gene="rplS"
                     /locus_tag="J8V14_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBA0921823.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15011..16147)
                     /locus_tag="J8V14_001718"
     CDS             complement(15011..16147)
                     /locus_tag="J8V14_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0921824.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16263..16829)
                     /locus_tag="J8V14_001719"
     CDS             complement(16263..16829)
                     /locus_tag="J8V14_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921825.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(16965..17618)
                     /locus_tag="J8V14_001720"
     CDS             complement(16965..17618)
                     /locus_tag="J8V14_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921826.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(17668..18096)
                     /locus_tag="J8V14_001721"
     CDS             complement(17668..18096)
                     /locus_tag="J8V14_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBA0921827.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18101..18475)
                     /locus_tag="J8V14_001722"
     CDS             complement(18101..18475)
                     /locus_tag="J8V14_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921828.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            18779..18964
                     /locus_tag="J8V14_001723"
     CDS             18779..18964
                     /locus_tag="J8V14_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0921829.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            18977..19225
                     /locus_tag="J8V14_001724"
     CDS             18977..19225
                     /locus_tag="J8V14_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921830.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(19528..>19883)
                     /locus_tag="J8V14_001725"
     CDS             complement(19528..>19883)
                     /locus_tag="J8V14_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921831.1"
                     /translation="TLSYFLVKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKIN
                     CSHYHANGFDMGSIHKSSLLSPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRT
                     ANYQKKFKLKLKDSF"
BASE COUNT         6396 a         3563 c         3920 g         6004 t
ORIGIN      
        1 ttctagatta aatgaataaa ctctcatgcc attatctcta aaaaaaaagt ttttccccct
       61 aattttttta gatagaaaat tgtatagtat gatgagagaa aaagtagttt gggggaacgt
      121 taatgagaag aaaagcagta atcgatcggc tttatttatt tttttggttt tttcctatcg
      181 ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg tgcggagata ttaggcctga
      241 tccaaggaag cggatttgtg gtttctagga attttccagc tttgactgtt ttctatttag
      301 tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc
      361 tttacttttc ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta
      421 tcggccctaa tggtttgagc gagagtggat tgaaggattc agcttattcc tccatgtatt
      481 tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt
      541 tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact
      601 attgatggtg gatcatcagt aaaaaagcat atgagctgcg taaaatgcat gtttctcata
      661 tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt
      721 aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt
      781 aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt
      841 cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat
      901 tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg
      961 ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta
     1021 caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga
     1081 aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat
     1141 aaagtagcag tggtcgggat ggcgccaatc gtcaataaag atacagatgt caaagatatc
     1201 accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc
     1261 gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca
     1321 ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca
     1381 ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca
     1441 tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat
     1501 gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct
     1561 tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct
     1621 gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga
     1681 aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca
     1741 actatgtttc ccacatatac atttttcctt taattgtgaa aaatagttcc tcatattttg
     1801 ccgttttttg agctaatacc gagggtcagc tgattaaaca ccgtttattc gaaaaaaaga
     1861 gctacgacat agaactgtcg tagctctttt tttgctatat gacaggaatg aaaggtgttt
     1921 gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg agaagtttgg
     1981 aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg gataaatcga
     2041 agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct ttttccatcg
     2101 tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat
     2161 aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa catatttacg
     2221 tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga atcctataag
     2281 aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt tcaggaaatt
     2341 cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt caatgtcata
     2401 cgggacggga tagaacaaac aatagatata gaagaccttg taatagatga cattgttatt
     2461 ttagagactg gacatcaaat tccagcagat gcagaggtcg tggaaggaaa actgcaagtt
     2521 aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg cgatccatta
     2581 atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa agttggagca
     2641 gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg agaacagtcg
     2701 gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat tattcctatc
     2761 ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa agaaagtgtc
     2821 gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt gctgacgacg
     2881 attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt acacaatatg
     2941 aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac aggaacgatc
     3001 acagaaaata aaatgtctgt tcaaaaagtg atcgtctcta aaaaacaaga aataatcagt
     3061 cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc aggagacaat
     3121 gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc ctatcaccat
     3181 gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga caaggtgtat
     3241 gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc ggaagagttt
     3301 gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt
     3361 gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat tgctaatcct
     3421 atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt agccatcaaa
     3481 gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc agggatttca
     3541 catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga agcagctgtc
     3601 gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg
     3661 ataaagcgta acaatacagt agctatgaca ggtgatggcg tgaatgatat tctagccatg
     3721 aaagaagcag actgcagtat agcgatggct tccggaaatg aagccgccat gcaggcctca
     3781 caagtggtct tacttgattc tgacttttca cgcatgccag aagttgttgc agaaggacgg
     3841 cgcgttgtga acaatattga gcgatcagcc agcttgtttt tagtgaagaa tattttctcc
     3901 tttttgctct cactgttttc agtgatcttt gctctgacct atcctttaga accatcacaa
     3961 atcacattga ttagtttgtt tactatcgga cttccatctt tcttgcttgc tctagaagaa
     4021 aacaaaaagc gaatcagagg gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt
     4081 ggactaacag atatgatcgt agtcggtgcg ttggtgatct gcggggtgac tttgaatcta
     4141 aataaaacag atgtttctac agcatctacc atgcttttga ttgcagtagg gttcctcgtt
     4201 ctctataaaa tctgttcacc gctgaataaa ttcagaagtc agatcattct tttctgtgct
     4261 agcggtaact ttttttcggt catctttttg cataagctgt tttcaatcac cggtatctca
     4321 gctgtatcac ttttgctgtt gagcattcta tttttctcgg cagattcgat ttttaggcat
     4381 ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt
     4441 agtcgcgtag ctaaaaaatt tctatcgata tttaaacgag aaaattgacg aaaagtatct
     4501 caaaaaagag taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca
     4561 actttaaaac gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt
     4621 cgaacctgtt ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc
     4681 ttttacaatc gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac
     4741 gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc
     4801 ttggacagaa aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct
     4861 ttttgaatat tggacagcat tttcaatcag atttttgata attggataaa ggatatctgt
     4921 tttagtagta aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat
     4981 gattggctga taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat
     5041 ctcgtaagaa ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa
     5101 ccggatcgca tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt
     5161 tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc
     5221 gtgagagacg ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa
     5281 gtcatacgcc acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact
     5341 caagtctaat gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc
     5401 aggagtcact tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg
     5461 ctgttcaggt tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag
     5521 taaacgagaa tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt
     5581 atataattgc tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa
     5641 cgcattaatc gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc
     5701 ctgattagga ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt
     5761 tttttgatag atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa
     5821 gaaaaagacc caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc
     5881 gatacgtaaa atggcttcta actggccact ttttttgact ggtaatgcga catataaaag
     5941 atcctcattc aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac
     6001 ggctttgatt tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa
     6061 taagatcttt ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc
     6121 ttccaaagca gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag
     6181 cagttgtgct ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata
     6241 atgggaaatc aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag
     6301 acgttcataa atttttttcc atttcatcgt tttggttcct gaaacttata gccaaatcct
     6361 ctaatagtca ccaagtactt tggatgtttt ggatctcgct cgattttttc tcgcaaatga
     6421 ctaacgtgta catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct
     6481 agcaatgtgt ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca
     6541 aattctttcg gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa
     6601 tcagcagtga tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta
     6661 cgaggttcaa tccgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat
     6721 ggtttggtca aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca
     6781 tctttagccg tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg
     6841 atctccatgc catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt
     6901 tctaatgcta attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct
     6961 ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt
     7021 tttttcattc aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca
     7081 gtacaaaggg ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat
     7141 agaaaggaag agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc
     7201 tatgatggac agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct
     7261 gtcatggtct gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc
     7321 attggtggaa atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc
     7381 gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa
     7441 aaacacattg atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt
     7501 tatgacggaa gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt
     7561 attcagcaaa aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag
     7621 aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt
     7681 gacagaaaaa caaatgaact acatggaatt atattatgca gatgattttt cattaggtga
     7741 gatcgcggaa gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag
     7801 caagatcttg gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga
     7861 acaggtattg gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact
     7921 acttgaatat gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat
     7981 atggcatttg aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa
     8041 ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta
     8101 gaagccgatg ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg
     8161 gtaggggcag aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat
     8221 gaagaattga cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata
     8281 ccaactgtta tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa
     8341 ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta
     8401 tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta
     8461 tttgatatgg gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca
     8521 aaagaaaaga aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa
     8581 acattgatgg atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt
     8641 gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa
     8701 ttaggtatta caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca
     8761 ctatcgatcc gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg
     8821 actgacttag aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat
     8881 atgctgacat tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt
     8941 gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa
     9001 gtgatgggta tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc
     9061 cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt
     9121 tattctatga cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc
     9181 cgaattgctg ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc
     9241 aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg
     9301 gaccaaatgt ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg
     9361 atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt
     9421 acagactgaa aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat
     9481 acaattactc taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa
     9541 agtatgcggc aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg
     9601 ttccgaaaat tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat
     9661 aaaagtgaag cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat
     9721 ggagaaacaa aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa
     9781 atcgaagcag ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt
     9841 ttttcagagt atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca
     9901 caagaagaag tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa
     9961 agaggattta aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc
    10021 atcaatgatc accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag
    10081 ggcgaataaa gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg
    10141 gcaacgaaaa cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact
    10201 tatgaagctt tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa
    10261 gcaaaaaaat atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc
    10321 acccacgcac aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt
    10381 ctaaaaaatc tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg
    10441 atcaaaccgc tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta
    10501 ttattagggg acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg
    10561 ctagacacga cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa
    10621 ataaaataaa aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta
    10681 ccgttatgct ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt
    10741 ttggaagaaa cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac
    10801 gtttccgata tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg
    10861 gcgtagatgg tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa
    10921 acctggttta tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt
    10981 tttcaatact gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag
    11041 tcccctcact tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag
    11101 atcctcattg taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg
    11161 aagcaatttc gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc
    11221 aataaaaaat ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa
    11281 tcttacaaaa gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga
    11341 agatagatta cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa
    11401 tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac
    11461 tacatcttgt gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta
    11521 aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct
    11581 cctcgtgatg gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca
    11641 gaagtagttt taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct
    11701 gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa
    11761 ttctcaaaga aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg
    11821 ttcgtccgct agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact
    11881 tttacgagta caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag
    11941 ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa
    12001 aagtgcgttt gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt
    12061 ttcgagtgca gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa
    12121 gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta
    12181 tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat
    12241 acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa
    12301 acggcttcca ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta
    12361 gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca
    12421 ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga
    12481 acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc
    12541 tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt
    12601 gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa
    12661 ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt
    12721 gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg
    12781 gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat
    12841 acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact
    12901 catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag
    12961 cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg
    13021 ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac
    13081 acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa
    13141 tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac
    13201 ggatttccca gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa
    13261 agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag
    13321 ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt
    13381 gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg
    13441 tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg
    13501 tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat
    13561 cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga
    13621 aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc
    13681 ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct
    13741 ctaacttccg cgattattca gataacaaac atcagacagt agatgattat ccatacggcg
    13801 gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa
    13861 aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg
    13921 atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt
    13981 atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt
    14041 atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt
    14101 tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat
    14161 tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat
    14221 tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga
    14281 cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat
    14341 ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt
    14401 ttctaaaatc ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa
    14461 gcacttaact attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg
    14521 agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca
    14581 tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc
    14641 gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg
    14701 aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc
    14761 atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat
    14821 tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat
    14881 catcgttatc agacagagaa agaacccttg gtattcaagg gttctttttt tattatctgt
    14941 ttttttatat taacgatttc tatttggtac atttttgatt aataaaaagg ggcaaaaaag
    15001 gggcaacaag ttacagtttt tctatatctt ctttcaattt ttcttttact ccttttgtca
    15061 catgcaaata aattttttca gtgatatcac tattttcgtg accaacgcga tcctgaatgg
    15121 catacagagg agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg
    15181 aactaagttt tttatcaatc ttcatgtcag ctttatgact tctcaaatag gtgttaattg
    15241 ctgttagttg gaaaggagtt cctttcgttg tttgaaatag aaattgacca tttggattta
    15301 attctagaag ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc
    15361 cggcagctgt ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca
    15421 tcatcgtccc atttataact actgatgcat tattattggt aatgtgtaca tcatctttac
    15481 ttaatgcgat tgcttcacca gacctcatcc cagtcaaata tagccactgg aaaaggagag
    15541 aatatctttt attgtgtgaa gttgtgaaat ctactagtct attatattca tcatcttcta
    15601 aaaatttatc tttaattttt atcgtttttg actctctttt ataatcaata actacttcat
    15661 cgataggatt tttttctaca taaccttttt tcatagcata tgaaaaaaga agattcaatt
    15721 tggatttaat tacactgacg tacttatttg acaagtcatc tttatatatc atgtcttcaa
    15781 aaatattatt caaatctatt gtattaatac cagaaacgat gtaagtctca ggtatcttct
    15841 tctttatagt gtttaatata ttattcgtag gataatatgt agattcctta acttgtcttt
    15901 tgtatataac taaccattct tcaacaagct catgaaatgt tagatcaggc ttatgaagtg
    15961 ttttttcgtt taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag
    16021 tttctcgaga tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat
    16081 caatatatcg ctcattatat ttatatactt tttttccgtt tttatcagtt ttagtttcga
    16141 tccacattgt tttcatctcc tatttgttgc tataatagga atagataagt aagccgatta
    16201 tagcaggttt atttttcatc acgtccacaa actttggcga gggaggggcg tgtttttgtt
    16261 agttattgtg gtaggttatc taaagcgtat tgtgcttgct catttgtaaa accttcaaat
    16321 ataagttgat cgtacaattg cgaatctgac attgatgccc aatcatcgta actttccgct
    16381 ttagccaaag cttgttcgtt gtaatcgaca tcaacattag ccagcgcata atcgattgca
    16441 tcacttggat attcttcaaa ctctagttgt tcacgcaaac cttgttcaga cataccagcc
    16501 caacctagat agctttcagc tttgctcaat gcatttctgt attcacgagg aacactttct
    16561 tcttgagtaa cttcttctat agtagtttca gtagtttcag tttcttcttc aacgactgta
    16621 gattctgcta ccgattctgc agaagacact tctgtcacgc tttctttagt tgtttcagcg
    16681 acggtatttt tagcatcatc actgcctcct tgcgaaccta tagcaataaa caccacaaca
    16741 accgctaaca accaaaacca tacccgttta taaaacggtt tcttaacttt atacattttc
    16801 ccatcttgac ccataacttt ttttgccatt taaatatccc tcgcttcttg ttataatata
    16861 tttgtgatct cagaaatgag gtatgagtcc gtgttgcagc acgggctttt ttctttataa
    16921 cttttttaga gattataggc aaaatagtag ggcataaaaa tgtattattg aattccgtat
    16981 ttagaaaatc ctaattgaac ttcaccggaa gtcttttgct gtgttgtacg caatgcttct
    17041 tcttcagaca ttccattctg tactttccat gcaacaggcg acatcccgta tttgttaaca
    17101 aaatcagtaa gtgataaagt gtcagcgtct tgctgagcgc ttgtttgttg gtcttctgga
    17161 ttctgttgag atgctgcttg ttgttctttc tgatcttgac tgataatatt gccagcatca
    17221 tctgtagtca atccattttc ataaagggcc acgccgaaag cttcccactc tttgttggac
    17281 caatttgcac gatcagctgg agttgactgt aaagtgcgtt gtttcatctg ttcatatgtt
    17341 tcttcttgag gtgcggtttg gattgtactc tgactggagc ttataactgt tgggctaggt
    17401 tccgccgtag cttggttgga gcttgtaact gttgaactag aatctgtttt agatgtagat
    17461 ttgctagtag atgaactggt ttcagttgtt tcttttgttt tacttatttt tgtttcttgg
    17521 ttagaagtgg tatctgttga ttcagctttt ttattatttg aacaagctga aagtagcaga
    17581 gcagtactta acaacaacat aacgctaact tttttcattt tataattcct ctttctcgtt
    17641 gtaatatgtg tgctaacacg ggctttttta tataagaaaa cgataagcgc tttctggaag
    17701 cccgtaaaga ttctttaatt cctcgatttt tttaggatat tgatcattat cttctttata
    17761 aagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt tgcttctaga
    17821 tgttcttgtt gatacataat agctggataa gccacgatga aaaatagcgt gacctaattc
    17881 gtgagcgcaa atgtagaatc tttcctcaga gtctcgcagt tcatcattta aaaagattat
    17941 cgcacgatct ctaatttctt gaaactgccc cttgggattc tcgataaaag gaacgtattg
    18001 aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt atttccgctt
    18061 caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt ttgccttttt
    18121 ctttgtcttc tttcacaata tcccagaaag tcgctatcag gatatctttt acgcgctgta
    18181 tttgttcggg tgtcaatgtt tccccaccat aagacatatt aacatttgag tctagtagtt
    18241 tatcaagttc aaccacttcc tcttttgtag cccacttggg aacattatta tttcccaata
    18301 aataatcagt tgtgacacca aaataatcag caactttctt taagttctca gattttggcg
    18361 aggctttatc ccatcttctt atttgtccat tagagatgcc cacctgtctt tctacttctg
    18421 ctatagtcac atgcttttcg tctgctaatt ctttaatctt agtaactaaa ctcattatta
    18481 tcaacctttc aaagctgaaa agaaaataaa tagcttaaaa gttatatttt tggttgacaa
    18541 ttagctttta agctagtata tttaattcgt aagctaaatt gttagctaaa taagagcaac
    18601 aaaaaactct actaatttaa aacattctct cggtcgccaa acttagaaat gttattttag
    18661 aggcttttta taagtcttat ttaactatgt attcataata gcttaaaagc taataggtgt
    18721 caacgattta gctaattttt tagcttacaa attatttgtt tagaaaggag ctatttttat
    18781 gtctgagaat ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa
    18841 agaactagct gcgcttatcg gtatttcagg agcttattta tcagatattc taaacggcaa
    18901 tcgtgatgga aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg
    18961 ataggggggg taggcgatga caaaactaaa aaaacaagat tttgtaaaaa aatataatta
    19021 ttctccatct acttatcaac gtcgaatgtc ggaactaaaa aatacagcaa ttttctcagc
    19081 ggcgtatgaa cgggtcacag gacaagaagt ttggatcaat acagaattat acgataaatt
    19141 tttgtctttc aaatcctata acaggttacg cacaagaaag gtaacaccta aagaatttat
    19201 cgagaagcat ttagttgatt tataaaaaac agaacatttt gagaggtgaa ggttaatggg
    19261 taaattcaac agagcattag tattcagcgc accgctaatc gtctatgctt tagggctttg
    19321 gggaagtaga caagcgttga taggaacgat tgtgtacatg gtttggattt ttatagggct
    19381 ggatgaagct gagtacaaaa caaaaaagcc agtcgggagg gactgactaa tgagtaagga
    19441 cgatattgta tttctgttaa ttatgttttg tgggattgga tcaataataa ttaatttgta
    19501 tttgattatt aaagaaaaat ttttcaatta aaatgagtct tttagcttta acttaaattt
    19561 tttttgataa ttagctgttc ttatgaccat tacaagtttt tcaccaggta atatatcttt
    19621 catatctaaa acttctgaaa aagcgactga gtagccagga gagattgctt gtgccaacgg
    19681 tataggagat aaaaggctac ttttatgaat gcttcccata tcaaaaccat tggcgtgata
    19741 atgtgaacaa ttaattttct taccgttgta aagatacgta cattcgataa ttgagtttgg
    19801 catttttgat gggtttgcaa ttacgccagt aagaatcatt cgattacttc tatcatgctt
    19861 aactaaaaaa taggagagcg taa
//
LOCUS       DADQRX010000052        19694 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.053, whole genome shotgun sequence.
ACCESSION   DADQRX010000052 DADQRX010000000
VERSION     DADQRX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.053"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(216..446)
                     /locus_tag="J8V14_001726"
     CDS             complement(216..446)
                     /locus_tag="J8V14_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921832.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="J8V14_001727"
     CDS             1063..1725
                     /locus_tag="J8V14_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921833.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="J8V14_001728"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="J8V14_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBA0921834.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="J8V14_001729"
     CDS             5145..5459
                     /locus_tag="J8V14_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBA0921835.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="J8V14_001730"
     CDS             5472..5846
                     /locus_tag="J8V14_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBA0921836.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="J8V14_001731"
     CDS             5856..6200
                     /locus_tag="J8V14_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBA0921837.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="J8V14_001732"
     CDS             6271..7623
                     /locus_tag="J8V14_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBA0921838.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="J8V14_001733"
     CDS             7683..8825
                     /locus_tag="J8V14_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBA0921839.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="J8V14_001734"
     CDS             8850..9104
                     /locus_tag="J8V14_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921840.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="J8V14_001735"
     CDS             9360..10544
                     /locus_tag="J8V14_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBA0921841.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10559..10678
                     /locus_tag="J8V14_001736"
     CDS             10559..10678
                     /locus_tag="J8V14_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBA0921842.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            10683..10904
                     /locus_tag="J8V14_001737"
     CDS             10683..10904
                     /locus_tag="J8V14_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921843.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="J8V14_001738"
     CDS             10917..11417
                     /locus_tag="J8V14_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBA0921844.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="J8V14_001739"
     CDS             11514..13361
                     /locus_tag="J8V14_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBA0921845.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="J8V14_001740"
     CDS             13364..14260
                     /locus_tag="J8V14_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0921846.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="J8V14_001741"
     CDS             14310..14699
                     /locus_tag="J8V14_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0921847.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="J8V14_001742"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="J8V14_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="J8V14_001743"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="J8V14_001743"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBA0921848.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19694
                     /locus_tag="J8V14_001744"
     CDS             18893..>19694
                     /locus_tag="J8V14_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0921849.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DADQRX010000053        19078 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.054, whole genome shotgun sequence.
ACCESSION   DADQRX010000053 DADQRX010000000
VERSION     DADQRX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.054"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..245
                     /locus_tag="J8V14_001745"
     CDS             6..245
                     /locus_tag="J8V14_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921850.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="J8V14_001746"
     CDS             312..521
                     /locus_tag="J8V14_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921851.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="J8V14_001747"
     CDS             581..1252
                     /locus_tag="J8V14_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBA0921852.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="J8V14_001748"
     CDS             1305..3281
                     /locus_tag="J8V14_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBA0921853.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="J8V14_001749"
     CDS             3294..4049
                     /locus_tag="J8V14_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBA0921854.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="J8V14_001750"
     CDS             4046..4870
                     /locus_tag="J8V14_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921855.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5159..7270
                     /locus_tag="J8V14_001751"
     CDS             5159..7270
                     /locus_tag="J8V14_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBA0921856.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="J8V14_001752"
     CDS             complement(7308..9365)
                     /locus_tag="J8V14_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBA0921857.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="J8V14_001753"
     CDS             complement(9479..9691)
                     /locus_tag="J8V14_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921858.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="J8V14_001754"
     CDS             9878..10345
                     /locus_tag="J8V14_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921859.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="J8V14_001755"
     CDS             10342..10584
                     /locus_tag="J8V14_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921860.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="J8V14_001756"
     CDS             10602..10904
                     /locus_tag="J8V14_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921861.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="J8V14_001757"
     CDS             10976..13030
                     /locus_tag="J8V14_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921862.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="J8V14_001758"
     CDS             13030..15642
                     /locus_tag="J8V14_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBA0921863.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="J8V14_001759"
     CDS             15639..18170
                     /locus_tag="J8V14_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921864.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="J8V14_001760"
     CDS             18222..18554
                     /locus_tag="J8V14_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921865.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..>19078
                     /locus_tag="J8V14_001761"
     CDS             18555..>19078
                     /locus_tag="J8V14_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921866.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         6854 a         2966 c         3803 g         5455 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagg
//
LOCUS       DADQRX010000054        18739 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.055, whole genome shotgun sequence.
ACCESSION   DADQRX010000054 DADQRX010000000
VERSION     DADQRX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.055"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(125..1036)
                     /locus_tag="J8V14_001762"
     CDS             complement(125..1036)
                     /locus_tag="J8V14_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0921867.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1413..2051)
                     /locus_tag="J8V14_001763"
     CDS             complement(1413..2051)
                     /locus_tag="J8V14_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921868.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2055..2825)
                     /locus_tag="J8V14_001764"
     CDS             complement(2055..2825)
                     /locus_tag="J8V14_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBA0921869.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3243..3659)
                     /locus_tag="J8V14_001765"
     CDS             complement(3243..3659)
                     /locus_tag="J8V14_001765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921870.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3710..4091)
                     /locus_tag="J8V14_001766"
                     /pseudo
     CDS             complement(3710..4091)
                     /locus_tag="J8V14_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4217..4882
                     /locus_tag="J8V14_001767"
     CDS             4217..4882
                     /locus_tag="J8V14_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0921871.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5279..7312)
                     /gene="brxL"
                     /locus_tag="J8V14_001768"
     CDS             complement(5279..7312)
                     /gene="brxL"
                     /locus_tag="J8V14_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HBA0921872.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7430..9952)
                     /gene="pglZ"
                     /locus_tag="J8V14_001769"
     CDS             complement(7430..9952)
                     /gene="pglZ"
                     /locus_tag="J8V14_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HBA0921873.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10068..13700)
                     /gene="pglX"
                     /locus_tag="J8V14_001770"
     CDS             complement(10068..13700)
                     /gene="pglX"
                     /locus_tag="J8V14_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HBA0921874.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13764..17435)
                     /gene="brxC"
                     /locus_tag="J8V14_001771"
     CDS             complement(13764..17435)
                     /gene="brxC"
                     /locus_tag="J8V14_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HBA0921875.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17460..18035)
                     /locus_tag="J8V14_001772"
     CDS             complement(17460..18035)
                     /locus_tag="J8V14_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HBA0921876.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18035..18634)
                     /locus_tag="J8V14_001773"
     CDS             complement(18035..18634)
                     /locus_tag="J8V14_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBA0921877.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5788 a         3568 c         2620 g         6763 t
ORIGIN      
        1 aatttttaaa ataaaaagaa ctaaatcaag taataattaa actactgttg atttagttct
       61 aataaaaaga tttagatgaa cagttttatt gatttaaata gttcatattt tttatttata
      121 ccgcttattt actacttgct gtactagaat ctgaagtgaa tgtactcaaa acattttcaa
      181 agtctttgtc tttgattttt acattagcgt ctttcaatac ttcgccaatt actttattct
      241 ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc tttcaattct tttttgtatt
      301 tatccatgtc gttgcctttg ttttggtttt tcaccatttt aaccacataa tattccgtag
      361 tataagttga agcattggta gaagtgatca catcagaaac ttgaccatct ttcaacttaa
      421 aggctgcttc tttgacttct gccggaaccg ttgtagtagt ggaatcaaat ttcacttttc
      481 cgccatcttc ttttgtcgta gtatcagttg acttgtcttt ggctaattta gagaaatcgt
      541 ctcccttatc agcagctttc ttcacatctt ttgcatcatc ttcacttgct acttggataa
      601 tttgagcttc cacttctgga tggaaagatt cccaagccgt ttttaaatct tcatcggtca
      661 atttgatatg tttctttaga ccttcttgaa aggctagact ttgtttgata ctgtctttga
      721 atgtttgttc tgtgtaacct gcagatttca attgtgaatc aaatgaatca cccaattgtt
      781 tctttgtttg atcatattgg gaatcgattt ctttggttga tacgtcatct ccatatttat
      841 cttcaaatac ttgataaacg atcaactgtt gcacagcgga atctgctcct tgcatttttt
      901 tcaagctatc cattacttct tgttcgctga ttgtaccgcc tttataacta acggctgttt
      961 tttcaccggc attcgtacag cctgacaata agccagtgcc tagtacgatg gcaagtgcta
     1021 aaaatctttt tttcatttat caattctcct acttttcgtt ttttgcgaat tgctaaaaac
     1081 aaaggtgttc aagatgaaaa tcatcaagaa cacctttact aatctgctcg atacgtcctg
     1141 aatagatata aggctacaca atccagaaag ttcgctcagc agaataaatg tcaagtagca
     1201 catcacacct taatatacta cttcttttgt taccgttttt caagtctatc ttgcgttccg
     1261 tatttaacgt aactaattct tagaacaaat gttatcgatt ttgtttttct tcttccgctg
     1321 ttgggatatt caaatcagac acttttatgt tcgattttcc agacattttt ttgttgtttt
     1381 tattagaaaa agtttgtttt ttcatttgtt ttctaaagca tgatcttctt gtttcctatt
     1441 ttggtcagat atggatcatg cgtgaccacg ataactaacg taccttggga agcctgttgc
     1501 gataagattt caacgacttg ctccttattt cgtttatcta atgatccagt tggttcatcg
     1561 gctaaaatga ttttgctatt cttcaataag actctcgcac aggcgattct ttgcttctca
     1621 ccaccagata atgtgtagat aggcgtactt agcttcttgt ttatatttac ttttttcaat
     1681 gcctcatact ttgctgttcg cttatctgaa acatttaaat tcgaaaactt caatccaata
     1741 tctaaattaa aatcgattgt ttcatattca atcaacccaa aatcttgaaa aagaaaagag
     1801 agggcatttc tttttatcaa gcgcttctct cttccagtta aatgttttgt ttcttgatta
     1861 aataaataaa attgccctga actgaattga tcattcaatc ctaatatatt caataaagta
     1921 cttttccctg ctccgctctc tccaactaca gtcacaaaat ccccagcatc cgcagtgaaa
     1981 cgatccaaat gaagaatcgt tttgtttcga atgatttttt tcgcaccttt tatcactatc
     2041 aacggctcca attgttaacc tccattcatc agcaataact gacttttctt ttctgtagac
     2101 aaaataaaaa caactgaaag aataaaatca atgaagctaa agaaaaaaat attaaaaaga
     2161 tatttaggct ctctttctat aagaaaaagg aagattacac ttgtcgttcc ccatttgatg
     2221 aggttcatca tgaagaaatc tttatacgtt tcaaagagcg aataaccact tgaacgaaag
     2281 atagcgattt tccgacaatt gtagacaaaa tatatgcata acatgacata gctcaacaag
     2341 aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt aatgaaatga tctgcttaac
     2401 actttgacag ctatctcttc agtctgagta tacggaaatg ataactgcaa gttatcacgt
     2461 aactgacttt cttcaatcag ctcaaataac ctttctttcg acaaagagcc tatcttgatc
     2521 tttaacggtg gatagatttc tccgctgaga atatttcttt cccaatacgt actgtttttc
     2581 aatgtcagta tttcaacaac cggataatgt tcgatccagg ggatagaagg aataaatgta
     2641 taaatgggtt gattgttttt tataaataga aatttcggtt ctttttcact gatttcttga
     2701 taatattctg ttatttgcgc caggtcattt tttaaatctg tatgtctata tcgttcaggg
     2761 atcaatataa cagggtgcgt ttctttttcg gttatttgga tcaaccttcc agagacatca
     2821 atcaaaggaa atttcttaag ataattcaga tttattttta tccctctgcc aaactgttca
     2881 ttttcttcat tcaagtaatc tgatacattg actaatatac ttccgttttt attcaaagtt
     2941 tcatataatt cctgatcggc tgctgatgca aaacgattgg aataaataaa atccacgtgg
     3001 tttttcccga tggttactgg ataaaataca tgatagtcct caccagcgat ttcttttggt
     3061 gtttcatggg aaacagctaa ctgtgaagaa gaaaaaacga tagttgctaa ttcgacattg
     3121 gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa aaagaatgta ttggatatag
     3181 ttgaacgctt ctcttaacga agcgattttt tcaactaata aaagagaaat caaagagata
     3241 ccctatattc cgctgaacaa gaaaacgtac atctggaaca aataaagaaa ttgctcctta
     3301 taatacataa aaataaaacc accaatgatt cctgcaatta tgaatgtaat aatgattttc
     3361 tttttcccca ataaacacca caatacttga tgtaatgaaa agccattatt caacaataaa
     3421 ctgatttctt tattcatcaa taagaaaaag gatgataaca aaaggaataa aagaatcaat
     3481 ccaatagtta aatacactcg ataattatat acgtctgtac ttgaaataac cgaatacgaa
     3541 tagctatttt caaagtcttc aaaaatatta gtggcataat cagtgtgaaa tgcttgattg
     3601 aatcgttcca caaatagtaa aatgaaccgt tcataggctt ctttttctct cgtcatcaag
     3661 aaaaattcac cttcaaaatc ttttattttg atcgattctt caatggacgt tacgcgaaag
     3721 gaggtattag aatagggtac cttctcgtga gaagatttga catccccttg ataatacaat
     3781 gtcatttcaa gctttggctg tatcttgaag ttccaagatg tatctacttc attggattca
     3841 tttactgttt ttttcatata agaaagctca gtttctgatg agacattttc aaataccatg
     3901 attagctgct catattcttc ttgcccaata aaatgttgtg gcaatcgaat cggagtataa
     3961 gtatctgttt gatggaaaaa ttcatttgtc cgatagagta cctgtttatt tataataacc
     4021 aaggcaaaac cactcatgat accggtcact atgattgtga gtaaaactgt gaactttttt
     4081 ttgattatca ccactctccc ttacttaaat gattctttga taaaatcata ccgtttttca
     4141 tcaaaaaaac gtatctatca aatgtatagg tcatgtagaa taaatctata tactgtagaa
     4201 tcgaaaggag aagaagatga acatcggaga aaaaatacga acttaccgca gaaattgtaa
     4261 gcttacgcaa aaagaattag gcgaaaaaat cggtgtttcc gataaaacaa tctctagttg
     4321 ggaaaattct cgtacaatgc cagaccttga aatgctgtca cttttacatc agactttagg
     4381 tctgccgatt gattttcctt ctattgaatc agctaccgaa tcaatagaac caacattgcc
     4441 ggatgaacct cttgttatca agaaatctta ttcatcaaaa caacccttct cactcaaaaa
     4501 gacactatct ttctttctgc tcgtcttttg tttggtctat ctttttctta tcaattatca
     4561 atataaaaat gaaacggttg atcgattcaa tccttttttg aaaccacaaa tcggctatac
     4621 cacactccct gtaggatacg tggaaaattc catcgaacat tgggtatgta atgatatctt
     4681 tgttcatggt acatggctgt cttttcacgc tggagaggta gaaaaaggta agaatcatgc
     4741 tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc ctgctcactt gggaagccat
     4801 tccctcagat taccaagaaa tcctcatcca agactatgac gatgccttaa aaagaagccc
     4861 tcaggagcca gataggcatt aagtaggaga tgaacgtaga gatagaaaaa atcaatgcac
     4921 atgatcttat caaaaaaaaa caggggtaca aattaattga aaaagctttg agatgaaagt
     4981 tcctaaataa aaaagttcag aatgataggt aaaaaaatga ctaactattc tagtattagg
     5041 ctatttgtat tattcttctg ttatttttat atcaaaaaat ccagaaataa ctaaatatag
     5101 gtatttctgg cttatagtat aatatttacg ccacagcccc caatgattct ttttggaatc
     5161 atgatcaagg acggacatac acaaaaatat tttgcccatt cgatccgaat agattaacga
     5221 ctaatctagt aagtaatata gattggtacg tagtcaaacc agcaaatgga aaagttgtct
     5281 attccacccc aagggcttta aagacagcat cttccggcgt ttgataaaaa atcaaattaa
     5341 aggtacccat taattctggg ggtacagttc caatatcagc agcgcttatc ataggtacaa
     5401 gtactttttt cgctccggca tcttttgcta cttgtaaaca gttagctaaa ttatctacct
     5461 taatcataga accaccaatt gtaatatccc ctaaaataac taaacttggt aatacaggtt
     5521 tatttaagga aactgagcat aaagctatta tagttgataa cgataatgtt tttgtcatac
     5581 caatgccatt catatcttga acatgaacga caaaatcttt tgtcgtcaaa ctaatagact
     5641 gactaacact tccataatta gctcttaaat atcggtatgc cgaatccatc gcttctcttg
     5701 ctccagcatc tgaattgatc ccagtacgtt caaactttcc atttcccgaa gtcatttgcg
     5761 tttccaattt ataggtacct aattgcccag ttttgccata agataccgtg tacaaatgcc
     5821 ctggcttccc taacccttct ggaatcaaag ttcctccacc ttgttcagga acagaaacat
     5881 actcttctcg ctcttcttct aaatcaatgt atgaaaaatt gacatcataa aattccattc
     5941 caccaatttt ctttaattgt tcttttacac gacgacgcat ttcaagagca aatcttagta
     6001 tttcttcgac atcacttttt tcaaatctac catcaggata caacagtttc atcatcccag
     6061 atacaatccg acgaactgca atcgtatccc gctgatttaa ttgagaacca agtttgaaat
     6121 atttatcaaa actatcgcca aaactaactt ttcgaagctc acgcatgact tcagaaaaat
     6181 aatctgtaat gaagccatag tcatttgtaa aaaaatctgg tcgatattta ggaatttccc
     6241 aacctggtaa ataacaatgc atccgatcca aaaaggctga atccgtcccc ataacctccg
     6301 gaaatggctc aaataaattc gaagttttca atattacatc aacgctttga ttaatatttc
     6361 ctacaaaagc cattgatgct gaagcattct tttcttcttt gcctctagca aaagatcctg
     6421 aagccatgta atctttcatg atttgaacgc catcattatc cttaaattta atcccagcaa
     6481 cttcgtcaaa agcaacacag tcccacatac ctacaagtcc cattgtacga ctagccatat
     6541 tatagaaaag gtttgctact gttgtttgtc cacctgaaac aaggattgag tttggtgaaa
     6601 tttctttata tacatgcgat ttacctgtac cacgtggtcc tagttcacag agattaaaat
     6661 tattttcaat taatggaata agtcttgcta aatgaagcca ttttaccctt ttattaaatt
     6721 gtgtaggttc cataccaatg gaccgtagaa gtaagtcaat ccattcattt gtagtaaact
     6781 ccttgcgagc catcttgtaa gcctcaatgt ctaaaccagg catttggatt ggatcaacct
     6841 tttttgcaat aaaagggcta ttccaacgat cattctcatc ataccaatat tccatcgaaa
     6901 caatacacca gatcccccca cctaataatc gatcaaattt tttcggatac tcttcactaa
     6961 tttgaacatt ttttaatccc aggttagaga attctgcttc ataacaatct ttcttttcat
     7021 ttaaatgtac agttacttta tcaataactg tataaccacc caattcacga atttttgctt
     7081 ttactttttc tgcttcatca ggacgcacat agtttcgagc aagaatattt ttaacatttt
     7141 ccacaccttt ttcaaccaat gtttcatcca atgacgaagc atacatacca agtaaatatt
     7201 ccaatacata aacaggtaca ttcgcacctt ctttaatttt ctttgttaaa tcttttctga
     7261 caatctttcc gggaaagaat tcagataact ttaaaatttc ctgttgttcc actagaattc
     7321 ctccatgtgt tcagtcatta taatagcaat taaagtatat catacttaac cagtaggctt
     7381 gataatacaa aagaaaaagt atccacaaaa aattgtggat acctcgtttt tatatatcaa
     7441 aatcaaatga ttctgcaaaa ggattatcta taatgaattt tattcgctcg gtcaaatcat
     7501 ttttttgatt ttttatcact aaataataag tatcttcaac tgtatatcga cgatttacaa
     7561 attcaaaaac aaatttagta aaccgctgat ttgcatcttc tgtttttgaa tccgcaacat
     7621 agatttcttc atttgagata cgttcaccat attcatcttc aaaataaagc cagaaagatg
     7681 cggggtcata aacatcacta attgcatttt tttgatagaa ttctaacgtg gttgataatc
     7741 ctgtaattct gcggttgcta gtcattaatt caattgctac tggttccttt gcattgctac
     7801 ctttggctac cgtaatttct agaacaggga ctagcatttc ttgaatagat gaccctccat
     7861 gaacgtagct ttgtcccccg ccagctttct taaaaatact tgaagtaatc gggacacaaa
     7921 tgtttctagt gtcatcattt cctaaagaat ctcccaaaga gtaatttttc gtaccaatta
     7981 tttcgtattc ttcttcacta agaataaaac gacgttctac acgatctttt tcatttctag
     8041 atggattttc aatcttatct acttcttcaa cgtggcttct tttataaata aatccatgat
     8101 cagccgtaac tataaaacga tagatactgc catcattatg caagcgatta ataagtcggg
     8161 aaatttcgct tatcgctaaa tcacaggcct caaaaactcg attttcattc gagtgatcac
     8221 ctgttctatc cacttgatcg tgataaatat aaatgactga tttgccatta aatacttctc
     8281 gtacctcagc cttattcatc ttaaccacat catcataatt taaagctaca gcatttgcat
     8341 ctcgttgctg gagaatcgca tttcgattgg ataaaccatt cgtttttttc ccatcaatca
     8401 gtacttctgt tccttcattg taagttagtt gattgtttgg taataaggcg gctttaccga
     8461 attcagtaac tgaaggtaat acagaaagaa agctagccat gtttgttgaa tacttctttt
     8521 ctctaactaa cttacgttct aaattcatcc ctaactcata tcgaaaagca tctgaaataa
     8581 taactacagt tttaacagag cgtttcatta cagcttgatt ataaaatttt cgaatggatg
     8641 gacgattctt aaattcaaaa gtttgattcc aaatttgtcc tgattgatca agaaacttcg
     8701 aataatttgc ttcaactaat cgatacaatt ggccatattt ttgcttttct tctatatgat
     8761 cactattcca aataaatttc cgataatgcg tatcaaccca atacatttcc ttctcatatt
     8821 gagttagcaa attatccatg ttcatcggca agcacaatct gtttttatcc acaagataat
     8881 aagcatgttt caataattga tactcataaa tataatccga tgcgaaatgc attttttcgc
     8941 gctgctttaa actatcaaaa attgtttgtc ctgcaatttt cgttgtagtg tcagaagcct
     9001 ttaatcgttc tgcataaaac attaatattt ttgcatgaat ctcagaaaat acgtctgctt
     9061 caattagatc atcaatttct atactgtcta ataatccatc accatcaata gcctgatata
     9121 cttttttaga tagctgttca aagctttcca tgtaacgtgt atcattcata aactgatcca
     9181 tgaaagtaat aatgtttgct ttcaaatgta gtatttcaaa tcctttgaac ttcttcggca
     9241 tctcttgatc aatttgataa taaaaatagt tcaaataaag acacatcgtc agcttttcca
     9301 atgttggctg gctatccgta aagccaaatt tcatttgaat aaattcccaa aatttagctg
     9361 ttaactgata cttatcaagt tcccttaaag gattctccct actttctgta aagcttttta
     9421 gaactacacg cagtaaatca tttaatcgaa tacttttcga tttcaacaaa acagccatta
     9481 tcattaattc aatcgtttct gatttattaa ttgtcatatg cgtactaaat tcaccaaaac
     9541 ttctcatgcg atcctttgca ttaaagaaat tacgatactg tttaactgtc tgatgaaatc
     9601 taagtggaat agccaattca ttcatgataa tcgctaagcg gtctgcacta aattcctctg
     9661 aataattcaa tacactcaac aaatgattat cttcatcttt atcatcccaa cgaggaaatg
     9721 gcgcatagat taaatagtta gtttcgggat cttcatattc caataaacgc ttagttttaa
     9781 attgtgcgtc cttttctaat cgatagattt ttgcattttg aatgaccaat gagtcaattt
     9841 cttcaagaaa atcctgattt gaatcaaacc aaaaaatgat tttcctacat ccataattag
     9901 gaaaacgttg atttaatttt tcttcaattt gttgcgtatt tatatctgtc atcaatctca
     9961 cctaatcgtt ctctttctat accccttata ttgacataat ttggataatt tcgcacgcaa
    10021 tttaagaata aaaaagaaag ttgttagaaa acaactttct tagataacta tttttttggt
    10081 tctttgaccc cttttgcaaa aatttggaat ttctcgccgg ttttaggatc acgctgcaac
    10141 ttatcataat tgacaattac accatcatct aagtctaatt caattcgttt taatgctaag
    10201 tgacccaact tctcgtcata ctcacgaact tcttttaatt gcttctgaat ctttacaatt
    10261 tcttttgtca ttgcatttct ttcttttggg ttatcacttg ttgaaatttg ttgctgacgc
    10321 aattcgattc gattgtcata cgctttttgt agttcgtgta ggtattcagt acgtactttt
    10381 cctaattgat ctggtgtata gcgatgcaag tacattaacg ctttaaagcc attttgtttt
    10441 cctgagtcca attgccaata aattggtcga ttggagtatg tttttaaatg gtctttgtaa
    10501 aaatcttgta cgaagtaacg acgaataatt tctcgacttg tccctttgcc acctaaagtg
    10561 tcagcgataa actggagatt ttcttcaagg gtttctttac caaaagtaat ctcaacgaaa
    10621 tcaatgaaac gattgacaat gtcaatttgg ctatccttga aatactcatc atcggtgatg
    10681 agtaaaacat tatcagtatc aggtttaaat ttttgatagc gggaaggatc ccattcgccg
    10741 ccagcgaaga tgagaccatc ttcgtctagg gagtaacgac cgaagataca acctattgaa
    10801 taagaaataa aatccttaat ttccctcgcc ttatcaggta accttgcaga gatatattca
    10861 tcttgaacct cttttgttaa ttctttttca agagaataat gattaatgaa tatgtcgttc
    10921 agttcctctt cattcgattt tagagtatta aattgattaa ccaactgcct acttagacag
    10981 ttaaatgcct ctgagagatc ttttttaaac atataactta acaaaatgtt tcttctaaac
    11041 tcgtatgacg tttcaagagc attatagtct tttctagcta tatcgatatt ttcatccact
    11101 ttttctctta ttttttttaa ttttctctca gatgtaactt taagaaaagg tagttttccc
    11161 attggtcctt catggtaatc caaggtggga gaaataaaag ttaaaaagtg attaactaat
    11221 ttagagttta catagccaaa tatttcttca aagtcagtta tatcctttga gaacagaaca
    11281 gatcctttag tttcaaaaat tgcctgattc tgcaatctaa aagatacttg tccagatgaa
    11341 agtgtcgacc aagtaattcc ctttttaaaa tagtaattaa gattttgtgg tctagatttt
    11401 aacttcccat tttcattaac ataatttttt atttcatctc catcattctc ccagttaacc
    11461 acgtattcat tgtttccata ccattttcta aaacgaccac ctttagttgt aggaaaccat
    11521 ttttttctag actttttagc gtcctctaat gttttagcat caaactttat tttgttaaat
    11581 tctacttcaa accaataccg caaaaattta ttgttatttc cggttgccaa cccttgtttt
    11641 gcatctgcaa catcgttagt taattttcct tgagcaaaat cagtgattac gttatcgctt
    11701 gcccagtaag caatcggcat tcctgggatt tttttgaagt tgtcttgatt agtactataa
    11761 aaataatcgc attcaccatg ctgatgaatt gcttctaacg ttttaatttt ttgaacttcc
    11821 attccacctt taaaatctga taattttata tagcttccat cttgttcatt tgaatttttt
    11881 atcgtaaacg tacaaattgg aacggttgct tcttcaaaag ctgaatactc catttgaatc
    11941 aaactagaaa tgtttctttc attaattaaa aatgagcgta acttttcata gctagaaatg
    12001 aacatccaaa caaatggcgt cataaaggaa gcatatccat tatcaatcag catttggcta
    12061 ttcaatttaa taaacacaga aaatagatct tttttgacat cagaatagtt atcatttaca
    12121 tattttttta atttcttatc cattttatta agataaggcg gattagtgac taccgctaca
    12181 tatttatcaa ctagcatttc agccacgtca agaatgtgca agtatttttc ttgtatttcc
    12241 ttcatcccat aaagaatatc catattcgta aattcgttga attttgatat atactctctt
    12301 acttttccta agtattcttt agttattgcc tgattatttt tatcgactaa cttaatgatt
    12361 gatccaagtt ctttcccatt ttcaaattga tcaaaaatta actctttgta ttcattccag
    12421 cgttcactat acttatcatt gttcaattca ctaatacgct cttgatactc gtctgtcact
    12481 ttaggagaat caataaactc tcttaaattc ggcgatagac tttttttcaa aaaaagacga
    12541 tcgtattgac gccctttcat cataattgca aaataagaca attgatacgc tcttttatcg
    12601 atttcaagac catataaatt gtattcaacg attgcacgtg cagcatcacg ctcagaatat
    12661 cctaacgtaa gataaatttc catcaataca tcgaaggcgt aaactaaaat atgcccagac
    12721 cccattgcgt tatcgataat ttttagttct tctggtttta aattacttac agatgttgct
    12781 tgttcaaaag catatttatc tggaattaag tatttcaact tatttcttaa ttcactattg
    12841 ggataatgat ctaaccaata ctttcctaaa ctattgtcaa ccatataacg aacgacccaa
    12901 tctgtggtaa atagttgagt tgctgccgga atatcttgtt ttttgacaat ccctttataa
    12961 atattcacca cttgattatg tggctcacta ttatagtact gatacaacca cccaataatc
    13021 tctacctgtc caccagcttg aacatagaaa tcatcctctg gaatacttaa aagatcagcg
    13081 attacaccat cttttttaat ataagaagct gtaaacaaaa gttctgtata atcttctact
    13141 tcttcaaata gctcaggtaa gtaatcattc aattgattac agatttttat aaaaatcaat
    13201 tgatacagct tgtccattgc ttctgctgaa ttatctgttt tccaagttct gacttgttgc
    13261 tgttcattac tagataattc ttcaaagata tcggcatcat aaatatctgt aataatatct
    13321 ggctcaattt tcccacttat ttcactagat aatacacgat acgtgtcagg taaataatca
    13381 tttacctcca taaaccgaat ggcaatcaat cggttaaacc acgtataagc aacctcttcg
    13441 attaactctt gaaaagcttc tgaataagaa gtgacagaag cacgttgttt tagttctgca
    13501 atcaatttgt ttctttgcgc tatttctttt ccttgaattc ttattcctga tttttcgatt
    13561 tcttgtaaat tgctatcaat tttcttcaca tcttcaattt ttgattcatc aattcctaaa
    13621 cgttttgctt tagtcattac ttcatcaatt aattttctac gggcattgac cgcgaaattc
    13681 tttatcgctt tcttatccat ttttctttcc cttctatatg taacctttga aaagacaggc
    13741 acctttttat gcctgtcttt tcactagaat tgaatcgtga tttctgtatt ttcttgaagt
    13801 tgttccttca gtttttgatt aatttcagct gttaactcat caatatctgc tgctgactta
    13861 atagttttaa catgaacgtt aagtgttctc attggtactg ttttctgttg aatagtaggt
    13921 tttactatcg ctatttcttc agcttttgct gcagagtcta tttcatcttt ttcagtttca
    13981 cgaatcaagt ttgctgcttc aatttgttta cgtgcttgtt cttcttgacg tctgcggttt
    14041 tcttcattat cgaaacgttg attaaagatc aaatactgtt ctgctacgtt taatgataag
    14101 aaaacaagat catctatagt attcgcttgt tcaatttttg ctttcagttc ttgaaattct
    14161 tggtcaacta ttggtacaaa ttgatcctta aaatcttttc cttctaataa tttaaatgtt
    14221 gcttgttttt cttccgttaa tttatttaat atttcctgac tttccaactc taacagttga
    14281 ttatattgtt caataaattc ttgaatcaac tcatttagtt tcatcatatc tgaataagga
    14341 tcagcctttt tcaaaatggt tttcatctca tgaatcaatt gatcaatttc ttggctagaa
    14401 gtttgacgcc gacttttttc gattaatcta attttatctt gtgcattctc ccagattttt
    14461 ttctgagtac tattttgctt gtggaaattt ttaattttat catatgtttc agcccagtcg
    14521 aataattcat cgcgatattt tttgattgta taaaaaaagt cttcaacatc tgacgtttga
    14581 acaattactt caagatattt ttttatttgc tcatattcat aagcactagg gtaggtataa
    14641 gtgttttgta aatcaaaata agagcgtaaa ttgtctaatt gaccttttaa atatttcttg
    14701 aaattttcca ttgtatgttc attgttattt tcatcaacaa tattacgact ttgaaacagt
    14761 tctctaccga tttcgtcaac caattttttt gcttttggcg aaatcaccgc gcttggttca
    14821 atttttatct tttcactttc tcgtcgtttt tgaataatca ttacaatact atttggatca
    14881 tccatccgtg aaatttgtac attgttataa agtaaatcaa ttttaccaga catgaaaagt
    14941 ttagcgatgc accattccac atcaagttct gtgaatccat acggttgttt tgtttcaaaa
    15001 cgatcacgaa ttgttttcat cgtaatcgtt aggtgatttc tcttttgatc attgataaaa
    15061 ttgaaaattt cctgaactgc ttgctcattt gatggatctt caataatttc atttttgctt
    15121 tctttcacta aatataagat atcttctttt tctttaggcg catcaatttg gtttaattta
    15181 tggtatactt cttcagccat tttctgaagt gctgattgga attttgcttt aaagtcggag
    15241 cctgtcagtt gaatttctgc accgttatag aaaaattttg cagatttcaa cagttcattt
    15301 aattgatcgt tgatacttgc acttagttcc tgtaattcac cagatttttc cgaacgaatc
    15361 gcgtcaaagt tctttactac actcgaataa gtcgttgtta aataaagatt cattctagca
    15421 tacattgcta tatcatcttt aaattcttta tctgatggca attctacgaa taataagtta
    15481 cgggaactct taagcgcaag ttctgcctct tgacctgcta aatcagagta tggtgaaata
    15541 aaattaactg tcatttcagc atttccagct tgcttgtatg gacgttgatc taacacttgt
    15601 gcataagtaa acgtataccg gtttttaaat ttatttccac ctgacatgcc taatttttca
    15661 taggcaaaac tatttcggtc tagaacgccg tcaaaaattc gtttagatag ttctacgatc
    15721 aaatcactat ctgtaatatt ttgattttta atctcacgag taatttcctg ttcttcatca
    15781 gtttggaaaa tataactatc taagttcttc cgcacaaagt tttccttaat taaaaggtcc
    15841 aatgcttctt ctactcgttt tttagtttcc aggcggtctt cgttgatatt tcttatcatc
    15901 aagctcgtaa tattttcaac atttggtttg atggcttgat caacatattt aatcatgaat
    15961 aaaattcgta agacatttac gttaaagtta tctatatcag ttggtctttg aatatgagaa
    16021 ttatttgtag cttgtgtaat cactcggcta attgctgtat caatccaacg ttctaacgag
    16081 taataaaact gatctaaaga aaccaaactg gttgtatcat tttccttatt tcgttcagcg
    16141 gcttctttaa acattgctag taatgaacgt tcaccagacg ataagtttga tccttgatag
    16201 ctgtgattac gaatctcggt tagaatttgc cccaataaat tgaattgata gggaataaat
    16261 ggataaactt ctgcgtaatt ttcagcatct tggaagagct ttaattcagg actatcaaca
    16321 aagagtaatt tatttttcaa ttctgtttga acagtttcaa attgtaaggc taaagaatca
    16381 ttggcaaact cttttttctt caaaatacgt tctttaatta cttcatcggc atttgctgaa
    16441 gataaactca cagttgtggc aaaacgatcc ttgatcttag aaaaatcatt atcagaattg
    16501 ttatcaaagt ttgttacgtg atcaatcgct tcctgacttg tcactaatac ccaagcctta
    16561 ccttcacaaa ttcgacccaa atcttccaca acggtttgta actgcaaagt caactcacga
    16621 ttaccgccga tatattgtcc gacctcatcg actagaaagg caatatgatg gtctgcttct
    16681 ttggaatcaa gatagctttt aactagattt gcaaagtcat caatgcttat taaataattt
    16741 cctgatgctt tttctaaaaa attatcaata gcggcttcac tcataaaatc gattttaagt
    16801 gccgcttctt tgatatcatc ttggatccaa tcaaactcat ggcgctcttc ttgccatttt
    16861 gccccattaa tctcttcaaa agcagctttg aatgcatcaa acttttcttc tttaattaac
    16921 cgacgttcaa aatctgctag atgtggaatc gtgcttaatc cttgcatttc gttaaatacc
    16981 tttaaaaaaa cattcacgat tccgtttttg tcttttttac cacctgcatc actttttgaa
    17041 tcaatattaa acagaataac atctgtattt atttgtgagc accgttccat cgttgccaaa
    17101 gtcagtggat ctttgatttt cccatcttct ttgaagtaat caatggcttt cttgtcattt
    17161 atttctcgat ttgcgagcaa gtaagaaata atttttaaga aatgagattt acctgaacca
    17221 aagaacccct gaatccaaac acccatttta tctgttggtg tattgatact ccgttggtag
    17281 gcatcaaaaa ttgttttaaa gtgtttttgt agttctttcg taaccacgta ttcttctaat
    17341 tcttgttgaa cattttcatc ctttgcttga ccgaccttaa taactccttg aatatttcgg
    17401 tcaattggtt ttgcaaaaat atctttaatt agcatttaat ctcctacctt ccatcccaac
    17461 taaattctaa atgctcgata ataattatca tctttattgg tattaagaat tgtcaaactt
    17521 aattcatcat attcccctgg atacatcatc acaactggta cgcgatcaaa tacctgatgc
    17581 attttattta aaatagagtg cgctctaatt aatggatata attcgccaat tcctgtcagg
    17641 aacacaactg atttttctgg tgtatgggca tccacatatt caaaaaatac attttcttcc
    17701 tcaggtaaat ccattttcaa taaacgacca atgctttcca ctaatttttg taaaccacgt
    17761 cgttcttcca tcttaatcgc tttttcagtg aaaccgcgtt tttctagata ctctaacata
    17821 atcacataga gattataaac tttcacttca taattatcac caacagatgt tccagatata
    17881 tcattaatat aatctcttac aattaactcg tctttcggat cgtagtcaaa gatataatag
    17941 cctacttcat tacctaaacc acgattcaat aaaaaatctt ctttttgaat tacttcattc
    18001 aacttttcca aatgctcttg aatcgataat gacattataa aattcctccc agtgctagta
    18061 caatatcttt acgattcttg tcaactaacc attgaatcaa tcgtttatct aataatatcg
    18121 gagtcattcg tccatattct ttttcccctc tgactaatcc tgtttcaatt agattcgttt
    18181 gataagcggc aataagccgt ttaatcgtat aatctttcca ttttgaaata tcttctgaac
    18241 ttctccgctt ctctgtaaaa aaagaacggt aatcaactgg tgttaattgc aaatttcctt
    18301 tacgtagatt ttcagcatat acctcagtca taaattcatt aaaaagatca tcaatcataa
    18361 ggatggaaat caaattcact aacttttgat tatctatatt taactgattg aaaagttgca
    18421 atccagcttc atccagttga ctaactctcc ttttcacgtt ctgatagatt tctttttgac
    18481 ggttactgct cactgcaaaa aaaatatttt catgttcatt tttctcttta atttctatat
    18541 ctgtatatcc ttctagtcgt aattgaatat ataacttgaa ctctgaaaac caaaagcgtt
    18601 tagaaactag accagcagaa tattttttat tcaatagatt gcctcctaat catctataat
    18661 tcaataagta aacaagtgtt ttttgctcat cgttagcatt cattttttag tatctactat
    18721 acaatacata tttattata
//
LOCUS       DADQRX010000055        18228 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.056, whole genome shotgun sequence.
ACCESSION   DADQRX010000055 DADQRX010000000
VERSION     DADQRX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.056"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            442..999
                     /locus_tag="J8V14_001774"
                     /pseudo
     CDS             442..999
                     /locus_tag="J8V14_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1057..3099
                     /locus_tag="J8V14_001775"
     CDS             1057..3099
                     /locus_tag="J8V14_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBA0921878.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3208..4359)
                     /locus_tag="J8V14_001776"
     CDS             complement(3208..4359)
                     /locus_tag="J8V14_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBA0921879.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4513..5043)
                     /locus_tag="J8V14_001777"
     CDS             complement(4513..5043)
                     /locus_tag="J8V14_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921880.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5142..5492)
                     /locus_tag="J8V14_001778"
     CDS             complement(5142..5492)
                     /locus_tag="J8V14_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBA0921881.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5649..7178)
                     /gene="ahpF"
                     /locus_tag="J8V14_001779"
     CDS             complement(5649..7178)
                     /gene="ahpF"
                     /locus_tag="J8V14_001779"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBA0921882.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7188..7751)
                     /gene="ahpC"
                     /locus_tag="J8V14_001780"
     CDS             complement(7188..7751)
                     /gene="ahpC"
                     /locus_tag="J8V14_001780"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBA0921883.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7895..8557)
                     /locus_tag="J8V14_001781"
     CDS             complement(7895..8557)
                     /locus_tag="J8V14_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0921884.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8741..10387
                     /locus_tag="J8V14_001782"
     CDS             8741..10387
                     /locus_tag="J8V14_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA0921885.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10378..11460
                     /gene="uxuA"
                     /locus_tag="J8V14_001783"
     CDS             10378..11460
                     /gene="uxuA"
                     /locus_tag="J8V14_001783"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBA0921886.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11568..13121)
                     /locus_tag="J8V14_001784"
                     /pseudo
     CDS             complement(11568..13121)
                     /locus_tag="J8V14_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            13269..14410
                     /locus_tag="J8V14_001785"
                     /pseudo
     CDS             13269..14410
                     /locus_tag="J8V14_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14536..15615)
                     /locus_tag="J8V14_001786"
     CDS             complement(14536..15615)
                     /locus_tag="J8V14_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA0921887.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15724..16164)
                     /locus_tag="J8V14_001787"
     CDS             complement(15724..16164)
                     /locus_tag="J8V14_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0921888.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16221..16631)
                     /locus_tag="J8V14_001788"
     CDS             complement(16221..16631)
                     /locus_tag="J8V14_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBA0921889.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16812..17246)
                     /locus_tag="J8V14_001789"
     CDS             complement(16812..17246)
                     /locus_tag="J8V14_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA0921890.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17346..18194)
                     /locus_tag="J8V14_001790"
     CDS             complement(17346..18194)
                     /locus_tag="J8V14_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBA0921891.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6252 a         3375 c         3286 g         5315 t
ORIGIN      
        1 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
       61 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      121 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      181 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      241 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      301 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      361 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      421 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      481 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      541 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      601 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      661 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      721 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      781 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      841 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      901 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
      961 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1021 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1081 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1141 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1201 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1261 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1321 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1381 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1441 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1501 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1561 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1621 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1681 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1741 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1801 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1861 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1921 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     1981 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2041 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2101 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2161 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2221 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2281 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2341 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2401 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2461 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2521 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2581 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2641 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2701 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2761 ttgattgcag atgataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta
     2821 caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct
     2881 ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa
     2941 caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt
     3001 tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca
     3061 tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag
     3121 taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt
     3181 ctctttcaca acttcttttt gatttactca cacttcataa actttttttg taaaatattt
     3241 gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta
     3301 ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc
     3361 acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg
     3421 agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat
     3481 aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa
     3541 tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac
     3601 ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac
     3661 aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga
     3721 atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa
     3781 gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct
     3841 agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc
     3901 atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc
     3961 ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat
     4021 cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa
     4081 gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa
     4141 atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg
     4201 gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag
     4261 taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt
     4321 tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa
     4381 aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa
     4441 aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat
     4501 aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg
     4561 gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa
     4621 tggattgaac aataagacga atccgccaat caacaacaga atactactat aaacactcca
     4681 acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc
     4741 taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag
     4801 agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct
     4861 tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat
     4921 cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat
     4981 aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa
     5041 catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa
     5101 tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt
     5161 tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc
     5221 atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa
     5281 cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt
     5341 agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact
     5401 tcctctttta tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca
     5461 aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt
     5521 atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa
     5581 aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc
     5641 ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa
     5701 cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg
     5761 ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg
     5821 atcgttctat ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa
     5881 acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga
     5941 ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag
     6001 actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat
     6061 acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg
     6121 ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt
     6181 cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg cgcacctgtc
     6241 gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat attaataaaa
     6301 tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat agggtaacta
     6361 cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc
     6421 atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc gacaagtcct
     6481 gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg tccaccgcca
     6541 atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa agggccagtg
     6601 attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc tccatttaaa
     6661 aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa catcccacct
     6721 tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa tgcctgtaca
     6781 acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa tgtttgtgtt
     6841 atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc actaatttgt
     6901 aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc aaaaacgatt
     6961 ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga atctcttttt
     7021 tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc tagtttttga
     7081 ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat cagtgctaaa
     7141 tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta aatctttcca
     7201 acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc cggacaaact
     7261 tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag tgcactagcg
     7321 tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc tggatcaata
     7381 ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa aatacgagaa
     7441 atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc atctgatgta
     7501 tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac ttcaacacct
     7561 aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg acaaacaaaa
     7621 gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc tttttcactg
     7681 acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat ttttttccca
     7741 atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt gtaatttagt
     7801 tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt
     7861 taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac
     7921 gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc
     7981 gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga
     8041 tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg
     8101 tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt
     8161 catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt
     8221 cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac
     8281 attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa
     8341 caagattaat tttggaaaca tacgtaccac tttgaggtat cgtatacacg agttcttgct
     8401 gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca
     8461 gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa
     8521 tcgcttgata agcttgtgat tgcaagctgc ttttcatctt tattcctcca aaatcatttc
     8581 tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt
     8641 aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac
     8701 ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg
     8761 gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc
     8821 atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct
     8881 ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac
     8941 tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt
     9001 tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt
     9061 agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat
     9121 gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg
     9181 ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa
     9241 tggtccaaag aagccatcac atacaatgag tattcttgct gtgctacttt ataaacgttt
     9301 tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag
     9361 tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca
     9421 aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat
     9481 tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt
     9541 ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac
     9601 agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga
     9661 aaaagctggg gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt
     9721 gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta
     9781 taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg
     9841 gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct
     9901 ggaagaagta attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat
     9961 cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat
    10021 ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg
    10081 gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc
    10141 actattgaca gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc
    10201 acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct
    10261 ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc
    10321 aaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg
    10381 gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga
    10441 cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta
    10501 tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta
    10561 aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag
    10621 cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc
    10681 atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta
    10741 gaagacggct catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa
    10801 atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag
    10861 cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt
    10921 cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc
    10981 aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta
    11041 aagaatcgag aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc
    11101 actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca
    11161 gaattttgcc aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct
    11221 aatcgagatt tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc
    11281 atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga
    11341 atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct
    11401 gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag
    11461 tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa
    11521 atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact
    11581 tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct
    11641 gagatcctca taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag
    11701 atttttttga tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag
    11761 ttctccataa aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta
    11821 acgatcagat ctttactgat ataaaaatca cgcgtatagt tcacaccaat cacaattcgc
    11881 ggctgttttt tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt
    11941 tccttttcta aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat
    12001 tgttcaatgg cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta
    12061 ggaaggggcg tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg
    12121 aatgccaatg agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa
    12181 agggactcca ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt
    12241 ttgtattgct ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa
    12301 atattcacta agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca
    12361 gagagatcaa taaaaaagtc aatatattca tactctgtga attctttttg tacgtctttc
    12421 acagaagttg caggaaaatt ctctttacat atttttaata gaaaatataa tttatctatc
    12481 ataccgctat ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc
    12541 actacttgcg gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa
    12601 atatctaatg ctgcatattg aatttgtacc tcttttccag aaaaagactt tctgccatta
    12661 ttagaaaagg agatagacaa accgtactat gctaaaaatg cattaatctt tttcattttc
    12721 gtatacaaat aagaggcact atagtttgtt tgctgactga aactgataat cgaatttgtc
    12781 tgacagctga acaaagaagt caagatctga taactgactg attcgttgca gtatcttcca
    12841 aatagacgat aatagcagta agccggatca attccttcaa aattcaactt taaaaaaggt
    12901 ttttgcttat caatatgcag ttctgttaga ttgagacgct caaagtcttg ttctaattct
    12961 ttgatgtata gtaaaaaatt tgattcagac atatggaaat gatctgctaa atattgtgta
    13021 gtaataaaga aacgatgttc agataggaaa agaaaaatat ctaattttgt attgcttttt
    13081 ttcttcaaga taaacgtttt aatcgcttct tgagtttgca tgaaagaggc tcctttttac
    13141 ttaaattgtt aaaacttgaa aatattactg tttttttcat aaatttagac aaaaaaacag
    13201 taaacatata ggtagaatct ctactatctt atttcttgca tacaaaaaaa tcaaggagtg
    13261 agagagttat gagatattca accatcttaa tatcaacatt tgcagcctta agcttatcgc
    13321 ttactataaa tcagacagtc cacacagagg agacagaaac agccaaatgg acagaaacca
    13381 agtgggccaa cgtcaatcga attacctttc aggatgaatt ttcatccttc aattcaggag
    13441 tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc aaaaattgac tattcgggag
    13501 acttagcacc tatactacaa tcagctgtta catttgagaa tgttgatccc actttgtcga
    13561 ttggctatga cgagaacact ttgactatcc aacacaagca agcgatattt accttcaccg
    13621 ttacattaaa tcaggacttg ttaaaaccag cgctttttac tgtcaaggta tcagatggac
    13681 taccgaacga tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag
    13741 ccatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata
    13801 agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg
    13861 gagaagaacc cgatacagaa actcaaccag agccagacaa taaatcaaaa cctgaaataa
    13921 cccctggaga aaaaccagat gtaactccag aaccagatac ggattctgga aaccaaacag
    13981 taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa ccagaggtag
    14041 atccagaaga aaaaccagat gtgactccag aaccagatac ggacgcaaga gaccaaggaa
    14101 taccagaaaa aataaacaaa aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat
    14161 caaatctagc aattctaaaa ataaatgaag agcagctgaa taaaaaatca cgaatatttg
    14221 atagcgccca atcagctgaa accttaaaaa gtagcaagga tacaaccttt gctgtcctga
    14281 aacgaaaaat aaacagctac caaaatccgg agagtcacaa aacaaagtta tactgtggag
    14341 cggaatcatt ctattaagta tagcaacaat gctttctgca aaaagattta aacaaaacag
    14401 atctctctaa catttgaata atgtctgtga acgcattttt caaataaaaa gaaatatcca
    14461 gaaaatgacc ccaaaatacc agattgaaat ctgatatttg gggtcgttca tactttatct
    14521 ttcttcatat ttattttaag ggatatctgt ttcttttgga agatggttcg ggatcttttt
    14581 cactttcaat acatctaaaa agaaggtaaa gatcggaatg ccgacaatca agccccaaac
    14641 gccaaagaac cgttcgctaa caagtaagac cacaaatgta taaaaaatcg gcagttctgt
    14701 tttgctggac atcaacttcg gattcagcac ataagactca attagatgga caatcaaaat
    14761 cgtaatcagg atataaacaa catctcggat gcctccttgg gaataagcta tgaaagatag
    14821 aggaatacaa gaaatgataa cacctgctac tgggatcaga cttaaaatga aaatcatgat
    14881 cgctaaactc agcagctgat gaaagccaaa tacagccaag gcgattgtag tgagcaacgt
    14941 atttaaaaga gcgacaacaa attgagcttc taataccaaa ccaaatgtat tgacaaactt
    15001 atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat tcacttttta aaaacaatct
    15061 tgagaataca attgtttttt tcttttcaat cataaaaaag aaacttaaga tgaatgacat
    15121 agcaaacgcc actgccattt ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc
    15181 attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa gtatggatat attgtaaaac
    15241 aggatctgca tcttttggct gatgctcgta aaaatcaata acggactgga tcatatcaaa
    15301 tgtttgatga ataagcatcg ggatatactt tgtgaatgcc agatagataa ggtaaacgat
    15361 cagtgtataa atcatgatag ctaaaacacc tgtagggatt cggacaaatc gctgaatgat
    15421 ttgaacaaga tgaatcatca aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat
    15481 acttctaact aagaaaagaa caaaaacgac aaataataaa acagtgagac ggcgtatttt
    15541 ttcattcgcc aaaaaacgct gataaacggt caaatcgaca acctccaaat aacaaaaaat
    15601 aaacacgtta ttcattatat gatggaatat gaaaataaga aagagaaaga gcaaaaagaa
    15661 aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca
    15721 gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg
    15781 cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc
    15841 ttctgcttgt ttttgcaacg cagcaccttg aggagtcagc gtaacattta cgattctttc
    15901 atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg
    15961 agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt
    16021 cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag
    16081 gagggaagtg tagtgtctaa tgatttcctt agaggcggca tataaaggaa aacatacttg
    16141 attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata
    16201 gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca
    16261 gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct
    16321 tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca
    16381 ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc
    16441 tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt
    16501 tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta
    16561 cgatcgggat tatgaacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac
    16621 atttttttca taaaattacc acctttaatt atattttata caattgaatt ttacacaata
    16681 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
    16741 agacgcgctt tctcgaatag caaacatata caaaaaaata tcagagacaa aaactaatct
    16801 catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa acttacttca
    16861 cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc
    16921 acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct
    16981 ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt
    17041 tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgtg
    17101 ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt
    17161 tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca
    17221 aaatctattt ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac
    17281 tagattcttt tattatacca gttaaagaaa acgcattcaa aaaaatagcg gatttactta
    17341 ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg
    17401 acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca
    17461 tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc
    17521 tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa
    17581 taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc
    17641 atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa
    17701 acaaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc
    17761 aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg
    17821 tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac
    17881 aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaacaag
    17941 cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac
    18001 ataacgatat tcaacactaa aataactata gctcggtcaa atcctaaaac agcttccagt
    18061 aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg
    18121 atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt
    18181 ctcgtttctt tcatttatcc acttccttaa ttcattaata atactctt
//
LOCUS       DADQRX010000056        17385 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.057, whole genome shotgun sequence.
ACCESSION   DADQRX010000056 DADQRX010000000
VERSION     DADQRX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.057"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            275..2041
                     /locus_tag="J8V14_001791"
     CDS             275..2041
                     /locus_tag="J8V14_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBA0921892.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2143..3402
                     /locus_tag="J8V14_001792"
     CDS             2143..3402
                     /locus_tag="J8V14_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBA0921893.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3549..4850
                     /locus_tag="J8V14_001793"
     CDS             3549..4850
                     /locus_tag="J8V14_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921894.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4853..5704
                     /locus_tag="J8V14_001794"
     CDS             4853..5704
                     /locus_tag="J8V14_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0921895.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5717..6532
                     /locus_tag="J8V14_001795"
     CDS             5717..6532
                     /locus_tag="J8V14_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBA0921896.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6683..7849
                     /locus_tag="J8V14_001796"
     CDS             6683..7849
                     /locus_tag="J8V14_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0921897.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8079..8726
                     /locus_tag="J8V14_001797"
     CDS             8079..8726
                     /locus_tag="J8V14_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBA0921898.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8810..9754
                     /locus_tag="J8V14_001798"
     CDS             8810..9754
                     /locus_tag="J8V14_001798"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBA0921899.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9889..11340
                     /locus_tag="J8V14_001799"
     CDS             9889..11340
                     /locus_tag="J8V14_001799"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBA0921900.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11361..12197
                     /locus_tag="J8V14_001800"
     CDS             11361..12197
                     /locus_tag="J8V14_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBA0921901.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12197..12856
                     /locus_tag="J8V14_001801"
     CDS             12197..12856
                     /locus_tag="J8V14_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA0921902.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12982..13671
                     /locus_tag="J8V14_001802"
     CDS             12982..13671
                     /locus_tag="J8V14_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921903.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13677..14633
                     /locus_tag="J8V14_001803"
     CDS             13677..14633
                     /locus_tag="J8V14_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBA0921904.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14677..15189
                     /locus_tag="J8V14_001804"
     CDS             14677..15189
                     /locus_tag="J8V14_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0921905.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15445..15993)
                     /locus_tag="J8V14_001805"
     CDS             complement(15445..15993)
                     /locus_tag="J8V14_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0921906.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16082..17263)
                     /locus_tag="J8V14_001806"
     CDS             complement(16082..17263)
                     /locus_tag="J8V14_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBA0921907.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5716 a         3092 c         3476 g         5101 t
ORIGIN      
        1 ttcttctaga atatacttct acgttaaaaa ctgttaaaaa acttttactt tagaaaattg
       61 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagattaaaa
      121 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
      181 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gtttctctat cacttatagt
      241 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
      301 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
      361 taggaaaaat gatatcaaaa aagtatatgt gctttgtgga gatccatata ccataacaac
      421 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
      481 ttattgggaa attgaagtga cgagcgaaac aaggcgctta cagtatgcgt tccatgttgt
      541 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
      601 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
      661 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
      721 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
      781 ccgagaagac ttcttcgggg gagatttgca aggtgtgagg gatcatttgg attacttgac
      841 ggaccttggc gtcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
      901 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
      961 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     1021 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     1081 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     1141 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     1201 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     1261 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     1321 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     1381 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     1441 tgcattcaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     1501 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     1561 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     1621 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     1681 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     1741 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     1801 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag tatatgatcg
     1861 tgaagataag ctctatttca cacgggaact agagggacag atcatctatg tttactttaa
     1921 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     1981 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     2041 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     2101 taagggtgta acagcacgtt gaatacacag gaggaaatga agatgaaaaa aagaagtgtg
     2161 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     2221 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     2281 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     2341 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     2401 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     2461 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     2521 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     2581 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     2641 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     2701 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     2761 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     2821 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     2881 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     2941 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     3001 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     3061 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     3121 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     3181 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     3241 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     3301 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     3361 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     3421 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     3481 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     3541 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     3601 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     3661 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     3721 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     3781 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     3841 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     3901 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     3961 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     4021 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     4081 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     4141 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     4201 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     4261 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     4321 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     4381 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     4441 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     4501 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     4561 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     4621 atttcaataa tttctccatg atctatctgt ttaacaatgg tggtcccgga agcgtaggcg
     4681 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     4741 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     4801 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     4861 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     4921 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     4981 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     5041 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     5101 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     5161 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     5221 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     5281 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     5341 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     5401 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     5461 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     5521 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     5581 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     5641 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     5701 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
     5761 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
     5821 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
     5881 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
     5941 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
     6001 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
     6061 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
     6121 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
     6181 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
     6241 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
     6301 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
     6361 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
     6421 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
     6481 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
     6541 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
     6601 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
     6661 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
     6721 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
     6781 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
     6841 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
     6901 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
     6961 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
     7021 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
     7081 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
     7141 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
     7201 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
     7261 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
     7321 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
     7381 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
     7441 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
     7501 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
     7561 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
     7621 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
     7681 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
     7741 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
     7801 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
     7861 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
     7921 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
     7981 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
     8041 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
     8101 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
     8161 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
     8221 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag
     8281 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
     8341 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
     8401 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
     8461 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
     8521 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
     8581 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
     8641 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
     8701 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
     8761 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
     8821 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
     8881 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
     8941 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
     9001 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
     9061 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
     9121 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
     9181 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
     9241 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
     9301 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
     9361 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
     9421 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga
     9481 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
     9541 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
     9601 atgtggcgca agcatgttac agattggtac agagcttcat aaagaagggc cggaaatttt
     9661 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
     9721 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
     9781 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
     9841 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
     9901 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
     9961 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
    10021 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
    10081 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    10141 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    10201 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    10261 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    10321 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    10381 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    10441 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    10501 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    10561 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    10621 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    10681 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    10741 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    10801 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    10861 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    10921 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    10981 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    11041 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    11101 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    11161 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    11221 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    11281 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    11341 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    11401 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    11461 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    11521 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    11581 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    11641 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    11701 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    11761 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    11821 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    11881 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    11941 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    12001 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    12061 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    12121 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    12181 agtcggtgag tgagtgatgt actttcccct aattgatttt tccctcatgt ggacttcact
    12241 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    12301 aggaaatttg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    12361 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    12421 tctgttatat ttcgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    12481 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    12541 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    12601 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    12661 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    12721 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    12781 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    12841 ccatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    12901 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    12961 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    13021 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    13081 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    13141 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    13201 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    13261 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    13321 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    13381 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    13441 gagcgaggcg taaaatattt tccaaaagat caattgtaca aagaattgaa aagacgttca
    13501 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    13561 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    13621 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    13681 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    13741 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    13801 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc
    13861 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    13921 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    13981 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    14041 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    14101 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    14161 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    14221 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    14281 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    14341 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    14401 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    14461 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    14521 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    14581 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    14641 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    14701 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    14761 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    14821 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    14881 gtctcaggcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    14941 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    15001 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    15061 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    15121 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    15181 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    15241 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    15301 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    15361 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgatc tctcggctta
    15421 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    15481 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    15541 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    15601 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    15661 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    15721 acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg
    15781 agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc
    15841 agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta
    15901 agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc
    15961 agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca
    16021 agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat
    16081 tttataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca
    16141 aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg
    16201 caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa
    16261 ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa
    16321 attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg
    16381 ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga
    16441 tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa
    16501 gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt
    16561 ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata
    16621 catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag
    16681 tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg
    16741 tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct
    16801 tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag
    16861 attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata
    16921 atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca
    16981 tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt
    17041 caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa
    17101 ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca
    17161 tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc
    17221 ctaatgtgac aaataaacca ataataccta ataaataact caaataatca ctcctattcc
    17281 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
    17341 ttactttttt agagtgaaac gcattcattt tccattagaa aataa
//
LOCUS       DADQRX010000057        17318 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.058, whole genome shotgun sequence.
ACCESSION   DADQRX010000057 DADQRX010000000
VERSION     DADQRX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.058"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..530)
                     /locus_tag="J8V14_001807"
     CDS             complement(9..530)
                     /locus_tag="J8V14_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBA0921908.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="J8V14_001808"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="J8V14_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBA0921909.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="J8V14_001809"
     CDS             4610..5497
                     /locus_tag="J8V14_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBA0921910.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="J8V14_001810"
     CDS             5494..6663
                     /locus_tag="J8V14_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921911.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="J8V14_001811"
     CDS             6687..8042
                     /locus_tag="J8V14_001811"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBA0921912.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="J8V14_001812"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="J8V14_001812"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBA0921913.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="J8V14_001813"
     CDS             10328..10618
                     /locus_tag="J8V14_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921914.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="J8V14_001814"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="J8V14_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBA0921915.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="J8V14_001815"
     CDS             11659..12318
                     /locus_tag="J8V14_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBA0921916.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="J8V14_001816"
     CDS             12475..13407
                     /locus_tag="J8V14_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921917.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /locus_tag="J8V14_001817"
     CDS             13561..14850
                     /locus_tag="J8V14_001817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBA0921918.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="J8V14_001818"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="J8V14_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBA0921919.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /locus_tag="J8V14_001819"
     CDS             16430..17017
                     /locus_tag="J8V14_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBA0921920.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5644 a         3102 c         3774 g         4798 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgattaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcaagt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agtctttttt tgagcttaac ttacttttgt
    17101 ttcttgtctt ttttcttttc attttgtggc tgtggtttta gaaatcgatc gatctcttct
    17161 tttggtgcaa ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt atctaaatta
    17221 tctgtacgtt caaatttcaa ccccattgta aacacctctt tgtttctttt tacttagtct
    17281 atcatgtaat cgtccaaatt ggtttctaag acataaag
//
LOCUS       DADQRX010000058        16271 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.059, whole genome shotgun sequence.
ACCESSION   DADQRX010000058 DADQRX010000000
VERSION     DADQRX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.059"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..712)
                     /locus_tag="J8V14_001820"
     CDS             complement(56..712)
                     /locus_tag="J8V14_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBA0921921.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            905..1183
                     /locus_tag="J8V14_001821"
     CDS             905..1183
                     /locus_tag="J8V14_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921922.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="J8V14_001822"
     CDS             1202..2410
                     /locus_tag="J8V14_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0921923.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="J8V14_001823"
     CDS             complement(2558..2923)
                     /locus_tag="J8V14_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBA0921924.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="J8V14_001824"
     CDS             complement(3070..3843)
                     /locus_tag="J8V14_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA0921925.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="J8V14_001825"
     CDS             complement(4017..4877)
                     /locus_tag="J8V14_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA0921926.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="J8V14_001826"
     CDS             complement(4877..6295)
                     /locus_tag="J8V14_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0921927.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="J8V14_001827"
     CDS             complement(6352..6801)
                     /locus_tag="J8V14_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA0921928.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="J8V14_001828"
     CDS             complement(7246..7509)
                     /locus_tag="J8V14_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBA0921929.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /locus_tag="J8V14_001829"
     CDS             complement(7608..8903)
                     /locus_tag="J8V14_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBA0921930.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="J8V14_001830"
     CDS             complement(8900..10171)
                     /locus_tag="J8V14_001830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBA0921931.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="J8V14_001831"
     CDS             complement(10336..11205)
                     /locus_tag="J8V14_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBA0921932.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11497..13107)
                     /locus_tag="J8V14_001832"
     CDS             complement(11497..13107)
                     /locus_tag="J8V14_001832"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBA0921933.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="J8V14_001833"
     CDS             complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="J8V14_001833"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBA0921934.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15668..16060)
                     /locus_tag="J8V14_001834"
     CDS             complement(15668..16060)
                     /locus_tag="J8V14_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA0921935.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4639 a         3449 c         2766 g         5417 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    11341 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    11401 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    11461 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    11521 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    11581 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    11641 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    11701 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    11761 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    11821 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    11881 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    11941 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    12001 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    12061 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    12121 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    12181 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    12241 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    12301 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    12361 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    12421 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    12481 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    12541 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    12601 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    12661 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    12721 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    12781 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    12841 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    12901 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    12961 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    13021 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    13081 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    13141 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    13201 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    13261 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    13321 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    13381 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    13441 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    13501 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    13561 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    13621 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    13681 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    13741 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    13801 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    13861 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    13921 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    13981 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    14041 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    14101 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    14161 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    14221 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    14281 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    14341 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    14401 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    14461 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    14521 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    14581 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    14641 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    14701 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    14761 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    14821 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    14881 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    14941 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
    15001 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    15061 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    15121 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    15181 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    15241 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    15301 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    15361 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    15421 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    15481 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    15541 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    15601 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    15661 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    15721 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    15781 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    15841 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    15901 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    15961 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    16021 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    16081 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    16141 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    16201 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
    16261 gtgtttttat t
//
LOCUS       DADQRX010000059        16125 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.060, whole genome shotgun sequence.
ACCESSION   DADQRX010000059 DADQRX010000000
VERSION     DADQRX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.060"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..461)
                     /locus_tag="J8V14_001835"
     CDS             complement(<1..461)
                     /locus_tag="J8V14_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBA0921936.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLA"
     gene            complement(591..1301)
                     /gene="budA"
                     /locus_tag="J8V14_001836"
     CDS             complement(591..1301)
                     /gene="budA"
                     /locus_tag="J8V14_001836"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBA0921937.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1314..2978)
                     /gene="alsS"
                     /locus_tag="J8V14_001837"
     CDS             complement(1314..2978)
                     /gene="alsS"
                     /locus_tag="J8V14_001837"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBA0921938.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3183..3845)
                     /locus_tag="J8V14_001838"
     CDS             complement(3183..3845)
                     /locus_tag="J8V14_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBA0921939.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3855..4670)
                     /locus_tag="J8V14_001839"
     CDS             complement(3855..4670)
                     /locus_tag="J8V14_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0921940.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4932..5375)
                     /locus_tag="J8V14_001840"
     CDS             complement(4932..5375)
                     /locus_tag="J8V14_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBA0921941.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5509..5847)
                     /locus_tag="J8V14_001841"
     CDS             complement(5509..5847)
                     /locus_tag="J8V14_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0921942.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5835..6212)
                     /locus_tag="J8V14_001842"
     CDS             complement(5835..6212)
                     /locus_tag="J8V14_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA0921943.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6436..7629
                     /locus_tag="J8V14_001843"
     CDS             6436..7629
                     /locus_tag="J8V14_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0921944.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7795..8217)
                     /locus_tag="J8V14_001844"
     CDS             complement(7795..8217)
                     /locus_tag="J8V14_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBA0921945.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8612..8806
                     /locus_tag="J8V14_001845"
     CDS             8612..8806
                     /locus_tag="J8V14_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921946.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8803..9297
                     /locus_tag="J8V14_001846"
     CDS             8803..9297
                     /locus_tag="J8V14_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921947.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9459..10631)
                     /locus_tag="J8V14_001847"
     CDS             complement(9459..10631)
                     /locus_tag="J8V14_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBA0921948.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10837..11652)
                     /locus_tag="J8V14_001848"
     CDS             complement(10837..11652)
                     /locus_tag="J8V14_001848"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBA0921949.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11802..12248)
                     /locus_tag="J8V14_001849"
     CDS             complement(11802..12248)
                     /locus_tag="J8V14_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBA0921950.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12245..13249)
                     /locus_tag="J8V14_001850"
     CDS             complement(12245..13249)
                     /locus_tag="J8V14_001850"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBA0921951.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13289..13618)
                     /locus_tag="J8V14_001851"
     CDS             complement(13289..13618)
                     /locus_tag="J8V14_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBA0921952.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13744..16071)
                     /locus_tag="J8V14_001852"
     CDS             complement(13744..16071)
                     /locus_tag="J8V14_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBA0921953.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4604 a         3236 c         2861 g         5424 t
ORIGIN      
        1 tttgctaata aaagatctac atatttttct agaaatgctt gatcgatttc atcataccct
       61 ttcgttactc cgcaatcgat atctaaaaca ccgattaaat taccttcttt taccattggc
      121 actacgattt ctgattgtgc ggctgaatcg caggaaatgt aatttgcatg ggttttgaca
      181 tcatcaacga tcaatgtttc ttgtttttca gctgattctc cacaaactcc ttttcccatc
      241 ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta aaatcaattc attaccatcg
      301 aataaataat atcctgcaaa gacagtctca ggcaaagtct cattcaacaa tgcagatgaa
      361 tttgctaaat ttgctaacca gttatcttct gcttctaata atccttgttg ctgtaataat
      421 aaaatttcgt atgcatcttt tttctgtttt tctgtccaca tgataaaaac cgctcctttt
      481 tgatctatgg agaagattat agctcagttt ttttgtaaac gctaggtttc ctcctcaaaa
      541 aaacgagata cgtcaggtta atcaccttga acgcatctcg ctgtcttttt ctattctgct
      601 tgttggattt ctgctgctaa atcagcataa tcaatcgttt cgtgaacaaa agtcggatca
      661 tcaactggaa aatgctgtac aagggacgag atgcggctaa tctcaacttt tccttcagcc
      721 acttcaaaat ccattacatg tccgccaaat gtatgtgtat catcgataaa atgcaagtga
      781 aatcccgctg cagcaactcc ttggaataat tcaggcgtaa agaacccgac taatgttcct
      841 tggacattgc tttgttggaa ttctggttga gactctgaaa tttttgctag tcttctgtaa
      901 ggtttttctt gcttcggcat gatacggatg tgcatatttt tgaataatcc agttatcttc
      961 actgccaaaa ataggttttc cccggcttct cttactaata tgtctttttt caattgttct
     1021 ccatctgcag tttttgagac agaaaaacta gtatctggcg taaaatctgt aattgctgca
     1081 tagggggtaa gttcacttcc atctaattgg gcaactgagc catctgatct tccttgataa
     1141 gctacgccat ctaaaaagat gacttcacca tcaagcccat gcaatgttcc caatcccaga
     1201 gttccttttt ctaatatatg agcaatcgtt tctgttccat ccatcaagcc agccatcaaa
     1261 gctcctaatg tcccgtgctg gtataagatt ttttcagtca tgtcgacact tccttaatag
     1321 aattgatctg gtaagattgt ttctcccaat ttctcattat cacggtaatc aatcggaata
     1381 tcaataatta cgggtccttc tgtcgcaaat ccttcttcta aagctttttc taattcctct
     1441 acacttgtag ctcggattcc tttagcgcca aaagcttctg catatttcac aaaatcaact
     1501 ggaccaaagt ctacacctga cgcccgctga tatttcattt tttcttggaa ctctaccata
     1561 ttgtagtgtc catcattcca aatcaaatga acgatattca attttttacg taccgccgtt
     1621 tctaaatcct gggcagagaa taaaaatccc ccatcgccgg aaactgatac gatttgtgta
     1681 tttggacgca ccaatgcagc agaaatcgcc catggcaatg caacaccaag cgtttgcata
     1741 ccattactga ataacaaatg acgaggttca taacttctaa agtggcgtgc catccaaata
     1801 taatgactac ctacatctac ggtaacagtc atatcatctg ttacttttga ctgaagcgtg
     1861 ttaataactt ctaaaggatg caagatacct gcttctcctt ttgattgaac aatgtcccgc
     1921 tccgttaatt ctgcttgaag agatgctaaa tattcttttg catcttctga cacttgttga
     1981 ggctctaatg aacctgtcaa taaatcaaga gtggctgaga tatcaccgat caattctctt
     2041 tcaggctgca taaaaggatc gatttcagct ggcgcttcat cgatcacaat gatccgcgca
     2101 tctttttccg cattccagtt tcttgcttca tattcgattg gatcatagcc aatagcaata
     2161 actagatcac ttcgtttcaa aagcatatct cctggttggt ttctaaataa acccacacga
     2221 ccaaagaaat gcgcttctaa ttcacgtgag atcactccag ctgcttggaa tgtctctaca
     2281 acaggcaatg ctgtctttcc aactaattgc ctgatggcta acgtttcttt ttcacttgat
     2341 cctcgcatac ctactaatag taccggtagt tttgcttctc taatgcgttc agctaggtaa
     2401 cggatatcat ctgcagaagc agaacctaat ttaggatcgc tcaatggctt gatgacattc
     2461 ccttggacag gtgcatccac tacatcttgc gggatactga taaagctcgc gccttttttt
     2521 gaactcttag ccatacgata agcattggca atcacttctg aaattgtttc tggatcttgg
     2581 atttctgcac tgtattttgt gattggctgg aacagtgctg cattatccat gctttgatgg
     2641 gtcaacttca atagatcgct tcgttttact tgtccggcaa ttgctaaaac tgggtcacct
     2701 tctgctgtcg ctgttaccaa acctgtagct aagttactag caccaggacc acttgttgcg
     2761 ataactactc ctggctctcc tgtaatacgt ccgattgctt gtgccataaa tgcagcattt
     2821 tgttcgtgac gagtcacgat taattcagga ccttgatcct ctaattcatt aaatacacca
     2881 tcgatttttg cacctggtat accgaataca taatcaacat gatgattaat caaactttct
     2941 actacagatt tcgaaccttg tttcattttt tcgtccattg aacatcgtcc tttttatgct
     3001 ttatttttaa ttattagtta gaaataatca caaataattg atacagtttc taaacctgtt
     3061 tcattcaaaa gtatagcacg ttttatttca tttgtttagc catatgtttc aaaaaatgaa
     3121 aaagaatgac agtttttaca taaaaactgt cattcttaat tgaaagtttc aatttataaa
     3181 gtctacagat ccatagttct catcaattta cgattgttga agtaaatcaa accaaatacg
     3241 atagaaggac ctagtagaat acaccattgg ggctttacga aaaagaagag aaacaaactt
     3301 gtcaatagta aaaaatctag taaactcaca taccattttt tcaacgtata gtagaacgct
     3361 atccgtagaa cttgatttgg tttcgacgct ggatttctca cctgaaaata ccagtagttg
     3421 atgctaaagg aaactgataa acaagcaagt aaaacaaaca acggaatcaa ccattttcca
     3481 taagaaaaac gtataaaaaa gaaacaatca aaaatcatta tcacactaac tatccaagca
     3541 aatagccaat aaaaaaatcc ctttagccaa aattggcgaa acgtctttat gaacaaacgt
     3601 actggtgaaa aacttttttc ttttgctaat tcttctgtca tacctatcat cacgatgcaa
     3661 cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga cattccttcc atctatcgac
     3721 aaaaggaacg cagctgctat atacaagata ttgacacacc aaaaacaaag gctacttgcc
     3781 aatgattgat acaaccaata aaagaattcc atataaacat ttttgtcaaa acgctgctct
     3841 ttcataatca cctcctattc cttcattcct gatgaagcga tcccttctac aaaatatttt
     3901 tgcatcgcta aaaatataaa agcaacagga agaacagaaa caactgaggc agccatgatt
     3961 aatgcgtact ctgcatcaaa tagcccaata aacattttca accctaactg gattgtttta
     4021 ttttccgtag aagacaaata aatgaatggc cccatataat cgttccatgt attaacgaat
     4081 gtgatgatcg tcaaactggc aatcgccggt tttgtaagag gtaaaatgat cttgcgatag
     4141 attccccact ctgataagcc atcgatccgc gcactctcac ataaagaatc tgggatacta
     4201 ctgtagtatt gcttcaacaa gaacacacca aaagcattga acgcttgtaa tagcaccaac
     4261 gaccacaacg tatatgttaa acccactttt ctcatcataa tgaactgcgg gatcatataa
     4321 gactgccaag ggacagcaat cgtgccgata tatgctaaaa ataaaatatc gcgcccttta
     4381 aaatttagtt tagaaaaccc gtaagcagca aaactagaag taaacaactg aatcaacgtg
     4441 atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca ctaaaggaat acgagaccaa
     4501 ataaccagat aattctctaa atgccaagtt ttagggatcc attgaatcgg aatcgtgaac
     4561 acttcattat ttgctttgaa tgaagcagaa accatccata aaaaaggaat gatcgtcaat
     4621 aaagctaata caatcagcag aatcataccc aaccattgaa taaagggcat gttttttgaa
     4681 cgaatttttg ctttttttct ttcatttcga acacctccaa agttataatc ttcatttctt
     4741 tgtaatgatt acaatccaga acaacggtta ttatagctga ttcatttccg ttcttcttat
     4801 gtttgtaagc gcttatttaa caagttatgt aaatctcttt attttttttg atttaaaaat
     4861 gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt gtattgaaat ttcttcaatt
     4921 agagatactt attactgttc aaaagcttct ttgaaacggt gaatcaattc attgactaca
     4981 ggtgataaaa tccgattctt tttccagact agcatgcagt tcgtttcaat cattgggtca
     5041 agcggaacga atgtcactcg ctttgaaaga tgacttacag caccttctat gactaaagca
     5101 gcacctacct gattctcaat tagcgcaaat acattgaaaa tcaaattgta cgttcctgtg
     5161 atcagcaact gctccttttt tatgcctaac cagtcagcaa acattcgctg tatttctgga
     5221 cgctttgaga tcaaaagagg tacattcgca aagtcttctt ttgttatcac gtcttttttt
     5281 gctaaaacag aattttctaa aatgagcaat ccccattttt cctttcttgg taacaaaatc
     5341 gtttcgtatt tctctgtatc tactggatct agcaaaatcg ctaaatctaa caggcttttt
     5401 tctagtcact ccgtgatatc atcacttgtt ccgctgtgga tatgaaattg aacgctggga
     5461 taatcacgga tcaacttttc tagtaatagt gccaatgtat tcgaattatc agcttccaca
     5521 cagccgatcg taaatcggcc gctgaataat tgtttttttc tgttctcgaa ttcttgagtg
     5581 gtttgattcg ttaaatctaa aatttcttct gctcggcttt tttagaaaat aacctgcttc
     5641 tgtcaagatc attttgcggt tttcgcgtac aaacagttct gttcctagtt cctcttctaa
     5701 ctccttcagt tgtcggctta aggttggttg tgtaagatgt agaacttctg cggcttgaga
     5761 gattgttcct tcttctgcaa ttgtccaaaa atattttagt aagcgtaact ccatagcgat
     5821 gctccttgat ttttttactt gatacattcc attttatttc tagataacac acgatcatat
     5881 ttcttgattt ttccttgtaa acgttccaaa cactcttgca tttcagcaat ttctttcgtc
     5941 aaaagttcac tttcttcaat caaaagctta cgccgctcaa atacagttgc ttctccttcg
     6001 ctagataggc gaatatattc aatcaaagac tcgatcgata atccggcatt tcgcatacag
     6061 atcgtaaagt cgacccattt taggtcttct tctgtataat cacggattcc gccttttgtc
     6121 cgattaactg gtggaatcaa cccgattcgt tcataatagc gcaatgtatc ggccgaaatg
     6181 cccttttctt cactgacttg tttgatattc atctgatcac tctttctctt aatttcttgc
     6241 cactttactt atgtatttta tttattctac aactttaagt gtactccaag tcaatactca
     6301 tttctattaa aactaagctg gattatctat atcggttgtt tgtgtattat ttcaaataaa
     6361 aaagtcttgt ttttttattc ttttttggat atacttttaa caatttaggg aaattttagg
     6421 aaaaggggta taaaaatgac aacgtatcag cgaaaggtat tattatcgac atcggcggga
     6481 attgctttag agaatatgga tattatgttt ttagcttttt cattatcttc aatgattact
     6541 tcttttcata ttagtggtac gcaagcgggt ttcatttcaa cgatcactaa tttggggatg
     6601 ctcattggag gtattttttt tggcatcata gctgataaat atggacgtgt gaaagtattt
     6661 tctcaaacgg tccttctgtt ttcgattgct tctttattga tgtattttgc ttcaaacatc
     6721 tacttggttt atctctttcg ctttatcgca ggtgtaggag cgggaggaga atacggggcg
     6781 tgtatgtcct tgatttccga aagtttttca aaaaaacaaa ttggacgagc ttcttcggtt
     6841 gctggtattg gtgcgcaggt cggtgcggct ttggctgcga ttttagctgc tgtgatcatt
     6901 ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac tgccggtgtt gatggtcttg
     6961 ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat ctgtacaatc tgccgaaaag
     7021 aaaaccaaac ttagcagact gtttgaatca aaacatcttg cttggcaaac atgtggcctt
     7081 agtatcatgg taactgtcca aattgccggc tattttgggt taatgaattg gctgccttca
     7141 attatgcaaa atcgattagg gctttcggtt tctgggtctt cattgtggat ggtcagtact
     7201 attttaggca tgtctttggg gatgatgact tttggcataa tcatggataa attggggcct
     7261 aaactagcct tttctttgtt tttaatttgt tcatccttat ctgtctatct acttgtgtta
     7321 gcccatagcc agtggagttt ggtagcagcc gctgtgatag tcggctactt tatcaatggc
     7381 atgtatggtg gatatggcgc agtgattagt agtttgtatc caacagaaat ccgcgcgacg
     7441 gcgaataatt tcattatgaa ccttggacga gctgttggtg gtttttcttc tatcgtcatc
     7501 ggtttcttga tggatcatta tagtttaact gccgtaatct tgtttttgag cggtatttac
     7561 ctagtcagtc tgctggtctt gacaactctt actggtgtta cagcattaag ggaaagcttg
     7621 cagcattgat agttgagtct gtaaatgaca agaaagaggt gtggcaaaag cttgcatctg
     7681 cggtaataag accagccaaa tcaaaaacat gagacactgt ttccgtttgg ctggtcgtat
     7741 tacttgatgc agggcagctt tttcaactgc taatttcctt ctatctggcg gcgactattc
     7801 tttgacgtcc gtcatttcaa agtactttac taataactta gcataacgaa aaactgtgga
     7861 tacactcgta tattgagcgt ctgcataatc ttccaaattt ttctcaggat gttgatacat
     7921 gaattcaatc aaatgataat tatatgattt tctgtagaaa tgaagataaa taatgtagat
     7981 tgggaagttc tcctgctttt ccatagaaaa gcgccgatta ttctcattct tcaaaatgac
     8041 cttatcagga tatttttgga acaattcttc taaatctgtt agataattca agatagactt
     8101 acttgatacc ttcaattcgt tttgtagttc ttctattgaa caccaacgtt ctgtttcaag
     8161 cagtaaatga atcattttaa ttttcttttg agaaactttt gttaaaaaag tctgcatatt
     8221 ttctcctcct gtatctactt tactatacta catacaagaa aaaaaacgga tacaaatatc
     8281 atattgtcac accgtcaata gtgcataaac ctgttattat ttctcttttt ttcctaagta
     8341 ttctattgat atgaatcatt taatcgttta ttatataaat gtcaataatc taataataaa
     8401 ataacgtaca tattttatta ttgcatactt aataacagaa ctatagcaaa aaatcccgtg
     8461 tatcaacacg ggattagatt aagtttcctc aatcactatt cataacacaa ttattataac
     8521 aagtaagtaa acaattgaaa aggacattta ttgaaaacgg ttgtttttta cttaaggagg
     8581 aaatttagcg aaaaaattag ttgcattgtt tttggtcgca ggaagtttgt tgagtgggtg
     8641 tggtgctact acgtctacta ctcaaagcac aagttcagat caggcttcca gcagcagtac
     8701 cacgatctcc gaaagtacgg cagattcatc gaaagaacaa gaacaaattg aaaagaaaaa
     8761 agaagagaaa caaaaagcgg aaaaagctgc actagaaaaa aagtgacaga agcggaatct
     8821 gctttgtcac aagctgaagc aaatcctaca aaagaaaact atgcaactgc ttcagcagct
     8881 atccaagcgc tacctgaaaa caaacaagag ttgacaagcc gtcttgctgc aattgattcc
     8941 acaatcaaag caaaagaagc agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa
     9001 caagcagaac aagcgcaaca agaagcaatc gcacagcaac aagcagaaca ggcgaggcaa
     9061 gaagcaatcg cacagcaaca agcagaacag gcgcgacagc aatcagcagc tgaacaggcc
     9121 tctcagcagg cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca
     9181 ccgaccggtt caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact
     9241 ttaagcgaag ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc
     9301 aaaagttata ctataataat aaaattcttc tgaaacataa aaaaatagaa gatggcgttc
     9361 cagatagtaa accaaaaata acagtttaca cgtccatttc gtcagcttct ctcttttttc
     9421 atttagtctg ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag
     9481 tacacctctt tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt
     9541 ttcgaatgtt tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag
     9601 ttcctgcctt ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat
     9661 ggaacttttt ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt
     9721 ccttcgattg gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta
     9781 gcgatggcca aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt
     9841 agtataccga taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc
     9901 gctttttttg cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt
     9961 gtattgacca tataaggagt acaggtaagc aaagtcacga gatctctgcc tttttggata
    10021 cccaaagaat ctacttcatc aggtaagacc acttggattt tttctacttg atacgccaat
    10081 gtctttccat tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta
    10141 aataattttg cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg
    10201 ccagtcggat aagaactttc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt
    10261 gtctgatcga atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata
    10321 tgttccataa aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg
    10381 ttgaaactgc ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc
    10441 attttttcct gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa
    10501 tggatgatca tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa
    10561 aagatcccga tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg
    10621 cttttattca tcatgtccac ctattttcta atatgtatat gaagaggttg tgaaatcagc
    10681 gccttgacct gagtattagc gaagcaaacg gcaaaatagt tcctcctatt ttgccgtttg
    10741 ttgagcgaat accaagagac cgaccacttg tcacaacctt tctccccatt tgaatcacta
    10801 tcgtttattt atcgtatgat ttttgtttcc aatagtttaa gcttctgttt cttgatggcg
    10861 ttttttgaaa tagatcactg ctcctccaac taaagcagta cctaccaaga tgaatacgat
    10921 gatccccata ccaccagttg aaggcaagat tcctttaggt tggttatcga tcgttgtttc
    10981 atcagaagtt gttggatcat catctactgt aaattcagtg tacgcagctt cattcttagg
    11041 taatgcgtag ccttctggtg cttttgtttc ttgaaggcag tatgcccctt gtttcagccc
    11101 ttcgattgaa aatttccctt ctgaatcaga aatcaatttc acttcttccc agtcagaacc
    11161 tgtgacttca tctgaccaag catatgggta ttctcctgag gcaaggtttg ctattttttt
    11221 cattaattta acagttggat cttttgctct tagaatagaa aattctgcat cggctaattt
    11281 ttcttgcgtg ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt ggacattttc
    11341 tacagtaatc gtgttatccg tagttgtatt ttcccgattg actacaccgt ttggattccc
    11401 aagattggct tcatctgcgc catggatgat accggttgtt tgttgcaatt ttccgttttt
    11461 tactataaaa gtagtttctt tttgtggatt ggttaattga taaccatctg gtgcttttgt
    11521 ttcaactaaa gtataattgc cgtctattaa tccattgaca ctgatggatc cttcagtatc
    11581 agttgtgaat gtcaacgctg actcagcagg atcatagaga gcgacaccag tgttttcatc
    11641 aaaaccggtc acgaattgat tctctgaatt tttgatctgg aattgcgccc ctgctaactt
    11701 atctacccct gaaaagttcc cagctttctg gattttgata ttgctttctt tgatgacatt
    11761 tttagcatat aggtgaatcg tgtctaaagg tttgtcaggg tctagggtaa gcttaacacg
    11821 atatttggcg acgtttcgac accgggcttt ggtgtttcta caaagacgta tacagcatct
    11881 ttcccatcag atttacctgg aagagtcagg ttagctaatc cttcaccaga agtcgttaca
    11941 cttgcgaagg gtgtagcgtc tgctggaact gctgtgtctt tacttggata gtacgtagca
    12001 gtgatgtcat atacgttgaa ttcaacgccg ttcaacggtt cgctattcgg aaatgtcaat
    12061 tccgttccgt tgtttatttg attttctggt gctgaagtga atttcaattt atgcaaatta
    12121 accgtaacat tttctggtgt aggtgatacg gctgcagaaa cctctgcagt tcctaggccg
    12181 agaacgaaag gaagcagcat gatagcagtt cctaaaaacg tccatatttt tgttttgatg
    12241 tttttcattt tatttccctc ctattgtttc ctttttgttg ttttttataa aaccaagctg
    12301 tccctgttga aaggactagc aataacccta aaattgagaa acctccaagc atctcttcgc
    12361 ttgtttttgg aaagcttcct ccattttttt catttgatgg accatttgtc tgcatctgaa
    12421 ctggcgtttt tccattattc gtcggatttg cttttggttc gttgatgatt tccaatggta
    12481 taggatgaag cggatcataa ctgccttttt caacttcaaa gagaatggtt gattgactca
    12541 atatatatcc tttaggcggt ttaatctcca ctagttcgta acttccaaaa gacaatccat
    12601 ggacagaaaa tgttccatct ttactggaag taaaaatcgt tgctcttgaa tcatctttgg
    12661 ctacccaatg atttatcccg ttttcttgcg ctaagtattg gtcatttttt ttgactagaa
    12721 attgagcatc tgctagtttt tgtttcgttt gccgatccac tttgacaaat tgtttgcctc
    12781 ctgtttggat ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt
    12841 tatcaaatgt cgttttttca ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt
    12901 tttttccagc aaacgaacta agatcttttc cagaatctga taagacaaaa ccatgtttta
    12961 ttttttgtgt gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg
    13021 gttctagcca taactgagga tcagctgtat ctttgatcgt ataggttcct atctcgttga
    13081 tgttctcagg aattgttacg gtgatgagat aagggacttt atccccatct gcaaaatctg
    13141 tctgcttatt ctctactgtt tttgttaatg tgagtgcagc aggtattttt tgttcgctct
    13201 ttggatacaa gtggatggtc gtcaatttat gattagatga atctgtcaca ggaagcacaa
    13261 cgacaaatgg tgctgatttt tcttttactt aatctgaagt ctttatttcc gtaaatcggt
    13321 aaacagcgtc acgaccttgc tcatctttgt ccgatgcact aaaagaagca atgccctctt
    13381 cctcgtctac tgtttttgtc acattttctg ccagtatttt ttcactatca cttttttgtg
    13441 ctagttttct ttgtgcgtct tccacactac ttccttctga ccgcagcttg taaaattcat
    13501 ccgagacatc atatatagcg aaagtaacgc cgttcaaccc ttttactcct tgaaagttat
    13561 ccgggatatt tattccgtcg ttttgactga tacttggcat cttatcgctt tcgaacattc
    13621 gattatgcaa agtaaatgta ataggtgatt ctctagctga cacagtcgga gaacctataa
    13681 aaaagaccag taggataaaa aagagaacag caaatatatc cacacgtaca ttttttgttt
    13741 ttttcatcgc tgttcacctg cttttttctt acgatggata aaataaaaga agccagaaaa
    13801 aataaagatc agtcctaaaa agacaaaggg cagtagacct atcccacctg tcgaaggaag
    13861 caagagagtc ggattgatcg tcaacttagc ggtatctgtt gcgataatcg tataattatt
    13921 gtttacactt ccttcgccta aactaatcgt tactatttgt gatccaatat ctgtccggct
    13981 gttaactgtt agggtataat ccaatgattc gcctaagaca acaccggtaa cgtggagatt
    14041 tccaaccgaa atcgagtgac tttgggcatc ataattaaag gcagcgtcat cgacagtaat
    14101 cactgcgtta gctttataga tggtataagt tcctgatgtg ttggctatat cagtaaatga
    14161 caacttggga tttgctgctt tgactttgtc tattccgcta ttcgttagtt taattgtata
    14221 gctgccagca ttaaccatat tttttgctga ttgtgcatct gctgtgtaat ccatactgct
    14281 aagtgccact tgtactggag taagcgaact agtgacaccc tctgctagct tgacatggat
    14341 tgacgggaca cctgcttttc catcatatgt cttactgcca ctgagctcta ccgcaccgct
    14401 gatttgctta atcgtataga catcagatgt ctgcacgata gtaaataaat agtttgttct
    14461 ggcagctagt ttagctagtc cgctttttgt caattgccaa gacgtatagg ttccaatttc
    14521 agtaggcgca gttacttcaa ctcccttttc attcatcgct ttgaaatcag ttggattcca
    14581 tgtgatttga tcaatactga gatcattgtt taaaagggat gttgattctg ttttatcatt
    14641 aaaggcatat ttaacgatcg gtacaacaga aagaccatca tagtacttac tgccatttgc
    14701 attgattgat actgttgcat ttgtcttata atataggatg acttcagaaa tctgttttcc
    14761 atttgcgtcc tttacgatta catttccatt tgtatctaaa ctagctaaaa cactcattgc
    14821 tgattggttc tttgtttcta tcgctgaaac agctgtaaag gtatatccgt tgatagtagg
    14881 cgtattagtc gtcaggtctt gtgcagcact tccgtaagta agattcatta ttttagaagg
    14941 agcaatcatc tgtccaaaat agtcttgata cttaatagtg attggtgttt gtttgcctgt
    15001 ataggtcacc gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa
    15061 agaatagccc gttactgctg ttggagtgat aaccactttg tcacccacat ggattccaga
    15121 taatgtagtc ccgtacgttc cgatttttgc tgctccacca ttgactgtat aagaaaaagt
    15181 tcctgtccga ttcgcattga cctgaaccgt tgctgcttga taggttggaa aatttgaaac
    15241 atttccctta ccatcaacga tctgcccatt tttgatcgag taccccgtga atggaatatc
    15301 atttgctaac tgtataccga ttgaaaaagt actcatacct cctccggcag gaagccctcc
    15361 ccatgttgag ggtgtaaaag aataactttt atccgcattg ggtgtaactg aatcgatcgt
    15421 atttccagcg gtagccaccc ccatatagat actttttggg aatgtgatag tagtaccatt
    15481 tactgtggag ccaataaaca gattatggat atctggacgg ttatcaataa aagcgattgc
    15541 tttggttacg ccttgtaacg cagttttatc cgcactcatc gatccgttaa ccgcaacaat
    15601 gactccatct ttggcatata tcactgtata tccatctggt atggtatgtc cgttgaaatt
    15661 cgcagtggcc gcaacagtac taaatgtctg gcttctacct gtttctgaat aagctttctc
    15721 ccctgctgct tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga
    15781 tgtggttgag gttgtctcgt tgttgctaga ctgattggtg ctttctatgg actgtatttc
    15841 agaatagggt ggactatttg tctcctctgt cgtttggtta gcaaaatttg acgtttctgt
    15901 ataaacgttt acataaatac ttgataagct ccctaagaaa gtaaaaatca agatgatcga
    15961 aatcataatg ggatgtaagt attttctttt agatacagct ttgagtctag aaacaaaatg
    16021 tttttgacat atacgttgga ttttattttg agttttctta atcattttca tccctcctct
    16081 caataaaaaa tcatcaaata atcctttaca cgttattcta aattt
//
LOCUS       DADQRX010000060        16062 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.061, whole genome shotgun sequence.
ACCESSION   DADQRX010000060 DADQRX010000000
VERSION     DADQRX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.061"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..684)
                     /locus_tag="J8V14_001853"
     CDS             complement(37..684)
                     /locus_tag="J8V14_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0921954.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="J8V14_001854"
     CDS             complement(692..1378)
                     /locus_tag="J8V14_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0921955.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="J8V14_001855"
     CDS             complement(1384..2457)
                     /locus_tag="J8V14_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBA0921956.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="J8V14_001856"
     CDS             2679..3353
                     /locus_tag="J8V14_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0921957.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="J8V14_001857"
     CDS             3358..4698
                     /locus_tag="J8V14_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0921958.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="J8V14_001858"
     CDS             complement(4847..5833)
                     /locus_tag="J8V14_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBA0921959.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            6557..7588
                     /locus_tag="J8V14_001859"
     CDS             6557..7588
                     /locus_tag="J8V14_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA0921960.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            7697..9229
                     /locus_tag="J8V14_001860"
     CDS             7697..9229
                     /locus_tag="J8V14_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBA0921961.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            9368..10180
                     /locus_tag="J8V14_001861"
     CDS             9368..10180
                     /locus_tag="J8V14_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBA0921962.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            10170..10655
                     /locus_tag="J8V14_001862"
     CDS             10170..10655
                     /locus_tag="J8V14_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA0921963.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            10678..11223
                     /locus_tag="J8V14_001863"
     CDS             10678..11223
                     /locus_tag="J8V14_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBA0921964.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11296..12939)
                     /locus_tag="J8V14_001864"
     CDS             complement(11296..12939)
                     /locus_tag="J8V14_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBA0921965.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(13068..15296)
                     /locus_tag="J8V14_001865"
     CDS             complement(13068..15296)
                     /locus_tag="J8V14_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HBA0921966.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(15329..15808)
                     /locus_tag="J8V14_001866"
     CDS             complement(15329..15808)
                     /locus_tag="J8V14_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921967.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         4741 a         3062 c         2907 g         5352 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat
     6001 gtcatttgtt tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat
     6061 ggtttaattt atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc
     6121 aatataattg ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt
     6181 tctaatactt ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa
     6241 tggagagcca atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc
     6301 ctcctgccat tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa
     6361 aaagaggata aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa
     6421 tgtatcttct tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat
     6481 caagacaaac tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta
     6541 ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca
     6601 acgtacacac ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct
     6661 ttcattaggc agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag
     6721 aaaagtattt tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta
     6781 tcagaatttc ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca
     6841 agaaacggag caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt
     6901 aaatcatttt ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt
     6961 gggtacaagt ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt
     7021 caaagatttt gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag
     7081 ctttgaagaa aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg
     7141 attaaagatc catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat
     7201 tcttaatctg aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat
     7261 gccatttctg gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt
     7321 ttttcgttcc tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa
     7381 aattacagta tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc
     7441 agaactatat ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc
     7501 atcccattct tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc
     7561 ggcattcgtt cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac
     7621 tatgttatta tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa
     7681 agataatgga gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa
     7741 ttttcaagaa gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc
     7801 agctttccca gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa
     7861 acgggcttat caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa
     7921 gagttccgct tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc
     7981 tgtcatgact tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga
     8041 agaccctttc attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg
     8101 aacaaaatca aaacagattc ctattcttta tttaatggaa caacctgctt tgtctgtttc
     8161 tcaatcattt ttagacaaaa atgaaatttc ttatatgacc catacttctg gtacaactgg
     8221 tatccctaaa cttatttgtc attctgctca ttctatggga tggcggacga aatggcaaaa
     8281 aacagttttc acaaaaattt cggaaaagaa attaatcgct tttcacatct ctccagtgca
     8341 ttccagattc aatattggta tctcttcttt gatgtccatg ggatttccta tgatgccttt
     8401 agcaaacgca caaagttcaa aagttgttca tatgttggaa gctcaccggc caattgcact
     8461 agagacccat ccgaataact ttgttcagtg gcgttttact gctaaggagc atccagaagc
     8521 tttttcgggt atccgatact atcactccac ttttgatgct ataaacaacc agacaatgaa
     8581 gacatttttg cgaacgtcga tggcacagga tgcggtattt cttcaagtat atggccaaag
     8641 tgaatgcgga ccaatgattc ttaaggctca tacactagag agtttgaaaa caagtgatgc
     8701 aagagatatg ggcgttggac tggaagacat gacaagcgct cggattacag attcagttgg
     8761 gaatgtccta cctgaaaaca cagatggtca tatacaatta ctgtctaaag gacgggcttt
     8821 gacttattac aaggaagatg cacggttcca ggaaaatgtt tatggcgatt ggtgggatag
     8881 cggagattac ggttttatgg ataagcatgg ccacctattt ttgaaagatc ggcaagtgga
     8941 tttgatcgaa acgatcaata gtacattagc tattgaagac tttttattag attcgttaga
     9001 ttttttggca gaagtagtga ttgtaaggga taaaaatcat tctccacaac cgattattgc
     9061 tttagctcct gataaagaaa tggattggaa tcgatggtgg gagcaagtcc atgaacttcc
     9121 tcgcttgaac gtccctatca tccgtgattt tgatgcaatc ccgcgaacag ctacaatgaa
     9181 agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa aagtcctaac tatttaagga
     9241 aagaaagatc cattttggtc tttcttttct ttttgttttt ttaaattgga tgagataaaa
     9301 atatgccaat tggctgtttt tcttttttga aaatttcgtt atactcttat tcatggagga
     9361 aaatgttatg aaaagaatct tgacaattgg cggttctgat ccatttgctg gtggaggaat
     9421 ccaaacagat ttaaaaactt ttgagaattt tggtctattc ggactaagcg ccttgacttc
     9481 tataggggca ctagatatta atgagcgatt tatgctggaa tctatatctt tggaattgct
     9541 taaacgacag ttagtttcga ttgataaaat gacttcatta gatggtatca aaattgggtt
     9601 attgaattca agtgaagcaa tcatggttgt tcgagacttc ataaaaacaa aacaaaatat
     9661 cccaatcatt ttagaccctg ttttggcatt caaagaaact gaagctgtga tcaatcaatt
     9721 gtatatttcg cagctcatcg aagaactttt tccacttgtt acgatgatta cgcctaattt
     9781 ggcagaagct gctttacttt ctggtcaagc tcttccctct tcgttaacag aaatgatcca
     9841 tctcgctaaa gaactcactg cttcaggaac aaaaaacatt gtgatcaaag gcggcacaag
     9901 gatggctgga gatgaagctg tggatcttct ctattcaatt gatcattatg aggtattttc
     9961 tcataaaaaa atcgacacta caacagtaaa cggggccggt tgtgcctttt cttctgcaat
    10021 aacagcgaat cttgtgttag gggcagattt taccaaagca gtagcagaaa gtaaaaaatt
    10081 tgttgcccat tgtatttcta atggcgtgct gatgaacgat cataccggaa gtgtctggtc
    10141 tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa gcaattaacg atctatgcta
    10201 tgctgattgc gttgacagtt gccctttcat taacaatttt gatccctgta ccggctacga
    10261 atggatttgt gacattatgc gaggccggga tctataccgc tgcttttctt tatggtccaa
    10321 cgggtggatt agttgttggt gcttccagtg gtctattgat cgatttgatt tcaggatatc
    10381 cgcagtgggc agttttttca ttagtgatcc acgggttaca aggatatgtt gctggttctt
    10441 tcagcaaatc ttctactcgt atgtggggat tagggctttt attaggaagc ttagttatga
    10501 tcatcggcta tggtatcgcc ggttggattt tatatgattg gccggctgga gtggcttcta
    10561 taccaggaaa catcttccaa aatctgattg gaatgggact tgcctttcct ataactgcaa
    10621 gtcttcaacg tattcaagca cggaagcaat attaattgaa aaagaaggtt ttagaaaatg
    10681 aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa tagttaatga catattggct
    10741 gaggcacact tgaaaaaagg tgatcttttt gttttaggat gcagcacaag cgaagtcgtt
    10801 ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc aatggatcat ccgtacgtta
    10861 aaagagttgt tggatcctaa agaaattgcg ctagctgtcc aaggttgtga acatttgaac
    10921 cgagcattag tagtagaacg aacagtggca gaagcaaaaa atttcgaaat tgtctctgtt
    10981 gttcctgcac tacatgctgg aggtgcttgt tctgttgctg cttttgacca gttcaatgat
    11041 cctgtcgaag tggaacacgt agttggacaa gccggtatcg atatcggtga cacatcaatc
    11101 ggtatgcata tcaagcatgt ccaagtacct gtcagacctc gtttgaaaac ccttggacaa
    11161 gctcacgtga cagcccttcg ctctcgtcct aaatatatcg gtggtcctag agcgaattat
    11221 tgaaatgagt aattgacaga gcaatctgaa aaacttcctc gcaaagtcca actaatgaac
    11281 tttaacgagg aagttttact tgtttgtttt atataaccga gcttcataag gtgctagtat
    11341 gcctttagct tcaaattctc cgctagagga atttgacaag agtaatgtcc aggactgatc
    11401 tgcttctttt ggaagttcat agtcagcaag tgattctgtt aagtttacca taataagata
    11461 ttgttcatct tctaaatatc tttcatagac ataaagttgt ggatgttcat gaagatattc
    11521 tttataagaa ccgtatatca gacctttatt ggtttgtcta atgcggatca tgttcttata
    11581 gaattgcagg accgactctg gttcatattc ttgttgttct acattgatcg ttttggtatt
    11641 tggattagtg atcagccaag gttcatgtgt agaaaaacca gcatattgtt ccgaagtcca
    11701 ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga atgatatcca atgcttcttc
    11761 tcttgtctta ccttccgcaa gcagcctttt ataaagtctt ttcgaatcta ctgcatcaac
    11821 ttgatcgatc gattcaaagg gcatattcgt cattccgatt tcctgtcctt gataaataaa
    11881 tggcgtacct tgcaaaaaga aatacatcat agcgattgct tttgcagaca tagcccaaaa
    11941 atcgggacga gtatctccaa acactgaaac ggcacgagga acatcatggt tttccatata
    12001 taaagcgttc catccgatac catccaatga agtttgccaa gcgcttaatg catgtttcaa
    12061 tttaaggaca tctaattgcc cttctttttc ttgctgccag atatggatat gatcaaattc
    12121 gaatatcata tcgaaataac cgtctttccc tacccattgt ggtccttctt ctgcagtgac
    12181 accacttgct tctccgacag tcatgatatc gtactcttca aaaacatgtt tcaaatcggt
    12241 caaatgttct tcgattcctg agacattttg aaagggtgag aaagggttct ctgtggcttt
    12301 tactgaatat tcatcttttt ttacatgaga aatcgcatct aaacgaaatc catctatccc
    12361 tttatccaac caccatcgga tcatattgaa tagttcttct ttcaatttct cgctttccca
    12421 atttaagtct ggttgttctt ttgcaaaaac atgcagataa tactgtccag tttcatgaga
    12481 atactcccaa gcagaaccgc cgaaaatcga tgtccaatta ttcggttctt tgccatctac
    12541 cccgtcgacc caaatgtaat aatcgcgata cgggctttct ttagattttt tcgattcttc
    12601 aaaccattga tgctgatccg aactatggtt gatgacaaga tccataatca ctttgatccc
    12661 taattcatgt gcttctttta gcaataaatc aaaatcttcc atcgttccga atctctcttc
    12721 tatatcttga tagtcactta cgtcataacc attatcgaca aatggagatt gatagatcgg
    12781 attgatccag ataaagccga taccaagatc tttcaaataa ttaagttttt cacggatccc
    12841 attgatgtct ccttgaccat cgccattgct atctttgaaa ctagcaggat agatttgata
    12901 gcctactgct tctttccacc atttttgttc ttgtatcata tcgtttcact cctttaacgg
    12961 tccctcttga ttataaccga ttctatttta gaagtatcct tgttaccggt aactagaaac
    13021 gaaaagctca acgaatctgc taagtgcgtt cgttgagctt tattttatca tagatagcgg
    13081 gcaatcagtt gaatcatttg cttgggaaat tcattcaagt cagtaatatc tacaaaacga
    13141 ctttcaccgt agatcgactt gatttcttct ttgtcttggc caatagctgc ggagacaaat
    13201 aggattcctt gacgttcata ttctttcaaa acagactgga tatcttcttt tgcttttttg
    13261 cctgtataat ccggcaacgc ttttggctga ccgtcactaa tattaagcaa cagtttggtt
    13321 gttgcggatt gtctgtttaa tttttctgct gctaaacgaa gtgcagcacc atcacgattg
    13381 ttttgccttg gtttcatagt gactaatttt tcatttaacc attgaaatcc gtcttcaaat
    13441 tctttataag aaaacaaaga agtcttttct cttgacgaac ggtctgctgt atctccgtaa
    13501 atcaacaatg ggatatcgac tttcttagaa aattcggcaa tggcaatcgc tgcttttttt
    13561 gccgcttcga tccgatcatc ccgtatcatt gacccagatt catcgatccg tacagctacc
    13621 gctaaagaag gttgctcatg tggcgggttc tttttgtcaa aatttctcaa atcaccatat
    13681 gctactttgc ttgcattgaa ccgctgacct tcgtattttc ctttttggta cgtctctgtt
    13741 tgttcattat ccagtaactc taaaatttgc cggctaagtg attctactat tggcattact
    13801 tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga aagctggtcg gtgaacgatg
    13861 agaccttctt tttcatgaat cgtttccttc atgatttgtc gcgcttcttt attcagttgc
    13921 ttcttgattt ccttatcatg tgcttccatg tcagaagcag acatttcttg agaatcatct
    13981 cctgtttgtt gttctttttg attttttcct tcgatatcgc tactgcgttc tttttgcatt
    14041 tgaggaggaa tctcgttttt ttctattgtt tcttcttgtt tctcttctcc ctcatttggt
    14101 tcttcagcag acaacgattt cttttcatct aagcgcagaa tttcttcagc attttcagtg
    14161 atgtttcgtt catttgcttc ttcggcgcta atggttcttt cttttgtatg ctcagattga
    14221 ggatgacccc atttttcttt tctcaatatg tcctgaagtt ccagtagcaa tccactgtca
    14281 gacaaagctt gttcaagaat tgccaattca tctgaatcag tggataattt atacaaaact
    14341 tttggataaa tcgactggag taaatcttgt ccttgcttat aggtatttgc ccaataaaaa
    14401 taagaacgca ttcctgctac acctttgatg gcgttagctt ttgcagtttc atccaacaaa
    14461 cggataatct ctgccatctt taagagaagt tcttgttttt ggatcatagt tttcgccatc
    14521 gcacgttctg ccatgacttc taaggcagga agatccattt tttccgcatg ttgcatccga
    14581 tctcttaaag attcatttaa tgggcgattt ccttggtaat ttcgatttgt agtgatcaca
    14641 atgacgcaat ctggatggcg acgaatgatt cctgtcggta aattcaacat cccatttggt
    14701 tcgagcgctg agttaagtgc cacaagaaca gaggcatctc ggatgacagt cggctcttga
    14761 atctctaata aatagccttt ttcaatcgct cgaacgattt cagaagggta atagctgtaa
    14821 tggattgcat ctggataatc agcttcgatc ctttgaacta gctctgctaa tttttcttta
    14881 gcagaaaaag gatcaatgcc gtaatggttc tggatcagtg tcagtacagc ttcaagtgta
    14941 tctgtttggt aaatagcttc tagtaattct tggtcttgtt ccttattaga atctactaca
    15001 ggtaataagg ctccgaaaac atctgatttg tccatatctg caaaacaggt cacttttgta
    15061 tagggaagtt gcagatcagc agatagtgct ttggctaatt gtgtcttccc cgacccggca
    15121 tctccttcca aaagtacgtt actgattttc atttcaggat cttgccaatt ccctttgatt
    15181 tcggaggcga tccgccattc ttctgcactt gtccgatggc ttttcggttt cttccagatc
    15241 atttgctttt ctagccgtga aaaggttcgg ttttctgtta gtgagaaatt ctccatgctt
    15301 ctcttcccct tttgatcatc tctgtctatt aatatcctag ctcatctaaa agacgtttta
    15361 aaatacggaa tcgttcccca atcagttctc cgtctttttg tagtgtatcg taatacaaaa
    15421 tgatgtcttc atcgatcttt ggatttttgg tatctacttc taccgtcatt cggttccctt
    15481 gtaatccaat gatgtctacg actggatttt ctggatcata aaatttttca taacgataag
    15541 catcttggaa gtataaactt ccttgcgcag ctaagatcgc taaatctaaa atacgtgtga
    15601 ttggtaattc ttccgactgt cgtgaccatt tttctccagt atgtcgccat accttgcctg
    15661 agatatcgac ttttccacga tcattccact gagcaaggcc cagtgaaagc ccttttgcat
    15721 ccatatttaa tgcatttctt ccatcaactt tatcatagtc ttctacaacg attactggtt
    15781 tatgttttaa atttgttggt atttccatta ttttaactcc tcatcctttt atttttagta
    15841 aataagtaaa ttagtagttt actcatttac taaactatat gcgacattat agaggattga
    15901 agtgaggatt gcaaagaaaa agagcttcat tgatagaaat aaaaacacca tctcgttctt
    15961 ttacacgctc attagtaaca tgtagaaagt ccaagatgat gttggaatat tttggctcta
    16021 tactttttga tgttggtgaa cttcaccaac atcttttttg ta
//
LOCUS       DADQRX010000061        15943 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.062, whole genome shotgun sequence.
ACCESSION   DADQRX010000061 DADQRX010000000
VERSION     DADQRX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.062"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="J8V14_001867"
     CDS             226..894
                     /locus_tag="J8V14_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBA0921968.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="J8V14_001868"
     CDS             complement(1094..1324)
                     /locus_tag="J8V14_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921969.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="J8V14_001869"
     CDS             complement(1451..2833)
                     /locus_tag="J8V14_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBA0921970.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="J8V14_001870"
     CDS             complement(2989..4176)
                     /locus_tag="J8V14_001870"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBA0921971.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="J8V14_001871"
     CDS             complement(4183..5214)
                     /locus_tag="J8V14_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBA0921972.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="J8V14_001872"
     CDS             complement(5230..6684)
                     /locus_tag="J8V14_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0921973.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="J8V14_001873"
     CDS             6859..7764
                     /locus_tag="J8V14_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA0921974.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="J8V14_001874"
     CDS             7855..8532
                     /locus_tag="J8V14_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBA0921975.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8813..9709
                     /locus_tag="J8V14_001875"
     CDS             8813..9709
                     /locus_tag="J8V14_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBA0921976.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="J8V14_001876"
     CDS             complement(9764..11134)
                     /locus_tag="J8V14_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0921977.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="J8V14_001877"
     CDS             11372..12118
                     /locus_tag="J8V14_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBA0921978.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="J8V14_001878"
     CDS             12115..12954
                     /locus_tag="J8V14_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBA0921979.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="J8V14_001879"
     CDS             12955..13905
                     /locus_tag="J8V14_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBA0921980.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="J8V14_001880"
     CDS             14046..14798
                     /locus_tag="J8V14_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921981.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15943
                     /gene="queG"
                     /locus_tag="J8V14_001881"
     CDS             14817..>15943
                     /gene="queG"
                     /locus_tag="J8V14_001881"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBA0921982.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQ"
BASE COUNT         5165 a         2994 c         3215 g         4569 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaa
//
LOCUS       DADQRX010000062        15742 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.063, whole genome shotgun sequence.
ACCESSION   DADQRX010000062 DADQRX010000000
VERSION     DADQRX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.063"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="J8V14_001882"
     CDS             complement(24..722)
                     /locus_tag="J8V14_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HBA0921983.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="J8V14_001883"
     CDS             complement(715..2067)
                     /locus_tag="J8V14_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBA0921984.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="J8V14_001884"
     CDS             complement(2290..3690)
                     /locus_tag="J8V14_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBA0921985.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="J8V14_001885"
     CDS             3851..4588
                     /locus_tag="J8V14_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBA0921986.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="J8V14_001886"
     CDS             4713..5192
                     /locus_tag="J8V14_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBA0921987.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="J8V14_001887"
     CDS             5189..5956
                     /locus_tag="J8V14_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921988.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="J8V14_001888"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="J8V14_001888"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA0921989.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="J8V14_001889"
     CDS             complement(6859..7461)
                     /locus_tag="J8V14_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA0921990.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="J8V14_001890"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="J8V14_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="J8V14_001891"
     CDS             8142..8726
                     /locus_tag="J8V14_001891"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBA0921991.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="J8V14_001892"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="J8V14_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBA0921992.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="J8V14_001893"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="J8V14_001893"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA0921993.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="J8V14_001894"
     CDS             10627..10806
                     /locus_tag="J8V14_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921994.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="J8V14_001895"
     CDS             10828..11853
                     /locus_tag="J8V14_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBA0921995.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="J8V14_001896"
     CDS             12028..13272
                     /locus_tag="J8V14_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBA0921996.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="J8V14_001897"
     CDS             13425..14372
                     /locus_tag="J8V14_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0921997.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="J8V14_001898"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="J8V14_001898"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBA0921998.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="J8V14_001899"
     CDS             complement(15274..15516)
                     /locus_tag="J8V14_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0921999.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="J8V14_001900"
     tRNA            15637..15709
                     /locus_tag="J8V14_001900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DADQRX010000063        15314 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.064, whole genome shotgun sequence.
ACCESSION   DADQRX010000063 DADQRX010000000
VERSION     DADQRX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.064"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="J8V14_001901"
     CDS             278..787
                     /locus_tag="J8V14_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0922000.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="J8V14_001902"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="J8V14_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA0922001.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="J8V14_001903"
     CDS             2322..3302
                     /locus_tag="J8V14_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0922002.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="J8V14_001904"
     CDS             3424..4842
                     /locus_tag="J8V14_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBA0922003.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="J8V14_001905"
     CDS             4913..6382
                     /locus_tag="J8V14_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0922004.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="J8V14_001906"
     CDS             6392..6859
                     /locus_tag="J8V14_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBA0922005.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="J8V14_001907"
     CDS             6895..7767
                     /locus_tag="J8V14_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0922006.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="J8V14_001908"
     CDS             7849..10383
                     /locus_tag="J8V14_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922007.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="J8V14_001909"
     CDS             10539..11312
                     /locus_tag="J8V14_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0922008.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="J8V14_001910"
     CDS             11305..12096
                     /locus_tag="J8V14_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA0922009.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="J8V14_001911"
     CDS             12106..12597
                     /locus_tag="J8V14_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922010.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="J8V14_001912"
     CDS             12615..13025
                     /locus_tag="J8V14_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA0922011.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="J8V14_001913"
     CDS             13029..14429
                     /locus_tag="J8V14_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBA0922012.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="J8V14_001914"
     CDS             14452..14826
                     /locus_tag="J8V14_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBA0922013.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DADQRX010000064        15255 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.065, whole genome shotgun sequence.
ACCESSION   DADQRX010000064 DADQRX010000000
VERSION     DADQRX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.065"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="J8V14_001915"
     CDS             248..1021
                     /locus_tag="J8V14_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBA0922014.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="J8V14_001916"
     CDS             1018..1785
                     /locus_tag="J8V14_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBA0922015.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="J8V14_001917"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="J8V14_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBA0922016.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3753..4070
                     /locus_tag="J8V14_001918"
     CDS             3753..4070
                     /locus_tag="J8V14_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBA0922017.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            4070..4420
                     /locus_tag="J8V14_001919"
     CDS             4070..4420
                     /locus_tag="J8V14_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBA0922018.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="J8V14_001920"
     CDS             4587..5525
                     /locus_tag="J8V14_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBA0922019.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="J8V14_001921"
     CDS             5540..6370
                     /locus_tag="J8V14_001921"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBA0922020.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="J8V14_001922"
     CDS             6414..7439
                     /locus_tag="J8V14_001922"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBA0922021.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="J8V14_001923"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="J8V14_001923"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBA0922022.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="J8V14_001924"
     CDS             8517..8957
                     /locus_tag="J8V14_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBA0922023.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="J8V14_001925"
     CDS             8960..9562
                     /locus_tag="J8V14_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBA0922024.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="J8V14_001926"
     CDS             complement(9625..10728)
                     /locus_tag="J8V14_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBA0922025.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="J8V14_001927"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="J8V14_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBA0922026.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="J8V14_001928"
     CDS             12294..12689
                     /locus_tag="J8V14_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922027.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="J8V14_001929"
     CDS             12689..12940
                     /locus_tag="J8V14_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922028.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="J8V14_001930"
     CDS             12983..13378
                     /locus_tag="J8V14_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922029.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="J8V14_001931"
     CDS             13375..14631
                     /locus_tag="J8V14_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBA0922030.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="J8V14_001932"
     CDS             14643..14966
                     /locus_tag="J8V14_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922031.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="J8V14_001933"
     CDS             14959..15225
                     /locus_tag="J8V14_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922032.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DADQRX010000065        14717 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.066, whole genome shotgun sequence.
ACCESSION   DADQRX010000065 DADQRX010000000
VERSION     DADQRX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.066"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(510..1076)
                     /locus_tag="J8V14_001934"
     CDS             complement(510..1076)
                     /locus_tag="J8V14_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA0922033.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="J8V14_001935"
     CDS             complement(1076..2032)
                     /locus_tag="J8V14_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBA0922034.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="J8V14_001936"
     CDS             complement(2032..2688)
                     /locus_tag="J8V14_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBA0922035.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="J8V14_001937"
     CDS             3058..5076
                     /locus_tag="J8V14_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBA0922036.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5503..7917
                     /locus_tag="J8V14_001938"
     CDS             5503..7917
                     /locus_tag="J8V14_001938"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBA0922037.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="J8V14_001939"
     CDS             8265..9818
                     /locus_tag="J8V14_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBA0922038.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="J8V14_001940"
     CDS             complement(10199..11983)
                     /locus_tag="J8V14_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922039.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="J8V14_001941"
     CDS             complement(11998..13719)
                     /locus_tag="J8V14_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922040.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="J8V14_001942"
     CDS             13819..14580
                     /locus_tag="J8V14_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA0922041.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4774 a         2725 c         2913 g         4305 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatg
//
LOCUS       DADQRX010000066        14548 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.067, whole genome shotgun sequence.
ACCESSION   DADQRX010000066 DADQRX010000000
VERSION     DADQRX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.067"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            3..815
                     /locus_tag="J8V14_001943"
     CDS             3..815
                     /locus_tag="J8V14_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBA0922042.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="J8V14_001944"
     CDS             812..1612
                     /locus_tag="J8V14_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBA0922043.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="J8V14_001945"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="J8V14_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBA0922044.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="J8V14_001946"
     CDS             3109..4818
                     /locus_tag="J8V14_001946"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBA0922045.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="J8V14_001947"
     CDS             5030..9388
                     /locus_tag="J8V14_001947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBA0922046.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="J8V14_001948"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="J8V14_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBA0922047.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="J8V14_001949"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="J8V14_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBA0922048.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="J8V14_001950"
     CDS             11226..11519
                     /locus_tag="J8V14_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBA0922049.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="J8V14_001951"
     CDS             11516..11827
                     /locus_tag="J8V14_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBA0922050.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="J8V14_001952"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="J8V14_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBA0922051.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="J8V14_001953"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="J8V14_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBA0922052.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4958 a         2583 c         3238 g         3769 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaat
//
LOCUS       DADQRX010000067        13917 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.068, whole genome shotgun sequence.
ACCESSION   DADQRX010000067 DADQRX010000000
VERSION     DADQRX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.068"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="J8V14_001954"
     CDS             131..505
                     /locus_tag="J8V14_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBA0922053.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="J8V14_001955"
     CDS             complement(619..1248)
                     /locus_tag="J8V14_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBA0922054.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="J8V14_001956"
     tRNA            complement(1382..1454)
                     /locus_tag="J8V14_001956"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="J8V14_001957"
     tRNA            1668..1741
                     /locus_tag="J8V14_001957"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="J8V14_001958"
     CDS             1696..1845
                     /locus_tag="J8V14_001958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922055.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="J8V14_001959"
     CDS             2338..3666
                     /locus_tag="J8V14_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA0922056.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="J8V14_001960"
     CDS             3868..4701
                     /locus_tag="J8V14_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBA0922057.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="J8V14_001961"
     CDS             4717..5454
                     /locus_tag="J8V14_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBA0922058.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="J8V14_001962"
     CDS             complement(5609..6577)
                     /locus_tag="J8V14_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBA0922059.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="J8V14_001963"
     CDS             6809..7642
                     /locus_tag="J8V14_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBA0922060.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="J8V14_001964"
     CDS             7676..8515
                     /locus_tag="J8V14_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0922061.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="J8V14_001965"
     CDS             8541..9101
                     /locus_tag="J8V14_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBA0922062.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="J8V14_001966"
     CDS             9300..11021
                     /locus_tag="J8V14_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBA0922063.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="J8V14_001967"
     CDS             11186..12328
                     /locus_tag="J8V14_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBA0922064.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="J8V14_001968"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="J8V14_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBA0922065.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2832 g         4036 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cgttgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DADQRX010000068        13848 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.069, whole genome shotgun sequence.
ACCESSION   DADQRX010000068 DADQRX010000000
VERSION     DADQRX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.069"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="J8V14_001969"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="J8V14_001969"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA0922066.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="J8V14_001970"
     CDS             complement(2260..2511)
                     /locus_tag="J8V14_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBA0922067.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="J8V14_001971"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="J8V14_001971"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBA0922068.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="J8V14_001972"
     CDS             3461..3679
                     /locus_tag="J8V14_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922069.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="J8V14_001973"
     CDS             complement(3968..4480)
                     /locus_tag="J8V14_001973"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBA0922070.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="J8V14_001974"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="J8V14_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBA0922071.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="J8V14_001975"
     CDS             complement(6933..7367)
                     /locus_tag="J8V14_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBA0922072.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="J8V14_001976"
     CDS             complement(7377..8165)
                     /locus_tag="J8V14_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0922073.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="J8V14_001977"
     CDS             complement(8162..9103)
                     /locus_tag="J8V14_001977"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBA0922074.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="J8V14_001978"
     CDS             complement(9280..9867)
                     /locus_tag="J8V14_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBA0922075.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="J8V14_001979"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="J8V14_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBA0922076.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="J8V14_001980"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="J8V14_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA0922077.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="J8V14_001981"
     CDS             complement(12784..13719)
                     /locus_tag="J8V14_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0922078.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3828 a         3099 c         2442 g         4479 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcatt
//
LOCUS       DADQRX010000069        13513 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.070, whole genome shotgun sequence.
ACCESSION   DADQRX010000069 DADQRX010000000
VERSION     DADQRX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.070"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..1974
                     /locus_tag="J8V14_001982"
     CDS             91..1974
                     /locus_tag="J8V14_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA0922079.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2049..2333)
                     /locus_tag="J8V14_001983"
     CDS             complement(2049..2333)
                     /locus_tag="J8V14_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBA0922080.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2323..3057)
                     /locus_tag="J8V14_001984"
     CDS             complement(2323..3057)
                     /locus_tag="J8V14_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBA0922081.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3186..3809
                     /locus_tag="J8V14_001985"
     CDS             3186..3809
                     /locus_tag="J8V14_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBA0922082.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4001..5122
                     /locus_tag="J8V14_001986"
     CDS             4001..5122
                     /locus_tag="J8V14_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBA0922083.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5129..8257
                     /locus_tag="J8V14_001987"
     CDS             5129..8257
                     /locus_tag="J8V14_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBA0922084.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8276..11470
                     /locus_tag="J8V14_001988"
     CDS             8276..11470
                     /locus_tag="J8V14_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBA0922085.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11616..12323)
                     /locus_tag="J8V14_001989"
     CDS             complement(11616..12323)
                     /locus_tag="J8V14_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBA0922086.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            13088..13486
                     /gene="spxA"
                     /locus_tag="J8V14_001990"
     CDS             13088..13486
                     /gene="spxA"
                     /locus_tag="J8V14_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBA0922087.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         4636 a         2340 c         2804 g         3733 t
ORIGIN      
        1 agtagagaaa ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg
       61 aaaaaagttt atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc
      121 cgctacatca ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat
      181 caccttctgc caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta
      241 tctggttatt tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat
      301 atttggcaat tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt
      361 gtgacagctt cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga
      421 gttgtggtca gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct
      481 tatttcgatc gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa
      541 ggtcaaaact atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa
      601 agaaaatgga tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg
      661 atcttgtata caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg
      721 ttttctatat ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa
      781 aacatcccga aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca
      841 ctgattcttt gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg
      901 ctcctagtga gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt
      961 ttgaatcgat ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt
     1021 tatctgtatc agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat
     1081 catgttttgt tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct
     1141 tatcgatatg tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg
     1201 aaaggatggt tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca
     1261 ttacttgtag caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc
     1321 gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga
     1381 aacaattcag aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg
     1441 tccaaggcac aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt
     1501 atgttggatg ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat
     1561 gtcggacgac aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg
     1621 ttgaaagaca ctgttctgat tggactagga acgaatggct cattctcgga agctcagttt
     1681 gatagtgtga tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg
     1741 acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac
     1801 aatatgacgc tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat
     1861 gatcaagtcc atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc
     1921 ttgctaggag gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta
     1981 caaaaaactt caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt
     2041 tttaacattc atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct
     2101 ttttgttttc gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa
     2161 gaaaatactt ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta
     2221 gcaccagtac cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca
     2281 taaaatgtct gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat
     2341 ctcacgaatc tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac
     2401 tttgaatccg tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt
     2461 tccaggtttt gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac
     2521 ctgaatgatt tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt
     2581 tagtagctta cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc
     2641 gattgccagg tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg
     2701 attacagacg actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc
     2761 gatggtgtgg acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat
     2821 gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa
     2881 aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac
     2941 acgggaaaag tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg
     3001 gataatcttg atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga
     3061 ttccctccaa aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca
     3121 aggtggcttg tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag
     3181 agactatgtt ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg
     3241 gaaatgcccg ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg
     3301 ctccccatcg tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat
     3361 tcagctttat ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc
     3421 gatccaatgc ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg
     3481 taaagatttt gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg
     3541 ctacagaact taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc
     3601 cagctgtata tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag
     3661 tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg
     3721 cggagatcga acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg
     3781 atttcaaata tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg
     3841 aatctactgt cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc
     3901 tgccctcttt ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa
     3961 gtgttataat aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc
     4021 cgattggcat atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt
     4081 actatcttct atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg
     4141 agacttatat gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat
     4201 ggtcgattgg aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag
     4261 cagtacgaga ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac
     4321 acagctagca gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc
     4381 ttactttgaa ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa
     4441 gcaagcggta gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca
     4501 agtattgatt agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat
     4561 cgaagtaggc gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc
     4621 actaggacat ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc
     4681 cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct
     4741 gacaacaggc ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat
     4801 aacagcgagt ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa
     4861 ttacatctat atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg
     4921 gcaaatatat cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa
     4981 gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag
     5041 tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca
     5101 agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt
     5161 tggtcctttt atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt
     5221 gatcagcggg aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact
     5281 ttttggtgaa acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac
     5341 accagacgaa gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat
     5401 cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc
     5461 agccaaagta acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa
     5521 acgtacagaa gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc
     5581 tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga
     5641 taaagagact gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg
     5701 attaaaagaa aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca
     5761 tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc
     5821 cgttttagcc caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa
     5881 agaagctcta acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt
     5941 tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt
     6001 agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac
     6061 gcaacgatta gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact
     6121 gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga
     6181 gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca
     6241 aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca
     6301 agacgcagaa caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta
     6361 tcaaaatctc gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca
     6421 ggaaaaactg caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca
     6481 gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga
     6541 actcaaaaaa gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga
     6601 acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt
     6661 tttgcgtcca ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt
     6721 aaaacacgaa cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc
     6781 tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa
     6841 tcttcaacaa caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc
     6901 tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca
     6961 aaaaacaaaa acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat
     7021 ggccaatgca agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca
     7081 attagctgtc tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg
     7141 agaaatcaaa agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga
     7201 gatacgacga cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa
     7261 agcaggagtg caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca
     7321 tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa
     7381 gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca
     7441 agcacaatat aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac
     7501 agctgaccgg ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga
     7561 agagatcagc caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat
     7621 atacggttta caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat
     7681 ttatcacaca agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac
     7741 attcaacgga gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct
     7801 tcaagaagtg ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca
     7861 atttgagcta gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat
     7921 ctatgatgat caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt
     7981 tattgcagca ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg
     8041 tgtagcaatc gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt
     8101 ggaaatggca atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat
     8161 tagtcatgta agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg
     8221 tagcggacaa agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa
     8281 atggagtatt ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt
     8341 gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga
     8401 attgtatatg gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta
     8461 ttgggaggaa cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa
     8521 aaatctatct cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag
     8581 tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc
     8641 ggtgcaacct ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc
     8701 agagctttct attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac
     8761 atatatcgta aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac
     8821 tgttaataaa cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa
     8881 aatactcacg catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt
     8941 acaagtcaaa ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt
     9001 cctgttagag cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga
     9061 tcaaggaata caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg
     9121 agaagattat ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg
     9181 gggagttatc caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac
     9241 ttggaaacaa ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt
     9301 accgtctttg tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc
     9361 ccctgcagta gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa
     9421 agcaaaagga aaaatccaag ctcgagtcga tttcgtctat ggtgaagtca tctattcgac
     9481 tgacccgaag catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga
     9541 agagaaacga gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta
     9601 tgaacgtcca ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt
     9661 cttccgtcag tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc
     9721 gcataaacat caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt
     9781 cgatgtgaca gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa
     9841 tgatgctttt tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca
     9901 acaaacaagc caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat
     9961 ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac
    10021 ttttagtgaa tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc
    10081 acagcttccc aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg
    10141 gttgaagatg ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg
    10201 gaaaacacta cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc
    10261 tgctttgatc gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt
    10321 cgccccttct ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact
    10381 ggctaaagac acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa
    10441 ttatcagtcg caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag
    10501 cagtacgaaa actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag
    10561 tgggacacca atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc
    10621 aggatttttc ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat
    10681 gattcgaccg tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa
    10741 aacagagatg aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta
    10801 cttgcgtcaa atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg
    10861 gatcgggata cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt
    10921 tattgaagat taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc
    10981 tgccaaagag aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat
    11041 cttgcaagaa gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa
    11101 accacaagat cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct
    11161 gatttcctta aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt
    11221 gtatgacctt tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat
    11281 cggacaaaaa aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg
    11341 catggattca ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga
    11401 agaacagttg acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat
    11461 cgaggagtaa gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag
    11521 cgattgttta tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt
    11581 ccttgctatc atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc
    11641 tctaacagct gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg
    11701 cgttctcgat ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca
    11761 ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca
    11821 gccagctgaa aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata
    11881 ttgcgggaag gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca
    11941 gtcggcccat ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt
    12001 tttttgacct cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat
    12061 aagaggccat cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc
    12121 cgatgtgctc gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca
    12181 tcactatgat ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga
    12241 acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca
    12301 tcaaacacaa ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca
    12361 caaatccttg actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt
    12421 tcttactata tttctatttc cttgacagaa tatggttttt atatttcagt ttttttcact
    12481 aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg
    12541 attgatttat tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag
    12601 tctcaatata actattcaaa cataaaataa cgttatttga tttatttgtt tatagtaaat
    12661 tttatttagt aatcattgct ttaaaatact acgcttatat aacagagaaa ataagcgtta
    12721 taataaagaa cagtattatt taaaatgaca gaaaacatta ctattaaaga aataaaaaac
    12781 ataaaaaatc ttaagaaatc ggtttcattt agcttttgag ataaaataat agtacagttt
    12841 tatacgctgt ttcatatgtt tagaaaattt gaattagatt tgaaaactct aatttttaaa
    12901 tgataaaaaa agcgagaatg ctggttttac tgtatattaa ccttagataa ctttcatttt
    12961 tttaaaaaaa ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca
    13021 gatgggagat tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg
    13081 gtgcacgttg ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc
    13141 gtggttgcaa gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa
    13201 cagttctgaa ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc
    13261 gacacgttcc aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga
    13321 attattggaa cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga
    13381 taaacgtctg caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt
    13441 acgacaatta gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa
    13501 acttggaaaa gac
//
LOCUS       DADQRX010000070        13320 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.071, whole genome shotgun sequence.
ACCESSION   DADQRX010000070 DADQRX010000000
VERSION     DADQRX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.071"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            287..2026
                     /gene="yfcC"
                     /locus_tag="J8V14_001991"
     CDS             287..2026
                     /gene="yfcC"
                     /locus_tag="J8V14_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBA0922088.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2183..3367
                     /gene="iadA"
                     /locus_tag="J8V14_001992"
     CDS             2183..3367
                     /gene="iadA"
                     /locus_tag="J8V14_001992"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA0922089.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3478..3681
                     /locus_tag="J8V14_001993"
     CDS             3478..3681
                     /locus_tag="J8V14_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBA0922090.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3782..4444)
                     /locus_tag="J8V14_001994"
     CDS             complement(3782..4444)
                     /locus_tag="J8V14_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922091.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4457..5959)
                     /locus_tag="J8V14_001995"
     CDS             complement(4457..5959)
                     /locus_tag="J8V14_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922092.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6507..8186
                     /locus_tag="J8V14_001996"
     CDS             6507..8186
                     /locus_tag="J8V14_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBA0922093.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8313..8792
                     /locus_tag="J8V14_001997"
     CDS             8313..8792
                     /locus_tag="J8V14_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBA0922094.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9011..9691
                     /locus_tag="J8V14_001998"
     CDS             9011..9691
                     /locus_tag="J8V14_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA0922095.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9699..11030
                     /locus_tag="J8V14_001999"
     CDS             9699..11030
                     /locus_tag="J8V14_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBA0922096.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11045..12007
                     /locus_tag="J8V14_002000"
     CDS             11045..12007
                     /locus_tag="J8V14_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBA0922097.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12118..12456
                     /locus_tag="J8V14_002001"
     CDS             12118..12456
                     /locus_tag="J8V14_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBA0922098.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12535..13242
                     /locus_tag="J8V14_002002"
     CDS             12535..13242
                     /locus_tag="J8V14_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBA0922099.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4275 a         2334 c         2905 g         3806 t
ORIGIN      
        1 gcaatgaaat tcaatttttc aatgattgta aaatgaaaaa aacaggacga gattttctct
       61 aatctaaaaa taaaaaaaga attttaagga aaaacagaga aataaaatac ttaatataaa
      121 tggaaagaga aaaaaataat catacatagg agaaaaaaaa caaaatagaa aaaagtgatt
      181 ttctaataaa ttattcatgt ggaaatgtca tttttactgt ataattcatt tgattatcca
      241 ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa
      301 ttcttcagga aaaccgaaac gcgacttcac taaattatcg acaccccata cgtatgtcat
      361 tatttttggt gtagtcatct ttgcatggat actgactttt gtcgttccag caggaaagtt
      421 cagcacgcaa gatattgaat acaaagatgc aaatggcgaa acaagtactc gtactgtttt
      481 acgtcaagat tccttccgtt atgcttacga gctggataag tcgtatgtgt ttgatcaatt
      541 agaagaactg caagatcatc cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt
      601 ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc
      661 cttgacagat gatgtgcttt acgatcagta cggcgaaaat atctatgata cttctaaaaa
      721 attgcataaa acagccaaaa tatggggaac agatgatttt ggcggctttg gtttcttaaa
      781 ttttgttttt gaaggactag tatctggaga taagtatgga tctgctgtag ggatcgctgc
      841 attgatcctt gttgttggag gtgcttttgg tattattatg cgtaccggag cgatcgacgc
      901 aggtatctat gcgtttatca gtaaaactaa gggactggag cgtttagctt taccgctatt
      961 attctttgct ttttcattcg gtggcgctac attcgggatg gcagaagagg taattccatt
     1021 ctcaatggtg atggttccat ttgtcattgc gttaggatat gactcgatcg tagcagtaac
     1081 cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt
     1141 agcggtggct caagggattg ctggaatccc cgtgttgtct ggtgcgacat tccgattgat
     1201 tatgtgggta gttgttactg cattagccgc tggttatatg atgatatatg cagaaaaaat
     1261 ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat gattatttcc gttctcatat
     1321 caaaaagaca tctgatgaaa ataaaaaatt catgcttggg cataaattga tcttattaga
     1381 gatgttagtt gtccttgtct ggatcgtttg gggcgtgact caaaaaggct attacattcc
     1441 agaaatcgct tctcaattct ttgtgatggg attagttgct ggaatcattg ctgttctttt
     1501 caaattagat ggaatgaaag tcaatgacat cgcttcttct ttccaatctg gtgcagctga
     1561 tttagctggt acagcaatcg tagtaggtat ggccaaaggg attcttcttg tcttgggagg
     1621 atctgatgca tctgttcctt cagctttgaa tacgattttg catgggatcg ggacggcgtt
     1681 gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa
     1741 cttggttgtt acatcaaatt caggacaagc agctttgact atgccaatca tggctccact
     1801 ggcagattta gtaggtgtat ccagacaagt agcagttctt gcttatcagc ttggttcagg
     1861 ttttatggac gcttttacac ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg
     1921 gatcgaatgg gcaaaatggg caagattcca aatcaagatg caaggattcc tgtttgtttt
     1981 aggaactatt ttcatcatga ttgccattgc aataggtctg cagtagtact ggagacctgt
     2041 aaataaaaaa gtaatccaaa cagtagataa ggactccaag aaccgtcatt ttacggtaaa
     2101 atcaaaggag taaagatcct atcgtttgga ttatttttta agatcctatc atcagttata
     2161 cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga
     2221 ttacttagga aaaatggatg tactgattgc cggtgggaag attttggctg tagaagaaca
     2281 gctagaaggc ggctacgaag gagtggaagt agaagaactt tcaggtgagg gaaaagtact
     2341 aaccccgggt ttcattgact gtcatttcca tattttaggt ggaggaggag aaggcggtta
     2401 tcaaaatcga acgccagaag tcacattgag ccaattgact acagctggtg taacgacagt
     2461 tgtcggttgt ttgggaacag acggagaagg tagagatatg actgcgttga tcagcaaagc
     2521 gaaaggtctg gaagcagaag gaatctccac ttatgtatac gaaggttcgt accgattgcc
     2581 ggtaaaaaca gtaacagatt ctatcatcaa agactttttg acaattgata aaattatcgg
     2641 aatcggagaa atcgcagtat ccgatcaccg ttcttctcag ccaagttttg aagagttcgc
     2701 tcgtgcagta gcagatgctc gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa
     2761 tgtccactta ggcggaggaa aaagaaagct agagctattg acacgaatcg tagaagaaac
     2821 agaaattccg atcacacaat ttttgcctac tcatgcaaat cgcacaccag aattatttga
     2881 agcttgcgtg gcatttgcca aacgtggtgg cacgattgac ttcactgcta gcgaagatcc
     2941 tgatttttgg gagaagacag atggtgaagt tcgcttcagc aaagcactga aacgattgat
     3001 cgaggaagat gtttctttag ataatttcac gatgacctca gatggacaag gaagcttgcc
     3061 ttattttgat gaaaacaatc atttcctagg gttaggagta ggaagtgcca aggcattgct
     3121 agtagggatc aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc
     3181 tataaccagt aatccagcac gtatattgaa attggataaa aaaggaaaaa tcgaaattgg
     3241 tgctgatgca gatctgtgta tcttggataa agaaacgctt gatattgata cagttatcgc
     3301 taaaggagaa ataatggtcc aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag
     3361 tttttaatca acagctctaa ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc
     3421 tgttgttgat gttaccctga aggtagatca ataaacaaac taagaggagg aagcacaatg
     3481 gcagatttaa aaggacgttc taaagatatg aaagataaag tagtcggtga aggaaaagaa
     3541 gcatacggca aagcaacaga cgacaagagc aaagaaacag aagggaaagc acaatcacta
     3601 ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga caagttttct
     3661 cgaaaagacg aaaaaaaata aaaaccactt attactaatt gaatcaagca aataaaaaat
     3721 ccgaaccata tctgcgagac agatatggtt cggatttttt atttgatcat tatcctgttt
     3781 tttaaacttc gattttttga aattttttat tttttagttc aaagacttca tcacagcttt
     3841 ttgctacttc aatctcatga gtcacgatga taatacagcg attttgttca tgggcgattt
     3901 cttggaaaag tttgacgata tcttgtgaag ttgtttcatc tagatttcca gtaggctcat
     3961 ccgctacgat tagttgatgt tcacagcaga tagcacgaac gatagcgact cgctgctgct
     4021 gacctcccga aagtttggtc acctgttttt ttgctaatat ttcatcaatt cctacacgag
     4081 ccagtcgatc taaagcaact tgtcgataat tctcttgctt ggtcttagtg atttccattg
     4141 ctgctatgac gttgtctaga gccgataaat aaggaagcaa gttgtaagct tggaaaataa
     4201 tcgagacatc atttttacga taacttctta acccgatttt gttcagtgaa cgatcttgat
     4261 agtatacctt tccttctttt ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt
     4321 ttccagaacc acttgctcct aaaatagcgt acatttttcc agcttcgaaa ttcagttcta
     4381 catctttata aagcgcatct tgcggatctt gataccaaaa tcccaattgt tctgtccgta
     4441 acatcaaatt cctcctttag tttaatagga tttttcttgg gtttaatctt aatatcccta
     4501 tagaagctaa aaagacagaa aacagactga ttccaaatgc tactccggct aatagtccca
     4561 attgtgcagg tttgacattg atttccaatt cgctgacttg atcagaaaca gcaaatggat
     4621 tattcgtggt tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca
     4681 tttgtccacc gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta
     4741 gctgctgtcc cacggcattc gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa
     4801 gaatcataca aatagccact tcggtaaaca attggagaat gattttactt ctggattctc
     4861 ctaaagatag caagacgcct aactcatgcc gacgctcccg aatggtaatc ataatgatca
     4921 gagtaagtat gataataccg gctacagcca ctagtaaaac aacattttta gaaaaacttg
     4981 ccacgttgtt taaaggttcc aacattgact gatacatgga atcatttgat tgtaagctaa
     5041 atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt ttatctggat
     5101 cagatagagt gtaaatagca gaatctatcg tgttatcgtc agatgtccca tttagttcat
     5161 ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt catgaagttg aatttcatac
     5221 ccatactgcc gcttgtttct ctggattcat aaattccttt gatcgtcatt tcataagtgg
     5281 tatcttcatc ttcagtactc gtgattgtga aagtatctcc gactgccagg tcatttgttt
     5341 ctgctagagt agaatcaatg actacattgt ttgtgccttt gtcgctgtca tctattcctt
     5401 caccatccgt gatttttgct gttccatcag aaaaattact attttgtgca gttgctgaca
     5461 caccactgat ttgaaagtct gcttgagtca tttgcggatt cccgcccatt cctccagcag
     5521 gttcaccgcc tgcatcagta gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg
     5581 cagttattcc atcttttgcc atagctgagg cggaactttc aaaggaataa ctctttacat
     5641 tatctaattc agctattttt tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc
     5701 ctggatctgg acgttcttca gtggtagaac cgcttgaaga agtttgttcc tgttttttaa
     5761 atgaagcttc tcgattggca cttaaagtca ctgttgctcc aacttctttt tgtgcttgat
     5821 tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg
     5881 ctgtgaacac ggcaaacaat aacaatgaac gtccccattt tacttttgta cttttccatg
     5941 cacgtttcat aaaattcata ttttgcatcc ctcctagtag atgcatttga taatagacaa
     6001 cctatcttaa agctagctta aatcttatgc tgtttgcaca ccatcaacat gaggttctgt
     6061 atatataaaa agtcactata gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt
     6121 caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga aatcctagat gctacagagg
     6181 gatttttacg tgcatagttg ttctaacaat tgctgatact agcgatttag tttatttgtt
     6241 gtataataat ggggaacagc tgaatactta tataagtatt ctggctatag cgtttcgcaa
     6301 aagatccatt ttgttaatga agtatatcca aaataaaatg cccatccaac tgtcagaacg
     6361 cggatgtgct ggtaattttc ctgctgattc tccataagtt gagaaatagt gattttgcat
     6421 atgcttcatt ggaatggctc atatatttag ataaagaaga aaatcgacat ctttaaactt
     6481 ggatgtcata agaagaggtg aagtttgtga gtaccattaa aattatccca ttaggcggcg
     6541 ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt tttattttag
     6601 attgtgggct aaaatatcct gaaaatgagt tgttggggat cgacgtcgta atccctgatt
     6661 ttacgtattt agaagaaaac attgatcgtg tagctggtgt ctttttaaca catggacatg
     6721 cagatgcgat cggggcactt ccttacttgt tgtcaaaaat ttctgtacca gtattcggga
     6781 cagagttgac gatcgagtta gcaaaattga atgtcagtcg taatgaagca gccaaaggat
     6841 ttaaggattt ccatgtggta gatgagcata cagaaataga ctttggtgaa acagtagtga
     6901 gctttttccg taccactcat acgataccag attcagtggg gatcaatctg aaaacaaaag
     6961 aaggtagtat cgtttatacg ggagatttta aatttgacca aacggctatc ccaatgtatc
     7021 aaacagatta tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg
     7081 aatcggcaaa tgcagaaaat cctcagcctg tagcttctga agcccaaata gcagatgaag
     7141 tatttgatac gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt
     7201 tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga
     7261 ctggtcaaga tttcgaacga attattcgca cagcgatgcg tttagaaaaa cttcaattac
     7321 caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca gaagaactga
     7381 tcattttaga aactggccga atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg
     7441 gtacacaccg taatgtcaag attgaagaag gagatttggt ttatatcact actacgccaa
     7501 gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat catttatcga gctggtggaa
     7561 cagtgaagtt gatctctgag aatatgcgcg tttctggcca tgccaatcct aatgacttgc
     7621 aattaatgat caatttgatg aaaccaacat attttgtgcc agttcaagga gagtatcgtg
     7681 aattagctgc tcatgcagat ttggctcatg cagtagggat gccatacaaa aatatctata
     7741 ttactggacg cggagacgtg ctggaatata agaacaagcg tatgagtgtt gcaggaacta
     7801 caagtgcaga aaatgtaatg attgatggat taggcatagg tgatatcgga aacattgttt
     7861 tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt agcggttgtc actatcagtc
     7921 ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc acggggattc gtttacgtaa
     7981 aagctagccg tgatctgatg aaagaaagca gcaatatgat tgaaacaatc gttgagaaac
     8041 atttagaaag caatgatttt gagtggagca aattaaagca agacatccga gatcaattag
     8101 gccgttactt atttgaacaa acaaaacgcc gcccagtgat cttgccagtc attatggaag
     8161 ctactcaacg tcgaaataaa aaataaacaa aaagagtaag atgaacctct ctatagagtg
     8221 ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg aatacagcat ccctcgcttt
     8281 ataataaacg atagaataaa gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt
     8341 atttagaaga acaaaagatc agctatcaac cacttgattt tagtcatgtg gtagaagaat
     8401 ctttcacaga agaactacaa aaaagaggga ttgatccagc tttgatatgt aaaactctcg
     8461 tagtgaaagg aaacaaaact ggggtgatca tcgcattgat tcctttgaac gatcacttag
     8521 attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt tggttttcct ggaatggatt
     8581 ttgtattaat gcatacagga tatccccacg gagcaaacac ccctattgga ataaaactcg
     8641 cacatcctga ttattgtttt ttagcagaca gttctttaaa agaaaaagaa gaagtaattg
     8701 tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt agccgattta gaaaagatcg
     8761 tccatcctat ttacgctgat ttaattagtt aagggacgag aagagaagct aagcagagca
     8821 gagacctcaa acttgagatt atatctgctt ggtacttggt gcatcctgta ttatctgtta
     8881 caattgcttt gtacatactg tactatcaat ccaatgaaaa tatatttagc agctatattt
     8941 tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga
     9001 ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt ttaacaattg aaatggaaaa
     9061 tggccaacgc gtgatgattg acccgtttat tacaggaaat ccattgacag acttaactgc
     9121 agaagaagta aagacagatt ggttgctgat tactcatggt catagtgacc acataggaga
     9181 tatgattcct attgcaaaag aaaatcaagc cacaattatc agtattgttg agattgctga
     9241 ttatgcgaac agcagaggag tagattcatt cggtatgaat atcggtggaa aacatgcgtt
     9301 tccattcggc gtggtaaaat tcgtacatgc acagcatagt tcaagctatg aagtagacgg
     9361 tatcgttcag tatatgggcg agcctagcgg aattattatt caagcagaag gaaaaacgat
     9421 ttatcatgca ggggatactg cttatttcag tgatctagga ttgcttgcag aagaattcga
     9481 tattgatgtc gctttcttgc cgattggcga taactatacg atggggccag aagatgcagc
     9541 aaaggctgcg caaacaattc gtgccaagaa agtagttcca atccattaca atactttccc
     9601 tgtcattgaa caagatcccg aatcttttgt agagttactg gaagatagtg aaggaaaaat
     9661 aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa catgatttga
     9721 ttttagaata tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa
     9781 aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg
     9841 gactagtttc aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac
     9901 atattgaacg tttgacattt ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg
     9961 gggcggcagg attagacaag gtcttgaata aattcgtcat cggcgcgatg actaaacatg
    10021 ccatggaacg ctatattacg ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc
    10081 aaaaattagc attggaagat ggcgccgcag tattgatcac aggaggattc aatacgactg
    10141 ctgagattgc aaagctagct gatgatcttg aaatgccgat cttacgcacc tcttatgata
    10201 catttacagt cgcaaccatg atcaaccgag cgttaagcga ccaattgatc aaaaaagata
    10261 tcatgcttgt cagtgatatc tatatgccag ctgaaaagac tcattatttg cagcagacag
    10321 atacgatttc tgattatcag cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg
    10381 tcaaccgcca tcatcgtttg atggggattg ttacggcaaa agatgtttta gggaaatcgc
    10441 caaaccagat catcgatcgg gtgatgacga aagaaccaat cagtgtaaag aaaacaatga
    10501 gtattgcctc tgttagccat caaatgatct gggatgggct agaagtcatg ccggtcgttt
    10561 ccgatgactt gacattggaa ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc
    10621 ttgtccagcg acaaccgcag attgcagata cgatttcgga tcagatatct ggaaatatcc
    10681 atacgattga tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag
    10741 tagcaccaca aatggtcaat ggcgttggaa ccatttcttt tggcgtactt agcgaaatca
    10801 tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc ttgattgaac
    10861 aagtgaacct acattatttg cggctgatcc aattggaaag cgaattagac attcgtccac
    10921 gtgtattgga aatcggcaga cgttccgcta aactggatat cgaagtgttt attgaaaaca
    10981 ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga acgttcttag aaagcaggag
    11041 tgaaatgaca gtacagcaag aaatagtaga tacaataaaa aaatacgata aaatcattat
    11101 ccacaggcat ttgcgccctg atcctgatgc attaggatca caagtaggat tagccgaact
    11161 attacgggaa agtttcccag aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct
    11221 tcgcttttta gcagaaatgc aagaagttga agatgtcttc taccaaggag cgctagtcat
    11281 cgtgactgat acagctaatt cacccagaat cagtgatcaa agatatcagt tggcagaaaa
    11341 actgatcaaa atcgaccatc atcccaatga tgacccttat ggagatcttg tgtgggtaaa
    11401 tacaaaagca agtagctgta gtgagatgat tgcagaattt gctttgatgt ttcctgaatc
    11461 tttaaagatg aacgttaagg ctgcacggtt actttatgca ggaatcgtcg gagatacagg
    11521 gcgctttttg tatccctcca cgacgtcccg tacattagag attgcttcga tattgagaaa
    11581 ttatccattt gatgcaagtg ctttgaacag agagattgaa caaatgccga tgaaagtcgc
    11641 taaactttcc ggttatcttt atcaaaatat ccaggtggat gaaaatggtg caggaagaat
    11701 aatccttccg caagaaattc ttgataaata tggaatcgat gattcagaga cagcttcgat
    11761 cgtttcactt cctggagtga ttgacgaagt gctggcttgg gggatttttg ttcagcagcc
    11821 ttcagggtat taccgcgtac gtttacgttc taaggggccg atcatcaatg aattggctaa
    11881 acgccatcat ggaggaggcc atccattagc aagtggtgcg aatgcaaaag atttagcaga
    11941 agtagaggaa atctataaag agatccaaga tatatgccaa tcgtataaga gcagaaaaac
    12001 agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc
    12061 aagatcttct ttttccgtta caatagtgct cactaaagat tttaaaggag aaagaagatg
    12121 acattaccaa attgcccaga atgtggctca gagtacacat atgaagatcg aggcttgttg
    12181 atttgcccag aatgcgggaa cgaatggaac aaagaagaac aagaaactgt catagaagac
    12241 ggattagtgg tattagatgc aaatggaaac cgtctgttag agggcgatag cgtgacagtg
    12301 attaaagact tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag
    12361 aacatccgtt tagtagaagg cgatcataat atcgattgca aggtagaagg atttggaccg
    12421 atgaaactga aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt cttttcacat
    12481 tcttttccta ctactaatga taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat
    12541 ggttatggaa tgtatggttt tttagatccg acatttcttt tggtcattct tggattagtt
    12601 atttccggaa ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga tcaagtgaga
    12661 agcaaaaaca atgtgactgg tacacaagca gcacaatata tattacaaag tcaacagata
    12721 aataatgtcg gtgttcaaca aattgccgga gatctcacag ataactacaa ttcaggtaat
    12781 aagatgttga gtttgtctca agcaactgca cagtctactt cagttgctgc gataggtgta
    12841 gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc attgaagatt
    12901 cgtgcagcac ttgttccagc agctaacttt ggttcgatga tttcgttccc cttgattatg
    12961 gtaggtgtat tgcttagctg gaactctacg ttaatcaata tcgggatttt cgctttttct
    13021 ttagcattac ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca
    13081 atccaaattt tgagtgaagg cggactactg acggaagaag aagtaccgat ggcaagacac
    13141 gtattatttg cagcagcact gacttatgtt gcagcagcgc tttcgacatt cctgcagctg
    13201 ttgcgcttga tcttattgtt cggcggcaac cgaagagatt aaaataaaga agttgtgaaa
    13261 tcagcgcttt gatctgagct aataccgaga gttagctgat tgaataccat ttatttggaa
//
LOCUS       DADQRX010000071        13261 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.072, whole genome shotgun sequence.
ACCESSION   DADQRX010000071 DADQRX010000000
VERSION     DADQRX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.072"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            201..1070
                     /locus_tag="J8V14_002003"
     CDS             201..1070
                     /locus_tag="J8V14_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBA0922100.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1279..2304
                     /locus_tag="J8V14_002004"
     CDS             1279..2304
                     /locus_tag="J8V14_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBA0922101.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2343..3293
                     /gene="manA"
                     /locus_tag="J8V14_002005"
     CDS             2343..3293
                     /gene="manA"
                     /locus_tag="J8V14_002005"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBA0922102.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3387..5090
                     /locus_tag="J8V14_002006"
     CDS             3387..5090
                     /locus_tag="J8V14_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0922103.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5092..7125
                     /locus_tag="J8V14_002007"
     CDS             5092..7125
                     /locus_tag="J8V14_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBA0922104.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7136..7576
                     /locus_tag="J8V14_002008"
     CDS             7136..7576
                     /locus_tag="J8V14_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA0922105.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7776..8903)
                     /locus_tag="J8V14_002009"
     CDS             complement(7776..8903)
                     /locus_tag="J8V14_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922106.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8906..9583)
                     /locus_tag="J8V14_002010"
     CDS             complement(8906..9583)
                     /locus_tag="J8V14_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922107.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9847..11613
                     /locus_tag="J8V14_002011"
     CDS             9847..11613
                     /locus_tag="J8V14_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBA0922108.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11628..12065
                     /locus_tag="J8V14_002012"
     CDS             11628..12065
                     /locus_tag="J8V14_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA0922109.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12078..13229
                     /locus_tag="J8V14_002013"
     CDS             12078..13229
                     /locus_tag="J8V14_002013"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA0922110.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4499 a         2304 c         2739 g         3719 t
ORIGIN      
        1 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
       61 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      121 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      181 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      241 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      301 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      361 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      421 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      481 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      541 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      601 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      661 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      721 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      781 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      841 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      901 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
      961 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1021 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1081 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1141 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1201 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1261 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1321 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1381 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1441 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1501 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1561 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1621 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1681 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1741 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1801 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1861 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1921 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     1981 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2041 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2101 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2161 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2221 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2281 gagaaaacgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2341 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2401 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2461 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2521 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2581 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2641 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2701 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2761 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2821 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2881 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     2941 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3001 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3061 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3121 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3181 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3241 agtgggcggt ccaaggtgaa ctagcaatca ttgcgactga accaggcaaa tagttcccag
     3301 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3361 caataaatag aagtatggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3421 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3481 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3541 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3601 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3661 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3721 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccac gtgaaattca
     3781 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3841 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3901 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     3961 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4021 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4081 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4141 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4201 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4261 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4321 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4381 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4441 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4501 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4561 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4621 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4681 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4741 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4801 aacaaaaaaa gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4861 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4921 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     4981 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5041 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5101 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5161 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5221 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5281 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5341 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5401 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5461 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5521 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5581 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5641 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5701 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5761 ctgacattga cgatggagcg tgtgactaaa aatcatttac tgacaaatga gacatataat
     5821 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5881 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     5941 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6001 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6061 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6121 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6181 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6241 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6301 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6361 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6421 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6481 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6541 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6601 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6661 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6721 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6781 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6841 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6901 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     6961 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7021 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7081 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7141 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7201 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7261 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7321 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7381 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7441 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7501 cgaccaagta atggcgctca gtgatgcgaa aaccattgaa gaagtccaaa tgatttttga
     7561 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7621 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7681 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7741 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7801 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7861 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7921 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     7981 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8041 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8101 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gaaggattcg tatctcctga
     8161 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8221 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8281 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8341 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8401 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agggagatgc
     8461 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8521 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8581 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8641 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8701 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8761 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8821 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8881 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     8941 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9001 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9061 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9121 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9181 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9241 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9301 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9361 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9421 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9481 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9541 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9601 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9661 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9721 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9781 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9841 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9901 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
     9961 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt ggctaccatg
    10021 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10081 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10141 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10201 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10261 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10321 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10381 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10441 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10501 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10561 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10621 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10681 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10741 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10801 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10861 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10921 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    10981 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11041 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11101 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11161 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11221 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11281 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11341 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11401 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11461 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11521 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11581 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11641 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11701 gcggtcagat gcttgtagaa gcagggggcg ttgatgagga atatatcgat gccatggtag
    11761 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11821 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11881 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    11941 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    12001 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12061 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12121 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12181 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12241 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12301 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12361 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12421 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12481 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12541 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12601 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12661 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12721 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12781 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12841 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12901 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    12961 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    13021 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13081 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13141 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13201 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13261 a
//
LOCUS       DADQRX010000072        13197 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.073, whole genome shotgun sequence.
ACCESSION   DADQRX010000072 DADQRX010000000
VERSION     DADQRX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.073"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="J8V14_002014"
     CDS             complement(105..629)
                     /locus_tag="J8V14_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0922111.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="J8V14_002015"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="J8V14_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBA0922112.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="J8V14_002016"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="J8V14_002016"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA0922113.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="J8V14_002017"
     CDS             complement(2351..3031)
                     /locus_tag="J8V14_002017"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBA0922114.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="J8V14_002018"
     CDS             3157..4026
                     /locus_tag="J8V14_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0922115.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="J8V14_002019"
     CDS             4052..4567
                     /locus_tag="J8V14_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922116.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="J8V14_002020"
     CDS             complement(4614..5177)
                     /locus_tag="J8V14_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0922117.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="J8V14_002021"
     CDS             complement(5232..5411)
                     /locus_tag="J8V14_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922118.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="J8V14_002022"
     CDS             complement(5488..5997)
                     /locus_tag="J8V14_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922119.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="J8V14_002023"
     CDS             complement(6093..6473)
                     /locus_tag="J8V14_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA0922120.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="J8V14_002024"
     CDS             6803..7552
                     /locus_tag="J8V14_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBA0922121.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="J8V14_002025"
     CDS             complement(7873..9090)
                     /locus_tag="J8V14_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA0922122.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="J8V14_002026"
     CDS             complement(9348..10625)
                     /locus_tag="J8V14_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922123.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="J8V14_002027"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="J8V14_002027"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBA0922124.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="J8V14_002028"
     CDS             complement(11547..12491)
                     /locus_tag="J8V14_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBA0922125.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="J8V14_002029"
     CDS             complement(12493..13179)
                     /locus_tag="J8V14_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBA0922126.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3739 a         2764 c         2390 g         4304 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaa
//
LOCUS       DADQRX010000073        12747 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.074, whole genome shotgun sequence.
ACCESSION   DADQRX010000073 DADQRX010000000
VERSION     DADQRX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.074"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="J8V14_002030"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="J8V14_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBA0922127.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="J8V14_002031"
     CDS             complement(323..892)
                     /locus_tag="J8V14_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBA0922128.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="J8V14_002032"
     CDS             1078..1980
                     /locus_tag="J8V14_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0922129.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="J8V14_002033"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="J8V14_002033"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBA0922130.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="J8V14_002034"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="J8V14_002034"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBA0922131.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="J8V14_002035"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="J8V14_002035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBA0922132.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="J8V14_002036"
     CDS             8554..8739
                     /locus_tag="J8V14_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBA0922133.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="J8V14_002037"
     CDS             complement(8801..9997)
                     /locus_tag="J8V14_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBA0922134.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="J8V14_002038"
     CDS             complement(10167..11603)
                     /locus_tag="J8V14_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA0922135.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="J8V14_002039"
     CDS             11896..12705
                     /locus_tag="J8V14_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0922136.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DADQRX010000074        12742 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.075, whole genome shotgun sequence.
ACCESSION   DADQRX010000074 DADQRX010000000
VERSION     DADQRX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.075"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..544
                     /locus_tag="J8V14_002040"
     CDS             101..544
                     /locus_tag="J8V14_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0922137.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="J8V14_002041"
     CDS             complement(550..1734)
                     /locus_tag="J8V14_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBA0922138.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="J8V14_002042"
     CDS             2002..2700
                     /locus_tag="J8V14_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBA0922139.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="J8V14_002043"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="J8V14_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBA0922140.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="J8V14_002044"
     CDS             complement(3510..4043)
                     /locus_tag="J8V14_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922141.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="J8V14_002045"
     CDS             complement(4340..6721)
                     /locus_tag="J8V14_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBA0922142.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="J8V14_002046"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="J8V14_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBA0922143.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="J8V14_002047"
     CDS             7422..7961
                     /locus_tag="J8V14_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBA0922144.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="J8V14_002048"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="J8V14_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBA0922145.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="J8V14_002049"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="J8V14_002049"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="J8V14_002050"
     CDS             9068..10234
                     /locus_tag="J8V14_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBA0922146.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="J8V14_002051"
     CDS             10280..11776
                     /locus_tag="J8V14_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBA0922147.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="J8V14_002052"
     CDS             11857..12540
                     /locus_tag="J8V14_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBA0922148.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3609 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta tt
//
LOCUS       DADQRX010000075        12670 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.076, whole genome shotgun sequence.
ACCESSION   DADQRX010000075 DADQRX010000000
VERSION     DADQRX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.076"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="J8V14_002053"
     CDS             6..305
                     /locus_tag="J8V14_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922149.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="J8V14_002054"
     CDS             378..1739
                     /locus_tag="J8V14_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA0922150.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="J8V14_002055"
     CDS             1761..2021
                     /locus_tag="J8V14_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922151.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="J8V14_002056"
     CDS             2035..2787
                     /locus_tag="J8V14_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBA0922152.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="J8V14_002057"
     CDS             complement(2881..3486)
                     /locus_tag="J8V14_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0922153.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="J8V14_002058"
     CDS             complement(3539..4513)
                     /locus_tag="J8V14_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBA0922154.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="J8V14_002059"
     CDS             complement(4891..5670)
                     /locus_tag="J8V14_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922155.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="J8V14_002060"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="J8V14_002060"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBA0922156.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="J8V14_002061"
     CDS             7161..8558
                     /locus_tag="J8V14_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0922157.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="J8V14_002062"
     CDS             8639..9079
                     /locus_tag="J8V14_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBA0922158.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="J8V14_002063"
     CDS             9227..9553
                     /locus_tag="J8V14_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA0922159.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="J8V14_002064"
     CDS             9554..10303
                     /locus_tag="J8V14_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922160.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="J8V14_002065"
     CDS             complement(10383..10838)
                     /locus_tag="J8V14_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBA0922161.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="J8V14_002066"
     CDS             complement(11265..11540)
                     /locus_tag="J8V14_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922162.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="J8V14_002067"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="J8V14_002067"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBA0922163.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2588 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DADQRX010000076        12635 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.077, whole genome shotgun sequence.
ACCESSION   DADQRX010000076 DADQRX010000000
VERSION     DADQRX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.077"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..1111)
                     /locus_tag="J8V14_002068"
     CDS             complement(146..1111)
                     /locus_tag="J8V14_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBA0922164.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="J8V14_002069"
     CDS             1345..2190
                     /locus_tag="J8V14_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0922165.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="J8V14_002070"
     CDS             2381..3568
                     /locus_tag="J8V14_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBA0922166.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="J8V14_002071"
     CDS             3574..3930
                     /locus_tag="J8V14_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBA0922167.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="J8V14_002072"
     CDS             3932..4270
                     /locus_tag="J8V14_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBA0922168.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4665..5699
                     /locus_tag="J8V14_002073"
     CDS             4665..5699
                     /locus_tag="J8V14_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0922169.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="J8V14_002074"
     CDS             5692..6378
                     /locus_tag="J8V14_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922170.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="J8V14_002075"
     CDS             6402..7250
                     /locus_tag="J8V14_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0922171.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="J8V14_002076"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="J8V14_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBA0922172.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="J8V14_002077"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="J8V14_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBA0922173.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="J8V14_002078"
     CDS             9516..10751
                     /locus_tag="J8V14_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBA0922174.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="J8V14_002079"
     CDS             10738..11208
                     /locus_tag="J8V14_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBA0922175.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="J8V14_002080"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="J8V14_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBA0922176.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DADQRX010000077        12229 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.078, whole genome shotgun sequence.
ACCESSION   DADQRX010000077 DADQRX010000000
VERSION     DADQRX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.078"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..204)
                     /locus_tag="J8V14_002081"
     CDS             complement(<1..204)
                     /locus_tag="J8V14_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA0922177.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTL"
     gene            complement(201..854)
                     /locus_tag="J8V14_002082"
     CDS             complement(201..854)
                     /locus_tag="J8V14_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0922178.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1142..1662
                     /locus_tag="J8V14_002083"
                     /pseudo
     CDS             1142..1662
                     /locus_tag="J8V14_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1564..2280)
                     /locus_tag="J8V14_002084"
     CDS             complement(1564..2280)
                     /locus_tag="J8V14_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBA0922179.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2404..2589)
                     /locus_tag="J8V14_002085"
     CDS             complement(2404..2589)
                     /locus_tag="J8V14_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922180.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2816..4089
                     /locus_tag="J8V14_002086"
                     /pseudo
     CDS             2816..4089
                     /locus_tag="J8V14_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4196..5227)
                     /locus_tag="J8V14_002087"
     CDS             complement(4196..5227)
                     /locus_tag="J8V14_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922181.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5234..6064)
                     /locus_tag="J8V14_002088"
     CDS             complement(5234..6064)
                     /locus_tag="J8V14_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922182.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6061..6867)
                     /locus_tag="J8V14_002089"
     CDS             complement(6061..6867)
                     /locus_tag="J8V14_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0922183.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6873..7697)
                     /locus_tag="J8V14_002090"
     CDS             complement(6873..7697)
                     /locus_tag="J8V14_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HBA0922184.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7687..8787)
                     /locus_tag="J8V14_002091"
     CDS             complement(7687..8787)
                     /locus_tag="J8V14_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBA0922185.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8965..9750
                     /locus_tag="J8V14_002092"
     CDS             8965..9750
                     /locus_tag="J8V14_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBA0922186.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9783..10166
                     /locus_tag="J8V14_002093"
     CDS             9783..10166
                     /locus_tag="J8V14_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA0922187.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10242..10373
                     /locus_tag="J8V14_002094"
     CDS             10242..10373
                     /locus_tag="J8V14_002094"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0922188.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10342..10578
                     /locus_tag="J8V14_002095"
     CDS             10342..10578
                     /locus_tag="J8V14_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0922189.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10656..11627)
                     /locus_tag="J8V14_002096"
     CDS             complement(10656..11627)
                     /locus_tag="J8V14_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBA0922190.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3672 a         2763 c         2231 g         3563 t
ORIGIN      
        1 caaagtttgt tctatatatt ttatttcttt tccattcata ttactagaaa tttcatttgc
       61 catattatga atcttttctt ttctcgtctc caaatatgtt ttaggaaaga taaaaaaaat
      121 taatgaatga actaatataa ttattattcc aagaacagaa aatatttgta tgaacatttt
      181 tggaaatatc tttaattttt tcattttctc tccaatttat atcctacatt tcttatagta
      241 gttatacagt ctaattgtaa tttttttcta agttctttta tatatacatc tattactcga
      301 tcataaggag tttcttcgct atctttccat acataatcaa taatttgcat tcttgttaac
      361 acttgtccat tattatccaa taaacatttt aatacttcta gttcttttgc atttacatct
      421 atttcttcac catttatttt agctgtatag ctattaaaat taaccgatag atccttatat
      481 tcaaacttct ctaaatgtcc ataattcttc ttaattaaag aatctattct agcttttaag
      541 accggcaatg aaaatggctt ttctacatat ccgtctacta aattagtaaa tgcatcaatt
      601 ttatattctt catcactaaa tgcagtcaag attagaattg gtaaattgct tttctttcta
      661 atttctttca acacttctaa accatttata aaaggtatct gaatgtctaa gataaccaaa
      721 tttatatcgc tattaaattt tgacaaggct tctcttccat ctttagcttg aataacagta
      781 tatccaaatt ctgaaagata ttcacttatt ccctctctta tcatattatc atcttcaaca
      841 gttaatattt tcatatatac ctccacttaa gcctatacac tagatattat acagcatatt
      901 atggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttt
      961 taataaatgc aaacagacac cttgaaatcc tttagaaaaa aggcttctat aatattttgt
     1021 gttgttggaa aatcgaaggc gattttctac taacactttt tcttcgtttc aactcaaaaa
     1081 gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa ggagtcgctc
     1141 tatggataat catactagaa aattactcgg attaacagat aaacatttat tttttgatga
     1201 agaatggctg attgaaaaag aatataaagg cgttcaagcg cagtttatta aagggagatt
     1261 ggatgactca ccttcccatt gtgaacactg tggatcgatt cgtattatcc gcaacggttc
     1321 ctatacgaca cgtactcaga tgttgaaagt caaagaaaag ttgactattt tagaattaaa
     1381 acgtacccgt tttctatgct atgattgtgg gcagaccttt tctgcgaaaa cggacttagt
     1441 tgatgaacat catcaactaa caaaggaact gaagcaggcg attctcatag aactgtacga
     1501 aaatcaatca cgtaatctga aaatcagaag aagtaccgtc ggctcaagcg atattggaaa
     1561 ctcttactca aagattccac gacattggag ccgctcaaac ggcattatca tcgactgttt
     1621 aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat ggtgtcctga
     1681 acgtacagcg acaccttcca tatccagagc agtcgccaca tcacggggat ggatgccatc
     1741 caaattaaaa gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt aaatggtgat
     1801 accgccgatc gcttgtaatt taggtaaaac gtaggcaacc agttcttgct cataacgatg
     1861 aatattctcc ataccaatct cattcaagta atcgattgct cctgcaatgt tcggagtacc
     1921 agcttcaaat ttccatggca gctctttcca tgaactatcg taaagattga caaaatctat
     1981 catttcaccg ccgaattcga ctggctccat ttgttctaac aattgacgtt tgccatataa
     2041 tacaccgatt cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa aatctgcatc
     2101 caacgcttgt acatcaactg tcatatgcgg tgctgcttgg gcaccgactg gactatcctc
     2161 aactacaaca gcctgttcaa ccctcagccg tttcaagcac aacaaataac cttctggatc
     2221 tggtttgccg acctgaagat catcgatcgt cacaaaggaa tcaatttttt ccttaatcat
     2281 cggatgtgct tcaaaaaaat agtctagctc ccgcagttca gaattgagac tactccgatt
     2341 ttccagtgct gctgatccgc tgctcaaaat gttcagcatc cactggtctc ccaaccgctt
     2401 gacttatccc ttcataaaag gacagttctg ctgccgtccc aacaccttgt tgatagtctg
     2461 caaaaggaat tttatagccg aactgctcca gtaaataggc ttcagtaaat agtgatgaat
     2521 actctcgtaa aacatccatt cactgtcaat catcgtccca tcaaaatcaa aaataagtgc
     2581 tttggtcatc ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct gctctctcta
     2641 cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg tggcgctata
     2701 atttctagga ctgatagtaa atcattctat cagtcctttt gagcataaaa aaacaaaaca
     2761 tacagaagct ccgttagaat tgaatcacca caacacaatc aaaggagcgt tctgtatgcc
     2821 tgatcttcat cttatcaaac tattactagg aatcaaagat ggaaacattc aaatcactca
     2881 tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca cgctcattaa
     2941 aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga tcgttaagaa
     3001 cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga cttatcttgc
     3061 tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc tattttacgg cgaataccta
     3121 cattgttgaa aaaaactgtt ttatcgccaa acaagtacat tataaaatat tggaggaatt
     3181 gactgaaaaa caagcaatga cgacgatttc taaacattgc ggcgtttctt ggtcaaccgt
     3241 ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg aaaataaatt ggttgcctcg
     3301 ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct attctttttc
     3361 ttgtatggat ggcgatactg ggaaattact ggatattctt ccttcacgta agaagaaaga
     3421 tctcatttct tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa aaatactcgt
     3481 aacagatatg aacgcctctt atgctttgct gataaaagag tgctttcctc atgccaaact
     3541 cgttgtggat cgctttcaca tcgtcaagca cctcattcgc agttttaagg atatttgact
     3601 atgagtcatg aaaagcttcg accgaaacga tccaatacaa gccaaacact atcgacaagt
     3661 gaaggcttta tctcgacttc tgatcaaacg acaggacacg cttgtttatg ataaatggac
     3721 aaaatggcgt aattttggtt gggcctattt aaccgaaagt gaagtcgtag aacgtctact
     3781 ttctacttct gatgaactac gaatagctta tgcttactat caagaaattc ttcaagcttt
     3841 ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa acaatgccca agtcggttcc
     3901 aagagaactg catcatataa aaaaagcgtt tataaactat gaatccggca ttcgtttagc
     3961 cttagagttg ccttactcaa atggcaaaat agaaaatctg catacccaca tcaaagcatt
     4021 gaaacgaata gcttacgggt taggatgaga accagaattt tcttaatcaa tcatctgatc
     4081 acttattaaa cataaaaaaa catctaaagc agaaaatcaa ttctacctta gatgttctta
     4141 tatcactcta tcagtcctat ttgacaaaga gccagaaatg tttgccgact atacactagc
     4201 tttgttgtgc aaaatgctga gtatcaattt ccacagggat tgctagaata tctttcagct
     4261 gaatgtataa ggcatcgcta attttctctt cccgatcgtt caaccgatcc actaaaattt
     4321 ctttgccctc cagcgtttga aaaacatacc gttgaatgtt gcctaaaata aatgattcct
     4381 tccatagaac cggaatctta accccctgag gaaccggttc aaattgaatc agctccggtc
     4441 ttaaataata aatgcagcga ttgcccttag cggaaaactc gtgggtatac tgattcagct
     4501 cataccattc gaaacgatta tagttgccga taaattccgc aacaaatcgg ctctgaggat
     4561 tctgatacaa ttccttcgag gtggaaacct gagccacccg cccagcttcc atcacaaaaa
     4621 tccgatcact gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc atcgtcatcc
     4681 ctagcgattg ctggatagcg cgcaaatccc gctgcagctg ttttctaatt cgggcatcca
     4741 aagcacttaa tggctcatcc agcagcagga ctttcggttc agtgaccaat gagcgagcca
     4801 gtgccacccg ttgtcgttga ccgccagaaa gaaactggac atttttatct gcctgctccg
     4861 tcatatccac caatgatagc atctcaaaaa ccttttcctg aatcacctca ggactcagct
     4921 tctttacctt caaaccaaag gctacatttt caaaaacagt catagttgga aacaatgcat
     4981 actgttgaaa aacaaagccg atattgcgat ccttcgtagg cttatcggca atattctcat
     5041 tgtccagcac aatctcaccg ctctttggtt gttccaatcc cgtgatacag cgcagcaacg
     5101 tagactttcc gcacccgctg ggacctagta aagtaaccag ctcacccttt tcaatctgaa
     5161 tttcatcaat agctaaaatc tgtttttcat caaaaaacag attgagatca tgtatttcta
     5221 aaaacatttt ccgtcacaac tcctttaagg gcttcttttt cgactgcttc tcttccctcg
     5281 tctgcttaag ggacagctgt tgcttttgtc gataagtcag cataaaggta gttaaagcaa
     5341 ttcccaccat aaacagcaaa tacaaaacca tcacagcgct agccaaatgg ccgttttcgt
     5401 tcatccgccg cagcatatag atgcgtaccg tctgaaatcg accgccaatc aacaggttcg
     5461 tcagcatgta ctccccaaaa accgaagaaa aaatcatcag agaaactagg gtcacaccga
     5521 tacggatgtt tggaaacagc acctggataa taatcgtaga catcggggcg cccaaggttt
     5581 ctgctgcatc cactaattcc ttcagattaa ttaagcgcat ctggttgtta agactaagat
     5641 agacaatcgg caacgccgta ataaacaatg cacctaccaa cactacaaac attgggatac
     5701 cagtttcagc atacgttcga agcaaagccg ttaccaaaat cactcctggt aaagcatacg
     5761 gcagcaggag cagcttttcc agccaacgat tcaaacgagg gaaatacaaa tgaatccaaa
     5821 taatggtggg aatcatcaac aacagaatcg tcaccaagac cacacctgct aataaaagtg
     5881 agcgacccac cgcagcaata aattccctgt ccgtcagcag ctgttggaac cattgcaagg
     5941 taaaatcact aggtaaaagc gattttgacc atttagtgga gctggcatac aaaatcgttc
     6001 caataatcgg cagcaagtaa tacaggacaa agcagaacaa aatcggtttt attaactttt
     6061 tcacgtctgc accaccttgc ctttcttttg aataatcaac attgccgtta ccagcaggac
     6121 acctaaagcg actgccaacg cactgcctaa attaggcttg gcgaacacat ctcccgacac
     6181 taaagccgca attcgaatcg ttaaaagatt tacattactg ctggtcaggg catacgctgt
     6241 ttcataggtc cccatgccat tagcaaataa gatgacaaag gtgctggcaa tcgatggtcg
     6301 caaaaaaggc aaagccacct tgaaccaagc atacgctgat gaagcgccca gcaattgtgc
     6361 ggactccagc cattcctttt tcacttcctt catgctggga tacaaaaaca agacccctaa
     6421 cggaatttgg aaataggtat acgtgatggt caatccccac caagaataca aattgaagtc
     6481 cgctagaaat ggcaaaatca ttttcagcac acctacattc cccaacaaaa taataaaaga
     6541 gaatgccagc ggaatcccag caaaattagc tgccaaattg gaaacaaagg tgattttctc
     6601 ctgcaattta gtcgatagat tcattaagca taaacataaa ataaacgtcc cgattaaccc
     6661 aatcacactg gagctgacag caatcagtac gctgttaaca aaggcctgtg tgtaatattt
     6721 gttgctgaaa atatcactgt aattggccaa tgtccactgg ttagttaaat cactgtgcaa
     6781 gctgcacaga accataaaaa gcagcggcag cagtaaaaat gcacagaaga taaaggaggc
     6841 aggaaccaga accaagcgtt ttttcatctc tttcatcccc tcatcaattc ttgaatgtcc
     6901 tgcattttct ctgacggtgc aattcctaat aaccagcaaa acaatggagc cgccagcagc
     6961 tgtggtacag tttgttcctt cagcttttgg cgtttatttg aaatcaaata aaaaggaact
     7021 tcacgatgtg cagccaagct gccgccatgc agtccgtgcg catccatccc gtgatccgca
     7081 gtcaccacga cttgataacc tttttgcagc cattcaggaa ggtaaatagc caataatctg
     7141 tccgcctgat taaccgctgc tgcatattca tgagaatcgg cggtaaattt gtgaccgaca
     7201 tcatcaatat tcatcgaatg tacgaccaaa aagtctggct gatactgatt cactaaataa
     7261 tgggcatccg caaataaatg cgaatcaggg taagtgtcct cactataaaa gatgccatgc
     7321 tgaatcagtc gctcctcctg atgctggatg cgatcaacca aacgattata gggggcttga
     7381 ttgtacaact cgctgtacca gtggtaagcg gcggctcctg tttttccgcc agcttcctta
     7441 actaattcaa acagcgacca ctctttggat cgctgagcat ggtaattact atagatgcca
     7501 ttttcatagg ctggaacgcc ggtcatcagt acttcgtata acggacgtga attgctgggc
     7561 agctcacttt ttaccttcaa taagcttgct tgctggtgct caactaaatg attcatgaac
     7621 ccaagctggg cgacggctgt gtcgtagcga cagccgtcta gtataactac aatcagtttg
     7681 tcaagtctac tttgcatagg tcagtacctc cgcctgccaa agctctggaa gctctacaga
     7741 ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc gcttcataag catcctccgg
     7801 cagtaatttt tcagctactt ctttaggcag ctctaccttg cgaataggtc gcgcatagcc
     7861 ctttgccaaa ttgatttgcc cttcatcaga taaaatatat tcccgtgcta gctttgccgc
     7921 attgggatga ggggcatttt tattgattaa ggttgtatac ccgctaatga tggagccgtc
     7981 tgttggcaca gttacctcaa aacgctcgga gtcaatttgg tcacgataat ttagtccatt
     8041 gaagtcccaa acaactgcca catcgatttc acctttttca agatttgcta cgctggcatc
     8101 aacggtggac aaacggcctt tttctgccat ttttttgtag taattcagac ctggctgaat
     8161 atccttttcg tcacctccgt tggcaatcgc cgcagccaat acagcaaatt gtgcttggtt
     8221 ggcaatcgtc acatccccca cgctaacttg atagtcaccc tcttgcagct ccttccagct
     8281 agtcggtgct tgcttcacat tctctttatc ggtgataaag gaaatggttc ccgtatagct
     8341 taacaaccaa tccccatcat catcctttgc ccaatccgga atttcctccc aatagttggt
     8401 cttataaggt aaggttaact ctttgctttc agccaacgga ccgaagctga taccgacatc
     8461 accaatatcg gcggttccat tttttccttc tgattcaaac ttagccaatt cttctgcact
     8521 ggacatatcc gtatccacgt gacttaagcc gtattctttt tcaaggtcgt tccaagtgcc
     8581 tacccaattt gcccaagtat ccggcattcc caagctggca atctctcctt cttccttggc
     8641 tttttctata atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg ttgcttcacc
     8701 tgagccgcag cctgtcagca ggatacttac tgttgctgca caaaataaac cgatagtcat
     8761 gttacgaatt ttttcattca ttttcattca ttcttctccc ctttcgataa ttccattcta
     8821 acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca atgtaaaggt
     8881 tgtatatggt ttgtaaattt cgacgaattc ccgttaacct tctaaagctt ttgcggtatg
     8941 atagtagaaa aaaggaggct ccctatgcag tattatgatc agcatctcca cacgtatttt
     9001 tcctttgatt cagaggaaaa atttgaaaac tatttagcct atcagccgga gtttttcgtt
     9061 tctacggatc actttgattt aaacaatcca tctaccggaa aagatgacat tccggattat
     9121 ccagcctata taaacaaact ggactcttta aaagaaacct attccaccaa atttctgcga
     9181 ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga tcgtggatta cctcgctcag
     9241 catccttatg atttaaagct ggtaagtatt catcaaaatg gccgctttga ttatatggac
     9301 gatgtggttt taaccaaaga tcccattcaa actaccaagg aatattttga tcagatgttg
     9361 caggtcttga cggattttcc agaaggagat attttgactc actttgatta tggattacgc
     9421 cgcttcacct tcacagcgga agaattcgcg gagcattttg aggctcagct gctaacgatt
     9481 ttcaaaaagg ttgttgaacg agaattggcg atggagttaa atgccaagag cttcttgaaa
     9541 tacggcaacg aagaccttta ccgttatgcc gttcctttgt atcaaagtgt cggcggaaag
     9601 ctctttacct taggctcgga tgctcatgtt gccgaagatt atcagctggg ctttaaaaaa
     9661 atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc tagtcattca aggaaaagaa
     9721 cgccgcctga cgccgctgcc gattttgtga ttcctatata atagaagaaa gtggtgaaaa
     9781 aaatgagcaa cctgtgggac tacaatcaag aggcacccat ccattattta attgcgcgac
     9841 attgggatgc gttgaaaatt gaggcggttt gccgatcctt gttagcagcc gttccaaaac
     9901 aacaactgga aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt caggcttatt
     9961 ttgcagcctt taaagatcag cctttggaat atttgcatgc gcagtttcat cttttttatc
    10021 aagtagccgc cccagatgac tacaacgatt tacgaggaca gctgcaattg acctttcaag
    10081 cggatgaaac cgcctatacc gttttattgg gaatggctcg ccttggtgat caggcgaagg
    10141 tcgagtggcg gattttcgat atttgatggc aggatcaagg cgtttcagtt gcaaaccttc
    10201 ctctctttca attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc accgatgctg
    10261 gtgaaattcg tctccgctta tttcctgacc aagctcccct ggctgtagcc aactggcgct
    10321 ccctagctga gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat taaggaattt
    10381 gttattcaag gtggcgcttt ggatggttct ggtgatgaag ctgtttccag ctatgagggc
    10441 tttttcgctg atgaagtgca tcgcggactt tatcatttta atggcgcctt ggccttaggg
    10501 gaccacggcc cccacaccaa cggcaaccag ttttttattg tgcaaaacac aaaggcgcag
    10561 gctgacctgc taatgtaaag caagcatact tggagcttgg aggactaccg gagctggatg
    10621 gtcgttatac tgtttttgga caggtatacc aagggttaga aatcgtagca gcgatcgcag
    10681 ctcagccgac aaacagtgaa gatgtaccga ttgagccagt gttcattcga caaattaact
    10741 tcgaaaaaac atcgtaaaaa gacactcact gacttcagca aaagctctat tcttgagctg
    10801 gctgcagcta gtgagtgtct ttcatttatc cataagataa agcttggatt caataaataa
    10861 ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc cagttgatct
    10921 gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg atactccagt
    10981 cccaatgctt ggtaaccgtg cataccatag cagtggtacg gtagtaattc taacggtacg
    11041 aaccgatcaa gatctgccac aaaacaagca atcgtcagca gctcggtcag gttcatattg
    11101 atacctggaa ccacaggaac tcgaattcgc acttcgcagt taggatactc aattaaaaag
    11161 cgacgaatat tcttcaaaat ttgttggtta tctaagccgg tccattgttt gtggagcttg
    11221 ctggtaaaca tttttacatc cacaaacaat gtatccacat agggagctac cttttggatt
    11281 tcattataag caccacaaaa gctggtttcc agtacggtat tgattccaat gtatttcgat
    11341 ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc gctaatggtc
    11401 actccgccat ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc cattacctct
    11461 tcagcggtca tggtctggcc aaatcctttt tcaatctgct tgctttgtga ttcgggaatc
    11521 gaacaccagg cacagcgtaa tgggcagccc tttaaataaa caacggtccg taaaccagca
    11581 ccatcatgta gagaaccctg ttctattctt aatactgttc cttccatata gatttttgaa
    11641 ttacacacta gtttaaacta aaacacacaa ggaaaaactt accatttaat cataaaatta
    11701 gctcatataa atatactagg atgtaaaata gactggtcaa aatctggtca aaaaagagga
    11761 ctaatacagg tagtcctctt ttttgttatt ttaatattct aaatcatttt ttattggttt
    11821 attcccttat tcgatgtaga ctcaaatcac tatatcacta gatatcaaac ggcgtcatac
    11881 ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt gaataacatt caaaatcaat
    11941 aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa aaaatatata cagccttgta
    12001 ggtaaagtac cacagggctg tatatttttt atttcagatt accttaaatg attccattta
    12061 ctttttatct cattttttcc tactagttag tatcaaatac tctcctattt tctcaataaa
    12121 ctatttttgg atacatggta tctgaaacag ctaaaacctt gtcattacag caaaaaatca
    12181 cgttatccag actgtagaca aagtccattt tatggatgat gtctacagt
//
LOCUS       DADQRX010000078        12222 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.080, whole genome shotgun sequence.
ACCESSION   DADQRX010000078 DADQRX010000000
VERSION     DADQRX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.080"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..1106)
                     /locus_tag="J8V14_002097"
     CDS             complement(324..1106)
                     /locus_tag="J8V14_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922191.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="J8V14_002098"
     CDS             complement(1171..2007)
                     /locus_tag="J8V14_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922192.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="J8V14_002099"
     CDS             complement(2025..3434)
                     /locus_tag="J8V14_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0922193.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="J8V14_002100"
     CDS             complement(3448..5271)
                     /locus_tag="J8V14_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0922194.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6114..6440)
                     /locus_tag="J8V14_002101"
     CDS             complement(6114..6440)
                     /locus_tag="J8V14_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922195.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6486..6848)
                     /locus_tag="J8V14_002102"
     CDS             complement(6486..6848)
                     /locus_tag="J8V14_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBA0922196.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6885..7099)
                     /locus_tag="J8V14_002103"
                     /pseudo
     CDS             complement(6885..7099)
                     /locus_tag="J8V14_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7227..7562
                     /locus_tag="J8V14_002104"
     CDS             7227..7562
                     /locus_tag="J8V14_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922197.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7638..9635)
                     /locus_tag="J8V14_002105"
     CDS             complement(7638..9635)
                     /locus_tag="J8V14_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922198.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9733..10008)
                     /locus_tag="J8V14_002106"
     CDS             complement(9733..10008)
                     /locus_tag="J8V14_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0922199.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10240..10974
                     /locus_tag="J8V14_002107"
     CDS             10240..10974
                     /locus_tag="J8V14_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922200.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11013..12155
                     /locus_tag="J8V14_002108"
     CDS             11013..12155
                     /locus_tag="J8V14_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0922201.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3974 a         2430 c         1857 g         3961 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
     6061 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
     6121 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
     6181 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
     6241 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
     6301 tgtaaaatct gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt
     6361 tttgaaaaaa tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg
     6421 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
     6481 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
     6541 atcatcaaaa tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca
     6601 gcaacagcac caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca
     6661 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     6721 attgtctgct cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt
     6781 agtgctccgc aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
     6841 caatacatgt cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta
     6901 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
     6961 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     7021 acattcattt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg
     7201 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
     7261 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
     7321 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg
     7381 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
     7441 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
     7501 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     7561 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     7621 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
     7681 ccatcatctc ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat
     7741 atcaattctt ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta
     7801 ctacttgctt tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat
     7861 ccttgtttaa caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca
     7921 acattacgta gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt
     7981 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
     8041 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
     8101 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
     8161 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
     8221 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
     8281 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
     8341 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
     8401 aaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
     8461 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
     8521 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
     8581 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
     8641 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
     8701 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
     8761 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
     8821 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
     8881 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
     8941 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
     9001 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
     9061 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
     9121 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
     9181 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
     9241 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
     9301 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
     9361 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
     9421 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
     9481 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
     9541 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttctttatct
     9601 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
     9661 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
     9721 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
     9781 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
     9841 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
     9901 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
     9961 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    10021 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    10081 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    10141 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
    10201 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    10261 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    10321 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    10381 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    10441 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    10501 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    10561 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    10621 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    10681 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    10741 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    10801 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    10861 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    10921 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    10981 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
    11041 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    11101 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    11161 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    11221 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    11281 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    11341 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    11401 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    11461 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    11521 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    11581 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    11641 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    11701 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    11761 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    11821 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    11881 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    11941 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    12001 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    12061 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    12121 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    12181 tctagtatac aggcaaaaca aaaaagctag aacccttata ta
//
LOCUS       DADQRX010000079        12210 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.081, whole genome shotgun sequence.
ACCESSION   DADQRX010000079 DADQRX010000000
VERSION     DADQRX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.081"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="J8V14_002109"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="J8V14_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBA0922202.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /locus_tag="J8V14_002110"
     CDS             complement(652..1374)
                     /locus_tag="J8V14_002110"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBA0922203.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /locus_tag="J8V14_002111"
     CDS             complement(1510..2391)
                     /locus_tag="J8V14_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBA0922204.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="J8V14_002112"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="J8V14_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBA0922205.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="J8V14_002113"
     CDS             3631..4110
                     /locus_tag="J8V14_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBA0922206.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="J8V14_002114"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="J8V14_002114"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBA0922207.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="J8V14_002115"
     CDS             5411..5539
                     /locus_tag="J8V14_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBA0922208.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="J8V14_002116"
     CDS             5543..6088
                     /locus_tag="J8V14_002116"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBA0922209.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /locus_tag="J8V14_002117"
     CDS             complement(6542..8233)
                     /locus_tag="J8V14_002117"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBA0922210.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="J8V14_002118"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="J8V14_002118"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA0922211.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="J8V14_002119"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="J8V14_002119"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA0922212.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="J8V14_002120"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="J8V14_002120"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBA0922213.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3344 a         2530 c         2203 g         4133 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg taatttattc
//
LOCUS       DADQRX010000080        12034 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.082, whole genome shotgun sequence.
ACCESSION   DADQRX010000080 DADQRX010000000
VERSION     DADQRX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.082"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="J8V14_002121"
     CDS             140..460
                     /locus_tag="J8V14_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922214.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="J8V14_002122"
     CDS             912..1112
                     /locus_tag="J8V14_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA0922215.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="J8V14_002123"
     CDS             1259..4048
                     /locus_tag="J8V14_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBA0922216.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="J8V14_002124"
     CDS             4095..4622
                     /locus_tag="J8V14_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBA0922217.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="J8V14_002125"
     CDS             4643..5833
                     /locus_tag="J8V14_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA0922218.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="J8V14_002126"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="J8V14_002126"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBA0922219.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="J8V14_002127"
     CDS             7966..8640
                     /locus_tag="J8V14_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBA0922220.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="J8V14_002128"
     CDS             8637..8978
                     /locus_tag="J8V14_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922221.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="J8V14_002129"
     CDS             9008..9367
                     /locus_tag="J8V14_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBA0922222.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9561..9749
                     /locus_tag="J8V14_002130"
                     /pseudo
     CDS             9561..9749
                     /locus_tag="J8V14_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="J8V14_002131"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="J8V14_002131"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBA0922223.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4008 a         2108 c         2593 g         3325 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaat
//
LOCUS       DADQRX010000081        12015 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.083, whole genome shotgun sequence.
ACCESSION   DADQRX010000081 DADQRX010000000
VERSION     DADQRX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.083"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..643)
                     /locus_tag="J8V14_002132"
     CDS             complement(62..643)
                     /locus_tag="J8V14_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0922224.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(826..1452)
                     /locus_tag="J8V14_002133"
     CDS             complement(826..1452)
                     /locus_tag="J8V14_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA0922225.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1474..2805)
                     /locus_tag="J8V14_002134"
     CDS             complement(1474..2805)
                     /locus_tag="J8V14_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBA0922226.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2960..3361)
                     /locus_tag="J8V14_002135"
     CDS             complement(2960..3361)
                     /locus_tag="J8V14_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBA0922227.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3447..3653)
                     /locus_tag="J8V14_002136"
     CDS             complement(3447..3653)
                     /locus_tag="J8V14_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922228.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3748..5250)
                     /locus_tag="J8V14_002137"
     CDS             complement(3748..5250)
                     /locus_tag="J8V14_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBA0922229.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5268..5723)
                     /gene="argR"
                     /locus_tag="J8V14_002138"
     CDS             complement(5268..5723)
                     /gene="argR"
                     /locus_tag="J8V14_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBA0922230.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5821..6438)
                     /locus_tag="J8V14_002139"
     CDS             complement(5821..6438)
                     /locus_tag="J8V14_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922231.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6659..8032
                     /locus_tag="J8V14_002140"
     CDS             6659..8032
                     /locus_tag="J8V14_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBA0922232.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8107..9219
                     /locus_tag="J8V14_002141"
     CDS             8107..9219
                     /locus_tag="J8V14_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA0922233.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9291..10928)
                     /locus_tag="J8V14_002142"
     CDS             complement(9291..10928)
                     /locus_tag="J8V14_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBA0922234.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11096..11704)
                     /locus_tag="J8V14_002143"
     CDS             complement(11096..11704)
                     /locus_tag="J8V14_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBA0922235.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3196 a         2513 c         2151 g         4155 t
ORIGIN      
        1 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt
       61 attaaagaac cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa
      121 gtagtgatag atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca
      181 acgctttggc ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga
      241 tattttgttt cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt
      301 cttcatccac tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga
      361 tctgtgtctc atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct
      421 gttttccagc atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat
      481 ggatcaccgc tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc
      541 ctgtatcaga agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa
      601 taagatcttt ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc
      661 atttctcata gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa
      721 aaagactgtg ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag
      781 atgttcattg attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc
      841 taatacttcc tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc
      901 atccattgct tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc
      961 actctctacc aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag
     1021 aattactgtg tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc
     1081 aaagatttta tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc
     1141 gataaaatcg gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat
     1201 ctgctgctca agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg
     1261 gtaaaaataa tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat
     1321 ttcaatcata ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc
     1381 accgcctgct ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt
     1441 attattgaac atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc
     1501 actcattgtc ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt
     1561 catagctagc gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc
     1621 tagaatcaag tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa
     1681 tacttgtgct ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg
     1741 tgctttggct tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact
     1801 gtaaggcaca gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat
     1861 aatgattcga taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc
     1921 aatcgcccaa atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg
     1981 atctgttagt ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc
     2041 agttgcagcc aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt
     2101 gacggcagct gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc
     2161 gattcccgca tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg
     2221 aagaaattca gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga
     2281 tgcaaattcc aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc
     2341 ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg
     2401 taaaatgatt ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt
     2461 ttcaccatta ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat
     2521 cactgtccca gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt
     2581 caatttttct ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat
     2641 caatgattta gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga
     2701 cttttcaacg actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc
     2761 gccctttcca aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct
     2821 tttttaccta ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca
     2881 tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg
     2941 ttcgatttcc tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg
     3001 ctttggagta agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc
     3061 tgatgccatc gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat
     3121 tataaagaga gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca
     3181 tatgttgtac cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat
     3241 ttgttccttc tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg
     3301 cctcgacttc tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca
     3361 ttgtactgct cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag
     3421 taaggcgctt tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt
     3481 tttccaaaaa acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa
     3541 atagcaatct tttctacata atcttttata tgattgattt taagatacat atataccaga
     3601 aagttgaccc cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc
     3661 tccttttgct ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca
     3721 agcaactagc ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa
     3781 atgtccgaat aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt
     3841 ttctatctgt aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt
     3901 tccgtatggc tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat
     3961 ccctttttct tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg
     4021 tggttcatcg ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc
     4081 tttgatttgt tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat
     4141 ctttgcgatg acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc
     4201 gcttgtttcg atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt
     4261 gattacattt cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa
     4321 tctttgtaga tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt
     4381 taatacgatt gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc
     4441 actgacaact gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt
     4501 tgctaaaaca gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg
     4561 attaggaaat cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct
     4621 attcaatact ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt
     4681 ccaaccattt agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa
     4741 atcttgcgca taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt
     4801 tattttttgc tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga
     4861 ggtaaagaat tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc
     4921 agcatcgcac gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa
     4981 ttcgaaatcg atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag
     5041 tgctggtccg cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc
     5101 ggttgatttc ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat
     5161 gtctgtcgtc aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga
     5221 tccattttgc agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt
     5281 ttttccagct tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat
     5341 gtatctacac cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc
     5401 gttaccacat cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg
     5461 atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat
     5521 ggctggttaa ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc
     5581 atttctcgaa tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc
     5641 cgcgcaagca gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt
     5701 gcttgtcgct gtttcttttt catattagac acctctcaat gttgattata gcaaaagata
     5761 agtaatattt cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg
     5821 ctaacctttt tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct
     5881 tggatcgctg atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc
     5941 gatgaatgct tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa
     6001 gatttgggca caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc
     6061 cggaagtttg ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg
     6121 ctgtcggatc atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata
     6181 cttttcttgc agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg
     6241 aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc
     6301 tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga
     6361 ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg
     6421 ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat
     6481 acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat
     6541 aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt
     6601 ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat
     6661 gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt
     6721 tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt
     6781 tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat
     6841 cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc
     6901 ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt
     6961 cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt
     7021 ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac
     7081 tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca
     7141 ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat
     7201 cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag
     7261 cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac
     7321 agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg
     7381 aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg
     7441 cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt
     7501 gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct
     7561 tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata
     7621 tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt
     7681 attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat
     7741 gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga
     7801 agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt
     7861 ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta
     7921 tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct
     7981 cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata
     8041 tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc
     8101 ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt
     8161 tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact
     8221 aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat
     8281 cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat
     8341 caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa
     8401 ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg
     8461 ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt
     8521 atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta
     8581 gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct
     8641 tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc
     8701 ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa
     8761 gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg
     8821 cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc
     8881 catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact
     8941 aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc
     9001 cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat
     9061 gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat
     9121 atcggctatc tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa
     9181 aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa
     9241 ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat
     9301 ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc
     9361 tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac
     9421 gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga
     9481 ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt
     9541 tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc
     9601 tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa
     9661 ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc
     9721 gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg
     9781 atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt
     9841 tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt
     9901 ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc
     9961 acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt
    10021 ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa
    10081 gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt
    10141 gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca
    10201 tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca
    10261 agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta
    10321 ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga
    10381 tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag
    10441 atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct
    10501 tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt
    10561 gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat
    10621 ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca
    10681 atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca
    10741 tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct
    10801 gtcttcctat caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat
    10861 cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt
    10921 gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt
    10981 cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa
    11041 aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt
    11101 accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta
    11161 catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag
    11221 catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt
    11281 ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg
    11341 cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg
    11401 tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat
    11461 ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat
    11521 ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag
    11581 ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta
    11641 caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat
    11701 tcatattaaa ctcctttttt tctccgtttt ttattctttc gacgctatta actttatcat
    11761 ttagaaatat ttcataggta aaagaaacat gacatttaat gacaaaataa cgaattattt
    11821 aatatttgta acatttaaac aaatattttt ttgatacaaa accataaaag ccttgttgta
    11881 taagcaaagt aatcgttttt ctatgttttt ttatttttga atgaaaaacg cagattctgt
    11941 aacattcact gcaataatcc ataaaaaaaa gcaaaaaaat ttttgatttt tatcaaattc
    12001 tcttaaaaaa atgta
//
LOCUS       DADQRX010000082        11659 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.084, whole genome shotgun sequence.
ACCESSION   DADQRX010000082 DADQRX010000000
VERSION     DADQRX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.084"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            300..1343
                     /gene="pheS"
                     /locus_tag="J8V14_002144"
     CDS             300..1343
                     /gene="pheS"
                     /locus_tag="J8V14_002144"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBA0922236.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            1348..3768
                     /locus_tag="J8V14_002145"
     CDS             1348..3768
                     /locus_tag="J8V14_002145"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBA0922237.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(3938..4948)
                     /gene="trpS"
                     /locus_tag="J8V14_002146"
     CDS             complement(3938..4948)
                     /gene="trpS"
                     /locus_tag="J8V14_002146"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBA0922238.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            5202..5939
                     /locus_tag="J8V14_002147"
     CDS             5202..5939
                     /locus_tag="J8V14_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA0922239.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            5953..6768
                     /locus_tag="J8V14_002148"
     CDS             5953..6768
                     /locus_tag="J8V14_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBA0922240.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            6781..7428
                     /locus_tag="J8V14_002149"
     CDS             6781..7428
                     /locus_tag="J8V14_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA0922241.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            7444..8100
                     /locus_tag="J8V14_002150"
     CDS             7444..8100
                     /locus_tag="J8V14_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA0922242.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            8262..9083
                     /gene="racE"
                     /locus_tag="J8V14_002151"
     CDS             8262..9083
                     /gene="racE"
                     /locus_tag="J8V14_002151"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBA0922243.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            9086..10441
                     /gene="rph"
                     /locus_tag="J8V14_002152"
     CDS             9086..10441
                     /gene="rph"
                     /locus_tag="J8V14_002152"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBA0922244.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            10442..10960
                     /locus_tag="J8V14_002153"
     CDS             10442..10960
                     /locus_tag="J8V14_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBA0922245.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            11067..11567
                     /locus_tag="J8V14_002154"
     CDS             11067..11567
                     /locus_tag="J8V14_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBA0922246.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         3850 a         2006 c         2535 g         3268 t
ORIGIN      
        1 aaaaatcttg taaactatag tccatacgaa taagtaaaga acgatgacga aaaagagtag
       61 tttggctaaa ccagttcagg gagatctgtc gcagactgaa agcagatcct gtgtttgaaa
      121 aatgaagttc actttccgag ttcgtctgat aaaatcagac cggcaattgt cgttaacgaa
      181 gtaagagtgc ggtgtaagtc atttatccgc aaattaggat ggtaacgcga agacctcgtt
      241 ccttaacagg aatgaggtct ttttgttttg tatttggaat ttaaaggagg aacaagcaca
      301 tgtctctaca agcacaatta gagagcttga aagaagcaac gctcgtcaaa atcacagcgg
      361 cacaagattt aaatgaatta aatcaaatac gtgttgaaac gttaggaaaa aaaggaccga
      421 tcacagaagt attacgtggg atgaaagatc taagtccaga agagcgacca aaagtcggaa
      481 gtttcgcaaa tgagatccga gatcttttga cagaagagat cgagcgaaaa aaacaagtat
      541 tagaagaaga agcgttaaat gcagctttgg caaaagaaac aatagacgtg acactacctg
      601 gtagaaaagt aaaacatggt acacgtcata tcgtgacaca aatcatggaa gaaattgaag
      661 atatttttgt gggtatgggc tatcagatcg tagaaggata tgaagtagaa tctgatcatt
      721 acaatttcga acggatgaat ctgccaaaag atcatccagc aagggacatg caagatactt
      781 tctatatttc agacgagata ttgattcgga cacatacttc accagtccaa gcacggacaa
      841 tggaaaaaca tgatttctct aagggtgctc tacgaatgat ctcgccaggg aaagtcttcc
      901 gcagagatac agatgatgcg acccacagtc atcagttcca tcaaattgaa gggctagttg
      961 ttgataaaaa catcacaatg ggcgacctta aaggaacatt agaagtcgta atgaaaaaaa
     1021 tgtttgggga agatcgtgaa attcgtttgc gtccaagtta tttcccattt acggaaccat
     1081 cagtagaagt agatgtcagc tgtttcaaat gtggtggtgc cggttgtaac gtatgtaaat
     1141 acactggatg gatcgaaatt ttaggagctg gcatggtaca cccgaatgtg ttgaagatgt
     1201 caggaatcga tccagaagaa tattcaggct ttgctttcgg ccttggacca gaccgcgtag
     1261 ctatgttgcg gtatggagtg aatgatatcc gtaatttcta tcaaaatgac ttacgtttct
     1321 taagtcagtt caagggggag taagaaaatg ttagtttcat ataaatggct caatcgttat
     1381 gtagatttat cgaatgtaac accaaaagaa ttagcggata agatgtctgt gacaggaatc
     1441 gaagtagagg gagtaacggt tccagaagaa ggcttgaaaa aaatcgttgt cggtgaagtc
     1501 aaggaatgcg taccacatcc agattctgat cacctttcta tttgtcaagt cgatattggc
     1561 gaagacgaac tgtctcaaat cgtttgtgga gcgcctaatg tgaaggttgg aatcaaagtg
     1621 attgttgcac ttcctaactc acgtatcgct ggtaatgtaa aaatcaaaaa aggaaaaatg
     1681 cgtggccaag tatcaaatgg catgatttgc tcattacaag aaattggcta ttcagatagt
     1741 gttataccga aagaatacgc tgaaggtatc tattatatgc cacaagaagc tgtaaatggc
     1801 gatccagttt ttccttattt ggatatggat gattcgatta ttgaattatc tatcacaccg
     1861 aatcgtgcgg atgcattgtc aatgcgtggc gtagcttatg aagttggggc aatttatcgt
     1921 cagactccta cattcgatga cgaaaagctg gtagaatcct cacgtcctgc tagcgataaa
     1981 attcaagtgg aagttgaaga taaagaagca gttcctgctt atcaaatcag aatcattgaa
     2041 ggagtaagaa tccagccaag tcctcaatgg ctgcaaaatc ttttgatgaa cgaaggcatt
     2101 cgtccaatca acaatgtagt agatgtgacg aactatattt tgcttttatt cggacaacca
     2161 ctacatgcat ttgactacga caaattgaaa tcaaaagaga tctttgtccg ccgtgcaaaa
     2221 gaaaatgaaa cattagtaac actagatgga gaatctcggg aattgacaac tgacaatctc
     2281 gtcattacta acggcaaaga accagttgct ttagctggtg tcatgggagg gttagactca
     2341 gagatcacga atgaaacaac aacagttgca ctagaatctg ctttatttga tccaatttcg
     2401 attcgtcgta cagcaaaaca atttaatttg cgtagtgaat cctctgcacg ttttgaaaaa
     2461 ggaatcaacc atgcgactgt tggagaagcc agtgaggttg ctgcagcaat gatcgtttct
     2521 cttgcgggtg gggaagttct acaaggcgcg gtcaaaggct cagagatcaa agcagaagac
     2581 gtatcagtga agatttcact tgaacgcatc aatcaatatt taggaacatc tcttgtcgtt
     2641 aaagaagtaa atgagatttt tgaagcactt ggatttagct atgaagaaaa ggagtatgtt
     2701 tattccgtag tagtaccacc aagacgctgg gatatccaaa tcgaagcaga tattatcgaa
     2761 gaagttgctc gtatttatgg atacgaccag ttgccatcta cacttccaag tggagaaacg
     2821 gttgctggaa gcttgtcaaa agaacaagaa actactcgta aaattcgtac aatctttgaa
     2881 ggaagcggat tgagcgaagc aatcagttat gcattgacaa cagaagaaaa atcaagacag
     2941 tttgttataa atgaaagtaa tattacacgt ttggactggc caatgagcga agaacgttct
     3001 gtattacgaa tgaatttgat cagcggacta ttagatgata tcagttataa cactgcccga
     3061 aaaaatacag agattgcttt atatgaaatt ggtcgggtgt tcttccaaga aaatgatcca
     3121 cgtacacatc tgccgcaaga agtaaaacat gcagcaatcg ctatttctgg tatttgggaa
     3181 gaaaaagatt ggcagacgaa agctcaaaat gtagactttt tcaccatcaa gggtctttta
     3241 gaaaacttat ttgaacaact aggcatcact gaagatgtcc attatcaaac agtgacagaa
     3301 atgaaggaaa tgcatcctgg aagaactgct gcgatttacc ttggcgaaaa atttattggt
     3361 tttgtcggtc aagtgcatcc aacgattaca aaaagttatg atattccaga aacatatgtt
     3421 gctgagtttg atttatctgc tatagtggaa gctgctcaaa aagggatcgt tttcgaagca
     3481 gtgacaaaat tcccagcagt cagccgggac atcgctttgc ttgtaaaaga aactgtgaca
     3541 aatcaggaat taacagaagt tatccgttct gctgccggaa gatttttaac agatatccaa
     3601 ttatttgatg tctatcaagg agaaaacatt gaaaaaggac ataaatccat ggcgtatagc
     3661 ctgacattcg tcaacccaga agcaacattg acagatgaag aaatcaataa agcaatggaa
     3721 aaagtcgaaa aagcgttggt agaaaaccta gaggcaagta ttcgctaaga aggaaaatat
     3781 agtaagtaac gatcaagtag cttttgaaga ccatacattt gaaaaaaaga tgtgacacaa
     3841 cttcgtcaca tctctttttt tgattttaaa aaaggaatag cacagagacg agataaaaaa
     3901 acaggccttt acagcctgct tttttagagt aaatgaatta atggaagtaa ttgattccca
     3961 ttgctgattt tacttcagaa agtgtttgtg cagctacttt agcagcttct tcactacctt
     4021 tacgtaagat ttccataaca gctgaaggat ctttttccaa ttcttctcgg cgcgcacgga
     4081 tcggtgcaaa ttcagcttcc aatacatcaa tgaggtatcg tttgatcttt acatcaccaa
     4141 gtccgccgcg gcggtaatga gctttcattt cttcaattgc ttctttatct gttccgaata
     4201 catccaagta ggtgaagacc atattccctt ctacttgtcc aggatcttgt acatggatgt
     4261 ggtttggatc agtatacata ctcatgactt ttttctgtaa gacatcggca ggatcggaga
     4321 tataaatacc gtttcctaac gatttactca ttttgccatt tccatcgatt ccaggtaatc
     4381 tacccattcc ttttggtggg aatacgcctt ttggttccac caagacttca ccatatgtat
     4441 gattgaagct ttgtacgatt tcctgtgtct gctctagcat tggtttttga tcctcaccaa
     4501 caggtacaag attcgctttg aatgcagtga tatctgctgc ttgagaaaca ggatagataa
     4561 agaatcctgt aggcacgctt tcaccgaatt ttttttgttc gatttctgtt ttgacagtcg
     4621 gattgcgccg aacgcgtcct acgctgacta agttcaaata atacatggtt aattcagcta
     4681 attccggaat ttgtgattgg ataaataaag tagattttgc aggatctaat cccacggcta
     4741 aatagtctaa agcaacttgc aagacatttg atgaaacctt ctcaggattt ttagcattat
     4801 ctgtcagtgc ttgcatgtca gcaatcatga caaacagttg gttgtcttca tctgcctgca
     4861 tagccactcg ggtttttaga gaaccgacgt aatgccctaa atgcagcttc ccagttggtc
     4921 gatctcccgt taaaataata tttttcaaaa aagacacctc tttacgattt gtaagtctga
     4981 gcacttaata tcttaccgtt tttcctgaaa agaaacaaca gttcctaagg aaaaaacaag
     5041 gaaagaggat aaaataacta atagattaga aatggaagaa atatttatgg gagaataaac
     5101 gttctaagaa actttacaaa ttactaaact atcagatata atgtatttta ttatacatct
     5161 ttaaaagaaa tcttactaaa taataatggg agtgtaattc tatggcaatg attaaatttg
     5221 atcacgtgga taaatattat ggcaagttcc acgcattaaa aaatatcaat ctcgaattcg
     5281 agaaaggaga agttgtcgtt gtcattggtc cttctggttc aggaaagagc acgatgcttc
     5341 gctgtatcaa tggattagag acgatctctt caggtaagct gctgattaat gatactgact
     5401 tgcatgataa aaagacaaaa ctgacagaag tccgaaaaaa catcggcatg gttttccaac
     5461 atttcaattt gtacccaaat aaaactgttc tggaaaacat caaattagca ccaatcaaag
     5521 tgttgaagca agaccaggca actgcagtca aaaacgcgga gaaatttttg aaaactgtca
     5581 atatgttaga taagaaagat tcttatcctt ccatgttatc cgggggacaa cagcaacggg
     5641 tggcaatcgc cagaggttta gcgatgaatc ctgagatgct gctgtttgat gaacctactt
     5701 ctgctttgga tccggaaatg attggtgatg tattagacgt tatgaaaaaa ttggctcatg
     5761 acggtatgtc aatgattgtc gtgacacatg aaatgggctt tgccaaagaa gtggcggatc
     5821 gtgttatctt catggctgat ggagaagtat tggaagacag cagaaatgtg agagactttt
     5881 tcgaaaatcc aaatgaagtc cgagcacagc aatttatcag taaagttatc aatcattgat
     5941 aggaggaaaa agatgcgcag gttaaaaaaa atacgcctga ttttcctttt aggcattgct
     6001 tttcttgcac tagccggatg taaaggaaat gggttggcaa atgaagatat tttagagcga
     6061 agcaaatcta ccaatgaaat catctggggt gtcaagtatg atactcgatt atttggaatg
     6121 atggatattg agagcagaac tgtacaagga tttgatgtag atattgcaaa agcaatcact
     6181 aaaaagatcc ttggtgacaa tggaaaaaca gaatttgttg aagtcacttc taaaactcgg
     6241 attccattat tgaaaaacgg aaatatcgat gcaattattg caacgatgac gatcacagat
     6301 gaacgaaaaa aacaggttga cttttcagat gtctatttcg atgctggcca agctttgttg
     6361 gtaaagaaag gaagccaaat caaaagtgta gatgatttga atgcttctac aacggttcta
     6421 gcagtcaaag gttctacatc ggctgcgaat atccgacagc atgcaccaga tgcaaaaatt
     6481 ttagaattag aaaactatgc agaagcattt actgctttac aatctggtca gggagacgcg
     6541 atgactacgg ataatgcgat tttgttaggg attgcagatg aaaatccaga gtatgagtta
     6601 gttggtggaa cgtttactaa tgagccttac gggattgcga tcaacaaagg gcaagaaaac
     6661 ttcttgaaag ctgttaacca agcgctagag gaaatgcatg cagatggcac atacgataaa
     6721 atctatcaaa aatggtttcc aaatgaaact gaaggaaaag tagagtaagg gggagaggca
     6781 atgagcgaat tgatacaaac ttatggacca atctttgtcg acgggttcaa gatcacattg
     6841 ttttcgagtg tcttagcact tgtgtttagt ttgattatcg gaacgttaat ggcaatcttc
     6901 caattatcaa agaataaagt agtgagcgcc ttagcaaagg catatgtaga attttttaga
     6961 aacattccat tattaatcat cgtgatgttt ttttatgttg tcgtaccgat ttattggttt
     7021 aatctagatg gattccaagc aggtacgatt ggattaacga tctatacatc tgcttttatt
     7081 gctgaaacag tacgttcagg gattcaaacc gtaccaaaag gtcaaacaga agcaggacta
     7141 tcttctggtt ttacctattc agaaatcatg cgttatattg ttttaccgca agcattcaaa
     7201 atcgttatcc cgccattagg caaccaattt atcaacctcg taaaaaactc atctatttta
     7261 gcaatcgtag ctggtttaga tttgatgtat caaggagact tgatcgctag tgaaacattc
     7321 aataccttcg atacgtatat catcgttggg ttattttact tagttatcac gctaccgtta
     7381 tcttacttaa tggtttatct tgagaaaaaa tgggcggttc gttcatagaa ggaggggaaa
     7441 tacatggatt ttagcggcgc tttttcatgg atgaatatcc gttttttatt ggaaggatta
     7501 aaggttactg ttgaagtttc attgttttcg atcattttta gtttcttgat cggtggtctg
     7561 acaggtgtga ttcgttttgc gaatatccct ttcttttcca aaatccttgg tgtagtgatt
     7621 gatattatac ggaatcttcc attattattg atcattttct tcacttactt tgccttacct
     7681 caaatcggca ttcagatgaa catcttctgg tccgctgtcg cagcattaac gatttttgag
     7741 tcagctatgt tgtcggaaat tttccgagct ggactcaatg ctgttccaaa aggacagatg
     7801 gaagcaggat tatcaacggg actgacttat gtagaatcga tgcgaacgat cgtgttaccg
     7861 caagcattta aatcaatgat cccggcaatt gtgagccaat tgatttcact gatcaaagat
     7921 acttcattag cagtaatcat ttctttacct gaactttctc atcaggcacg tattatttat
     7981 ggacagaaca ccaattatgt gcttccgatg ttcatcatca tgacacttat gtacttcgtg
     8041 gtatgttacg cgctttctct gctttccggt tacttggaaa agagacggta cagttattaa
     8101 tagatgattc ttttagcggt tgtggcaaac taaagtcaca accgcttttc ctctttccat
     8161 tcgaaaaaaa gagatttcga ttgttttaag caagtaagag cacttgaaaa ttcacgctaa
     8221 aaagtacaca atagggagga tagaatttat tgaaaaaaag gatgatacga ttgacagata
     8281 atcgccctat cggatttatt gattcaggtg tcggcggctt gactgtagta aaagaagccc
     8341 tgaaacaatt accgaatgaa aatattttat ttgtaggaga cacagcacgc tgcccatatg
     8401 gccctagacc cgcggaacag gtaatccagt atacttggga aatgacggat tatctggtgg
     8461 agcaaggaat caagatgctg gtgatcgcct gcaataccgc aactgcggtg gctttagaag
     8521 aaatcaaagc tgctctttct attccagtca tcggtgtgat ccttcccggt actagagcgg
     8581 cagtaaaaaa aacacaaaat aaacaagttg gcattatcgg tacgattggt acggtaaaaa
     8641 gtcaagctta tgaaaaagca ctgaaagaga aagtaccaga attgactgtg acaagtcttg
     8701 cttgtcccaa atttgtttca gttgtcgaaa gtaatgaata ccattcatcg gtggcgaaaa
     8761 aaattgtggc agaaacatta gctcctttaa ccactaaaaa aatcgataca ttgattttgg
     8821 gatgcaccca ttatccatta ttacgcccca tcattcaaaa tgtaatggga gaaaatgttc
     8881 aactgatcga ttctggagca gaaacagtag gtgaagtatc tatgctgtta gattatttca
     8941 atttgagcaa ttcaccgcaa aatggtcgga cattatgcca gttttataca actggctctg
     9001 ccaaactttt cgaggaaata gctgaagact ggcttggaat cggacactta aatgtagaac
     9061 atatcgaatt gggaggaaaa taaggatgcg tcatgacggg agaaaagccc aagagcttcg
     9121 caaactggag cttcaaacaa atgttttcaa atatccagaa ggatcggtag tgatccgttt
     9181 tggtgataca acagttattt gttctgcaac aatagaaaat tctgttcccc cttttttgag
     9241 agaaacagga acaggatggg tgacagcaga atatagtatg ctgcctcgag caacgagtac
     9301 gagaaatcgt agagaaagca gcaaagggaa actttctggt agaacaatgg aaatccagcg
     9361 tcttattggt cgttccttgc gtgcagtagt agacttggaa aaattaggag aacgtagtat
     9421 catcgtagac tgcgatgtga tccaagcgga tggcggtact agaacagcaa gtatcactgg
     9481 ggcatttgtg gcattgaaat tagccattga aaaactttta agagaaaaag aattgtcaga
     9541 agatccaata aaagaacatc ttgctgcagt tagcgtagga atcttaccag atgggacttg
     9601 cgtcacagat ttggattacc aagaagattc cgcagcactt gtagacatga atcttgtaat
     9661 gacagaatct ggaaaattcg tggagattca aggaactgga gaagaagcaa cgtttgatgg
     9721 tgaacagttg aatgaaatgc tcttttttgg aaaaaacgcg attgaagatt tgatcaaaga
     9781 gcaaaaacat gctttgctaa cagagtttgc tcaaaatgat gagcggatag aagaaacaaa
     9841 aacaatcatc attgcgacaa gaaatccagg aaaagcagaa gagtttcgaa atatgttcaa
     9901 agaagcggga tatcatgtaa aaacattact tgactatcct gaattgccag atgtggaaga
     9961 aactggcagc acatttgaag aaaatgcccg attaaaagca gaaacgattg cacaattgct
    10021 tgatcagcca gtcttagcag atgattcagg tttgaaagtg gatgctttag gcggtatgcc
    10081 aggaatctat tctgcacgtt ttgcagggga acaaaaaagt gatgcgggga ataacgccaa
    10141 attattatat gaattgacag atgtgccgga tgaaaaaaga acagcacaat tccattgtac
    10201 gttagtattc gcggcaccca aaaaagacag ccttgtagta gaagcggaat ggcccggtcg
    10261 agtagcccgt ataccatctg gcgaaaatgg ctttgggtat gatcctttat ttatcccaga
    10321 aggtaagaaa caaacagcag cagaattatc gagcgaagaa aaaaacaaga tcagtcatcg
    10381 ggctcaagca atgaaaaaat tgagtgccga gtggaaacaa tggctggaag gagagagata
    10441 gatgcggtat ttagtagtaa gtgataatca tggagacaga gatatcctcg ttgacttagt
    10501 agatcgttat cgttctgaag tggatatctt tttccattgt ggtgattcgg aattagaagc
    10561 ttctgaccca ttatgggata cattccaagt ggttcaaggt aattgtgatt atggaccagg
    10621 attcgaacag aaaaaagtga tccaaactgg tcaggataca gtattcatga ctcacggtca
    10681 tttatcgaat gtccgatttg gtttgacaca gttagcaatc gaagctcgag ctgcaactgc
    10741 cgatatggtg ttttttggtc atacccatca aataggctgt gagatggatc agaaaatcct
    10801 gtttttgaat cctggtagca tttctcagcc tagaggcccg atccaaatcc catcatatgc
    10861 tcttatcgaa agtttagatg accaaatttc tgttcaatac tataaccgtg cccatcaggc
    10921 agttgaagag ttggcctttt ctttttcgag agagcgctga ataaaaaaac tctaattttt
    10981 aataaaaata agctttattt tatgaatttt gcatccggtt tcggtaaaat agtagtgttg
    11041 aatgaaatag aaatggaggc catcagatga ttggacctat agtaagagaa atgctgctgg
    11101 aaaatcagga aacgtttttg attcctgcag aaaatgtggc aaatgttatg tatcatcatc
    11161 cattgtccca tggtttactt gtgttatcga aggtcggtta ttccaaaatt cctgtattag
    11221 gaaaagatga tcactttgta ggactagtaa gtttatcaga tgtggtcaat aaaatgattg
    11281 acttgcaaac aatcagcatg gacccgttag aaggactaac ggttgctgat gtgacagaaa
    11341 cagatgttcc gacaattgat gaaaattggg aactagaaga agtactccat ttgttagtcg
    11401 attcgccttt tttaccggtt gtcacagaag atcggatttt caaaggaatc attacaagaa
    11461 aagagctgct taaagccgta aacttcatgg tgcatgaatt agaacgtcgt aatatattgt
    11521 ctccgaaaat cgaagaatta aaagaacaaa tcgatatcgt gagctaaaga acgagaaact
    11581 aaaagataat caaaaaataa gtagaaaaaa cagcgtacca gttttgactt tatcttgaaa
    11641 aagaggctgg gacaaaagc
//
LOCUS       DADQRX010000083        11491 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.085, whole genome shotgun sequence.
ACCESSION   DADQRX010000083 DADQRX010000000
VERSION     DADQRX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.085"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            258..1796
                     /locus_tag="J8V14_002155"
     CDS             258..1796
                     /locus_tag="J8V14_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBA0922247.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1793..2758
                     /locus_tag="J8V14_002156"
     CDS             1793..2758
                     /locus_tag="J8V14_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922248.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2745..4067
                     /locus_tag="J8V14_002157"
     CDS             2745..4067
                     /locus_tag="J8V14_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA0922249.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4130..4234
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4308..5051
                     /locus_tag="J8V14_002158"
     CDS             4308..5051
                     /locus_tag="J8V14_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922250.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5066..6058
                     /locus_tag="J8V14_002159"
     CDS             5066..6058
                     /locus_tag="J8V14_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0922251.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6070..6807
                     /locus_tag="J8V14_002160"
     CDS             6070..6807
                     /locus_tag="J8V14_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922252.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6797..7519
                     /locus_tag="J8V14_002161"
     CDS             6797..7519
                     /locus_tag="J8V14_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBA0922253.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7603..8226)
                     /locus_tag="J8V14_002162"
     CDS             complement(7603..8226)
                     /locus_tag="J8V14_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBA0922254.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            8561..8995
                     /locus_tag="J8V14_002163"
     CDS             8561..8995
                     /locus_tag="J8V14_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBA0922255.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            9018..9338
                     /locus_tag="J8V14_002164"
     CDS             9018..9338
                     /locus_tag="J8V14_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922256.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            9479..9670
                     /locus_tag="J8V14_002165"
     CDS             9479..9670
                     /locus_tag="J8V14_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922257.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(9741..9998)
                     /locus_tag="J8V14_002166"
     CDS             complement(9741..9998)
                     /locus_tag="J8V14_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBA0922258.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            10156..10419
                     /locus_tag="J8V14_002167"
     CDS             10156..10419
                     /locus_tag="J8V14_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922259.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            10443..10631
                     /locus_tag="J8V14_002168"
     CDS             10443..10631
                     /locus_tag="J8V14_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922260.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(10714..10899)
                     /locus_tag="J8V14_002169"
     CDS             complement(10714..10899)
                     /locus_tag="J8V14_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922261.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            11114..11485
                     /locus_tag="J8V14_002170"
     CDS             11114..11485
                     /locus_tag="J8V14_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBA0922262.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         3868 a         1884 c         2449 g         3290 t
ORIGIN      
        1 aaataaaagt tgtgtgtgta aaaaagatat agctgattcc aagtgacaat ttcgtataga
       61 tttttatggt attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac tttgttgctt
      121 ggattctttt tttgtcagca tatttagagg tatttttttc ttttttttgt tcgtgctgta
      181 aggttaaggc aagacaagtt agttgggaaa aaggaaagaa atgtttatac ttaaagaata
      241 acagaaagga aggtcgcatg aataatttta tacaaacatt aagtgaaaga aaaggcgagt
      301 tgctggaagc tgcatttcaa catctgtcca tttctctcat ttctttgctg attgcagcac
      361 tgattgctat tcctttagcg atttgggcag ccaatcataa aaaaatggca gaagtcttat
      421 tgcagatcac gggtgtgctc caaacgattc cttcacttgc cttattaggt ttacttatcc
      481 catttgtagg gatcggaact gttccagcat tgattgcact tgttatttac gctttgcttc
      541 cgattttcca aaacacgtat attggtctag ctgaaattga cccgtctatt gaagaagcag
      601 cggtagcatt tggtatgtcc agaatgagac gcttgctgaa agtagagctt cccatcgcat
      661 tacctgtcat tatctcaggt atccgaactg ccttagtttt aatcatcggt acggctactt
      721 tggcagcatt gatcggagca ggtggattag ggacatttat tttgttaggt attgaccgaa
      781 atacacctgt tttgacattg attggtgcga tcagttctgc cttgcttgca attatattca
      841 gcggactgat tcgctggttg cagcataaga aacctcgata ttccatcatt actttagcag
      901 cagtggtgtg tttatttggc ggcgtttctt tatatcagtc tgccttgttt aaaaatgaaa
      961 ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa atgtataaag
     1021 aattgattga agacaacaca aaaacagaag ttgaattaaa accgaatttt ggcaagacga
     1081 gttttttgtt caatgcactg gatcaaggtc aaatcgatat ctatcctgag ttcacaggta
     1141 ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa aagtgctgat ttgacaaaag
     1201 aaaaaacata tgacgaagca gaagaattat tgaataagca attcgatatg acattattaa
     1261 aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa gagatctttt gcgaacgaac
     1321 atcaacttaa aaagatttcc gatttggcag ctatctctga tcagatcaaa gcgggattca
     1381 cactggaatt tattgatcgt tcagatggat ataaaggaat ccaagaagca tatggcttga
     1441 atttttcaag tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc aataatggag
     1501 atgtcaatat tgtggatgcc tattccacag atagccagct caaggagtac gatctagtga
     1561 ctctagaaga tgatcgaggg ttattcccag cttatcaagg cgcaccgctg atgaaaaaag
     1621 attttgctaa agaacatcct gaaatcgtta aagcactgaa taaattaagc ggaaaaataa
     1681 cagaggatca aatgattgat atgaattacc aagtcaatgt cgaaaagaaa aagccgacag
     1741 aagtagcacg aatcttcctg aaaaatgaag gattagtaaa ggaggaagat cagtgactaa
     1801 attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca tcgatgacct
     1861 aaatctggca atcaaaaaag gagaaatctt tgttttggtt ggtccttccg gtagtggaaa
     1921 aacaactact ttgaaaatga tcaatggatt atctaaacca tcagcaggag acatctactt
     1981 caaaggaaaa tcgttaaatg aatataatct gcagaaaatg cggtggaata tgggatatgt
     2041 gctgcagcaa atcgctttgt tcccgaccat gacagtcaag caaaatattg aagtcatccc
     2101 tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt gtagatgaac ttcttcaaaa
     2161 agttggctta tctcctgata tttatcgaga tcgtatgcct agagagttgt ctggaggaga
     2221 acagcaacgg atcgggatca tccgagctat tgctgcaagc ccagatgtga ttttgatgga
     2281 cgagccattt agcgcattag atccgatttc aagaaactca ttacaagaat tggttttgtc
     2341 cttacacgaa gaattgggca cgaccatcgt tttcgttacg cacaatatgg aagaagcaat
     2401 caagctaggt gatcgtattg catttatgaa ggatggagaa attatccaat gtgacacacc
     2461 agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg catttctttg atgaaccgaa
     2521 acagacgaaa gagtggcgag tagaagattt agtcgtcaat ggatatttct taaatgaaat
     2581 accagaaaat gctcgtcaac aagtttgctt tgatacgcct atgaaagaag tatattcgct
     2641 tctttcggtg tatccaagca ttgtgatagt agaaaagcaa agaagtattg ggtcgctgac
     2701 aagtaaagaa atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa acatatgatg
     2761 caatcatcat cggttcaggt gtgagcggct taagtgctgc ttatggattg aaagaagcag
     2821 gtaaaacagt actagtggtc gaagaagatc tatggggcgg gacttgtcct aaccgtggat
     2881 gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg taatcgggta aaacaattga
     2941 gtggaaaagg ttttaacgag atcccaactg ccaactggga agaacttcaa aaattcaaac
     3001 gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa gctgaaatcg
     3061 atcatctttc aggaaccgct cgttttttgg atgattcatc tattgaagtc aacgaagaag
     3121 tcttccatgc ggattactta gttttggcaa ccgggcaaag acctacgatc ttaccagtgg
     3181 agggaaagga atacttgaaa actagtgcag actttttatc attgcctgta ctgcctaaag
     3241 aaatcatttt tatcggtggt ggttatattg cttttgagct agctacgatt gccaatgctg
     3301 cagggagcaa agtaaccatt gtccatcata atcaacggcc attaaaagaa tttgaggcgt
     3361 ctttggtaga ggaagccgtc catcaaatgg aagcatctgg cattcagttt gcttttggtg
     3421 ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg tttagtcgga aaagagacgg
     3481 aattagtagc agatatgatt ttttgtgcga ctgggagaca gccgaataca gaatctttag
     3541 cacttgagca agcgaatatc gtctttgata aacacggaat tgcagtcaat gattatctgc
     3601 aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa accccaaaat
     3661 tgacaccagt cgccactttt gaaggaaatt atgtagctaa gcggataacg gatgcaacaa
     3721 gcgaacctat caaatatccg attataccta cgatcgtcta cgccagtcct aaacttgctg
     3781 aagtaggagt tacaaaaagt cacgcctctt cgtcagatca agtggtagag atggatctta
     3841 ccagttggtt cacctatcat cgtgtcaatg aaccagttgc taaggctgaa ttgacctttg
     3901 accagcaaaa ttatttgatt ggtgctgcag tgattagcga gcaagcagac gaattgatcg
     3961 atgatttgac actcgtcatc aatcaaaaat tgactaaaaa agagttggat agctatatca
     4021 tggggtatcc gacattggca agtgatctct cctatttatt gaaataatca gcatgataac
     4081 taaagcgctt gttgcttttt ccttcttgtg ctacacttta aaaaatgaat acctgtttct
     4141 ggggatgtcg tagacgacat gagagtaagt tgtgaactta gacccattga acctgtcagt
     4201 taatactggt gaagggagaa acatagcaga cgcttgaagc gggttactat gtcgtgccca
     4261 tttcaagcgt tttttgttat tcatattaga agaaaggagg taacaaaatg aagaaaaatc
     4321 tttatccagc agtcagtatg gcattcattt taattatttg gcagattgtt gtcatagttt
     4381 ggaaaatacc tgcctttatt ttacccggac ctgctgctgt attttctgca ttagcggcag
     4441 atgcacaagt attactttcc cattctgtca cgacattaaa tgaagcagtg cttggtttgg
     4501 taatagctag cgttctttct tttattactg ccttggcaat ggattattgg aagtttttag
     4561 aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt gccaatcatg gtgttagggc
     4621 cattacttac cctatggttt ggtttcggta tgcttccaaa agtgatcctt gttgtcttga
     4681 taagctattt tccgatcgta gtttcttttt cggatacatt gaggaaagta tcaaaagagc
     4741 aaatcatctt tttgaaaacg atgggggcag atacagggaa gatttatcga atatataaaa
     4801 taccagcggg tttatccggc ttcttttccg gtttaaaagt ggctgctact tattgtgtca
     4861 gcggagcagt cgttggagaa tggctaagcg ctcaagcagg cttagggtat tacatgatac
     4921 gcgtaaaaaa cagctaccag atcgataaag tttttgctgc gatcatttgt gtcattctat
     4981 tgagtttatt gctgaatggc gcttgttcat tattgaaaaa agggtattat cgtatgcttt
     5041 atatttttta agagaagggg atcaaatgaa aaaattatgg cttttattac cgctactgtt
     5101 gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa taaaggattt
     5161 gaaaaaagtg acacttgttt tggattatgt accgaatact aatcatactg gcatctacct
     5221 agcaaaagaa aaaggctatt ataaagaggc tggtttaaat gtccaaatca tcgaaccagg
     5281 agataattcg acaagtattg gtctagtagg tgcggacaaa gcacaatttg gtgtaagtta
     5341 tcaagaagat gtcacttatg ctcacgcaga tggacagaat ataccagtca aagcaattgc
     5401 tacagtgatc aaacataata cttctggatt cgccacccta tcagacagta acatccattc
     5461 accaaaagat tttgaaggaa aaacatatgc aggttggcaa agtccaagtg aagaagctgt
     5521 cttaaaagct gtgatggaaa aagacggcgg ggacttttca aaactgacaa tggtaggaag
     5581 caatggagaa gggccagaaa gcttaggaaa atcctcagat atccaatggt actttgaagg
     5641 ttgggatatg atcaaagcaa aagaagcggg tattgaacta aactatatcc cattgaaaga
     5701 attagatgaa cggttagatt actatactcc ggtaattatt acgaatgatc aactgatcaa
     5761 aagtgatcca gaattggtac agtcttttat ggatgccacg aaaaaaggct atcaggaagc
     5821 tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag aaaatgatcg
     5881 gacgttttta gaagaatcac aagcattctt gtcaaaaaat tatacggatg atcctgagaa
     5941 ttggggattg atggaagaaa aagtttggcg caattataca gcttttatgc aggaaaacgg
     6001 attgatcaag caagatgtac cttcgcaaga actattcact aatcagttta taaaatagga
     6061 gtgatagata tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa cctgaaaata
     6121 ttagaagaca tccatttcac tatccctaaa gataccttta catcgattgt cgcgccaagc
     6181 ggagccggta agtcaacatt gctgaaaact ttaacaggag tccagcctat gacttctgga
     6241 acgatcaaag tagacgatca aaaagtaact ggtctccata cagcatttag ttatatgccg
     6301 caagaagata tgttacttcc ttggctgagc gtctatcaaa acgttacgtt atatcaaaaa
     6361 atcaatcatc taacaatcaa tgaagaacaa gtaagggatt atctggatat ctttgggtta
     6421 aacggctatg aacatttttt gccggaacaa ttatctggcg ggatgaagca gcgaacggcc
     6481 ttgttacgaa caatcatgaa tccttcttct tacttgcttc ttgatgaacc atttggagcg
     6541 ttagatgcta tgaccagagg gcagatgcaa gactggatgt tgaagctgcc taaaaaagca
     6601 aagcggacta ctttacttgt gacgcatgat atcgaagaag caatttattt atcagacagg
     6661 attcttgtgc tttccgcccg tccggcacat gtgattgctg agattcaagt gcctgaaaaa
     6721 cgacggtcac gtgaatggct actgcagcaa tcagagttga agcaaacgat ttatcagtta
     6781 ttggcaggtg aattagatgt taagtgatgc tcattgtcat ttagatggga gtcagagttt
     6841 agcactcctt cagcaggaac acacgatttt gacgatcatc aattgtgatt cgccagaaga
     6901 atggaaggaa aacagacagc ttgcagcaag taagactcaa gccttaagtt acggtatcca
     6961 tccttggaaa gcagatagct atacgtttga acaagttgag ccttttttga aaaaagcaag
     7021 gatcatcggt gaaatagggt tagataatat ttggacgaat gtaccgatga ctacacaaaa
     7081 aaaagtattt gagcgacagc ttgcttttgc agccataaat gagaaacctg ttgtactgca
     7141 cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa gcttacccta atcactattt
     7201 gatccattgg tattcttctt tggactatca acaagactat attgatttag gctgttattt
     7261 cagcattggt cttgatttga aaaagaatcc agcagtctgg caactagcta aagcagtccc
     7321 aatcgaccac ttacttattg aaacggatgg actacatgca gcagagtggg ctttaggggg
     7381 gattttttca gaaaaagatt atgtctcact gcttgaagaa cagttaaaga caatcgctga
     7441 gatcaaagag ctatcagttg aagaagttaa aaaaaatact tatcaaaact tggaacgttt
     7501 cattagatta ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc tctttttggc
     7561 tttctttgta taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc aaccgtttgc
     7621 ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc
     7681 tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta
     7741 acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat
     7801 ccattctttt gcagagcttg tgcttgctgg agtagcagca gtttctgtta cagcaggagc
     7861 aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt cttgtacaac
     7921 aggttgttca gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg
     7981 tgcagagcct tctgttggga ttgttaattg ttgtcctgag taaatcaggt taatatcaga
     8041 aattgaattt gattctgcaa tggcattgat caaggagtta tcaccaacgt atttttgaga
     8101 aatcgttgat agagtatcac ctgattgaac ggtataagct tcgtccgcat gagcagtcgt
     8161 tcccatgaag aatgcagcgc cggcagccaa agttgttcca aaaagtaaag ttttaagtga
     8221 tgtcatgaaa aatctccttt attcgttaaa aagttttatt tgttgtgttc aacgaggaat
     8281 actttaacac gtgaaaatat ttcataggta aaagaagcat gacaatttat gacaaatcca
     8341 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
     8401 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
     8461 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
     8521 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
     8581 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
     8641 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
     8701 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
     8761 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
     8821 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
     8881 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
     8941 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
     9001 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
     9061 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
     9121 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
     9181 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
     9241 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
     9301 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     9361 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     9421 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     9481 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     9541 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     9601 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     9661 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     9721 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     9781 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     9841 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     9901 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     9961 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
    10021 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
    10081 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
    10141 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
    10201 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
    10261 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
    10321 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
    10381 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
    10441 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
    10501 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
    10561 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
    10621 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
    10681 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
    10741 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
    10801 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
    10861 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
    10921 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
    10981 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
    11041 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
    11101 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
    11161 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
    11221 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
    11281 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
    11341 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
    11401 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
    11461 gaaagcatta gcaattattg ggtaaatcag t
//
LOCUS       DADQRX010000084        11445 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.086, whole genome shotgun sequence.
ACCESSION   DADQRX010000084 DADQRX010000000
VERSION     DADQRX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.086"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..53)
                     /locus_tag="J8V14_002171"
     CDS             complement(<1..53)
                     /locus_tag="J8V14_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBA0922263.1"
                     /translation="MLVFQMRNVDKTSTVLK"
     gene            complement(102..341)
                     /locus_tag="J8V14_002172"
     CDS             complement(102..341)
                     /locus_tag="J8V14_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBA0922264.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(475..1692)
                     /locus_tag="J8V14_002173"
                     /pseudo
     CDS             complement(475..1692)
                     /locus_tag="J8V14_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            2183..3121
                     /locus_tag="J8V14_002174"
     CDS             2183..3121
                     /locus_tag="J8V14_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBA0922265.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3251..3898
                     /locus_tag="J8V14_002175"
     CDS             3251..3898
                     /locus_tag="J8V14_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HBA0922266.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4591..5529)
                     /locus_tag="J8V14_002176"
                     /pseudo
     CDS             complement(4591..5529)
                     /locus_tag="J8V14_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5529..6146)
                     /locus_tag="J8V14_002177"
     CDS             complement(5529..6146)
                     /locus_tag="J8V14_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922267.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6678..8168)
                     /locus_tag="J8V14_002178"
     CDS             complement(6678..8168)
                     /locus_tag="J8V14_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HBA0922268.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8211..8510)
                     /locus_tag="J8V14_002179"
     CDS             complement(8211..8510)
                     /locus_tag="J8V14_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922269.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8876..9160)
                     /locus_tag="J8V14_002180"
     CDS             complement(8876..9160)
                     /locus_tag="J8V14_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922270.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9679..10164)
                     /gene="vanZ"
                     /locus_tag="J8V14_002181"
     CDS             complement(9679..10164)
                     /gene="vanZ"
                     /locus_tag="J8V14_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBA0922271.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10317..11228)
                     /locus_tag="J8V14_002182"
     CDS             complement(10317..11228)
                     /locus_tag="J8V14_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBA0922272.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3508 a         2004 c         1742 g         4191 t
ORIGIN      
        1 tgtttcaaaa cagtagatgt tttatctaca ttacgcattt ggaataccaa catgacgaat
       61 ccctccttct taattacaaa tttttagcat ctaatttaac ttcaattcct attatacaaa
      121 attttaagat aatgcactat caacacactc ttaagtttgc ttctaagtct tatttccata
      181 acttctttta cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtca
      241 gcacgaacac gaaccgtctt atctcccatt atatcttttt ttgcactgat tggtgtatca
      301 tttcgttttt ctttttgtgc gcctaaattt cccacaatca ctcacttctt tctatttctt
      361 cttattctta ttttatcatc aacaatcaca aatcacttgt gatttgtgat aagtgatttg
      421 tgataagtga tttgtgatta atatataaaa gccctcttta aagggctttt atgtttattt
      481 tgagaaagat ataaaatcaa tatatccctt ttccccaatt tttacaacgg cattgtaggg
      541 ctttttctct ttgtttttga ttccttttac cagggtttct tttccctcca gtaattcttt
      601 tacattcgtt ttggttagtt ttttctttct aaaatgttca gctaaagtaa acttacattc
      661 aggataattt gaacaaccat aaaacgattt ttttaataca atattgttgc cacacttagg
      721 acattttcct acaatacttt tttctgcttc tttttctttc tgttcctggt aatcagaaaa
      781 atttagtttt tctatatcgt taggtacagc ttccagtaaa tgaacaatga attttttgat
      841 attcgtaata aagttctctt gattgccttc tcttttaccg atttttttta aatacgtttc
      901 ccatttagcc gtcatttcag cactcgttaa aaggtgctga ctttcaactg cctggcacaa
      961 taattttcct ttttcagtta caacaagctt attcttaatc acttggatat attctttttg
     1021 ttttaacgct tcaataatac ttgctcttgt cgcttctgtt ccaatcccct caacttcttg
     1081 taaaatcttg attgcttctt catcatcaac cgttttattc gccgttttca tagcagttaa
     1141 taatgtaccc tctgtaaaag cttttggcgg ttgcgtttct ttttcaacac tcttaacacc
     1201 aacttcggca cgttcgccaa tgataacgag tggtaacgtt tgaacgtcct ctttttcttc
     1261 tttggtttgt tgtttgaaaa gaattttcca cccttcttgc tttggtgtct ttccgatcga
     1321 ttgaaacagc aaatcagcta ctttcgtttg tatcttggtt tcttcataca agtaatcagg
     1381 taaaaacatg gcaacggtcg ttttaacgac taaagcataa atttttcgtt gtaaatcgtc
     1441 cattttcgct aatgcagatt cggtaggtac ttgttttgtt gggataatgg cgtggtgttc
     1501 ctgtacctta ctaccgtcca cataacgctt tctaggctct gtttgaacca tttcaagatc
     1561 aagtcctaaa aaaccgctat atttgccaaa attcgctttt aaataagcaa attcgttctc
     1621 tgtaataaat ggtgtatctg ttcttggata actcaataat tttgcttcat acagtccttg
     1681 catagctttt aaacgtaaaa ttttgcgttc taagacgttt taaatatatc tgcgtatatt
     1741 tttatgtgtt ttatcttttc gtcggcaaat gcgttaaaat ttgcgaaata aaatagctca
     1801 agggcgaacg cagtgagtgc atttaccctt gatctatttt tttgagtaaa ttagcttttg
     1861 aaaccgacgg aaaggaaaca caaaactttt cagaggcaag gggtttttaa ggggcgcttt
     1921 tagccccttg aatttatttg ctttaagata tatttagttt taaaatagtt ttaacgaaaa
     1981 aaagacataa aaaaagacga gcaacgctac caacgttctc gcccggaata acaaaccaca
     2041 cttgttattc attcgatgat tagtatatca atttttgatt ttgaatacaa aacaaaatgg
     2101 acgaatggat acaggcgaaa tttattttgc gattccactc gtcctttttt cgttccaaaa
     2161 attattttag gagcgataaa aaatgaagaa aattgatgaa aaattagctg cagaaatctc
     2221 actaaaaaaa ccggccgtct ctaatagccg gttaagtaat agccggttaa gtggtcaaac
     2281 tttgggaaaa tctcaacccg cattaagttt tgatgccatg acaatcgttg gaaatttgaa
     2341 caaaactaat gctaaaaagc tatctgactt tatgagtgta gagccacaaa tacgactttg
     2401 ggatatactt caaacaaagt ttaaagctaa ggcacttcaa gaaaaagttt atatcgaata
     2461 tgacaaagta aaagcagata cttgggatag acgtaatatg cgtgttgaat ttaatcccaa
     2521 taaactcaca catgaagaaa tgatttggtt aaaacaaaat attatcgact acatggaaga
     2581 tgacggtttt acaagattag acttagcttt tgattttgaa gatgatttga gcgattacta
     2641 tgcaatgact gataaagcag ttaagaaaac tgttttttat ggtcgtaatg gcaagccaga
     2701 aacaaaatat tttggtgtcc gtgatagtga tagatttatt agaatttata ataaaaaaca
     2761 agaacgtaaa gataacgcag atgttgaagt tgtgtctgaa catttatggc gtgtagaagt
     2821 tgaattaaaa agagatatgg ttgattactg gaatgattgt tttaatgatt tacacatctt
     2881 gaaacctgcg tgggctactt tagaaaaaat taatgagcaa gctatggttt atactttgtt
     2941 gcatgaagaa agtatgtggg gaaagctaag taagaatact aagactaaat ttaaaaaatt
     3001 gattagagaa atatctccaa ttgatttaac ggaattaatg aaatcgactt taaaagcgaa
     3061 cgaaaaacaa ttgcaaaagc agattgattt ttggcaacgt gaatttaggt tttggaagta
     3121 aaataagttt tatttgataa aaattgctaa ttcagtataa ttaatattta cgaggtgaca
     3181 taacgtatga aaaaatcaga ggattattcc tcctaaatat aaaaatttaa aatttaggag
     3241 gaagttatat atgactttta atattattga attagaaaat tgggatagaa aagaatattt
     3301 tgaacactat tttaatcagc aaactactta tagcattact aaagaaattg atattacttt
     3361 gtttaaagat atgataaaaa agaaaggata tgaaatttat ccctctttaa tttatgcaat
     3421 tatggaagtt gtaaataaaa ataaagtgtt tagaacagga attaatagtg agaataaatt
     3481 aggttattgg gataagttaa atcctttgta tacagttttt aataagcaaa ctgaaaaatt
     3541 tactaacatt tggactgaat ctgataaaaa cttcatttct ttttataata attataaaaa
     3601 tgacttgctt gaatataaag ataaagaaga aatgtttcct aaaaaaccga tacctgaaaa
     3661 caccataccg atttcaatga ttccttggat tgattttagt tcatttaatt taaatattgg
     3721 taacaatagc agctttttat tgcctattat tacgataggt aaattttata gtgagaataa
     3781 taaaatttat ataccagttg ctctgcaact tcatcattct gtatgtgatg gttaccatgc
     3841 ttcactattt atgaatgaat ttcaagatat aattcatagg gtagatgatt ggatttagtt
     3901 tttagatttt gaaagtgaat ttaattttat acacgtaagt gatcataaaa tttatgaacg
     3961 tataacaacc acattttttg gttgcttgtg gttttgattt tgaatttggt tttgaactta
     4021 tggactgatt tattcagtcc attttttgtg cttgcacaaa aactagcctc gcagagcaca
     4081 cgcattaatg acttatgaaa cgtagtaaat aagtctagtg tgttatactt tacttggaag
     4141 atgcaccgaa taaaaaatat tgaagaacaa ctagcaaaag attttaaaga gttattttat
     4201 tttaagtctt tataacatga gtgaagcgaa tttttaaatt tcgatagaaa tttttacatc
     4261 aaaaagcccc ctgtcaaaat tgacgaaggg ggttttttgg cgcacgcttt tcgttagaaa
     4321 tatacaagat tgaaaatcgt gtataagtgc gccctttgtt ttgaacttag cacgttacat
     4381 caatttttta aaatgatgta taagtgcgcc cttttaaatt ttgagtgatt atattttttg
     4441 agttagaaaa agggattggg aaaatttccc aaaataattt aaaaaataag caaaaatttt
     4501 cgatagagaa tgtgctattt tttgtcaaag gtgtatacct tgactgtgct tgctgttaca
     4561 ttaagtttat ttttaagtta ttaaaaaaga aatagctttt aaagtttggc tcgctgtcgc
     4621 tttataaagc tgattgactt ttgattgcaa actacttaaa gaaaacaaac tcggactatt
     4681 cgttttcttc tctttggttt gaacatcagc aattatcccc tcttgattgc ctattttagc
     4741 ttgtttagaa gaaacaaaag ctaaaagctc ctcttgggtt ttaaaacgct gtgtggggct
     4801 tagaacgccc ttaaacgacc cttggtttac ttttatacta gcttccacct cgaaaaaagg
     4861 ttctttttta aaattctcta tggcttcctg gcgctgaaaa ataaggtata aggtgggcgt
     4921 ttgaacacgt cctagtgaaa atgtaccttg tacgcccttc tgttgtaaat ttaacgtata
     4981 caaagggctt gcgttcatgc cgatcaacca atcggcaatt tggcgtgttt gcgcttcttg
     5041 ataaaaggga tagtaattca ttccaggttg caaattttga aaaccgcttc ggattacatc
     5101 tttttctaag ctattgatcc atagtctttt aaatgtttta tcttttgaaa aggcatttgc
     5161 tttatggata atcgaccagg cgatattttc accttctctg tcgctatctg ttgcaacaat
     5221 aattgtattt gcctttttga gaagttctgc aacaatttta aactgctttc ccttatcttt
     5281 tgcaacttca aaatcgtatc gatcaggaaa aatcggcaaa gattcaagtt tccaattttg
     5341 ccacttttcg tcataatgac ctggttctgc taattccact aaatgcccaa aaccaaaggt
     5401 gataaacgtt tcatctgtaa atagtgggtc tttgatctca aaataaccgt cttttttggt
     5461 gctttgtttt aaagcacttg cgtaggctaa tgcctggctt ggtttttcag ctaaaataac
     5521 cgtactcatt aactatccct cttttcattg ttttttcttt gatcgactgt cacgttatat
     5581 cttgctcgat accttctaaa cgttcggcga ttgattccag tttgttcttc aacttcttta
     5641 tcggataaac cattcaaaaa caaatcgaaa gcatgcttta aacgtggatc attttcttta
     5701 aatttatgct gacgaccttt gaatttgcct tttttcttag caatttcgat tccttgtgcc
     5761 tgacgttcct taattttttt tcgttctgat tctgcttgat acttgtacaa ttcaatgaca
     5821 aggctattaa tcaaacgcct taaattttca tcttcaatac cattcattga gggtaaattt
     5881 aagacttcca gggttgcccc cttaatttga atttgattca tcaattctgt taattcttta
     5941 ttatttcgtc ctaatcgatc taattcagta acaataacaa tatccccttc acgaatatag
     6001 ttaagcatag cttgtaattg tgggcgttcg accgattgac cgcttaattt gtctgaaaag
     6061 accttagaaa cgccctgtaa cgcttgtaat tgccgatcta agttctgttc tttgctactg
     6121 acacgtgcat aaccaatttt agccattttc aaccaacctc taaaattctc tcggttgcaa
     6181 taaccaatca gcaatatcta ctttttcaat ttcaaattgc ttatcagaaa ttgtcttttc
     6241 gtaagcgata aaatcttgcg catattgttg ctcattaaaa atagccacca cttcgtcatt
     6301 ttctaaaact cgataaataa attttttcat tttactcctc ctattatgcc caacttaaat
     6361 gacctattca ccaagtcaat tatactgcta aaatcatatt aggacaaata ggtatactct
     6421 aatgacctat aaaagataga aaattaaaaa atcaagtgtt cgcttcgctc tcactgcccc
     6481 tcgacgtttt agtagccttt ccctcacttc gttcagtcca agccaactaa aagttttcgg
     6541 gctactctct ccttctcccc ctaataatta attaaaatct tactctgtat atttctgcta
     6601 atcattcgct aaacagcaaa gaaaaaacaa acacgtatca tagatataaa tgtaatggca
     6661 tagtgcgggt tttattttca gcctgtatca tagctaaaca aatcgagttg tgtgtccgtt
     6721 ttagggcgtt ctgctagctt gtttaaagtc tcttgaatga atgtatgctc taagtcaaaa
     6781 gaatttgtca gcgcctttat atagctttct ttttcttctt tttttacttt aatgatcgat
     6841 agcaacaatg atttaacact agcaagttga atgccaccat ttcttcctgg tttaatctta
     6901 aagaaaattt cctgattcgc cttcagtacc ttcagcaatt tatctaatgt ccgttcagga
     6961 atgcctagca cttctctaat ctcttttttg gtcgtcacta aataaggctt gtatacatcg
     7021 cttttttcgc taatataagc cattaaatct tctttccatt ctgacaaatg aacacgttga
     7081 cgttcgcttc tttttttctt gaatttaaac cacccttgac ggacaaataa atctttactg
     7141 gttaaatcac ttgataccca agctttgcaa agaatggtaa tgtattccct attagcccct
     7201 tgatagtttt ctgaataggc acttctaaca attttgatta cttctttttc ttctaagggt
     7261 tgatctaatc gattattaaa ctcaaacata ttatattcgc acgtttcgat tgaatagcct
     7321 gaactaaagt aggctaaaga gagggtaaac atgacgttat tacgccctat taaacccttt
     7381 tctcctgaaa atttcgtttc gtgcaataag agattaaacc agggttcatc tacttgtttt
     7441 ttgccttctg taccgcttaa aaccgttaga cttgaacgag taaagccctt attatctgtt
     7501 tgtttgaaag accaatcttg ccattctttg aaagaataac ggtaattagg atcaaaaaat
     7561 tctacattgt ccgttcttgg tatgcgagca ataccaaaat gattacacgt tagatcaact
     7621 ggcaaagact ttccaaaata ttctcggata ttttgcgaaa ttattttggc tgctttgaca
     7681 gatttaaatt ctgattttga agtcacatag actggcgttt ctaaaacaaa atatgcttga
     7741 taacctttat cagatttgat aatcatagta ggcataaaac ctaaatcaat agcggttgtt
     7801 aaaatatcgc ttgctgaaat agtttctttt gccgtgtgaa tatcaaaatc aataaagaag
     7861 gtattgattt gtcttaaatt gttttcagaa tgtcctttcg tgtatgaacg gttttcgtct
     7921 gcatacgttc cataacgata aacgttgggt gtccaatgtg taaatgtatc ttgattttct
     7981 tgaatcgctt cctcggaagt cagaacaaca ccacgaccgc caatcatgct tgattttgag
     8041 cgatacgcaa aaatagcccc tttgctttta cctggcttgg tagtgattga gcgaatttta
     8101 ctatttttaa atttgtactt taacaagccg tcatgaagca cagtttctac aacaaaaggg
     8161 atattcattc agctgttctc ctttcctata aatcctataa aataggttgt ttaattaact
     8221 tggtttgctt tttcattcaa ctgtttcaat attgcatgtt ttgaaaaaga tttttttcct
     8281 ttataagtca atttttttcc actaatcgaa taaattattt tgttattttc tattaactta
     8341 tatatataat cttccccctc cgaagaaaaa tacttatctg attttgtttc taagtagata
     8401 tttctctttt ctaactcttt cttaaacgtt tctagtgtat agatatttgc taattttctt
     8461 atctccaata aactattttt tatataagtt ttacattcat catgattcat acaaactcca
     8521 ccttctataa atgaatacaa aaaaagcaat caaacgattt ccgattgatt gcttaacaat
     8581 tcttaaattc agtagcttag atacttgaaa actctctgat ttccctatat aatgatagta
     8641 cggttatata ccgtcttcaa acaaagttaa ttaaataact tcttacgagg gaagagttca
     8701 tctgactaac tgataagcgt tggtttggca atcttatcgg gctatgcatt tataaaatgt
     8761 cgtcaaacat tttataaatg tgtcatggct cttttttcgt ttctattcag ttcgttgttt
     8821 cgttatatct agtataccgc ttttaaaaaa aataagcaac gatttcgtgc attattcaca
     8881 cgaagtcatt gcttttttct tcttccattt ctaaatccaa tgttacttgt tctgattctg
     8941 tttctggttc tggttctgtt ggctcatttg ggattaaatc cactactagc gttgagttag
     9001 ttaactttgc aatttgttct agtgttttta tggttggatc tgattttcct gattctattc
     9061 gtgaataatt tgatctactc atttctaatt cttgggagaa tttttctttt gatttggtac
     9121 ctctcatctt ttttaagctt tctctaaatg ctagttccaa gtttctcccc cctctttttg
     9181 tgtaataaaa ttcacgcttt atttaaatta tagcttttaa tatcttaata aacaagtgat
     9241 taatcacttg tgatttgtga ttattccaaa agtaggtaat ttcaccaacc tttttcaaaa
     9301 gtttttatcg acgattattg gactggatcg tgtttcctaa agcgattcta agcgcatgtt
     9361 taattttaaa gtgggtaatt ctctttaaaa cgtcctacaa agattcaaaa aaagcatggc
     9421 tgctagtggg ggtaccgtca ggaaaatgcg gatttacaac gttaaggaaa tttattaaag
     9481 agaccaattc ctcagcacta aaattgataa ttgggctttc tttgttacta tagtgttagg
     9541 taagcttttt actttatcac cggttttccc ctcacttcac acctacgggc gagtttcccc
     9601 acatcccgtt ttccatctaa tccaattcat tcaatatctt tatgtgtatg aaagatcaga
     9661 ttgcttgatt tagacatctt acacgtaatt tattcttaaa tgggtacggt aaacgagcaa
     9721 taatacgagc aaaagtatac ctacaaaaat aataactctg tctaattttt tttgattcat
     9781 atgcttattg cttaaaccat ataatttcag tccaagaaag cctccaacag tatttgtaat
     9841 tacatctgtt atgtctgtcg ctccaatagc gaagataaat tgaattattt caaaagtaag
     9901 acttaaaacc agtacaaaag caaacttagg taaaaatccg atttctttaa aattgacatt
     9961 caaaagcaag ccaaatggaa taaagattat aacattatct atcatctctc tgaaattccc
    10021 agtagcagta aatggagtca agttaagact tctttgatga taattaaata ctgataaaat
    10081 attgtattgt aatttgaata acactaacca gattagtgtc actaaatata aagctagcaa
    10141 tcctctagat aatatttttc ccaatataat ttcctctaat tcttttgttt aagttgtatc
    10201 attagtatag caatgtccta gttaacgtgg gttttaaatt aaaaagctaa cgttacccca
    10261 aaagtgctag tacaataatg ctgttcatta atttcgttgg tttcattccg ccatccttac
    10321 ctccttgaat tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg
    10381 tttcctgata tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca
    10441 ggataataaa agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct
    10501 tttaggtaat ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt
    10561 ggtaaaccaa catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg
    10621 tcctctggat aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct
    10681 tcaggggctc gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta
    10741 tgctcactat aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt
    10801 tgctcatcaa agtctcgata gccactatta ataataaaat gactaacgcc accctttaca
    10861 gcatcattga ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta
    10921 tcaagcaacc cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac
    10981 ttcacacttt cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa
    11041 acctgttctt ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa
    11101 tgttctttgg gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct
    11161 tcattaacgt agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac
    11221 ttcttcattt tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat
    11281 cttcccctta aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag
    11341 tcaattattt cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt
    11401 gatacccatg ctgaatttaa cgaggctgaa ctgcctgttg acaat
//
LOCUS       DADQRX010000085        11331 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.087, whole genome shotgun sequence.
ACCESSION   DADQRX010000085 DADQRX010000000
VERSION     DADQRX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.087"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(358..1476)
                     /locus_tag="J8V14_002183"
     CDS             complement(358..1476)
                     /locus_tag="J8V14_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922273.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1947..3254
                     /locus_tag="J8V14_002184"
     CDS             1947..3254
                     /locus_tag="J8V14_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBA0922274.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3373..4572
                     /locus_tag="J8V14_002185"
     CDS             3373..4572
                     /locus_tag="J8V14_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBA0922275.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4599..5867)
                     /locus_tag="J8V14_002186"
     CDS             complement(4599..5867)
                     /locus_tag="J8V14_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBA0922276.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6030..6676)
                     /locus_tag="J8V14_002187"
                     /pseudo
     CDS             complement(6030..6676)
                     /locus_tag="J8V14_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7084..7587)
                     /locus_tag="J8V14_002188"
     CDS             complement(7084..7587)
                     /locus_tag="J8V14_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBA0922277.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7710..8474)
                     /locus_tag="J8V14_002189"
     CDS             complement(7710..8474)
                     /locus_tag="J8V14_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBA0922278.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8582..8857
                     /locus_tag="J8V14_002190"
     CDS             8582..8857
                     /locus_tag="J8V14_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBA0922279.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9295..10245
                     /gene="yidC"
                     /locus_tag="J8V14_002191"
     CDS             9295..10245
                     /gene="yidC"
                     /locus_tag="J8V14_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBA0922280.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10424..10624)
                     /locus_tag="J8V14_002192"
     CDS             complement(10424..10624)
                     /locus_tag="J8V14_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922281.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3398 a         2386 c         2001 g         3546 t
ORIGIN      
        1 gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt tgagaaataa
       61 tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac ctcttcctta
      121 aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa atatgaagag
      181 ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca acctctcata
      241 cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa tatgtgaaac
      301 tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca acctctttta
      361 ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat ccaaaataaa
      421 cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt gacgccgatc
      481 atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag cgataaataa
      541 atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat cgatgaattt
      601 cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac ttgacagctt
      661 cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag aaatcgcaac
      721 gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat tttctggtaa
      781 tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca agtacgtatc
      841 aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg gatttacgcc
      901 atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt tcaaaccgcc
      961 acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt ctggtagaga
     1021 tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc ggttcaatcc
     1081 caataaataa taagaatagt tgaactgccc atttttgata atagccagta aataaggaag
     1141 atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat aggtctggtc
     1201 agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag gatctcctaa
     1261 caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt ttttctcaaa
     1321 acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt ccacatcttg
     1381 atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc cttctgaagt
     1441 caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc taaaaaaatc
     1501 attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa taaaacgtat
     1561 ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa gttatcttca
     1621 taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg ctgataaact
     1681 aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca gtttattaag
     1741 tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa cacagcctat
     1801 tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc ttatcctaaa
     1861 gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat ttgtagcgaa
     1921 acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta gtcctattat
     1981 tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg caagctgaag
     2041 atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc ggtaagattc
     2101 tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa atcatcggtc
     2161 tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat aaagtttcga
     2221 tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt cctttgcata
     2281 aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag tcagccaatg
     2341 cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc actgaacgaa
     2401 tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat gcatcaggat
     2461 tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac gacgaaaatc
     2521 aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat ttcccagaaa
     2581 tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa tcaccagtgg
     2641 aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa ggcgtggacg
     2701 gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg atcactaaaa
     2761 acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa aacccgagtg
     2821 cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg tcagaaaaag
     2881 agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta aaagacggaa
     2941 aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta cgaaacgata
     3001 tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc actgacaatg
     3061 agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc accttggcag
     3121 acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc attacggata
     3181 aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca gaatttttcg
     3241 gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga tgtttttttg
     3301 ctttttctag tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa
     3361 ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta tcttattggg
     3421 gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt cttttacggc
     3481 agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat tttttattcg
     3541 accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc ttatttcaca
     3601 gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag tttcggcatc
     3661 tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact gatcgccttg
     3721 atcacgattc cattgctact tgtcctttta tttggtattt atggattgat tgtttttctt
     3781 ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa tcttttaacg
     3841 ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt gttaaaatat
     3901 ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat ctatctattt
     3961 atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg gcctcgatac
     4021 aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac agtttctcct
     4081 cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag ccgtgctaca
     4141 ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa agtaccagag
     4201 gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga cattcttgtc
     4261 gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat catggatcag
     4321 caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat ttttagagcc
     4381 ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa aacagctttc
     4441 tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat gaacaaaaaa
     4501 agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc gctgattact
     4561 ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttc
     4621 attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg ctgagcagaa
     4681 taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa gaagtatact
     4741 gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc
     4801 gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga aacatggagc
     4861 aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt cacttctccc
     4921 ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat aaaatcatag
     4981 acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc gagataacgt
     5041 tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt atttcgacaa
     5101 acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga acggctgaat
     5161 acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata
     5221 ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc
     5281 atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt tcggactttc
     5341 ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa
     5401 ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt atccaatcgg
     5461 aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt tccttcacgg
     5521 ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag tggaataagc
     5581 cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt gtttccacga
     5641 ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact aactggtagt
     5701 agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag gctatttaag
     5761 aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac cagccacttc
     5821 agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa aaattcctcc
     5881 tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta ggcgtcatta
     5941 gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc attaaaagat
     6001 agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg ttcagcaaat
     6061 tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc atgacaaata
     6121 taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc
     6181 gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt tggtgataaa
     6241 gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt tttctgcgta
     6301 cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt
     6361 tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct aggctgcata
     6421 aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt
     6481 tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga tctaatatgt
     6541 ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga tcattgacag
     6601 atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat tggtatggtt
     6661 ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat actttgtaga
     6721 aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt ttaaataaga
     6781 tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta catgattcat
     6841 tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca gaaaaaagga
     6901 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca aaagctggca
     6961 tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc
     7021 ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta ttggttttat
     7081 ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca tacttgctga
     7141 cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta cgatatatcc
     7201 ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa tgatatctcg
     7261 ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc ttggatggat
     7321 atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga acagatcatg
     7381 caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg cgagcagata
     7441 actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat gctgtgtgta
     7501 attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag acatatattc
     7561 ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt gaatttcatt
     7621 ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt cgccttggat
     7681 aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag aacaatccct
     7741 gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata tacgttctga
     7801 tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat gattattgcg
     7861 aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa ttctgtgcca
     7921 tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc tttttgcaac
     7981 acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact ataaatatct
     8041 gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt acggatcatc
     8101 gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc tgaaaaatct
     8161 ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt aggtagatcc
     8221 gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt gtttgcggcc
     8281 agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt tatttctgca
     8341 tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc ttccttttct
     8401 tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt ttttgctgat
     8461 tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg tgcttgagct
     8521 tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa aggtgacgaa
     8581 catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat ttcgctacat
     8641 gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg aagatgacgg
     8701 ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta ttgaagaagt
     8761 caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag aagacccgat
     8821 gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag ctggtctgca
     8881 tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca ggctggactt
     8941 aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag gcgctttgcc
     9001 tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag ttcatttgac
     9061 tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag aaacacttgg
     9121 aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt tcaaaatttt
     9181 tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg
     9241 cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat
     9301 aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc
     9361 tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta tcgagtactc
     9421 gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg gtcttatggc
     9481 tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact aggtattagt
     9541 caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa
     9601 gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc agcacaagca
     9661 gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat cggctgtctg
     9721 cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg ttatacagaa
     9781 ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat ggtacttgtt
     9841 gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg tattcctgaa
     9901 gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg
     9961 tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt
    10021 ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa
    10081 gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt gacagaatca
    10141 gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa aaacagtaca
    10201 aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt aaataagagt
    10261 gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat ttttgaaaaa
    10321 gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg attgatcgct
    10381 atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta tcaacgtaga
    10441 caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt tttccttctt
    10501 ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc actcccttcg
    10561 cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg gagataccac
    10621 ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta tccatccctt
    10681 tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt cccttcttat
    10741 aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa ctggctcctt
    10801 tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact acaaatttct
    10861 tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag gatggtattt
    10921 aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc tttaaagaca
    10981 ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat gaacttatct
    11041 tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc ataccggaca
    11101 actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa atgacaatga
    11161 aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg cgactgctct
    11221 tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta gttcctcatc
    11281 tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat a
//
LOCUS       DADQRX010000086        11193 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.088, whole genome shotgun sequence.
ACCESSION   DADQRX010000086 DADQRX010000000
VERSION     DADQRX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.088"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..902)
                     /locus_tag="J8V14_002193"
     CDS             complement(9..902)
                     /locus_tag="J8V14_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922282.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="J8V14_002194"
     CDS             complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="J8V14_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBA0922283.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1677..2778)
                     /gene="prfB"
                     /locus_tag="J8V14_002195"
     CDS             complement(join(1677..2714,2716..2778))
                     /gene="prfB"
                     /locus_tag="J8V14_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBA0922284.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2904..5438)
                     /gene="secA"
                     /locus_tag="J8V14_002196"
     CDS             complement(2904..5438)
                     /gene="secA"
                     /locus_tag="J8V14_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBA0922285.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="J8V14_002197"
     CDS             complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="J8V14_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBA0922286.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6339..7058)
                     /locus_tag="J8V14_002198"
     CDS             complement(6339..7058)
                     /locus_tag="J8V14_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBA0922287.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7055..8368)
                     /locus_tag="J8V14_002199"
     CDS             complement(7055..8368)
                     /locus_tag="J8V14_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBA0922288.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8449..8769)
                     /locus_tag="J8V14_002200"
     CDS             complement(8449..8769)
                     /locus_tag="J8V14_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922289.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8877..9164)
                     /locus_tag="J8V14_002201"
     CDS             complement(8877..9164)
                     /locus_tag="J8V14_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922290.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9291..9935
                     /locus_tag="J8V14_002202"
     CDS             9291..9935
                     /locus_tag="J8V14_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBA0922291.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9994..10701)
                     /locus_tag="J8V14_002203"
     CDS             complement(9994..10701)
                     /locus_tag="J8V14_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922292.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         3051 a         2430 c         1956 g         3756 t
ORIGIN      
        1 ttgtggtatt agaatttcaa aaaacgtctc atagaaatga tcgagccaag tgaaccaatg
       61 atcacaccaa tgccgaccat taaaagattg attttccaaa cgatattttg tggacgaatc
      121 aatgaataat gggaacgtaa taatgatgga ttgatcaatt ggaaaaacca tgcataacca
      181 aatgtgatga tcaaaatcgg aagaatcgca ccgatcagac cgatccagcc accttcaagg
      241 aaaaacggcc agcgaatata tccatttttt gcaccgacta aacgcatgat ttgaatttca
      301 cgctgtctag acaaaatcgt gatccgaatc gtgttcgaaa tgagaaacat cgctacgaac
      361 agcagcaatg ccgctgcaac taatccccat gtccgaactg caccagcgat ttggaaaatc
      421 cgatctgagg aagctccacc gtaatcagaa cgagaaacat tcttcaaatc tgctgcttct
      481 tctgctactt gttttgtata agaaggatca gtagcactta ctacatacac atcatataac
      541 gggttattgt caccttcaaa caatttccag gaatcaccca tttcgttttg gatttttttc
      601 aattcctgtt ctttgcttga aaattcaact tttttaacat gatctaagtc attcaattct
      661 ttttctagct ttttcatctc ttcgtctttt gtaccgatat ctacgaatac agatacatca
      721 acgtttcctt ctatatcctg agctaattta gttgcattca tgataactgc catgaataca
      781 ccgactaaag tcaacgtgat cgttaccgca ctgactgaag caactgtcat ccagccgtta
      841 cgtttcaaac tcttgatact ttctaataaa tggcggaaaa aggttctaat catcgtatcc
      901 atattcccct tccgcttgat ctcgaataat gcggccgttt tcgatagcaa ttactcggtg
      961 ccgaatcgta tttacgatcg tactgttatg tgtagccatc acgacagtag ttccttgggc
     1021 attgatccga tctaataatt tcatgatttc ccatgagttt tctggatcca gatttcctgt
     1081 cggttcatcc gcgataagta ctttaggcgt attcacgatt gcccgagcaa tagagacacg
     1141 ttgctgttcc ccaccggata attcgctagg aaagactcga actttgtgtt tcaaaccaac
     1201 aaggtccagc acttccatta ctcgtttttt gatatctcga ggttttctgc cgataacttg
     1261 catagcgtac gcaacatttt catatactgt ttttcttggt aataatttat agtcttggaa
     1321 caccacgccg atttctctgc gtagataagg aacttcgcgg tttttgattg ctattagatc
     1381 atgacctgcg acgttcaaag ttccttttga agctttttct tctcgataca tcaatttgat
     1441 gaatgtagat ttaccggctc cagacggacc aaccacatag acaaactctc cttgttcgat
     1501 gacaatcgag agattgcgga tagcggtcgt accatttgag tacttcttca ttacatcctt
     1561 catttcaatc atggcgttct ctccatatct ttttcagtct ttagctatta tagcatggca
     1621 atgtttcaac atgcttgcaa aatcttacaa ttttatttca atttaacaat gagttcttat
     1681 tgtagatgca tttttaaata gccgtcaata aataaatcaa ggtcaccgtc cataactgct
     1741 tggacatttc ctgtctctac gtttgtccga tggtctttta ccatagaata gggatggaaa
     1801 acataagaac ggatctgtga accccagccg atttcttttt gttctcctcg aagtgcagct
     1861 gcttcttgtt ctttcttttc cacttctaat tgataaagct tagcttttaa catccccatc
     1921 gcttgctcac ggtttttcaa ttgtgagcgt tgtgcctgac tagctactac tgttcctgta
     1981 ggcaagtgag tgatccgtac agcagattct gttttattga tgtgctgacc acctgcacca
     2041 cttgctcggt acgtatcgat tttcaaatca tctggattga tttctatatc gatcgtttca
     2101 tcaagctctg gcatcacatc taccgaacaa aaagaagtat gacgtcgttt cgctgagtca
     2161 aaaggcgaaa tccgtacaag gcgatgaaca cctttttctg attttagata accaaaagcg
     2221 ttatgtcctt taatcagcag ggtcacactc ttgatccctg cttcatcacc tgcttgataa
     2281 tctagtgttt ctacttgata gccatgctgc tctgcccaac gtgtatacat acgtaaaagc
     2341 atgctgcccc agtcttggga ctccgttccg ccagcccctg gatgcaattc gacgatcgca
     2401 ttgtttcgat cataaggtcc gtctaacagc atagataatt catacgtctt cattttttca
     2461 tctaatgcat tgatacgtgt ttctgcttct tcttgcattg ctgcatcagg ttcttcttgc
     2521 agcatctcca ccatcaattc gatttcttct agttcttcgg caagagaatg gaattggtca
     2581 taggtttctt tattagcgtt attttcgctg atcacttttt gtgcggcttc gctgtcatcc
     2641 caaaaaccag gttcagccat ttttgcttct gcttccgcga tgtcttcttc cagctgatct
     2701 aagtcaaaga gacctcctga aacttgtgac cttttcctgc atttctgtca ggagatttcg
     2761 aatttcggat atttccatgt tttttccttc tttcactata cttttttcat tatatctagt
     2821 ctaacagaat cagcaaataa cagaaagaag gtgagacgaa ttcttaaaaa aagaatttgt
     2881 ctcaccaatc ctgctgtcag aaattagttt gcattttttc catgacagtt cttatatttt
     2941 ttcccgcttc cacaaggaca aggatcattt cttccgacct tgttcacatg gattggctgt
     3001 tttttcgcgc tagtattact attttctttg gcatctccgt cttctgagtg agaagctgtt
     3061 ccttgagaaa cctgttcacg ctgtacgttt tgacggatct cagctttcat aaagattcga
     3121 gtgacttcat actcgatggc gcctaccata tcttcaaaca tcttatagcc ttctgtttga
     3181 tattcgacta atggattgtt ctgtccataa gcacgtaagc cgattgactg acgtaattga
     3241 tccattgcat caatatggtc tgtccattta gagtccacaa cacgcaagat cacgactttt
     3301 tcaaattcca acagttgctc tggaccattt agttgagccg ctttggtatt aaatacttct
     3361 tgtgctcgtt cgtttagata ctctttgatc tcctgagatg tttttccttc gatatctgca
     3421 agagtaatac tatcttcatg gacaagagta ctgccggcaa aatcgatgat actttccaaa
     3481 ttccaatttt ctttctctaa ttgtgtatgc gcatctacta cacgactgat tgtccgtttg
     3541 accatgttca ttagtggctt ggataagctt ttttcttcca tgattacttc ttgtcgctgt
     3601 ttatagatta cttcacgttg ttcacgcatc acatcatcgt attgtaagac gtttttacgt
     3661 gtgtcatagt tatttccttc tacacgtttt tgtgcagatt ctacttgttt cgtcagcatc
     3721 ttgctttgga taacagcatc ttcctcaccg atcttcatcc gatctaagaa agctttgatt
     3781 cgttctgaac cgaatcgttt catcaaatca tcttccaatg acaagtagaa ttgtgacata
     3841 ccagggtctc cttgacgtcc tgcacgtcct cgaagctggt tatcgatacg acgagattca
     3901 tgtcgttctg tcccgataac tgctagacca ccaagttctc ttaccccaag tcctaacttg
     3961 atatctgttc cccgaccagc catgttggta gcaatcgtca cagcaccttt ttgaccagca
     4021 ttcatgatga tctctgcttc tttaaagtga tttttcgcat tcagcacttc atgaggaata
     4081 cgttcttgat tcagcatatc tgataataac tcagatgttt caaccgcaac agttccgact
     4141 aacacaggtt gacctttgcg gtagcgatct ttgatatctt gcacgacagc gtggaacttg
     4201 ctttgtaatg ttgggtaaag taaatctgga cgatcatcac ggataaccgg acgatttgtc
     4261 ggaatctggt atacttggat gttataaatt tctctgaatt cttcttcttc tgttttagct
     4321 gtaccagtca ttcccgcaag tttcttgtac atacggaaat agttctggaa agtgatcgtc
     4381 gccatggttt tcgtttcgtc ttcgatctct acgccttctt tagcttcgat tgcttggtga
     4441 agtccatctg aataacggcg tccatccatg atccgaccag tgaactggtc aacaatcagt
     4501 acttttccgt cttggacaac ataatcgata tctaaaagca tgatatagtt ggctctcaat
     4561 gcttgatcca agtgatgggt caatgcagta ttttcaatgt cgtataagtt atccaaaccg
     4621 aatgtttctt ctgctttttc gatccctgct tcggttaaac tgattgtttt tgattgtaca
     4681 tcgattttat aatcttcttc ttctttcaaa cgtttcacga agttgtctgc tcttgtatat
     4741 aaagctgtgg atttttctgc ttgccctgaa atgatcagcg gcgttctcgc ttcatcaatc
     4801 aagattgagt caacttcatc gacaatggca taattcagcg gacgttgtac catttgatgg
     4861 cggtaaacga ccatattgtc tctcaagtag tcaaatccta attcattatt cgtactataa
     4921 gtgatatcac aattataagc ctcgcgtttt tcatccgaag attttgagtt gatattcaac
     4981 cccactgtta atcctaagaa attataaagt tcgcccattt ccgtcgagtc acgggttgct
     5041 aaatattcat tgacggtaac tacgtgtacc ccttcgccag tcagagcatt tagatatact
     5101 ggcatcgttg cggtcaaggt tttaccttca ccagtacgca tttcagggat atttccatcg
     5161 tgaagcacga ttccacccat caattgtacg tggtatggat ataaccccaa tacacgtttt
     5221 gctgcttctc ggacgactgc aaaagcttcc ggaagtaatt gatctaatgt ttctcctttt
     5281 tggtatctgc ccttgaattc agcagttttt gcctgtaatt gttcatcagt taatgctgcc
     5341 atttggtcag cgtatgcttc aacttttttt gcaatgctat ctaaacgttt tagttctttc
     5401 ttatcgtttt cgataagtga acgaatcaaa ttggccatgc gttgtgtaat ctccttttcc
     5461 ttctgtgtat tatccagact atgtagactt tacttttcat tttatcatta gttatagata
     5521 attgaaatat cttaagcgta ttaaagtaag aacttcataa tttattcata tttcatctaa
     5581 aagaggagga aactcaaaaa aacttgcggc agctgtcagt agctgctgca agttttcgtt
     5641 gttatttaag taaggctgat cagtctgttt caatcagtcc gtattttcca tctttacggc
     5701 ggtaaacgat acttgtacca tttgtttctg catcttcaaa aataaagaag ttatgtccca
     5761 gcatgttcat ttgtaagact gcttcttcac tatccattgg tttcaatgaa aggcgtttcg
     5821 tacgtacgat atctaattca gaatcatttt cgttttcgtc ctcttcattg aataaagcag
     5881 cagcatctgc agtattcatg cccgtttcac gggatttacg attaattttt gttttgaatt
     5941 tgcggatttg gcgttccaat ttatcaacaa ccaaatcgat acttgcgtat aaatctggag
     6001 aagtttcttc ggcacgtaat acaagataag gtaatgggat cgttacttcc acttttgctg
     6061 ttttttctgt ataaactttt aagttcacat gcgccgttgc atcgggtgcg tcactgaaat
     6121 agcgttctag tttgcctact tttttctcaa cgtagtcgcg aatcgcttca gttacttcga
     6181 tattttcgcc gcgtacatta tatctaaaca taaaaattac ccctttcgtc taccacaatc
     6241 aagaaggaga aggaccactc tttccctctt cttaattcta ttatatcgaa ctctaggtta
     6301 tttgcaaatt aaatcgctat caaatttcca catctaattc atctcgccaa agaaatggtt
     6361 cggatgattt tcggctgtgc gacctgcaaa caatctgctg cgtggaaaag agtctgtcct
     6421 gttgtataga catcatcaat aatcaatatt tttttatttt gtatcttaga aaaggtgact
     6481 gcaagttcaa aaggttgagg catctccaat ctttcctttc gcgtgttttt tgcttgagcc
     6541 agcagatgct tctctctttt gagtagcatc tgaaaaggaa ttcttgctgc tttcaacatc
     6601 tcgcttactt gattgaatcc tctttctgtt cgcctttttt cagataaagg tattggacaa
     6661 tagatgtatg tcgggtattc tttttttatc tgcgtccatt tatatgcaaa ggtgcctgct
     6721 agtcggatat ctcccataaa cttgtattga ttgagccagg ccttcatatt ttcatcgtac
     6781 acaaaaaagg cacggtgctg aaaatcatag gtcggatatt tcttcttcca ttcttgacag
     6841 tcactgcagt aaacgggaat agactttact ttctttttcg tactctctag attgaaatgg
     6901 attggttttt gacagccaga acagcctctt gtttccaata aatgaaattg tgcgcgacaa
     6961 actgaacaag tctgaggtaa tctgtatttc gaaacaccaa taatctcgct gaatgttaaa
     7021 ttttggaaga tatctttact gcagcaactg cacctcacag catcttcttc ccttcttcat
     7081 tcatttcttt gatttcctga caagcttttc gaatagctgc agttttttct tcatagaaaa
     7141 agtatacttg actgttcgta taaggccctt tacgatcggc tcttcctgcg atttgtacca
     7201 aagaagattt agtaaaaacc ggatgatccg ctcctaaaac gataacggat attctttcaa
     7261 atgtgactcc acgttcaagg atcatcgtgg ttaacagtat atcatatttt gcttctcgca
     7321 ttttctggac tttttccgcc cgctgattgt ctcttgaaga aacatctgtg atctcatatc
     7381 tcggaagagc ctcttgaagt gtggctttca aacgatgcat catcgtgata cttggacaaa
     7441 ataaaaggat atggttttct ttaattaatt gatgaatgat ccgcaccaaa gattttatct
     7501 tcttcttgtt cttgcatttt tgtcgccaat tattcatgaa aaccaagacg ggttcaggaa
     7561 gcaaacgccg atgaaagcgt aaagcaagtt tgtggatgtc aaacgtctgc ttgatttcct
     7621 ttagtaactt tttatcaggt gtggcactta agtagatgaa tcgtccgtct ggtttaatcg
     7681 ctgtttgcac agcatgagct aatcctgcat ccccgttata aggaaacgca tcgatctcat
     7741 cgatcactat taaatcaaag gaccgataaa aacggtatag ctgatgaatc gtacagataa
     7801 caaaagaagt aaaggtataa gccgttgctg tattcccatg gagcaacgtg attttttctt
     7861 ctggaaaaac ataacgatag cgtaaaaaca attcattaca aacatcgact cgcggtgtac
     7921 aaatcgctac tcttcctcca gcttttaaag tttccactaa aataggataa agcatctctg
     7981 ttttccctgc acctgttaca gcccaaataa gatgatgtct tttttctgaa tggcaaagta
     8041 ttctctttga gatttccgtt tgtttttcag taagctgtcc tgtccaaaaa aagacgactt
     8101 ctcgaggttt tgcttcaggc tgatcaaaca aatataaatc ttgttgagtg tcgcatcgcc
     8161 ctaaatatag acaatgtgta caaaaataac cgtaatctgc aagctgtcca tcaatgacgg
     8221 acgtcctcat tccacagcga tgacaaagaa tccagttgcc caaacgttcc actgctgcga
     8281 tcttctcata atttggtaaa tttacctcct cacagtctat tgcttcaata tctctcgtca
     8341 gaagccttct accccaaaaa tgattcatat tcttcgccct aaaaaaatat acgaaaaaac
     8401 aagcgatcta ttttttggat cgcttgttcg atagttatac tgttctattt attcacctat
     8461 gagcgtgatt gcttgatcta acactttttc gccattcatc ataccataat cagccatatt
     8521 gatgacatct aaaggaatgc cttttggttc cagacgtttc tcaaaatcag ctttcataaa
     8581 gcgaacttgt gggcctaata acaacacatc cacgtcttta tttgcaaggt tgttatctgc
     8641 gtcagaagcg gacacagcga aaatatccga gtcgattcct tttgcttcag cagctttttg
     8701 catttttgtt actagtaaac ttgtgctcat accagccgaa catacaagca tgattgtttt
     8761 tttagccata cttttctctc ctttttaaaa caaactatgc aaacgttttc ttcgcgtctg
     8821 ttactctatt atataaaaag aaagaaattt gtcaacgttt tctaaacgcg gtttatttaa
     8881 tcaaatagcc aactagattt cactaattta ttgaacacga catattcttt gtccaaattg
     8941 atgacaacta cttcatagcc tgcatttatc cagccatagg cacctttttc agggtgttta
     9001 taatcatttg cccaccaatc tgtactttcc cttgctgttt tgggaagtcc gttactggga
     9061 aacagcagtt cgtcaaattg ggtaaaggtc aacgttactt gagagccgcc attgttcttc
     9121 aaataatgcg taattgcatc gtactttttc attcgttcct gcatccccag atccacctgc
     9181 ctttgtcttt attatcgcat actacagcaa ttgcgcaacc gttacagtgt tgaaacaacc
     9241 tagaacttta tttatactga ataaagaaaa tgaaaaaaag aggtgacaag atgatcgagc
     9301 gctatcgtac aataaaagaa aatggacaaa gtgagattga gatcaaaaaa tcacgcttta
     9361 tctgttctct tagacgaata gaatcagagg accaagcaaa aagtttcatt caagaaataa
     9421 aaaaagaaca ttggaaagcg aaccataact gcagcgcctt catcattgga gaaaaaaatg
     9481 aaatccagcg aagcagcgat gatggagaac caagtggaac ggcgggcgta ccgatgttgg
     9541 aagtcctcaa aaaaaatcaa ctgatcaatg tagctgcagt agtcactcga tattttggag
     9601 gaacgaaact cggcacaggc ggattaatac gagcatacac tcatgcggtc tctcaagcat
     9661 tgtctgacat tggaatcgta gaaggtacct tgcaacagga gatctctgtg atgatcacgt
     9721 atccgcaact aggaaaatta cagaattatc ttgaactcca acattttttt ataaaagaaa
     9781 tccaatatac tgatcaagtt agcgtcatct gtgctgtaga tgaaaaaagt gctgaccact
     9841 ttaaagaatc catcattgag ctgctgaacg gacaagtcca atttgaagag ggttctgttt
     9901 cttatcacga gcaactgata actgatcata tataaaaaca ggtgattctg attctctttt
     9961 atttctaata aggaatcagg gatcacctgt tttttatttt tccaaataaa actcaaaacg
    10021 atttcctacg tattgcgtac gcacatactc aaacggcgta ccgtcttcaa aataagaaat
    10081 ctggcgcaaa cgaagaactg catctcctcg ttttacctct aaatagtcag caattttctc
    10141 agaagccaac acagcagtaa tcgtttgatt cgctggaccg atctttttcc cgcctttttc
    10201 ttctagcgtg cgatacaacg attcagtaat ctctgctttg ctgtagccat cgatcagctt
    10261 ttgtggaaca ctggcaactt caaaacaaat agggacatca tctgcgaaac gaatacgttc
    10321 cattcgcaag atcgtgtcat ctttactcaa gtttagtttt tccatttcac ttgagcttgg
    10381 cgaagtcaag aaataagaga ttgtccgact agacggtact cgattttgtg attccataat
    10441 ttctgtaaaa cttgttgcac cagacatttt ttcttgtact ttttctcgcg caacatacgt
    10501 acccgaaccg atctttcttt ctaagattcc ttcatctgcc aagttttgaa ttgcttgtct
    10561 caacgtcata cggctgacat tgaatttcac tgctaattct ctttctgaag gaagacggtc
    10621 tccgactttc catataccct gttcgatttc attttttaat tggtcatgaa tctgtatata
    10681 tacaggtaat tgattcgaca taacgtacac ccgtcctttc gtttcttcta ttataactgg
    10741 tatatacctg attcacaact gaaaaagata aaggtttcta tccatcaaca gaggatttag
    10801 acacacatcg ttctccctac tcctattgtc agaaacaatt ttttagatat gttcaaccta
    10861 gaaaataaca aaagacccta aaatcgatga aaatgccgac ttatggatct tttgttatta
    10921 ttagcggttt ttagccatcc atttttttgt atgattcgtt gggcgttttg attttacctg
    10981 gaccgctttt ccgccttttt tcggaatatt cacttcaata ttgtattttt ctgatttgac
    11041 caaatcatat aacttttcag ctgcttcgtc tactttttct ttccatttca tgtggatccc
    11101 tccaattctt gtagttaact taactctatc ttaattcagc agaatcttca aggaacaagg
    11161 tagcaaagca aaaaaaagag ctctcgaact aac
//
LOCUS       DADQRX010000087        11128 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.089, whole genome shotgun sequence.
ACCESSION   DADQRX010000087 DADQRX010000000
VERSION     DADQRX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.089"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="J8V14_002204"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="J8V14_002204"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBA0922293.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="J8V14_002205"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="J8V14_002205"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBA0922294.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="J8V14_002206"
     CDS             2139..3020
                     /locus_tag="J8V14_002206"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBA0922295.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="J8V14_002207"
     CDS             3017..3733
                     /locus_tag="J8V14_002207"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBA0922296.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="J8V14_002208"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="J8V14_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBA0922297.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="J8V14_002209"
     CDS             4450..5451
                     /locus_tag="J8V14_002209"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA0922298.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="J8V14_002210"
     CDS             5448..6809
                     /locus_tag="J8V14_002210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBA0922299.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="J8V14_002211"
     CDS             6769..7920
                     /locus_tag="J8V14_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBA0922300.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="J8V14_002212"
     CDS             7895..9064
                     /locus_tag="J8V14_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA0922301.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="J8V14_002213"
     CDS             9239..10618
                     /locus_tag="J8V14_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA0922302.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="J8V14_002214"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="J8V14_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2494 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DADQRX010000088        10822 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.090, whole genome shotgun sequence.
ACCESSION   DADQRX010000088 DADQRX010000000
VERSION     DADQRX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.090"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="J8V14_002215"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="J8V14_002215"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBA0922303.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2486)
                     /gene="purK"
                     /locus_tag="J8V14_002216"
     CDS             complement(1353..2486)
                     /gene="purK"
                     /locus_tag="J8V14_002216"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBA0922304.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="J8V14_002217"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="J8V14_002217"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBA0922305.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="J8V14_002218"
     CDS             complement(3374..4681)
                     /locus_tag="J8V14_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBA0922306.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="J8V14_002219"
     CDS             complement(4684..5265)
                     /locus_tag="J8V14_002219"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA0922307.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="J8V14_002220"
     CDS             complement(5837..7129)
                     /locus_tag="J8V14_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0922308.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="J8V14_002221"
     CDS             complement(7126..7749)
                     /locus_tag="J8V14_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922309.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="J8V14_002222"
     CDS             complement(7752..8588)
                     /locus_tag="J8V14_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0922310.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="J8V14_002223"
     CDS             complement(8605..9492)
                     /locus_tag="J8V14_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0922311.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="J8V14_002224"
     CDS             complement(9508..10791)
                     /locus_tag="J8V14_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0922312.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         3241 a         2455 c         1820 g         3306 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt tt
//
LOCUS       DADQRX010000089        10760 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.092, whole genome shotgun sequence.
ACCESSION   DADQRX010000089 DADQRX010000000
VERSION     DADQRX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.092"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..1042)
                     /locus_tag="J8V14_002225"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="J8V14_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="J8V14_002226"
     CDS             complement(1437..2279)
                     /locus_tag="J8V14_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA0922313.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="J8V14_002227"
     CDS             2604..3020
                     /locus_tag="J8V14_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBA0922314.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="J8V14_002228"
     CDS             3017..3487
                     /locus_tag="J8V14_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922315.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="J8V14_002229"
     CDS             3501..4274
                     /locus_tag="J8V14_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0922316.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="J8V14_002230"
     CDS             4288..5100
                     /locus_tag="J8V14_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA0922317.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="J8V14_002231"
     CDS             5154..6119
                     /locus_tag="J8V14_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA0922318.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="J8V14_002232"
     CDS             6255..8978
                     /locus_tag="J8V14_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922319.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="J8V14_002233"
     CDS             8991..9623
                     /locus_tag="J8V14_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBA0922320.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9828..10055
                     /locus_tag="J8V14_002234"
     CDS             9828..10055
                     /locus_tag="J8V14_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBA0922321.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10124..10390
                     /locus_tag="J8V14_002235"
     CDS             10124..10390
                     /locus_tag="J8V14_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBA0922322.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="J8V14_002236"
     CDS             10380..10649
                     /locus_tag="J8V14_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA0922323.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta
//
LOCUS       DADQRX010000090        10640 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.093, whole genome shotgun sequence.
ACCESSION   DADQRX010000090 DADQRX010000000
VERSION     DADQRX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.093"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1031)
                     /locus_tag="J8V14_002237"
     CDS             complement(183..1031)
                     /locus_tag="J8V14_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA0922324.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1195..2097
                     /gene="gnd"
                     /locus_tag="J8V14_002238"
     CDS             1195..2097
                     /gene="gnd"
                     /locus_tag="J8V14_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBA0922325.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="J8V14_002239"
     CDS             2099..3100
                     /locus_tag="J8V14_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBA0922326.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="J8V14_002240"
     CDS             3097..3381
                     /locus_tag="J8V14_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA0922327.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="J8V14_002241"
     CDS             3414..4211
                     /locus_tag="J8V14_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA0922328.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="J8V14_002242"
     CDS             4225..5058
                     /locus_tag="J8V14_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0922329.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="J8V14_002243"
     CDS             5076..5933
                     /locus_tag="J8V14_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA0922330.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="J8V14_002244"
     CDS             5955..6464
                     /locus_tag="J8V14_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922331.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="J8V14_002245"
     CDS             6501..6902
                     /locus_tag="J8V14_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922332.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="J8V14_002246"
     CDS             7014..7367
                     /locus_tag="J8V14_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922333.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="J8V14_002247"
     CDS             7401..7844
                     /locus_tag="J8V14_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBA0922334.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="J8V14_002248"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="J8V14_002248"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBA0922335.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="J8V14_002249"
     CDS             8939..9652
                     /locus_tag="J8V14_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBA0922336.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10404..10604
                     /locus_tag="J8V14_002250"
     CDS             10404..10604
                     /locus_tag="J8V14_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA0922337.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3552 a         1687 c         2310 g         3091 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9901 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
     9961 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10021 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10081 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10141 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10201 ttcgaagaca atagaagaca atgaaattta tatttcattg cattatcctt gtgttattag
    10261 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10321 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10381 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10441 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10501 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10561 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10621 accgtctttt cgaaagaaaa
//
LOCUS       DADQRX010000091        10604 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.094, whole genome shotgun sequence.
ACCESSION   DADQRX010000091 DADQRX010000000
VERSION     DADQRX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.094"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            13..636
                     /locus_tag="J8V14_002251"
     CDS             13..636
                     /locus_tag="J8V14_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922338.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /locus_tag="J8V14_002252"
     CDS             1086..2135
                     /locus_tag="J8V14_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA0922339.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="J8V14_002253"
     CDS             2132..3013
                     /locus_tag="J8V14_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922340.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="J8V14_002254"
     CDS             3016..3786
                     /locus_tag="J8V14_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA0922341.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="J8V14_002255"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="J8V14_002255"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBA0922342.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="J8V14_002256"
     CDS             5097..6260
                     /locus_tag="J8V14_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBA0922343.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="J8V14_002257"
     CDS             6377..6787
                     /locus_tag="J8V14_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA0922344.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="J8V14_002258"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="J8V14_002258"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBA0922345.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9327..9644
                     /locus_tag="J8V14_002259"
     CDS             9327..9644
                     /locus_tag="J8V14_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922346.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9804..10040
                     /locus_tag="J8V14_002260"
     CDS             9804..10040
                     /locus_tag="J8V14_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA0922347.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10037..10435
                     /locus_tag="J8V14_002261"
     CDS             10037..10435
                     /locus_tag="J8V14_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBA0922348.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3701 a         1771 c         2241 g         2891 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9721 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9781 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9841 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9901 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9961 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
    10021 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
    10081 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    10141 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    10201 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    10261 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    10321 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    10381 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    10441 ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct gattttttta
    10501 tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat taattttgaa
    10561 ataattgatt taaaaggaat acaaaagagt gaatagtagg aaat
//
LOCUS       DADQRX010000092        10549 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.095, whole genome shotgun sequence.
ACCESSION   DADQRX010000092 DADQRX010000000
VERSION     DADQRX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.095"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            412..660
                     /locus_tag="J8V14_002262"
                     /pseudo
     CDS             412..660
                     /locus_tag="J8V14_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            782..1108
                     /locus_tag="J8V14_002263"
     CDS             782..1108
                     /locus_tag="J8V14_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBA0922349.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            1176..1889
                     /locus_tag="J8V14_002264"
     CDS             1176..1889
                     /locus_tag="J8V14_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBA0922350.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            2199..3051
                     /locus_tag="J8V14_002265"
                     /pseudo
     CDS             2199..3051
                     /locus_tag="J8V14_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            3172..5037
                     /locus_tag="J8V14_002266"
     CDS             3172..5037
                     /locus_tag="J8V14_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0922351.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5085..6494
                     /locus_tag="J8V14_002267"
     CDS             5085..6494
                     /locus_tag="J8V14_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBA0922352.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            6602..7303
                     /locus_tag="J8V14_002268"
     CDS             6602..7303
                     /locus_tag="J8V14_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922353.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7462..7941
                     /locus_tag="J8V14_002269"
                     /pseudo
     CDS             7462..7941
                     /locus_tag="J8V14_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            8789..9364
                     /locus_tag="J8V14_002270"
     CDS             8789..9364
                     /locus_tag="J8V14_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBA0922354.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            9364..9804
                     /locus_tag="J8V14_002271"
     CDS             9364..9804
                     /locus_tag="J8V14_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBA0922355.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            9820..10521
                     /locus_tag="J8V14_002272"
     CDS             9820..10521
                     /locus_tag="J8V14_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBA0922356.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         3570 a         1713 c         2311 g         2955 t
ORIGIN      
        1 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
       61 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
      121 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
      181 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
      241 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
      301 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
      361 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
      421 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
      481 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
      541 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
      601 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
      661 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
      721 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
      781 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
      841 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
      901 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
      961 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     1021 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     1081 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     1141 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     1201 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     1261 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     1321 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     1381 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     1441 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     1501 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     1561 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     1621 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     1681 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     1741 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     1801 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     1861 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     1921 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     1981 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     2041 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     2101 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     2161 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     2221 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     2281 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     2341 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     2401 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     2461 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     2521 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     2581 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     2641 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     2701 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     2761 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     2821 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     2881 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     2941 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     3001 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     3061 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     3121 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     3181 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     3241 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     3301 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     3361 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     3421 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     3481 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     3541 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     3601 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     3661 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     3721 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     3781 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     3841 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     3901 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     3961 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     4021 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     4081 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     4141 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     4201 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     4261 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     4321 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     4381 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     4441 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     4501 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     4561 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     4621 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     4681 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     4741 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     4801 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     4861 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     4921 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     4981 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     5041 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     5101 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     5161 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     5221 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     5281 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     5341 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     5401 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     5461 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     5521 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
     5581 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
     5641 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
     5701 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
     5761 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
     5821 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
     5881 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
     5941 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
     6001 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
     6061 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
     6121 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
     6181 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
     6241 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
     6301 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
     6361 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
     6421 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
     6481 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
     6541 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
     6601 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
     6661 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
     6721 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
     6781 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
     6841 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
     6901 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
     6961 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
     7021 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
     7081 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
     7141 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
     7201 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
     7261 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
     7321 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     7381 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
     7441 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
     7501 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
     7561 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
     7621 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
     7681 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
     7741 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
     7801 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
     7861 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
     7921 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
     7981 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
     8041 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
     8101 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
     8161 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
     8221 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
     8281 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
     8341 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
     8401 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
     8461 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
     8521 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
     8581 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
     8641 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
     8701 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
     8761 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
     8821 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
     8881 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
     8941 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
     9001 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
     9061 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
     9121 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
     9181 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
     9241 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
     9301 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
     9361 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
     9421 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
     9481 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
     9541 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
     9601 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
     9661 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
     9721 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
     9781 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
     9841 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
     9901 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
     9961 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    10021 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    10081 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    10141 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    10201 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    10261 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    10321 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    10381 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    10441 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    10501 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaa
//
LOCUS       DADQRX010000093        10544 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.096, whole genome shotgun sequence.
ACCESSION   DADQRX010000093 DADQRX010000000
VERSION     DADQRX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.096"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..921
                     /locus_tag="J8V14_002273"
     CDS             <1..921
                     /locus_tag="J8V14_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBA0922357.1"
                     /translation="KEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKL
                     TISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQ
                     QKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANK
                     IQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRI
                     KELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEK
                     VVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            993..2114
                     /locus_tag="J8V14_002274"
     CDS             993..2114
                     /locus_tag="J8V14_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0922358.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2196..3968)
                     /locus_tag="J8V14_002275"
     CDS             complement(2196..3968)
                     /locus_tag="J8V14_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922359.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3971..5683)
                     /locus_tag="J8V14_002276"
     CDS             complement(3971..5683)
                     /locus_tag="J8V14_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922360.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5798..6466
                     /locus_tag="J8V14_002277"
     CDS             5798..6466
                     /locus_tag="J8V14_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBA0922361.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6533..7321)
                     /locus_tag="J8V14_002278"
     CDS             complement(6533..7321)
                     /locus_tag="J8V14_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBA0922362.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7404..8876)
                     /locus_tag="J8V14_002279"
     CDS             complement(7404..8876)
                     /locus_tag="J8V14_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0922363.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9006..10199)
                     /locus_tag="J8V14_002280"
     CDS             complement(9006..10199)
                     /locus_tag="J8V14_002280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBA0922364.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            complement(10465..10536)
                     /locus_tag="J8V14_002281"
     tRNA            complement(10465..10536)
                     /locus_tag="J8V14_002281"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10501..10503),aa:Glu,seq:ttc)
BASE COUNT         3508 a         2114 c         1731 g         3191 t
ORIGIN      
        1 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
       61 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      121 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      181 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      241 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      301 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      361 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      421 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      481 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      541 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      601 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      661 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      721 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      781 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      841 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
      901 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
      961 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1021 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1081 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1141 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1201 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1261 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1321 gtaatcttat acaagatatg ctagattact actattttga aaaaaattta tcatataatt
     1381 atgtaataca tttaaaaact tttacaggaa tgatttttaa attcgccaac cgtcgatatg
     1441 gattaaaata taatcccgta aatggggttt ctttagcaag aaaacctaag acaaacgaag
     1501 aaataaaaaa agaaaaagat ggttatttat caaaagaaga agttcaaatg attgctagta
     1561 aacaaaaaag ttcacatcaa cagtcacgtt attctttaat tactaaattt ttgtttttaa
     1621 ctggactacg ttatggtgaa ttgatatctt tgactgaatc tgattatgat ggtaataaaa
     1681 ttacggtttc tggtacttat gattacgagt taaaaattaa aacaacaact aaaaatacag
     1741 gttcttatag aaccattgaa ttatcaagta atgctaaaga aatcatagat caattaattg
     1801 aagaaaacaa attaattaag aataataaag ataaatacat tttcatctct aaaaatggta
     1861 acccgatatc tattcaagca tataatcaat ctttaagagc agtatccaaa gaattaaacc
     1921 tagataaaaa agtttcttct cacatgttaa ggcattccca catttctcta cttacagaat
     1981 taggaatccc attaaaagcc attatggatc gtgtaggaca tgaagattca aaaacaacat
     2041 taaagattta tactcataca actaaaaata tgcaaaatca actagtagaa aaactaggaa
     2101 aaatagaaat ttaaattgcc ccttttctgc cccttataaa aacaaacaac ataaaatata
     2161 taaaaagaac cccgttataa cgaggttctt ttaatttaac tttcaaatac aaattgatta
     2221 tgataaagtt ccgcataaaa tccattttct gctaataact cgtgatgact gccttcttca
     2281 atgacttccc catcacgcaa gacaacgatt ctatctgcat tcaagattgt tttcagccgg
     2341 tgagcaatca caaagctggt tcttcctttg attgcttcat ccattgcttt ttggatcctt
     2401 gcttctgtta ccgtgtctac attacttgtt gcttcatcca aaatcaataa agatggattt
     2461 gtgatgatcg ttcgagcaat actgatcaac tgcttttgac ctgtactgaa taagttattt
     2521 tcttcggata tctctgtatc ataacctttt tctaacgtca tgatgaagtc atgaatattc
     2581 gcttgttttg ctgcattgat gacttcttca tctgaagcgt ccggtttgcc gaatgtgata
     2641 ttgtctttga tcgttccgga gaacaataca gattcttgca aaacgattcc tacatgtcta
     2701 cgtaatgcat caagatcata atctcggata tctattccat cgatgactac tgaaccatca
     2761 ttgacatcgt aaaaacggtt tagcagattc atgatcgtcg ttttccccga tcctgttgga
     2821 ccaacaagag caaccatttc tcctttgtcc acattgatcg agacatcttt caagactagt
     2881 ttttctggag aataaccaaa atctacgtgc tgcaacgcaa gacctttttc aacaccatca
     2941 aatgctttac agttttctgg tttgacttca tctttttcat caaatatttc attcaatcgt
     3001 cttgcccctg taatcgctaa ttggatcatg ctgtatccag atgaaatctg catcagtggt
     3061 tggtaatatt gttgtgaata ctggacgaat gtcacaacta agcctaatgc caccgaacgt
     3121 tccaaatcac catttaatgc taaccagccg ccgaaaaaga taactatcgc tgtattgacc
     3181 agtgacattc cttgcatcat cggaaatagt agaccagaat aagcttgtcc tttgaaggtt
     3241 gcttcgcgaa cactttgatt atgctctaag aatccttcaa tcgtttcttc ttgtaagcca
     3301 tttgtgatga tgacacgttg tccgctgatt ttttcatcca tataaccatt tagtttcccc
     3361 acttcatctt gctgaagatc cacatatttc cgtgctttgc ggataatcaa gacagctatc
     3421 aaaacggcta ctggagtgga agcaattgta gcccaagcta actctacatt ttggcggaac
     3481 atcatgatca agatcccgac aagtaaaaca gcattcgtga tgacttgaag caaagcttgg
     3541 ttcaaactat tttgaatatt atctaagtca ctggtaaaac gacttaaaat ttcaccgtct
     3601 tgatgagaat caaagaaacg gatcgtcaat ttttccaatt tgttgaatag accgatacgc
     3661 attcggttcg ttgacttccc tactacttgt gtgaacaaaa tactatagat aaagttggct
     3721 gcactagcta gtacataaaa cagcaagagt ttccatatta cagaaacaaa tttgctttta
     3781 tcgtctacgc cttgcatcaa gcctcctacg taattagcta attcttgaaa agcttccccg
     3841 atgtattgag gtgcctttac ttgtagatag gtagcaatga tcactgtgat aaagataaag
     3901 aaaaaggaga gtttgtaccg cttcaaatag tgatagaaaa atttacttgc ttttattaga
     3961 tcagtcatac ttactcctcc gttccttttt gagtttcaaa gatttcctga tagacacggt
     4021 ttccagctac caattcctca tgggttcctt cgccgactaa tctgccttca tccaatacta
     4081 agatgcggtc tgctttgaca acagaagcaa ttttttgtgc gatgacaatc gtcgttgttc
     4141 ccttcaaatc tcgatcgagt gcttctcgga ccagcttttc agagcgagca tccagtgcac
     4201 tcgtactgtc atctaggatc aagatctttg gattaccgat aacgccacga gtaatggata
     4261 aacgttgctt ttgaccacca gaaaagttac tgctacgttc ttctattggt gcttcgtaac
     4321 gatcagataa cttttcgatg aattcttttg cttgagcaat agaagaagca tgttccatct
     4381 ctttttctgt tgcattcttt ttcccttggc gcaagttttg agcaatcgtt cctgagaata
     4441 aaattgcttt ttgcaaaaca aaggaaaccg tttgtcttaa actcttttca tttacttgac
     4501 gaagatctac accacctact tcaactttgc cttctgtcgg atcaaataga cgagggatca
     4561 attgtgctag tgttgacttc ccggcaccag tcgccccgac gataccaacc atttctccag
     4621 gtttgataga aaagctgata tctttaagcg tatctgtatc ctcaccagga taacgaaaag
     4681 aaacatggtc aaaagtgaca gaacccgtca actcttgttc tggaacgtct aaataagtaa
     4741 gatctggttc agcatctaaa atttccgcaa tccgcttcag cgatacggct gctcttgaag
     4801 tcatcatcat catcattccg ccaataatga ttgccatcat aatctgcatc aagtagttca
     4861 tgaatgaagc aattccacca attaacgtag gatcatcttt aacaagatcg ctcacaaaaa
     4921 agatcgcccc aaccacagcc aaattggcag ccagcatgaa ggacgggatc atcacagaaa
     4981 ataatgtacc gactatgatg ttgtgttttg ttaagtcctc acttactttg ctgaatgact
     5041 tcaattggtt ttcttcttgt acaaaagatt tcactacacg aatccctagt aagttttctt
     5101 ttgcaatgct attcacacga tcaatcagtt tttgaatgat catgaagtgc ttgcccatcc
     5161 ttgtaaatga aagtgccgta atcagaaata ccgtaacgat aagcgctacg atgatccacc
     5221 ataattgcgg catcgtgatc atagctaaaa tgaaggcacc aataaataag aatggaattc
     5281 gaaaaagtga ttgcaaagaa atcatcacta aattttggat ttgcgtgata tcatttgtta
     5341 gacgaacaac aagatttcca gcagagaact gttcgatatt tccaaatgaa aatgtctgga
     5401 tctttcgaaa tgcttcttct cggatatctg cacttacgcc ttgagctact tttgcagaga
     5461 aaatcgtgtt cgttacacct gcggctaatc ctagcaaagc cagactgatc aggtaaatac
     5521 caattgtttt catttcatca ttatcttctg taataatagc ttccaatact tgctgtagta
     5581 acttaggttg ccataatgct gctgcaacca tgactgctac ggacagcaag gaaataaaaa
     5641 cagctacttt atattttttc gcatgtcgta agattaattt cattcgttga ttacacccct
     5701 ttgtttaaat tgacaggact aatttaacac tgtaaaatta gaccgactaa caatttacca
     5761 tgtttaaaaa gataatgcaa tacacgaggt tgataaaatg gaaataaagc tttctcaagc
     5821 aatcatcatt caggccgcct ttgatatcct ggctgaaaca caagctcttt cgcagctttc
     5881 catgcgaaac atcgcaaaca gaattcatgt tcaagcacct gctttatatt ggtatttcaa
     5941 aaacaaacaa cagctcttgc aaataatggc tgaaacaatg gaagaagaat tagcgcttcc
     6001 agatccaact ttgccttgga atgaacagtt gctgcagtat atggaaaatt actatgacct
     6061 ttatacacgt ttcccttgtg gggcagaatt agaaatcaat actgtgcccg cttttccgag
     6121 tcgattagaa catttggagc aaatgatcca attacttgtt cgtgaaggat tttcagtcac
     6181 ccaaagtcat caggccattg cctctctcca caatttattg attggacagt taatggatca
     6241 gcagcatgaa cagcagcttc gacaaaaaat tatggacgga aatgaatttt tgaaagaaac
     6301 agttctattt atgcgtaatt atgtagttga aaatcaatta gagggattta aagaaagtat
     6361 gcagtcccat gcagaattga atgaaaaagc actctttcta acgagtgcac aaacctatat
     6421 tgaaggctta tgccaacaaa ataaaagaag agaaaacgag cagtaaaaaa gctcctatag
     6481 agatcactat gtttgtgatc tctataggag ctatcttttt aagactcttt ttttattttt
     6541 cttccattag ccacgccaat gcacgttgaa tccattggtc ccagaagatc cagttatgat
     6601 cgccaggtcc ttgttcaaag gtgaccgcca agccttgttc ctgcatagca gataccattt
     6661 cctgatttgc ctgataaaga aaatcagatt ccccacaagc catataaaat cttggaactg
     6721 gttttccgct ttttactagt tctttcaata agtattgcgg atcatttttt gaaccttcga
     6781 tttgatccaa tggtccaaag atcccttccc aaaaagatgc tttacgaacc atcaaaagat
     6841 catccattcg atttcctaca gaaacagcgc ctgaaagaga gattactgct gcgaaatttt
     6901 ctgattttgc aaggcctagt tttaacgcgc catatccgcc catcgataat ccagcggcat
     6961 atgttttttc tctttttctt gttaattgcg ggaataattc atgacaaatc tcaggtagct
     7021 cttctgcaat gaatgtccaa tacttcatgt cataaactgt atctgtgtac cagccaagat
     7081 cagtagatgg catgatgaca gctaatccgt agtttgctac ataacgttca atcgacgtac
     7141 gtctttccca tacactatga ttgccaccca ttccatgaag taaatataaa acaggtatat
     7201 ccgttgtctc ttgctctgaa ctggtgccaa tcaccttttt tgttgtttgc ggtagtatca
     7261 catttagatt gacttccatt tctaacacat tcgaataaat atttgcttga ataaatgcca
     7321 tattttcgcc ttccttagta agaagttatt tttgaataac tttaaatttg aatgattcgg
     7381 ctacgtacat atcttaaccg aaactactgt tttattttac cacttttttc atgaacgaaa
     7441 agcgttaaga ccaaagcaat gatcacgagt aacaagccaa ctagataaac ggaatgtaac
     7501 ccatcataaa gaatttttct taatggtaac aaaatatccg catcaatata tttagccgtt
     7561 tgtggattca ccagtttatt catgatatct gcatcatttg ttaaatttgc tgctgcaagt
     7621 tctcgtttag ttaccccatt tatcaataaa ccaaaaatag aaaccatgac tgtttgacca
     7681 attgttcgaa ctaacgtatt aaaagaagtt gctacaccaa gctggtcacg agatacacta
     7741 ttttgtgcac tgacagtcga ggtagtcatg gtcacaccta agcctatacc gatgaccgtt
     7801 gatatagcaa aaaaccataa atagttactt ctgaaaggca taagatacaa ccaagcgccc
     7861 ccaatagctg cgattagtag tccaattctc aagacccaat gagttttcat ttgagtcatc
     7921 cacttgcttg caaggaaaga accgaccatc cagactagtg ataatggtgc taataccagc
     7981 ccaccaattg ctgcattcag ccctaataca ccttgcatcc acatagggat ataaacatcg
     8041 attcccatca aaaaaccact agccaatgct gcagccaagt tgaccacaac aaatagtgga
     8101 tttttgaata aatgcatcat gatgacagga tctttagctc ttttttcggc aaaaacaaaa
     8161 atacctaaca ttacgatact acctagcagc aaagcagaaa caagcggact cattccttcg
     8221 tctcctaata attggaaccc taacagcaat gagacaagca tggccatcaa actaaaacta
     8281 ccaaaaatat ccattggttt agactcatgt atgatttttg gttcatttaa atatagccaa
     8341 atcaaaataa ttaatagaat tccgatcggc acattgataa aaaagatcca gtgccagctg
     8401 atcgtttcaa cgatgaatcc tcccgcaagc ggtccaaaaa cactggcaat cccccaggct
     8461 gaactattca aacctaaaac ttttgctcgc ttttcaatag aataaagatc gccgatgatc
     8521 gtcaaagcta ctggcatcat tgccccagca cccatccctt gaatcgcacg agcagcaatc
     8581 aacccaatca tgctttgaga taaaccgcaa aataacgaac cgataataaa caataatgtc
     8641 ccaaaaataa agattggttt ccgtcccact ttatccgcaa gttttccgta aatcggagta
     8701 agcatagcgt ttgtcagcaa ataaatcgaa aatacccagt tcatgatttc cattccatgt
     8761 aatgaaccga caatcgttgg catagcggtt gttacgattg taccttcaat agccgtcatg
     8821 aacgttgcaa tgaaaatact cactgtgacc aaagtaacat ttgtttcttt tttcatctta
     8881 ttcctccctt tcattatgac aggatctaaa aatagataaa taattactat ctttttcaag
     8941 aggttaactg ccataaaaaa agctggaaca accaccactt ggcgatcgtt ccagctgaat
     9001 ataacttatt ttgctaaact tgctttcaac gcatctactt tgtccgtatg ttcccaaggc
     9061 aaatctacat ctgtacgacc aaaatgaccg taagcagctg tttgtttgta aatcggacga
     9121 cgcaaatcaa gcatctcgat gattcctgct ggccgaagat cgaagttttc tctcactgca
     9181 gcaatcagtt cttcttccgg aaccgttcca gtaccgaaag tattgattga gatcgaaaca
     9241 ggttgagcaa caccaatcgc ataagctagc tgtacttctg ctttgcgagc aagccctgct
     9301 gcaacaatgt ttttagcaat ataacgtgca gcatagctgg cagaacggtc aacttttgtc
     9361 gcatctttac cagaaaaagc accgccacca tgacgagcat aaccgccata cgtatcaaca
     9421 atgatttttc ttcctgttaa tccggcatcc ccttgaggac caccaatgac aaatcggcca
     9481 gtcggattga tataatattt cgtttgatca tctaataatt cagctggaat aacttccttc
     9541 actacttttt cgatgacatc atgacgaatt gtttcattat ctactgcatc atcatgctgt
     9601 gttgaaataa cgattgtatc tacgcgttcc ggttgtcctt gatcatcata ttcaaccgtt
     9661 acttgagatt ttgcatctgg acgtaaatac gttaattcat ttgatttacg caaatctgcc
     9721 aagcgtcgta ccagacgatg actcaaagcg attggtaaag gcatcaattc tggtgtttca
     9781 tctacagcaa acccaaacat taaaccttgg tcaccagcac caatctcatc taatacgtct
     9841 tttttatctt catcccggat ctcaagtgct tcatcaactc cttgagcaat atcaggagat
     9901 tgttcatcaa tcgcaaccaa tacagctgct gtatctccat caaatccgaa ttttgcacgt
     9961 gtataaccaa tttcttttat tgtttcccgt acgacttttt gaatatctac atatgccgtc
    10021 gtagatattt ctccaaatac taaaacaaga ccagttgtta cagacgtttc acacgctact
    10081 cgtgctgttg gatcttgttt taaaattgca tccaagattg catcactgat ttggtcagca
    10141 attttatctg gatgtccttc agaaacagat tctgatgtaa ataagtgtct ttctaccatt
    10201 ataatgattc cccctagtta aattcggtta caaggcatct ccgtcctgcg gatcctttcg
    10261 ggaacttgca acgaaccaag tatagcagat aaaacagttt tctgctcgta ttttttcatt
    10321 tttttgctta aaaaatataa aaatatttta cattagaact gtccaaaaaa gcttagcaca
    10381 atcatccata aagataactc tgctaagctt tttaaaatca ttctttcaga taaaaagaaa
    10441 aagatactca aaaagtatct tccatgaccc gtacgggatt cgaacccgtg ttaccgccgt
    10501 gaaagggcgg tgtcttaacc gcttgaccaa cgggccaata aaat
//
LOCUS       DADQRX010000094        10536 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.097, whole genome shotgun sequence.
ACCESSION   DADQRX010000094 DADQRX010000000
VERSION     DADQRX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.097"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..1666)
                     /locus_tag="J8V14_002282"
     CDS             complement(107..1666)
                     /locus_tag="J8V14_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBA0922365.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1821..4055
                     /gene="pcrA"
                     /locus_tag="J8V14_002283"
     CDS             1821..4055
                     /gene="pcrA"
                     /locus_tag="J8V14_002283"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBA0922366.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4072..6108
                     /gene="ligA"
                     /locus_tag="J8V14_002284"
     CDS             4072..6108
                     /gene="ligA"
                     /locus_tag="J8V14_002284"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBA0922367.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6214..6519
                     /gene="gatC"
                     /locus_tag="J8V14_002285"
     CDS             6214..6519
                     /gene="gatC"
                     /locus_tag="J8V14_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBA0922368.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6519..7991
                     /gene="gatA"
                     /locus_tag="J8V14_002286"
     CDS             6519..7991
                     /gene="gatA"
                     /locus_tag="J8V14_002286"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBA0922369.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7988..9418
                     /gene="gatB"
                     /locus_tag="J8V14_002287"
     CDS             7988..9418
                     /gene="gatB"
                     /locus_tag="J8V14_002287"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBA0922370.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9428..10483
                     /locus_tag="J8V14_002288"
     CDS             9428..10483
                     /locus_tag="J8V14_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBA0922371.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
BASE COUNT         3596 a         1973 c         2310 g         2657 t
ORIGIN      
        1 aaaaaagaag aggctgggtt ttcccagcct cttctttttt tatagaatca tatagaatca
       61 agtagtgtag atgaatagtt gttgtaaaat agtttttcca tgttcgttaa aatgattgct
      121 tcaaatttgt gatagaagat ttagcctcga tttcctgctt gattcttggc aagtttgctt
      181 catcagtcaa gataaccaaa atatctccta cattcatgat cgtatcaccg tgagtcaaga
      241 tttcctgatt tcctctgcgt atagagatta gcagcatatt tttaggccaa ttgaagtctc
      301 gtaccattaa tccgtctaaa gaactctctg ccgttactgg aaaatcaaaa gttgtccttg
      361 caccttttag tgaggcggtc tgagtatttc caagtagacg ttccaataat gattcgtaaa
      421 ttggattacc gccgagaagg tcattgacga tataagcaac aagagcagcc aatcctaaag
      481 gcatcagatg attaagagag ccaaccattt ctgtcactaa aataattgct gtcagcggtg
      541 cttttccaat cgctgtgaaa taaccagcca tggcaataac gacaaaatcc ttgatgtaga
      601 ttggatccat cccgaaatca accataatgt ttccataaaa gctaccgatt aaagccccca
      661 atgtcaatat cggaaggaaa atcccccctg gaagaccccc accgtaagaa atcatggaaa
      721 aaacgaatcg aagaacaaac aaaccgataa tcataatgat tggcaaggaa gcatgtccaa
      781 aagctaaaac gatttgattg cctccgccta gagtactagg ccataaataa ccaattggca
      841 aaatcagcaa gaaaggaacg aacccgtgaa aataagctgg taaaggcaat cggttgtaaa
      901 attttggcaa agccagcaag acattctgat aaataaaacc taagatgcct agaagtactc
      961 ctaagatcac taaggaacca taatatttca acggcaaaga aggaaggtca cctagatata
     1021 aaacaggtct caagccgaaa aagtaaagag agacgaagtt agcagtgatt gctgaagcaa
     1081 atgatgtcag ccaaacaagt ggagaaaagg agtgatggat ttcttctaaa acaaagagta
     1141 atcctgcgat aggagcattg aatgctgctg caagtccgct acttgcaccg ctagaaatta
     1201 aaatcttttc ttctgaagat ggactccgaa acaattgtcc agctccttgt ccaatggatg
     1261 cgccaagctg tatagaaggt ccttctctac ctagaaaaag gcccgcacca actgaaagta
     1321 caccgccaat gaactttttc cataaaacag agaaccagtt cattgataat tccccgcgaa
     1381 gctgtcccac tacttgtgga atcccgcttc ccttgatatc cggttcattt ttgaccagat
     1441 aacccacgaa aaatgctgca aggatgcagc aaatagctag gccaattaag taaatcggct
     1501 gttcccgact catttcatag atacttcgaa ctaaatcgct tagcttttcg atagataatc
     1561 gaaacaggct aacgacgatt cctgtcagca gtccaacaaa tattcctttt agaataaaat
     1621 atatctgagt cttatctaat ttcttgattt ttcctttttt ttccattcaa acgcctcgct
     1681 ttcttttaat ttagcgcgaa atcaacgaat tgacaaggaa aaatccataa aaagaaacaa
     1741 atgattttga actagctagt ttaatgaaag ccaagaaaat gctataatta acaagattga
     1801 taaaagaaag gatgttgtta atgtcatcca aagctgaatt attaaacggg atgaaccccc
     1861 gtcagaaaga agctgttctt cacactgacg gaccattact gctaatggca ggtgcaggaa
     1921 gtggaaagac gagagtgctg acacaccgca ttgcctattt gattgaggaa aaagaagtca
     1981 atccttggaa catcctagcg attactttta ccaataaggc tgcaaaagaa atgaaagaac
     2041 gtgtcaatgc gattctcgca tctggaggcg aagatgtctg ggtatcgact tttcactcga
     2101 tgtgtgtgcg tattttaaga agagatgttg attttatcgg gtataaccgg aactttacaa
     2161 tcatcgattc ttctgaacag ctgaccttga tgaaacggat actaaaagaa ctgaacattg
     2221 atccgaaaaa atatgatcct cgaagtatat taggaacaat ctctcaagca aaaaacagcc
     2281 tgcagacccc tcaggatttt gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa
     2341 aatgttatgc tgcttaccaa aaagagcttc aatataatca atgtatggac tttgacgacc
     2401 tgatcatgaa tacgattcga ttatttgaag aacatcctga ttcactcacg tattatcaaa
     2461 acaaattcca ttatattcat gtagatgagt accaagatac aaatcacgca cagtatacgc
     2521 tagtcaattt attggcaggt agatttagga acctgtgcgt cgtgggagat gcagatcaaa
     2581 gtatttacgg ctggcgcggt gcagatatgc agaacatctt ggattttgaa aaagattatc
     2641 cagatgctgc agtaattttg ctagaacaaa actatcgctc cacgaaaaac atccttagcg
     2701 cagcaaacca agtcattgaa aacaatagca atcgaaaacc aaaaaacttg tggacagaaa
     2761 acaaagaagg aaacaagatc acttactacc gtgcagataa tgaacgagac gaaacccgtt
     2821 ttatcgtaga ccggatgcaa gaagagattc gaagcaatca tcggaattac ggcgactttg
     2881 ccatcttgta ccggacgaac gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa
     2941 atattcctta taaaatggtt ggagggcaca agttctacga tcgtaaagaa atcaaagata
     3001 ttttagcgta tttgaatgtg ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg
     3061 ttaattcgcc caaacgaggc atcggaccag gatctatcga aaaactacgc agttttgctt
     3121 ctcttcatga atggccactg cttgaagcag cgcaaaatgt ggatctggca aatatcggtg
     3181 gaaaagcagg acagcaatta ggtgcatttg gagagatgat ccaagaagtc actcagatga
     3241 tcccatactt gacagtgaca gaattgacga aagaggtttt ggatcgtagc ggatatctag
     3301 aagacttaaa aatccaaaat acattagaag cacaagcacg catcgaaaac ttggaggaat
     3361 ttttaacggt cacacaagaa ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg
     3421 atgcaccaga agaaaaacta acggtcttct taaatgatct ggcacttgtt tccgacattg
     3481 acaatttaga agaagatgct tcccaagtga cattgatgac tttgcatgca gccaaaggat
     3541 tagaatttcc agttgtattc ctgattggtc tagaagaggg ggtttttcct ttatctcgag
     3601 cattgatgga agaaagcgaa ctggaggaag aacgccgtct cgcatatgtg gggatcacgc
     3661 gagcagaaga agcgttgtat ttaacaaatg ctttctccag aacattatat ggacggacgc
     3721 aatataaccg accttctcgt tttgtagaag aaatcgatca ggagttactg gaaatcgaag
     3781 gcatgcgtcc tacgcctaaa aaaacgcctg tattcgctaa aaaaacagca tatagctata
     3841 aacagccaga gacagcggtt gttccctcta aaagtgctac tggaggggaa aataacagct
     3901 ggaaacctgg agataaagtc aaacacaaaa aatggggtct aggaacagtc gtccgagtaa
     3961 gcgggacgtc gaaggatcta gaactagatg tggctttccc aagtcaagga gtaaaacgtc
     4021 ttttggctgc ctttgcgcca attgaaaaag cttagtagtg gagggtaaat gatggaacat
     4081 gaaatgacga ttgacgaagc tgctaaacga gcagaagaac ttcgtacaag actgaaccaa
     4141 tggtctcgcg aatactacgt agaagataaa ccaacagtgg aagactatgt ttatgataaa
     4201 gaatacgcag aattagtagc aatcgaggaa caatatcctg atttgatcac gtctgattct
     4261 cccactcaac gagtaggcgg aaaagtatta gaaggttttg aaaaagtcac tcatgatatt
     4321 ccgctttaca gtttgaacga cgttttcagc aaagaagagc tgatagcttt tgatcaacgt
     4381 gtacaaaaag cagtgggacg tgtagttgac tattgctgtg aactaaaaat agatggactt
     4441 tccgtttctt tgcgttatga agatggaaat ttcgtgagag gtgcgactcg cggggatgga
     4501 actgtaggag aaaacatcac tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta
     4561 aaagaaccaa tgaacatcga agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc
     4621 caattgaacc aagacagaga agcagaggga aaagatatct ttgcgaatcc tcggaacgcc
     4681 gcagctggtt ctttgcgtca gttagattct aaaatcacag cgaaaagaaa tctggacact
     4741 tttttataca cagtagctga tttcggtcct atgcaagcaa aaacccagta tgatgcgcta
     4801 gaagagctag aaaaaattgg attccacacg aatcgggaaa aacgtctatg tcattcgatt
     4861 gatgaagtgt ggtcatacat tgaagaatat catgacaaac gtgtggattt gccctatgaa
     4921 atcgatggaa tcgtcatcaa agtgaatgaa ttctcgctac aagatcaact tggttttact
     4981 atcaaagcac cgagatgggc agcagcttac aagtttccgc cagaagaagt tgaaacgcta
     5041 atcgaaaaca tcgaatggac agtcggtcga acaggggtag taacgccaac agctatcatg
     5101 acaccagtcc gagtagcagg aacgacagta agccgtgcca gcctgcataa cggtgattat
     5161 attaagttga aggatatccg cttgaaagat acagttctga tttataaagc gggagacatc
     5221 atcccagaag tttcgcaagt cgtgttagat aaacgaccta aagacagcga agaataccaa
     5281 ttgcctacac attgtccagt ttgtggaagt gaacttgtcc atttggatga agaagtagcc
     5341 ttacggtgca tcaatcctaa atgccctgct caaatgaaag aaggtttgaa ccactttgtt
     5401 tcaagaaatg cgatgaatat cgatgggttg gggccgcgcg tactagaaca gatgtatgat
     5461 aaaaaactag tagcagatgt tgctgatctt tacaaattga cagaagaaga gttattgacg
     5521 ttggacaaaa taaaagaaaa atcagcaaat aatattttga cagcaatcga caacagcaaa
     5581 gataattcgg tcgagcggtt gattttcggg ttaggaatcc gacatgtcgg agcgaaagca
     5641 gcaaaaattt tagcagaaca tttcggtgat ctagaaacat tgagtaaaag tgactatgag
     5701 tcgattattg cgcttgatac gatcggtgat atcattgccg acagtgtagt cacttatttc
     5761 agtaacgaag aagtccatga attgatgaat gaattaaaac aagcaggcgt caatttcgaa
     5821 tacaaagggc ttcgaaatgc ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca
     5881 gtagtcctta ctggtaaact aacacgattt actcgtgaag aagcaaaaga gacgatcgaa
     5941 aatcttggcg ggaaagtgac tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga
     6001 gaagatgctg gcagcaagct gaccaaagca caagaattag gaatcgaagt ttggacagaa
     6061 gatcaaatgg cagatgcatt ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt
     6121 ttcagacaaa tttcaataaa caatttcaga cacggcactc ttgtgttaaa atagagagca
     6181 aagtttttaa ctcgtttaga aagaaggggt agtatggcga ttagtgaaga acaagtgaaa
     6241 cacgttgcca aattagcaaa acttgcgttc gcagatgaag aacttgcaag ttttaccgac
     6301 caattaggta aaatcatcga tatggttgaa atgctggaag aagtagatac ggaaggtgtt
     6361 ccatttacgt cgaatgtggt tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct
     6421 ggatggaacc gcgatgaatt attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa
     6481 gtacccgcaa tcatcgataa tggggaggct ggcgcataat gacagaatta cacaatcgtt
     6541 ctctagaaga actgcatcat atgcttgtat cgaaagaaat cactgtacaa gatttgacaa
     6601 aaacaacatt tgaacgaatc aaagaaacag agcctgaggt cgaggcattc attacattga
     6661 acgaagaaaa agcattggaa caggcaaaag ctttagatga aaaagggatt gatgaaagca
     6721 acgtccttgc tggtattcca attggtatca aggataatat cgtaacaaaa gatatcttga
     6781 caaccgctgc atctaagatc ctttacaatt ttgaaccaat ttatgatgca acggtcatgg
     6841 ataaaatcta tcaagcagat atgatccctg ttggtaagct aaacatggat gaatttgcta
     6901 tgggtggaag tacagaaact tcttatttca agaaaacaaa aaatgcttgg gacccaacaa
     6961 aagtacctgg tggctcttct ggtggttctg cagcagcagt tgcagctgga caaattccag
     7021 tttctttagg tagtgatact gggggaagta tccgccaacc agcagctttt aacggcatcg
     7081 tagggatgaa acctacttat ggccgtgtct cacgttttgg tttgattgcc tttgcttcat
     7141 ctcttgacca aatcggacca atgacccgta cggtgaaaga taatgcatta gcattgacag
     7201 ctatcagcgg ttatgacgaa aaagacggta cttcttcagg cgtttcagtt cctgattttg
     7261 cagagggact gacaggcgac atcaaaggaa tgaagattgc tttacctaaa gaatacttag
     7321 gcgaaggtgt ggcccctggc gtaaaagaag ctgtattgaa agcagcagaa acattccgtt
     7381 cattaggtgc tgttgtggaa gaagtcagct tgcctcattc aaaatacggt gtggctgttt
     7441 attatatcat tgcctcatca gaagcaagtt ccaatttgca acgattcgat ggtatccgct
     7501 acggttatcg ctctgaaaat gtgaaatcat tagatgatgt atatgtcaac tcacgttcag
     7561 aaggattcgg tgaagaagta aaacgccgta tcatgttagg tacattctcc ttaagcgcgg
     7621 gttattatga cgcttacttc aaaaaagcag gacaagtccg tacattgatc aaacgagatt
     7681 ttgaaaaagt ctttgcagat tatgacttga tcatcgggcc atcttcacca acagttgcat
     7741 tcggaatcgg tgagaacatt aacgatccaa tcacaatgta catgagtgac atcttaacga
     7801 tccctgtcaa cttggctgga cttccaggta tgtctgtacc ggcaggttta tcagaaggat
     7861 taccagttgg attgcagatc atcggtaatt attttgatga aaaaacgatg tatcaagcgg
     7921 catatgcgtt tgaacaagca acagaattcc ataaacaaca accagctatt ttaggaggga
     7981 aagacgaatg aattttgaaa cagtcattgg actagaagtc cacgtcgagt taaaaaccaa
     8041 ctcaaaaatt ttctctccag cgccagctca ttttggtgca gaaccaaaca gcaatacaaa
     8101 cgtgatcgac tggggttatc caggcgtgct gccagtaatg aataaagctg cgctggaatt
     8161 cgggatgaaa gcagctttgg cattaaactg tgaaatctct aaagatactc attttgaccg
     8221 gaaaaattac ttttacccag ataatccaaa agcatatcaa atttcccaat ttgatcaacc
     8281 aatcggtcat gacggatgga tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat
     8341 cgaacgtgtc catttagaag aagatgctgg aaaaaacatg catggtattg gcggttattc
     8401 ttacgttgat ttgaaccgac aaggcacgcc tttgatcgaa atcgtttctg aagctgatat
     8461 gcgttcacca gaagaagcat acgcttatct agaagcattg cgatcaatta tccaatttac
     8521 agaagtcagc gatgtgaaaa tggaagaagg ctctatgcgc tgtgatgcca atatttctct
     8581 tcgcccttat ggacaagaag agttcggtac gaaagcagaa ttgaaaaact tgaactcttt
     8641 atctttcgtg aaaaaaggtt tagcttttga agaaaaacgt caagcaaaag ttcttctttc
     8701 aggaggagaa atccaacaag aaacacgccg ttttgacgaa acaacgaata aaacagtttt
     8761 gatgcgtgtc aaagaagggt ctagtgatta tcgttacttc ccagagcctg atatcccgaa
     8821 attcgtgatc gatgacgaat ggattcaaaa agtaaaagct agtttgcctg agatgccagc
     8881 ttctcgtcgc gaacgctata tccgtgagtt tggacttcca gagtatgacg caatggtatt
     8941 gacacttaca aaagaaatgt ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc
     9001 caagcaagca tctaactggt tgatgggtga agtttctgct tacttgaaca gtgagaaact
     9061 ggaattagct gagacaaaat tgacacctga gaacttagca ggaatgattc gtttgatcgc
     9121 cgatggaacg atcagctcaa aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg
     9181 cggagaagcc caagaagtag ttgaaaaaaa tggttgggtg caattatctg atccgaacaa
     9241 actactgcca atcattaatg aaattttgga taacaaccaa caatctgtcg atgatttcaa
     9301 aaatggaaaa gatcgtgcgg ttggcttcct agtcggtcaa atcatgaaag caactaaagg
     9361 acaagcaaat cctggtgtcg tgaataagct acttaaagat gaattagcga aacgatagag
     9421 gctgaaaatg aaaaaagcac gcgtaattta taaccccacc tcgggaaaag aattattgaa
     9481 aaaaaatttg gcggatatcc tacaggcatt agaagaagca ggatttgaag ccagcgccta
     9541 tgcaactaca ccagaaccag attcagccaa gaatgaagca tgtagagtcg cagaactagg
     9601 ttttgatcta gtagtggcag ctggtggcga tggcacaatc aacgaagtgg ttaatggaat
     9661 cgctccgctt gaacaccgtc ctaaaatggc catcatccca gctggtacaa cgaacgacta
     9721 tgctcgagca ttaaaaattc ctagagataa tattaaagca gcagcagaag tgataaaaaa
     9781 acaccaaacc gtaaaaatgg acatcggcct ttctgataac agttacttca tcaatatcgc
     9841 tgcaggtggc tatttgacag aattgactta tgaagttcca tcagaactaa agagtatttt
     9901 tggttatctt gcttatcttg cgaaaggagc agagatgctc cctcgagtaa agccaatcaa
     9961 aatgcgcttg aagtatgatg aaggggaata tatcggaaat gcttcgatgt ttttcctagg
    10021 gctgacgaac tctgttggag gattcgaaca gatcgcgcca gatgccaaat tggatgatgg
    10081 gaagttttct ttaatcattg tgaagacagc aaatatcttt gagatcttgc atattgcagc
    10141 actcatgctg aatggcggtc gacatgtcga tgatccaaga ataatctata ccaaaacaag
    10201 cagtttgttc gttgaaacag aagatgatat caatcgacca gtaatgatca acttagatgg
    10261 tgaatacggc ggggaagcac cgatgcattt ccaaaatctt catcaacaca ttgagttctt
    10321 tgccaatacc gatcagattc ctgatgaagc aatcacaggt acagatgaag aagaattaga
    10381 ggttgtcagc aaagaattcg taaaagaagt cgaacgttta acggatgaag atatcgacgg
    10441 tgacggaaaa atagcagacg aaacacctaa tcaaaagaat taatcaaaag ccggtgctga
    10501 acgtagtaag gtcagtgccg gttttagttg tgtaat
//
LOCUS       DADQRX010000095        10266 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.098, whole genome shotgun sequence.
ACCESSION   DADQRX010000095 DADQRX010000000
VERSION     DADQRX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.098"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..1568)
                     /locus_tag="J8V14_002289"
     CDS             complement(60..1568)
                     /locus_tag="J8V14_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBA0922372.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1727..1918
                     /locus_tag="J8V14_002290"
     CDS             1727..1918
                     /locus_tag="J8V14_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922373.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2121..3038
                     /locus_tag="J8V14_002291"
     CDS             2121..3038
                     /locus_tag="J8V14_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBA0922374.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3031..4104
                     /locus_tag="J8V14_002292"
     CDS             3031..4104
                     /locus_tag="J8V14_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922375.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4252..4770
                     /locus_tag="J8V14_002293"
     CDS             4252..4770
                     /locus_tag="J8V14_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922376.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4751..5143
                     /locus_tag="J8V14_002294"
     CDS             4751..5143
                     /locus_tag="J8V14_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBA0922377.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5219..6154
                     /locus_tag="J8V14_002295"
     CDS             5219..6154
                     /locus_tag="J8V14_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBA0922378.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6287..7066
                     /locus_tag="J8V14_002296"
     CDS             6287..7066
                     /locus_tag="J8V14_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HBA0922379.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7059..7376
                     /locus_tag="J8V14_002297"
     CDS             7059..7376
                     /locus_tag="J8V14_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBA0922380.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7620..9230
                     /locus_tag="J8V14_002298"
     CDS             7620..9230
                     /locus_tag="J8V14_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBA0922381.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9387..10235
                     /locus_tag="J8V14_002299"
     CDS             9387..10235
                     /locus_tag="J8V14_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBA0922382.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3230 a         1800 c         2071 g         3165 t
ORIGIN      
        1 aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc
       61 tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt
      121 tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc
      181 tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt
      241 gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt
      301 gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg
      361 atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa
      421 cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga
      481 tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg
      541 tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg attaactgct
      601 tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt
      661 tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc
      721 acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata
      781 cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag
      841 gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca
      901 taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg
      961 gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa gcaagtagcg
     1021 agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata
     1081 tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct
     1141 tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact
     1201 ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa
     1261 ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca
     1321 cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct
     1381 ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc
     1441 gcatgcttgt cgtcaactaa cttcccacca aattctaaat ataatttgtc atagtggttc
     1501 actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccaatt
     1561 tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt
     1621 caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta
     1681 gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca
     1741 gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat
     1801 gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct
     1861 aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa
     1921 aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg
     1981 aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt
     2041 ttataacaat tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg
     2101 agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg
     2161 aactagagga gacgaacgcg gatcaggata caggacaaga atcgtcttct ttttacgata
     2221 tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa
     2281 aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg
     2341 aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga
     2401 tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa
     2461 cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg
     2521 caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc
     2581 aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg
     2641 ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg
     2701 atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat
     2761 tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga
     2821 cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc
     2881 ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag
     2941 agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta
     3001 ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca
     3061 aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca
     3121 agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacatgaa
     3181 aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata
     3241 actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg
     3301 attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca
     3361 gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc
     3421 acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat
     3481 gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg
     3541 atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc
     3601 gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa
     3661 atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta
     3721 ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca
     3781 agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg
     3841 aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca
     3901 atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta
     3961 gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc
     4021 tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc
     4081 ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa
     4141 ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat
     4201 gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca
     4261 aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt
     4321 caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt actttactta
     4381 agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt
     4441 ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt
     4501 tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt
     4561 tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt
     4621 ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata
     4681 ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca
     4741 ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat
     4801 tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa
     4861 aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga
     4921 agtggtcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa
     4981 agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact
     5041 ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt
     5101 gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt
     5161 taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat
     5221 ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct
     5281 agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga
     5341 aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca
     5401 agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt
     5461 cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc
     5521 ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat
     5581 actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa
     5641 agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga
     5701 aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc
     5761 aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct
     5821 gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat
     5881 catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc
     5941 tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt
     6001 tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga
     6061 tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat acattgaaac
     6121 agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa
     6181 aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg aatttatgtt
     6241 acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga acaaaaagaa
     6301 aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa gctatatacc
     6361 agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc tcttatcagg
     6421 actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat ctatgcttac
     6481 gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat tacgctatac
     6541 attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt ttttggctgt
     6601 cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt tttcaactga
     6661 cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga ctgcatgggc
     6721 cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg ctgaccttgt
     6781 ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga aaagcgcact
     6841 actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg tattattcca
     6901 aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat ttatgctgga
     6961 gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag atgtcaatga
     7021 gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta tatttgctac
     7081 ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg atgctttatt
     7141 ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta ccggtggcat
     7201 tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg gatattgctt
     7261 ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa acacgttcga
     7321 tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg
     7381 ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc tatccgaact
     7441 gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa agattagtgt
     7501 tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg tccttctatg
     7561 attttttgct ataataggga agatgtttat tttatgaaag aatagttgag ggaatttaaa
     7621 tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt ctatgtaaag
     7681 ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat caagatggta
     7741 tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc ctgatgcttg
     7801 gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag
     7861 ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt gtcatcggta
     7921 tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc agtccgattg
     7981 ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg attgctatcc
     8041 caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa
     8101 cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt acttattact
     8161 tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca gtcgcttctt
     8221 tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga aaagacttct
     8281 tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac cgatctgcct
     8341 ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc attactatcg
     8401 ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt ccagagatcg
     8461 cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat aaattgacat
     8521 tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata ttaagcacga
     8581 tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt tcgattgcgt
     8641 tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca ctatacacat
     8701 tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg ctagcttctt
     8761 tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat caccatcgtt
     8821 ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa gtaatcggat
     8881 taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc ggtattgtct
     8941 ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta gcaaagatca
     9001 gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta tgttttgccg
     9061 ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca atgatttatt
     9121 gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc ggaaaaaatg
     9181 ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag gttaactaat
     9241 cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat
     9301 tttccaattt tagtttcata tctaatctta cttatgatat agttataagg tctggaaaaa
     9361 ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat agttcggcat
     9421 atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc cctttgatta
     9481 tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat tatggacttt
     9541 taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa gttttgggtt
     9601 tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat ctttcttcag
     9661 ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc ggagtaacgg
     9721 tcattcctta tgactctaag atcactagta tgccgatggg acacatggta gaagctgcct
     9781 tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc gatcgaatcc
     9841 gagataatac ctatgcttat ctgattgtag atgatttgaa caatttagta cgaggaggac
     9901 gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg atcctgacat
     9961 tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc
    10021 gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc aaactttacg
    10081 tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg cagaaaaaat
    10141 atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca catttaggtg
    10201 aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa aaacatacca
    10261 aaaata
//
LOCUS       DADQRX010000096         9917 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.099, whole genome shotgun sequence.
ACCESSION   DADQRX010000096 DADQRX010000000
VERSION     DADQRX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.099"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..620
                     /locus_tag="J8V14_002300"
     CDS             414..620
                     /locus_tag="J8V14_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBA0922383.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(822..1823)
                     /locus_tag="J8V14_002301"
     CDS             complement(822..1823)
                     /locus_tag="J8V14_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA0922384.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1828..3741)
                     /locus_tag="J8V14_002302"
     CDS             complement(1828..3741)
                     /locus_tag="J8V14_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBA0922385.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3909..4415)
                     /locus_tag="J8V14_002303"
     CDS             complement(3909..4415)
                     /locus_tag="J8V14_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA0922386.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4575..5015
                     /locus_tag="J8V14_002304"
     CDS             4575..5015
                     /locus_tag="J8V14_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBA0922387.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5041..6198)
                     /locus_tag="J8V14_002305"
     CDS             complement(5041..6198)
                     /locus_tag="J8V14_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922388.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6201..6557)
                     /locus_tag="J8V14_002306"
     CDS             complement(6201..6557)
                     /locus_tag="J8V14_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922389.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6855..7829)
                     /gene="sgrA"
                     /locus_tag="J8V14_002307"
     CDS             complement(6855..7829)
                     /gene="sgrA"
                     /locus_tag="J8V14_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBA0922390.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8025..8864)
                     /locus_tag="J8V14_002308"
     CDS             complement(8025..8864)
                     /locus_tag="J8V14_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBA0922391.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9049..>9917
                     /locus_tag="J8V14_002309"
     CDS             9049..>9917
                     /locus_tag="J8V14_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HBA0922392.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYV"
BASE COUNT         2978 a         1976 c         1490 g         3473 t
ORIGIN      
        1 ttatttttgt ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc
       61 ttgataacac tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt
      121 gatatgattg ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct
      181 tttgggggta tcctaaatct tttctattat attcccatat tttctattat tggtctagac
      241 gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc
      301 atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat
      361 ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag
      421 ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt
      481 gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac
      541 cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac
      601 gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt
      661 gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca
      721 agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg
      781 agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt
      841 actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg
      901 ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca
      961 aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag
     1021 aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct
     1081 gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta
     1141 gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata
     1201 aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct
     1261 tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca
     1321 atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct
     1381 tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata
     1441 tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt
     1501 ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt
     1561 tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt
     1621 tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact
     1681 tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg
     1741 atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc
     1801 actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat
     1861 aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc
     1921 agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg
     1981 aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc
     2041 tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa
     2101 aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa
     2161 ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag
     2221 ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt
     2281 tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg
     2341 gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc
     2401 tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc
     2461 atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt
     2521 atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc
     2581 atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc
     2641 tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc
     2701 aataattcct ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc
     2761 tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt
     2821 tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct
     2881 attaccagtc gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt
     2941 gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac
     3001 tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg
     3061 tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt
     3121 aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa
     3181 agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc
     3241 ataatcatta acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc
     3301 tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc
     3361 attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac
     3421 tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg
     3481 gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt
     3541 ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt
     3601 ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt
     3661 ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg
     3721 ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa
     3781 acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct
     3841 cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa
     3901 gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa
     3961 gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac
     4021 atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct
     4081 gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa
     4141 ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata
     4201 gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt
     4261 tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat
     4321 gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa
     4381 tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt
     4441 gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt
     4501 acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc
     4561 tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac
     4621 gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc
     4681 aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg
     4741 aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata gatgaactca
     4801 gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta
     4861 tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc
     4921 tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg
     4981 attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc
     5041 ttaattaacg aaatccaatt tttccaatat ggatctattt ttctcattcc tgatttttat
     5101 gatttcagct ttcagacgcg cccaatcatt ttttgtaggt tgtccattcc agtaaaatat
     5161 ctgtgtattc gtttgatcat atgccttttt gtcgatagga taatcgctaa tgacaatatc
     5221 aggtaactga tcagcggatg cactatattt tattgggcgt tcagtcatag ataatatcct
     5281 tttctttgcc cataattcct gtgttttacc atactttgat tgaatataga atatgacagg
     5341 ttgttcgtgt tttataaaaa tatccctgac taacatacag tattgaaaaa tcaaccgtct
     5401 gttcttttca taataagtat taagctctgg aattctgcga gctaccccac taaaaaactg
     5461 ttgcatgatt ggatacatca ctggaaatat atgctgaaaa tctctgtctt ctgctgattt
     5521 tccaaaagca tccaccttct tttttgtccc aaaaaccctt gaatacaaat ggatcattgc
     5581 caaattgaca tatagataat ttttttctga agttgtaaaa caaatcttga agtttttttc
     5641 aaaattttct acaatagacg attcaatcca gccccaactt tcttgtactt tatctggagt
     5701 atccaacaaa tcttcttcca aataggtgtt cattacacca aacataaaat aaaggtaagc
     5761 ttgctctgtt ggagtgatgt gattcatcga ccagaagttt gcttcaaaaa aaggctctag
     5821 tatgtaatgc gtaaactctt cttttgttat caatggatgc acaaatttgt tcatctcttc
     5881 tgggacatac gtaatcgagt ggccttgttt cattcgttgc aacgaaattc ttagtaggct
     5941 gatccatttc tttctatcta tactacgcgt atatggcgca tgtttcgcaa atttttctaa
     6001 tagttgacgt ttatttttta tacttgataa tatatcagaa tgattttcgt cgaatgactg
     6061 ccaatacatc aagtgaaaaa agtatcggat acggtattct tctccttcta tcggattacc
     6121 ttttttcaag ctgcaagata aatgatagtt tttcaaaata gtattaagtt ctcttaattt
     6181 tctttgaatc gtacgcaaac ttatataatt ttttgactaa gattttctaa ggattgataa
     6241 gtacctttgt aaagtgaatc cagtaaaata taaccaatcg ttccctctaa aagtatagaa
     6301 taaactttac tcagtaaaaa ctgagaagaa aactcgactt ttatcatctt gttttctgaa
     6361 agaaaagata atttatattc tggatacctt tcgcaaaacg attctgcttg ttgaatcacc
     6421 gaattcatct tagccagggt gtagtttaga tctaaagcga tatccttcac cacatataat
     6481 ttctcgtctc tttcataaca atagttcaaa aattgatata cttgcctttc attgatatca
     6541 ataaattctt caaacaaagt acatactcct ttttatctat tatttagact tattccgaca
     6601 gctacatgaa taacgtacaa atagattcgt tactgatttt tcagagatac taccatgcgc
     6661 agtgtgcatt gtcaattatt cttgctctta ttttccattt gtgttcctgt tatttcctgt
     6721 ttctcaaata tgttagtaaa caaaaataga tctggcaaag ctacaacttc gccaggcaat
     6781 acagcactct cgaatgaaat attcactcaa aaatctatcg ttttattcaa ttttagatct
     6841 gttgctttta ttctttattt cactcgatgg aagagagaac gaaaatagct tgctcctaca
     6901 atcaaaatga gtaacacacc catccatgta attagcttgt tcaaggttct actaccagtt
     6961 tctggtaaat cgctttttct attatctgtt tctttcgctt tttcttgatg atcaattcca
     7021 cgagaaacaa aatcaggctg ttttagcaca ctttttaggt tgctccctgc atttctcttt
     7081 tgttccttag ttggtatgat aggtaccaaa tcaagttcat tatttacttt tcctgtgctg
     7141 ctttcgctgc tcgtgcttcc tgtgctactc tcactactca tgcttcctgt gctactttca
     7201 ctactcgtac tctccgtact gctttcgctg ctcgtgcttc ctgtgctact ttcactactc
     7261 gtactctccg tactgctttc gctgctcgtg ttttctgtgc tactttcact gctcgtactc
     7321 tctgtactgc tttcaccgct cgtgttttct gtactgcttt cactacttgt gctatccgta
     7381 ctgcttggcg gtccaacaac tgtccaactg cttggtttca aattctccgt gatcgtcatc
     7441 tgtccatcat aaaaaacaat caaatcaact ttatctttac tcttgatctc atcaatcgat
     7501 agttctgcag aaatcattat tgtatactca ataccagcgg tccattgtac gtcttttaaa
     7561 taaacttccc caataacttt ttctgaactt ccattaccat cccaaccata tgttagaaca
     7621 gcatgtacat cactagcatt cacgcttttg tttggtttga ctggaatagt aatgacttgt
     7681 tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa atgaaagatt ctcatttgcc
     7741 cgttcatttg cagaagtact gattccaatc attgctgcaa ttatactcag cccaagaata
     7801 acaaataata cggtcgctgt tttcttcatt tctatcactc tccatataat taccttctat
     7861 ttgataaagg aattatataa aatgaaaatg atgagaacac gacagtcagt tgtgaaaatc
     7921 taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa tcaatgacag
     7981 acttaaaaaa gcaagaccga tatcagcctt gctttcttca tcttttattg ataaacagca
     8041 gtgtatttct tcatccgctt tgttttatat gcatagagta acaaaccaac aatcaagact
     8101 aacaagaata atgtagttga tatagatgct tcagccccaa agtttccccc agtaattatt
     8161 gtattttgcg attggatcag agtggagaaa aaaccttgtt cttgcgttcc gctgactggt
     8221 atacccaaaa tagctccttg tgtccaattc caagtaatat gagcaccaat agccgtaaat
     8281 aaacttcctg tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa gatattgaaa
     8341 actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa tgcagcagat
     8401 ccaatcattg ctattaacgg accaaatcta agcatctgat gctgaaaaaa gccacggaat
     8461 aaaagctctt ctgatgcccc cactgcaata aataaaataa aactcgttat cagcggcaca
     8521 atgtttgcgt ttcctgaatc taaccgatac gcccccgtca aatataatgg actagtaata
     8581 agcagaaaca ctccaattcc aacaacgaga cctcccgcta aataccccca gtcttttcct
     8641 cgaacagaaa caccataatc ttctattctt tttccttcga ccttactagc aagaaataaa
     8701 gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa ttgctccaat ccattcacta
     8761 tttccaataa acatatccgc aagtgcatta ttgacaagaa ttcctaaaac aaaaccaact
     8821 aagactatta aaacagatcc aatcaaccga ataatcctga tcatctttca tccctccttt
     8881 atattaagtt taacaaccct tttctttctt gtctcgtgaa aactatgtaa atgttctcca
     8941 tcatagagag aatagatttt tacgaactaa gaatatctac agcaacctta cgaaaatgaa
     9001 agactgtctc cctaattctt ctgttagact gtttttaata aggaaaggat gggaaatttt
     9061 ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg caattttact attctcaggt
     9121 tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg gaacacttac agttcaggat
     9181 ttttatgata acccaaaagt taaatcaaat aatcaaacac tattaaaaaa aatgattgtc
     9241 ttcaaagcat ttgatgatgt atacggaaaa gagatttctt caaaggaagt aacaaaagaa
     9301 tataatgaac aaatcaaaaa actcggtcca aactacaagg aacaattaaa agcagttggc
     9361 caaacagaag agacatataa gaaactcttt aaacagatgc tggctttcca atacggtttg
     9421 aaagccaatg taaaattaac agataaagat ctagacactg cttggaaaga attctatcca
     9481 gaagttagta cacagatcat tctattttcg actaaagaag aagcagataa agcaaaaaaa
     9541 gaagcaaatg aaggagaaaa tttctccaag cttgttcaag cttatggaaa aaataaaaca
     9601 ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca atccagagat tcctactgaa
     9661 gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt ctgatatcat tcctgtaaca
     9721 gatccaacga cttatcagca aagctattat ctagtaaaaa tggtaaaaaa acaggataaa
     9781 ggttctaata aagacaaata caaatctgag ctagaaaaaa ttgctaccga agcaaaaact
     9841 cttgatacgg aattcatgga taaaacaatc cgtaaagtga tgaaaaaaga caatgtaact
     9901 attaaagatc cttatgt
//
LOCUS       DADQRX010000097         9811 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.100, whole genome shotgun sequence.
ACCESSION   DADQRX010000097 DADQRX010000000
VERSION     DADQRX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.100"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..2154)
                     /locus_tag="J8V14_002310"
     CDS             complement(34..2154)
                     /locus_tag="J8V14_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBA0922393.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="J8V14_002311"
     CDS             2384..3562
                     /locus_tag="J8V14_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBA0922394.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="J8V14_002312"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="J8V14_002312"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBA0922395.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="J8V14_002313"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="J8V14_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBA0922396.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="J8V14_002314"
     CDS             complement(4915..6168)
                     /locus_tag="J8V14_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA0922397.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="J8V14_002315"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="J8V14_002315"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBA0922398.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="J8V14_002316"
     CDS             7746..9092
                     /locus_tag="J8V14_002316"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBA0922399.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="J8V14_002317"
     CDS             complement(9400..9786)
                     /locus_tag="J8V14_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBA0922400.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DADQRX010000098         9593 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.101, whole genome shotgun sequence.
ACCESSION   DADQRX010000098 DADQRX010000000
VERSION     DADQRX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.101"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..641)
                     /locus_tag="J8V14_002318"
     CDS             complement(<1..641)
                     /locus_tag="J8V14_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBA0922401.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="J8V14_002319"
     CDS             complement(643..1407)
                     /locus_tag="J8V14_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBA0922402.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="J8V14_002320"
     CDS             complement(1415..2092)
                     /locus_tag="J8V14_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBA0922403.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="J8V14_002321"
     CDS             complement(2129..3514)
                     /locus_tag="J8V14_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA0922404.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="J8V14_002322"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="J8V14_002322"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="J8V14_002323"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="J8V14_002323"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBA0922405.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="J8V14_002324"
     CDS             4453..5310
                     /locus_tag="J8V14_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBA0922406.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="J8V14_002325"
     CDS             complement(5391..5726)
                     /locus_tag="J8V14_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922407.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="J8V14_002326"
     CDS             complement(5793..7163)
                     /locus_tag="J8V14_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HBA0922408.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="J8V14_002327"
     CDS             complement(7241..7777)
                     /locus_tag="J8V14_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA0922409.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="J8V14_002328"
     CDS             8086..9567
                     /locus_tag="J8V14_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBA0922410.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DADQRX010000099         9515 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.102, whole genome shotgun sequence.
ACCESSION   DADQRX010000099 DADQRX010000000
VERSION     DADQRX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.102"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..324
                     /locus_tag="J8V14_002329"
     CDS             130..324
                     /locus_tag="J8V14_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922411.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            593..892
                     /locus_tag="J8V14_002330"
     CDS             593..892
                     /locus_tag="J8V14_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922412.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(989..1423)
                     /locus_tag="J8V14_002331"
     CDS             complement(989..1423)
                     /locus_tag="J8V14_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0922413.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1655..2365)
                     /locus_tag="J8V14_002332"
     CDS             complement(1655..2365)
                     /locus_tag="J8V14_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA0922414.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2355..3215)
                     /locus_tag="J8V14_002333"
     CDS             complement(2355..3215)
                     /locus_tag="J8V14_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBA0922415.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3220..3858)
                     /locus_tag="J8V14_002334"
     CDS             complement(3220..3858)
                     /locus_tag="J8V14_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA0922416.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3873..4172)
                     /locus_tag="J8V14_002335"
     CDS             complement(3873..4172)
                     /locus_tag="J8V14_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922417.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4215..5699)
                     /locus_tag="J8V14_002336"
     CDS             complement(4215..5699)
                     /locus_tag="J8V14_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA0922418.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5719..6180)
                     /locus_tag="J8V14_002337"
     CDS             complement(5719..6180)
                     /locus_tag="J8V14_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA0922419.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6198..7265)
                     /gene="ulaG"
                     /locus_tag="J8V14_002338"
     CDS             complement(6198..7265)
                     /gene="ulaG"
                     /locus_tag="J8V14_002338"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBA0922420.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7483..8253
                     /locus_tag="J8V14_002339"
     CDS             7483..8253
                     /locus_tag="J8V14_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA0922421.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8526..9353)
                     /locus_tag="J8V14_002340"
     CDS             complement(8526..9353)
                     /locus_tag="J8V14_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922422.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2963 a         1944 c         1490 g         3118 t
ORIGIN      
        1 tagttatatc gagtgaaaaa aactttcaaa ttgattcttt ttatttatca taaagatata
       61 gatacataat cattttcact tacatatatt tgaatattaa ctaaaaacag caagaaagaa
      121 gaggatcaaa tgtattatgt agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg
      181 aaaggtatca gtaacgaata tcctttactt ggttcatgga aagaagctgc tcctttttcc
      241 aaaccatgtg ccataaaaat aaagaacgag ctagaaaaag aactgacttg tggtaaagct
      301 gttgtagata tcatcgaaaa gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag
      361 cgacgtacaa attgctaaga ttatagaatt agaagaagaa gacgcagtgt tgacaattga
      421 aaaaaaataa atgtttgtat accatatgat tatttcaata tgtatcctgg aattggagat
      481 ctcgtagagc tatatcaaga tgaatcaaat atacttgtca ttcatcatga aatatagaga
      541 cttataaaat tgtttatttt tataatgtat cagtatagaa aggaaataaa acatggatta
      601 tactgttgaa tttgacagtg aactacttaa gcaatttcca caagaagaac atttcatgta
      661 taaagaagca atttcccaca aagtgacaga gtatttggag cataattttt ttcgtataaa
      721 gccagtgcgt actcgttcgg caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa
      781 aaattatcgc atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt atattagttc
      841 gaatttgcaa aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat agctgagcat
      901 tggaggatag attgagtgat actaagtcta tcctcttgtt tttctaaaga actaagctag
      961 aattttttct tttgtacgtt attatatgtt atttgtcatt accaaaaagc accttttttc
     1021 ttctagtatc tatgacataa gaaataccac ccatttcctt tacttgaaca aatcctactg
     1081 attcataaaa tttttttgtt ttctcagtat catctgtcaa taattcgatt tggactggtc
     1141 cataactagt gatcttctcc aaagcttcat tcaaaagcac tttgcctatt ccttggcgct
     1201 gatacctcgg atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc
     1261 aacgaacgat tccgataatc ttcccttccc cattacgctt tatcagagat tcagattgtt
     1321 caaatgctct agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat
     1381 ataattcaga aatctcatcg atcataaggg aatcaatatt catttttttc ttcctttctt
     1441 atacaattct ccctctaata atacagttct ttgactattt aatctatcac atataactct
     1501 cctgtaaaga ttctctcgat tccttaaaat tcctctttcc ttttagaatg catacttttt
     1561 gataaataaa aaggttgtga taaattttct tcacaacctc tgtttctctc tatcaagtgt
     1621 tatcttttaa ttttctaccc ttaccctagc atctctattg cccgtagtat gcatactttc
     1681 catgtttgcg aaaataatgt ttatctagta aatattgcgg gatttcacat atattggggt
     1741 tgatcagctc gttttctttg gccatcagac aaatttcatc taagatcaaa ctgttttcta
     1801 ctgctttcat aggtgaatcc ccccatgtga acggtccatg tccatagaca agtactccag
     1861 ggacttcgtt tggatctaat tttctttcct taaacgtctc gacaatcaca ttccctgtat
     1921 gaacttcgta agcttctctt acttcttctt tcgttaattg gcgtgtacaa ggaaccttac
     1981 cataaaaagc atcagcatga gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc
     2041 acatgacgga gtgcgtggaa tgagtatgag taatagcagt tatatcttca aaagtttgat
     2101 acaaaacaac atgtgtgggg agatctgaag atggttttaa gctatcttct tctattatat
     2161 ttcccgacaa atctgttacc accatttgat ctgcttgcat gcattcatat ctgacaccgc
     2221 ttggcttaat gacgatgact cctagctcac ggtccacttc actcacattg ccccaagtca
     2281 atttgactaa tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt tgcttcattg
     2341 ctgcaatttt ttcttcacaa gacatcatag ccagcctcct tcaatttaga acttaagtag
     2401 tcttttgcct gctggatttc tcttcgaaag tcttggcttt tttcactcca catttcaatc
     2461 agaaatgttc catcataatc tagacgtttc aaatgtctta ataatccaag aaaatcaaca
     2521 caaccgctac caaaaggaac atcacgaaac tggcctgaat attcttttgt tacagggtaa
     2581 gtatctttta agtggatagc agtgatataa tcgatccctt tctcaagttc tcgagccgga
     2641 ttattttccg gccatgcgga caaattgcct aggtctggat atacttgtag aaaaggtgaa
     2701 tgaatctgtt ctttgatttc taaaaatttt gaaatggagt tcataaatgg atcatccatg
     2761 atttccacgg aaagaatgat gccatattta gaagcttcct ttacgctttc ttttattcct
     2821 tcgataaagt cttctctgga attcattgat ttctcttcat aataaacatc gtatccagct
     2881 atttggatta ctttgacgct tagattatgc gccaaatgga ttgctttttt cattagttgt
     2941 ttcgctgctt ttttcttttc tggatcagca gaaccaaacg gaaatcgccg atgcgcactt
     3001 aaacaaatcg aatggatacg tacaccggag ctgaacatct cttctctcag ctcagctatc
     3061 ttttcttcag accaatctaa tcgtgctaac cgctcatccg tctcatcaat cgacatctcg
     3121 ataaaatcga agtccatttc ttttacaagg gaaaaacgtt ctttccatga aatatctttg
     3181 ggtaacgctt tttcataaat cccaatttga gccatttcct tacccccaga tacgaatgat
     3241 ctcatctttg aagttttgtg cggccttcat cggatcattt gctgctgtaa taccacgacc
     3301 agtgataaag gtatacacat cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc
     3361 accagttaca gaaacattaa agcctagttc aatcagtttt ttgatcttcg ataaatcttt
     3421 ttcaccccaa gtttcaccag ataacagcgc atcacggctt tgatggtaga ttacctgccg
     3481 gataccgatg ttatgccaat ccatggcttg ttcaaaagtc caattgccgt agagttctac
     3541 ttgcaattca cctatttctt tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat
     3601 gactgtcatc cagtctgctc ctgcttttgc aacattactc gccactgttc ctccagcgtc
     3661 tgcacatttc gtatcagcga ctagtttttt atctgggaac atactgcgaa tacagcgaat
     3721 cgctttttgt ccttcctgta agcaaagaat tgtgcccact tcaataatat ctactacatc
     3781 accaactttc ataacatctc ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg
     3841 caaattcggc ctgctcatct tagttccttc ctttacttgt ttttttggta ccactttgtt
     3901 tcagcaaatt tttcactgat ttctttgact gacatcacat ttttgatacc gataactgta
     3961 actccttttt tctctgcctg agtaaacata ttcaaaaaat tgactggcgt aaaaactaca
     4021 tcgtattggc cagctgaact ttttccctct gaaatggcac aatgatggat tttggtaatt
     4081 ggtacgttta gttctttcat tgctttttcc acacttctca tcatcatcaa gcttgttcct
     4141 gatccatttg cacacgatac taaaattctc atcttaatcc ctccattatg agtgttgtct
     4201 aatcagattt tcttttatac tctctgcttt tctgcatatg tgtcatagtc ttctacaatc
     4261 aaaaaataac catcaggatt tttccgatat tgtaactgcg gaatagccaa taaaccaatg
     4321 acaactacgg caattccagc ataacctagg actttcatga ttactgtaaa caatggccaa
     4381 gctgttgccc agtcgaacat cccgatataa ccaccgtatg ttgacatccc aatccatgta
     4441 gcgaataatg ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa caggcaagcg
     4501 gctttgaatc cacctcggtt attggcataa actgctataa cggcattatc aaagaacaac
     4561 ggaatgaaac cggctatgac gatcgttggt gatttcaata aaatcagtaa accaatcatc
     4621 aaaaactgtc ccaaagcacc aaacaagacc ccaatcgtta ccgcatttgg cgaaccaaag
     4681 ccaaaagtag cagctacgtc aattcctgga acagctcctg gtaaaatcgt gtttgagatt
     4741 ccttggaaag attgagttag ttcatcgacg aatgtccgaa cacctaattg caagatcgcc
     4801 agatataccg caaaatttaa agaagtcgtc ataatgtaga ataagaaact ctgcccttct
     4861 tgcatgaatt gcgcttgaat caaataatct ttgcctagta caatcagaat gatcccaaag
     4921 aaaaatagca tgagtaacga agtagcaacc atattctcat tgaaaatcga caagaatcct
     4981 ggtaatttga tatcttcaat ttttttgtct tgcttgattg atctattttt cttttcatcc
     5041 cgctttttca tccactcagc taattttgca aatataaaga ttccgaacat ttgctgatgt
     5101 gccactgcaa atccggcacc ttctgtcaat tcctgcgtga ttcctactgt taagttagaa
     5161 gctactgtcc aataacaacc taaaatcagt cccataaaaa tcaatgcttg tatcctacct
     5221 agatcaggaa agcaaaacag taaaatccaa aaagcggtag ctgcttgttg aacctgaacg
     5281 tttccggtag tgaaaacgga acgcagtttc gtatatttct gaaaacggac taataagata
     5341 tttaaaacaa atgcaatcag taataaaatc atcacatctc caaaggtacg gccaaatgtc
     5401 tccattaatc cagcatccac agcattttgt ccaaaataag gatctgtaac catggcatcc
     5461 aaattgaacc gttcctttag tccaaccaag attggccgaa aattattgac caaaccacca
     5521 gagccaacag tcaaaataaa gtaaccgacc gttgccttta agaatccagc gatgcattca
     5581 tgaagcgggc gtttcagcaa aatatagcct aataacacga taaaaccaat caggtatgct
     5641 ggctgcgtca agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc
     5701 ttttttctcc tctatcaatt aattatattc tgccatcaca acttggtaat catcgatgga
     5761 gttcgtagag atgagacgct caaccaatcc atctgtcatt aataattcgg ataatttttg
     5821 gatattttct agatgttctt ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt
     5881 tttctcctca ttctcccctt cactttcaaa acggattggc tgtttcaact tggtaaatga
     5941 aatagctgtt ccaaaaacac catcactttt ttctgaagaa tgcggcattg ctacttgagg
     6001 tgcgatcaca atataaggac cgtatttttc tacacaatgg atgatctcct ccacatacgt
     6061 ttcatcaata atttccttat cgatcaatac aagacagctt tcggcgatag cttcacgcca
     6121 atccgtgatt tctttatctg ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa
     6181 gaaactcccc ttctttctta taaaaacgac ggaaacggtg tatcattttc aatcgagaag
     6241 acatcttcaa atcctcgata aaaattaaac tcaagatcat ctttgtttgt cggatagatg
     6301 aatttcccgc ctacttgcca gatataaggt ttaaatccat acttcaggcg atcctttttg
     6361 aatttccaaa tttcagtgat ctcttttgga tcagccataa aattagacca aatatcgtag
     6421 tggacaggaa tcactacttt tgcattcaat gactccgcca tgcgcagcat atcaacagaa
     6481 gtaactttgt ctgttatccc tcgtgggttt tcaccatagg cccctaaaca gacatcgatt
     6541 ttgtgttcat taccatgttt ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa
     6601 agattccctc ctgatgtttc gaataaatag tttactgcaa tttgatccat ctcctgaggc
     6661 atttttcctt ttaatacctc ttctggatct tcacaagtga ccaacgctgt ccgatcaaac
     6721 gcttctaaag ctactacatt gatgtctttg attttgactg catcacctgg ctttactgta
     6781 atcgtttttt cttcaggaat tccccatttt tgccacgtgc gaaccacttc ttttggccca
     6841 atgaatgttg cttcaggaca attttgatga actgctgcgg ctgtatggat atctagatga
     6901 tcggaatgga tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa aggatcgatt
     6961 acgaaaggct gagtacgtaa attaggctgc atattttgaa cgccactcat acgcatcatc
     7021 tgatggcctt ttttcatcat gccattccca tgactacgtt ttcccgtccc acaccataga
     7081 tcacataaaa tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca gcccagccac
     7141 cacatagcaa aagttccagg atccactact tctgtttcga tttcttcatt caagtatgtg
     7201 ccccactcag gaaatgtttc tactaaccac ttttcttttg ttacttcatt gacagtttta
     7261 ctcatttgct tgctcccccc atatcgttgt tccaacttac aagtaaatat taatgtgtat
     7321 cagcatccat gaaaaccctt ttatatgacc gtttgttttt tatatgattt tctttcagta
     7381 atcaatgatt gtttcagtat aataaagaca gatatgaatg tttaaatgaa caactagtca
     7441 aataaatcca tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc
     7501 aatattgaaa aacgtcaaaa agaaattttg gaactgcttc agcagactga acaactatca
     7561 actttcgaat tagctgaatc ccttgatgta tctattagta cgatacgaag agatttaaat
     7621 ttattagaag aaaaaaacga gatcatacgc aattacggtt attgttctta taattacaaa
     7681 aaccagactg attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa
     7741 gctagtaaat acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg
     7801 aaaacactgg attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc
     7861 aattactctc cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt
     7921 ccaaaagaag cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac
     7981 atttgtatca ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta
     8041 aatgaatcaa cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc
     8101 gctgatcatc gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt
     8161 gattatttga tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg
     8221 gggataaaaa ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg
     8281 attatcaaaa aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt
     8341 gataaaggaa tcccaagatg tatccatcca atcgatacca tcataatcta ttgcttcttt
     8401 tataaccgtt atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac
     8461 aaaagtcaca cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa
     8521 ccactttatt tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc
     8581 gctatttttt ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg
     8641 caagaagcaa tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta
     8701 tactcaatac ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa
     8761 taggtggcga tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc
     8821 aatattttaa gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag
     8881 tagatcattg ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt
     8941 tccaacactt gattaaaata aaataatgct gcatctggtt ttttcgcata aagatgatca
     9001 agcagtcctt gataaaataa aatttgattt aaaagatcag aattacttac tttttcctga
     9061 tcgatcgttt gtaaaatttt ataagattct tggtattttg atacagaatt caatgattga
     9121 atctcattta tttttttcct gattttgtct tcttctgttt ctactaatat atcggagatc
     9181 tcgacattca gctttttgca aagcgcactt aagatattga tgcctggcga catattcttg
     9241 ttttcgatat tgctgacagt tgcttgttca caaatgcctt ctgctaattg tacttgtgtt
     9301 aacccctttt tctttcggta ttcttttagt aatgctccat ctactttaat caaaacaatt
     9361 tccttccctt taaaaaatta tttctccttt atatcataca caaaaaaaag ttctaaacaa
     9421 ccaaaaatgt ttagaacaga ataaaaattt taattttaaa ataatattca aattaaacaa
     9481 ctggcgtatc tgctccttga tttctttttg tgtta
//
LOCUS       DADQRX010000100         9472 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.103, whole genome shotgun sequence.
ACCESSION   DADQRX010000100 DADQRX010000000
VERSION     DADQRX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.103"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            949..1422
                     /locus_tag="J8V14_002341"
     CDS             949..1422
                     /locus_tag="J8V14_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBA0922423.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="J8V14_002342"
     CDS             1431..1658
                     /locus_tag="J8V14_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922424.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="J8V14_002343"
     CDS             2294..2665
                     /locus_tag="J8V14_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922425.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="J8V14_002344"
     CDS             complement(2921..3163)
                     /locus_tag="J8V14_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA0922426.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="J8V14_002345"
     CDS             complement(3195..4097)
                     /locus_tag="J8V14_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBA0922427.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="J8V14_002346"
     CDS             complement(4110..4298)
                     /locus_tag="J8V14_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBA0922428.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="J8V14_002347"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="J8V14_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBA0922429.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="J8V14_002348"
     CDS             complement(4913..5806)
                     /locus_tag="J8V14_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0922430.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="J8V14_002349"
     CDS             complement(5884..6822)
                     /locus_tag="J8V14_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBA0922431.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="J8V14_002350"
     CDS             complement(6856..7206)
                     /locus_tag="J8V14_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBA0922432.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="J8V14_002351"
     CDS             complement(7239..8141)
                     /locus_tag="J8V14_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBA0922433.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="J8V14_002352"
     CDS             complement(8134..8991)
                     /locus_tag="J8V14_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBA0922434.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..>9472
                     /locus_tag="J8V14_002353"
     CDS             9325..>9472
                     /locus_tag="J8V14_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBA0922435.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAK"
BASE COUNT         2689 a         1780 c         1563 g         3440 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa ag
//
LOCUS       DADQRX010000101         9432 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.104, whole genome shotgun sequence.
ACCESSION   DADQRX010000101 DADQRX010000000
VERSION     DADQRX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.104"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..680
                     /locus_tag="J8V14_002354"
     CDS             <1..680
                     /locus_tag="J8V14_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922436.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="J8V14_002355"
     CDS             800..1000
                     /locus_tag="J8V14_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA0922437.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="J8V14_002356"
     CDS             1076..1273
                     /locus_tag="J8V14_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922438.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="J8V14_002357"
     CDS             complement(1570..3117)
                     /locus_tag="J8V14_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA0922439.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="J8V14_002358"
     CDS             complement(3201..4004)
                     /locus_tag="J8V14_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBA0922440.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="J8V14_002359"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="J8V14_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBA0922441.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="J8V14_002360"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="J8V14_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBA0922442.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="J8V14_002361"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="J8V14_002361"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBA0922443.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="J8V14_002362"
     CDS             complement(6389..7318)
                     /locus_tag="J8V14_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA0922444.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="J8V14_002363"
     CDS             7513..8196
                     /locus_tag="J8V14_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBA0922445.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="J8V14_002364"
     CDS             8357..9400
                     /locus_tag="J8V14_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0922446.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DADQRX010000102         8850 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.106, whole genome shotgun sequence.
ACCESSION   DADQRX010000102 DADQRX010000000
VERSION     DADQRX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.106"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="J8V14_002365"
     CDS             complement(324..893)
                     /locus_tag="J8V14_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBA0922447.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="J8V14_002366"
     CDS             complement(967..2256)
                     /locus_tag="J8V14_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922448.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="J8V14_002367"
     CDS             2610..3458
                     /locus_tag="J8V14_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBA0922449.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="J8V14_002368"
     CDS             3472..4545
                     /locus_tag="J8V14_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBA0922450.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="J8V14_002369"
     CDS             4535..6706
                     /locus_tag="J8V14_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBA0922451.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8839)
                     /locus_tag="J8V14_002370"
     CDS             complement(6818..8839)
                     /locus_tag="J8V14_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBA0922452.1"
                     /translation="MANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPII
                     LFSPMMGMEFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIA
                     MGITVAYIYSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQ
                     KLAELLPESVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESA
                     VTGESRGIKKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLE
                     SLSDKVAKWLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVV
                     ARSTSIAAKNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNE
                     ILQYLGALEANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVN
                     EKEVERLGLNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKE
                     RKITPVMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGI
                     NDAPSLARASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWW
                     GAGYNILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         2603 a         1795 c         1595 g         2857 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt
//
LOCUS       DADQRX010000103         8819 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.107, whole genome shotgun sequence.
ACCESSION   DADQRX010000103 DADQRX010000000
VERSION     DADQRX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.107"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..122)
                     /locus_tag="J8V14_002371"
     CDS             complement(<1..122)
                     /locus_tag="J8V14_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBA0922453.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILS"
     gene            297..821
                     /locus_tag="J8V14_002372"
     CDS             297..821
                     /locus_tag="J8V14_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922454.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            837..2336
                     /locus_tag="J8V14_002373"
     CDS             837..2336
                     /locus_tag="J8V14_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBA0922455.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(2424..3026)
                     /locus_tag="J8V14_002374"
     CDS             complement(2424..3026)
                     /locus_tag="J8V14_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA0922456.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3130..4086
                     /locus_tag="J8V14_002375"
     CDS             3130..4086
                     /locus_tag="J8V14_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBA0922457.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(4391..5347)
                     /locus_tag="J8V14_002376"
     CDS             complement(4391..5347)
                     /locus_tag="J8V14_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0922458.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            5521..6081
                     /locus_tag="J8V14_002377"
     CDS             5521..6081
                     /locus_tag="J8V14_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922459.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            6074..6898
                     /locus_tag="J8V14_002378"
     CDS             6074..6898
                     /locus_tag="J8V14_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBA0922460.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            6908..7525
                     /locus_tag="J8V14_002379"
     CDS             6908..7525
                     /locus_tag="J8V14_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBA0922461.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            7543..8226
                     /locus_tag="J8V14_002380"
     CDS             7543..8226
                     /locus_tag="J8V14_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA0922462.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
BASE COUNT         2953 a         1583 c         1874 g         2409 t
ORIGIN      
        1 gaaagaataa aattcctgat caatatggca gaaacaatca aagaaacgaa tgtggagaaa
       61 caaacgacaa ggatgatcgt gttctggagc tttaatggtt ttttcttaaa aaaaggaaac
      121 ataggctacc tcgaaagaat ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa
      181 attgtcaacg ataaacacaa ccttcctata aaaaacacca ctgagtcggt aaaagaagct
      241 atacagaatt ttcaaacatt ggtatgatag acgtagaaat ggaaggaggc gtgtatatga
      301 tagggggaat cgcacttgag acgatttatt ggtatacatt gctgatttgt gcaggattgg
      361 cagtcctatt gattattttt ggagatgtat tcgattttga tggcccgctt gatccgatgc
      421 ttattatccc ttggttaact ttcacaagct tatttggttt tctaggtgag cgtttaacag
      481 atcaatcatc tttattttta tttattgcta gtggaggcat atcgatagtg ttggtatttt
      541 tgcttaattt ctatatattg atgccgatga aacatgccga atcaacgatg tcgtcttctg
      601 aaaaaactct ggaaggacaa gtagctgttg tgatcacacc gatccctgta cgaggtatgg
      661 gagaaattca gttgaaaagt gttaccggtt ccattagtcg tccagcttgt ttttacagcc
      721 cccaagaaca gacagctgag cggggaacga aagtattgat cattgagatc aaggaaagaa
      781 tctgttatgt catgccttat gaaagcatga taaaagctta aaggagcgtg tgttaaatgg
      841 aaataccagt tgtttttatc gcgatcgcaa tcgcagcttt gattttactg atgttgctga
      901 tcgtctttgt agcgaagtac cagacagcaa aaccggatga agcgttgatc atcagtggaa
      961 gttatttagg aagcaaaaat gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc
     1021 gtggaggtgg cgcattcgtc ttgccagttt ttcaacgttc gaatcggatc agtctattat
     1081 cgagtaaatt ggacgtctcc acccctgaag tgtatacgga acaaggcgta ccggtgatgt
     1141 gtgatggtac ttcgatcatc aaaatcggtt catctgtaga agagattgct acagcagcag
     1201 aacagttttt aggcaagaca agagaagaac tggaaaatga agcaagagaa gtattagaag
     1261 gacacttgcg ctcgattctt ggttctatga ctgtagaaga aatctatcaa aatcgcgata
     1321 agttcagtca aagcgtacaa gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca
     1381 tcgtttcttt cactatcaaa gaggtcagag acaaaaacgg ttatcttgac tctttaggga
     1441 aaccaagaat cgcccaagtc aaaagagatg cagatatagc agaagcggaa gctctaaaag
     1501 aaacgcgtat caaaaaagct caagcagaaa aagaatcaca agcagctgaa ttacagcgac
     1561 aaacagagat cgcagaatca ttaaaagaaa aagaactgaa attagcaacc tacaaacaag
     1621 aacaagatgt tgctaaagca aaagcagacc aagcctacaa tttagaaagt gcccgagcac
     1681 aacaacaagt aatcgaacag gaaatgcaga ttaaaatcgt agaacgtcaa aaacaaatcg
     1741 aactggaaga aaaagaaatc acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa
     1801 aagcagatgc cgatcggtac gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag
     1861 tgactgaagc cgaagcagaa cgattccgtg tggaagcact ggcagaagcg gaagccaata
     1921 agacacgttt agctggacaa gcagaagccg aagcagcact agcaaaagga aaagcagaag
     1981 ccgaagcaaa acaaaagatc gccaatgcct tcaaggaata tggcgaagca gctgtcttga
     2041 gtatggttat cgatatgcta ccgcaattga tgcgcgaagc agcacagccg ctaggaaata
     2101 tcgaaaaaat ctctgttgtc gatacaggaa gcagttcagg agaaaccagt ggagcgaacc
     2161 gagtgacaaa ttatgccaca aatttgcttt cttctactca agaaacacta aaagcgacaa
     2221 caggacttga cttgaaggaa atcgtagaga atttgtcgac acgtgggact actagccaag
     2281 taaatatgta tgaagcgcca aaagaggcag aacaaaactc gatgaaggaa gaataaagta
     2341 gtacacttca ccgttcaatc aaaaatggag gaagactttt taaagttctt ctctccattt
     2401 ttttatttta ttgcaaaaga aaattagtcc tcgctatttt tcttttgttt ttggatctta
     2461 gcttctatct cttgtaaatt tgttcttagc aatcgttctt tcttttggag ttcgaccaaa
     2521 tcttctgata gttcttcctt cttttctgtt tcaggaggac gaagaaagaa attgataccg
     2581 attgcaatga atgtccctat aaaagtatct agtacacgtt ggatagcata caaagcggat
     2641 tcgccttgag ggatgctcaa cgcaatcagc aacaacgtag ctattgcgga aatgataccg
     2701 gaattattgt taataccatc tgacacgacg atcacaataa taactagaat aggaagcaag
     2761 atcaattcta ccaaaaaatc attgtgaaaa tagcttttga caaaaaaata aatgatagct
     2821 aatgtaccac ctaatgtatt gccgatgatc ctagactttc caaaagaaac ggttgtagtc
     2881 aagtcctgac gcaaagaaaa aacagcagat aatgcagcaa tcatcgggat tcctcgttga
     2941 gtcaaatgaa aaaacaagat acataaaaaa acagctaagg cacttttgat cgtacgcata
     3001 cctagtcgaa aacggccaaa ttgcatagag attctccttt ttggtctttt tactatcata
     3061 cctaaacttt tgtctgatag ctagtttttc attattcaag tataattaaa ataaaaaagg
     3121 gggaagtaaa tgcgtatagg aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg
     3181 atggtcacag cgggtagctt atcgctgttg tattggccag ctgcagggat cattgcatta
     3241 ttaagtgtag gaaataccaa gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc
     3301 tttatattag ctacggcaac agcactgttc agtttttttg ctgttgggta tactattttt
     3361 ggcttcagtc tttttttatt cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa
     3421 gggatagtag taaattcggt tttggtcact cattatttag tggaggaaaa catgtcttgg
     3481 cagctgatcg gcaatgaagc gggattaatg gcgattggat tagtatttgc tttgcttgca
     3541 aatgtttaca tgccagatcg tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa
     3601 gaatttcgaa atattttgag acaaatggca gaatttcttt tatcagaaaa taaagaagat
     3661 gtgcagatca aatgcgaaca tttactgaca ttcattcgcg aaagccaaga agatgccaga
     3721 gaacatcaag aaaactactg gctcagacag ccgctatact acgaaactta cttctctatg
     3781 agaagagcac aggccaatgt gattaaagac atgctggaaa atctagagag aatccagcaa
     3841 ccagcttatt atggaaaaca tatctatggt ttattgattt atactgcaga aacattttca
     3901 gagtcaaatg atggcagaca aattttaaca cgtattgaag aagtttatgt attgtatcgg
     3961 caaatgcctc tgccgactag tcgccctgaa ttcgaggatc gagcagaatt gttccagttt
     4021 ttgcagtctt ttaaaagttt tattgaaatc aaggcagaat tctcccaaca aaaaatccaa
     4081 aaatagaggt tgtgaattca gctaatattc aggtcaaaaa gctgatttca caacctcttc
     4141 tttaaacggt gttctcctgt caactcctcg gtcacaagtc acaatattca acaaacatga
     4201 gaagctgttt tttgaaattg ctccttgtga ctcggagctg aacggtaggc tcacaacctc
     4261 tttcttaaac ggtgttcaaa agctttcctc tgcggtaata agtccagcca aacgaaaaat
     4321 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
     4381 ccttttaaaa ttacatattt ttccatagaa cttgcatttg atccaagatt tcgatgacat
     4441 cttttgtcaa gaagagagag cgattgggca agtcaccttc aatcatgtgt accatattct
     4501 ttatttcgta attcattgct tcagatgtat tcccgctttc gatgaattcc tttgtaccat
     4561 ctgtaaataa gatctccgca cgatcagcac gcgggtaatc agcaatcgtc acataaccgt
     4621 tctcaaaggc aacgataccg acttttggca tttttgcctg aaaagtcaag ctgacagcag
     4681 caagttcatt ttctttgtta cgcaagatag tgacggattg ttcgtccacg cctgtttcaa
     4741 aaggaaccat tgtagaggca actacttcag gttgagagct caaaaagaaa cgagcgaaag
     4801 atacagcata cgtaccgata tccaacagag ctcctccagc taattctgga ttgaagaacc
     4861 ggtttttagg gtctggttct ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc
     4921 ccagttttcc tgtatccatt attgagcgta actgctgata aagtggcatg ttgaagattg
     4981 tcatcgcttc tgctaagatc aaattctttt cttctgctaa gcgcatagcg tcagccagtt
     5041 cttttttatt catcgtgatg gctttttcac atagaacgtg tttgcctgct tccaatgctg
     5101 ccaagatatg gtcgatatgc tgtctatttg gtacagcaat gtacacgatg tcgatttcag
     5161 catcgtctaa catttcttga tagcttccgt aagcttttgg aatattgtaa cggtgagcga
     5221 agttttccgc tttttctgaa gttctggaag caactgctgt tagttcgctc gtctcttgat
     5281 tgaagctttc ggcaaactca tgtgcgatgg taccaagacc aacgattccc cattgatatt
     5341 ttttcatctg aaaacgctcc ttcatagttg gattgagttt cttgtctgtc tattattata
     5401 acaaaaatca cgaagaataa agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc
     5461 gatttaagag gtgctttttg atcaaaactt aagtagaata gttaaaagga ggggataaac
     5521 gtgcatcaag tatatattga tcttattatg agtgtattat tagaacagtt tgaaactgaa
     5581 aaaaattttt tagatgaata cttgctgatc agtccagaac aatggcaaaa atggaaagat
     5641 ggcgagaaaa atctgccatc tgaagcgatg cagaaagtaa aaaatctttt cactgattat
     5701 gaatggatgc taacgcaaaa gattttgcgt caaacgatta tttttcccga aaaacgaaac
     5761 gttgccgttg cagagtacaa gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc
     5821 aaagcagcaa aagtcgagct gatccctttg aaaaataatc agcaggctgg tagatacctt
     5881 gacttgaaag taagtattca atatgatgaa tggggctatg atgatatttt gaattttcgt
     5941 ctgccagcag atgttcaaaa acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg
     6001 aatgaaaatc tggaagaaac ttacgttcat gaggcgaatg gggaacgtga agaacaacga
     6061 aaggattttt caagtgaata aatttttagg atttggagca gttgtgatta tgatcgcagc
     6121 aatgggctat gttattttta gcgatgatga ttcgcatgtt caagccggca atcaagcagt
     6181 cgaacgaaca agcaccaaaa caactgctag taaagaaaag aacagtcaaa caaccagcag
     6241 caaggtaaag gacaaagaaa caaaagatcg tacgtccaaa caattgccag atgttaaaac
     6301 agacgattgg aagctagttt tggttggtcc agatcacaaa ttagaaaaag aaattgatga
     6361 gtctactcaa ttggcatcat taagtaacgg atacttgatc gacaaacgca ttgctccaga
     6421 atatgaagca tttgccaagg ctgctgaagc agctggattt ccattagtga tggtctcagc
     6481 ttaccggtct gtatcatccc aacaacaagt tttcgaacaa aatgtccaag atgtgatgag
     6541 tcgtcaaggt gtatcagaag aggaggcgac aaagattact aaagaaacga ttacggagcc
     6601 aggatacagc gaacaccaca caggtctggc agtcgatgtc gtcgatcaaa actggtacaa
     6661 cagttatcct tctactgtat tggatgcaag ttacggagat cagcctggag ctaaatggat
     6721 cgcagataat gctgcgcagt atggatttat cgttcgttat ccaaaaggac acgaagatat
     6781 aactaagatc acatatgaac cttggcactt acgttatgtc ggtaaagaaa acgctgagta
     6841 tatcaccaaa caccaattga cgctagaaga atatctgaat gaattaaatg aaaagtagga
     6901 attgatcatg gcgaaaaaaa agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc
     6961 agtggttgcc ggtgtagcag tgatactctt agcctttgtt ttttccttga agaatctgtc
     7021 ttctcctttt actgataatc agcaagaaaa tgaagcgcaa tcccatcagc aatttattga
     7081 acggcttgtt ccgcatgccc aagaactaca agatggttat ggagtgcttc ctagtatcat
     7141 tttaggacaa gctatattag aatcaaattg gggaaaaagt accttggcta gcaaatacaa
     7201 taatcttttc gggatcaagg cttatgggga tcagaagaaa gtaagcctgg aaacaaagga
     7261 atttgttaac gaagaatgga taactatcca aggagacttc aaagtgtatg attcatggga
     7321 acagtccatg gatgaccata cacaactgtt cgtccaagga gtagattgga atcctgcgct
     7381 gtatgaaaaa gtgattacgg ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc
     7441 aggttatgcg acagaccccg gttatgcgca aaaaatcatc caggtcatcg agacgtatca
     7501 attgaatcag tatgaccgga gatagcaaag gagaagtggc tgatggcaga acaatttacg
     7561 ccagaaatca tgacggaatt gagaaaagat atcgtaagag taccggaaat cattttagaa
     7621 gcaagcggga tcaagatatt tggaaaattg atccgttcga tcatctttac tacggatatt
     7681 gcgatcatac gtaatacaaa tgcgaatgca gtaattgctg tgtatccttt tacacctcat
     7741 ccagcgatta ctaaaagcat tattgaggca gctgacattc cagttttctc cggtgtaggt
     7801 ggtggtttga cacaaggtgc tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt
     7861 tcgttagggg tcgttttgaa cggtcctacg cctttagaga cagtaaaaga agtttgtcga
     7921 gtagttgata ttccagttat cagtacggta acgtcgaaat atacagaaat agatgaaaag
     7981 ttagcagctg gtatttcgat tatcaatatc agtgcaggta aagaaactgc agaagcagta
     8041 cgccattttc gtaaaaaata tcctgaactt cctatcattg cgacaggtgg tccaacggaa
     8101 gaaagtattg ctgagaccat tcaagcagga gcaaatgcca tcacatatac gccaccaagt
     8161 agcggagagc tttttagtcg taaaatggaa aaatatcggg aaactgagaa aaagaatcat
     8221 gaataacagg tgagaatcaa aagacgagga agagcagata gtggctcttc ctcgtctttt
     8281 gtactccagt actatttgtt cagaattcaa aaaaaagaga aatttttttg tttttttacc
     8341 tgtaatgtac ataagaagag ttaaaacctt ttgttattat tagcattcta acaaaaaaat
     8401 gtcttgattt attgaagcgc tttctataga atgaaaaacg aataaaaact aaaaaaggca
     8461 cccgacaatt tagtagcagc taaattgtcg gggcttgaaa agtcagcggg aactgaccaa
     8521 tcaagttgcc atagtcaatg cttgtgcatc gacgtgtata ttgtacctaa tttcatacag
     8581 aattactagt tatttttctg ttttttaggg aagagcgcac acgaagaagc atgcttattt
     8641 agctatgtca aggatattgg gcagttgtct gatatccttt tttctttgcg taattatcct
     8701 cccatcattg aagcaagaag tgtttttagc gatgaaagaa gaatcagcac tatctaattt
     8761 ttcttgaaag tattgagttg ttcatcctag gcagaacgat aaggcaagtc ttggttttt
//
LOCUS       DADQRX010000104         8677 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.108, whole genome shotgun sequence.
ACCESSION   DADQRX010000104 DADQRX010000000
VERSION     DADQRX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.108"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..402
                     /locus_tag="J8V14_002381"
                     /pseudo
     CDS             202..402
                     /locus_tag="J8V14_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1114..1719)
                     /locus_tag="J8V14_002382"
     CDS             complement(1114..1719)
                     /locus_tag="J8V14_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922463.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1901..2236
                     /locus_tag="J8V14_002383"
                     /pseudo
     CDS             1901..2236
                     /locus_tag="J8V14_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2401..2628
                     /locus_tag="J8V14_002384"
                     /pseudo
     CDS             2401..2628
                     /locus_tag="J8V14_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2754..3725
                     /locus_tag="J8V14_002385"
     CDS             2754..3725
                     /locus_tag="J8V14_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0922464.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3865..5178
                     /locus_tag="J8V14_002386"
     CDS             3865..5178
                     /locus_tag="J8V14_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA0922465.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5221..6087
                     /locus_tag="J8V14_002387"
     CDS             5221..6087
                     /locus_tag="J8V14_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0922466.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6084..6932
                     /locus_tag="J8V14_002388"
     CDS             6084..6932
                     /locus_tag="J8V14_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA0922467.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6946..8418
                     /locus_tag="J8V14_002389"
     CDS             6946..8418
                     /locus_tag="J8V14_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBA0922468.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8435..>8677
                     /locus_tag="J8V14_002390"
     CDS             8435..>8677
                     /locus_tag="J8V14_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0922469.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2735 a         1489 c         1784 g         2669 t
ORIGIN      
        1 gaacaccacg catagttttt attttgagat attctagaat gaaacacatt aagaacgaaa
       61 gcaaagcaaa agacgccaga acttctacta ccttctaagt tttttcatat ttgtttgtta
      121 tgctatacat agatctgcat aattagactt gcgctgcccg ttattgaagt ttcaggcagc
      181 gctctttttg ggggatcgat aatggaagaa gaaaaaatat ttgagaaacg ctggcagcta
      241 gcatcaagtg aacaaagagc aagatacaat aatttgatca cctcctaccc cactgttgac
      301 tagacatata aggaaaagaa atacttactt tggctgtgcc agttagatat agataccttt
      361 gaaacatttg aagtgatcct agataaaatt aaacagaatt gatcagaaac aagcgaagcc
      421 ttctctcggt tcgcttgttt tgctatttat tagttggctc agggcaactc taagctcgtt
      481 tgaaagaaaa ccgatggata atccccttgt ttgtaccact ttagaataca tggatgtacg
      541 tattgccatt gactcctatt agttactgaa aaaaaattta aacaagttga gtattgcaga
      601 taaatagctc cagtgaggtg aataactgct ccactcaagg caatagatag ctccatccag
      661 ggcaacaaac tgctccactt tcatatttaa tagatgaata accttctgta taatagaaag
      721 gcataccttt cggtatgaca acttatacag aatgttattt tattggttca ctatcgaaaa
      781 attttggagc tttatttttc cggtatttct caacgaacga ttagtttaac tgtgggaagc
      841 tccagacata cgattcgaga agttacttct cgagcagaca ggaagggggt gactgaacta
      901 accgctgata tgagcgatac ttggctgtag gaatatcttt ttccggaaaa acaaacaaca
      961 gcacgaggtt accttcctgt agactgggaa tatgttcata aagaactaat gaaaaagaat
     1021 atgacattga aactccttca tagagaatat ggggtacatg gaacatagaa acgaaaatgc
     1081 acggtagaaa agcgaatgaa tatagtataa attctacaaa tcattcgctt tctttatttt
     1141 atatatggtt ggtcgagaga gaccattttc tttggcaatt tctgcaactg gaactccttg
     1201 atttaacatg tccaccacag ttttaaagac taatcgtttt tgcggatctg ttgagttctc
     1261 agaatattca attggtcgcc ccttatatcg accagctttt ttggccaata atactccttg
     1321 ccgctgacgt tcacggattt tctcacgttc gttttgtgca atataactta gcaatgataa
     1381 aaacatatcg aacatagcag tatctaggag ttcatttcca gtattaaaat ctaaaaactg
     1441 tgcatctagt atttttaatg ctactttttt ctgtttcata aaggtaactg tattttttat
     1501 gtcttcataa tctctgccta ggcgatccag tgatgcaaca atgacacaat caccttctcg
     1561 gatataattt agtaattttt gaaactcagg tcgctggata tctttaccac tggctttttc
     1621 ttggaataat ttatcaatgt taaggtcttc catcattgcc aattgtcgat ctaaattttg
     1681 atcttgtgaa gacactctaa tatatccaat ttttgccata aaaactcctt ttaaaaaatg
     1741 atataattta catgtaaaat ttacttgtag ttttactgtt atcatagcat ttttaaaaat
     1801 aaattttaag tctacttata atttactttg gaggttttga atgaatgaaa ttaagcgcaa
     1861 taaaaattct tttggtgttt atttaggaga gcctagctcg gaacagttaa ataaaggtga
     1921 agctcgtcat gctctatgtc gaaatatatt ttatggtaga aaaggcaaac tttatcaaac
     1981 ttattacgac ggtatggagg aacagctcaa cgcattagga ttagtaacta atgctatcat
     2041 atattggaat tcactatatt tagaaaaagt ctttgaacaa attaaaacgg aagggttcga
     2101 ctgttccgaa gaagtgttac gcaagcaatt tccattaatt agtgatcaca ttaattttgt
     2161 tggcaaatat agtttccgct atgaaaaaga attatcgaat ggaatactac gtccgttaaa
     2221 tatagagaat tattgagaat gttcgtcttt tggttcctac cgtgcatttt cgcacggatg
     2281 ttccagatac tatttttgaa tgtataaaat tgaaaacaca agaatactaa taaatcaacg
     2341 attcttgtgt ttttaatttt tcactaccgt gcattttcgt ttctatgttc catgtacccc
     2401 aatcttgaaa aattaattaa agacttagat aaagccattg acgacttagt cgtggtgcta
     2461 ccggagtatc aatgtttaac cagtatccca ggtattggtc ctgtttaggc tgctggaata
     2521 ctcgctgaaa ttggtccaat tgaacgtttt tctgatgaaa taaaattgac aaagtatgcc
     2581 ggcctatatt ggcgacagag tcaatccggt aaatctgaat acgaaaattt ttttacgaaa
     2641 agcttcacgt ctaatatcta ttttaaaata aaaatattcc tgctaatttg gaatcgtttc
     2701 cttcttttgg tataataata cctgaaattc ttttgagagg agttgttcgt gaagtgaaaa
     2761 ctactatgaa ggatgttgct gcgttagcag gagttggtgt gggtacggta tctagagtaa
     2821 taaatggagt aaaggtgaaa gaggttacca aagaaaaagt aatggctgca attagtgagc
     2881 ttaattatga gcctgatgaa tatgccagag gtatgaaaac gaatagtagc aatacaatag
     2941 ctcttatttt acctacgatt tggcatccct tcttttccga gtttgcctac tatgtagagg
     3001 aagctctcag ccaaaaaaat tataagcttt ttttgtgtaa tgcgaacggc agtgcggaaa
     3061 aagaaaaaga atatatccaa atggtcaagc agagtaaagt agacggaatt ataggtatta
     3121 cctattcaga cattgataaa tatgtatcat caaatttacc atttgtcagt atagaccgcc
     3181 atttttcgga acaggtttct tatgtaacgg cagataatta tttaggtggg caaattgccg
     3241 ctcaagaact acttactcgt aactgtaaaa acttgtgcta tgttgggggt gtttcacctt
     3301 accctaacga aacaaacaat cgtaaagctg gtttttacaa tatttgtgag gaaaataaaa
     3361 tagctgtgaa ggtattggat atgcctgaac caattagcaa tttagaggaa caattgctcg
     3421 cttttttttc ggataatcct gaaattgata gcatttttac catcaatgat ttcatggctt
     3481 tggatgtgat aaaagcccaa aaaaaactag ggaaggaagc acctaaggat tttcaaatta
     3541 ttggttttga tggagtcaag ctatcgttgc aaaaagacta tttactatca acgattgtac
     3601 agccgataga ggaaatggcc aaggtttcag tagaaacact catggcgatg attttaaatg
     3661 aagaaccgcc taagcgtgtg gtagtaccgg ttttctttca cgctggagag acgactaaag
     3721 agtaaaaaac cggtttataa cgattttttt gagataggtt gacagaaagt gatttcaatg
     3781 atatcttaaa accaattaat tggaaacgtt accaattaat tgaaaaataa aacggaaacg
     3841 tttcaaaaaa taggaggaac atcgatgaaa aagagaatgt tagcaattgc agcaatttta
     3901 gcattaacag ctggaggact tgcaggatgt ggcagtaagg agaaaagttc aggtagcagt
     3961 gatccaggag aaattaagat ttgggttcag ttttctgatg aaacgcctga aggaaaagct
     4021 tgggcagaga ttgtgaacaa ttttaatgat gaatacgagg gtgaatataa agctgttact
     4081 gagtatattc ctcgtagcgg aagcggcggt ggatatgagg ataaagtaaa tgctgcaatt
     4141 acaaccaata cccttcctga tgttattacg ttagatggac cgaatactgc agcttatgct
     4201 cattcaggag tcattgctcc attagatgat tatttgaaag atgcggacat ggatgatgta
     4261 ttagacagca ttattcagca aggaacctac gatggcaaaa tgtatgcgtt tggtttctca
     4321 gagtccagtg tgggtgtcta ttacaataag tcgctgttca aagatgctgg aattacagaa
     4381 gatcaattgc cgacgttaga agatccttgg acttggacgg aatttcaagc aatttgccaa
     4441 aagctgactg ataaatacaa aacgcctgca attgatatgc aaatggcgtc gggagacgaa
     4501 atgctgacct acgcatatac accatttata tggtcgaatg gcggcgatgt cgttaatgct
     4561 gatggaacca aagccgaaga ctattttaac ggtaaaaaat ccgttgaagc gttggaattt
     4621 attcaagaat tggttgaaaa aggttacacc acaatcagtc ctattgaaaa aggatttgaa
     4681 actggcaaat atgccatgat gctgagcggt tcttggacaa tggcagattt agaggaaaac
     4741 tatcaagaga ttgattatgg tattttgcct taccccgttt cagatgcaac tggggagtta
     4801 gtttcaccaa ccggaagctg gcagttagca atgactgaga aaacagagaa aaaggatgct
     4861 tctgcggcct ttattaagtt tgcaaccagt acagaatcta gtcgtattat gagtttaggg
     4921 aacagtgttc tacctatcag aaattcaaca gttgatttaa ttaaagatga agtgtcggaa
     4981 ggaatgcagg tattaattaa gcaaaaccaa ctttctggac atgcccgccc agtggttgta
     5041 gcttatccgc aagtatctag agcattccaa caagcagttc aagatattag ctacttcgag
     5101 gaaaacccag atgtacaaaa agtagcagat agtcgcgctg ctgaaatgca acaggcaatt
     5161 gatacttcac taaaatagaa gtttctaaag ggggccaatc aagtgttaaa aaaaccgaca
     5221 atgaaagaca atttgatagg ttacggcttt ctagcgccag ctttgctgct gctgacgatt
     5281 tttctgatca tacccgtaat catggtaatt tattatgcct ttactgatta ttatttgtta
     5341 acgccagatg ctagacaatt tatcggctta gaaaatttta agcgactgtt cgctgatcca
     5401 atttttgtaa aaagtatttg gaacacggct aaatttgttg tttggattat tccggtccaa
     5461 ataggggcag ctttaggaat ggcacttatt atcaacaaaa atcgtaaagg aaatatcttt
     5521 tttaaagtag ccttttttgc gccagtcgtg atgtccttag tcgttatttc gattttatgg
     5581 ttgtacttgt taaatcctaa tgaaggtctg gttaatacgc tatttgcaaa gattggaatt
     5641 gcttcgcagc cttttttgac tagtcccaaa caagcaatgt atacgcttgt tttcgtatcg
     5701 gcatggcaag gtgcaggata tcagatgctg ctgtttttag ggggaatgca aaatattccg
     5761 caagacgttt atgaggcagc cgaaattgat ggtttttcta agtttcagca atttcgctat
     5821 atcaccatgc cattattaaa gcccacggcg atttttattt tacttaccac attgatttcg
     5881 gcctttaaac taatcattca gccaatggta atgactcaag gtggaccaat gaattctact
     5941 atgacaatgg tttattacat ttaccaaact ggatttacag accgtatggt aggctactcc
     6001 agctccattg cgttaatctt taccactatg attggtttaa ttacaattgc tcaaagaaaa
     6061 ttggtaaagg aggatgatcc agcatgacca agaaatttgg accgctaatg atcgtagaat
     6121 attgtttgct gttcctttta gcggcattat tcattttccc gatgctgtgg atggtagttt
     6181 cctctatgaa gcctgaagca gatgtctatc aaaatttagc ttccttcaaa gcttttttac
     6241 ctagtttgaa tccagctaat tggtttaaaa cttatcaaga ggttatttcc cggttcagtg
     6301 tgggaactta tttattaaac agtatttttt atggtttaac ctttgcctgt gggtcaatca
     6361 ttgtaaactc tttggcgggt tttgcctttg cgaaggttaa tttttcaggc aagaaaatta
     6421 tctttggagc tttgttagct ctgctgattg tcccgatgga gacggtttta attccgcaat
     6481 ttaccatcgt taattcacta ggcttagtga atactcgctt ggcagtgatt ttaccagcga
     6541 tggccagcgc gtttaatatc tatctgttta gaaatttctt tatcgcgatt ccagaagaaa
     6601 tcattgaatc agcgaaatta gatggtgcaa atattgttaa aattttcttt cacatcatgc
     6661 tgccaatgtc taaaccagcc atagccacag taggtgtctt atcctttatc tccagctgga
     6721 atgattatat ttggccgtta atggtcttaa ctgacagcag caaattttca atgcaagtgg
     6781 caattactac gatcaatact acacagccag tttatatcaa tcaagtgatg gctgttttaa
     6841 ccattgcaac gattccactg attattgttt atatcgttgc acaaaaatat attctgcagg
     6901 gacttggcgg ttctggaacg ggtataaaat aggagtgaat agtgaatgtt gcaaaaactt
     6961 ttaaaggcca atgagtttat tgagaaaaat gctcaaacag tcaatcaaga ataccggaat
     7021 aaatttcatt tgatggctcc gattggctgg atgaatgatc caaatggttt tgtttattat
     7081 caagaggagt atcacttgtt ttatcaatat tatccttacg acagcatctg gggaccaatg
     7141 cattggggcc atgctaaaag caaggatcta attcactggg agcatttgcc agtagcactg
     7201 gcaccaggtg aaagctatga caaggaaggc tgtttttccg gcagtgccat agaaaaggat
     7261 ggcaagctgt atttgattta tacaggacac aatgttgtgg aagggcaagt tcgacaagtt
     7321 cagtgtttgg cagtttcaga agatggcatt cactttgaaa aatatgccgg aaatccaatc
     7381 atcgcagaag atcacttagc ttctgttgca acgaccgaag atttccgcga tcccaaagtg
     7441 tttcagcgaa aagaagacta ttttactgta gtcgctacta aaactgcaga tgctcgtgga
     7501 caaattgtga tgttccattc caaagattta ctaacttggg agttttattc cgtattgctg
     7561 gagggaactc cagaacaagg cattgtatgg gaatgcccag acttgtttga actagatggc
     7621 aaagaagtct tgattctttc tcctattcaa atgactccac aaggcaatgc ttaccaaaat
     7681 acgaactcaa ctgttgcttt catcggtact gtcgattggc aaacaggcaa atttctagta
     7741 gagaattttc atgaaatcga tggcggttta gatttttatg caccccagac ctgtgaaaac
     7801 gaagcaggcc aaagaataat gattgcttgg atgcaaatgt ggcagcgtaa aatacctacg
     7861 aatgatctgg gccatggttg gtcaggttcc atgaccttgc ctagagagct gagggtggct
     7921 gataatcaat tgttgcaacg gccagtttca tcaatttatc aagtaattga tccgcaggaa
     7981 aggattgaat gggcgaagac tgaagagaat cgcttcttac tgaaggactt agtatatgac
     8041 cagcactatt tacgcttgga aatcaattta aaacaggcaa cgaaactaga acttcactat
     8101 gcgaagggac ccgaccatac attactgctt aactatgatg ttgaacggga gtgctttact
     8161 gcttccaggg aacacatgcc gtatgattta tcaggagctg aaaaggaagt gttgaaaaag
     8221 cgcagtgtga cgattccttt ggaaaataat tgtctaacca tcgaaatttt ccgtgatact
     8281 tcagctattg agctttttct aaatgatcgt ctgactatga gtacaacttt ttatgagaca
     8341 gaaaaaggac aggattttct tttgctggca gacagtgaag taaccttttc attggaaagc
     8401 ggcttaatta atctatagaa gggagagctc agtgatgatt tatggttcaa tcgaagcagg
     8461 cggcacaaaa tttgtgtgtg ccattagcga tgaagaaatg acgatcaagg aaagagtgag
     8521 ctttccgaca acgacacctg aggaaacaat acctttagtc attgacttct tcaaacaata
     8581 ccaagcagat ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga
     8641 ttccgccact tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DADQRX010000105         8546 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.109, whole genome shotgun sequence.
ACCESSION   DADQRX010000105 DADQRX010000000
VERSION     DADQRX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.109"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="J8V14_002391"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="J8V14_002391"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBA0922470.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="J8V14_002392"
     CDS             complement(1098..2201)
                     /locus_tag="J8V14_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBA0922471.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="J8V14_002393"
     CDS             complement(2291..3226)
                     /locus_tag="J8V14_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBA0922472.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="J8V14_002394"
     CDS             3528..5462
                     /locus_tag="J8V14_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA0922473.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="J8V14_002395"
     CDS             5919..6335
                     /locus_tag="J8V14_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBA0922474.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="J8V14_002396"
     CDS             6349..6915
                     /locus_tag="J8V14_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBA0922475.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="J8V14_002397"
     CDS             6938..7924
                     /locus_tag="J8V14_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBA0922476.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8546)
                     /gene="scm"
                     /locus_tag="J8V14_002398"
     CDS             complement(8079..>8546)
                     /gene="scm"
                     /locus_tag="J8V14_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBA0922477.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2519 a         1643 c         1735 g         2649 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggt aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt tacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgtggt agaggtagta
     8521 tctgtactgt cacttgaagc tgatgt
//
LOCUS       DADQRX010000106         8494 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.110, whole genome shotgun sequence.
ACCESSION   DADQRX010000106 DADQRX010000000
VERSION     DADQRX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.110"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="J8V14_002399"
     CDS             complement(<1..173)
                     /locus_tag="J8V14_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0922478.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="J8V14_002400"
     CDS             complement(593..1714)
                     /locus_tag="J8V14_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBA0922479.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="J8V14_002401"
     CDS             2042..3451
                     /locus_tag="J8V14_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBA0922480.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="J8V14_002402"
     CDS             3448..4176
                     /locus_tag="J8V14_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBA0922481.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="J8V14_002403"
     CDS             complement(4304..5215)
                     /locus_tag="J8V14_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0922482.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="J8V14_002404"
     CDS             complement(5232..6239)
                     /locus_tag="J8V14_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA0922483.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="J8V14_002405"
     CDS             complement(6236..8365)
                     /locus_tag="J8V14_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA0922484.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="J8V14_002406"
     CDS             complement(8370..8477)
                     /locus_tag="J8V14_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922485.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DADQRX010000107         8295 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.112, whole genome shotgun sequence.
ACCESSION   DADQRX010000107 DADQRX010000000
VERSION     DADQRX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.112"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(90..728)
                     /locus_tag="J8V14_002407"
     CDS             complement(90..728)
                     /locus_tag="J8V14_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBA0922486.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(851..1483)
                     /locus_tag="J8V14_002408"
     CDS             complement(851..1483)
                     /locus_tag="J8V14_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBA0922487.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1834..2133
                     /locus_tag="J8V14_002409"
     CDS             1834..2133
                     /locus_tag="J8V14_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA0922488.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            2628..4793
                     /gene="acm"
                     /locus_tag="J8V14_002410"
     CDS             2628..4793
                     /gene="acm"
                     /locus_tag="J8V14_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBA0922489.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(5065..5550)
                     /locus_tag="J8V14_002411"
     CDS             complement(5065..5550)
                     /locus_tag="J8V14_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBA0922490.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            6030..6671
                     /locus_tag="J8V14_002412"
     CDS             6030..6671
                     /locus_tag="J8V14_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA0922491.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            6690..7325
                     /locus_tag="J8V14_002413"
     CDS             6690..7325
                     /locus_tag="J8V14_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA0922492.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            7343..8158
                     /locus_tag="J8V14_002414"
     CDS             7343..8158
                     /locus_tag="J8V14_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA0922493.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2970 a         1267 c         1581 g         2477 t
ORIGIN      
        1 ttttaatcca ttgatacatc tgtctttttg tttaaaggaa ataaagtatg ctttcttagc
       61 aatagagcat gtattatttc tataaaaatt taggagaata aaacgactag caatatagct
      121 gtgaaatcaa atatcacatg aactgcccaa cttgtaatga tcgagttgct tttaatggca
      181 gcttggttga ataacaatct tgccacccct tgtatcaaaa ggatgtgtgc tacagtgtgt
      241 aaaatatttc cattatcata agtactgtaa tggataaatc caaagatgat tgctgacaag
      301 aaactagcga caggaatgct gaacttacta gaaaaaatag cgagaaaata cactgagaac
      361 aattcttctc caagtatcat gataggcaaa ataataaata ataacgggct gaaagcctca
      421 tttattggat tgcctgttaa accccatttc aaaatttgtt gcaaaaatac cgagactgcg
      481 gcactgatta ctacattgat aaaaaaatat ttggctatat ttttgacagg agcgacaggt
      541 ttcgagaaca tttttttcgt atctttttta ccaaaaacaa gtgtaaatgc tataaatcca
      601 gtgatagtta aagaaaaaat gctaataatg gatggcgcaa atagcaaccc taacaaaccg
      661 agtacaacga caatagtgcc caatatccct tttttaagtt ctattttttt tcctaaaatg
      721 ttattcatga taataactcc tactattcag tttttgaaga tagagtcagt atacatgaag
      781 agttaaaata ggcaatgttt ttttatggat atttgtattg ctaaaaattt atcttatata
      841 ttcattttga ttatactact tttatttgta aaaattttct agcaaaacca agtgcttgca
      901 actgattaag cgtacaattt tttagatgtt ccgagcgtac ttcaatcatt tcaaaggagt
      961 tgctacttaa atcgatattt tttaaatcag tatcaaaaaa gctagcattt tctaaatcat
     1021 tagtagaaaa cattatttta gtgagggaac agtctacaaa atctgcgctt gataagacag
     1081 agtgctcaaa ggtactttct tttatgtttg aaccagataa atttacatag tccattttac
     1141 aattctcgaa taacgtgttc ccaatataac tttccatcaa tttagtacct gttaatttac
     1201 aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa tatacagcct gaaaaatcac
     1261 agttttcgaa tatacagtcg gtaatctcca ttctttgaaa atcattggaa gcaaaaaatg
     1321 catacttgaa aatagatttg taaataattg gatggcagat tatttggttt tcgatgacct
     1381 ctttttgtga aaggatatta tacagttcaa aatcttcgtt ttcatagata tgaaaaaagg
     1441 atcctttatt caagcttgct tcattaactt taggaaaaat catatttaat tacctccatt
     1501 ttcataaagt catattaaca aaagctaaac caacttacat tagcagaaaa tgagataggt
     1561 tttcgccttt ttcgataacg atagtgtcgc tatctcttga aacgttctta gaattgccct
     1621 gagagacaca aattaggcgt aattctaata aaaatcttag aataaaaatt tttactgatt
     1681 taatttagaa tgtttttaaa ataaaaatcg ttgccataac agtgtattga aaggttttaa
     1741 caatccagta atgatttaaa aaaatctaga tgtagcgtat actttgttta tagaatgtag
     1801 tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta aaagtgaaga aatagaaaac
     1861 atggtactaa aattatatga tctcgtttta aatccagaga tcaaagaaaa ggaacgtata
     1921 cttttgatag acgcaaagac aggcttagaa aaaggtcagt attatcctaa agtcataaat
     1981 aatttggaac gtagtttacg tccgttagca atcaggggag aattgtctaa accagtatca
     2041 ccgttttata tggagatatc aacaatagga aaatttgaaa aagaattagg tcgaggaatg
     2101 gcttcggcac cgattacttt tgggcattta taaaaacgag ttattcatct caatgaatta
     2161 aaaagtatag tcaatctcat aatgcaggct aggttcattt atttgaacag agtctgtatt
     2221 tttttattct aaataaagaa acagatgttt ctttatattg tattttatcc tttaattaac
     2281 acaaaaacag ctagtataaa ctaataatgt taattaaaag tgtattttat gaaactatcg
     2341 ttatttatta atgttgcatt tttgtaagtt aaaaattgga aatatagatt gaaaaaatga
     2401 aagcaataag taaaataaaa tgaatatatg cgaaaaaata atttacatta ggaaaaacct
     2461 tgtaaaaaca gttttttggc atatttattt tttataaata tttgtaaaat aactatcaat
     2521 tttttagtta aaagattgga aatgagtggg ggaggaattg aagaaaggat ttttgagaga
     2581 tgatatagta ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta
     2641 gaaatattat tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc
     2701 atgtctatgc ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc
     2761 caacgaatat tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac
     2821 aaaatatcca accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg
     2881 gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa
     2941 cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag
     3001 tagaaggttg ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa
     3061 atacgggacc attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc
     3121 cagcttctgg ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag
     3181 atactaagca cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac
     3241 cagttaaaat cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa
     3301 ttaatcaaat acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat
     3361 ttttacaaga tttccccagc gctacattta atttttcagt aactgataat tacattgaaa
     3421 taactatacc aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca
     3481 tagaaaaccc agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta
     3541 ataaacctgc tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg
     3601 gtggagtcaa cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag
     3661 agattggtat cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag
     3721 gacagtctag tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc
     3781 aagtaggaga ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac
     3841 taagttcaaa tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac
     3901 ctatttataa caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa
     3961 ccactccaag accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac
     4021 cagttcctga tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag
     4081 atatccaaca gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc
     4141 aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta
     4201 caaatgagaa taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga
     4261 accaagacgg caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga
     4321 tccaacacaa agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa
     4381 aatacagaga cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca
     4441 gtacgacgat tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat
     4501 ctataccagg tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga
     4561 aaattagaaa tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg
     4621 aacaaagaag caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa
     4681 aaagcattct tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat
     4741 tgatagtaac tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat
     4801 ataatatttt ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga
     4861 aaaaagaagc atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc
     4921 taactatgga agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg
     4981 attggccaaa agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg
     5041 ggaaataaga aaactactct atcattacaa attttcataa actggcagat gttctaagta
     5101 aacaagtatc ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg
     5161 atgaaagcct acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt
     5221 ctctttgtcg tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc
     5281 aggaagctgt tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg
     5341 ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct
     5401 tgatttcaat aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac
     5461 tgacgtaatc gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc
     5521 ataagcttct tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt
     5581 atcataaaaa agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac
     5641 aaatgacatc gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga
     5701 aagaggcagc agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag
     5761 gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct
     5821 tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg
     5881 gatcaaagga ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa
     5941 ggaaaaaata aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg
     6001 atttttttat tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat
     6061 tattagtagg agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac
     6121 cattagggat tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc
     6181 ttcaaacata tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt
     6241 tttatgggtt gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct
     6301 ttatcttaaa ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc
     6361 ctcaaggtca atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga
     6421 aaatcgtcat cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca
     6481 acttggtcaa agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg
     6541 ggaaaatcgc gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct
     6601 atcttacatt gactggtatt ctaacaatca taatgaaaca agttgaagga aaattaaatt
     6661 attaccgcta actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat
     6721 ttggtgaaaa aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag
     6781 ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag
     6841 aaagttctga cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta
     6901 aaatgaaaga acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga
     6961 atatttttga aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat
     7021 acaccaaaaa agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct
     7081 atccttatca gctttctggc ggacaaaaac agcgggtagc tattgccaga gcattggcta
     7141 tgaatccacg ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc
     7201 agcaagtcga gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca
     7261 cccatgattt agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc
     7321 attaagaaag gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg
     7381 tcttcttctt tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat
     7441 caacgaagag aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag
     7501 agataaagac gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca
     7561 atatgggatt acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa
     7621 caatggaacg atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa
     7681 agtgcaattt acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc
     7741 aggaatcaca aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc
     7801 tggttatgat gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga
     7861 tgctgttttg tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga
     7921 tggtctgctg atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga
     7981 agacttcaac ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa
     8041 gtcagatagt acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg
     8101 tgaattccaa aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca
     8161 aataggggac tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc
     8221 ctgccaactc cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg
     8281 ttcaaaagcc agttt
//
LOCUS       DADQRX010000108         8243 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.113, whole genome shotgun sequence.
ACCESSION   DADQRX010000108 DADQRX010000000
VERSION     DADQRX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.113"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            229..1554
                     /locus_tag="J8V14_002415"
     CDS             229..1554
                     /locus_tag="J8V14_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBA0922494.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1547..1897
                     /locus_tag="J8V14_002416"
     CDS             1547..1897
                     /locus_tag="J8V14_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922495.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1879..2064
                     /locus_tag="J8V14_002417"
     CDS             1879..2064
                     /locus_tag="J8V14_002417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922496.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2968..3243)
                     /locus_tag="J8V14_002418"
     CDS             complement(2968..3243)
                     /locus_tag="J8V14_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBA0922497.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3215..4168)
                     /locus_tag="J8V14_002419"
     CDS             complement(3215..4168)
                     /locus_tag="J8V14_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBA0922498.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4780..6273
                     /locus_tag="J8V14_002420"
     CDS             4780..6273
                     /locus_tag="J8V14_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HBA0922499.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6408..6704
                     /locus_tag="J8V14_002421"
     CDS             6408..6704
                     /locus_tag="J8V14_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBA0922500.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6805..7656
                     /locus_tag="J8V14_002422"
     CDS             6805..7656
                     /locus_tag="J8V14_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922501.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7677..7889
                     /locus_tag="J8V14_002423"
     CDS             7677..7889
                     /locus_tag="J8V14_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922502.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2863 a         1283 c         1552 g         2545 t
ORIGIN      
        1 ttctaatata actaagcaaa actggataat acgacactta ttttcaaatt ttcacaagac
       61 taatagtaaa tatccattta ataaagtgat acaatgtgtt gattttacaa atcaacagaa
      121 gaagtaggga aataacttac ctatgcttct ttttttgtac atttaatcga ttcttcttta
      181 caaaaacgaa cgtatgtttg tataatgtgg aagaatggag tgttatttat gatggattat
      241 tcaaaagagc cagtaaatga ttactttttg atcgatatga aaagctttta cgcaagtgtc
      301 gaatgtatag aaaggaattt agatccatta acgactgaat tagtcgtcat gagtcgagcg
      361 gataatacag gatcagggtt gattttagct tcttcaccag ctgcaaagaa gaagtacggt
      421 atttctaatg tcagtcgacc acgagatcta ccgcaacctt ttcctaaaac gttacatgtc
      481 gttccaccac gcatgaatct ctatattaag cgaaatatgc aggtaaacaa tattttcaga
      541 aggtatgtag cagacgaaga tttattgatt tactcgatcg atgaatcgat cttaaaagtg
      601 accagatcaa tgaatctttt tacgactgaa ggaacaagaa gtcaacggag aaagaagctg
      661 gctcaaatga tccaggagaa tattaaagaa gagctaggat tgcttgctac agtaggtgtc
      721 ggagataatc ccttattagc taaacttgct ttagacaatg aagcaaaaca taatgatgga
      781 ttccttgctg aatggacgta tcaaaatgtt cctgataaag tatggaatat tcctgaaatg
      841 acagaccttt ggggtattgg atctcgaatg aaaaaaagac tcaaccaaat tggtatcatg
      901 agtattaaag acttagctaa ttggaatcca tacactatta agagccattt aggtgtgatt
      961 ggcttacagc tgtattttca tgcgaacggg atcgatcgaa cagatatagc gatcccacca
     1021 gaaccgacaa aggaaaaatc atatggtaat tcacaagtgt taccacgaga ttataccagg
     1081 agaaatgaaa tcgaactggt ggtaaaagaa atggcggaac aagtagctat tcgtatcagg
     1141 caacataact gtaaaacagg ttgtgtccat ttatacattg gcacgtcgat cttagaaacg
     1201 agaccaggtt tttcacacca gatgaagatc cctataacag ataatacaaa ggaattgcag
     1261 aattactgtc ttttcttgtt tgataagtat tatgaaggac aagaagtaag gcacgttgga
     1321 ataacctata gcaagcttgt gtacactgat tcgcttcagc tggatttatt tagtgatcca
     1381 caaaagcaaa ttgacgaaga aaatttagat aaaattattg acaagattcg gcaaaaatat
     1441 ggttttacgt cgattgtaca tgcttcttct atgttagaag gcgctcgtag tattactaga
     1501 agtacattgg ttggtggcca tgcaggcggc gcaggagggc ttaaaaatga ttgattataa
     1561 tgatcgtgga cgtttaaagt gggcaggttt ttatttatct gatcatactg aaaaaattga
     1621 tgccgacaat gaacaacgag ccaatcaaaa tttggcaaaa gaacaaatga cgactgaaga
     1681 ggttgcggaa gtattgaacg ctgcagtcat aaaaaataga tcagtatgta ttcaaaaaga
     1741 agaacgaaac gctgaaggat actatccaga agatattgtt agcaagatct taggtagtga
     1801 tgagctgggg atctacgttg gaaaaaacaa agttcactac gacgaaatac gtcatatcga
     1861 attttaccag gataataaat ggggtcgatt agaatgaaca ttatccagca atatgagcta
     1921 aagtatataa cctttgatca gctatcagaa gagatttggg gttatggcca gcgtttgata
     1981 aatgaggttg gtgttgaacg cttctctttc tatgtagaag ctgcagcagg ataccataaa
     2041 tacgaatact atattggcta ttaaagtgca ataaaattat atttctttta tgttttacat
     2101 tttgtatcaa ttctgataaa attacctata ggtaattaaa aaatataaaa aaactagtta
     2161 caaccttagg acgttgtaac tagcttgaag catactagag ctaactaaaa tagcactaga
     2221 aagcaatgtt aaacccgtcc aacaaacttg atttaacatt gagaccagct tattcagcta
     2281 aatatagttg aataagcttc ttttgtttta aaaaaacaaa aggtgtacta cgaataacgc
     2341 aagtaaaaga aatactatta caaccagtag gctacaagta agtattacta aacattgcgt
     2401 agttagaacc tttgctctat tgtctaaagc cacaaaatta gagatcagct ttatgatgct
     2461 aagagcgagc tgcatccatg cggacatagg ttcttttgac actattaatt ctccttttta
     2521 ggataaggga aattgctagt agttttttca ttagcaccat cccccttcat ttgattgagt
     2581 ctagtgctgc tgtgatagtt tgctgtttaa tatgcttaca ttcttattat aacataaatg
     2641 aaacaaacac atggttagag gtcgtatcaa tcaaaatagt gtctcacaaa cgatttatta
     2701 cttactcctg ctgactaacg gactgttctt cagtaactgt aactatatgt ttgttaaaac
     2761 caccattacc tgtagataaa aaccaatata tttactatac tcactatatt aaatatattt
     2821 agtatagtaa aaactagaaa caataaaatt tgtgaatgtt gtataccccc aaacaataca
     2881 catattttga aggattagga ttaataaagc taaaaatata gtaagtatac taaatatatt
     2941 tactatatct agtatactta ctatatttta atcttttgat cgtctattat atataatccg
     3001 ttcctcgtcg tttaaagtat caacataggc gtccattgca agtttgacta tttcatacat
     3061 aggaatatct tttgcataac taagatcccg aatggttttt agtacgggag gatctacttg
     3121 aactggtttt ctttctttcg cagtgtactt tcgttcccct aactcgacat taatatcatc
     3181 tttaattgaa atatcttcag caccaatttt cttcttatct ttgttcgttg gtaataatgc
     3241 catttagttt aatctccttt ccaagaggta ggatatgatt ggccattgag tcagcatatt
     3301 taggtgtata atcaaatcct tcaatatcac catgttgttc atagtattca attctttctt
     3361 ctaattctgt aaagatgtct gcaaaaatag cccaccatct tcgatctaca taatcattta
     3421 attggatacc attctcacca tacatttcta accgatccga gcctttaaca atgcttttaa
     3481 aaatgtttga ttcaccatac ttatcaattg ttttttgata gtttgcgtgt tgagaggaac
     3541 gtcgcactga gaaaagaaca ggcaaaattc caatgatttg aagatgagac tcttcaaaat
     3601 tcgtgacaat cggaactaaa actttattaa taaaatcttc tgttccttcc attgcgtacc
     3661 gttttaattc ttgcattgca ataatataat ctgcagcagt taagaatgca cgaaccacat
     3721 tatctgttga tggtgggcag tcgataaaga tataatcgaa attttttctc aattcttcta
     3781 aaggtttaat gaaggccaat gatctcttac gacgatcagg atatttttcg gacaagtatt
     3841 cattaagttc ttcagatcca ggagagccag ccataatata taaatgtgga cttacttgag
     3901 taatacctga aacccagtcc ccttttttga ttgctgcaga catggatcga tcaaagctag
     3961 taatttgata cgttgatcca atgttggaag tagtatttct ttgttggtca taatcaatta
     4021 gcaacacgtc tttttcaaaa agttgactag aaacaatcgc taataaatca ttgtctgtag
     4081 tcttaccaat gccgcctttt ctacctgtaa gcattacaac ttttcctagt ttatctgatg
     4141 ttttaaatgc agtccgtaag ttttccatag ttccctccaa tttcctatac taaatatatt
     4201 tagtatactt catatactta atatattcga tatatctaat atactccaaa ttgataagaa
     4261 ttcagacttt ataaggaata tagtggatat actaaatata tttgctatat tcagtatatc
     4321 agatatattc aaaatgtcaa aatatatttg tgcgttttgc gttgacaatt tagagtgaaa
     4381 agaggtaaac ttaattctat agaaaccaca gataattcac tatttacaaa gtagcaaaag
     4441 cagtggtact ctttaaacag aaaaattaca taaaaaaaag agacggaaat aattgatttt
     4501 agcagaatca attatttctg aatcacatgg ttaggacatg tgttcacgct cgtctcaaga
     4561 atatgtaagt agctaccaac tacttatatt aaaagttgat cggatacctt ttccgattgc
     4621 ctttattcta ccaaaaaaag gcgagcaact caacagaaga acgtttattt agtgtactta
     4681 aattaaatat tcttaaacac tgaagagaga acacatacta tggctgagat taaagctaaa
     4741 ctgtgttctc tttttgtttg agaaaggacg ttacaatcga tgttgagcga aatttattca
     4801 gctattctta aaaacggctt acgtacatac aaatatcgaa acagcaccat gaaacctgca
     4861 ggtcatattg attccagtag gagtggtgct atctttgcgt accgtagcaa gcaattaatg
     4921 aatgttgggc gtggtatggt cattacttcg gaagaagcta ttttagagaa cgaaaagaaa
     4981 ctaacgcatt ggacacctaa tacctatcgc tttggtactt atgcggatga ttatcgaaca
     5041 gtggttaaag gtcattctga gaaaaactta agtcaaatca acacatttgt agttgatatc
     5101 gattcaaaag agaaccatca aggcgaaatt attttagcct gtttagatca agtgggctac
     5161 atgccaacgc taattttaga atcagatcat ggttatcaag tttattttgt actaaagaca
     5221 ccaggctatg tcacaaagaa aagcaatttc aaggtgatcg atgtagcaaa aaaaatttca
     5281 aaaacaattc gtctacagtt ggctgaaaaa ttgtccggag tggatctagg atgtaaccac
     5341 ttcggaattg ctcgattccc acaagaaaat aatattgttt ttaaagaaat gaactatcag
     5401 tattcttttg ctgactggat tcaatggtcg atgaagcttg aatcagatca aaaaacaaaa
     5461 gaagatcgag aacgaaaact aattgtcttt cctgagaaaa aagaaattcg tcaagtagat
     5521 gaaccgtggt tcgatatgtt acttcataaa gcaaacatcg tcggcggaga aggacgtctt
     5581 ggtcgaaata atgtgatttt cacgctttct ttagcttatt actcttcagg ttatgcattg
     5641 gatacttgcg aatacaatat gttcacattc aacgaacggt tacaggaacc gctatctgaa
     5701 gcagaattag taaagattgt acgtagtgct tattctggta attatcaagc agcaagcaga
     5761 gaattcatta ttgaattgtg ccagcaatgg gtttcaccaa ccttaaaaaa taatgatctg
     5821 ttcattcaac gacgtggctg gtggaaattt aaaaagccac gagatcaaag aaaatatagt
     5881 cataaaaatg aatggaaaga agatctattg gcttatctaa gcgaaaaaag tcttgctagt
     5941 gagccttatt tgaacctgtc aaaaaaagaa ttggcttcac aactaacgat gcccaagcga
     6001 acacttgata agatcttaaa tgagttacga gaggagaata aagtcttcta caaggtaaca
     6061 cgaggaagaa atggcggaat gatcattgct tcagtcaaaa cgttgttcgc gagggttatt
     6121 caactaagta aagaaaagag actagattat tcaacaagga tcacagaggt atttggactc
     6181 aacgagagct tagtagaaga gacatttaga caattatcaa cccccttgaa ccctcttaaa
     6241 gaagtgaatc tttttgaagt agatacaggt tgaaaaataa aaggtgcaaa ttgccctata
     6301 caatatatct atgatacttc tgtaggtgtt gctccctttg cctagctacg tgtaggtggt
     6361 atacttataa ctgcgtatgc agtcttatgt atcatggagg aagttgtatg tcgttaaaaa
     6421 attatgtcta ttcacatcct gttaatgtgt ttatcattgg tcgtttggga ttatctgtgg
     6481 aagtgttctg tgagctttat ggttttaagc aggggacttt gtcttcttgg gttaccaggg
     6541 aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg gttgtcctta gcttctgggg
     6601 agactatgga tcaggtatat gattgtttgt gtgtgttaga gcaggagtat attgagtata
     6661 aaatagctaa cgagttaaga aaaaggaaga agtatatcca gtagtagttt ttatttactt
     6721 aaaaaattgg ttcattgagc aacctatcgc tgctaaagta gcaaaggaaa acgttaaagg
     6781 aaagaattta gggaagaaat cgaaatgaaa tcggacgact ggattgaaca cacctggctt
     6841 cctctgaaaa gaaatagcaa acgaaagaaa ttttgggtat tgcaattatg gccattagga
     6901 aaactttgta gttacggggc acgacttatt cttgatttaa ccgtttactc gattggagta
     6961 tttggaacgg cttattactt gataaatgat cccaatagga ttgcagcatc agacattttt
     7021 acagtgctaa tgacgttgat ttttgtcatg ctattgtcta tttttactca attagtcatg
     7081 caatttacca ggcaaaaatt aacagcagga aaacttttca aaagcctatt cgatttaatt
     7141 gttgtaaatg caattctagc gactttaata aaacaaattg aacaatctgg aagaacaaca
     7201 aaaggagtga aggatttttt attgacgttt attcactttg taaatggaca catgtatttg
     7261 tttgaagtgg gtattattct cttttttgtc gggtatgtat ccacgaaact tattaaacgt
     7321 tcaattgaaa aagaaattga aattgacctg attccgacac ttcttggtta tgagtacaat
     7381 gttgattttg gaagatatga cgaattgatc aatacggata tcgttcacac gagtatccat
     7441 gaatctactg ttaagggcat cagatctatt ttaaaaaatg gccaggaaat gaaagcaata
     7501 gatcgaagaa aagagatcta caatttttat gaagtcgaag caagcctacg atatggcatc
     7561 ggaaaattgt ggttagatgg aagtaatatt cgtcaaaaca aaccatacat tttttggaac
     7621 agtagtgatt tagaacttcc aatgaaagat aaataattat ggagaatcgt ttgaaaatga
     7681 ttcctctata caggattgtg cttatagttg gcatggagtt aaaaacctta aagaaggagc
     7741 agttattcaa agtgaaagaa cgtcataatc agttactatg tagtaaaacg ggtacgatga
     7801 acattgaata ccgaacagaa tatttagaat taacgaatca aactgatttt ttgttgattc
     7861 gaaagcaaca agagcaagaa aaaaattaat ggctacttat agccaattga ttgatgaatg
     7921 ggaacaattc aaggaatcta aaattccaga acgtctctaa aagcctttat ttgtaaagag
     7981 cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc aataggctgt gcctattacg
     8041 tttctcaaaa ataaatgtgg gaacaaaata tttaaaaata ggatagtgaa aggatagtga
     8101 aggatagtaa aaggagggta gtaggatagc aaaaaggata ggctcctttt cgctatcctt
     8161 tttaaattgg aggaattaat atggaagtaa gaataagaaa tgtggatgag aaaatcgtaa
     8221 gagaaattga tcgaagatgt gac
//
LOCUS       DADQRX010000109         8169 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.114, whole genome shotgun sequence.
ACCESSION   DADQRX010000109 DADQRX010000000
VERSION     DADQRX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.114"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..727)
                     /locus_tag="J8V14_002424"
     CDS             complement(<1..727)
                     /locus_tag="J8V14_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922503.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGI"
     gene            complement(935..1861)
                     /gene="glsA"
                     /locus_tag="J8V14_002425"
     CDS             complement(935..1861)
                     /gene="glsA"
                     /locus_tag="J8V14_002425"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBA0922504.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1884..3311)
                     /locus_tag="J8V14_002426"
     CDS             complement(1884..3311)
                     /locus_tag="J8V14_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0922505.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3342..4556)
                     /locus_tag="J8V14_002427"
     CDS             complement(3342..4556)
                     /locus_tag="J8V14_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBA0922506.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            4906..5985
                     /locus_tag="J8V14_002428"
     CDS             4906..5985
                     /locus_tag="J8V14_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922507.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(6286..6528)
                     /locus_tag="J8V14_002429"
     CDS             complement(6286..6528)
                     /locus_tag="J8V14_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA0922508.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(6560..7117)
                     /locus_tag="J8V14_002430"
     CDS             complement(6560..7117)
                     /locus_tag="J8V14_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBA0922509.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(7130..7318)
                     /locus_tag="J8V14_002431"
     CDS             complement(7130..7318)
                     /locus_tag="J8V14_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBA0922510.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(7331..7897)
                     /gene="amaP"
                     /locus_tag="J8V14_002432"
     CDS             complement(7331..7897)
                     /gene="amaP"
                     /locus_tag="J8V14_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBA0922511.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         2427 a         1777 c         1511 g         2454 t
ORIGIN      
        1 gaattccttt ttgctcttcc aatccttgac agactaattg aaataacggc ttcgtttctg
       61 cgcttttctt atcttcttct ttcagtccaa ggggaaatgc ctttcttccg attgcgatct
      121 ctattcctcg tatgataaag gacttttcat cttccgaata tcttctttta aaaaaatgag
      181 tctctacata atcaatcaac tcgttacaag cttcttctac atacggattt ttattttcat
      241 agccttccca attgacataa cgtactaaag ctagcaagat gttccgcgag tcaaattctg
      301 ggataatgat ttctttattt ttcaaataat cattttcttt aaaaaaatcc aatacgattt
      361 ttttgattgt atatactttg ctcaaagaaa taaattcaag atcagccagt tctgtactcg
      421 tgaatatacc attaaaataa taacttaaaa actttaaaaa ataagattga ccgtacagaa
      481 actgtgtgag ctcagaaaga gcaataccat gtttgaatac gagtttatac ttatgcttct
      541 ctcttacttg ttcttttata caatattcta gacactcggc tagactttct agatcgtgaa
      601 ggatcgttgg tgtagtcacc cttagagaaa tagctgtttg ttcgatgtct atttcttgga
      661 attcaaataa ttgttcacac agatacactt ttctaaatat atttcgctcg atgtaatttt
      721 ccagcattcc tgatacacgc ctccagtttt tcttatgatt ttatttaatc atatctgtaa
      781 atcgtatttt tgtttccttt ttatttagaa aagccctttc aaaaaccaat aaaaaaagaa
      841 actgggacaa aagtcaaaca agctcacaat ttcagcctca ttgtaggtag ctttgagccg
      901 tttggacttt taccccagcc tctttcaaga atggttattc aaagatgtca gcgtctagtt
      961 ctttgactac atctttcaac aattggatac cagctgcgct gttaccaaat ggatctagtg
     1021 ccgggctgaa gacacctatg ccgtaacgat ttggcacagc tgccatcaac ccgccgccta
     1081 caccgctttt tgcaggtaca ccaacatgga cggaaaactc tcctgattca tcatacaaac
     1141 cagcagttgt cataattgat ttgacgattg tagcactttc ttcagtaatg atttgtttgc
     1201 cattccaagg agcaattcct ttatttgcca gcacagcagc taattttgct atgcctgtag
     1261 cggtgacatt gagggagcat tgtctgaaat aagcatcgag tatctccggt acgtctcctt
     1321 cgatcatttg attacctttc atatagtaag caagagagcg attgatatca cctgttcttg
     1381 attcagaatg atagatttct tcattcaatg tgatattagg atcatcacag atctctttca
     1441 taaatgacaa gattcgctca aatttttcat ctgcatcttt accggcaatc aacgaggttg
     1501 ttgcaatcgc tcctgcatta acaaaaggat tcattggatg tttgcgatga ttgatttcca
     1561 tattcatgat agaattgaag gcaaaaccgg ttggttccgt gttgattgtt tgaaagactt
     1621 tttctattcc gttgtctttg attgctaata acaatacggg aacttttgac atgctttcga
     1681 tggcaaagcg cacatcggcg tctcctccct caatcagttg ttttgtatcc aaatcataaa
     1741 tcgccatacc taattgtttg ggatccactt cagctaatgc tggaatgtaa tccgccactt
     1801 gtcctgtttg ataatagtgg aaattttttc caatcaattg ttctaattct tttacttcca
     1861 tttgaataac cagctttcat tttttatttt tctgcatgct ggaatttatg acggcatgcg
     1921 tagatcgtaa atggaataac taagacgata agataagaaa caatcagaat cgtttcataa
     1981 atcccatgct gtccagctgc gatactagca ggtggaacaa atgagataca aaaagcaaaa
     2041 agtgaagtca aaagacctaa acaagcaacg attactttta caacttttcc gcctggaacc
     2101 tgataggctc tttcaagact gtcatcttta aaaaccagct tcaaatagcc cgcgaaaaac
     2161 ataaagtaca acatcaggta gattacaaca gtcaatgaca tcgctgcaag aaatgaaaga
     2221 ttattccccc cgccgcttaa tgtaaagacg gctgcccaga ttgttgcaac gatcccattt
     2281 gcgatcatca aatgagcagg aacaccgtgt gtgttttctt ttgcaaaata ttcaggaagt
     2341 aatccttctt ctgctgctac gtgaagtgct tttgaaggag aggttaccca agaagcaatt
     2401 tctccgatga cacctaaaca aacaagaatc gcaagaattt tcgcaatcca tgcaagattt
     2461 ggatcaatgt gtcttaatag gaattctaac gattgaatca cccctgtatt aagagacaaa
     2521 ccatgttgag gtactgtagt agcgacggtc aatccgccaa aagtatctaa aatgattgcc
     2581 agaataacca atacaaacat tgctaatgga taatcctttt tagggttttc catttcattt
     2641 gcatgactgg cagatgtttc tacgcccata tatgctaaaa taaaggaaac aaaaacaacc
     2701 agcgtaggca atttcgaaaa atcaggaatc aatgttttca tagacagtat cgtttgtagt
     2761 ggagcgcctg aagcgacata atggatccct aagccaaaca agactaatgc tggcaacaag
     2821 ataccagcta caaaaccgat ccgagcaatc aatgaagtgt atttggttcc ttttaactgt
     2881 gagagtaaaa tagcccagaa aataatcaat aggattgcca atttcagata tttattgttt
     2941 tgtatagcac caatacccgt tgcataggat aatgcaccag ttaagaaata cagcatggtc
     3001 aagtagccaa ctgttatctc aaaccattgg aaaaagattg cggcaaaacc gaatcgtttt
     3061 cccaatgtgc gactgaccca agtatagaca ccgccttttt cccatccttt caccgttgcc
     3121 atctctgctg cgcacaatgc cacagggata aaccatagca aaccgcctaa taacaagaaa
     3181 aatactaagc tgaagcctga ggtcgcgaat gtgggatatt catataatga aacgaccatt
     3241 gagatggtca tagccaaaaa gccaaagaac gttaattttt ttgcaggaat tttcgttttg
     3301 ctttgttgca tgaatcttcc tcttttctat ttgaatttgt ttcaattgag tttgttgcca
     3361 aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg tagtagtgat cgttgtaaag
     3421 tgcatcgccg attttaaaat cactgttgtc agcacatagg catacacggc agtaatgatg
     3481 acaccaatca cttgaatccc taagaagtac cagctgcttg gattcgttag taaatggctg
     3541 ttagatgcaa agatccctat cagaatcgta cctgtgaatc cgcccatgcc atgaacaccc
     3601 cacacatcaa gtgcatcatc ccattgtttc tttttacgaa aatcaacagc agcatgacag
     3661 acgatacccg caatgatccc aatcagcatg gcactttttg cttctacata accggcacaa
     3721 ggcgtaatgg ctgccaatcc agcaacggat cctgttagta cgtcgacaaa atcaaagcca
     3781 tttcctttta cttttgcaat gatcagccaa gtaatcatcg caaatgcaag agcgatgaat
     3841 gttgaaccaa atgcattgac tgcttgttca tctgctcgaa gtgcaccacc ggaattaaaa
     3901 ccgaaccagc caaaccaaag cagtcctgta ccgatagcag ctgccataag actgctatgc
     3961 tgacttcttt ctcctaaagg aatctttctt tgaccaagtg tgaggataca tgctaaagag
     4021 ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga aatctctaaa tcccatctga
     4081 tccaaaaatc cgccacccca tatccagtgg caaacaggga aataaatcag cagattccaa
     4141 aaaataacaa gtataatata gccttttaaa ttcaatctcc ctgcaaaagc tcctgtcatc
     4201 aaaggaacgg taatcacgat aaacatcagt tggtaaagaa agaacattaa aaatggaatc
     4261 gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc tcatcaaaaa gaaatctgcc
     4321 gggtttccaa taacaccgcc gatatcccgt ccaaaggcca atccgaatcc accaaatacc
     4381 cacataatcg ttgttatccc aacggagaga aaagattgag acatgatcgt tagtatatta
     4441 ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag gagtcatcag gcaaaccatt
     4501 gctgtacata aaatcatgaa tgctgtatct cctgtattga taggtaatgt attcattttt
     4561 ttcgcctcct ttctacgatt acgagtataa aagaaggtgt agttgttttt ttcttctgtt
     4621 tttttgaatt aatacttgca tttataaaag acagacaatt aataaaagct tgatgtgttg
     4681 tgctcttttt cgtttttcac acaaataaag acaaaaaata aacggtcttc tttttataga
     4741 aaaccgttta ttttcgtctt tttttatttt taaagatatt ttttcataag gaataaagag
     4801 gcggatttta taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt
     4861 tatattgaag ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat
     4921 gataacacag tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc
     4981 atcctgaaaa aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg
     5041 cagaccataa acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag
     5101 aacgggaagg tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc
     5161 tacgagaaga accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc
     5221 attgaatggg cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc
     5281 tttgccagaa aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat
     5341 cagtggatcg accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc
     5401 cgcaaaccga aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc
     5461 attgaagagc gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac
     5521 acggtcgtag gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag
     5581 acacgcttcg aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg
     5641 gtccgacagc tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc
     5701 tcggacaacg gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc
     5761 tacttcagcc acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg
     5821 attcgccgtt tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg
     5881 cggattcagc gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat
     5941 gacacgttcg tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga
     6001 caacgagcct ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg
     6061 agactaccat gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg
     6121 gtggtaacga gcaaccgggt caccatgggt ggctaactta aacttgaaat ttacgtaagg
     6181 aataaagaat acttcatata aaatatataa aaaagaaccg cagcgagtgt cgctacggtt
     6241 ctcaaatttg ttcttcattt caacataagt ttttcttcaa tttgcttata atttacgtgc
     6301 aaagaatgaa acgactaata ctaaaatgat tgcaccgatc aatgatggaa tgatagccat
     6361 tcctgcaatt gttggtcccc aagttcctag taaagcttgt ccaattgctg aacctactaa
     6421 accagcaatg atgtttgcga tgatccccat tgatgatcct ttattcgtga ttgctcctgc
     6481 aattgctcca atgataccgc cgacgattaa tgcccaaata aatcccattt taaattcctc
     6541 ctaactgact tcttacttct tattgtacac gtggttcgtt attttctttg actttttgtg
     6601 cacctttgcc tacagcagat tttgctttat ctgtttggcg tgaagcaaat tgtcctgtgg
     6661 attttgctgc ttctgttact ttgtcttgta ctgtttcttg atctttttgg tattcttctt
     6721 gtgttttgat gtctgcaaca ttcacattca cttcgataac atctaaatgt gtcatattgt
     6781 ttacttcagt actgatcaat tctttgattt ggttgtaaat atcttcgatg tctttaccgt
     6841 attctacgat cacatctaga tccacagcta cttgtttctt acctacttct gtattgatac
     6901 ctgctgttac gttatctgtg ttgactaatt tttcagccat atttgagaaa aatccgccat
     6961 ctaatgtcaa tagaccatct acatctttca atgcataacc gatgattttt tggatgactt
     7021 tgtcttcata agtcaattca ccattcatca tgcggttttc tcctttttca tttgccaaaa
     7081 ctgatccatt tgttgtattc atgtttttgt tttccatctt tgtttcctcc tatgatttaa
     7141 aaaattgatc aactattcct gactgtttca aataaaaacc gattcctgcg ccaattgctg
     7201 tcatgattat gatcaacaat gtcttgaaaa atccaatcgt taaccataaa acagctagca
     7261 gtagtccaag acccccaaag ataataggga gcttgtatgc tgaaaatagt tctttcatta
     7321 tttggccctc ctattccact cgagagtgct tgtagtctct tgtatcttct tgttcttcat
     7381 aaccgctgta tgtgactgcc actttgaccg tttcttttgt tccgagtact ttagttactt
     7441 cttcttcgat atcttgcatc aatacacctg ttttccctac taatgaagaa gttcgtttca
     7501 agtctccgtt gacatggata tggattcgat ttttcgttga acgtatccgt actttaggtg
     7561 aatgaatgaa ctcttcttcg tgtaaatggg aacgtaccaa cccttcgatc gcttttttgt
     7621 cgattgttag tttgccatct tcccgtttca taacgaaagt tcctcttgac tttggataga
     7681 aaagaacgac tagcaagatg ataatagcaa gaacggcaaa tactacagct gcccagaaaa
     7741 tatattgttg catgtagtaa ttcgttaaag taaagaagcg aaatcgttcc aattgttcag
     7801 gcataatata cggcgcattg atcaacgcaa tcgctagaaa tactgaaagt agcagtaaac
     7861 tcatgataac accgattatt tttaacccct tattcatttt ccacctccta taaacttatt
     7921 tttaccctgt aaataactct accgacaatc acagtataga tagcgaacag ttagcatagc
     7981 atcagtaaat aaagcgctac caaacattgc tggatcataa cggcgtttta ttgccagtct
     8041 gatactgaaa taacaattgc ttacttattc tctatttact tttgattgct ggctatactt
     8101 aaaggatatt attcaagatt aaaaattgca aagattatgc tcagttatta ttacaagatt
     8161 ctatttatt
//
LOCUS       DADQRX010000110         8039 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.115, whole genome shotgun sequence.
ACCESSION   DADQRX010000110 DADQRX010000000
VERSION     DADQRX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.115"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="J8V14_002433"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="J8V14_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBA0922512.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="J8V14_002434"
     CDS             1229..2332
                     /locus_tag="J8V14_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA0922513.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="J8V14_002435"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="J8V14_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBA0922514.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6269)
                     /locus_tag="J8V14_002436"
     CDS             complement(3822..6269)
                     /locus_tag="J8V14_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBA0922515.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6458..7894)
                     /locus_tag="J8V14_002437"
     CDS             complement(6458..7894)
                     /locus_tag="J8V14_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0922516.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2234 a         1740 c         1376 g         2689 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttggttcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgtttctt ttgtgccatt tattatccct catttcatca agtttcatta
     6301 tacaaaaaaa agtaagggaa ctaaagtttt gtttgaaaga actgacgctt tctttcattt
     6361 ttcagcttat tttacagttt ttcttaattt ctaaatgatt ttatcaagaa aaggcacggc
     6421 catatccagc cgtaccttct tgttttaagt atgtgaacta aaggttctct ccattttctt
     6481 caatcagttt ttggtaccag taataactgt ctttcttata tcgttccaaa gatccttctt
     6541 gtgtttcgtc ttgatcaaca tatacaaatc catatcgctt ttgataaccg ttcagccagc
     6601 taagcaaatc tgtataagac caggtgcaat agcctaggac ttctgctcca tccgaaattg
     6661 cttctttgat ggctttgacg tggctttgta agtaattgat tcgatagtcg tcatggattt
     6721 gtttatcttc tgtcaacttg tcatactctc ctaagccatt ttccgtaatc agtaccggca
     6781 tgcggtaacg gcttgtgata cgacgcagtc cgatccttaa tccttctggg tcaatctccc
     6841 agtcccaatt tgttcgttcg acaaatggat tctcaacttt tttatacatt ccaggaactc
     6901 cagtttcttc tgatgaacct ttttttcctg tcgtattcat ttttccagca ccaactccgt
     6961 ttaatggatt gaatgcattc gtcgccgttt gatagtaatt gatcccaaga aaatctggtt
     7021 tagcagattc tagcaattct gcatcgccag cttcaatcgt tggtgcccat cctttttctt
     7081 ctaagaacct catggcagca atcggatatt ttccccacat atagacatcc atccaataat
     7141 tggccatcag gtcttctgca ttttcagcag ccagaacatt ttcaggatta ctgtcaattg
     7201 gataatttgg cgtatacgca aagcttggtc cgatttttcc aggcatttct aactcgtgaa
     7261 atttattgat gacagatgca tttgctaaat tagcaatatg gttaacttca tacattcgtt
     7321 tcgggtctgt cacacctggc ggatgtgctg ctaacaaata acctaatgaa gtgaagatat
     7381 tttgttcatt caaactaatc cagtaatgga ctttcccgcg gaatgcctca aataatgttt
     7441 ctgcataatg agtgaaatct tcaatgatct gacgtgattc ccaaccatta tattcatctt
     7501 gcaatgcttg tggaagatcc caatgatata aagtcaacat aggttcaata tcgttttcaa
     7561 tcaattcgtc gatcaatctc tcataaaaag cgagcccagc ttgattgact tctcctcgtc
     7621 cattaggaaa aatccgtgtc caagctacag agaagcggta agctttcaga ccttgttctt
     7681 tcattaaagc aatatcttct ttataacgat gatagtggtc tactgccaaa tcaccgttcg
     7741 ttcctttaaa tgtctttcca ggtatccgta caaatcgatc ccagactgat tctcctttcc
     7801 catcttcttg ccaagctcct tctacttgat acgctgcaga agcagagccc cacagaaaat
     7861 cttttggaaa tgaggtaagt tctttgtgtt ccattcatct cttcctttcc tataaccttc
     7921 aatcgttcct cttttagaat aacacgaaac cccttccaca acaaaataaa attatgtaaa
     7981 ggaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg aaaatggct
//
LOCUS       DADQRX010000111         7792 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.116, whole genome shotgun sequence.
ACCESSION   DADQRX010000111 DADQRX010000000
VERSION     DADQRX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.116"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(584..1930)
                     /gene="gor"
                     /locus_tag="J8V14_002438"
     CDS             complement(584..1930)
                     /gene="gor"
                     /locus_tag="J8V14_002438"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBA0922517.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2064..3305)
                     /locus_tag="J8V14_002439"
     CDS             complement(2064..3305)
                     /locus_tag="J8V14_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA0922518.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3323..4246)
                     /locus_tag="J8V14_002440"
     CDS             complement(3323..4246)
                     /locus_tag="J8V14_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA0922519.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4467..6959)
                     /locus_tag="J8V14_002441"
     CDS             complement(4467..6959)
                     /locus_tag="J8V14_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBA0922520.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6966..7433)
                     /locus_tag="J8V14_002442"
     CDS             complement(6966..7433)
                     /locus_tag="J8V14_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBA0922521.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7569..7642)
                     /locus_tag="J8V14_002443"
     tRNA            complement(7569..7642)
                     /locus_tag="J8V14_002443"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7606..7608),aa:Arg,seq:acg)
     gene            complement(7700..7773)
                     /locus_tag="J8V14_002444"
     tRNA            complement(7700..7773)
                     /locus_tag="J8V14_002444"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7737..7739),aa:Pro,seq:tgg)
BASE COUNT         2168 a         1696 c         1255 g         2673 t
ORIGIN      
        1 tggcttttga acaccgttta tcagaggttg taaaatcagt gttttgtcct gagtattaga
       61 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggac
      121 agctgattga acactatttg ttcggaaaaa gtgactaact atgacacaat ttttttcaca
      181 gtcccttttt tcacttgatt tgattcgtac cagcgaactt gacgagaact ttcttggttt
      241 ggtaactata tggtattctt ttaacatcct cgtttgcttc ttctaattta tttatacaaa
      301 gtcacacttt tttaaacatc tgttcttcga tacatttcta tttcttttaa acatcgatac
      361 caatattttg aacttaagag tgcataccaa atggattcat ttactcttcg tcagttacga
      421 agataatcaa aatctccaac ttccctactt atttcgtttt gagaattatc gaagcaatct
      481 tacataaata actaaaaaac aaaaaaccag aaaatcagta tactgatttt ctggttttac
      541 atggtttgtt caataaaaag ctgttggaac tgttaatatt aaattatcgc atcgttacga
      601 attcttccgc tcctgttgga tggatggcta ctgtattgtc aaagtcttct ttagttgcac
      661 ccatcttgat agccacggca aaaccttgca gcatctcatc gacaccaaca ccaatagcat
      721 gtaagccgac gattctttct tcttttccga cacagatcaa tttcatttcg catttttggc
      781 gataatcatt taatgcaaag tacattgggg taaagcgtga acgataaact ttcaattcat
      841 tttcgccata tttttccaat gcttcttctt ctgtcaaacc aattgttgca attggtggat
      901 gcgtaaagac gactgtcgga acaagtttat agtcaagata agaatctttc tttccattaa
      961 ataaacgttc tgataaacgt ctaccagctg cgattgcaac aggagtcaga tctaatttac
     1021 cgatcacatc accaacagca aaaatatgtt catccgttgt attttggtat ttatctactt
     1081 tgataaaccc ttttttgtcc aattccacat ttgtattttc cagacctaat ttttcggtat
     1141 tcggtacacg tccaccggca aaaagtacac aatcaccagt taaggtttca ccattttcaa
     1201 atagcaccgt atattcgtca ttgtttttag taatttcttt agcaatatga tgtgcgtaag
     1261 tttttattcc agcttcttgg tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg
     1321 ttctaagtgg tcgttctttt cgatatgccc acatgacttc actacctaaa ccacgaaata
     1381 cacctgaaag ttcagccgca atgtatccag cacctaaaac gattgctctc ttaggtaact
     1441 ctgttaattc aaaaaatcca tttgaatcaa ttgcgtattc tccaccagga atggtcattt
     1501 tacttgcacg gcctccagta gcgatcaaga ttttaggagc acgataggtt tcaccgttta
     1561 cttcgatcgt ttgactatcg ataaaagtag cgtaaccttt tatagcctcg acatgattgc
     1621 tatctaatcc tctttgataa gcaccgtgta gaaaatcaat atatttttct cgtctttcta
     1681 ctagaccggc gaaatcaaca ttttttaaat ctgcttggat accgtaactt gaggcatctc
     1741 gttcaattgt ttccaaaatc gtacttgctt gccacataac ttttttaggt acacaaccga
     1801 cgttgacaca tgttccgccc aattcattcg cttcaatcag caaaactttt gcgccatgca
     1861 tccctgctct gtttgctgaa gcaataccgc cagagccgcc accaattacg atgtaatcat
     1921 atatttgcat tttgttcctc cttattgaag agatcaatca aaacctttat ttaacgatcc
     1981 tttttctaca gaaaacaagt gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa
     2041 acagggatat atcagctttt tccttattta cgttttcgga tcatatctcc aggtttcacc
     2101 ggctgtgcga ctggcatagt taaaatcatc attggatttg gtgctcggtc aatggattcg
     2161 ccttcttcat tatgcataac ttcaacggtt tgactgaaat gactgaatcc tggaccataa
     2221 aattcgattt catctcctac agaaaacaag tttctctgac gtatcgtagc aatctgtgtt
     2281 gcctcatcat aagataatac ttcaccgaca aatttatata ctgggatttt acgacgttca
     2341 ccgaataatt gttcgttttc agttggtgta ttgtagtaaa aacctgtagc taattctcgt
     2401 tgtgctactt tccataactc gtcgatccat tcttgcttgc agacatagtt ttcaggatct
     2461 tccatataac tgtctacagc cgctttatag acatttgaca ccgtagatac ataatggatc
     2521 gatttcattc ttccttcgat cttgaagctg tctacgccgt tttcaatcaa ttctggaata
     2581 tgttcgatca ttgacatatc cactgcactc atcgagaatt cttcttccac ttcgcctgta
     2641 tcagttaaac tgtttcgttc ttgaccaaaa ggcatatcga acaatccata cttccagcga
     2701 caagattgag aacatccgcc acggtttgca tcccgcatcg acatatggtt ggacaatgtg
     2761 catcgaccag aataagagat acacatggca ccatggataa atgcttcgat ttcggtatcc
     2821 gtatttttac gaatctctgc cacttcctcc attgaaactt cacgagctaa aacgacacgt
     2881 tccaaccctt cttctttcca gaattccaat gtctcaaaat tagttgctga agcttgagtg
     2941 gataaatgga tcggcagacc tggggcttct gtcgcacaaa tttcgatcaa agctggatca
     3001 gaaacaatca ctgcagaaat accgacatca cgcaaactgc ggaaaaattc ccctgctcca
     3061 tcttcatttc cttcatgggt gaccatatta gcagcaacgt ataccttcgc atcatgttct
     3121 tttgcaaaag caactccttc agccatttct tcataagtaa aattacctgc acggcttctt
     3181 aaaccatatg cattcccgcc aatatatact gcatctgctc cataatggat cgcggttttt
     3241 aacttttcca gtgttcctgc tggcgctagg atttctggtc gtttcaattt tctttttact
     3301 gtcatttctt tcatcatact ccttatttga tagcagaagg atcaatataa aagaatcctg
     3361 tatctaattc acggttttca ggatgcaatt tactgatttc gctagtcatt ttttccgctt
     3421 tttcttgatc ccagctacct tctaaaattt gtttttttgc ttcggcgaat agtcgagtga
     3481 tgtttacgaa tttttcgcct ggagtaaata atccgtccaa tttccatgta cagtaatgat
     3541 gctgataaag ttcattcaat tcattcgaga gattcagatc attgctggca aaaatatgtg
     3601 tgccatgact atcttcataa atagaataat gtgtctcttc ttttttaggt tccgagataa
     3661 aaagacctct ttcacgatct ttttgttcgt cttgtttcgt atagttatag taattctgta
     3721 gcagtggacg tttggattga tggatgcatg tagcaccata cacaagggtt tctacaggaa
     3781 tatcgagttt ttcttccatc gccttcatct cttcaaaagg aacttcacga gcaagtactg
     3841 ctccaactgc gccttttttc gcccagaaat tgatttgacg tgcgctcgtt actaatgttt
     3901 cagcatcata aatatacggt atacgcaatt caggattttt tttcatcgta taaataatac
     3961 ctgtatcacc aactgtgatc acatctacgt ttatttcttt taaaaaagcc aaatattcag
     4021 gtacattttt cattttttca ggatgaatga tcccatttac agcaatattc gcttttttac
     4081 catacttgtg tgctaattct accaattgtc gctgttcttc tcgtgtaaat gaagcaggca
     4141 gacgcaatcc aaattgctct tcaccgaaat atagtgtatc tgcacctgca gcaagcaact
     4201 gttctgcttg ttcaatagtt tctactgtgg ttatgagttc gatcataatt ttctcctcct
     4261 acaacttcat aatagtagca tagctctaaa aactttccaa tacttcttct atttaaagtc
     4321 ggatttttga aaattatttc acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat
     4381 ataaaaagag attgtgacaa aaatcttgtc acaacctctt aatcaagtct tactattata
     4441 aaaattcagc tacacaggcc aagtaactat agggaaccta ctgtttcctt tttaggtccg
     4501 cgaacattca atgtgatctt accttttgaa gcacctactg ttacgtggtc acctaaacga
     4561 atttctccgc caagcaaagc ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga
     4621 cggattggac gtgcaccata ttcaggatca aatccagctt tcccgatcac atcgattgca
     4681 gatggtgtga ttttcacttg aatatcttgt tctttcaatc gtttgataat cgctttgctc
     4741 atgatttgga cgattgtatg gatttcatct ttactcaatg aatggaagac gacagtctca
     4801 tcgattcgat tcaagaattc aggtcggaaa gctttcttta actcttctaa gatacgtttt
     4861 tgcatggctt tgtgatcttt agtaacatct tggacattga atccgacatt tttttcttct
     4921 cgaatttgtg tcgctccgat atttgaagtc ataatcatga ttgtatttct aaagtccact
     4981 tttctacctt tagaatctgt taaatgacca tcatccaata cttgcagaag taaattgaaa
     5041 acgtctggat gtgctttctc cacttcatct aagagaatca ctgaatatgg tttttgacga
     5101 attttttctg tcaactgtcc accttcatca taccctacgt atcctggagg agacccgatt
     5161 aatcggcttg tactgtattt ttccataaat tctgacatat caacacggat cagggcatct
     5221 tcacttccaa acatgacttc tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt
     5281 ggtccaagga acatgaatga accaattgga cgatcaggat ctttcaatcc gcttcgtgca
     5341 cggcggatcg cacgtgaaac tgctttgaca gcctcatctt gaccaacgac acgttcatgc
     5401 agaagtcctt ccagttctaa caagcgttcg ctttcttttt tctccaactg ctgtagaggg
     5461 acaccagtcc actccgatac aactgtagca acatcttctt ctgttacacg atcagaataa
     5521 ccttgctcct gttttgcttc aattgcagaa actttttgca gctttttacg tagtgctttt
     5581 tcttgtctgc gtaattgagc agcattttca aagtcttgtt tttggatcgc tgcttccttt
     5641 tcttgtacta gttcatcgat ttctagtttg atcacagttg attttgttaa gtgatcggtt
     5701 tgatccaaac ggactttcgc tgctgattca tcgatcaaat caattgcttt atcgggtagt
     5761 tgacgagaag tgatataacg aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg
     5821 atctccactc catggtgctc ttcatatctt gtacgtaatc cttgaagaat cacttctgct
     5881 tcttcaggag ttggctcatc tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct
     5941 ttttcgatat atttttgata ttcatctagg gtcgttgctc cgatcgtttg taattctcct
     6001 cgagctaacg ccggtttcaa gatattcgaa gcatcaattg ctccttctgc tccacctgca
     6061 ccaattaagg tatgcaattc atcaatgaat aagattactt gaccatcatt atagatttcg
     6121 tcgatgacct ttttcattcg atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa
     6181 gatcccatat caagcatcat caggcgcttt tcttgcatgt cttctggcac ttcacctaaa
     6241 atgatttttt gagctaaccc ttcagcaatg gctgttttgc ctacaccagg ttcacccacc
     6301 agtacagggt tattctttgt ccgtcggcta agaatctgga taagccgttt cacttctctc
     6361 gaacgaccaa caactgggtc cagcttgttt tcttttgcta atttcgtcaa atcacgtgct
     6421 aatgaatcta gcgtaggtgt tccttgcggt gtttgccgtt tatcttgtac atttacattt
     6481 ctacgacgat tcattccttt tccagcagaa tcaccagcta gccccatttt ttgtttcaga
     6541 acttgacggg cttttgaaag gctgatacct aagttgatca tgattcgagt agccatgata
     6601 ctttcatctt ttaataagct catcaaaata tgttctgttc caactttttg agaaccagtt
     6661 ttcttcactt cgctgctagc taaagcaaaa acttgtttca ttcttggtga atatggaaga
     6721 aagactcctt tagggtaaga acggatacca ccataaccaa tcagatgttc aatttcttca
     6781 taaatttccg tttcattcaa atccatttca cgtaatactt ttccagcaat tccattttgc
     6841 tcgaccacta aggctagtaa aacatgttct gaactaactg attgatgttt caaccgtttt
     6901 gcttcttctt gtgcaatttc taatactgct tgggctcttt cagtaaaaag ttcttccatc
     6961 taatctcact tcccatcttc ataacttaaa cgttccagaa atgctcggag aatccgagca
     7021 cgtgtatgat cttcatattc attgaaattc aatgtgtttt ttgcaattgc actcaacatc
     7081 aagtttcctt ctttttttgt aattatttgg tcttcataaa gtttttgaat aatagcaaaa
     7141 gcatttttct cactgattgt atcatcaaat aactgattta tctgctcaag taattgtttt
     7201 ttatctgaaa tccttacttt tgcaattcgt atataaccac caccaccacg tttgctttct
     7261 actagataac cacgttggat ggtaaaacgg gtattaatca catagttgat ttgtgaagga
     7321 acacagttaa agaggtctgc catttcaatc cttcgaatct cgatcatttc actattttcc
     7381 agaattttct ttatatatga ttcaattaaa tccgaggtat tctgatgtgc catcttattc
     7441 attccctttg actaatattg accttaatta taacgaattt gataatagaa agaaagaaga
     7501 acgctttttg ttaaataaat cctttagaat caacaaaaaa aaatacggaa tatactattc
     7561 cgtatcgacg cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc
     7621 tatccagttg agctatgggc gcatattcaa tttaaaaaaa ttataaatca tttgaaaaca
     7681 aaaagcaaga taaaaaaaat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa
     7741 accaagtgct ctaccaagct gagctacttc ccgtttaaaa aataaaaaag ac
//
LOCUS       DADQRX010000112         7755 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.117, whole genome shotgun sequence.
ACCESSION   DADQRX010000112 DADQRX010000000
VERSION     DADQRX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.117"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="J8V14_002445"
     CDS             133..3660
                     /locus_tag="J8V14_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBA0922522.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="J8V14_002446"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="J8V14_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBA0922523.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..>7755
                     /locus_tag="J8V14_002447"
     CDS             7448..>7755
                     /locus_tag="J8V14_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922524.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     "
BASE COUNT         2674 a         1331 c         1631 g         2119 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggag
//
LOCUS       DADQRX010000113         7677 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.118, whole genome shotgun sequence.
ACCESSION   DADQRX010000113 DADQRX010000000
VERSION     DADQRX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.118"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..2758)
                     /locus_tag="J8V14_002448"
     CDS             complement(56..2758)
                     /locus_tag="J8V14_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBA0922525.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2771..4060)
                     /locus_tag="J8V14_002449"
     CDS             complement(2771..4060)
                     /locus_tag="J8V14_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBA0922526.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4210..5256
                     /locus_tag="J8V14_002450"
     CDS             4210..5256
                     /locus_tag="J8V14_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922527.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5258..7411
                     /locus_tag="J8V14_002451"
     CDS             5258..7411
                     /locus_tag="J8V14_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBA0922528.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2158 a         1570 c         1401 g         2548 t
ORIGIN      
        1 tctaaaattt ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt
       61 ccgccataca ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta
      121 ttgggacaac cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat
      181 tgtctttagt tatttccaat ttttctaaat gactagacat attgaaacct cttactacta
      241 ctttatcact aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg
      301 ttaaatattg ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa
      361 aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta
      421 gttcaatgct gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat
      481 atccccaatc gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt
      541 ctgttacttc atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat
      601 gaatattgac aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca
      661 catggtttgg gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta
      721 actttgtagg aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt
      781 caaagtcgat ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc
      841 gtttttcttt ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt
      901 tttctaaacg ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg
      961 ctatttccgg tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga
     1021 ttggctgatc acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt
     1081 caaaaatatg cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac
     1141 catttttttc aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa
     1201 tcattgtttc cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag
     1261 aaaatgctgg catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca
     1321 cacggaaacg gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag
     1381 ctggtatagt ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta
     1441 actcttgata tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg
     1501 ttatttctgc ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc
     1561 tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg
     1621 caagatactt tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag
     1681 aatcgactga acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag
     1741 cataatctaa ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg
     1801 gttctgtaat attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc
     1861 gagcagatgc tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc
     1921 cttctactga gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa
     1981 aattagaatg aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg
     2041 atatatcttt ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag
     2101 aggcatattg ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt
     2161 ctactggaat ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg
     2221 tttgatcagg tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca
     2281 aaacttgatt atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac
     2341 cagcttgttt cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc
     2401 ctaacatgac aggggttccc catttacggc tttcttccat gccaatcagc atatttctca
     2461 cgttcgattc cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca
     2521 attttccttc attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat
     2581 agtcatctaa aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt
     2641 caaatagctc tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag
     2701 ccatgtacca ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt
     2761 acgatacctc ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca
     2821 tcatatttgc ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc
     2881 cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt
     2941 tttcagattt gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt
     3001 tttctggcgt atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat
     3061 tttctttact caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat
     3121 atcccaagta aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt
     3181 ttcccattcc atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc
     3241 cgtgattctg aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt
     3301 ctttatcatc gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa
     3361 acatattgga tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag
     3421 accatgtcat tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt
     3481 cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt
     3541 tgaacacgtc caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt
     3601 ttcctgtatt tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt
     3661 catattttct ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac
     3721 cgttagcttc ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct
     3781 ttttgactaa cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat
     3841 aaggacgaac ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata
     3901 aaaaggtggt tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg
     3961 ctgtaaaatt ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca
     4021 taaatttctg caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat
     4081 ttgatatatc tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat
     4141 atctttttat ttactttttc atccattatg gtattatttg aatatgatat atcattaaga
     4201 ggtgtttata tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt
     4261 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
     4321 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
     4381 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
     4441 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
     4501 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
     4561 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
     4621 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
     4681 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
     4741 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
     4801 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
     4861 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
     4921 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
     4981 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
     5041 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
     5101 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
     5161 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     5221 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     5281 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     5341 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     5401 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     5461 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     5521 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     5581 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     5641 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     5701 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     5761 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     5821 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     5881 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     5941 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     6001 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     6061 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     6121 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     6181 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     6241 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     6301 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     6361 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     6421 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     6481 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     6541 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     6601 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     6661 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     6721 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     6781 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     6841 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     6901 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     6961 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     7021 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     7081 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     7141 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     7201 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     7261 caaacggatg gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     7321 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     7381 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     7441 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     7501 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     7561 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     7621 taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctg
//
LOCUS       DADQRX010000114         7673 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.119, whole genome shotgun sequence.
ACCESSION   DADQRX010000114 DADQRX010000000
VERSION     DADQRX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.119"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="J8V14_002452"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="J8V14_002452"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBA0922529.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1897..2649)
                     /locus_tag="J8V14_002453"
     CDS             complement(1897..2649)
                     /locus_tag="J8V14_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0922530.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(3013..5679)
                     /locus_tag="J8V14_002454"
     CDS             complement(3013..5679)
                     /locus_tag="J8V14_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBA0922531.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6020..7471
                     /gene="cls"
                     /locus_tag="J8V14_002455"
     CDS             6020..7471
                     /gene="cls"
                     /locus_tag="J8V14_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBA0922532.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2094 a         1679 c         1364 g         2536 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca tcataaaaag aacccttcac gagtcgtgaa aggttctttt
     1921 tttatctgcg gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg
     1981 tttttcactg aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc
     2041 atagtatagc ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt
     2101 tttcttcact ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg
     2161 cgatgtatta ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc
     2221 atcgattgat tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt
     2281 ttctaattga ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt
     2341 ctcatttgat tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc
     2401 atttttttct gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc
     2461 atagcgatta atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc
     2521 tgctgtttca gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat
     2581 agttacttgc gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt
     2641 ttcattcatt tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca
     2701 ttctcctctt ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc
     2761 tatcataaat aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata
     2821 aacagtgtcc aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt
     2881 ccttaaacgg tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg
     2941 agaagctgtt tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct
     3001 cgttgattcg ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat
     3061 caaaacaatt gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc
     3121 tgatgggata gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct
     3181 acatacaact acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt
     3241 ttgtgtgttt gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa
     3301 tgacatggct acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac
     3361 agcaatccca atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat
     3421 tcctgaattt ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc
     3481 taatgcaatt gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt
     3541 aaatggattg gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt
     3601 tcctgcaaat agataagaaa tagctttctt gatattatca aacacattac gtcctacttc
     3661 tactgcgctg acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt
     3721 tgccacatct gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc
     3781 gtcatttaca ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac
     3841 gatacgaatt ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc
     3901 gtttagttga tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc
     3961 ttcaaaaatc ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat
     4021 catgattgtt ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg
     4081 tggaggatcg atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga
     4141 taaatcaaaa ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga
     4201 aaaagcttca ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc
     4261 accatttagc aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa
     4321 caagtaattt ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa
     4381 aggaagttct gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt
     4441 ttcactaaag tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact
     4501 gatcgatgca tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca
     4561 ttttgaaggg tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac
     4621 tgtcattttg ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc
     4681 aagtgtttca actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt
     4741 tcccaaagat aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc
     4801 tacggctacg gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc
     4861 tccaagataa atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc
     4921 caaacttagt tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt
     4981 tgttgattcc aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac
     5041 taagaattta ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc
     5101 tccgattggt accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc
     5161 tgtcagcata ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg
     5221 tacataatcg cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat
     5281 cgttttttcc ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc
     5341 ttctaaagaa ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa
     5401 tactgcaaaa ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac
     5461 aatcagcaat acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg
     5521 cttcttcggc ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg
     5581 cgtggtgctc aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc
     5641 tttatagacc gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga
     5701 tcttggccct agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag
     5761 gtctcaccat ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg
     5821 caacaaacgt tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt
     5881 attaacctac aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg
     5941 aaaagtatat tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta
     6001 tgtaaggagg aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc
     6061 acttattgct ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg
     6121 gctcttagta ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg
     6181 tggattaacg gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa
     6241 ctttcagtct tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga
     6301 caaacagcaa tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa
     6361 ttctgtcact cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa
     6421 aaaagccaaa cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca
     6481 gcaagtctta catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata
     6541 tgatgcattt ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg
     6601 tggacatgtc catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta
     6661 ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa
     6721 tattgccaat caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat
     6781 acggattttc ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt
     6841 ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga
     6901 tcgagatgaa tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa
     6961 cgaaagggaa caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt
     7021 ttggatacag acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc
     7081 aactgcctct ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat
     7141 ttatcgagca acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt
     7201 ctatgagaac ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt
     7261 aggttcagca aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat
     7321 ttacgatcct gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg
     7381 ttcatccatg acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca
     7441 aatttctcga ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac
     7501 taagattaaa gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg
     7561 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
     7621 ttgaacttgt tccttgtgac tcggagctgg acggcaggct cacgacctct ttc
//
LOCUS       DADQRX010000115         7599 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.120, whole genome shotgun sequence.
ACCESSION   DADQRX010000115 DADQRX010000000
VERSION     DADQRX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.120"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..426)
                     /locus_tag="J8V14_002456"
     CDS             complement(136..426)
                     /locus_tag="J8V14_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922533.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="J8V14_002457"
     CDS             complement(544..804)
                     /locus_tag="J8V14_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922534.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="J8V14_002458"
     CDS             complement(941..1354)
                     /locus_tag="J8V14_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBA0922535.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="J8V14_002459"
     CDS             complement(1810..2295)
                     /locus_tag="J8V14_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922536.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="J8V14_002460"
     CDS             complement(2434..2649)
                     /locus_tag="J8V14_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBA0922537.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="J8V14_002461"
     CDS             2873..3673
                     /locus_tag="J8V14_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0922538.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="J8V14_002462"
     CDS             3692..5560
                     /locus_tag="J8V14_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBA0922539.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="J8V14_002463"
     CDS             5553..6044
                     /locus_tag="J8V14_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBA0922540.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="J8V14_002464"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="J8V14_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBA0922541.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="J8V14_002465"
     CDS             6604..7599
                     /locus_tag="J8V14_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBA0922542.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DADQRX010000116         7474 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.121, whole genome shotgun sequence.
ACCESSION   DADQRX010000116 DADQRX010000000
VERSION     DADQRX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.121"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            275..1753
                     /locus_tag="J8V14_002466"
     CDS             275..1753
                     /locus_tag="J8V14_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBA0922543.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="J8V14_002467"
     CDS             2030..4090
                     /locus_tag="J8V14_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922544.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="J8V14_002468"
     CDS             4173..4742
                     /locus_tag="J8V14_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA0922545.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="J8V14_002469"
     CDS             complement(4917..5723)
                     /locus_tag="J8V14_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922546.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="J8V14_002470"
     CDS             5861..7375
                     /locus_tag="J8V14_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBA0922547.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2683 a         1226 c         1459 g         2106 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaag
//
LOCUS       DADQRX010000117         7388 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.122, whole genome shotgun sequence.
ACCESSION   DADQRX010000117 DADQRX010000000
VERSION     DADQRX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.122"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..1833
                     /locus_tag="J8V14_002471"
     CDS             202..1833
                     /locus_tag="J8V14_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA0922548.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1836..2915
                     /gene="uxuA"
                     /locus_tag="J8V14_002472"
     CDS             1836..2915
                     /gene="uxuA"
                     /locus_tag="J8V14_002472"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBA0922549.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2989..4416
                     /gene="uxaC"
                     /locus_tag="J8V14_002473"
     CDS             2989..4416
                     /gene="uxaC"
                     /locus_tag="J8V14_002473"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBA0922550.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4500..5147
                     /gene="eda"
                     /locus_tag="J8V14_002474"
     CDS             4500..5147
                     /gene="eda"
                     /locus_tag="J8V14_002474"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBA0922551.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5267..6643
                     /locus_tag="J8V14_002475"
     CDS             5267..6643
                     /locus_tag="J8V14_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0922552.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6653..>7388
                     /locus_tag="J8V14_002476"
     CDS             6653..>7388
                     /locus_tag="J8V14_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBA0922553.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGD"
BASE COUNT         2556 a          962 c         1508 g         2362 t
ORIGIN      
        1 tacatgaaaa aaagaaaaaa taagtttcga taaaatcagt ccaaaaagaa ggagtatttt
       61 atttttctga ataaaaaata caatcattct gatatggtag aaaaaagcaa gtttattccc
      121 tttttatatt gacaacggtt acaatgggtt ttaaaatata aaatgtatca aaactaacat
      181 atcagtttga ggaggttaat tatggtttcg attaaaaatt ggcaaaattc tatcgaagaa
      241 ttcaatgaaa tgcagattct actgccgtgt tttgatgtca aagaagttaa gcaagaaggc
      301 cagcaacatc cagtatggct tcattttggt ggaggaaatc tttatcgagg atttcatgca
      361 gaaattgctc aatgcttagc agatcaaaat gaattagaaa caggagtagt tgtgtgtgaa
      421 acatttgatg aagaagtagt ggaaaaagct tatcatgact ataacaatga cattttggaa
      481 attgttatga gtggagatgg tgaactcaaa aaaagacttt tggcagtaac ggctgatagt
      541 ttatattgca atccgaagaa tatagcggac tatcaaagag tggttaaata ttttgaagat
      601 cctaaattac agtttacaac gtttactatt actgaaaaag gttacagttt aaaagattct
      661 aaagggcaat tcttttcggt agtcaaagct gatatcaaat ctggacctag caatcctcaa
      721 catacgatga gtattgtaac atccttgtta tatagcagat ttaatgctgg agagttgccg
      781 attgcgatgg tttctactga taatttctca cagaatggtc aacgttttca agatagtatt
      841 tttacgattg cagaagcttg gcaaaaagaa ggatatgttt ctgaaaaatt tattgcttat
      901 ttaaaggata aagaaaaagt gacttttcct tggagtatga ttgatcgaat tacgccaaat
      961 ccttcagaga atgtaaagga agatctattg atgatggggc tcgaggattt agatatcatt
     1021 cacacaaaaa aacataccaa tattgctgct ttcactaata ctgaggtagt tcattattta
     1081 gttattgaag atagttttcc aaatggccgg cctaaattgg aaaaggctgg tgtgattatg
     1141 acagatagag agacggttga taaagcagac atcatgaagg ttacgacaag cttgaatcca
     1201 cttcatactg ctttggcagt aacaggttgt ctgctaggat atcaatcaat ttcagcagag
     1261 atgcaggatg atgatttggt tggtttaatc aaaggaatag gttatttaga aggtttacca
     1321 gtagttgaaa atccaggcat tattgatcca aaacaattta ttgatgaagt aattaataaa
     1381 cgtttgccta atccctatat tccagataca ccacaacgaa ttgcaacaga tacttctcaa
     1441 aaagtagcga ttcgctatgg tgaaacaatt aaaaagtatg tcgaaaaaga cggaaatgca
     1501 gaaaatctaa catttattcc tatcgctatt gctgcttggt tacgttattt actggcagtc
     1561 gatgatcaag gggaaaattt tgtgccaagt cctgatccgt tattggtaga gttgcaggat
     1621 aaattatcag tattgaaatt aggtgaacaa gatccttcaa agattcattg tgcggtacag
     1681 gaaattttag aaaataaaga aatctttggt ttagatttat atcaagttgg ggtaggaaag
     1741 aaaactgaaa aaatcttttg cgaaatgcta gtaggtcctc aagcagttcg taaaacaatc
     1801 aataaatata taacagaagt tggaggaaag taactatgga aatgactttt agatggtatg
     1861 gggcaaatga agaaaaaatc tcactacaag aaattcgtca gatcccagca atgaaaggaa
     1921 ttgttggaac attaatggat ataccagccg gagaggtttg gccgagagaa cgaattaaat
     1981 ctttaaaaga tgaagtcgaa gccaatggac ttaccttgaa agttattgag agtgttaatg
     2041 ttcatgatga tatcaaaatt ggtttaccaa cacgagatca atacattgag aattataaac
     2101 agacgattcg caatttggca gaatttggga ttgaggttat ctgttataat tttatgccag
     2161 tatttgattg ggtaaaatcg gatttggatt atcgtttaga tgatgggtct tctacgttag
     2221 cctatatttc aaaaaatatc cctactgatc cggaggaaat tattagagaa gtagaaaatt
     2281 ctactaataa ttttgaattg ccgggttggg aacctcatcg gttatcacaa attaagcaat
     2341 tattagaagc ctataaagaa gttgatgaag agaaattacg agagaacttt gcatattttc
     2401 tcaaagcaat tatcccgaca tgtgaagaag ttgggattaa aatggcagtt cacccagatg
     2461 atccaccata ttccattttc ggattacctc gtattgttaa aaatcgtgac gatttagatt
     2521 ggttgtgtaa tgtagtggat tctccttcta atggtattac attatgtact ggaagtattg
     2581 cagaacgacc agataatgat gtctatgaaa ttctatctga attttgtcaa cgggatcgga
     2641 ttccttttgc acatgttcgt aatatcaaat atttaagtga caatggactc gatttttacg
     2701 aagcgccgca taaatcagaa tatggttctc tagatatgta taagatatta aaaacaatgt
     2761 acgataatgg atttaatggc tatattcgtc cagatcatgg tcggatgatt tggggagaaa
     2821 ccggtagacc tggttatgga ttatatgacc gagctctagg tgcagtttat ttaaatggta
     2881 tttgggaagc gcttgaaaag ggggcgagta aataattata taagggagtt tatttagagg
     2941 aaaaattttc tcttctaaaa actaacggaa ggaaaggagt gtttaaatat gtttttagat
     3001 aagaactttt tattagagaa tgaaatagct aaaagacttt ttcataaatt tgcaaaacac
     3061 caaccaatta tcgactatca ttgtcattta gacccagcat atttgctaga aaataagcaa
     3121 tacaagaatt tgactcaggt atggttaaat gataaaggag ctggagatca ttataaatgg
     3181 cgattgatgc gagctaatgg tatagaagaa gagttgatca gtggtccagg tgatgattat
     3241 gaaaaatttt tagcttttgt aaaaacagtg gagaaggcgc caggtaatcc tatttttgaa
     3301 tggtctcatt tggaattaca acgttacttt gatattgatt tgacaatttc tgagaagaat
     3361 gcaccagaaa tttggcgacg tgctaacgaa aaaattcaaa cggattccta tcgcccgaaa
     3421 gaattgttgg agatgatgga tgtgaaagcc atctgtacaa cggatgatcc tacagatgac
     3481 ttgtggtatc atactgagct ttctaaacag gataattctt tcaaggtatt acctactttc
     3541 cgtccagata aggtctggag tatcaaatca tcaggctttt cgaactatat tttaaaactt
     3601 agtcaaacag cggctatacc aatcaaagac tttaatgggt tgaagcaagc attgagccaa
     3661 cgagtggatc attttcatca aattggctgt cgattagcag atcacggttt gaatacttat
     3721 ttttatgaaa tagcagatga aaaaatactc gacaatctgt taataaaagc tttagcagga
     3781 aaaacggatt tccatttatc agaaattgca caatttgtta caggtattca attacatttg
     3841 atgtctgagt atacaagata tggttggacg atgcaaatgc atttaaatgc tttaagaaat
     3901 aattcaagta tattaaagga acaaattgga gtagatgtcg gtggtgattc gatgggggat
     3961 caagctagtt tgacacaaaa tgttgtagca ttgttcgata ccgctgaaaa gagtgggtgc
     4021 ttaccaaaaa caattttata ttcattaaat ccaactgatt ggcttccact agcaacagca
     4081 atgcaaagct tccaaggggg tatgattcaa aaaatccaat tgggatgtgc ttggtggttc
     4141 aatgatacag ctgaaggaat gcgtcaacag ttgactacga tggcacaaca aagtttgttg
     4201 tcgaatttta caggtatgtt gactgattct cgaagttttc taagctatcc tcgccatgag
     4261 tattttcgtc gagtattatg tcaattacta agcgagtggg ttaaagcagg ccgaattcca
     4321 gatgatgaaa tgtatcttag tcagatagtg gagaatattt gttatcgtaa tgcgtataat
     4381 tattttaatt tttttgaaat cggaagtaga aaataatact tgaaataaaa gcttaaattt
     4441 attctttgta agttagtaca gctagacaaa gtatgtatcc ataaaggaag gagatagata
     4501 tgaaaaaagt agaaatttta agtaagttag aagcggctgg agtcattgcg gttgtacgtg
     4561 ggaaaaataa ggatgaggcc attaaagcaa taaattcaat tattgcgggt ggaattaaag
     4621 ggattgaact aacttttaca gttcctaatg ctgaagaaat aatcaaagaa attgctaaaa
     4681 aatatgctga tcaaaaagat gtaataatcg gagccggtac ggttttagat gcgtttactg
     4741 ctcgtatcgc aattatggct ggggcagaat atatcgttag tccatctttt gatcgacaaa
     4801 cagctgagat ttgtaattta tatcaaattc cttatttacc aggttgtatg acaattactg
     4861 agataaagga ggcacttact gctggcgtag atatcattaa actattccca ggttctgctt
     4921 ttggtccatc cattgtaaaa gcctatcaag ctccattacc acacgtaaat ataatgccta
     4981 ctggcggagt aagtatagag aacttagaag attggtttga agtaggagtt gttgcagttg
     5041 gtgtaggtgg gaatttattg gcttctgttg aaaaaggtga ttttgaagaa gtaagtcgag
     5101 tagctagtaa atatatagat aagttttatg agataaagaa ttattaggag aaaaaacaaa
     5161 taatactctg aaatgtaaaa aataaatact gactctttac gcttcagagt atagtacgta
     5221 ctttcatcag aatgaaaacg cttaacaaca gaagggagag aattgtatgg aaagaaaatt
     5281 tggtataaga gacaaaatag gatatatgtt tggagatttt ggaaacgatt ttactttcat
     5341 ttttgctagt tcttttttaa tgatctttta tacaaaagta ttggggataa gcggagctgc
     5401 cgttggaaca ttatttttag tagcaagatt tatcgatgca gttactgatg taactatggg
     5461 gcgtattgtt gatagatcga aagcaactaa gcagggtaag tttaaaccgt ggattttaag
     5521 aatgagtggt ccagtcgctt tagcaagttt tttaatgtat caaacttcta tgatttcagc
     5581 ttctatgagc gttaaggtag tatacatgta tgttacgtat atattatggg ggagtatttt
     5641 ttacacatct ataaatattc cttatggatc aatggcatca gctattactt ctattacaga
     5701 agaaagaaca atgctatcga cattccgaaa tgttggagct actttagcaa gtttggtcat
     5761 tggtgtatta acaccattat ttatttatac aagagatgat tcaggcgctc aaatagttcg
     5821 tggtggatca acttttacaa ttgttgcggg aatattttcg atatgtgcac ttatttgtta
     5881 tttaatatgt tataaactaa ctactgaacg agtaagaatt gaccaatctc aatacaaagt
     5941 ttcattgtta aaatctttaa aagggttagt taaaaatagg gcattacttt ctataattgg
     6001 tgcggctatt ttcttactcc ttgctcaatt attaattatg agtatgaata attatgtatt
     6061 tcctgattac tataatgatg cacagggaat agttttaatg aattttatca atcctatttt
     6121 ggttttaata gttgtttcgc ctttaacttt gtttttaagt aaaaaatatg gaaaaaaaga
     6181 actggcatca gtaggaatgt tcttttcagc aatagtatat ttgatcttat ttttgataaa
     6241 agctagtaat atgtatgtat tcttaattct tagttcggtt ggctatatgg gtttgggtgt
     6301 gtttaatact gttatttggg caaatattac tgatgttatt gatgatcaag aagtaaagtc
     6361 tgatcaaaga gaagatggga ccgtatatgc ggtatattct tttgctagaa aaataggtca
     6421 agctctagct ggaggcgtcg gaggttggac tctttctatt ataggctatg actctttagc
     6481 aaatgtacaa acgggagatg ttttagaaaa gttatataac gcttcaacat taattccatc
     6541 aatttgtttt ctgattgtag gattaatttt atttttcctt tatccattat ctaaagataa
     6601 agtcttagaa aattgcgagc ttttaaaaca aaaacataaa taggagagaa atatgaataa
     6661 ttggatattt tgtattgatt cagatggctg tgttatggat acgatgactt ataaacacaa
     6721 tctctttttc ggaccaattg ctgcagagat ttttgatgtt catcatagag aaaaattcct
     6781 acaggattgg aataatatta atttatattc agagactcgt ggaataaaca gatttttagg
     6841 tttagttaaa acattagaaa aaaatgatgt aacatcgatt aatcgattaa agcactgggt
     6901 atctctaaca aaagaattgt ctaatccatc tttgaagagg gaaatagaat attctcagtc
     6961 agaggattta ttaaaagctt taaaatggtc ggaagaagtc aatcaacaga ttagtcaagc
     7021 aaaaggatta gataaaccct ttttaagtgc tagagatact ataaaagctc tgaaaaaata
     7081 tgggaagata attattgtaa gctcagctaa taaggaagct gttcaagaag aatgggaaag
     7141 acatgaatta ttatcattag ttgatgaact gtgttgtcaa gataaaggaa aaaaagaaga
     7201 tattattcga agtgtaattg aaaatggttg tgatttggat aagatattaa tgattggtga
     7261 ttctcctggt gatctagaag ctgcaaataa aaataaagtt ttcttttatc ctattttggt
     7321 caataaagaa aaagaatcat gggaaaactt aagaactgat gttttattta aatgtttatc
     7381 gggagatt
//
LOCUS       DADQRX010000118         7240 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.123, whole genome shotgun sequence.
ACCESSION   DADQRX010000118 DADQRX010000000
VERSION     DADQRX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.123"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..1130
                     /gene="truB"
                     /locus_tag="J8V14_002477"
     CDS             207..1130
                     /gene="truB"
                     /locus_tag="J8V14_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBA0922554.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1134..2075
                     /gene="ribF"
                     /locus_tag="J8V14_002478"
     CDS             1134..2075
                     /gene="ribF"
                     /locus_tag="J8V14_002478"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBA0922555.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2427..3212
                     /locus_tag="J8V14_002479"
     CDS             2427..3212
                     /locus_tag="J8V14_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0922556.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3225..4076
                     /locus_tag="J8V14_002480"
     CDS             3225..4076
                     /locus_tag="J8V14_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBA0922557.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4200..4652)
                     /locus_tag="J8V14_002481"
     CDS             complement(4200..4652)
                     /locus_tag="J8V14_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0922558.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(5117..5485)
                     /locus_tag="J8V14_002482"
     CDS             complement(5117..5485)
                     /locus_tag="J8V14_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HBA0922559.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5635..6117
                     /locus_tag="J8V14_002483"
     CDS             5635..6117
                     /locus_tag="J8V14_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBA0922560.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6154..7164
                     /locus_tag="J8V14_002484"
     CDS             6154..7164
                     /locus_tag="J8V14_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBA0922561.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         2451 a         1158 c         1514 g         2117 t
ORIGIN      
        1 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaagcataac
       61 atgatttcta tttacgtgag catgaatttt gttacgtttt tattgccgaa aaataaaaac
      121 cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat aattcagtta
      181 aatcaaaaat gaaatggaga aaagacatgg acgggttatt acctttatgg aaagaacgcg
      241 gaatgacaag tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg
      301 gacatggggg tacgcttgac ccagacgtag acggtgtgtt gcccatttgt attggaaaag
      361 ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc gagataactc
      421 taggtttcag tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag
      481 atatgtcttt atctgaaaaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca
      541 cccagatacc accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg
      601 cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc
      661 ggacaagtga attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag
      721 aatgcgggaa aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt
      781 accctgcgca catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg
      841 ctctgacatt agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc
      901 tgccaataga aaaaggagtg gaacggttca aacgagtaga tattactgat gaagtatggc
      961 agaaagtaaa aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt
     1021 cagaagaaat tgctttgttt tataaaggaa aagttgtcag tatttatcag ccaaacccga
     1081 aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata
     1141 tcatcaagat cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag
     1201 tgctgggatt tttcgacggc gtgcatatcg gacaccagaa agtcataaag acaggtatgg
     1261 aaattgcaca aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcta
     1321 ttgtctttaa aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag
     1381 aagaaaaaat ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt
     1441 ttgctcattt agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat
     1501 atgctgtttc cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt
     1561 ttcctgaata tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag
     1621 gtgaaaaaat cagctctacc cgtattcgtg gattactaga cagcggacaa gtaaaagaag
     1681 cagcaaaact attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac
     1741 gaggcagact actaggattc cctacagcaa atgtcaaagt aaagagcacg gttcatttgc
     1801 ctaaagaagg tgtttatgtg tcggaaataa aaattgggga aacatggtat ccagccatgg
     1861 gttcaattgg acataatgat acattcggtg aaggacggga gctgactgta gagttgtata
     1921 ttttggactt taatcaagat atctatggtg aacatgtcgc ggtacgctgg aatcaatttt
     1981 taagagatca agtgaaattt tctagtgtag aggaattgat tgaacaactc aaggaggatg
     2041 aacaagcaac agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt
     2101 tatgtataac tagcaattac aataaaatgg gtgataaata agcccaggaa aggggctgtg
     2161 acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc
     2221 ggaaataagc cgaaatttct tgaagctgac cacttctgtc acaacctctt tctttataga
     2281 aataaaagga cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac
     2341 tatatatctt ggtgtacact cactttaagg cggaaataaa agttatccgt aataacaggt
     2401 ttaggtatga aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag
     2461 taactggtgg ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa
     2521 tgaatgtagt tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa
     2581 tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa
     2641 tacagcgttt acttgatacg gctttagaaa attttgggac actggacgtt tgggttaata
     2701 atgctgggat ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag
     2761 tgttaaaagt caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta
     2821 cagagaataa taaaaaagga aatatcatca atatgtcttc tgttcatgaa cagatcccat
     2881 ggccaacgtt tgctcattac gctgcttcaa aaggcggggt aaaattattt accgaaacgg
     2941 tagcaatgga gtatgcaccg aagggaatca gaatcaatag tataggccct ggtgctatcc
     3001 gtacacctat taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta
     3061 tgatcccact aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg
     3121 cttcagacga atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt
     3181 tatatccttc tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg
     3241 cattgatccc ggcagttggc tgggggatcc agccactgat tttgaaaaag attggtggca
     3301 gaccaacaaa tgagatatta ggaactggta ttggagcggt gcttgtcggc ttactcgttc
     3361 aattattcgc ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg
     3421 gtgcattttg ggttattgga caaacaggac agtatacatc tttcaacttg attggtgttt
     3481 ctaaaacgat gccaatttcg actgcattac agttagtcgg aacctcgttg attggtgtct
     3541 tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat tggagcagca gcaatcattc
     3601 ttcttgtatt tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta
     3661 tcacaaaagg aattagtcta ttagcatcaa caagtattgg ttattgggta tacagtgcat
     3721 tgcctaaact agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct
     3781 ttttaggggc agtgatttat gtgattttcc gtaaaccaac cgcattttct gaagacaaga
     3841 gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc agcattggct tatatttttt
     3901 cagcaaatga aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct
     3961 ctacattagg cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa
     4021 cgattatcgg attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat
     4081 aaagatggta aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt
     4141 ataaaaaaat agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt
     4201 taagatacaa gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag
     4261 tttacgacat aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga
     4321 tcgtattgta aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat
     4381 tgttctcgga attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg
     4441 attggtgcat ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg
     4501 tataggaact caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct
     4561 tttagttttc cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg
     4621 gctaattcat ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta
     4681 ctgacttagt tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc
     4741 attgtaaaaa tcaagtcaga aaattctaac ttttatttct ctaaagaaat tcgttctctc
     4801 ctaaagtgat attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac
     4861 tgatattttc tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat
     4921 aattttacta agtagaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa
     4981 gactccttat aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa
     5041 aggataggat tgaacaaaac ctgtataagg tgatgaatag tttcctcgcc agtaaaaggt
     5101 ttattcgtat aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa
     5161 atgaaatcaa ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga
     5221 cgttatccgt atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg
     5281 ctttttccgg tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac
     5341 catggtcagt aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt
     5401 ctgttatctt agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatttgat
     5461 tgatgatcgg ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt
     5521 taccagaaaa acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg
     5581 ctatatttat atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt
     5641 gacttggcaa agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc
     5701 atttatacaa atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag
     5761 accaaaagct tattcttaaa agataaaaaa gataataact acgtatatct gacttttact
     5821 acgaaacaaa cggatttcaa agcattaggg aagatcgtcg gtaaaaggct ttctattgtt
     5881 tcatcagaac gcatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt
     5941 tatgaaaaac agattccgat tattattgat gaagagctct tatcaaatga aaaacttgtt
     6001 tttgcaccag gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc
     6061 atccagctac ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca
     6121 agtaaaatag tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga
     6181 aatgcaacga ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta
     6241 ctcgaatcag ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg
     6301 ctttggacgc aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat
     6361 caaatgatgc aagatgaaaa tatccaaatg atctttccga cttggggagg agaattattg
     6421 attgagattc tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat
     6481 tcagatatta gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat
     6541 ggaactaacc ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa
     6601 agtgtactga aaacaagaga aggagaatcg attctacagc aatcttctga aaagtatcaa
     6661 aaagtgtggc cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat
     6721 tggcaatcag tcagcggaga tgcagtaagg atgaagggac gattattagg tggttgtatt
     6781 gatacgatca ggcatttaat cggtactcct tatggagatg tccaatcttt taaaagaaag
     6841 tataatgatg gaaagccgat cttgtggttt ctagaaaact gtgagctatc gtcagttgat
     6901 ttaagaagat cacttgttca gatgaaactg gcaggctggt ttgatggcag ctcggggatc
     6961 atgttcggta gaagtgcggc agaggatttg ttagattata aaatgaagga tgtatacgaa
     7021 gatctgtcaa aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg
     7081 ccacaactga cattgattaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg
     7141 actgttttgc aaacgtttat ttaaaaaatt ttttcctgcc ttggagtcta tcaaagacca
     7201 ataaatctaa atagaaaaga aagtgaaaga agctgacaaa
//
LOCUS       DADQRX010000119         7217 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.124, whole genome shotgun sequence.
ACCESSION   DADQRX010000119 DADQRX010000000
VERSION     DADQRX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.124"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="J8V14_002485"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="J8V14_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBA0922562.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="J8V14_002486"
     CDS             complement(919..1677)
                     /locus_tag="J8V14_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBA0922563.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="J8V14_002487"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="J8V14_002487"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBA0922564.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="J8V14_002488"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="J8V14_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBA0922565.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="J8V14_002489"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="J8V14_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBA0922566.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="J8V14_002490"
     CDS             complement(4433..5290)
                     /locus_tag="J8V14_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBA0922567.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="J8V14_002491"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="J8V14_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBA0922568.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DADQRX010000120         6644 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.125, whole genome shotgun sequence.
ACCESSION   DADQRX010000120 DADQRX010000000
VERSION     DADQRX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.125"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="J8V14_002492"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="J8V14_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="J8V14_002493"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="J8V14_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBA0922569.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="J8V14_002494"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="J8V14_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="J8V14_002495"
     CDS             complement(2143..2373)
                     /locus_tag="J8V14_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBA0922570.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="J8V14_002496"
     CDS             complement(2603..3421)
                     /locus_tag="J8V14_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA0922571.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="J8V14_002497"
     CDS             3582..4271
                     /locus_tag="J8V14_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBA0922572.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="J8V14_002498"
     CDS             4285..5787
                     /locus_tag="J8V14_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0922573.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="J8V14_002499"
     CDS             5800..6276
                     /locus_tag="J8V14_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBA0922574.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="J8V14_002500"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="J8V14_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DADQRX010000121         6531 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.126, whole genome shotgun sequence.
ACCESSION   DADQRX010000121 DADQRX010000000
VERSION     DADQRX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.126"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            121..330
                     /locus_tag="J8V14_002501"
     CDS             121..330
                     /locus_tag="J8V14_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBA0922575.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            323..472
                     /locus_tag="J8V14_002502"
     CDS             323..472
                     /locus_tag="J8V14_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBA0922576.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            507..1291
                     /locus_tag="J8V14_002503"
                     /pseudo
     CDS             507..1291
                     /locus_tag="J8V14_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1575..2660)
                     /gene="xerS"
                     /locus_tag="J8V14_002504"
     CDS             complement(1575..2660)
                     /gene="xerS"
                     /locus_tag="J8V14_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBA0922577.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2994..3227
                     /locus_tag="J8V14_002505"
     CDS             2994..3227
                     /locus_tag="J8V14_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBA0922578.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            3224..3631
                     /locus_tag="J8V14_002506"
     CDS             3224..3631
                     /locus_tag="J8V14_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBA0922579.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4768..5286)
                     /locus_tag="J8V14_002507"
     CDS             complement(4768..5286)
                     /locus_tag="J8V14_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBA0922580.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            6404..>6531
                     /locus_tag="J8V14_002508"
     CDS             6404..>6531
                     /locus_tag="J8V14_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922581.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFD"
BASE COUNT         2246 a         1011 c         1066 g         2208 t
ORIGIN      
        1 attaaggagc aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag
       61 gaacgctact atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa
      121 atggtggtta taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag
      181 agaatcatac gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat
      241 agaagattag gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag
      301 gtagaggagg attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca
      361 ttttactgct agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga
      421 ggggttaaaa cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa
      481 aagaagcaga aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc
      541 taccttatta tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca
      601 aaaaaacttt tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca
      661 aaagtggcaa aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg
      721 gatttttcag atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc
      781 aaaagttatt ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga
      841 atttacaaaa gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat
      901 ttgtatgtgg ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg
      961 gaaagatatt gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag
     1021 agccaaaacc tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact
     1081 agacgacaat acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg
     1141 catgtcgttt gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat
     1201 tatgagaaga catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca
     1261 tagtcatgct tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt
     1321 aaaacatgct gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt
     1381 gagagagata acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc
     1441 gacacctatt tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga
     1501 aacacactag aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta
     1561 tttatacatt atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg
     1621 gtatataaag ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat
     1681 gaatttgtct gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact
     1741 tgaattctaa agccttttga atattttgct accatccgtt cgattgcaat accggatata
     1801 cgcttggctt ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta
     1861 tatcgttctt tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata
     1921 ttgacagcat ctcttttatt tccttttcta gttactacca cattattctt atctaaagat
     1981 aggtcatcta aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt
     2041 gcattgatcg ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc
     2101 aaagtatttt tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga
     2161 aataattttt tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt
     2221 gctacttgat ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt
     2281 ttcgaaagat atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta
     2341 gatagaccta agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt
     2401 tcttttgtgc gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt
     2461 cttatcgagg tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag
     2521 agcttataat tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga
     2581 tcttgaacaa aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga
     2641 tatatttctg tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc
     2701 tataattgga ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt
     2761 aatttgtttc aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta
     2821 tacataaata gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta
     2881 attatataga aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt
     2941 aaaatcaaca agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa
     3001 ttaaagaaag aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt
     3061 ttcttaaaga aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc
     3121 gagcggcaat tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag
     3181 cattaactga atatgatact acttttagag agcttgtcga taaatgagta ttcattacct
     3241 agaagccaaa gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga
     3301 accccttggt gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc
     3361 agcttttggt gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca
     3421 attgataaag aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt
     3481 tgtctttttg aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt
     3541 agcggtatat attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa
     3601 cactataaag acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt
     3661 attagctgct atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac
     3721 tatatagaaa acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc
     3781 atatttttac tataccctat acttttactt ggtatattta tgaataatcg caaatttacc
     3841 cctatactac tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat
     3901 taaatacacc tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa
     3961 cagctgatag taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg
     4021 aacttgcttc tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact
     4081 gggattaaac taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg
     4141 atttatttat tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat
     4201 ttatttaaga tctcttcagt atcagtaata ggataggcga taggccacat accctttgat
     4261 ggttagaata aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc
     4321 tatctccaaa ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa
     4381 cccaaagtca gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat
     4441 tccatattta aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa
     4501 aaaatctttc ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc
     4561 attttatcga agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg
     4621 atgcaccctc tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag
     4681 ttagcaaaga acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc
     4741 cacaattgct tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt
     4801 tgttctaaaa gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca
     4861 tacagccgtt ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc
     4921 atcactcatt gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc
     4981 taattctttc aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa
     5041 ctgattccat ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt
     5101 ttcccatttt agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt
     5161 tctatccact tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa
     5221 aatatattta gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt
     5281 tctcattata ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat
     5341 ccacgatatt ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat
     5401 atgtgaatga tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa
     5461 ggagctaaaa aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat
     5521 tttcttcgta aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact
     5581 ttaagcgtta agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta
     5641 aacttcacga aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc
     5701 ttcgattgcg cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt
     5761 acggctcatc agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt
     5821 ctaaaaaagc gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta
     5881 cctgaagatc atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat
     5941 actacaatga tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac
     6001 ttgcaaaaga aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg
     6061 aaattttttt ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa
     6121 aaaataactt ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg
     6181 gtgtcgattt ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt
     6241 ctatagtatg gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata
     6301 tgttttagtg aactaccccc acttagcttg gataggctac aattaactag acagaaaaat
     6361 taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg
     6421 aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc
     6481 acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca a
//
LOCUS       DADQRX010000122         6312 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.127, whole genome shotgun sequence.
ACCESSION   DADQRX010000122 DADQRX010000000
VERSION     DADQRX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.127"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="J8V14_002509"
     CDS             290..1135
                     /locus_tag="J8V14_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0922582.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="J8V14_002510"
     CDS             1416..2636
                     /locus_tag="J8V14_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBA0922583.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="J8V14_002511"
     CDS             2633..5353
                     /locus_tag="J8V14_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0922584.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="J8V14_002512"
     CDS             5337..6281
                     /locus_tag="J8V14_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBA0922585.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2355 a          977 c         1218 g         1762 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aa
//
LOCUS       DADQRX010000123         6282 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.128, whole genome shotgun sequence.
ACCESSION   DADQRX010000123 DADQRX010000000
VERSION     DADQRX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.128"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..731)
                     /locus_tag="J8V14_002513"
     CDS             complement(18..731)
                     /locus_tag="J8V14_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922586.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            906..2330
                     /locus_tag="J8V14_002514"
     CDS             906..2330
                     /locus_tag="J8V14_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0922587.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2629..3336
                     /locus_tag="J8V14_002515"
     CDS             2629..3336
                     /locus_tag="J8V14_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBA0922588.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3323..3646
                     /locus_tag="J8V14_002516"
     CDS             3323..3646
                     /locus_tag="J8V14_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBA0922589.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3735..4772)
                     /locus_tag="J8V14_002517"
     CDS             complement(3735..4772)
                     /locus_tag="J8V14_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA0922590.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5093..6076)
                     /locus_tag="J8V14_002518"
     CDS             complement(5093..6076)
                     /locus_tag="J8V14_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBA0922591.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         1838 a         1246 c         1130 g         2068 t
ORIGIN      
        1 aaataatttt cccgagctta tttttcaaca aaaacagctt caaaaacaaa gtcttgatat
       61 aaatgtctcg ataccgaata ctcgaaaggt ttaccagacg atagagcaac aactttgtct
      121 atctcaatca taaattcacc tggatctatt tttaatattt ttgcgtcaaa ttcattcgca
      181 gtatctcctt tgatacgtat gtgcgaagtc tttatttcca gacccagttc atttgtgata
      241 taagcatata cagatttctt taaatgtttt ggttccaagc ctggtatgac tgctaatggc
      301 atgaatgttt gctcaatcga gtatggttga caatcaataa aacggcttcg tacaatatta
      361 tatacatagt cttttacact gatacttaat ttatcagcta cttttttcga tggaagttca
      421 attgagaaag tatggatttc tgaatctacg tgttcttcac caaccacgct aaacaaacct
      481 tcgtttggac caagaactcg tgagttatca attggtgttc tgacgacttc agtcccatac
      541 ccacttctac ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt ctttactgtt
      601 aacttgcttg tatgataaat aactgccaat tcatctcctg taggaagttt ctcaccaatt
      661 ttatactctc catttacaat ttttttcttt atatctaagt aaatttccat atacttcgca
      721 atttttttca atggtgaccc tccaatcaaa aacatatctt catctatact ttaacataaa
      781 taaaccgata aaatccacat caaaattata cacacaataa aaaagatata tatatttaaa
      841 caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa
      901 aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt
      961 ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc
     1021 gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg
     1081 atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct
     1141 ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc
     1201 ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag
     1261 tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg
     1321 aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg
     1381 gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt
     1441 atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat
     1501 tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta
     1561 gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg
     1621 atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg
     1681 ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg
     1741 aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac
     1801 taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc
     1861 catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat
     1921 accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc
     1981 attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac
     2041 gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc
     2101 atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt
     2161 ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt
     2221 cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat
     2281 taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa
     2341 ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac
     2401 tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc
     2461 ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt
     2521 ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag
     2581 tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag
     2641 ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt
     2701 ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg
     2761 atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc
     2821 catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg
     2881 atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt
     2941 ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga
     3001 caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg
     3061 ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt
     3121 ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat
     3181 agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat
     3241 gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc
     3301 ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg
     3361 ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga
     3421 tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt
     3481 atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa
     3541 tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat
     3601 cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga
     3661 gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat
     3721 cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt
     3781 cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag
     3841 accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa
     3901 gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag
     3961 tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg
     4021 atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta
     4081 atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta
     4141 cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa
     4201 ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa
     4261 atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt
     4321 tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac
     4381 ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg
     4441 tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg
     4501 cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca
     4561 tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg
     4621 acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt
     4681 atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa
     4741 ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt
     4801 ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt
     4861 catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg
     4921 ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt
     4981 attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt
     5041 gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt
     5101 cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag
     5161 tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat
     5221 agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat
     5281 cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg
     5341 gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta
     5401 cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg
     5461 tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc
     5521 aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta
     5581 tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga
     5641 tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg
     5701 ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg
     5761 ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt
     5821 ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg
     5881 taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt
     5941 ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata
     6001 caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc
     6061 aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct
     6121 ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa
     6181 aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc
     6241 cggaataaat ggtgttcaaa agcttgcatc tgcggtaata ag
//
LOCUS       DADQRX010000124         6238 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.129, whole genome shotgun sequence.
ACCESSION   DADQRX010000124 DADQRX010000000
VERSION     DADQRX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.129"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(315..602)
                     /locus_tag="J8V14_002519"
     CDS             complement(315..602)
                     /locus_tag="J8V14_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA0922592.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(622..846)
                     /locus_tag="J8V14_002520"
     CDS             complement(622..846)
                     /locus_tag="J8V14_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBA0922593.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1486..2019)
                     /locus_tag="J8V14_002521"
     CDS             complement(1486..2019)
                     /locus_tag="J8V14_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBA0922594.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2040..2984)
                     /locus_tag="J8V14_002522"
     CDS             complement(2040..2984)
                     /locus_tag="J8V14_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBA0922595.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3621..3707
                     /locus_tag="J8V14_002523"
     CDS             3621..3707
                     /locus_tag="J8V14_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBA0922596.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3828..4517)
                     /locus_tag="J8V14_002524"
     CDS             complement(3828..4517)
                     /locus_tag="J8V14_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922597.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4524..4802)
                     /locus_tag="J8V14_002525"
     CDS             complement(4524..4802)
                     /locus_tag="J8V14_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922598.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4762..5676)
                     /locus_tag="J8V14_002526"
     CDS             complement(4762..5676)
                     /locus_tag="J8V14_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBA0922599.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5658..6038)
                     /locus_tag="J8V14_002527"
     CDS             complement(5658..6038)
                     /locus_tag="J8V14_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBA0922600.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1649 a         1173 c         1055 g         2361 t
ORIGIN      
        1 cgtatttggt gcaaataacc gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc
       61 ccccccactc tccacaatac aaaaaagact agcaaatgct agccactttt tgtaaagttt
      121 cgcttgtttc accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt
      181 ttcgttgttc aaagattctt atgccattta ctattaataa atctataaat atgataattt
      241 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat
      301 tttattccaa agaattaact ccaaatacca atagaagccc atcctctata tacttgagat
      361 tccttactaa caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt
      421 aaaatatatc ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc
      481 tcactgattt ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata
      541 gttgcttcat tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc
      601 attataataa cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc
      661 attaacaata atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc
      721 tttgttacaa taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc
      781 gatgccagct agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt
      841 tttcatgatt gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta
      901 gtaattttat tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt
      961 tgaattcata atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat
     1021 aatgtgtata ctaccaagta acgcctgtac caagggatcc aggcgctgat tttgtgaatt
     1081 tttattggtc taggttaaaa gctcttttta aggcttggtt ttctgcggtt gtttctttct
     1141 aattctttgt gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg
     1201 ttatgctttt tacatatctc atattcattt attattcttt tctaaggtta ataataggat
     1261 aatctctttc agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta
     1321 caaatacttc ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt
     1381 tatttccttt gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga
     1441 tagaaccgaa tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg
     1501 ccaccatttt ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt
     1561 cgttgaacta ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt
     1621 ttcgatttgt tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat
     1681 atgagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1741 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1801 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1861 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1921 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1981 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     2041 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     2101 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2161 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2221 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2281 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2341 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2401 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2461 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2521 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2581 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2641 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2701 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2761 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2821 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2881 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2941 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     3001 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     3061 atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataaaca
     3121 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3181 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3241 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3301 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3361 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3421 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3481 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3541 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3601 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3661 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3721 gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta
     3781 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3841 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3901 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3961 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     4021 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     4081 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4141 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4201 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4261 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4321 cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata
     4381 acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt
     4441 tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag
     4501 ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc
     4561 atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc
     4621 tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt
     4681 gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca
     4741 ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc
     4801 aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt
     4861 tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     4921 ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt
     4981 ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa
     5041 ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc
     5101 acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg
     5161 aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg
     5221 tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc
     5281 atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt
     5341 ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt
     5401 gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt
     5461 ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac
     5521 acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat
     5581 gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct
     5641 acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg
     5701 atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg
     5761 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
     5821 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     5881 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
     5941 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     6001 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     6061 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
     6121 ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac ctccgtattt
     6181 ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctcaaaac
//
LOCUS       DADQRX010000125         5768 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.131, whole genome shotgun sequence.
ACCESSION   DADQRX010000125 DADQRX010000000
VERSION     DADQRX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.131"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            872..1840
                     /locus_tag="J8V14_002528"
     CDS             872..1840
                     /locus_tag="J8V14_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBA0922601.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1857..2381
                     /locus_tag="J8V14_002529"
     CDS             1857..2381
                     /locus_tag="J8V14_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBA0922602.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2757..3038
                     /locus_tag="J8V14_002530"
     CDS             2757..3038
                     /locus_tag="J8V14_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922603.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3040..3456
                     /locus_tag="J8V14_002531"
     CDS             3040..3456
                     /locus_tag="J8V14_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922604.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4086..5240
                     /locus_tag="J8V14_002532"
     CDS             4086..5240
                     /locus_tag="J8V14_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBA0922605.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5234..5656
                     /locus_tag="J8V14_002533"
     CDS             5234..5656
                     /locus_tag="J8V14_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922606.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT         2207 a          985 c          914 g         1662 t
ORIGIN      
        1 ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata tatgctaata
       61 atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag ctgaagcagt
      121 ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca cacgagcaaa
      181 tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag tatataaata
      241 tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag acaactttag
      301 ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat aagggattta
      361 tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa gagttcctta
      421 tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa tgcgcgaatc
      541 tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct cttttataat
      601 ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac tctcccaagg
      661 gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc
      721 tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga attagtacat
      781 cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa cgtgtatctc
      841 ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa agtatcatca
      901 tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt tgttcttttc
      961 tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat tcgagcaatt
     1021 aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac
     1081 aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg
     1141 tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata ttccttttgc
     1201 tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag aatgggtgcg
     1261 ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa tgtttcgatt
     1321 actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact ttcatgaatt
     1381 acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt tcaatccaat
     1441 acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat acggaaaagg
     1501 acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga
     1561 cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag aactagctaa
     1621 ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca aagaacaaaa
     1681 gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa atacccaaaa
     1741 taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag aactaaaaaa
     1801 agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta gattttatga
     1861 cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca gcaattaaca
     1921 aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac aagtttgtga
     1981 ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac gaaaactcaa
     2041 atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc aaccaagttc
     2101 ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat atgcaactac
     2161 aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa caacaactca
     2221 ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg acttatacgg
     2281 aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg atagaaaaac
     2341 aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa tttaaattaa
     2401 taaaaattgc tggttttatt cttctttttg cttttgcgag cagagcaaca tggacccatg
     2461 ttgcgatatt ttggtggcgc cccaatcccc gttttttcgt tgaatcttac tcggactgca
     2521 ctcgttttac agctgatttt tctgaatttt gaaagagcat aaggaaacgt ggtatcactt
     2581 tcttacgaaa ggtgctagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgctat
     2641 cacttttttg ccattgtggc taggctatgt atagtgtttt ggcgaaaaat agctatacaa
     2701 atactatatt tgtatggtat acataaatta tacgctgata cgaaaggaaa atttccatgg
     2761 acattttagt tcgaaatatc aatcccaaaa ttattgcaag aatagataaa cgttgtcagg
     2821 aactcacaac caaaacgggc aaaaaatgga gtcgaaataa atatctaaaa gttctaatcg
     2881 aaagtgattt tgaccgtcaa cttatgaatt acaaaaaaga tcaattcgat ttattactcg
     2941 aaaaatttat tgccattcaa acagaaaata cgaacatact gaatgactat gttcacacaa
     3001 acaaccaact cattgaaaca cttatcggag gaatataaca tgagaaaaac ttctgttcaa
     3061 aaacacgtaa ccctttatcc cagtcacgtt gacgttttag attccatcat cgaaaaaaat
     3121 ccagacgtca acaattattc agaagccatt cgttttttat gtatgagtta tgaacctgat
     3181 accaatacaa ccacaaccag taaccaagca aaactcaatg ccatgggaaa agaaatttct
     3241 atcatgacag aaatcatggc agaatttgct gatttatatc taaaagatac tggtgttctt
     3301 atcggtacaa attctaaagt gtatcaagaa gtgaaagaaa aaattgaaaa tcaactcaat
     3361 ccttcccgac gaaatcaacc caaaaatcgt caaaaagaaa ccaatcaaac ctttacttct
     3421 ggaaattttg acccaacaaa ctttctaaaa agataaaaaa agaaaccgat ccctaaaaaa
     3481 attcctcgat ttctttttag ggatcagtct tccagcaaga tttgcaagtt ggtaccaact
     3541 tgcgaaaaaa aagaaaaatc cgcttatgca acttcttctt tttgtatttt ggggcgctac
     3601 cccaatcccc aagtatcggt tatttaatag cgcggaaatc caaagaatag gacgtaccct
     3661 gaaacaaaaa aagactgctc tcacgagcag cccaaaatga gattatccta ttcatcaaca
     3721 taaaaatatt acaaagtgaa gaactagaga aagacaaaaa aagcatatca aatcctctca
     3781 aaaataacaa gtctttaaaa aatgacagat aaaaatgcac aaagaactaa aaaagagaaa
     3841 tcgcgtaaaa tcaagcatta gaagctagta actagctaga ccaattaaaa atcacaaagt
     3901 gagcgtctct atccattggt agaggggcta cttggcatag tgtacattat gttaactagc
     3961 tcgataaata acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt
     4021 atcgcatatt gtatatttta attatataat tataggtgta taccacttaa atttaacaca
     4081 aagacgtgat tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga
     4141 tgattggaat ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca
     4201 atatcaactc ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac
     4261 actccataac ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc
     4321 ttggttcgcc atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc
     4381 cagtattttc agacgctgat aatacgatta aatatattta taccgtcaaa aaaaatacaa
     4441 atggagaata ttcaggaagt atctcacaat ttttagcaaa agaacttcaa aatctagtag
     4501 atagtaatat aacagataca accttatttt ttcaagataa tagcatttat tattccgata
     4561 atggacaaat taaactactt tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt
     4621 cctttacaga aaaaaatgat cttatatccg taaatataaa aaataaaatt aaaaatttct
     4681 ctaaaaaaca aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc ggagataact
     4741 ttattatgat taattggcgt atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt
     4801 acgtagaagc tgctatctta tgtaacaaaa cagatgtacg tgctacttca gctagagcca
     4861 ttatgcatta tacctatcct aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc
     4921 aagatcaaat aataaaatat gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa
     4981 tactaaacct ttcagaggtc caaacacaac taagaaaagg aaatgccatt tatatgggaa
     5041 ctgctggtta tggaaattat agtaacagcc gtcatgcact tgcattattt gggtgggtca
     5101 atcaaggtgg ttatcaaact tactatgtgt ggaatccttg gtataactac ccaactgtaa
     5161 taagtgcaaa tgcaggacca gttacaattc ctgtacctgg aggtggtttt acttgggaca
     5221 ataccattac aaattggtaa ataaacccat tgctttagtt atgggtctag tgttagttct
     5281 tttcttaact agttgttcaa aaaaacaaga aatacaaaag actgatctga ttccttttct
     5341 aggaacttgg caaactaaag atcattcaga acgtctagta atcactaatt taaatacttc
     5401 aaaaaatata gaacaatata ccatcttagt aaaagagaac aatcataaaa ttcaaatcat
     5461 tgataaaaat ttaaatataa atgaacaagt tttcatttac aaagcaacaa ataatttact
     5521 aacactgcgc tctaataaaa aacaattaga ttacactttt agtttggatt ctccaaaaaa
     5581 gctcgtattt attttgacaa cgaatgatgg gacagaaggg acagctaaac caattgaatt
     5641 ttttaaagct gattgatgaa tagttgtttg atattacaaa acaataattt caccgaaggt
     5701 gaaacaagcg aaactttaca aaaagtggct agcatttgct agcctttttt gtattgtgga
     5761 gcgtgggt
//
LOCUS       DADQRX010000126         5585 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.132, whole genome shotgun sequence.
ACCESSION   DADQRX010000126 DADQRX010000000
VERSION     DADQRX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.132"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="J8V14_002534"
     CDS             complement(488..1297)
                     /locus_tag="J8V14_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA0922607.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="J8V14_002535"
     CDS             complement(1297..2043)
                     /locus_tag="J8V14_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0922608.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="J8V14_002536"
     CDS             complement(2061..2528)
                     /locus_tag="J8V14_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA0922609.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="J8V14_002537"
     CDS             complement(2528..2938)
                     /locus_tag="J8V14_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBA0922610.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="J8V14_002538"
     CDS             complement(2949..3962)
                     /locus_tag="J8V14_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA0922611.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="J8V14_002539"
     CDS             4179..4913
                     /locus_tag="J8V14_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922612.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="J8V14_002540"
     CDS             4897..>5585
                     /locus_tag="J8V14_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922613.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DADQRX010000127         5484 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.133, whole genome shotgun sequence.
ACCESSION   DADQRX010000127 DADQRX010000000
VERSION     DADQRX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.133"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..604)
                     /locus_tag="J8V14_002541"
     CDS             complement(146..604)
                     /locus_tag="J8V14_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBA0922614.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(617..1942)
                     /locus_tag="J8V14_002542"
     CDS             complement(617..1942)
                     /locus_tag="J8V14_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBA0922615.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1959..3371)
                     /locus_tag="J8V14_002543"
     CDS             complement(1959..3371)
                     /locus_tag="J8V14_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0922616.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3388..4152)
                     /locus_tag="J8V14_002544"
     CDS             complement(3388..4152)
                     /locus_tag="J8V14_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBA0922617.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4256..5131)
                     /locus_tag="J8V14_002545"
     CDS             complement(4256..5131)
                     /locus_tag="J8V14_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922618.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5386..5470)
                     /locus_tag="J8V14_002546"
     tRNA            complement(5386..5470)
                     /locus_tag="J8V14_002546"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5434..5436),aa:Leu,seq:caa)
BASE COUNT         1687 a         1151 c          802 g         1844 t
ORIGIN      
        1 ttttctaaat cataggagca acaaaaaaat tcgataatta acaaaaatca aaatgattaa
       61 atctagtgat tgaaacatat catttcaaaa aaattttgta aattcaccca taaatatcac
      121 cctatttaac ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt
      181 cttggttttg ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg
      241 tatctttttg tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt
      301 ttacctcctg gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat
      361 ctaaacccat atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc
      421 gggtaggaaa tatattatcg attattcctt taactggcgc atggatatat ccatcatgat
      481 tcgttatagc gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg
      541 ttaatggaat aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat
      601 gcacttttta tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt
      661 catctagtat tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt
      721 gtaaagcttt ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa
      781 cttgtgcctc catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct
      841 ccacaccagt tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg
      901 gaatcgcccc ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg
      961 aaatagctat atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt
     1021 ccacaatacc gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca
     1081 tgaccatatt agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata
     1141 agtaatactc taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca
     1201 tcatttgaaa ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt
     1261 cttttgttat tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat
     1321 aaatcgatgt ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac
     1381 gatcataacc gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat
     1441 taatcatttt tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat
     1501 ttgtataatt caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca
     1561 ataaaccttt gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc
     1621 ctactaatcc atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa
     1681 cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg
     1741 cacgaatgcg aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca
     1801 tatccatatt tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt
     1861 tttcattgtt caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta
     1921 tagtgataat ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg
     1981 aattctgcca taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt
     2041 ggtttaatga tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga
     2101 tccactacct ctacccgtag gcgtgtatta caactataga catccaggat attggctttt
     2161 cctccaaacg cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct
     2221 aactctgttt cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat
     2281 gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa agcaaaaaag
     2341 aaaattccga ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa
     2401 tcaacaatac atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc
     2461 cctgctaaac cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa
     2521 ctaaattcaa caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg
     2581 attcctttga ccatctcttt attttcttta taagctgctt tatagatagc tagacatata
     2641 gctggtatac caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga
     2701 tctccgttga taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag
     2761 cttcctaaat cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc
     2821 aaacgattca aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt
     2881 gcaaaattat caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg
     2941 attgtttgta gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa
     3001 actactggta atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa
     3061 ataattccac caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc
     3121 aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca
     3181 aaaccgattg caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca
     3241 gctgcaccaa ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc
     3301 gctaatggca tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat
     3361 ttaacattca tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa
     3421 aggatcattt ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt
     3481 acgaggatac acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca
     3541 catgacttcg atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc
     3601 cacactcgta ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg
     3661 ctcaaaataa tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa
     3721 atcatatttt tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg
     3781 atgatgactg tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc
     3841 attccattcc tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa
     3901 ggatctgaac tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca
     3961 cgtcaatgtt aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc
     4021 atgctcgaaa cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata
     4081 agcatccata atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat
     4141 cacttttccc atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca
     4201 tagtccattt actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat
     4261 agattcttgc tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa
     4321 attgctgttc gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa
     4381 atgtttcagt aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt
     4441 taaccaggaa tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa
     4501 cgtttgaata ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta
     4561 agctctttaa actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc
     4621 ctacttcatt tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta
     4681 ctgctatatc atattcattt ggataaagga atctcatttc tggcaacata attgctgtga
     4741 catctctatc ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat
     4801 agatatttga atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg
     4861 caaaatccat aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt
     4921 ctttagaatc taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt
     4981 tagcagaaaa gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca
     5041 atacagcatt atgattcagc tttttcatca catacaccgt taattcacct cctctatatg
     5101 ataaaacaaa acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa
     5161 agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca
     5221 ccctacataa tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt
     5281 actcgtttta tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa
     5341 aaaatatagc aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg
     5401 tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg
     5461 ccacgccggc aaatttaaaa ataa
//
LOCUS       DADQRX010000128         5481 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.134, whole genome shotgun sequence.
ACCESSION   DADQRX010000128 DADQRX010000000
VERSION     DADQRX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.134"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="J8V14_002547"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="J8V14_002547"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="J8V14_002548"
     CDS             complement(580..1353)
                     /locus_tag="J8V14_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBA0922619.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="J8V14_002549"
     CDS             1497..1847
                     /locus_tag="J8V14_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBA0922620.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="J8V14_002550"
     CDS             1828..2544
                     /locus_tag="J8V14_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0922621.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="J8V14_002551"
     CDS             2544..3992
                     /locus_tag="J8V14_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0922622.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="J8V14_002552"
     CDS             complement(4062..4571)
                     /locus_tag="J8V14_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922623.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="J8V14_002553"
     CDS             complement(4561..5286)
                     /locus_tag="J8V14_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922624.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DADQRX010000129         5347 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.135, whole genome shotgun sequence.
ACCESSION   DADQRX010000129 DADQRX010000000
VERSION     DADQRX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.135"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..1939)
                     /locus_tag="J8V14_002554"
     CDS             complement(8..1939)
                     /locus_tag="J8V14_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA0922625.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1957..2871)
                     /gene="pfkB"
                     /locus_tag="J8V14_002555"
     CDS             complement(1957..2871)
                     /gene="pfkB"
                     /locus_tag="J8V14_002555"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBA0922626.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2868..3620)
                     /locus_tag="J8V14_002556"
     CDS             complement(2868..3620)
                     /locus_tag="J8V14_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA0922627.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3779..3988)
                     /locus_tag="J8V14_002557"
     CDS             complement(3779..3988)
                     /locus_tag="J8V14_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922628.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(3999..5327)
                     /locus_tag="J8V14_002558"
     CDS             complement(3999..5327)
                     /locus_tag="J8V14_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922629.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1504 a         1154 c          932 g         1757 t
ORIGIN      
        1 tttatttcta tgcttctgct actgcaggag cttttctcaa taaaccataa atcacaccag
       61 atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc agtaagaata
      121 caacgaaaat cccgccgtga ggagccagta atttgattcc gaaagctccg actaatccgc
      181 cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca gctgctgcaa
      241 atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc aaaccagcat
      301 cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa attgccaatg
      361 gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca gttgcaacag
      421 tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga ccgcctaaat
      481 ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa ccactcatac
      541 ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg accagcaaca
      601 tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt ttaattccat
      661 ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca taaccagcta
      721 agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct aatgcaccag
      781 cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata aatcctgcta
      841 ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag ctagcagctt
      901 gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat tggtcgatca
      961 tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg tgtgatacgc
     1021 cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt ccttcttctg
     1081 aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg gctagattga
     1141 tcaactcttc tggtttacgg ataccatcac tgacaggacg attgatcagt tcttttccgt
     1201 caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct gcacgggcga
     1261 tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg actttgatct
     1321 ccacacccat ttctttcgct tttttcttca aagcatcttc tgccatgtaa gtgtgagcga
     1381 tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga gcagaagctt
     1441 ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct tcagctgttt
     1501 ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct acgaaatctg
     1561 catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg tttgcgcctt
     1621 ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct tcataatcta
     1681 ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg tttttcgcgt
     1741 gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg gctagaattc
     1801 cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt tggaccattt
     1861 catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct tctttgacca
     1921 ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg tgatttcaac
     1981 ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa aagctgttgc
     2041 gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac ggctgtaagc
     2101 cccaataaag ccagcaatca ttgaatcccc tgcacctact gaatttttca atgggcgaac
     2161 taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt ctccagccat
     2221 tgagatcagc acattctgtg ccccttcagc tagtaatttt ttcccatacg gaatgatttc
     2281 ttctacagat tggaaggttg tgttgtaaag ctcagctaat tcgtgattgt ttggtttgat
     2341 caacaacggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat caatcacgaa
     2401 atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt ttcgcaggct
     2461 agctggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc ctaatgcttg
     2521 cttcaactca tcgatttctt catctgaaat agatgggcct aagccattga tctcagtttc
     2581 agtgtctgat tttagtttga tattgattct tgtatcttct tttacggttg tgaacaaaga
     2641 ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc ctcctaaaaa
     2701 ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat tgatcccttt
     2761 tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt ttaacccatc
     2821 tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca ttgtattgcc
     2881 tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca acgctcggtt
     2941 atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt gaatttggta
     3001 tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt aacagctgcc
     3061 tcttctggat ccggagtcgt gaatccttgt tcaagatggg caccgttcat tcccataaat
     3121 gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa aacagcatta
     3181 gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc aactaaattt
     3241 gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag aaaaggaatc
     3301 atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt aatggtttca
     3361 gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt ttcactcata
     3421 ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct ttctagaaac
     3481 ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc attcaaaagg
     3541 tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag gattttttgt
     3601 ctgcgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc atattttttt
     3661 tctactagca atataaatca gtcaaaaacg ttcataaaaa ttataaatca gtcaaaaaaa
     3721 aataaaaaac cagtcagaaa gactggtttt ttattttttt gtctatcatc aagccgtttt
     3781 atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt tttctttcct
     3841 tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa ccgatgactg
     3901 atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat gccggaacca
     3961 ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg taaagtaatc
     4021 aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc acctttcttg
     4081 acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac atcagagagt
     4141 ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac atagattctt
     4201 aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag atattcccta
     4261 atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc gataacttta
     4321 tagcacatca tgcgtgatcg attctcgtca acccaaggaa aatacgcctg agcatttagt
     4381 gttccgcgaa cacgcctttc tttataatct ccttgttgga tatcataact ttttgttaca
     4441 aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata ctggacagat
     4501 tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa accgacacca
     4561 ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg aaacatttga
     4621 ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat gacttcgttt
     4681 tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt agggatgatt
     4741 tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa accttggtaa
     4801 aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt ttgttgtttc
     4861 attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg agagatctgt
     4921 tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac cccatgagca
     4981 atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa aacagagata
     5041 gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca ttcgttctcg
     5101 tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat tttttcttga
     5161 agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa tgttggctgg
     5221 atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc ttcttcattt
     5281 aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt ctcacctctt
     5341 tcaaatc
//
LOCUS       DADQRX010000130         5312 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.136, whole genome shotgun sequence.
ACCESSION   DADQRX010000130 DADQRX010000000
VERSION     DADQRX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.136"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="J8V14_002559"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="J8V14_002559"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA0922630.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="J8V14_002560"
     CDS             complement(1298..2134)
                     /locus_tag="J8V14_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922631.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="J8V14_002561"
     CDS             complement(2318..3535)
                     /locus_tag="J8V14_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA0922632.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3918..4001
                     /locus_tag="J8V14_002562"
     tRNA            3918..4001
                     /locus_tag="J8V14_002562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5238)
                     /locus_tag="J8V14_002563"
                     /pseudo
     CDS             complement(4241..5238)
                     /locus_tag="J8V14_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DADQRX010000131         5189 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.137, whole genome shotgun sequence.
ACCESSION   DADQRX010000131 DADQRX010000000
VERSION     DADQRX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.137"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="J8V14_002564"
     CDS             137..748
                     /locus_tag="J8V14_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBA0922633.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="J8V14_002565"
     CDS             821..1750
                     /locus_tag="J8V14_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBA0922634.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="J8V14_002566"
     CDS             1908..2645
                     /locus_tag="J8V14_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBA0922635.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..3871)
                     /locus_tag="J8V14_002567"
     CDS             complement(2693..3871)
                     /locus_tag="J8V14_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBA0922636.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..3977
                     /locus_tag="J8V14_002568"
                     /pseudo
     CDS             3894..3977
                     /locus_tag="J8V14_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4098..4499
                     /locus_tag="J8V14_002569"
     CDS             4098..4499
                     /locus_tag="J8V14_002569"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922637.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="J8V14_002570"
     CDS             complement(4706..>5189)
                     /locus_tag="J8V14_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922638.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DADQRX010000132         5176 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.138, whole genome shotgun sequence.
ACCESSION   DADQRX010000132 DADQRX010000000
VERSION     DADQRX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.138"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="J8V14_002571"
     CDS             complement(136..1098)
                     /locus_tag="J8V14_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0922639.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="J8V14_002572"
     CDS             complement(1100..2539)
                     /locus_tag="J8V14_002572"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBA0922640.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="J8V14_002573"
     CDS             2724..4670
                     /locus_tag="J8V14_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBA0922641.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="J8V14_002574"
     CDS             4934..>5176
                     /locus_tag="J8V14_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0922642.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DADQRX010000133         5173 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.139, whole genome shotgun sequence.
ACCESSION   DADQRX010000133 DADQRX010000000
VERSION     DADQRX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.139"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..968
                     /locus_tag="J8V14_002575"
     CDS             <1..968
                     /locus_tag="J8V14_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBA0922643.1"
                     /translation="DYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFW
                     NYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELF
                     IIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLL
                     EYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKS
                     FGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSV
                     FFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            965..1684
                     /locus_tag="J8V14_002576"
     CDS             965..1684
                     /locus_tag="J8V14_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBA0922644.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1699..2784
                     /locus_tag="J8V14_002577"
     CDS             1699..2784
                     /locus_tag="J8V14_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA0922645.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2920..4452
                     /locus_tag="J8V14_002578"
     CDS             2920..4452
                     /locus_tag="J8V14_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBA0922646.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4630..4851
                     /locus_tag="J8V14_002579"
     CDS             4630..4851
                     /locus_tag="J8V14_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBA0922647.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1793 a          552 c          997 g         1831 t
ORIGIN      
        1 aagattacta taaagtattc gctataattg aaaaattatt ctggatttca ctgccacttt
       61 gtttaattga aagattcatg gtcacctatc caactggaac aattgtagga gatatgatag
      121 gtggagtttt ttggaattat tcaggttgta atatgccatt gaatttaatt ttgtgtgtga
      181 cagttactgt tgttacaatc ggatatttca agggagaaaa aaatttcatc cattttattt
      241 tatgtgtttg tgcagcactt tttatgtctg cattagctga attgaaagtt tttattcctg
      301 aattatttat aatatttgtt gcaatagtat tcactttaaa agttgatttt aaagtagtga
      361 tcaagatatt atcatgcgta atacttgtgt cgatagtatt atcctttttt ataacaatat
      421 ttattacatt gaacgataat ggacaaagct atgctgataa tttcacaatt caaggtcttt
      481 tagaatatgc gtcaagagat tctggatatg atggtgttgg tgatttaaat cgaataactg
      541 gtatctctag agtagtagat gaaatattta ataataattt tagattagtc ctaatgggag
      601 taggcattgg caatgcagag tttaccaatt tttttgtcag tgagttttat aaatcgtttg
      661 gtaatcttca ttatcagtat tttcatcata tttggttatt tatagaagtt ggaatattag
      721 gagtttcgat attcctattt tttataataa agacattttt caatgcaaaa agatctttta
      781 tgaaggataa agatgcaagt cttgttaaag ttattgcaat tatgtcggta ttcttttttg
      841 tgtataacac aacacttagg ggtgaaacaa cggggccact gatttatatg attttagcca
      901 tgcctgaagt tgtgaaaagg aaagagcatt attatagtga aaaaaataca aaaggagaga
      961 tattatgatt cctaagaaga tacattactg ttggttcggg ggaaatccac ttccgaaaaa
     1021 agatagaaag tgcatagaaa gctggaaaaa gtattgccct gattatgaga ttattgaatg
     1081 gaatgaatca aattatgatt taaataagaa ccgttatatg gccgatgcat ataaagaacg
     1141 taaatggggt tttgttccag attatgcaag atttgatatt atttataatc atggtggatt
     1201 ttatttagat acagatgttg agctaattaa gtcgttagat atacttcgtg aaaatcatgc
     1261 ctatatggga tttgaagggg aaatctgggt taatggcggt attggttttg gtgcagaaga
     1321 aggacattct gttattaaag gtttaaggga tatttacgat tcattaagtt tttatcaaaa
     1381 tgatggtaca ttgaacttga aaccgagtcc gtattatatt acagattatc ttgaatctat
     1441 tggattggtt cgcgataaca gcgaacaaat aataaatggc ctgcatatct atcctacgga
     1501 ttattttgct gccaaggatt atgatactgg aaaaatttat aaaactagta atacaataag
     1561 cattcatcaa tataatgcat cttggaaaac acctaaacaa aaatttgttt atagattaaa
     1621 atcaacaatt ggacaaaaaa aatttaacag gttacttgca ctgaaaaaat ttttacactt
     1681 ataaaggaga aaagtacttt gaagaaaatt tgtttcatta gtggtgtaat ttctcgttct
     1741 gggggaactg aaagagtagg gagtattatt gccaatagct tgtctgattt aggttatgat
     1801 gtcactattt taagcttttg gaatagtgga gagccttatt ttgggttaga taagacgatt
     1861 aaggttgatt atatgttaaa tcctaaaacg gagggaaaat tatatagaac ttatttatat
     1921 ccggtttgga aattgcacaa gtatatatgc aaaaatgaat ttgatatagt aattgatatt
     1981 gatactttgt tgtctttgta tactacaagg gcaattaaag gaacaaagtg caaattgatt
     2041 tcttgggaac attttaatta ctggacaatg gaaatgctga aagaaaagaa gagattcaag
     2101 gctaaaaaat tgataaaaaa atatgcaaag aggcttgtgg ttctcacaga agaagatcgt
     2161 caaaaacata ttgatgaata tagtttatca gaagattttg ttataactat gccaaatccg
     2221 tgtatatcta atatcgagta cgaatatcat tatgataaaa agagatttct tgcagtaggg
     2281 cgattagtgg aagagaaaaa ttttgctgac atgataagtg cttggaaaat tgtagaaaaa
     2341 aaagtagatg attgggatct tatcattgta ggaaaaggtg aattagaaaa agatcttcgc
     2401 cagcagataa aagatttaaa tctgcaaaga gtaactatag cagggcattc agaccatgtg
     2461 gaggaatatt ataaatctgc atcttgttat gtgcttagtt ctgtttatga aggatttcct
     2521 atggttatat tagaagcaca gtcttatgga ttaccagtta tttcttatga ttgtaaaact
     2581 ggtccgagag atttggtaca tcataatttt aatgggatgt tggttgaaga taagaatata
     2641 gaccaattag ctaagagcat gataatgttt actaagaata ctgatttagc aatgagaatg
     2701 tcactgaacg catataataa tgtacaaaaa tttaatttga aagaaattac aaaacaatgg
     2761 gttgctttga ttgaaaatgt atagtttttt gtggttgtcc aaacatgaaa attagcgtaa
     2821 ttaatcaaag agctagaaga aacctattac atttcaagat tttagaaatt atatagtatt
     2881 aggtaacaat aaaaaataga aagtttggag ccatatgata tgataattaa tcgtaaacaa
     2941 aatgctaaaa ggaatataat tactggaaca atttttaagt tatatcagat tatcattccc
     3001 ttttttatgc gtacacttat gatttatcag atgggaatgg agtatgctgg attgaacaac
     3061 ctgttcacat ctatatttca aatgttaaat ttagcggaat tgggaatagg tgctgcacta
     3121 actgtaagta tgtataaacc tatagcagaa gacaatagaa aagagatttg tgctttactg
     3181 aacgcatata aaaagtactt ttcaattatt ggatggatag tgtttacatt aggattgtta
     3241 tgcattcctt ttttgagatt tttagttaaa ggttcgattc ctggaaattt aaatatgaca
     3301 gttttgtatt taatgtattt gataaatact gttttgtcgt attggttatt ttcatataga
     3361 aagagtctaa tatatgcaca tcaaagaaat gatgtaatta acgtgtctat gctaattact
     3421 tttacattcc aatttgtaat gcaggcatat gttctgattt tcttaaaaaa ttactatttg
     3481 tatttattgg cttctatttt gggacaaata gttcttaact taatgtgtgc tgttgtttct
     3541 caaaagatgt atcctgatta tactcctgtt ggtgagttag agaaagaaac tattgacgat
     3601 attataaaaa aagtaaaagg attagttaca aacaaggtag gcggtacaat attacgatct
     3661 tcagattcaa ttattatttc atctttctta ggtctttcag tacttgcgat ttatcagaat
     3721 tattacttta ttatttctgc cataatgagt atatttgcaa taatttatga atcatgtata
     3781 gcaggtatag gtaacagtct tattgtagaa agtcaagaaa aaaattatat agatttttgt
     3841 gatattacaa caattatagg ttggtttctg gcaattagtt gtgcatgctt tattacatta
     3901 tttcagccgt tcatgattat ctgggtggga agtgataatc ttatgagtat ttcatttgtt
     3961 atatttatga tagttcattt tttcttatat gaaattgatc agttaattgg gttatacaaa
     4021 gatgctgctg gaatatggta cgcagacagg tttagaccat taattacagc aatgataaac
     4081 attgtattaa atatcatcct cgttaattgg ttcggattat atggagtttt ggcggcaacc
     4141 attgtttcag tattgttggt tgatttaccc tggttattat ataatgtttt ccaaaatata
     4201 tttaaatttg aatcggcagg taaatatgtt tggaagctca taagtttgtg tgtaatagct
     4261 tttgttgctt cttatttaag ttattttgct tgttcgtatg ttaatatcgg aaatttcaaa
     4321 gtgataagtt tagtgataaa tttagttata tcggttgtca tatcgatttt agttattctt
     4381 ctgtttaata tccgtaataa gaagtttaag tatattttga aagttcttac gtcggtattg
     4441 gtaaaaagat gagaagagac atcagaatga tatataaagt aactacaatt tttaggctgc
     4501 cgaataggcg aacgatgtat agtttttgtt ccaacgaaga ctcttgttga aattggagat
     4561 gatatgaagg agttacaatt ttgactcatg tttatgattg atcggtttta aaagtagtat
     4621 ggagaagctt tgggatcatc ggagaggatc aaaataggta ataatgtttt tataggaatg
     4681 aagtctagaa tactaaaggg tgtacatata ggaaataatg tagttatagg tgcaaatagt
     4741 ttagtaaaca aggacgtatc agacgattgt gttgttgcag gaaatctagc aaaagtaatt
     4801 atgtccctag atgaatatta taaaaagata aacaatatga agaatccgta aaacttgtga
     4861 aactataccg agaataatat gggaaaaata ttgatgaaaa agcattatat gagtttttag
     4921 ggctgtttac agatggagat gacaatttgt cgaagagttg ggagcaatgc aaaatttagt
     4981 aggggattat aaacagactg acaaggtatt taaagaacat gcacaagtat ttcataatat
     5041 tgaagaattt ttgaatactg tgagatagga gttgctacat tgagaaaagt atgtattttt
     5101 ccaatgtaaa agattacgag tttgaaaacg acaccataat attatttttt aagaagatat
     5161 tatagagctt agg
//
LOCUS       DADQRX010000134         5152 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.140, whole genome shotgun sequence.
ACCESSION   DADQRX010000134 DADQRX010000000
VERSION     DADQRX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.140"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..561)
                     /locus_tag="J8V14_002580"
     CDS             complement(52..561)
                     /locus_tag="J8V14_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBA0922648.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(673..1671)
                     /locus_tag="J8V14_002581"
     CDS             complement(673..1671)
                     /locus_tag="J8V14_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA0922649.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1872..2456
                     /locus_tag="J8V14_002582"
     CDS             1872..2456
                     /locus_tag="J8V14_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0922650.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(2533..3708)
                     /locus_tag="J8V14_002583"
     CDS             complement(2533..3708)
                     /locus_tag="J8V14_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA0922651.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            3790..4170
                     /locus_tag="J8V14_002584"
     CDS             3790..4170
                     /locus_tag="J8V14_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBA0922652.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            4244..4606
                     /locus_tag="J8V14_002585"
     CDS             4244..4606
                     /locus_tag="J8V14_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922653.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1684 a          891 c          958 g         1619 t
ORIGIN      
        1 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
       61 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
      121 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
      181 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
      241 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
      301 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
      361 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
      421 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
      481 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
      541 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
      601 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
      661 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
      721 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
      781 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
      841 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
      901 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
      961 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
     1021 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
     1081 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
     1141 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
     1201 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
     1261 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
     1321 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
     1381 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
     1441 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
     1501 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
     1561 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
     1621 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
     1681 tcccttttca cactatagca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
     1741 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
     1801 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaatattag
     1861 gaggaaacaa tatggcattc ccacaactag atttaaaaag tgaaaaagga ccaaaagctg
     1921 ttatcaaaac aaaccgtggt gacattactg ttcaattatt ccctgaacta gcaccgaaga
     1981 cggttcaaaa tttcattgaa ctttccaaaa aaggttatta tgatggcgtg atttttcacc
     2041 gcgttattcc tgatttcatg atccaaggcg gcgatccaac tggaacagga atgggtggcg
     2101 aaagtattta tggcgaaagt tttgaagatg aattcagtcg tgaactgttc aaccttcgcg
     2161 gagctttatc tatggcaaat tcaggtccaa acacaaacgg aagccaattc tttattgtaa
     2221 ataacacaaa tgtgccagct aatatgcttg gacaactaga aggtgctgga ttcccatcag
     2281 aaatcattga agcgtataaa ggtggcggaa ctccttggct agatttccga catacagtct
     2341 ttggacatgt actagaaggg atggacacag tagatgaaat cgcaaatgta caacgtggtc
     2401 cacaagaccg acctgtacat gatgtagtga ttgaaacaat tgaaatttca gaatagtttg
     2461 gtatggtacg gagtaattat gaaaaaggac gaaacctagt aaggtctcgt ccttttattt
     2521 tgaataaatg atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag
     2581 taaagtttct tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa
     2641 cgtgatgcca ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg
     2701 gtcgtctgaa atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat
     2761 tattctcact ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc
     2821 tagataagga atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc
     2881 aatcagtcca aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc
     2941 agctttcatc tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa
     3001 agtcttggtt gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag
     3061 catggaagta aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag
     3121 aatcacttga tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac
     3181 acgccctcca ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat
     3241 ttttttctcc atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct
     3301 tcgaacgatg tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc
     3361 atgaatcaaa atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt
     3421 taacacacca cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg
     3481 ttcccaatga atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc
     3541 atgtttgatg tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc
     3601 tgctacccac aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt
     3661 tacactttct gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc
     3721 agtcattttc gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga
     3781 gggaagtaga tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag
     3841 ccttatggcg cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa
     3901 gtacaatccg gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca
     3961 gttcagatca tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta
     4021 gaaaaaacaa atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt
     4081 aagatcggat ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct
     4141 gaactagaac ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga
     4201 atttttatgt aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt
     4261 tgctggtaca attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat
     4321 tgatgacaag ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa
     4381 tatacttaat ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga
     4441 attgacaaat acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca
     4501 aaagaatcaa agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg
     4561 ctctgttctt ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc
     4621 gaacaaagaa gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac
     4681 aatgaaatgt tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa
     4741 acggttgtat aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa
     4801 tttatagttg accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga
     4861 tgttatatca catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg
     4921 ataagataac tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa
     4981 cttcaaaaaa gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag
     5041 atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc
     5101 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cgggaaacac tc
//
LOCUS       DADQRX010000135         5109 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.141, whole genome shotgun sequence.
ACCESSION   DADQRX010000135 DADQRX010000000
VERSION     DADQRX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.141"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="J8V14_002586"
     CDS             join(107..335,335..1248)
                     /locus_tag="J8V14_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA0922654.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="J8V14_002587"
     CDS             1483..1935
                     /locus_tag="J8V14_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922655.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="J8V14_002588"
     CDS             1949..2638
                     /locus_tag="J8V14_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922656.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..>5109
                     /locus_tag="J8V14_002589"
     CDS             2763..>5109
                     /locus_tag="J8V14_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBA0922657.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKL"
BASE COUNT         1738 a          780 c         1045 g         1546 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaactta
//
LOCUS       DADQRX010000136         5087 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.142, whole genome shotgun sequence.
ACCESSION   DADQRX010000136 DADQRX010000000
VERSION     DADQRX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.142"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="J8V14_002590"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="J8V14_002590"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBA0922658.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="J8V14_002591"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="J8V14_002591"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBA0922659.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="J8V14_002592"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="J8V14_002592"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBA0922660.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="J8V14_002593"
     CDS             complement(4028..5044)
                     /locus_tag="J8V14_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA0922661.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DADQRX010000137         5048 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.143, whole genome shotgun sequence.
ACCESSION   DADQRX010000137 DADQRX010000000
VERSION     DADQRX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.143"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="J8V14_002594"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="J8V14_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBA0922662.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3249)
                     /locus_tag="J8V14_002595"
     CDS             complement(2944..3249)
                     /locus_tag="J8V14_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922663.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3394..4599)
                     /locus_tag="J8V14_002596"
     CDS             complement(3394..4599)
                     /locus_tag="J8V14_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBA0922664.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1601 a          919 c          678 g         1850 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattact ctttcttaga ttaaacttca gagtattcaa
     2641 atagcggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc attcctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat taaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg cttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaattt tttgttaaaa tccaatttcc tcaactcctc caaactacgt
     3361 aaatgatata gtctcataat aaaataaaac ctctcagcta atgttgttga aattatccac
     3421 taaacctaat tgatattgct taggagatac acctgcgtac tttttgaata ctctcgaaaa
     3481 gtgtgaagaa tctgaaaatc ctaaacctaa agaaacttca ctgatagtat ggtttgattt
     3541 caacattatt ttagccgtag aaatttttcg cttagagata taattataaa tcgtaatatg
     3601 catttcattc ttaaagcttt ctcttaattt agtttcactt acagaaaaat aattagctat
     3661 ttcctttaca gtaattcttt tatagatatt taagccgatg aattgaacga cagaagaaat
     3721 taagggagaa agatgctcta tcttagtttt tccgatttcc ttagtataat aaacaatccc
     3781 agaatccctc acttttaaga cctcaggtaa cgatacagct aattcgtttt cttgtatcaa
     3841 agcatctcta ctttgatacg cttctattat atccattcct aatgttatag acgtttgtgc
     3901 taacttttca aaaacaatga tagaataatt tttttcagac cgaatcgtat ctccactaac
     3961 acttggaatt attccattac tcagattaaa aaccatgttt tctaaatttc gaatatctcc
     4021 atttttaaca tattccagta ttttattttc ataaagaaac agatatattt ctggatcgta
     4081 attttgtgat aacagattat ctattttgat ttgctcgata gttttactta gatttcctgt
     4141 atattcaatt aacggttcat ggaatagatc ttctattttt ccagtaaaaa aataatgaac
     4201 taaaatcaga atatctcgaa tgtctcctaa agaatacaaa gtcaaatttt gcaatgaatt
     4261 atagagtcgt tctctatcag ccattgcaac attcaaattg ttcattttta gtttaaaaaa
     4321 atctttatca atattatttg ctctaaatgg accaaataag taatatctgg ttttaaacat
     4381 gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt tagttttttt
     4441 aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc tatctgagcg
     4501 atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact ttaaacaaac
     4561 atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc tctttccttt
     4621 ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg tcaaaataag
     4681 ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc accgtaaaaa
     4741 gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat ttacatatat
     4801 ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata cagataatag
     4861 gaatttttcg tttctaaata ctccaatttg ggaatccaat aaaaattttc gttcaaatac
     4921 gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat aaaaaataca
     4981 atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt tattatacgt
     5041 aaaaagct
//
LOCUS       DADQRX010000138         4994 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.144, whole genome shotgun sequence.
ACCESSION   DADQRX010000138 DADQRX010000000
VERSION     DADQRX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.144"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..663
                     /locus_tag="J8V14_002597"
     CDS             <1..663
                     /locus_tag="J8V14_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922665.1"
                     /translation="MELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFK
                     QSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFI
                     RKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSL
                     EELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKH
                     IS"
     gene            884..2647
                     /locus_tag="J8V14_002598"
     CDS             884..2647
                     /locus_tag="J8V14_002598"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBA0922666.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2731..3189
                     /locus_tag="J8V14_002599"
     CDS             2731..3189
                     /locus_tag="J8V14_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA0922667.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3298..3981
                     /locus_tag="J8V14_002600"
     CDS             3298..3981
                     /locus_tag="J8V14_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBA0922668.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4128..4742
                     /locus_tag="J8V14_002601"
     CDS             4128..4742
                     /locus_tag="J8V14_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBA0922669.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4758..4964
                     /locus_tag="J8V14_002602"
     CDS             4758..4964
                     /locus_tag="J8V14_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922670.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1770 a          795 c         1025 g         1404 t
ORIGIN      
        1 atggagctgt taagaaaaga tttcgaagtt gtttataaaa attttgttct caatgaccta
       61 tcactgaatg aattgatttt acagattatt agtatcgctg aaatcgacga tgcagaaaat
      121 tttattttca agcaatcctt tttacaattt gtccgtacgt tgtgggcaga tggacaagta
      181 tttttaccgg aaaggatcta tcttctatca gaaaaagaac aacttctgta cggaaaaatt
      241 gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat
      301 ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc ggggagacag
      361 gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac aattttttta tcgaacccaa
      421 tttcatttgt atgcggaaga aggcatcgct gaaataaatc ctattttatt tcgttcatta
      481 gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct
      541 agctatcaaa atggattaga aactgaaaag acaaagatct atccaatctc attaaaaggt
      601 gcaaacatcc atttgtatca tattgctcag gaacttcacc tattaaaaaa acatatttct
      661 tgatgagacc ttaattagga gaccttctac aaagcttttt aattgtctgt agaaggtttt
      721 tatttgagtc ctgattcgtg cgaagatcta cttatataac aagctttctc tgaaattttt
      781 ttaaaatgct attgacttag agtcaactct aagtagtaga atagataaag tgtaacaaat
      841 ctattttcat taaaagaaga tagtacggag gagagaatgt attatgagta cagtattaca
      901 tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa
      961 acagttggca gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg taggtgggca
     1021 cgttggtccg aatctaggtg tagtagaatt aacgattgcg tttcattatg tatttgattc
     1081 accaaaggat aaaatcgttt gggatgtttc ccatcagtct tatggtcata agcttttaac
     1141 tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat attccaacca
     1201 acatgaaagt aaacatgact attttacagt ggggcacaca tctacatcta tcagcttagc
     1261 aacaggttta gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg tagcatttat
     1321 cggtgacggc tccctaagtg gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt
     1381 acaatccaat ctaatcgtat tagtaaacga taatgagatg tctattgatg agaatcatgg
     1441 cagtttatat aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct
     1501 atttaaagca atgggatttg attatcgtta cgtggataat ggaaacgatc tggaaacggt
     1561 gatccgttta tttgaagaag taaaagatat tgatcatccg attgtacttc acgtccatac
     1621 ggaaaaaggt cgcggttatc agccggcaat cgataataaa atgaaatatc attggcatgt
     1681 accatttgat ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa catttaatat
     1741 gatcatcatg gattatttgg ataagaaaat gcaaacaagt acagctccta ttgccgcagt
     1801 caatgcagcg ataccaggcg cctttggact gaaaacattc caagaaaaac atccagatca
     1861 ttattttgac gtggggatcg ctgaacagca ctctatcact ttcgcttctg ctttggcttc
     1921 tcagggagta cgtccagtcg tcttccaatc aagtactttc ttgcaacggg catatgatca
     1981 attatcacat gatttagcga tcaacgagaa tccagtgatt attttagtcg gtggcggaac
     2041 gatctcagct agtgaccgaa cacaccaagg aacatttgac attccttact tatcaagtat
     2101 cccgaacatc aaatatcttg ctccagcaac aaaagaggat atgatctcta tgctagattg
     2161 ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt cctacacatg gtgtagaaca
     2221 tggtacagct atcgcaaatg attatagtat tccagcctat caaacagtaa ataatggagg
     2281 aaaagtagca attttagctt taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc
     2341 ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca cagaaaatga
     2401 cgtagaaaca ttagataaat taaaagaaac acacgaatta gtcatcacat tagaagatgg
     2461 tagtttgtca ggaggatttg gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa
     2521 cgtattaaat tttggtgcag agaaagaatt cacggatagc gttcctgtag ggcagcttta
     2581 tgaacgttat catttaacac caaaacaaat cgttgaagat agtctgcgtg tcttgaatat
     2641 tcaataattc attatgctca ccatccagat aattgtttat actagtatct ggatggtttt
     2701 ttcattataa aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga atatgtcaaa
     2761 aagttccaag aaaaatatcc tagttatcaa tcagaaaaat taggtttctt ttcatttggg
     2821 gatacagaaa aaatggcaga cgagttgagc gaactagttg taaaaaagat aaagactggt
     2881 acatcaagtg gttacgatct gtataaacaa gagaatgagc ctttaccaaa agttggtcaa
     2941 ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa tataaatgtg
     3001 accatcttgc cttttagtga agtcacgaaa gagcatgctt ggaaagaagg agaaggggat
     3061 cggacattag cttattggcg gaaagtccat gctgagtttt ttaaacatgc ttataaagaa
     3121 gcgttgaaca tagaatttac agaagagaag ctggtcgtat acgaagaatt tgaagtaatt
     3181 ttcttttagt aagaaaggtc tgctgaaaga aatagctcat tattgtctcc tgaatagaaa
     3241 agggttacat ttttatatga agctgtattt ttatataaca ttactggagg agatattgtg
     3301 gatacactgg aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt ggaaaacgag
     3361 ccgctacaca gttcttttat tatggggaga atggctttgg ctgcaaaaga aggcggagca
     3421 gttggtattc gagcaaatac gacagaagat atcaatgaaa tcaagcgaat cgttgattta
     3481 ccggtaatag ggattatcaa aagagattac gatgggtcag aagtgtttat tactgcgaca
     3541 gaaaaagaag tggaagaact tttggagacg caagcagaaa tcattgcact ggatgcaact
     3601 tctcgtaaac gtccgacaga aaaaacaact gctgaacttg tccaaatgat tcacgaacac
     3661 ggtcggttag ctatggcaga tatttcgact tttgaggaag cgcttcaagc gcaagaagat
     3721 ggttttgatc ttttgtctac gactttagca ggctatacag aatattcacg aaagacgcag
     3781 acacctgatt ttgatttatt gaatcaaatc atagaacaag tcgatatccc agtaattatg
     3841 gaaggacata ctgactctcc tgaacaagta gagaaagcat atgaattagg tgctttttca
     3901 gtcgtagttg gatctattat cacccgtccg caattgatca ctaaaagata tgtcgaagcc
     3961 gcaagtaaag caaataacta aaaaacacgc acggcacaga ttcttgacaa aaaagaaaaa
     4021 ttgatagata gtttaaagca gattctaaga agatgaaaaa agcattactt ttcattttgt
     4081 cttcaagatc tgctttttaa cgaattttat agaaagaagg cgaaaccatg tttgaacgga
     4141 gtccttcaga attgacagaa cgtgaaaatt acaagttgct gattggttca gtcatcccta
     4201 gaccagtagc agtagtaacg actaaatcag caaaagatat agtgaatatc gcacccttca
     4261 gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc cagagaaaac
     4321 aagatgcagt aaaagacaca gcaaagaata ttcttgagac aagagaagct gttatccata
     4381 tccttgatga ggacaacgta gaagaagtaa ataaaacagc agccaatctg cctaatgagg
     4441 aaagcgaact gtacctgact gacttgactt taactgattc agtaatcatt tcagtaccag
     4501 cattgacaga agcaaaagtt cgttttgaaa catccttata tgaacatgtc gagatacgga
     4561 ataaaaataa agtaacagct gatttatttt tgctagaagt acagaaatat catttaaatg
     4621 aaacagtcta tgatgataag acaggaagga tcgatccaag aaaattgaat gctgtaagcc
     4681 gtttggcagg aaatgattat gcagcaatag gagaaatttt cacgattgca cgtcccgaat
     4741 aaaaaggagc actagttatg ttaaaaataa gctataaacc atcgacagat tcaaaagaaa
     4801 tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg gcagtactta gaagttccgc
     4861 cattgcaaga ccattttgtc gttactactg ttactttaga tggtaaggaa atagaaatgc
     4921 cagatcaaac gattagcgga ttgtttaatt actttaacaa ataaatattt attccgaaaa
     4981 gtgaaaaaac atat
//
LOCUS       DADQRX010000139         4931 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.145, whole genome shotgun sequence.
ACCESSION   DADQRX010000139 DADQRX010000000
VERSION     DADQRX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.145"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..970
                     /locus_tag="J8V14_002603"
     CDS             83..970
                     /locus_tag="J8V14_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBA0922671.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="J8V14_002604"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="J8V14_002604"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBA0922672.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="J8V14_002605"
     CDS             2272..2718
                     /locus_tag="J8V14_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBA0922673.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="J8V14_002606"
     CDS             2991..4343
                     /locus_tag="J8V14_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HBA0922674.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="J8V14_002607"
     CDS             4514..4801
                     /locus_tag="J8V14_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922675.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DADQRX010000140         4871 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.146, whole genome shotgun sequence.
ACCESSION   DADQRX010000140 DADQRX010000000
VERSION     DADQRX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.146"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="J8V14_002608"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="J8V14_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBA0922676.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="J8V14_002609"
     CDS             724..1878
                     /locus_tag="J8V14_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBA0922677.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="J8V14_002610"
     CDS             2093..3061
                     /locus_tag="J8V14_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBA0922678.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="J8V14_002611"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="J8V14_002611"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBA0922679.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="J8V14_002612"
     CDS             4091..4699
                     /locus_tag="J8V14_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBA0922680.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="J8V14_002613"
     CDS             complement(4779..>4871)
                     /locus_tag="J8V14_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBA0922681.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DADQRX010000141         4797 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.149, whole genome shotgun sequence.
ACCESSION   DADQRX010000141 DADQRX010000000
VERSION     DADQRX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.149"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..579
                     /locus_tag="J8V14_002614"
     CDS             <1..579
                     /locus_tag="J8V14_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBA0922682.1"
                     /translation="KEKYHLKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQ
                     PIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGII
                     EAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIY
                     GDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            552..1229
                     /locus_tag="J8V14_002615"
     CDS             552..1229
                     /locus_tag="J8V14_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA0922683.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1219..2361
                     /locus_tag="J8V14_002616"
     CDS             1219..2361
                     /locus_tag="J8V14_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA0922684.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2374..3351
                     /locus_tag="J8V14_002617"
     CDS             2374..3351
                     /locus_tag="J8V14_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0922685.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3351..4259
                     /locus_tag="J8V14_002618"
     CDS             3351..4259
                     /locus_tag="J8V14_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBA0922686.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4260..>4797
                     /locus_tag="J8V14_002619"
     CDS             4260..>4797
                     /locus_tag="J8V14_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBA0922687.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVF"
BASE COUNT         1819 a          584 c          882 g         1512 t
ORIGIN      
        1 aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt atttcatcca
       61 gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa ttcaatggaa
      121 caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag cgaaattatc
      181 agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa tttagagccg
      241 gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc ttcttctggg
      301 ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag acaaaatgga
      361 cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat tgaaactgct
      421 ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa gaacatctac
      481 ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt gaatgatggg
      541 gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt gatccataca
      601 ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga ttagaaaata
      661 agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg aaatatcttg
      721 ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat aaatttattg
      781 tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat atgccaagtg
      841 agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt tcaattggtt
      901 atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg
      961 ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac tatgttcatt
     1021 tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact caaataggaa
     1081 ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga
     1141 gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca gctaaaatta
     1201 agaaagaagg aattaatttt gaattctaga attttattat caagtcctca tatgggagga
     1261 acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc attaggtgaa
     1321 aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg cggagcttct
     1381 gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa tgttggaaaa
     1441 aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc tattttatat
     1501 caaggggctg taccagtgtt cattgactca gaacctaata catggaatat gagccctgtt
     1561 gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa ggcaatcatc
     1621 gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat tgcaaaccaa
     1681 tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta taagggaatt
     1741 tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa gatcatcaca
     1801 acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca ttcaagattt
     1861 ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat aggttataat
     1921 taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt attgaatgat
     1981 agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc tggaattgat
     2041 agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt gtctacttta
     2101 acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa actaaatgaa
     2161 aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt atttcaaggg
     2221 gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt taatagcggc
     2281 ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt tattgatgag
     2341 tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat tggtttaggt
     2401 tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat tttactatgt
     2461 ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg aataccaact
     2521 tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt tgtttgtaca
     2581 tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg taatatcttt
     2641 acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc taaaaagaaa
     2701 gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca atatattaag
     2761 tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca gtatttaccc
     2821 gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa aactaatggg
     2881 tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg gaaagttaaa
     2941 tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc ggacagtttc
     3001 actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga tgttgtttca
     3061 agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga atgggaaggt
     3121 aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga tattcaattg
     3181 tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga tgcatattta
     3241 gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca atatactttt
     3301 gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta atgatgaaag
     3361 taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta ataagctatg
     3421 gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga aaaaaagagt
     3481 ttgaagaaat aacgtatatt caaaatagag acgatttgga tacacattat gatatagctt
     3541 ttattacaaa tccgactttt aagcatctgg aaacactaaa tgtaattgtt gaaaaagttg
     3601 attacatatt tttagaaaaa cctgcttttt ctgagagtat tgatcttact caatataatt
     3661 atgattacaa aaatatttat gttgctgctc cactgaggta taaaaatatc atggaagttg
     3721 ttcgtaatag tattcaaagt aaaaaagtat attctgtaag agctatttgt tctacttatc
     3781 ttcctgattg gagaaaaggt actgattata gaaataatta ttctgccttt aaatctatgg
     3841 ggggaggagt agaattagac tgtatacatg aattggatta tattataaac ctatttggtt
     3901 tccctaaatc agtaaaaaaa cagtttggta aatattcgaa tcttgaaatt aacagtaatg
     3961 atttagcaac atatatattg agatacgatg ataaaattat agaaattcat ttagattatt
     4021 ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt atcaatgttg
     4081 acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg cctgaatctg
     4141 ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta acaggcaaag
     4201 aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag gagaaataaa
     4261 tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt
     4321 tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata gatatctatc
     4381 aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt aatgagttag
     4441 tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa cctgaattag
     4501 ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa ctagaaagag
     4561 aagataatat atttgattta attatagatt tagatttgac ttcaccttta cgtgaagttt
     4621 ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac gtctactttt
     4681 cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt gaaaatgaag
     4741 ttactatagt taataaatca agatacacat ctaggcaaca gacacctgaa gtttttg
//
LOCUS       DADQRX010000142         4541 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.150, whole genome shotgun sequence.
ACCESSION   DADQRX010000142 DADQRX010000000
VERSION     DADQRX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.150"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1306)
                     /locus_tag="J8V14_002620"
     CDS             complement(1016..1306)
                     /locus_tag="J8V14_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922688.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1648..1944
                     /locus_tag="J8V14_002621"
     CDS             1648..1944
                     /locus_tag="J8V14_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922689.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="J8V14_002622"
     CDS             1951..3903
                     /locus_tag="J8V14_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBA0922690.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="J8V14_002623"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="J8V14_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBA0922691.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1555 a          798 c          750 g         1438 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat t
//
LOCUS       DADQRX010000143         4526 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.151, whole genome shotgun sequence.
ACCESSION   DADQRX010000143 DADQRX010000000
VERSION     DADQRX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.151"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(635..2140)
                     /locus_tag="J8V14_002624"
     CDS             complement(635..2140)
                     /locus_tag="J8V14_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0922692.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2137..2823)
                     /locus_tag="J8V14_002625"
     CDS             complement(2137..2823)
                     /locus_tag="J8V14_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0922693.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3016..4437)
                     /gene="gndA"
                     /locus_tag="J8V14_002626"
     CDS             complement(3016..4437)
                     /gene="gndA"
                     /locus_tag="J8V14_002626"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBA0922694.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1262 a          934 c          745 g         1585 t
ORIGIN      
        1 cttgcttgtt aaaaatgttt gttgtcttga attgaatcaa gacgccctac acatttggtt
       61 tgtcttactt tgttcagttt tcaaaggtct aatctttttg ttttaacaac tctttaatct
      121 tatcagattc agaagagaat gtcaacaact tctttgttta ttttttctaa acgtctttgc
      181 cactcattag cagcgacttg attatcatac catctttatt gttatttgtc aataactttt
      241 tcgaagtttt tttcgataaa atcaagcttt attagctatc tcatccaaac gacttcgtta
      301 ttataacatg acatcaaaag tgcgtcaaca ctatttttta taatttttag tgacacctta
      361 aaggaatcat ttagaacaaa taataatata gcaatctctt tataaaattg caatagaaaa
      421 ttccctattt ttatttatta tttttaaaca cgaatattac agcattttat tgttttttcg
      481 ttcatctaaa agatacttac atttaattaa cggatgttat tctatttaat tgttcgctag
      541 tttttttata atgacatccc tgaatttctc acttgcttgt attttgtacc ttattctttg
      601 ttggatatat aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag
      661 gaataaagat tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt
      721 tatagttttc aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc
      781 tagctcttgc attatccact cgataaaaac gattgaagat cttattaagg tcttctttgg
      841 gtattccttc gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag
      901 agatatggac ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga
      961 ttaattgttc aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat
     1021 ctaaagtgat cacatagtct ggatataata tcttgaaatt attataaact tgataggtga
     1081 cttcttttcc tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata
     1141 agtctagcat ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta
     1201 atgattcttc taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc
     1261 cttcaataat tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt
     1321 gttgttcaat atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg
     1381 ccaattcatc atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta
     1441 tagtatccat tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga
     1501 aacctaaaat actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata
     1561 gcttatttcg aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct
     1621 tttctcttgt acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta
     1681 gtgtaacgat cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg
     1741 ttttaaaaat caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta
     1801 gctcagctat aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta
     1861 cgacctcatc tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca
     1921 gttcttcatc cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt
     1981 ttctttcttt tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg
     2041 taaacacaac gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc
     2101 ctttcagttc tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt
     2161 tccgcgtaca gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata
     2221 acgaatatat acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt
     2281 tagtaaaacg tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc
     2341 gtattctctt tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt
     2401 ttcgatagtc aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc
     2461 aatatcgatt cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg
     2521 tttgacgatg tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc
     2581 tcttgcagtc atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc
     2641 taaaccgttc aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa
     2701 tgccgcatct aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt
     2761 caactctagc tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct
     2821 catttattat tttatccttt ctattctact ggctttttaa aaactctatc attataccga
     2881 taatatgctt aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga
     2941 agtctactca taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta
     3001 acagccgaaa gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc
     3061 gacacgctca taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg
     3121 cagacgttct gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg
     3181 tacgccagct tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt
     3241 gatttcaacg aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc
     3301 atccgtaatt ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc
     3361 gatttcacca aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa
     3421 tccttgggca taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa
     3481 ttcttttttg tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg
     3541 tacgcgttct tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg
     3601 aagtggcaca cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc
     3661 agctgcatca aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt
     3721 cagaatgtca gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt
     3781 aaagatatca gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga
     3841 ttcagcaatc agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg
     3901 tccggctcca tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga
     3961 aatttgctct aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat
     4021 tgaaggtcct tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat
     4081 gccagagttg gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc
     4141 gtcaatcaaa atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt
     4201 tgccggtcct gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa
     4261 ttcttcgatt gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt
     4321 tgttttagcc cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa
     4381 ggctagattt ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg
     4441 aactcctcct acatacggga tttctcccta atctcttaca ttatagctta acacggctac
     4501 attgcaaact aaagaacaac atattt
//
LOCUS       DADQRX010000144         4495 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.152, whole genome shotgun sequence.
ACCESSION   DADQRX010000144 DADQRX010000000
VERSION     DADQRX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.152"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..531
                     /locus_tag="J8V14_002627"
     CDS             <1..531
                     /locus_tag="J8V14_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922695.1"
                     /translation="DENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDI
                     FAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIS
                     TISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILL
                     PENWAELRKLVEYGII"
     gene            518..1939
                     /locus_tag="J8V14_002628"
     CDS             518..1939
                     /locus_tag="J8V14_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBA0922696.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1952..2959
                     /locus_tag="J8V14_002629"
     CDS             1952..2959
                     /locus_tag="J8V14_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBA0922697.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2971..3975
                     /gene="neuB"
                     /locus_tag="J8V14_002630"
     CDS             2971..3975
                     /gene="neuB"
                     /locus_tag="J8V14_002630"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBA0922698.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3972..>4495
                     /locus_tag="J8V14_002631"
     CDS             3972..>4495
                     /locus_tag="J8V14_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBA0922699.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT         1575 a          575 c          812 g         1533 t
ORIGIN      
        1 gatgaaaaca gtaaattgat gaaaaacata caatcaatct ttgggataga agaagttttt
       61 gtcggagata aatgtatctt ttttgatcaa cctttattat ctgatggtta tggcatagat
      121 gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga aactttaatt
      181 aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt gaattccagt
      241 ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt gattagtcct
      301 atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa ctctattatg
      361 ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa agaaacattg
      421 acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa aattttactc
      481 ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta acacactttc
      541 caagtcaaaa ttagttaagt ccagtttctg gtatactata gggaattttt ttattaaggg
      601 aatttcattc ataactattc ctatttttgt aaatattact actcaagcag aatatggttt
      661 gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc taagcctaaa
      721 ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat ttatgtcatc
      781 ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga acatatattt
      841 tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga tgttattaca
      901 aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta actttaacca
      961 ttttaaatat ttgattttat cttttatatc tacttttttg aatattgcaa tctctgtagc
     1021 tttgatgcag acattatatt ttgatgataa atatgttggc agagtagtcg gcggaacact
     1081 gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg gtgctcattt
     1141 ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa tcccacattc
     1201 cttagcaaat atagttttag cacaattcga tagaattatg attaattctt atttaggtcc
     1261 tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa gcgtaatatg
     1321 gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta aaaataatga
     1381 aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga ttacgatgat
     1441 tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact attattctgg
     1501 gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat atagtttacc
     1561 agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggta cagtagcaag
     1621 tgctatagtt aatatactat taaacatgat tttcatacct aggttaggaa taattgctgc
     1681 tggatacaca accgtattta cgtatttttt actttttatt tttcattata atatcgctaa
     1741 aaaaattacg aataaacaat tatttgacac aaaaaaaata tgtatcataa taatttttgt
     1801 atcaattttt tctttgatac ttcatatttt aatcaatcaa atgttgattc gttacgtttt
     1861 gacatcatta ttgataatta gtttagttat atatttttta aagattaaga atcgaattat
     1921 agatgactat aattcatagg aggagatata agtgtttaaa ggaaaaacaa tattagtaac
     1981 tggcggaact ggttccattg gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa
     2041 agctatcaga atttatagta gaggtgaaga aaaacaattt tatatgcaac aagagctctc
     2101 aaatcacact aatttgagat ttttaattgg tgatatcaga gactctcaaa ggattgatta
     2161 tgcatgtcgt gatgtggata ttatttttca tgctgccgca atgaaacacg ttccagcaag
     2221 tgaatataat cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga
     2281 tgcagcgatc aaaaataatg taaaacattt tgtaggtatc agtacagata aagtagtgag
     2341 tcctactaac accatgggtg cgacaaagtt attgagtgaa aaattaataa tagctgccaa
     2401 tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa atgtcatggg
     2461 gtcaagtgga tctgttattc ctctttttag taaacaaata aaaaacaata aagatattac
     2521 tttgacagat gttgatatga cacgatttat gatgtcaata ccggatgctg caagattagt
     2581 tctgtctgct gctatgaatt caaaatttgg agaaacatat gtcttaaaaa tgccggcaat
     2641 caatatttta actttagctc aatcattaat aaaaatatat aatgatacac atgatgaaaa
     2701 atatagaggt gaaataaaag ttgtaggtat caggccagga gaaaaattat atgaagagct
     2761 aatgactaca gacgaaattg aacgctcata tgaaaatgat aaaatgtata tcattccacc
     2821 acactatcat gctactgacg aactgtacaa agattatgaa aaaattaaaa tcactgcata
     2881 ctcttcagat gcaacagaat taatgagtgg acaggaattt attagaatcg caaaagagaa
     2941 taatgtcgtg acttggtaag gagttttatg atgaaatcag aagtatatgt aattgcagaa
     3001 attggtgtaa accataatgg aagtctagat ttagctttag aaagtattga taaagctaaa
     3061 gaatgtggag ctaacgcggt taaatttcaa acatttaaaa cgaataagct agtttccaaa
     3121 aaggctccaa tggcaaaata tcagaaggat aatcttaaag aggacagttc tcaatttgaa
     3181 atgctaaaaa aattagaatt atctcaatct gattttatta gaataaaaga ttattgtata
     3241 caaaataaaa ttgatttttt gtctactcct tttgatgaag aaagtgcaga ctttctaaaa
     3301 caaattggtg tacaaggatt taaaattggc tcaggagatt taacaaattt accttttctt
     3361 agaaaaatag atatgtatgg gttgccagtt ttattatcta caggtatggc tgatattcat
     3421 gaggtcagtg aggctgtaga ttgtttcata gatagtccag ttactatttt acattgtact
     3481 tcaaattatc cggctagttc tgaagatatt aatttattgg cgattaacac attaaaagaa
     3541 atgttcggga tacccgttgg gtactccgat cattcattgg gatatgatgt tggtatttgt
     3601 gctgttgcga tgggtgctaa ggtaattgag aaacatttta ccttggataa ggaactacct
     3661 ggtccagatc ataaagcttc tttagatcct caggaatttg cagattttgt aaagcatatt
     3721 agaaacactg aaatattttt aggtgatggt gttaagcgag caatgccttc tgaacttagt
     3781 acaaaagaag tatcaagaaa aagcctagtg gtcactgaga atttaaatgt gggagatatt
     3841 cttacagaga aatctcttac aataaaacgt cctggaaatg gaatagctcc taagttttta
     3901 tatcaatata ttggcaaaaa agttaaaaga ccaataagtg cagatagtgt tctgcttgag
     3961 gaagatgttg aatgaaaaaa atacttatag taagtggaac acgagctgat tatggaatat
     4021 ataaacctgt tgctgagaat ttactacaag ataattggga tgtatcattt gttgtttctg
     4081 gaatgcattt atcccataaa tatggattta ctaaagaatt gatcttagct gacggattta
     4141 gaattagtgg agaagttcaa agtttgtttt tagagaacaa taagggaaac atggctcggt
     4201 ctgtggcatt agagataaat ggtttcacaa atatatttga acaaactagc cctgattttg
     4261 tattattact aggggataga ggagaaatgt tggcagctgc gatagcttgt ttgtatcttg
     4321 gaatcaaaac agcacatatt cacggaggcg aagtgtctgg tactgtagat gaatcaataa
     4381 gacatgccat aacgaaaata gcatctgttc attttgctgc tacggaaaaa agcaaagagc
     4441 gtttaataaa aatgggggaa gattcttgga gagtttttcc cgtcggagct cctag
//
LOCUS       DADQRX010000145         4486 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.153, whole genome shotgun sequence.
ACCESSION   DADQRX010000145 DADQRX010000000
VERSION     DADQRX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.153"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(339..869)
                     /locus_tag="J8V14_002632"
     CDS             complement(339..869)
                     /locus_tag="J8V14_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBA0922700.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            974..1525
                     /locus_tag="J8V14_002633"
     CDS             974..1525
                     /locus_tag="J8V14_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA0922701.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1678..2151)
                     /locus_tag="J8V14_002634"
     CDS             complement(1678..2151)
                     /locus_tag="J8V14_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA0922702.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2289..3761
                     /locus_tag="J8V14_002635"
     CDS             2289..3761
                     /locus_tag="J8V14_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBA0922703.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3758..4468
                     /locus_tag="J8V14_002636"
     CDS             3758..4468
                     /locus_tag="J8V14_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBA0922704.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1410 a          818 c          860 g         1398 t
ORIGIN      
        1 gctcaaaaaa cggcaaaata tgaggaacta tttttccatt tgtttctcta atactcaggt
       61 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
      121 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
      181 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
      241 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
      301 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
      361 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
      421 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
      481 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
      541 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
      601 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
      661 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
      721 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
      781 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
      841 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
      901 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
      961 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
     1021 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
     1081 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
     1141 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
     1201 agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt
     1261 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
     1321 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
     1381 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
     1441 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
     1501 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
     1561 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
     1621 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
     1681 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
     1741 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
     1801 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
     1861 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
     1921 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
     1981 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
     2041 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
     2101 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
     2161 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
     2221 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
     2281 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
     2341 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
     2401 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
     2461 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
     2521 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
     2581 ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt
     2641 gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
     2701 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
     2761 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
     2821 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
     2881 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
     2941 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
     3001 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
     3061 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
     3121 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
     3181 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
     3241 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
     3301 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
     3361 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
     3421 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
     3481 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
     3541 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
     3601 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
     3661 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
     3721 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
     3781 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
     3841 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
     3901 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
     3961 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
     4021 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
     4081 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
     4141 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
     4201 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
     4261 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
     4321 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
     4381 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
     4441 attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgta
//
LOCUS       DADQRX010000146         4485 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.154, whole genome shotgun sequence.
ACCESSION   DADQRX010000146 DADQRX010000000
VERSION     DADQRX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.154"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..686
                     /locus_tag="J8V14_002637"
                     /pseudo
     CDS             78..686
                     /locus_tag="J8V14_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="J8V14_002638"
     CDS             706..1734
                     /locus_tag="J8V14_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBA0922705.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="J8V14_002639"
     CDS             1731..2489
                     /locus_tag="J8V14_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBA0922706.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="J8V14_002640"
     CDS             complement(2575..3315)
                     /locus_tag="J8V14_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0922707.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..>4485
                     /locus_tag="J8V14_002641"
     CDS             3376..>4485
                     /locus_tag="J8V14_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBA0922708.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKK"
BASE COUNT         1498 a          784 c          858 g         1345 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaa
//
LOCUS       DADQRX010000147         4444 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.155, whole genome shotgun sequence.
ACCESSION   DADQRX010000147 DADQRX010000000
VERSION     DADQRX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.155"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..884)
                     /locus_tag="J8V14_002642"
     CDS             complement(459..884)
                     /locus_tag="J8V14_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922709.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1258..2229
                     /locus_tag="J8V14_002643"
     CDS             1258..2229
                     /locus_tag="J8V14_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0922710.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2261..3658
                     /locus_tag="J8V14_002644"
     CDS             2261..3658
                     /locus_tag="J8V14_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA0922711.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3817..4440
                     /locus_tag="J8V14_002645"
     CDS             3817..4440
                     /locus_tag="J8V14_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922712.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1556 a          714 c          904 g         1270 t
ORIGIN      
        1 aattactacg tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt
       61 tgaaataaat aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta
      121 ctgatgaagc ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt
      181 gagttttgct tggatttgtt tatcttcttc tactttttac ataattttga acaggataag
      241 attcaaatgt tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc
      301 ttccttggtc attaaattct gtatggatat ttttgttgtt attacatgta caataatcag
      361 gactatcgat catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa
      421 aagaagggga gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca
      481 tcctcatcct tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta
      541 ttttcttttc ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc
      601 actgtttttt cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc
      661 cattccattt ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt
      721 acttttagcg tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa
      781 tcggttgttt cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc
      841 aaaagtcccg cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt
      901 gataggataa agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa
      961 cgcacaaaac acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat
     1021 ccgccacttt cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt
     1081 aaaaatgaac cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct
     1141 actgaagact ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc
     1201 atcataatcg aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg
     1261 gcatcgatcc gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac
     1321 ttgaatggtc agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg
     1381 ttggactata aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt
     1441 ggacttttga taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa
     1501 agagtagctg aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa
     1561 aaaattgaac aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc
     1621 ttatccgaag aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc
     1681 gcattgaatt gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa
     1741 agtgtagcaa aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt
     1801 gctgccactc aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc
     1861 caaaatgatc catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc
     1921 tattacgggg caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac
     1981 aatatgtcga ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat
     2041 attgcgttca gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc
     2101 acgatcacgc caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga
     2161 atcaaaaaag aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga
     2221 atcaactaaa acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc
     2281 agcaaatttt tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg
     2341 cgatggaaaa ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt
     2401 tgatcaagta ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca
     2461 gttgatgaaa cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat
     2521 acctgatgaa tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga
     2581 tgagttgatt gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat
     2641 gtatctgcaa gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata
     2701 tgccaaaagt tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga
     2761 accaatcgtt ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga
     2821 tatgaaaaaa gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa
     2881 atcttaccat gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag
     2941 ctatccaaga gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt
     3001 cttcaatcgt tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga
     3061 gatagtaaaa gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa
     3121 agaaaataca gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa
     3181 agaaacacca atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga
     3241 aatgcctggt cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta
     3301 tgatattttg atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa
     3361 cggtatgggt gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga
     3421 ttatcggatc gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg
     3481 tgccaattgt ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc
     3541 atataagaat cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat
     3601 caaaaaatcg ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga
     3661 aagaatagtt gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag
     3721 ttcaattatt tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa
     3781 tagaatagtc aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt
     3841 ctcttttggt tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa
     3901 atgacagcag atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta
     3961 gataatttca aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat
     4021 tacgaaaaag gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt
     4081 aatatcgatg acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat
     4141 ttgaccgttt tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa
     4201 gtgatcaccg cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa
     4261 tggaaaaaga caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg
     4321 acaggaacaa gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat
     4381 caagcctcaa ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga
     4441 aaaa
//
LOCUS       DADQRX010000148         4425 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.156, whole genome shotgun sequence.
ACCESSION   DADQRX010000148 DADQRX010000000
VERSION     DADQRX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.156"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="J8V14_002646"
     CDS             690..1658
                     /locus_tag="J8V14_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA0922713.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="J8V14_002647"
     CDS             1669..2484
                     /locus_tag="J8V14_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBA0922714.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="J8V14_002648"
     CDS             2552..3262
                     /locus_tag="J8V14_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922715.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="J8V14_002649"
     CDS             3298..>4425
                     /locus_tag="J8V14_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBA0922716.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DADQRX010000149         4403 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.157, whole genome shotgun sequence.
ACCESSION   DADQRX010000149 DADQRX010000000
VERSION     DADQRX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.157"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..707
                     /locus_tag="J8V14_002650"
     CDS             <1..707
                     /locus_tag="J8V14_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBA0922717.1"
                     /translation="IVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEG
                     IRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAV
                     CVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQL
                     SEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKSVIIYMTFCL
                     KRRLSIKRPFGLNPCI"
     gene            698..1483
                     /locus_tag="J8V14_002651"
     CDS             698..1483
                     /locus_tag="J8V14_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBA0922718.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            1452..2168
                     /locus_tag="J8V14_002652"
     CDS             1452..2168
                     /locus_tag="J8V14_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBA0922719.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2270..3289)
                     /locus_tag="J8V14_002653"
     CDS             complement(2270..3289)
                     /locus_tag="J8V14_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBA0922720.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(3308..4366)
                     /locus_tag="J8V14_002654"
     CDS             complement(3308..4366)
                     /locus_tag="J8V14_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBA0922721.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         1462 a          868 c          793 g         1280 t
ORIGIN      
        1 aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac ccgacacatg
       61 cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt atcgttgtca
      121 tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt cttcctttct
      181 caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct gaaaaagaag
      241 gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa gagattattg
      301 gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa
      361 aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc gccgtttttc
      421 ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata gcagaacagt
      481 taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc atctcttctg
      541 ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa acagagcagc
      601 tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt tgtttaaaga
      661 gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg ggatagaaac
      721 tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt
      781 acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga aacaatatat
      841 aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca tttctggttt
      901 gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa atcaaaatta
      961 taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg ccggtttttt
     1021 attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag aaagcgattc
     1081 ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag tgttagggaa
     1141 cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg gacaggtccg
     1201 gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct gcgatgacgg
     1261 tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt cgacgaaggg
     1321 aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc acggtggatt
     1381 tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat taccaaaaaa
     1441 ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg gttgcagaat
     1501 tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt ttcagaaatg
     1561 tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat
     1621 acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa ggaaatgata
     1681 tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat gtgatccgct
     1741 acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag ctggcgatcg
     1801 aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat cagaaattga
     1861 tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc atcgatttgc
     1921 ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta aaagaagata
     1981 ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata tccgtcaaga
     2041 aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa tatttggaaa
     2101 tcggccggcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag ctcacagatt
     2161 actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa gtaacaactg
     2221 tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct tatcttaatt
     2281 gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt atttcacgaa
     2341 agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt gaacctacat
     2401 gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca gggtctggaa
     2461 tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg
     2521 ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca ttatcttgaa
     2581 gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct aaaaatcctt
     2641 cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg acaatatcgc
     2701 ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca gcttctgtct
     2761 gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca gcttccgtca
     2821 accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca cgactaaaca
     2881 tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac gcagtatttc
     2941 tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc gaatacgatg
     3001 gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata tgattgactc
     3061 ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca agattggtct
     3121 catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct gacacgatat
     3181 tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata ttcaattttt
     3241 caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata ctttcttcct
     3301 ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg atccatacag
     3361 gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct aaaagtgcct
     3421 ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta gccatcatga
     3481 taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc gtcaaaactg
     3541 ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg taataccatg
     3601 aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg ccgattgcta
     3661 gccacgccaa tggtgaactt aaaccagcta aataatccat cgactgaaat agccacggtg
     3721 cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca cctgtaactg
     3781 acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa
     3841 tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag ccactcattt
     3901 ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt gaggaataca
     3961 tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag gtcgtagaac
     4021 caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt aagaaaaaag
     4081 gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag acattgattc
     4141 cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca
     4201 aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa aaaatcccct
     4261 tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt gcactgacaa
     4321 tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt ctcctcctaa
     4381 ttatgttcat ttattcacaa taa
//
LOCUS       DADQRX010000150         4364 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.158, whole genome shotgun sequence.
ACCESSION   DADQRX010000150 DADQRX010000000
VERSION     DADQRX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.158"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..162)
                     /locus_tag="J8V14_002655"
     CDS             complement(<1..162)
                     /locus_tag="J8V14_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0922722.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQ"
     gene            complement(156..557)
                     /locus_tag="J8V14_002656"
     CDS             complement(156..557)
                     /locus_tag="J8V14_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBA0922723.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(744..2552)
                     /gene="uidA"
                     /locus_tag="J8V14_002657"
     CDS             complement(744..2552)
                     /gene="uidA"
                     /locus_tag="J8V14_002657"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBA0922724.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2575..4341)
                     /locus_tag="J8V14_002658"
     CDS             complement(2575..4341)
                     /locus_tag="J8V14_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBA0922725.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1358 a          877 c          553 g         1576 t
ORIGIN      
        1 ttgacctacc tcgttaaatc catttgtcaa acataactta gccatataag aatgataatg
       61 tttgagtatc atcttgatcg aaattggatc tccttctaga gcagataaga taatggatag
      121 tggaactaat aggtactgcc gctttttaga tttatctacc ataggtcata ccccattttc
      181 aattgttcaa ctgccttctt tcgttgatac cagataccac ttgtgctcat attgagaatt
      241 ttaccgattt cagcatcatt aaaacctgca aaataataaa gtaggataat ctttcttctt
      301 atctccgata actgatcaat tgctgctgat aacttttgat ctgaaattaa tatttccatt
      361 ccaaataaga aatagacatc ttgttcatca aaatgagttt gggaatacct gccttcattt
      421 gaatctgcca atggttcctc attatccttt atgttagcgt ttcgttttcg aatagactta
      481 gcttcgtttc gtaatacttt tttacagtag gcatcaaatt ggtgttgtat agatatgtca
      541 ttgtaatgtt gttgcatgat aatatgctcc tttttccccc tttccactaa tacaacaatc
      601 agaaagcccc tttttttcga aaaaactaga aaaaaaacat aaaaatattt atcattcttt
      661 aaacagaaaa tctttctata aaaaacaaaa aaaaacgatt aggctaggct aatcgttttt
      721 tgaacattct taaaaaatcc aaattatttt ttataactga aattgggtat tgatgaccat
      781 cgtttctgta gatagtaagc aatcatttta ggttttctat ctctagtaaa gattcctttc
      841 ttatttcctt gcactcttaa aatcccttga cttgttgcaa aatctgcaaa attccacact
      901 tgttcaccaa caaaattttc acatgtatct attacttcgt gatttgcttc gtaataagcc
      961 acttgatact cttctgtaaa cattgttggt actgtatcat gaaatccagc tactgtatct
     1021 gctccatatt ctgtaaacat tattggcgta tcaggacatc tttccatcca tccatctagc
     1081 tcttttttca acagatattt tgcatgtgcc agatcccctc ccgaaacata ccaaccataa
     1141 taacgattta aacataaaac atcgacaata tctcctacct tacaaacatc tggcgttgat
     1201 tttagataag taacgattgt tacaggtctt ttctgcggat caagttcttt agttaattca
     1261 actaaaggtt caaaatattc tttagcacct tcggaatccg aatctggttc atttgcaata
     1321 gaccacatta caacacttgg gtggttcttg tcgcgttcta tcaattcctt gatcaccgtt
     1381 ttatgagaat tttctgttcg tatctctttc cacgtattat gtttttccac attatcatct
     1441 aataaagtgg ccatgaaatt taaatgtaac cctactgcag gtacttcatc aataactaca
     1501 ataccttctc gatctgctaa tcgcataatt tcttctgagt aaggataatg tgatgttcta
     1561 aaagaattag cacctatcca tttcattaaa ttaaaatctt taatatttac agcctcattc
     1621 aaaccacgtc cattaacata cgaatcctca tgcttaccaa atcctttaaa ataaaaaggt
     1681 ttattattaa tcaaaaattt gccattggta acctgaacac tgcgaatacc aaattcttct
     1741 gtataggtat cgatcacttt attatcatca attaaactaa ttttcatact atataaataa
     1801 gcgtctagag gttcccaaaa aattggattc ttaagctgaa tcattccttt ctttccttca
     1861 gcttcagcta caagttgagc ctcttcgtca tatatcgcta ttaagatttt tccggtccat
     1921 tctgtatcga tattgaattc tacacttgca tccaaatcag ttattcttgt attaatagta
     1981 atatcactta tatagtcttt cggagttgta tataacttaa caggtctatg aatacctgca
     2041 taattgaaga aatcaaaatt aactaagttt tcctttttta tatccccatt ttcctgtact
     2101 gtttccttca taatccccac aggtaaagta ctatcgtcaa taatattgtt tacagcaact
     2161 gtaattcgat tattcccgtc ttttacaaac cgattaattt cagactcaaa tggtgtaaac
     2221 ccacctttgt gttgagtaat aaactttcca ttcaaaaaaa cttttgcttc atgtgttgca
     2281 gatccaaaac gaagaactaa tctttcttct aataatcttt taggaagagt cacttctttt
     2341 tcataccaca cccatccaac atgatttcta atgttctctg tttcaaataa atcattataa
     2401 gaagagggaa ctgaaatcgg ataactctct ttttcattta ttggttctaa agcaagttct
     2461 tcctgtaagt catcatttgt atctaattta aatctccaga gaccgcttaa gtcaattact
     2521 tgcctcgtct ctgtcataat gggatacaac atataaacca ctcctatcta aatattaaat
     2581 aaatgtatcc tttaaaccac aataagcatc tattggactt actcccttaa attcagaaag
     2641 acccattagt ttgtccacca atatttctaa agtttcttgt tttccatcat aagtattaat
     2701 ataagtcttt acttgaggta catcagccaa atgaaacgga cactgtacag atacaaaaat
     2761 tgttggaatt tcatgaatgt aaaacggtat atccggagtt cctttacttg ctggccagac
     2821 aattcgttga actgtgcctg gattaacatt agctatatta atgatcaagt catatttttt
     2881 agttaaattt ttaattggtc tcttagcagc atatattcca gctatatttt taattttttc
     2941 ttcttgcgat aatcctttca attcttcttc aggtgaaatg tatttttcaa cttcaaatcc
     3001 ttttgctgac aaaatctgtt tcaataaatc aatggcttga ggtctatttc cagcaactac
     3061 ttcaccaaac ccacctttta ccccatgtac atctactagt aaaatccttt tatatttttc
     3121 tggtacaata ggccaaatat cttgtttatc tttaactaat gtaattgctt ggtcagccac
     3181 ctctttaaaa attgatttat tttcaattaa accaattttt tccagagctt cttgcttagg
     3241 taataaaatt tgttcttttg cttttttgtg taatcccaat ttagctttta accctaaaat
     3301 tctttttaag gcatctaata accgatcatc agtgataatt cctttacgat agccttccat
     3361 catataatta aagtcttcat ctggatcatt aaaaaataga aatagatcac atccagctgc
     3421 aatagaagtg ggcaataatt ctgatcgttt cattgcccca gtcattccta acatatgact
     3481 agcatcagta actactaatc cattaaatcc gagttttcct ctaagtaagt cagtgattaa
     3541 ttcttttgat aaagttgcag gaagtaaatc ttcatctttc agatcaggat taaaatgttt
     3601 tgaataagct ggcatatgga tatgacctgc cataattgaa ggtaatccag cttctattaa
     3661 gcctgagtat acttttccaa aagaattatc ccattcttcc gtacctaaac tattaatact
     3721 aaaagataga tgctgatctc gttcgtcaat accgtcacca gggaaatgtt tagctgcagg
     3781 caaaattcca ctctcctgaa tacctttcat ataagctaaa ctaagttcta aaactttatc
     3841 agggttttgt ccaaaagatc gactagaaat gataggattt ctccaatttc tattaatatc
     3901 tacaatagga gcaaaactcc agttacatcc aatagccgat gcttcaatcc cagaaactct
     3961 ccctaattca taagcatatt tcgcatcatt tgttgcacca atttttactt ctacaccaac
     4021 ttcagtacca tctgtacaag ctccgttacc acctgcttcc gtattagcag caattaatag
     4081 aggaatttta ctttctgttt gtaagatata gttttgatca tatacctcat cagcagttcc
     4141 tggattatat cgtacagctc caatatggta ctgattgacc atattactca agtaattttt
     4201 atcacgacta gatcccatat taacaaacaa ttgacctatt ttttcttcca aagtcataga
     4261 atcaatcgtc tcttcaatcc aagaaatagc atcttcatct aaattatatg gtttttttcg
     4321 taaatctact tttatagtca ttttctttct cctctactta ttag
//
LOCUS       DADQRX010000151         4330 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.159, whole genome shotgun sequence.
ACCESSION   DADQRX010000151 DADQRX010000000
VERSION     DADQRX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.159"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..792
                     /locus_tag="J8V14_002659"
     CDS             <1..792
                     /locus_tag="J8V14_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA0922726.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="J8V14_002660"
     CDS             889..3138
                     /locus_tag="J8V14_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBA0922727.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="J8V14_002661"
     CDS             3141..4316
                     /locus_tag="J8V14_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA0922728.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1511 a          616 c          796 g         1407 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa
//
LOCUS       DADQRX010000152         4328 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.160, whole genome shotgun sequence.
ACCESSION   DADQRX010000152 DADQRX010000000
VERSION     DADQRX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.160"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..1372
                     /locus_tag="J8V14_002662"
     CDS             join(33..474,474..1372)
                     /locus_tag="J8V14_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBA0922729.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            1526..2380
                     /locus_tag="J8V14_002663"
     CDS             1526..2380
                     /locus_tag="J8V14_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBA0922730.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIVHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
     gene            2472..2687
                     /locus_tag="J8V14_002664"
     CDS             2472..2687
                     /locus_tag="J8V14_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBA0922731.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2705..2977
                     /locus_tag="J8V14_002665"
     CDS             2705..2977
                     /locus_tag="J8V14_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBA0922732.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2979..3842
                     /locus_tag="J8V14_002666"
     CDS             2979..3842
                     /locus_tag="J8V14_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBA0922733.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1743 a          645 c          754 g         1186 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttcataaag agggctttta tatattaatc acaaatcact tatcacaaat
     1441 cacaagtgat taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta
     1501 tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt
     1561 aagacgtatt ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg
     1621 tggtgttgga aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt
     1681 aaaagtttta atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac
     1741 ttttgaggta gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc
     1801 ttcttctatt gttcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat
     1861 gttactgcca aaattaactc gctcatggac ttttgaaaat gaaagtagat tgcttgctac
     1921 tcttttagca cctttaaaaa gtgactatga tctcattatt attgatactg taccaacgcc
     1981 aagcgtttat acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc
     2041 agaagaagaa agtacaaaca acattcaaaa ctatatttcc tatttgattg atttacaaga
     2101 acaatttaac cctggactag atatgatcgg ttttgttcct tatttagttg atacggacag
     2161 cgcaacgata aaatcaaacc tggaagaact gtacaagcaa cataaagaag ataacttggt
     2221 tttccaaaat attatcaagc gaagtaataa agtaagtacc tggtctaaaa atggaattac
     2281 agaacacaaa ggctatgaca aaaaagtttt atccatgtat gagaacgtat tttttgaaat
     2341 gcttgagcga attattcaat tagaaaacga aaaagaatag aatcacaaat cacaagtgat
     2401 taatcacaaa tcacttgtga tttgtgattg ttgatgataa aataagaata agaagaaata
     2461 gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agcaaaacga aatgatacac
     2521 caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt gctgacttac
     2581 accatattat aaaaattgaa acagcaaaaa atggcggaaa cgtaaaagaa gttatggatc
     2641 aagccttaga agaatatata cggaaatatt tacctgataa actttaaaaa ggagtgaaaa
     2701 tgaaatggca gttacgtatg aaaaaacatt tgaaatagag atcattaacg aattatcggc
     2761 aagcgtttat aatcgagtat taaactatgt tttgaaccat gaactagata ctaaaaatac
     2821 tcgtttacta gaagtgaatc ttttaaatca attagaagtg gcacaagaag ttgatttatt
     2881 tcaacaacca tttgaagaat tacaagctat tcatgagtat tggcggtcaa tgaatcaata
     2941 ttcaaaacaa attttgacaa aagagaaagt ggcttaacat ggcgaatatt actgacttca
     3001 ccgaaaagca atttgaagat cgtttagaaa agaatgttga acgactaact aaaaatagac
     3061 tagcggttga atcgccaacc gcttttttac ttggtgggca accaggatca ggtaaaacta
     3121 gtttgcgatc ggcaatttcc gaagagacac aaggaaatgt tgttatcatt gataatgata
     3181 cgttcaaaca acagcaccct aattttgatg aactagtgaa actttatgaa aaagacgtag
     3241 taaaatacgt taccccttat tctaatcgca tgacagaagc gatcataagc cgtttgagag
     3301 ataaagggta taatttagtg attgaaggta caggacgaac aacagacgtt cctattcaaa
     3361 ccgcaacaat gcttcaagcc aaagattatg aaacaaaaat gtatgtcatg gcagtaccta
     3421 aaatcaactc atatttagga acaattgaac ggtatgaaac tatgtatgca gatgatccaa
     3481 tgacagccag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
     3541 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag
     3601 gagtgaaact ctattcaagc ttagaaacgc cttccattag tccgaaagag accttagaaa
     3661 gagaattaaa tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc
     3721 aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa
     3781 tggaaagctt gcagccacct acaccaccaa tacccaaaac acctaaactt ccagggattt
     3841 aaaccttaaa aaagtaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
     3901 aacatttcct cattttttaa aagctgatcg agaaataaag cacacaactg tattctttct
     3961 tttttcagtt ggtacatttt gcagtctgta acgtgccatt tactgtttgt taccttaaca
     4021 tatccagtcg aaaacacaag ggtatataaa cgagcaagct cgcccttgtg ttttctttgt
     4081 gtctatgttg cgttctcgtc acagcaaacg acacgaacac actggcgaaa atgaaccaac
     4141 aaaacgaaaa aagaaaggag cgtacactgt aatttatttc tagcttttaa aacatgagga
     4201 aaacagcttc acttcctcaa acgcctaaca aaaatggttg tcaaaaaact aaaaatcaaa
     4261 aaaagaaaag aggaaaagaa aatggaaaaa acaaaaaaat tacaattaga agattttaca
     4321 gaaaatgg
//
LOCUS       DADQRX010000153         4299 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.161, whole genome shotgun sequence.
ACCESSION   DADQRX010000153 DADQRX010000000
VERSION     DADQRX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.161"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(144..881)
                     /locus_tag="J8V14_002667"
     CDS             complement(144..881)
                     /locus_tag="J8V14_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBA0922734.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(1034..2374)
                     /locus_tag="J8V14_002668"
     CDS             complement(1034..2374)
                     /locus_tag="J8V14_002668"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBA0922735.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(2611..4134)
                     /locus_tag="J8V14_002669"
     CDS             complement(2611..4134)
                     /locus_tag="J8V14_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBA0922736.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         1263 a          946 c          691 g         1399 t
ORIGIN      
        1 cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa
       61 atcccgactt attttgaaag attcatttgc tatctatagt taattgacta gttttttcac
      121 ggaccctata ggacccattt atattatttc aactttttca gccagttttc caatgtattg
      181 acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca
      241 ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc
      301 ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc
      361 gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt
      421 gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt
      481 gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct
      541 ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata
      601 gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct
      661 acgagagcac caggataaaa aataagtaat ggagactttc catgattctt tttctctcct
      721 tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac
      781 gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa
      841 atgatgacta agccaattag tattcgtcta aatattttca ctttctccca ccttttctat
      901 ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat
      961 gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt
     1021 tgtatgttta tttttattta gcacataaac gatacacagc ttctgcataa atttccattg
     1081 ctttatacat actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat
     1141 cagggaacat cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc
     1201 ctgatacaaa cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt
     1261 tcactaactc actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag
     1321 tcaacccata tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg
     1381 taacagggat tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg
     1441 caaagttcat cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac
     1501 cagttgcatt ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt
     1561 ctgaaagagc aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt
     1621 ggatactttt accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt
     1681 ttttcaatgc ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa
     1741 caacattcaa cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt
     1801 aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc
     1861 cttgcgtaat tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt
     1921 catctgtacc aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag
     1981 ctttgacggc atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa
     2041 ctaaccagcc gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc
     2101 ctgctggaac aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct
     2161 ctgaataacc gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga
     2221 tttccaatac tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg
     2281 aatctgcttc atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt
     2341 gttcatgatg ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa
     2401 agggtcatgt caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc
     2461 tgctccatca gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat
     2521 ggagctattg acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat
     2581 gatagacctt gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac
     2641 acagatggct acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac
     2701 atcgaaacga gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt
     2761 tactaaacca gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa
     2821 ttcacccata ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat
     2881 caagaatgac atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa
     2941 tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat
     3001 aaccacttgg ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc
     3061 accagctaaa aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac
     3121 gatggacatt actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag
     3181 catgctttta cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga
     3241 ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat
     3301 accaaaagta ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg
     3361 acgaccgatt gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa
     3421 tgattttgtt ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat
     3481 agctaacaaa ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc
     3541 aacagcttgt gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac
     3601 ttgagaccct actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat
     3661 gactgggatc agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc
     3721 gcctaatgca aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt
     3781 cttcacaact gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa
     3841 tccaccaaca accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag
     3901 ctcccccgct ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc
     3961 ttgaggattt tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac
     4021 actatagtgc ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa
     4081 taagaccgta tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt
     4141 cttcctttct tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga
     4201 gtatcagaac aaaaataata acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt
     4261 tccctattcc ctcctctttt ttcaggtttt taaagcttt
//
LOCUS       DADQRX010000154         4282 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.162, whole genome shotgun sequence.
ACCESSION   DADQRX010000154 DADQRX010000000
VERSION     DADQRX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.162"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="J8V14_002670"
     CDS             complement(37..1257)
                     /locus_tag="J8V14_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBA0922737.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="J8V14_002671"
     CDS             complement(1277..1882)
                     /locus_tag="J8V14_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBA0922738.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="J8V14_002672"
     CDS             complement(1931..2863)
                     /locus_tag="J8V14_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBA0922739.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="J8V14_002673"
     CDS             complement(3284..4144)
                     /locus_tag="J8V14_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922740.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DADQRX010000155         4268 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.163, whole genome shotgun sequence.
ACCESSION   DADQRX010000155 DADQRX010000000
VERSION     DADQRX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.163"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..503
                     /locus_tag="J8V14_002674"
     CDS             75..503
                     /locus_tag="J8V14_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBA0922741.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="J8V14_002675"
     CDS             490..732
                     /locus_tag="J8V14_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0922742.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="J8V14_002676"
     CDS             complement(1032..1247)
                     /locus_tag="J8V14_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922743.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="J8V14_002677"
     CDS             1354..1668
                     /locus_tag="J8V14_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0922744.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="J8V14_002678"
     CDS             1772..2950
                     /locus_tag="J8V14_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0922745.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="J8V14_002679"
     CDS             3353..3646
                     /locus_tag="J8V14_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBA0922746.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1459 a          643 c          823 g         1343 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
     4261 aagcaata
//
LOCUS       DADQRX010000156         4250 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.164, whole genome shotgun sequence.
ACCESSION   DADQRX010000156 DADQRX010000000
VERSION     DADQRX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.164"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            832..1755
                     /locus_tag="J8V14_002680"
     CDS             832..1755
                     /locus_tag="J8V14_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBA0922747.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1771..2310
                     /locus_tag="J8V14_002681"
     CDS             1771..2310
                     /locus_tag="J8V14_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBA0922748.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3569..4003
                     /locus_tag="J8V14_002682"
     CDS             3569..4003
                     /locus_tag="J8V14_002682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922749.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1611 a          631 c          698 g         1310 t
ORIGIN      
        1 ttatgagcaa attaatgcaa aatttggcta gcaaaaaagc taaaaaatat atgctaataa
       61 tcgagcgaaa taaacacttt ttcaagtgag cgaatccgcc atgacaaagc tgaagcagtt
      121 tatttcagct ttgtcatggc gtgtgccgac ttttttaaaa gtctaggcac acgagcaaat
      181 atctttatca agctctaatt tgcgttttaa gacgtttgaa acgcctaagt atataaatat
      241 ataagaatag gtttaagagt gcttaaatcg aattttagag ggcatttaga caattttagg
      301 gttgttaggg cttgccctga ccgcctgtaa ggcgttttaa gaacaagata agggatttat
      361 actaggactg tcttaaaatg tcttaaacga acgctcagag cttctcaaag ggttccttat
      421 gaacggcttt tagcaaagga gccggaggcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttagcgaacc gtagggagca aggtttggaa ttggcagttt tgataaattc
      541 gcgaacctgt ttagagaaaa aaggagtgat tttttaggcg ggaaaattag cctcccctct
      601 ttttatatct ctttttatat ctctttttat atctctttta tgtcggctgt gtccttactc
      661 tcccaagcaa ttcagagttt tttcaagtag gttttctacg cttttcaagt aggttttcta
      721 cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tccgacatta ttgagtattt
      781 ttataaagta tgttactttt atttcatact ttataaaagt aggagaagct tatggcaaat
      841 gaaattgtaa aatatgaaaa tagattaaat catattcctc tgagaaaatt taatagtcgt
      901 gaaatgaact tatttttttc aattgcttca cgtgtacgtg aaaaaggtgt agatgagatt
      961 attttttcat ttcacgattt gaaaaaacta agcggttata cagaacgtgg ggaacaattt
     1021 ataaaagatc ttagtagcac gtatgacaaa ttattaagta ttaatgcttg gaatgatgat
     1081 ggaaatactt taactaaatt tgttgctttc acaaaatata gcattatacg agataaacaa
     1141 cttgttataa tttcagttaa tccagaattt aaaggacttt ttaatcaatt aaagaactgg
     1201 acaagattta atctagagca atttactaat ttggatagca catattcaaa aactatgttt
     1261 cgattattaa aacaatatcg aactcaaggc tggactgaat tttccaaaga aaaatttttt
     1321 gatttactag atattcctaa aagttattgg aatagtccgt caaatattga caaatttatc
     1381 ttaaggccaa ttaaagaaga actcactccc ttatttaaag gattaactat tcgtaaaaaa
     1441 tatggtaaag gtcgaggtaa acctgttatc ggttatcgtt ttacttggaa agctgaaatt
     1501 aaccatgctg acgatttttc caaagggaaa caagaagatt tacgtataaa attatttaat
     1561 attgatcaca atggagaatt aacacgagaa gaaaaatggc gcgctaaaga tcgaatacta
     1621 aatcttccct taggcactca tgaagctgat tttaaacaac aacaacaaac tgaagaaaaa
     1681 caggcagaaa aacgagaact ttcaaacaaa ctcaaacaag atttactagg aaatttacaa
     1741 aacttattcg attaagctta aggagtattt atggcagata aaacaatcaa agaacttgcg
     1801 gaagaattag gagttagtaa aacagcaatt aacaaaaaag tttctgacaa aaatcgaaaa
     1861 ctttggtttg caaaaaacgg aaacaagttt gtgattaacg aaaccggtca aaaagctata
     1921 aaatcaatgt ttttagtcga taacgcaaac ttaaatcgaa aactagttgg cgaaaaatca
     1981 aaaaccaaaa accataaaaa caccaaccaa gtttccgaat taaaactcct ttctttccta
     2041 gaaaaacagt taaatcgaaa agatatacag ctacaagaaa aagataatca aataaaacaa
     2101 atgcaaaaat tactcgacca gcaacaacaa ctcactctac aagcgaacca acaaattgag
     2161 aacctacaag aacagctcca tttaacttat acagaagaaa ctccaacaac cgaatcaact
     2221 gccttttctg aaaaaaaagc agacaataca caaccactta ttgaaaaaaa gtggtggcaa
     2281 atttggaaat ctaatagaac caatctctaa aattttctag aagttagttc ggatagtaga
     2341 ggaaaaagtt gtactacctg atggggagat cactcaaatt tttatgagaa agaaaaaata
     2401 gtttacaact gtaaacaatc aatgtttata gttgtaaact atcaaactat atggtgactt
     2461 attgcttaaa ataggttgaa atctcactca atttttggtg gaaaaacaaa ataaattcta
     2521 atcgattttg ttgaaaaagt cataaaaaaa caaatgtgat tttaacaaaa tttcaaaaaa
     2581 atcgtttgaa atttcgttta ttttttttga gaattttgat catcgtaggg tcattgtaga
     2641 atcattatga gatcatcgtg ggatcatcga ataaagtatg ggaaaggctt gcggtgcttg
     2701 gtattaaggg cattctccgc ctcccaggtt cttgaccatt ttctcttata agtgggattt
     2761 ttaccacgtt gagagaaaat ggtcaagaac ctgggaggcg gagaaaacat accaagcacc
     2821 acaagcaaaa aaagttagag taacttccaa aaaagaaaaa ttttttagaa caacttttga
     2881 aaatttaatt ttgatttata tccgttcaat ttaaacattt gataaactga aggctagtat
     2941 ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct atgttaaaaa
     3001 aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc cccatgctat
     3061 tatccgtaat tggaaaaaac aattccaaca gagaacttaa attgaattca aattattaca
     3121 acagtcctaa cgaagaattg tttgtaaaag aaacggataa ataataaaaa agactgctct
     3181 tccgagcagc cagttaaaaa ataatgagtg tttattcttg aaaaagcaac gcgcttataa
     3241 agcgctacca aaaatttatc atattttatg aaaaataaca atccagtaaa aaatgactgg
     3301 taaaaattca caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct
     3361 ttttacctag accaattaaa aatcacaaag tgagcccctg tatcggttga tacaagggct
     3421 acttggcata ctgtacatta tgtctactaa cctttaaaat aacacgtttt ataaaataaa
     3481 ccctcttgac aatatttaat tttctgatta ttctaaatat atacagaaag cgctttccta
     3541 ggattttata tctaaggagg aagtaacaat gaaaaataaa attattaaaa ttattacttg
     3601 tctatttttg ttcggtatga ttgttggagc aagtactccg ttaaatctgt ttccttttag
     3661 tgctagaggt ataaaagtgg aagctgcaag ttcacgatat aatcataatc atcgtggatt
     3721 tacttgctat aaaatgaact attatgttac taataaaatg tgtagagatt tgaaaaaaaa
     3781 ttataataag ctaaagactc ctgctcaaat tgcatctttt attcctattg gtggagttgg
     3841 aacatgggtt attacaaatg cttttagtag tgctgttgat gcaatgaatg tctttgtaag
     3901 agctgcaaat caaggaaaag gtgtacaatt aacatataat gctcacttta gcaatacaac
     3961 atcatatcaa tacaatgatt acgctcgtta tgtaataaaa taatcagaaa gtgaaactat
     4021 ctcatgaata aaaagatttt agctgtaatt atttcaataa tagttttaat cactatgttc
     4081 ttcatattta ggctaatttt caatataagc ttacaaaaaa gtattcttta tctaattcct
     4141 atagcattta tcttagcaat ttttagaagt atttacggaa ataaaaactc taaatagcta
     4201 aaaataaatc atactaaatt ttacaaaaag tggctagcat tttctagcca
//
LOCUS       DADQRX010000157         4238 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.165, whole genome shotgun sequence.
ACCESSION   DADQRX010000157 DADQRX010000000
VERSION     DADQRX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.165"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            38..475
                     /locus_tag="J8V14_002683"
     CDS             38..475
                     /locus_tag="J8V14_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922750.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            570..1454
                     /locus_tag="J8V14_002684"
     CDS             570..1454
                     /locus_tag="J8V14_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBA0922751.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1676..>4238
                     /locus_tag="J8V14_002685"
     CDS             1676..>4238
                     /locus_tag="J8V14_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922752.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1385 a          712 c          959 g         1182 t
ORIGIN      
        1 gaaaacgata ttaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct
       61 tttatttcta gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg
      121 aagtacaatg gatcatttgt atataaaata tattcatcaa ttttccgttt tggtattagg
      181 cacactaatt atagcgggga ttgttgcatt tattattcaa ggtcgtgaca tattaacagt
      241 acatcaagag tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa
      301 caaaaagtgg aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc
      361 taagattttt actttattag gtatacaatg cctatcattt tcttttattt cctatatcat
      421 tgttttaatt ttcctcagtt tcatagtatg ggtattttat cttgtcaaaa aataaatgaa
      481 tgtttcatga acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta
      541 aagaaatcaa tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca
      601 gttcaggcgg tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg
      661 tgtttgatga agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc
      721 gcgaagcggc tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca
      781 acgaaggaat cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat
      841 tgggtacggt gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga
      901 tcaatcattt ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact
      961 attttatgaa tgtcgtcgca attggctcta tccctgaagc aatcaatgat gtagatgcgg
     1021 agaagaagac gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt
     1081 ccacacaatc ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca
     1141 gtatcttgct gataggacta actaactcag taggcggttt tgaaacatta cttccaaatg
     1201 caaaagtgga tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata
     1261 cgcttaaaac attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct
     1321 atcttacttg tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg
     1381 ttgatggaga tgaaggggat aaattacctg tgacaatcaa agtgttaccc tcacatttac
     1441 aggtttattg ttaaaatgga ggttgtgacc gaagagttgt caggaggaca tcggttgttc
     1501 ggaataaggg actgtgacaa gacttttgtc acagcctttt tttgtacaaa aaagaaattt
     1561 tcacctaaca gaaaaatttg ccataaaaat acatatttta aatatgaaag tacaattaca
     1621 aataaattaa aacgttaaaa taaaattgtc aaaacgtcag agaggatggt ggagaatgaa
     1681 tcaatatgta aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac
     1741 atccgttttt gccgatgtag agaaaacaag cgaatcaacg caatcagaga atcaaagttt
     1801 tgacaagact actttttcag aagaatcaat tacttctgaa actacagaat cccaaagtga
     1861 ggcgcctact tctaatacaa ggcaagaaga aaataagaca agtgagacct cagaagtagc
     1921 tgaacaaacg tctgatagca gtgaagaggt acaggttaga gttgtttcag cggatgggta
     1981 cagcgaagct gccacaacag aagaactgac gcagcttctc gcagacgaat cagtagctaa
     2041 gattcgttta actcagcctt tgactttaga cagggaattg gagatcaagc gagatatcgt
     2101 tatcgatttt ggcggacttg ctcataattt cggcacacat cacatctata tcaatgagac
     2161 acctcagtcg atcgagtttc agaattttaa aggaacggca gctcatccag gtgggttgat
     2221 accaaatatc gatggaaatg ccattatcat tgcttatatg tccgattttt ggggaaacag
     2281 atatcatttt acgggagaaa taaaatttac cgggacactt gacttatatg atggttctaa
     2341 attaggcctg atttacgccc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtatt
     2401 ggatgtccaa ccagtgaaag aagggatgaa tgccgctccg ggtaaagctt atttttgtag
     2461 aagctatcag ctaaatgtaa tcaatggtag tcagctttat ggaccatatc taggaaaatt
     2521 ctatggtttt ctagattcag gtgatgagac gggcagtgga aaaacagcac caggactcca
     2581 tatcttgagt ggttcaaagg tctctcttga ctatgatcgg gcagatggcg tcagcgatga
     2641 aggagaggct gtcgatacgc tacccgggaa tgtcacattt aaagtcagtg gctcaggaag
     2701 tgaattttcg gtaaatacaa agatcaatat taccaacgac aacaatcgcg gaattatcca
     2761 aatgagaggc tcaggatcaa gagtcgctgt gtcgaatgat gggaaaatca caatcaatac
     2821 agcgactact ggcggatttc gcctgcaagg agattctagt caaatccatg tatcaagtgg
     2881 tggacaaatc tcggtcaaaa tggctggaga tggcgaccaa cctggaaaca atgggatgcg
     2941 ttttgtaggg aaaggacttt ctttgatcgt agaaggagcc ggatcagtca tcgatattga
     3001 taagcaaagt ggtcgttcta gcgcagttcg ttttgagaac ggtacgcaaa aactaacagt
     3061 aactaatgga ggctcattga aggtaagaaa tgcaggggat ggcaaatctt atgtgaacag
     3121 actcaatcaa ggaaaccaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc
     3181 agggtcagct gattttacgg ttactggaga aaaatcaaat atcgatatac gcgcagattc
     3241 cggagctacc gtagatacaa ctggagatat tgatttgaac tttatggctg gcgaaaaaac
     3301 ttatttagtc atggtcggga aaactgggga agataagact ggcatcatca gtggggatgt
     3361 gttgaatgta gacttagaat ctcctaccta tttcgatttc caaaacaaac ggatcggaag
     3421 cgagactaat ggcataggtg gctgggtatt tcaaggaaat ggcgattctt ctttgaagat
     3481 cgctcattca gaaattgctt tatggcgaaa ggcaggagta acgttcgata actttgaaaa
     3541 agatccggat tatttttctg atatgacaga cttatcgatc aatggaaccg acttaggtaa
     3601 ttttgtatca agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat
     3661 gaaaaattat aatcgaatca gtgccaataa ccagcttccg atgattgaat ccttaagagt
     3721 accaacgaat gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc
     3781 aaagccaaga gatgcttgga caggcgaagt agaagtgact ttacgtatta cttatgcaga
     3841 tacgacgaaa tcacctgtag aaattactgc tctcacacaa ggacaaacag atgaaaatga
     3901 agggacagga tataatccgt ggggagaagg cgaacaaggc ggtttgtttg aggttcaagt
     3961 acccaacgaa gaaagactaa caacggggga tcaagtgcaa atagttgctg ctaaaaaagt
     4021 aagaggcggc caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc
     4081 tcctgagcct gctaaattaa gtaccacatt gatcaatgca gcttctcgct caatcacagg
     4141 tacaggcgaa gcaggagctt tggtttcatt gaaaaaaaat caagctgttt tgtccgaaac
     4201 aactattgat gatacaggta aatttgttct ttctatcc
//
LOCUS       DADQRX010000158         4171 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.166, whole genome shotgun sequence.
ACCESSION   DADQRX010000158 DADQRX010000000
VERSION     DADQRX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.166"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..1376
                     /locus_tag="J8V14_002686"
     CDS             75..1376
                     /locus_tag="J8V14_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA0922753.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1403..1663
                     /locus_tag="J8V14_002687"
     CDS             1403..1663
                     /locus_tag="J8V14_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBA0922754.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2662..3897)
                     /locus_tag="J8V14_002688"
     CDS             complement(2662..3897)
                     /locus_tag="J8V14_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBA0922755.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1204 a          669 c          866 g         1432 t
ORIGIN      
        1 ataattagtt atgaacattc tttgcgttgt catgcaatga atggttcata ttttattgaa
       61 aaggagtggt tttagtggaa aagctgatga aatgggtcga agagaaactt gtcccaccaa
      121 tggcaaaaat tggtacacag cgtcatttat tagcaattcg taatggggtt gtttcaacat
      181 tgtcattgat tttgattggg acatttttta tggtctttat caatttaccg tttcctggat
      241 ggaatgaatt tattgcccct tattcggcaa cgattgtttt accctttagg atgacgatgg
      301 gattgatggc tatttatgca acttttgtta tgggttcgga tctagctaaa tcatatggat
      361 tagatagtgt gacaggtggt atattatctt taggaacgtt ctttatgtta caagttcctg
      421 taaacgtttt aactcctgaa gaggcacctc ttggctgggt cttacctatg agttctttgg
      481 gagcatctgg gatgtttgcg gggatcttat ctatgatttt tgcagtggaa gtatatcgtt
      541 tctttaaaaa tcgaaatatt actatcaaaa tgccagaaca agtgccacct gctgttgccc
      601 gtagttttga ggcattgatt ccaggtgcag tcattttaac aattacttgg ctgattcgaa
      661 gcgtcgcggg ctttgatgta aacgctgcct tattatcgtt attcgagcct ttaaccaata
      721 ttttagggaa taatttgctt ggtgttttac taccaatgtt tttgatccat ttgttgtggt
      781 catttggtat ccatggcatg agtattgtag gggctgttgt tcgtccaatg tggctgatca
      841 tgttggatga gaatgcaaag gcattggcag atggtacagc tgccacccaa ttaccctata
      901 tcgctcctga acagttttat caatggacgg taacaattgg tggtgcagga gcaacgatcg
      961 tggtatctgt tttgtttata ttcttctgta aatcaaaatt cttgaaagag gttggtcgtt
     1021 tttctattat tcctggtatc ttcaatatca atgagcccat aatttttgga gctcctatga
     1081 tgttgaatcc ttacatgttc atcccgttca acttagttcc tttagttttg actattatca
     1141 gttattttgc agtcaaatta gaaatggtca atagatttac tgttttgcca acatggacat
     1201 taccagctcc aatcggcggt tatctttcag caggaaatga ctggcgtgtt gttatcttga
     1261 tcatgatcaa cacattgata gcatttatta tctattatcc atttgtaaaa gcttatgata
     1321 aaaagatgct gtcagatgaa caaagtcgtg gagaaaacga agtccagact gtctaagttt
     1381 ggtttttaga aggtgggaaa caatgtattg gctgatttta ttttttgttt ttatcttttt
     1441 attaacggct agtcacctta tattgaatat gctagctaca tatcatattc aaatcaatcg
     1501 atggatttgg gcactggcat catttttgat cgtgattctt ccaaagatca ttgtaccgca
     1561 tatgaatgtt ttgttttcat gggggacata tgtactttgt ggaatttttg ctatcaattt
     1621 tatgatcgag cagcatagat ggtttgtcac atcgaagctt taaatgttct aaaagtggtt
     1681 gtaacagaag tggttagctt caagaaataa gaagaaattt acgaaaattg cttcttaaat
     1741 ttttgtgaaa tgttggctta tttttcgttt ggttgaactt attaccgcag atgcaagctt
     1801 ttgaccaccg tttattcgga atatgggact gtgacaaggc ttttgtcaca gccctttttt
     1861 acataaagat tattattgtt ttaactgttt tggcagaaag aaggaggaaa aaatggaaat
     1921 tttatcagca atggatttag aattagtaga tctactacca aagtgatcaa gtcaaatata
     1981 gatttgggag cttacaacca tatagtaggg gctgcagtat ggtgtatgaa tggtaatatg
     2041 tatgatgtga gtgtttattc aaatcatgaa acgtgtatag aaataattgt gctgggaata
     2101 gcaatcagtc atggtgaacg tgacttttag tgtatcgttg ctgacgacat agaacattct
     2161 gacatgatct actcaatatg tggggattgc tggcagttta tataggagta tgcatcggat
     2221 tgcgagatca ttgtctcaac agaagaggga ggttgattat caaagataaa gtggtaaatc
     2281 ctacatttga tttagtaatt gttgagggga ctagcggcac aaatctctac aaagcgatgc
     2341 ttctagatct atatgacaca ttttaagaaa atgtatttgc ctaattatta tggtctttct
     2401 tttgaagaaa cgttgagatg atccaaccag agaaaggtgt caaagaattt gactaagaat
     2461 aaatggcaga cgctacctat gctcatttgg atagcgtcag atcttttatt ttaaggatga
     2521 ttttgtgttt tttagacacc gtaatttaag taactctaac ttttcaatcc actatttact
     2581 ttttttgtat tttcactgtc tgggtctcga atagcgaaat tactgatgag tctctattaa
     2641 aaaagctttt tctacttgaa actatattgg gtggttgctc aggaaggttt cgatttcttg
     2701 aattgcttgt tgtggaccat ttattggaaa aacggcttgc agcgtgcgtg cctgctgacg
     2761 aatcttgggt gaagaaactt cgcgcagagc aatctgcagt tttctttcag tcactggttt
     2821 tgtgagggcg attgcgtttc cgtattttac acaatagtca atattacagc tttgctcaaa
     2881 ttgcatcccc acaccgataa acggtttgcc ggattcacag gcagtttgga cggtcccttc
     2941 cccgccgtgg ataaaagaaa aatcaatgaa ttcatgcaaa agatgactag acagatattc
     3001 tgttattaaa agatcttctg agtcagattg aatgtcatca agcagatagt caataggtgc
     3061 aataaccgct agatccggtt gtttttgtaa gaagaacaaa attttgcgta acatacgcgg
     3121 attgcctgaa ctacccattg cgaaatagat aatacgtttt ctttcttgtt gctttcgctg
     3181 tataaaagtt agcacttctt caggcaagga agtactgagc ttagcaaaaa ttggaccaac
     3241 gattcgataa ttttccggca gctgttcttc ttccaaaaat agtttaggcg ttgtgatgag
     3301 atttagatca gcatccatca tttgtaaact tgtttttggt aaaggaagca tttctttttg
     3361 tgcagcgtgt ttgaaggcat tggggatcca tcgggtattt actatacgct tttttgtcca
     3421 gtgccaaagg ggattgctta aagggccgaa gaaacgtaac tcgtggggca tcagcgcatg
     3481 ctctgattga aaaaaaggtt cactcaaagc atagggttta acatatacta aaggaatctt
     3541 tttattgcgg gccgataaaa aaattgtcac attcgaacct gtcacaatca gtcgaggctc
     3601 aaacttagca atcagactct gttcactagt gatacgagag gcaaccattt ctttggtaaa
     3661 agggttttta agtgtacgca actggtcaaa ttttatgatt tgttcttctt gttttttcgt
     3721 aaggatcggc gctagtaaat gatactcaaa gccactttct gtgatcaaag gagcaaaagt
     3781 tttagaaaag ccaaagaata cacagtaata acggtctttc atagctttgg caacctcaat
     3841 cattcgtgtt gtctcagcta aattgaatac tgcaggagca aaaagaattg tcttcatgaa
     3901 atcctcctta ttggaatgag tctatccctt cgtttcttac tagtatacta aaaaacacag
     3961 cgttcgagta gcctatgtat attttgaata ttaaactgtc tctcgctgtt ttcacaactt
     4021 ctaaatttaa attttgtaat caagttgaaa ctccaatttt caaaaaaaca cgcaacaatt
     4081 ttttgatcga tttatatgtt gtgccagata aatacgagaa gttgtgtcat taaaaataaa
     4141 catgagaaca taaaaaaata aaataattat a
//
LOCUS       DADQRX010000159         4163 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.167, whole genome shotgun sequence.
ACCESSION   DADQRX010000159 DADQRX010000000
VERSION     DADQRX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.167"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            352..1041
                     /locus_tag="J8V14_002689"
     CDS             352..1041
                     /locus_tag="J8V14_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA0922756.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="J8V14_002690"
     CDS             1028..2236
                     /locus_tag="J8V14_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0922757.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="J8V14_002691"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="J8V14_002691"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBA0922758.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1368 a          718 c          842 g         1235 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt att
//
LOCUS       DADQRX010000160         4089 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.168, whole genome shotgun sequence.
ACCESSION   DADQRX010000160 DADQRX010000000
VERSION     DADQRX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.168"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(93..635)
                     /locus_tag="J8V14_002692"
     CDS             complement(93..635)
                     /locus_tag="J8V14_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBA0922759.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="J8V14_002693"
     CDS             770..1159
                     /locus_tag="J8V14_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922760.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="J8V14_002694"
     CDS             1163..1927
                     /locus_tag="J8V14_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBA0922761.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="J8V14_002695"
     CDS             2056..3003
                     /locus_tag="J8V14_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA0922762.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="J8V14_002696"
     CDS             3133..3873
                     /locus_tag="J8V14_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922763.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1365 a          689 c          820 g         1215 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     3961 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     4021 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     4081 taaagaaga
//
LOCUS       DADQRX010000161         3923 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.169, whole genome shotgun sequence.
ACCESSION   DADQRX010000161 DADQRX010000000
VERSION     DADQRX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.169"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="J8V14_002697"
     CDS             complement(573..1130)
                     /locus_tag="J8V14_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922764.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="J8V14_002698"
     CDS             complement(1309..1920)
                     /locus_tag="J8V14_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922765.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="J8V14_002699"
     CDS             complement(2033..2290)
                     /locus_tag="J8V14_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922766.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="J8V14_002700"
     CDS             complement(2887..3165)
                     /locus_tag="J8V14_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922767.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="J8V14_002701"
     CDS             complement(3199..3450)
                     /locus_tag="J8V14_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922768.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DADQRX010000162         3909 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.170, whole genome shotgun sequence.
ACCESSION   DADQRX010000162 DADQRX010000000
VERSION     DADQRX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.170"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            658..1698
                     /locus_tag="J8V14_002702"
     CDS             658..1698
                     /locus_tag="J8V14_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HBA0922769.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2522..2854
                     /locus_tag="J8V14_002703"
     CDS             2522..2854
                     /locus_tag="J8V14_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBA0922770.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3473..3814
                     /locus_tag="J8V14_002704"
     CDS             3473..3814
                     /locus_tag="J8V14_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBA0922771.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1437 a          500 c          710 g         1262 t
ORIGIN      
        1 aattgcaatt tggtagtatt tttttattat cttattctta gaacggtagt acttaatata
       61 aatataggta gtatctaatt taagtattaa tggtagtatc taaattaaca ggtagtatgt
      121 aactacttta aagtaatttt tttcagatta acacagtagt atcttattaa ataagttaat
      181 tttaatggta gtacataatt attatatata tctgacattg gtagtactta ttgtattttt
      241 tagtagtata taatctattg ttttctagcc taaagccttg tcttataaga ataatataat
      301 atatactact gaaataaggt agtatatatt actagtaatg gtagtatgtt ttatatgcat
      361 aaggtagttc ttaatttatt ttttgttttg acactggtag tacttaatta tctttaattt
      421 taaaactaaa ttttattcat acgtttttct ggtagtattt aattataaat aatatttatt
      481 taaagtagtt cttaatgact ttaaaggtag tacataattc attttatttc tgattttagg
      541 tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt tttatttatc tttgctattt
      601 tcgaaaagag taggtaattt tacataaata aaacaacgtg aaagtagagg atctacaatg
      661 actgatttta aattttttaa ggcggataga gtttacaacg aattatttta tcaatttcca
      721 aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag attcaactaa gattgcctat
      781 atgcttttaa aagcaagatt agagatcgca atcagcaaac ggcaaatcga tgaagaaggt
      841 aatgtttatt ttacttatac gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa
      901 aaagcgatag caatcaaaaa agagttggaa tcctttggtt tattattaca aaagcagatg
      961 ggatttaaca aacagttagg gaaaaataat cctaatagac tatatctagc agaattaaaa
     1021 gtctcagaaa atgatatcta cttactcgaa aaatttgata gagagaatag ggaaaacgtt
     1081 gataaatcag agggtatgaa aatcataccc accctcgacg aaaaatcaga cgctgaatcc
     1141 cttggggctc aagagggtat gaaaatcata ccgtgccaaa acgttgataa atcagagggt
     1201 atgaaaatca taccagaact taataataat atattagaca ctaatagaca caatatagac
     1261 actgaaaaag accgcctaca agatcaattg ttgttagaca attttgagac aattatgaca
     1321 aacgacagca ttgctacgtt tgtccctgaa cgagtattaa atttgataaa aacattttct
     1381 tcaagttaca gtgaagctca aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct
     1441 gaaatagaaa gtgatatttc gatagttttt gaagaactcg atagttatta tgtcaatgca
     1501 gaacaagaat tatacacgac actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc
     1561 gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata
     1621 gctgctaaag tatcaagtga aaaacgtttg aattatgcaa gctccccaag cactattacg
     1681 aaagactggt tagagtgaaa ctttactgac aaagtgattg ttgcttcatg gagtaacatt
     1741 aaaattaaaa attttttatt attctaatta tattacgaat attacaaaat aatagctatt
     1801 ttaatagaat ttataagtta aaatattatg ataaaaatca caaacgtagc cttttacgtc
     1861 ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat gtttgcatac
     1921 tccttttttt tgagttataa taatacgcga agagagataa tttcattatt taaacaagct
     1981 aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta cattttttgt
     2041 ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca cttttggatg
     2101 ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg
     2161 taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg ttaacctttc
     2221 agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag tgataccact
     2281 ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat gcctaagcat
     2341 aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa attagaggga
     2401 acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc aattctctat
     2461 ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg tggtttattt
     2521 tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta aacggtgtac
     2581 cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg ttgtgcagtg
     2641 taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt acattgggaa
     2701 acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc caacaatcaa
     2761 acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg
     2821 taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata acttgtattt
     2881 tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt agagtcacta
     2941 gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga cggggtaggt
     3001 tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg
     3061 gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt tagaagttag
     3121 acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct agactttgac
     3181 gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt
     3241 gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga tcaatcgagc
     3301 atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt
     3361 atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa
     3421 aattaatata aagcatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa
     3481 ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga
     3541 ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc
     3601 attagacgag gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt
     3661 gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg
     3721 agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg tcagctttga
     3781 aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat
     3841 aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt
     3901 aaggaattt
//
LOCUS       DADQRX010000163         3863 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.171, whole genome shotgun sequence.
ACCESSION   DADQRX010000163 DADQRX010000000
VERSION     DADQRX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.171"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(245..466)
                     /locus_tag="J8V14_002705"
     CDS             complement(245..466)
                     /locus_tag="J8V14_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922772.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="J8V14_002706"
     CDS             complement(564..809)
                     /locus_tag="J8V14_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922773.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="J8V14_002707"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="J8V14_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="J8V14_002708"
     CDS             complement(2033..3034)
                     /locus_tag="J8V14_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBA0922774.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="J8V14_002709"
     CDS             complement(3193..3459)
                     /locus_tag="J8V14_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922775.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="J8V14_002710"
     CDS             complement(3449..3805)
                     /locus_tag="J8V14_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA0922776.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DADQRX010000164         3851 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.172, whole genome shotgun sequence.
ACCESSION   DADQRX010000164 DADQRX010000000
VERSION     DADQRX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.172"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..1157)
                     /locus_tag="J8V14_002711"
     CDS             complement(3..1157)
                     /locus_tag="J8V14_002711"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBA0922777.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="J8V14_002712"
     CDS             1342..3783
                     /locus_tag="J8V14_002712"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBA0922778.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1232 a          721 c          840 g         1058 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgcttt t
//
LOCUS       DADQRX010000165         3703 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.173, whole genome shotgun sequence.
ACCESSION   DADQRX010000165 DADQRX010000000
VERSION     DADQRX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.173"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..850
                     /locus_tag="J8V14_002713"
     CDS             <1..850
                     /locus_tag="J8V14_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA0922779.1"
                     /translation="HMHMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYDSVS
                     VKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMC
                     RFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQS
                     YFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDISSAV
                     RDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLY
                     QKGSKE"
     gene            1418..2479
                     /locus_tag="J8V14_002714"
     CDS             1418..2479
                     /locus_tag="J8V14_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA0922780.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            2797..3387
                     /locus_tag="J8V14_002715"
     CDS             2797..3387
                     /locus_tag="J8V14_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBA0922781.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT         1369 a          396 c          727 g         1211 t
ORIGIN      
        1 tcatatgcac atgtcgtaca gagggagttt tagtaggaaa tatgtgctga ataagtggtt
       61 tagattatta aatattcctg taatcatcca tcttcatgga tcggaattta aaaaatggta
      121 tgatagtgtt tctgtaaaaa aacaaaatca gattcgcttg ctgttaagag agtctgattc
      181 cacgattgta cttggagaaa aatggaagaa gataattaaa gaaattgagc caaattgtaa
      241 aactgttgtt gtagaaaatt ctattgtagt tccagaatat accataaagt ggaattcaaa
      301 aatgtgccga tttctgttta tgggagtttt gataaagcga aaaggtgtac acgatttggt
      361 agaagcagtt aaaaacattg cagaacaata tccagaaaaa aagtttattt ttgagattgc
      421 tggagcaggt gatgaagaaa tatcgctcaa agaattgata gaagaatata atttgcaatc
      481 gtattttgaa tttacaggtt gggttgtggg agaaaaaaag gaggagtgct atagaagatc
      541 aaatgtgatg attttaccgt cctataacga gggattgcct atatctattc tagaggcgat
      601 ttcttatgga atgcctgtaa ttgctacaga tgtaggagat atttcttcag cagtacgtga
      661 tggagaaaat ggatatttgt ttcaacctgg tgacgtatca gcgttgacat ctgcaatact
      721 aaaaatcaat gataaagata aatacgtaaa attttcacgt tgttcgaagg atttggcaat
      781 aacgaaattt aacgatggaa atttctttga tagaatttta aatctgtatc aaaaagggag
      841 caaggagtga tgacaaggaa aataaaaaat gtttcgatta tgtaatgaac ttattaagtg
      901 aataaaatag agagaaaaaa tattgaaata gacgatgtaa atttttatcc gtgtaagaat
      961 tcaaatgaat agtagtgttt tagtgcttgt aatagagcaa gagtacaagc acggtacatt
     1021 atataaaaac attaatgata tttctataaa taaatagatt ttgtattctg gatgatgaag
     1081 aggaagatat tatctgataa cttaccctaa actttgcagg gggtggttat aagttgtgta
     1141 tatgtggcag taatgaatga gtgaaataat catattaaga aataaaattg caatattgtg
     1201 taatacaaaa gtatattttc attattccaa gggaaagcat acttacgagg atcatattat
     1261 tgatttaata aataatttaa aggaaataat ttttctagaa gaagatagta tgtcattcac
     1321 atcatcaaga tcagagtcta tttttttcag agaatttaag aaaatcgttg atgccgtcat
     1381 tgactaattg agaaaacgtg agtgaaagga tagaaggatg aattcgagtg atctaattag
     1441 tgtaatcata cctgtatata atgttgaaaa gtatgtagga agttgtttgg aatcagtgtg
     1501 taatcaaaca tataagaacc tagatattat aattgtggat gatggatcta aggattcatc
     1561 taataaaata tgtgaagact atgcattaaa ggattcaagg attcgtatta ttcataaaaa
     1621 taatgctggc ttgggattgg caagaaatac aggacttgaa catgtaaaag gaaaatatgt
     1681 tgcttttatt gattctgatg attttgttgc agctaattat attgaagcaa tgttaaatgg
     1741 tataaaaagg agtaatgcag acacatgtta tgctggatat tatgaatatt atgatgataa
     1801 taatattatt tcaaagccag caagatatga tagtacgata tttacaaata acgctataat
     1861 tgaaaaagtg ttgcttggaa tgattggcac tgagccgagt tataaagtcg attttatatt
     1921 gccaatgtca gtttggcatg cgctgtattc atctgagata attcaacagc acaatatcaa
     1981 gtttccgtca gaaagggatt atatatctga agatatcata ttccatattt catatttgca
     2041 acattcaaaa ataacaagtt ttattaaaga ttgtatctat tattatagat gtaataataa
     2101 taattcattg acacataatt ttagagataa tgagttcgat tgtaatattg aattagttaa
     2161 aaaaattgaa ggcgatttaa gctatatatt agaaaatgag atatttgtaa acaaaacaag
     2221 gagacttttg ttgggacgct gtcgtacttg tattcaaaag gcagttagat atgctagttt
     2281 taataaagaa tttgatttaa actcctattt gtctaagata cttatgaacg atgagttgat
     2341 tgcctgtatt cactcatacc catataaaca aaatccaatt aaacaacgaa tatttaacta
     2401 ttttttacta aaccaatcca ttattggggt taaaacatta gcatatttaa gcggaatggg
     2461 tagaaatagg agattttaat cataaaaaac tgtactatag tgaatttttg gagatttata
     2521 ttccagttat atattaatga ttgtgatact tgtactattg taaaaattcg ttaggtttaa
     2581 ataactggaa atatcggaaa atgggcatta tacatcacgg acatcccttc ttttggaaaa
     2641 aatgttacga tttttataag taatattgta tccggtggaa tcaacgatga agataacata
     2701 tccatcgttg tagattcagt agcattgaat gatgctcttg ttgcaagaaa tccgaataaa
     2761 attgttaagt ataaattgaa ttaataggag atatgaatgg aaattagcag taaagaatat
     2821 ttaaaagcgg ttcttcgtat ggaaaagaat ctatatatag aatctaaagg acaacacaca
     2881 aaattactaa aaataatgaa atcacatccg gactacaacg cttggcggta tactcgtcga
     2941 atgagaatta catcttatta ttattcaaag agaaagaaaa atatcattta tgcgattatg
     3001 tatattgtgt cttgcagaag aatgaatagt ttgggaagaa aatgtggtat agagtgtggt
     3061 gaaaatgtat ttgatgaagg tgtaagattt taccatacac aaggaattgt tattaatggt
     3121 aacgccataa taggaaaaaa ttgttactta tatgggaata actgtattgg aaatgatgga
     3181 atcgatccta agtgtcctgt aattggaaat aatgtacgtg tatgcgtggg tgcaaagatt
     3241 attggtaatg taaaaatagc taacaatgta gttgttgcgg caggagctat tgttatcaaa
     3301 gattgtctag agaatggtgc aattcttgca ggtgtaccgg caaaaataat agggtatgtt
     3361 aatttaacaa aagtattttt agaataattt ttagcgaaag aaggtgaata acgatgcgta
     3421 tttcaataaa aagatggtca ggagcagatt ggacattgtt cacgttggca ttttttgcaa
     3481 tctccatagt tcttgtatat acttttaacg ccccaagagc aattttatat ttgggagata
     3541 taattaatat ctttgtgtta ttaaatgcac ttagctttag acgcagaaaa ggaatatatg
     3601 ctagcaaaaa tgagatgctg atagttttgt ttttactagt ttctatttgt agctcagtta
     3661 ttaatttaga aaatatagga ttgattatct gggggatacg aaa
//
LOCUS       DADQRX010000166         3696 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.174, whole genome shotgun sequence.
ACCESSION   DADQRX010000166 DADQRX010000000
VERSION     DADQRX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.174"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="J8V14_002716"
     CDS             363..731
                     /locus_tag="J8V14_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBA0922782.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="J8V14_002717"
     CDS             911..1852
                     /locus_tag="J8V14_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBA0922783.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="J8V14_002718"
     CDS             1921..2652
                     /locus_tag="J8V14_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBA0922784.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..>3696
                     /locus_tag="J8V14_002719"
     CDS             2796..>3696
                     /locus_tag="J8V14_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBA0922785.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADE"
BASE COUNT         1238 a          618 c          819 g         1021 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagc
//
LOCUS       DADQRX010000167         3653 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.175, whole genome shotgun sequence.
ACCESSION   DADQRX010000167 DADQRX010000000
VERSION     DADQRX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.175"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..276
                     /locus_tag="J8V14_002720"
     CDS             <1..276
                     /locus_tag="J8V14_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBA0922786.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="J8V14_002721"
     CDS             419..640
                     /locus_tag="J8V14_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922787.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="J8V14_002722"
     CDS             656..1498
                     /locus_tag="J8V14_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922788.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="J8V14_002723"
     CDS             1561..3363
                     /locus_tag="J8V14_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBA0922789.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3653)
                     /locus_tag="J8V14_002724"
     CDS             complement(3422..>3653)
                     /locus_tag="J8V14_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBA0922790.1"
                     /translation="KNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSVKGL
                     ESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1304 a          517 c          741 g         1091 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt tta
//
LOCUS       DADQRX010000168         3614 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.176, whole genome shotgun sequence.
ACCESSION   DADQRX010000168 DADQRX010000000
VERSION     DADQRX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.176"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1357)
                     /locus_tag="J8V14_002725"
     CDS             complement(1016..1357)
                     /locus_tag="J8V14_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBA0922791.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="J8V14_002726"
     CDS             complement(1357..1587)
                     /locus_tag="J8V14_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA0922792.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="J8V14_002727"
     CDS             1874..2212
                     /locus_tag="J8V14_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922793.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2656)
                     /locus_tag="J8V14_002728"
     CDS             complement(2417..2656)
                     /locus_tag="J8V14_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBA0922794.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2632..2979)
                     /locus_tag="J8V14_002729"
     CDS             complement(2632..2979)
                     /locus_tag="J8V14_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HBA0922795.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1156 a          675 c          557 g         1226 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcga
//
LOCUS       DADQRX010000169         3602 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.177, whole genome shotgun sequence.
ACCESSION   DADQRX010000169 DADQRX010000000
VERSION     DADQRX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.177"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..186
                     /locus_tag="J8V14_002730"
     CDS             <1..186
                     /locus_tag="J8V14_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922796.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="J8V14_002731"
     CDS             329..553
                     /locus_tag="J8V14_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922797.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="J8V14_002732"
     CDS             568..1437
                     /locus_tag="J8V14_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBA0922798.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="J8V14_002733"
     CDS             1418..2152
                     /locus_tag="J8V14_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0922799.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="J8V14_002734"
     CDS             2185..3093
                     /locus_tag="J8V14_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBA0922800.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="J8V14_002735"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="J8V14_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DADQRX010000170         3546 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.178, whole genome shotgun sequence.
ACCESSION   DADQRX010000170 DADQRX010000000
VERSION     DADQRX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.178"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="J8V14_002736"
     CDS             complement(<1..2232)
                     /locus_tag="J8V14_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922801.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="J8V14_002737"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="J8V14_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1008 a          780 c          551 g         1207 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaaca
//
LOCUS       DADQRX010000171         3540 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.179, whole genome shotgun sequence.
ACCESSION   DADQRX010000171 DADQRX010000000
VERSION     DADQRX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.179"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(266..970)
                     /locus_tag="J8V14_002738"
     CDS             complement(266..970)
                     /locus_tag="J8V14_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922802.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1157..1402)
                     /locus_tag="J8V14_002739"
     CDS             complement(1157..1402)
                     /locus_tag="J8V14_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922803.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1419..2270)
                     /locus_tag="J8V14_002740"
     CDS             complement(1419..2270)
                     /locus_tag="J8V14_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA0922804.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2436..3440
                     /locus_tag="J8V14_002741"
     CDS             2436..3440
                     /locus_tag="J8V14_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBA0922805.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          603 c          687 g         1152 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
     1141 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
     1201 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
     1261 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
     1321 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
     1381 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
     1441 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
     1501 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
     1561 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
     1621 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
     1681 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
     1741 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
     1801 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
     1861 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
     1921 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
     1981 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
     2041 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
     2101 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
     2161 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
     2221 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
     2281 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
     2341 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
     2401 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
     2461 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
     2521 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
     2581 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
     2641 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
     2701 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
     2761 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
     2821 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
     2881 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
     2941 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
     3001 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
     3061 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
     3121 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
     3181 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
     3241 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
     3301 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
     3361 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
     3421 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
     3481 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
//
LOCUS       DADQRX010000172         3528 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.180, whole genome shotgun sequence.
ACCESSION   DADQRX010000172 DADQRX010000000
VERSION     DADQRX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.180"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            89..562
                     /locus_tag="J8V14_002742"
     CDS             89..562
                     /locus_tag="J8V14_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HBA0922806.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            636..1076
                     /locus_tag="J8V14_002743"
     CDS             636..1076
                     /locus_tag="J8V14_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBA0922807.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            1089..1298
                     /gene="copZ"
                     /locus_tag="J8V14_002744"
     CDS             1089..1298
                     /gene="copZ"
                     /locus_tag="J8V14_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBA0922808.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            1298..3484
                     /locus_tag="J8V14_002745"
     CDS             1298..3484
                     /locus_tag="J8V14_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBA0922809.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
BASE COUNT         1213 a          603 c          813 g          899 t
ORIGIN      
        1 tttttttgcg gcgttataag tggtatgatc gaaccaaatt ccaaagagaa gtgagggaga
       61 agaatgaaga tcatcgtgat tggacaaagt gggtcgggaa aatcgtccct cgcaaggaag
      121 atcaaagaaa tcaccggctt tccgcattca ggcaaaaaag tggtttttgc aagaacagca
      181 atcgtttatg gtatctaatc cgtcatggat cgttgaagga aattatactt ctacgttaga
      241 ggaaaggatt aaagaagcag ataagatcat ttggcttaaa gtgccgcgtt atctcgccat
      301 ctaccgagta gtttggcgtt ctctaaaaag aaagataaac aaaaaatctc ggccagatat
      361 gcctgaatca ttttcggaga aactaaacag gaaatactta gaatttttat cttttatctg
      421 gcattttgaa ggaaacaatg aaccaaaaat acagcagctg atccaagtag ctaatgcaag
      481 aaataaactg atcatcctta aaacaagacg agagaaacaa gaatttttaa ctaaattgaa
      541 aatagagagc aaaaataatt gacagttttt gtttacaagt gtaatctgta ttcataagtt
      601 aatgattaca tttgtaaacg aaaggtgtga aataaatgga aaaagtcaaa acgccagtaa
      661 agattagtga ttcagagtgg gagatcatgc gtgttatttg gacacttgga cagacgacag
      721 cccaagaaat cacgcagatc ttagctgatt cgatggattg gaaaccagca acgatcaaaa
      781 ctttgctggg tcgtttggta aaaaaagaag tgatctggac agaacaagaa ggaaaaaaat
      841 tcatttatca tccagctgtc tcggagatgg aaacagttcg cagtgcgacg gaaaatcttt
      901 tttcccatat ctgtgcaaaa aggattggag agacgattgc ggatctagta gaagaagcca
      961 ctttgactca agaagatatc aatgagattt tgaaacaact ggaacaaaag caaccagtag
     1021 atacgatcga atgtaactgt atcccaggtc aatgtgaatg taaacaacat caataggagg
     1081 aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag ctgcaatcat tgtgcagcaa
     1141 gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa agtgaaagta aatttgaaaa
     1201 aagcaaacgg tacggtcaag tttgatgaaa caaaagttca atccgaaaag atttgtcaag
     1261 caatcaatgg attgggttat aaagcagagg tgatttgatg gcaacagcag aaaccaaaat
     1321 ggatacgttc gtcattacag gtatgacttg tgcaaattgt tctgctagga tcgaaaaaga
     1381 gctgaacgga caacctggtg tggtcaatgc cacagtcaac ttggccactg aaaaagcaag
     1441 tgtcaaatat gaagggacga ccactgaaaa attaatccaa agcgtagaaa acatcggcta
     1501 tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt gcagaagaaa agcaagcgta
     1561 tttgaaaaaa atgctattcg atcttatatt gagtacggtt ttgactttgc cgttgatgtt
     1621 gtcaatgatt gctatgatgt taggaagcca tgcggcgatt gttcactttt ttcattttcc
     1681 aatcgtccag ttagtgctgt ctgctcctgt ccaattttat gtcggtgctc gtttttataa
     1741 aggtgcgtac catgcaatca agtcgaaagc accaaatatg gatgtattgg tagcaattgg
     1801 aacatcggct gcctttgcat tgagcattta caacggattt ttccgtggac acccccaaga
     1861 tctttatttt gaaagcagca gtatgatcat cacgctgatt ttgctcggca aatatttgga
     1921 acatacagca aaaacgaaaa caggtaatgc aatcaaacaa ttgatgtctc ttcaaacaaa
     1981 aaccgcccaa gtgataagaa acgggaaaga agaaacgtta gcgattgaag aagtagtcgt
     2041 tggtgatcaa ctagtcatcc gtcctggtga gcagattcca gcagatggtc gaattatttc
     2101 aggaagcagt gcgatagatg aaagtatgct tactggtgaa aatcttcctg tagaaaagaa
     2161 tccggatgac acgctttttg gcgggacaat caatacgaat ggtctgctac acatggaagt
     2221 cacacaagtc ggcaagcaga cagtattagc gcaaatcatt caaatggtgg aagatgctca
     2281 agggagcaaa gccccaatcc aaaaaatcgc agatcggatc tcagggattt ttgttccgat
     2341 cgtattagtc atcgcgttta tcacgctcat agctacaggt ttgatcactg gtgattggca
     2401 actcgcattg atccatagtg tctctgtatt agtcattgcg tgtccatgtg cattaggttt
     2461 agcgactcca acagctatta tggtaggaac gggtgttggt gcacgaaatg gaattttaat
     2521 taaaggcgga gaagcattag aagcagctgc tcacttagac agcattgttt tggataaaac
     2581 tgggacgatt acagaaggaa agcccaaagt tacagatcta gtcggttcta aagaagtcct
     2641 atctattttt tatactttag aacaagcttc tgaacatcca ctaggtaaag caatcgtgga
     2701 atacggaaaa ctgcaagaag cagcaacata cgatatgatt gattttacag ctcacccagg
     2761 tgctggaatc agcggtacga ttaacggagt acgctatttt gcgggtacac gtaaacgatt
     2821 aattgaattg aacctatcat ttgatgaata ccaagaacac gcgttagaat tagaacagca
     2881 gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg atcgggttga ttgccgtggc
     2941 tgatcaaatc aaactagaag ccaaacaagc aatcaagcag ctccagaata aaggattaga
     3001 tgtttttatg ctgacaggag acaacaaact ggcagccgaa acgattggta aacaagttgg
     3061 tattgatcca aagcatatct ttgctgaagt acttccagag gataaagcag cctacgtaga
     3121 aaaacttcag aaagatggca aaaaagtcgg tatggcaggc gatggaatca atgatgcacc
     3181 ggctttagca ttagctgatg ttggtatggc aatgggaagt ggaacggata tcgcgatgga
     3241 aacagccgat gtgacgctga tgagtagcag tttggcatcc attgcacaaa caatcgagtt
     3301 gtctagggtg acattgagaa aaatcaaaca aaatcttttc tgggcttttg tctataatac
     3361 gattggtatt ccttttgccg cactaggctt tttgaatcca atcattgcag gaggagcgat
     3421 ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc ttaaatcggc acatgaaaaa
     3481 gtaagagaag ggatggagta aaaatgaatg ggaacgatat ggagaaaa
//
LOCUS       DADQRX010000173         3469 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.181, whole genome shotgun sequence.
ACCESSION   DADQRX010000173 DADQRX010000000
VERSION     DADQRX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.181"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            293..505
                     /locus_tag="J8V14_002746"
     CDS             293..505
                     /locus_tag="J8V14_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922810.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(783..2582)
                     /locus_tag="J8V14_002747"
     CDS             complement(783..2582)
                     /locus_tag="J8V14_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA0922811.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2706..3302)
                     /locus_tag="J8V14_002748"
     CDS             complement(2706..3302)
                     /locus_tag="J8V14_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA0922812.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1274 a          625 c          603 g          967 t
ORIGIN      
        1 gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata tgctcacctc
       61 tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg aatataaaca
      121 aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa aagcagaaag
      181 atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa ggtaatttgt
      241 tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg aaatggtaaa
      301 agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg aactaatcgg
      361 ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt atgaccaacg
      421 agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct gtgatattag
      481 cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga actcccttat
      541 aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag ttcagggaaa
      601 atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat tgttcgtgcg
      661 atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa aacgcatata
      721 cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt aacgattttt
      781 gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat ttcttcatct
      841 gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc atccacacca
      901 taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata tacttctttt
      961 ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc cattgtcaga
     1021 aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta gcccacataa
     1081 aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact ttgcaaaata
     1141 tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac aaggtcatcc
     1201 gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt atgtgctttt
     1261 tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt aactgtaaga
     1321 tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt taagtgacga
     1381 agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac atccatctct
     1441 acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt attttgcgcc
     1501 ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg aacagctgaa
     1561 gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc aaaaagagtc
     1621 aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat aaaccactta
     1681 gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata aaaaatccct
     1741 acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat agcaaacaca
     1801 gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc caaaataaca
     1861 acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat aaattggccc
     1921 gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc tgctcgaaaa
     1981 ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc aatatagcca
     2041 aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc aaaaataaaa
     2101 tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa acttaatccg
     2161 ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat gggtctcact
     2221 ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg ttctttcttt
     2281 ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa cagtaaaatc
     2341 gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa aattgaagga
     2401 atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg ggtcgtactg
     2461 ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac atcacggaaa
     2521 taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt caaataattc
     2581 atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa agggtgcagt
     2641 gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat tttacaaaat
     2701 aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc catactatat
     2761 aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt cactgacatg
     2821 tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag aaaaatagct
     2881 agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata tttgttatat
     2941 gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc tagcagaaaa
     3001 atgataatcg aaccaatgca gattccaata tagttataga taaatccagc ataagggcca
     3061 aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat aactacttgc
     3121 aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc tactaatcct
     3181 cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt cagcgcgatt
     3241 gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc agcaatattc
     3301 attattcatc aactccttag aggttctttg ttaactatac tttactagaa aagcaactaa
     3361 ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc ataagtataa
     3421 aagattgatc tcaaaaaaac atccaactaa agacttattt aataacttg
//
LOCUS       DADQRX010000174         3435 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.182, whole genome shotgun sequence.
ACCESSION   DADQRX010000174 DADQRX010000000
VERSION     DADQRX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.182"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            105..905
                     /locus_tag="J8V14_002749"
     CDS             105..905
                     /locus_tag="J8V14_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBA0922813.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            921..1799
                     /locus_tag="J8V14_002750"
     CDS             921..1799
                     /locus_tag="J8V14_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBA0922814.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1812..2792
                     /locus_tag="J8V14_002751"
     CDS             1812..2792
                     /locus_tag="J8V14_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBA0922815.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT         1182 a          561 c          716 g          976 t
ORIGIN      
        1 ctaattatta tatgtgtgtt attgaactaa ctcttgacag ataatcaaaa agaatttata
       61 gttgttaccg tagtagctac taaaaataaa gtgaggatta atcaatggaa acgattattc
      121 aaatcatgaa aaaatatgat ttttcagaga tggagacaag agtgtatacc acactattag
      181 aaaaaggtaa tcttacagga tatgaagtaa gtaaaatctc aggtgtgcca cgctcgaaag
      241 tatataatat tttggaaaaa ctattaaaaa agaatttgat tgtcgttaat aaatcagaac
      301 ctaaacttta tcatgccatc tcggcaaatg agtttttaga gaaattagaa aaaagtgtta
      361 aaaatgatct ttcttttctt accaagaatc tagggatgat caaagaaaaa gatgaagaag
      421 aaatgctgtg gaaagttgat ggtatcgagt atgttctgga taaagtagag catttggtga
      481 aaaacgcaaa agaaagtttg ctcattcaag tttggcatga gaatttcacg gattcactac
      541 taaaggcatt gcagcaagct gaaaaaagag ttgataagtt tgtactgatt ttattcagtt
      601 ctacccatga atatgatctt cccttagaaa agtattatat tcatggtttt gaaacagaca
      661 aacttgctga ctttggagca agatggatca acatcgtcgc cgatgagcaa gaagtcgtgt
      721 ttggtaccat taacgaagaa ctgcaatcaa ccgatgtcac ttggacaaaa aaccacgcca
      781 tggtcaatct agcaaaagaa tatgtcaaac atgatgcata tacactaaaa gtgattgcag
      841 aatctcctga agaacttaaa gcaaaatatg gtgagggctt tgagggaatc agaaagattt
      901 actagtagga ggaatttttt atgattcgga atattttgct gacactaatt gtcgcaatca
      961 atctatattt tattatttac tttctaagag accttttcaa aaacaaacaa aatttcaaag
     1021 aagaaccagg ggatatgcgt ctgttgccct tcacgtcatt tatcatcttc tttctttcaa
     1081 catttggcgt ttccgacttt gctatcggaa ccgtccttta tccaaagttg aaatgggtca
     1141 gcatgaaaaa actacccgga acgttgaata cccaatgtgt agtaccggta gccgtcatgg
     1201 cattgtctta catcactgcc atcaacgtag ggatcaagac actagctgtt tgtattatct
     1261 gccaaattat cggtgcatat ttaggaccaa gatttgtcgt gaagttacca gagaaaacga
     1321 tcaaactgtt tgtcggcata ggattggtca ttgctgcatt gttgattttt gcaggacaaa
     1381 tgaactggat tccatcaaac ggaacagcca gtgaattgta tggcggaaaa ttaatcttgg
     1441 caggattttt actctttgtc tatggtgcat tgaacaatat cggcattggt tcttatgcgt
     1501 taacgatggt aactgtctac ttgctaggct tgaatccagt tgcagccttt cctattatga
     1561 tgggggcttg taccttctca gtaccgatcg gcagtgttca attcattaaa tttaatgaat
     1621 acagcagaaa aatcacactt tttacttcaa ccttcggtgt attgggggta ttggtcgctg
     1681 tattcctagt caaatcttta gatacctata tcctgaaatg gatcgtgatt ttggtcttac
     1741 tatacagtgc atatacgatg ttgggcagtc aattaaaaaa agttacagca gctaattaag
     1801 gagcgattca aatgttattt cttaaaaaag aagatattat caaaagtttt tcaatgagag
     1861 aagccatcga tgcggacaaa aaagcactgt cattgtattc agcaggaaag gcaagtgtgc
     1921 ctcttcgcac caatatcgat gtgccaaaaa gcaacggaca aagcttatat atgcctgctt
     1981 atgtagaagg tggcgaaggc gcgctaggtg tcaaaatcgt ttccgtgtat cctgaaaaca
     2041 tcaagaaaaa cttaccaagt gtaccagcaa cgatgatcgt attggatcca gaaacgggta
     2101 tggtatccgc ttgtttggat ggtacttatt tgacacaatt aagaacaggc gctgttcaag
     2161 gagcagcaac agaacttctt gcaagagaag atgctaaaat tggcgcgctg attggaaccg
     2221 gcggacaagc acagtcacaa ttagaagcaa tgttgaccgt tcgtaaatta gaagaagttc
     2281 ggatttttga tattgatttt gagcgtgcca gccaatttgc tgaaaaaatg atgcaacaat
     2341 tttctgtaac gatgcgccca acaaaaacca gtcaagaatg cgttgaagga gcagacatca
     2401 ttacaagtgt gaccacttca aaacgtgcga cattctctgc tgagtgggtc aaaaaaggtg
     2461 cgcacatcaa tggagtcggt gcttacacac cggaaatgtg tgaaattcca cgagaaataa
     2521 tcaaagccgc agatgttgtt atttttgata ccatggacgg tgttctaaaa gaagctgggg
     2581 acttcatttc accgctgcaa gatgggtata ttcaaagaga cagctatcat ggggaactag
     2641 gccaactgat caatgaagaa ttgatcggtc ggacaagtgg agaacaaatc acgatcttca
     2701 aaacagttgg ttcggctgta ttagatgtgg tagttgcgac agaaatcgtt aagaaagcta
     2761 aagaaaataa tttgggaaaa ttgattttct aattaaaaag gctgaaatta aaacctttat
     2821 tattgcgttt aaaaaaagaa aacaaagaag tttgtaatca aatggaaact gaccccataa
     2881 tttaaataaa ataaaaaaca tctcttgttg aattctagta atgaagcgac cctaaaactt
     2941 agaccaaaaa atctaacttt tgggtggagt tgtcaagaaa aaccgggcac ttagttaaaa
     3001 aataagttgg acgggttata ctaaaattga caccaaatta agagaaagcg aagatctgga
     3061 ttatgccaag acgttcattt gatgaaatat ttaaaattac cgctgtaaaa ctggtaacgg
     3121 aagaagggtt ttctgttaaa gaagttagtc tatagctaga agctcgctct aatagcgtgt
     3181 attgatgagt tcaagaagtt gaaaaatatg gataacgtac ttttctcggt agaggaagtc
     3241 atgaaatcca ttcgcagaaa tgaaattaca cgcttagaaa aagagaataa acggttaaaa
     3301 gaataggtag aactcctaaa aattaccgaa gtcttcctga aagaaaaggc tcttcgtcaa
     3361 attgtgttgg cgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc
     3421 gccgcatatt tttta
//
LOCUS       DADQRX010000175         3324 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.183, whole genome shotgun sequence.
ACCESSION   DADQRX010000175 DADQRX010000000
VERSION     DADQRX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.183"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(94..166)
                     /locus_tag="J8V14_002752"
     tRNA            complement(94..166)
                     /locus_tag="J8V14_002752"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Gln,seq:ctg)
     gene            complement(212..640)
                     /locus_tag="J8V14_002753"
     CDS             complement(212..640)
                     /locus_tag="J8V14_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922816.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(700..1458)
                     /locus_tag="J8V14_002754"
     CDS             complement(700..1458)
                     /locus_tag="J8V14_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HBA0922817.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1753..1950
                     /locus_tag="J8V14_002755"
     CDS             1753..1950
                     /locus_tag="J8V14_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922818.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2056..2538
                     /locus_tag="J8V14_002756"
     CDS             2056..2538
                     /locus_tag="J8V14_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922819.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2596..3192)
                     /locus_tag="J8V14_002757"
     CDS             complement(2596..3192)
                     /locus_tag="J8V14_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBA0922820.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1009 a          555 c          641 g         1119 t
ORIGIN      
        1 aactaaatac aagtagtaaa aataatacaa taatccccct gcaaaaagat tgttctttta
       61 cagggggttc attttttcag caagcacatc aaattgggat ggcagggttc gaacctacaa
      121 ttgcttgatt cagagtcaag tgccttacca gtttggctac atcccattta ttcaacaaaa
      181 taatcatagc actttcctaa aaaaacgcaa gttatttttc ttgatcggat agttgttgct
      241 ttactcgtaa aaggcggcgt actagtttct tttctaatcc agcaggaagg gcaatcagtt
      301 ctgctactga caagacggtg tccaataact gatattcttt gacaggttca ttcataatat
      361 gatagatgtt tgctaaacgt tcaatatatt caaagcgagt atactctgtt cgagcaatta
      421 gaaatttcaa taaatcggtg gctttctcgt actcttgatc tgcgtaataa acaagagatt
      481 gacgatacaa tttgatttcc agttcattga gggtagcagg tctgcgacgc tcgttccact
      541 cattaataga gactttacct tgaacatttc ccttgcagtc acggacaaaa ataatcggaa
      601 tttcatttgg atccattttt ttatgtgtga atttttccat tatctacgct cccttcaaag
      661 gtttggtatc ttgagtataa gggatttttg tttgaattac tagcttttct ccaaagttag
      721 catctgtcca cccattgcag tgaaaatcgc cgaaaaatcg ttgatcggta ctgctcgatt
      781 ttttacacca tagggatcgt tgacataaat attttctttg tcatatccag tgactacaca
      841 agcatgaaca agaggagtga ctcttacttc tccattattt gtggtccaag tcataaaatc
      901 agtttcatct gggacttcaa aattgatagt cacagaagtc cagacaggat ttcctttaga
      961 tagtgctttt ttgatatcat cgagttgagc atcggttttt ggaaaagtgt gataatccgt
     1021 ttggacgatt tgctgagcaa gggaggcaac tgggggaaca tagacgacca tagcccagtc
     1081 tccgccactg atatcaccaa caaatccttc attaggatcg ccatgagtcc catctgcatt
     1141 gaaaacaggg acgtaatcca attgctcagc taactggttt ttagttgttt cataaccata
     1201 aaaagataaa agcatagaaa gcgaagtgac ttcacagcca ttttctaaag aaggagaatc
     1261 aaactggctc tctaatggaa catctagtaa aacttctgct ttctcttttg tctttttcgt
     1321 tttttgtgtt gaactttttt gtgcagtgtt cattgtatgt tctggagaat ggatatgtag
     1381 gtaataactt gtaccgctta ataaaagagc agctgtgccc cctagaaaca atagtttttt
     1441 gaacgttttt ttcttcattt gaaatctccc ttttcagctg tttatctttc ctattataaa
     1501 tgtattttta ttggaaatgg aagtattata ttttaatatg taggaaataa ttagttttta
     1561 aggaatatgt tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt
     1621 gaaaacgaaa acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat
     1681 caatgagtta aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt
     1741 aggaggattt tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca
     1801 attggtttta caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg
     1861 gtcaaagttg cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg
     1921 gttgttgcaa aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca
     1981 agtccaattt ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa
     2041 gcgaggcgag ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg
     2101 atgtttttat ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga
     2161 tgggtctact tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt
     2221 catcctacag cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt
     2281 ttgttggaca gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa
     2341 ggtgttcctt tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc
     2401 gtaaaaatga attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca
     2461 ttagaagaat tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt
     2521 atgattgatc cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt
     2581 ttatttcaaa taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt
     2641 ttcctcagcc ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga
     2701 agggatattc cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt
     2761 tgcgcccaat cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga
     2821 ttgttctgac caatctggga agttgtctgc atataattca aattgttctt gtagaccttt
     2881 taccacgtca gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa
     2941 tttgttttgt gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt
     3001 gatttcccaa agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc
     3061 attgaaagca gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt
     3121 agaaagtgaa acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt
     3181 aaaagttgtc ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta
     3241 cgaaatgaag tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag
     3301 cgaagttcaa aaaacaattt taat
//
LOCUS       DADQRX010000176         3200 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.185, whole genome shotgun sequence.
ACCESSION   DADQRX010000176 DADQRX010000000
VERSION     DADQRX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.185"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="J8V14_002758"
     CDS             301..585
                     /locus_tag="J8V14_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922821.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="J8V14_002759"
     CDS             588..1004
                     /locus_tag="J8V14_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922822.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="J8V14_002760"
     CDS             complement(1141..1860)
                     /locus_tag="J8V14_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBA0922823.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="J8V14_002761"
     CDS             2513..2608
                     /locus_tag="J8V14_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBA0922824.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..3156)
                     /locus_tag="J8V14_002762"
     CDS             complement(2911..3156)
                     /locus_tag="J8V14_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922825.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DADQRX010000177         3160 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.186, whole genome shotgun sequence.
ACCESSION   DADQRX010000177 DADQRX010000000
VERSION     DADQRX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.186"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..302
                     /locus_tag="J8V14_002763"
     CDS             39..302
                     /locus_tag="J8V14_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBA0922826.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            296..646
                     /locus_tag="J8V14_002764"
     CDS             296..646
                     /locus_tag="J8V14_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBA0922827.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(670..1284)
                     /locus_tag="J8V14_002765"
     CDS             complement(670..1284)
                     /locus_tag="J8V14_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922828.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1598..2020
                     /locus_tag="J8V14_002766"
     CDS             1598..2020
                     /locus_tag="J8V14_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922829.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2030..2233
                     /locus_tag="J8V14_002767"
     CDS             2030..2233
                     /locus_tag="J8V14_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922830.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2444..3028
                     /locus_tag="J8V14_002768"
     CDS             2444..3028
                     /locus_tag="J8V14_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922831.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1093 a          467 c          577 g         1023 t
ORIGIN      
        1 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
       61 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
      121 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
      181 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
      241 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
      301 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
      361 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
      421 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
      481 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
      541 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
      601 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
      661 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
      721 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
      781 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
      841 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
      901 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
      961 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
     1021 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
     1081 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
     1141 ttcttgaaaa acaagtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
     1201 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
     1261 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
     1321 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
     1381 taccttatat agacaaaaac taatgatgga ggaaatagta tgtgtcactt ttctttttaa
     1441 atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat ttaaacgatt atgacattaa
     1501 aaatatattt tatgaatttt ctagtatata acttgattta tctgttaaga tttattaaga
     1561 aaacgttttc ttatttttga tttcgaggtg atagacaatg aaaaataagt ttattttatc
     1621 tttaggtatt ttaactattg gaattagtgt aactggaact acaattccta catttgctga
     1681 tagtacaaat gaaccaacta ctgtagtaga taataaccaa gaattaactc ctttagatag
     1741 gaacggaata gccattggta acccaactaa cgacttaact gttactgaaa attatttaat
     1801 agtttctcca ttacttgctc gagcagcttc taaaacgata cgagtgacag gaacgatgat
     1861 ttttgataaa gttggttggc cttctagacc tcctcaatca cggaattgga aagaaaaaag
     1921 aaatggttat tggtactatg gaaaattgta tttacaaagt tataaatcag ctggaaacaa
     1981 ttggattgcg acttattcag gaaccttaac tagaaagtag ggatttttta tgccagaatt
     2041 tgtgaacaca ccaaattttt atcaagatag cgatattcta agtaaaatta atttaccgaa
     2101 agaagaatca gctttacaag ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc
     2161 aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca
     2221 aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg acattctttt tttctaattg
     2281 aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta tttttcaaaa
     2341 aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg
     2401 gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
     2461 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
     2521 ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga
     2581 aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt
     2641 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag
     2701 ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat
     2761 gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc
     2821 agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc
     2881 tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat
     2941 tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga
     3001 gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca
     3061 tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat
     3121 tctcaaatgt tgtaaggatt ttattcattt gttaatatta
//
LOCUS       DADQRX010000178         3129 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.187, whole genome shotgun sequence.
ACCESSION   DADQRX010000178 DADQRX010000000
VERSION     DADQRX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.187"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..2376)
                     /locus_tag="J8V14_002769"
                     /pseudo
     CDS             complement(14..2376)
                     /locus_tag="J8V14_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            2824..2997
                     /locus_tag="J8V14_002770"
     CDS             2824..2997
                     /locus_tag="J8V14_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA0922832.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
BASE COUNT          844 a          656 c          567 g         1062 t
ORIGIN      
        1 cgattttatc attttattgg tcaagatttt tccatttcca atccaatact tctggcagat
       61 caataccatt ttcacgaata tagttttgat gttcttgtaa tttctgatcc atttttttcg
      121 caaaatcttc tgcttgactt cctttgattt ttaaagctgc gtcttttgcc aaatgataac
      181 gatcaagttc atttactaca cgcatgtcaa atggtgtcgt gatatctcca ttttctttgt
      241 agccatgaat cattaaatga tggttatgac gatcaaagaa aatcgtgcgg accaatgttt
      301 cataaccgtg gaaagcaaat aagatcggtt tgtcttttgt aaaatattga tcaaattctt
      361 catcgctcaa cccacgcgga tcttgacttg gatgacgtaa tttcaataaa tctacgacat
      421 tgatgaaccg aatttttagt tttgggaatt cttccagcag caaagttaca gctgctaatg
      481 cttctaagtt agattccgtt cctgcagcag caatgaccaa ttcaggttcc tcctcttttg
      541 ctgtcgaagc ccaatcaatg attttcaagc cttcattcac taagacggct gcttcttctg
      601 ctgagtaaaa ttgagcacgc ggatgtttgg aagagatgat caaattgatt ttttctgacg
      661 agcggaatgc tttatccatg actgcgagca atgtatttgc atccgcaggc agatattcac
      721 ggatgaactc cgccttcttt tctgccaaat gtgtcaatat tcctggatct tggtggctat
      781 atccattgtg atcttgctgg aagactgttg agcttgcaat gagatttagc gaaggataat
      841 catttcgcca agaaaggtca tgtgatttcc gcatccattt gaagtgctga gtcaacatag
      901 agtcaaccac tcgcaagaat gattcgtaac ttgcaaaaaa ccatgccgtc cagtcagaac
      961 gtacccttct aagaaccctt catcttggtg ttccgataat tgactgtcga tgacgcgacc
     1021 agatggcgaa agccattcat cattttctgg ttcgattttt tccatccatt ggcgatttgt
     1081 cgttttgaaa acttgattca atcggtttga ttttgtttca tctggtccaa atatacggaa
     1141 attttctgga tttttctcaa tgatctcgcg aacgaattta ccaagttcgg tcatatcttc
     1201 tgctttaacg gagcctggtt tttcaaattg caaagtatat tttttccaat ctggcataat
     1261 aagcggtctt ggatcgattc caccattggt gataggattt tttgccattc tttggtcacc
     1321 agtaggcgcg atttctctga tttcttctaa aatacgtcct tgtgcatcaa ataatttttc
     1381 tggttcatag gatttcagcc aacggagtaa agcatctgca tgatccatat gttcttgatc
     1441 tacaggaatc ggtacttggt gtgctcggaa ggttccttca attggttccc cgtcccattc
     1501 ttttggaccg gtccatcctt ttggtgtgcg tacgatcaat actggccatt ttggcatcac
     1561 gacatctgtt gctgtcttca ggcgagcttc tttttggatg gatttaattt tctcgattgc
     1621 ttgatccatt ttttcagcca taacaggatt taatttttct gaatcatttc cttcaataaa
     1681 gaaaggttcc cagccaagtc cgttaaagta atttgctaat tctccatctg atttacgtgc
     1741 caagatcgtt gggttggcta tttttgcacc atttaaatga aggatcggta aaacagctcc
     1801 atctatcact ggattgataa agacatttga gaaccagctt ccagctaaag gaccggtttc
     1861 tgcttcacca tcaccaatca cgacagcaga gattacttca ggattgtcta atacagctcc
     1921 gactgcatgt gaaagagagt atccaagctc tccgccttca tggatagaac ctggtgtttc
     1981 tggtgctgca tgagaagcaa tgcctccagg aaatgaaaat cgtttgaata atttttgcat
     2041 tcctgtctca tcttccgtta cttctggata gatctctgtg taacttccat ctaaataagc
     2101 attcgatacc atgacttgtc cgccgtgtcc tggcccttca atataaaaca tgttcaaatc
     2161 atatttattg atgactcggt tcaaatggac ataaataaaa ttttggccag gaatcgttcc
     2221 ccaatgaccg attggatgtt ttttgacatc ttcgggtttc agtgtacgtc tcagtaaggg
     2281 gttatcttta agataaagtt gcccaacaga aagataatta gcagcacgcc accaagccgt
     2341 cattttatca aagtattctt tactagaata atccatacat aacactcctt ttcattatta
     2401 ctcttattct agcgcagata ctcccaattg aaacctaata agcacttctg ttcatttgtt
     2461 tggattttat atctagccaa atgaacaggt ataaaaaacc acgaaatttt tcctttcgct
     2521 gacgtttttt acattgatcc aaaaaacttg tcgttatcac aatggctgtt ccgcagttca
     2581 tttcgctcct catcatgcat aatttgctga atgacgctgg gccagttaac acattgctgg
     2641 aaaactgggg ctggatcgag caattccttt cctgattgat ccgttttatg taaaagtctc
     2701 tgttatcttt gtcaatatgt gaattggtat cccagctact atgctaattt ctacagggat
     2761 tatccaaaat ttggatcgtg accaaatcga agcggcgtaa tcggaaatat caataacttc
     2821 aacgtgattt ttcttttaac tgtcaacgga ccgacaaact caaactttta tggtgcaggt
     2881 tccactgatc ttctagttac ttggctatac ctgactgtca ctaaaataga ttacaatcta
     2941 gcttccgtta tcggtatctt gatctttatc tatatgcaaa aatttatata ctactagaaa
     3001 aaagaaagtc gaatttttta cttttctttt tctctttttt ggttagctga cacgccgcaa
     3061 gactaaagtc atggaactct taggtaatac gtcttaatga aaacaaaaaa atcccgcaac
     3121 ccctgattt
//
LOCUS       DADQRX010000179         3111 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.188, whole genome shotgun sequence.
ACCESSION   DADQRX010000179 DADQRX010000000
VERSION     DADQRX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.188"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1350
                     /locus_tag="J8V14_002771"
     CDS             <1..1350
                     /locus_tag="J8V14_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBA0922833.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="J8V14_002772"
     CDS             1484..2827
                     /locus_tag="J8V14_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA0922834.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..>3111
                     /locus_tag="J8V14_002773"
     CDS             2939..>3111
                     /locus_tag="J8V14_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0922835.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1232 a          430 c          589 g          860 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt t
//
LOCUS       DADQRX010000180         3089 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.189, whole genome shotgun sequence.
ACCESSION   DADQRX010000180 DADQRX010000000
VERSION     DADQRX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.189"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..814
                     /gene="fabI"
                     /locus_tag="J8V14_002774"
     CDS             59..814
                     /gene="fabI"
                     /locus_tag="J8V14_002774"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBA0922836.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(924..1937)
                     /locus_tag="J8V14_002775"
     CDS             complement(924..1937)
                     /locus_tag="J8V14_002775"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBA0922837.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2102..3022
                     /locus_tag="J8V14_002776"
     CDS             2102..3022
                     /locus_tag="J8V14_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBA0922838.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          898 a          623 c          589 g          979 t
ORIGIN      
        1 attattctct atttttttac gactatcaaa tttttatcag aaacaaagga gagtcattat
       61 gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc
      121 ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata cttaccaaaa
      181 tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg
      241 tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg
      301 ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat tgtccggaaa
      361 tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca gctactcttt
      421 aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga tcgttagcat
      481 gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta ttgcgaaagc
      541 ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag gaattcgggt
      601 caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta aagactatca
      661 aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga cgatcgaaga
      721 agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca tcggtgatat
      781 tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct ttaaagtaaa
      841 acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc tttttacgtt
      901 tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc tggtgcttct
      961 atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac tggtcctact
     1021 tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc gattggagaa
     1081 ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc atcaatgaaa
     1141 atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa caaggaatat
     1201 ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc gtcttttgta
     1261 attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc tggatcaaga
     1321 taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa actcgctgcg
     1381 taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc gtaatcagat
     1441 acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg cgtacagaag
     1501 acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac cccaatcagt
     1561 aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct
     1621 tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg catcagcaat
     1681 cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag attgaccttg
     1741 ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata atgaagcgct
     1801 ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc ttcccattgt
     1861 ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa acctagattg
     1921 ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt tatcattatc
     1981 catattattt gtcattttat tttcagcttt tttccttcat tatagttatt tttgctattt
     2041 ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa aagaggattt
     2101 tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga cattgacaga
     2161 tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg attgggacgc
     2221 cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg tccttttttg
     2281 gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt attctaaaaa
     2341 agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata ctatttttca
     2401 ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg tgccgatcgg
     2461 atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt
     2521 catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg atgttgggga
     2581 ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg gtatccgtac
     2641 ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa acatcaccac
     2701 gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga ttgttcctga
     2761 agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat tagctgaaaa
     2821 ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc ttggaaacag
     2881 caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg ccttaaaaga
     2941 agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga ttccgaacag
     3001 tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac taaaaagagc
     3061 gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DADQRX010000181         3041 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.190, whole genome shotgun sequence.
ACCESSION   DADQRX010000181 DADQRX010000000
VERSION     DADQRX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.190"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..964)
                     /gene="murB"
                     /locus_tag="J8V14_002777"
     CDS             complement(29..964)
                     /gene="murB"
                     /locus_tag="J8V14_002777"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBA0922839.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1037..1957)
                     /locus_tag="J8V14_002778"
     CDS             complement(1037..1957)
                     /locus_tag="J8V14_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA0922840.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2070..2822
                     /gene="xth"
                     /locus_tag="J8V14_002779"
     CDS             2070..2822
                     /gene="xth"
                     /locus_tag="J8V14_002779"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBA0922841.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1047 t
ORIGIN      
        1 ttttacttat tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata
       61 ttgcttgttt ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt
      121 tgattacttc ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga
      181 tattgacgat gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt
      241 gcaatcctgc ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac
      301 ttccgcatga agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca
      361 ttcttgcttt gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga
      421 tagcacgtaa ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca
      481 aagttttcaa tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct
      541 ctccgccata cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc
      601 aagcaaattc aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg
      661 aggcacctgc ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca
      721 gcatgatgac tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta
      781 ataccatcca aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt
      841 gttttttcgg gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca
      901 tcaatggttc atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat
      961 tcactgagga ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca
     1021 atcttgccag tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata
     1081 attcatgact tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac
     1141 ggatctgaga aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg
     1201 tccagtcgcc aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca
     1261 gatcgtttac ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc
     1321 cacttgctaa atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg
     1381 catattccaa aaattccttt gtttctctta tctggtattt tgttctcaaa atgggttcat
     1441 ctaataaata cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg
     1501 tcgtttcttt tgccgctatt cgattttttt gcaggtggat caaccgctta tctgggatac
     1561 gtttcaattc ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt
     1621 ggttcttatc agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt
     1681 tgtcaatata aacatggata ccattttcta gacatttttt ggctaattca taatgtactt
     1741 ttgttgctgc gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt
     1801 catatagatg ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg
     1861 ttgcaaaata aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt
     1921 ctgcgatatt ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta
     1981 gttactttca tcttacacca ttttttacta aaatgaggta caatagtatc gcaaaggtaa
     2041 ggaaattaat tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc
     2101 ttcgtgccat cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt
     2161 tctgtctgca ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt
     2221 atcaatattg gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa
     2281 aaccagcatt gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc
     2341 tgattacttt ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa
     2401 gcgaattaaa acgtttagat taccgattag aatgggaaga agccttttat aattatttgg
     2461 aaaatctaaa aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa
     2521 agattgattt gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa
     2581 gagcggctct ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc
     2641 caacccagga aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg
     2701 ctggttggcg gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg
     2761 caaagatcca tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat
     2821 aaagaggttg tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc
     2881 tttccacatt ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta
     2941 tcgagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
     3001 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga t
//
LOCUS       DADQRX010000182         3022 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.191, whole genome shotgun sequence.
ACCESSION   DADQRX010000182 DADQRX010000000
VERSION     DADQRX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.191"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..175
                     /locus_tag="J8V14_002780"
     CDS             <1..175
                     /locus_tag="J8V14_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922842.1"
                     /translation="DSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLM
                     LIKKPKEILSKIS"
     gene            185..841
                     /locus_tag="J8V14_002781"
     CDS             185..841
                     /locus_tag="J8V14_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA0922843.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            841..2217
                     /locus_tag="J8V14_002782"
     CDS             841..2217
                     /locus_tag="J8V14_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922844.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2503..>3022)
                     /locus_tag="J8V14_002783"
     CDS             complement(2503..>3022)
                     /locus_tag="J8V14_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBA0922845.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1145 a          399 c          532 g          946 t
ORIGIN      
        1 ggattcaatg atttccggtg gaagtttaat aaataatagt agttttatgt caaatataac
       61 aacacttgga caaagttatt taatattaat aagtattatt gttttatcag ttgtatttgc
      121 ttcttcatta atgttaatca agaagcctaa agaaatctta tcaaaaataa gttaggagaa
      181 aataatggat atattagaaa taaagaatgt agcatatagt tatgcaaatt ctaaagaaaa
      241 agttttgtca ggagtaaatc aaaaatttga acttgggaag ttttatgcga tagtaggaaa
      301 gtcaggaaca ggaaaatcta cacttctttc cttacttgct ggacttgata aacctcaaac
      361 aggaaaaata ttgtttaaga atgaagatat acaaaataaa ggatatagta atcatagaaa
      421 aaataatata tctttagtat ttcaaaacta taatttaata gattatttat caccaattga
      481 aaatattaga ttagtaaata aatcagcaga tgaaagtatc ttgtttgaac taggtttaga
      541 taaaaaacaa ataaaaagaa atgttatgaa attatctggt ggacagcaac aaagagtagc
      601 tattgctaga gcattggtat cagatgctcc aataatacta gctgatgagc ctactggtaa
      661 tctagacagt gttactgctg gagaaataat taatatatta aagacattag ctaaagatag
      721 aaataaatgt gtaatagttg taacacatag taaggaagta gcagattctg cggatatcat
      781 tttagaacta agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg aggttgaata
      841 atgataaaaa atgcatttgc ttatgtaact agaaaaagtt taaaatcatt aattatcata
      901 ttagttattt tatctatgtc aactttaagt ctcattagtt tatctattaa agatgctacg
      961 gacagagctt caaaagaaac atttgctaat ataacaaata gtttttctat ggagataaat
     1021 agacaagtaa atccaggaac acctagaggt gggggaaatg taaaaggtga agatattaaa
     1081 aagatatctc aaacagatag tatagactcc tatgtaaaaa gaataaacag tgttgcagat
     1141 ttagttgatt atgatattat tgaaactcaa gagactcttg cgaatcaatc acctgaaaga
     1201 gcaaagaatt ttaaaagaac agttatgtta actggggtta acgactcaac aaaagaaaca
     1261 aaatttgtat cagaagcata taaattagta gaaggaaagc atttagaaaa tgaagataaa
     1321 aataaaatct taatgcataa agatttagct gaaaaaaata accttaaagt tggagataag
     1381 ataaaaataa aatctaattt atttgatgct gataatgaga aaggtgcaga tgaaacagta
     1441 gaagtagaaa ttaaaggtct attcgatggg cataatagcg gtgtagtaag tgcagcacaa
     1501 gaactatacg aaaatacatt aatcactgat attcatacag ctgctaaagt ttatggtaat
     1561 accgaagata cagctgtata ccaagatgca acattctttg taaaaggtga taagaatctt
     1621 gatagtgtaa taaaagatct tgggaaatta gatataaatt ggagagaata taatttgatt
     1681 aaaagttcat caaattaccc tgcattacaa caatctatat caggtattta ctcaatttca
     1741 aataaattat tttttggctc attaatattt gcaggagttg tagtttcatt attattattc
     1801 ttatggatga atgctagaaa gaaagaaata gcagtattac tatcattagg tatatcaaaa
     1861 ttagaaattt ttggtcaatt cctaattgag atggtattta tatcaatccc cgcattcgtt
     1921 ggttcctatt tcttagctca atatacagct gacaagcttg ggaataatat attgaataag
     1981 gttactggtg atatagctaa acaaatagct aaacaatctg catctagcca attaggtggt
     2041 ggggcagaag ctgaaggatt taacaagaca ttatcaagtt tagatatcaa tgtattacct
     2101 aaattcatga tttatgtagt tatatttatg agtttagtac ttcttgtatc attgattatt
     2161 tcttcattca atatattaag aaggaatcca aaagaattat taattgacaa taattaaaat
     2221 tttcggtgct ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca
     2281 atcctaggcg ggtggttgct tcacggtttt tatttttcta aaataagatc aagaggtgat
     2341 tacacgatgg ctagaaagaa aagaaatcct gatgctgaga agttagctga atccattttg
     2401 aatacttatc aacccgaatc tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt
     2461 cccttatttg aaaaaatgct tcaaggagaa ttgaataacc atttagggta tgacgcccat
     2521 tctaaagagc caaagaaaca tgacaatcga cgaaacggtt atggcaataa gacgcttaaa
     2581 acgagctttg gtaaagtaaa cattgatgtt cccagagacc gggaggcttc ttttgaacct
     2641 gagttgattc ctaatagaaa acgagatgtc tccgacatcg cagactgtag acaaaagctg
     2701 ttccaaactc gtaactcgag cttggaacag ctttttatct acttcgtttc gaatttttga
     2761 acaaaaaaat aaaggctcct tgcctttcca tagccaggtg gctaatttct ttaaattcat
     2821 tgcagcaaac actagcatcg tatgcgtcgc gactttttcc aagcctcgat acttggtcca
     2881 acgcatccca tgcttttctt ttgcatccgc aaatattcgt tcaatcgttt gtttccgttt
     2941 tttgtaaatt gatttattca gatctgttag gcgaagatgt tccacttcat ccatcagatc
     3001 tccccaaaca tgtctcgtga tg
//
LOCUS       DADQRX010000183         2932 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.192, whole genome shotgun sequence.
ACCESSION   DADQRX010000183 DADQRX010000000
VERSION     DADQRX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.192"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..189
                     /locus_tag="J8V14_002784"
     CDS             <1..189
                     /locus_tag="J8V14_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922846.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="J8V14_002785"
     CDS             complement(275..1369)
                     /locus_tag="J8V14_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBA0922847.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="J8V14_002786"
     CDS             1558..2319
                     /locus_tag="J8V14_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA0922848.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="J8V14_002787"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="J8V14_002787"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBA0922849.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          938 a          581 c          496 g          917 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tc
//
LOCUS       DADQRX010000184         2904 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.193, whole genome shotgun sequence.
ACCESSION   DADQRX010000184 DADQRX010000000
VERSION     DADQRX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.193"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            196..582
                     /locus_tag="J8V14_002788"
     CDS             196..582
                     /locus_tag="J8V14_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBA0922850.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            680..871
                     /locus_tag="J8V14_002789"
     CDS             680..871
                     /locus_tag="J8V14_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0922851.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1385..1717
                     /locus_tag="J8V14_002790"
     CDS             1385..1717
                     /locus_tag="J8V14_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922852.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1627..1830
                     /locus_tag="J8V14_002791"
     CDS             1627..1830
                     /locus_tag="J8V14_002791"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA0922853.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2014..2169
                     /locus_tag="J8V14_002792"
     CDS             2014..2169
                     /locus_tag="J8V14_002792"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBA0922854.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2445..>2904
                     /locus_tag="J8V14_002793"
     CDS             2445..>2904
                     /locus_tag="J8V14_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922855.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPK"
BASE COUNT         1030 a          510 c          487 g          877 t
ORIGIN      
        1 atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt
       61 gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc
      121 agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg
      181 gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc
      241 tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa
      301 aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt
      361 aaacaaccga ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa
      421 atccttcctt tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttaat
      481 aaaactgata aagaaattgg aaaggttctg ggtattacaa gacagggtgc gacaaattta
      541 aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa
      601 ttttgtatgc ttctttgctc gtcctcctcc ttccccaaaa aataaataga ttataagttc
      661 atgggaagaa ggtaaattta tgaatacttt aaaagaagat ttcattttag caaagaatgg
      721 aaatgaagaa gcagttgtgg ccattctcaa aagatttagc gctttggtcc ataagcaatc
      781 ctggagaact ggaaaatatg atcaagattg ttatcaagaa tgtatgcttg caatatatct
      841 agctatctcg aaattcgaaa ttaaagaata actcaactaa gggagtctgt gacataagta
      901 aaaagatagt ttcaaatttc gaacaataaa tttgaagcca tcttctttat aattataaag
      961 tatcgatttt acagcgttct ataatgattc atagaatcta aaacgaatga ttttaattat
     1021 taacgttttt taattcgttt ttagcttaaa aataactttt gtcacagtct cactcctaaa
     1081 attactttat ttacgtacga agtgggcagg aggtcgaatc cagaactggg ccatggtact
     1141 tcaacgagga cttctcctct agaattgaaa agcatgcgat ttaccttccg taacgacggt
     1201 agatttattc aactatgtgt ccaatcaaaa tttgtctgat ttcaaaaata gtcaagagaa
     1261 ttttggttgt taaaaactct tatctatcaa cttccacagt ttagttgaca aaccctgata
     1321 ctaagttggg ctatgataca cttttcttga ctatcagtta cgttaccgga aggaatgaac
     1381 tctattgaaa aaaacatatg atcgtaaatt taaactaaag atttcacagg atatccttga
     1441 gaaaaaagta actaccaaga aaattgctga agaatacaat atctctcgtc caacaatttc
     1501 ccgatgggtt tcagaatatc gtcgatacgg gaaaaatgct tttgctggac aaggaaaaag
     1561 attacctgat aaagcagatt tctacattct tgaacaggaa aataaacgac taaaagagga
     1621 agacgagtgt tctacgaaca caaagatcga tacggatctc cagggatcac tcaaaaactt
     1681 tacgatgaag gaatcgaaat caataaacgt gttgtagctg ttcttatgcg tgaaatgaat
     1741 ttatgcacaa gaggatttca tcgacgcaaa tcttctcatg gtagagaaaa aacaatcaaa
     1801 gaaatggtaa aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg
     1861 gtgacggata ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt
     1921 gacttagcaa ctcgtattct acgttactat atggtggatt cacacatgaa aaaagagatt
     1981 gtcgtccgtc cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga
     2041 ggtagccagc atcggtcttt tatttatcaa gagctgttga ccgaccacca catcacacat
     2101 tcaatgagcc aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca
     2161 atcaaatgag aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta
     2221 atccaagatt atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg
     2281 actccttgac aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg
     2341 ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac cttgaaatcc
     2401 tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt
     2461 ttaccaaaga gttactagat attctagacc taaatcttac ctttcatgaa gatgcctttc
     2521 gcaaagagcg cattaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac
     2581 cagaggaatg cttccattgc cactatcaaa ataagcaaac gatcattaaa tggggctgga
     2641 aaaaagtatc catcttattg aatgatgtca gtaactacaa gaccattgtg cggattaaca
     2701 aacagcgctt caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagcg
     2761 atcgtcgctg ctccattgcc cgcagagtca aacaagccat tcttgagcta ttaagtgagc
     2821 ctatttctat gtctttgatt gcacgtatga agcacatttc tccgaccact gtcattcgaa
     2881 tacttcgcag ccttcggcct aaaa
//
LOCUS       DADQRX010000185         2694 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.194, whole genome shotgun sequence.
ACCESSION   DADQRX010000185 DADQRX010000000
VERSION     DADQRX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.194"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            61..384
                     /locus_tag="J8V14_002794"
     CDS             61..384
                     /locus_tag="J8V14_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922856.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            548..802
                     /locus_tag="J8V14_002795"
     CDS             548..802
                     /locus_tag="J8V14_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922857.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            872..1117
                     /locus_tag="J8V14_002796"
     CDS             872..1117
                     /locus_tag="J8V14_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0922858.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1193..1558
                     /locus_tag="J8V14_002797"
     CDS             1193..1558
                     /locus_tag="J8V14_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922859.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1619..2182)
                     /locus_tag="J8V14_002798"
     CDS             complement(1619..2182)
                     /locus_tag="J8V14_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922860.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          789 a          475 c          423 g         1007 t
ORIGIN      
        1 tgttacttta atttttattt gcttatttta gaaaaaatgt tattctttta tttattctca
       61 atgttaggag tgattatttt gccatttatt gcgattttat tcgatttagt tgcggcagca
      121 gcttattttc tccaattaaa ttatcaaacc tcaggaattc tgtttattgg aatgattttt
      181 caaggtgtga ttaccctttt gcttcttgtc atgatgctca gctataaagg aaaaagatat
      241 gcccgtattc aaacaaaagt atttgttaaa tatgtttcca ttcgctatgg gatcatcatt
      301 ctttcctttt tagtgaatgc gatcgtcctc tttttatatg tattgaatta cttagatatc
      361 aatccattga ttttttctcg atagataaga cacattcagc ggaacagttc ctgcttatca
      421 gcagaaaact gttctgcttt ttcatttttc attgatctat tcttctttta ttttcctagc
      481 atttcatttg tgatgtatgc aataacatgt tatcttttat tatatgaatc gttgaggagt
      541 ccgttacatg attacgttgg tgagagtttt gttttgggtg cctgcggtcg cactggtcgc
      601 aagtattgtt tatcttatga attggaacaa agaacggttt tatttagcta ttttgactct
      661 tccggctatc tactttatgt ggaaagtttt taattacaac tactttgaac ccgatagtgt
      721 ttttatagaa gaattatcag gactggtttt gtctttattg atcgtcattc tttatttgat
      781 acgtttaaat aaaaaacatt gagtactatt gattgcatgt gtagctttct atagtcatta
      841 taactattct actataatga aaggtgatta aatgagtatc gaagaagcta ttttactgga
      901 tactttacgt tgtcgtctga atttcttaag aactggccga tctttatctc aagtagctgt
      961 ttctagcgcg ttaagtattc ctttattcaa atatcgtaag tacgaagaag gagaagaaat
     1021 tccaagcatc attgaattaa tctatatcgc tgacttttac gatatttccg ttgattacct
     1081 aattggtcgc tcagaaacag caagcaaact gaagtgattt tttccagcct tggtgctggt
     1141 tttttcattt aacgtctaag gttaacaatc tatatataaa ggagacattt ctatgcgatt
     1201 atggcatgaa aaattgattt caaagcttcc tcgtcaacag cttttaggac aacatcggga
     1261 atgctgtgca ttaagaggaa atggctgggg aaagaaacat gctactgtgg attatgtttt
     1321 tcactattct ccttatagat tattccaata tcatcagctt gtcatggatg aaatggaaaa
     1381 acgtggttac agcccagcac cagagtggaa aaatcctatg tatcgtggca aatcatgcga
     1441 agcatttttt gatttaccta ctgtagagcg caacgtgccg atttatccgg aacacgatga
     1501 agtttatcta gcagaatgtt tagcaaattt ggagcaaaaa ggaatttatc ttacctaata
     1561 taactagaaa agccttctat ctcctcttac ttcattaaaa agctttaacc atcttagatc
     1621 aagctaaaat gtcgtaagtt ttagggttat cttcatcctc acgtgcaaaa ctaaaagcgt
     1681 actctagttt tggttcatcg ccaacttccc tagacataaa tgaacatctc ctgacgtggt
     1741 ttaaaagtac cgcatcttcg acgtctgtat acgatgcaca gcacagattg cttcttttga
     1801 tctttctttt gatttttctg ttgcttcttc tacaccttct tcgattacta catcgatgct
     1861 tacaattgct tctgcctaat attcccatgc atcgtcgatt taagcctctg tagtcactgt
     1921 gatcgtttcc acattttaac tcctctgtcg tggcttcagt tgattttgtg atcagttcag
     1981 ttgctaagcc agagtcagaa caacctgcca ttgctagcgt taatattgat agtaaaaata
     2041 gtttttttat cataaggttc cccccctgaa aatagactaa aataataacg ctttcttttt
     2101 ttgttttcag acgaaaatta ataatttcta aatattcttt aaaaaagctt ataactatta
     2161 tgcctttttt aatatataac acagttattt ttaaaaataa gaatgctaaa tcactctaca
     2221 ataacggcga aagcacaaaa aataaaaaaa ttattacgtt attaaaaaaa cagatgaaag
     2281 atcaggtttt attagatctc attctttcat cacttgaagg gatttaatga actacttttt
     2341 cctctttgtc gtttttatat attcgtattc cttcaaacag taggatagcc tctttcaatg
     2401 cttgatattt gaactgtccg ttacttaaaa atttagatgt gccgccctct tcctagaaca
     2461 tattcttttt tactttttca ttaattgaca gaccaatcga acgaaactgt aaatttcgat
     2521 aggataatga ttggtcaaac tcattcattt tcaagacatc tcgattcata ttcaagatag
     2581 gaatcttttt atcaacatca ctttattcgt attttcctct accataaaag cttcacgctc
     2641 cacaaaattg ttttatacac tactattcag cccattcaac gaccaatgaa tttt
//
LOCUS       DADQRX010000186         2613 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.195, whole genome shotgun sequence.
ACCESSION   DADQRX010000186 DADQRX010000000
VERSION     DADQRX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.195"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..1131
                     /locus_tag="J8V14_002799"
     CDS             4..1131
                     /locus_tag="J8V14_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0922861.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1349..2491)
                     /locus_tag="J8V14_002800"
     CDS             complement(1349..2491)
                     /locus_tag="J8V14_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0922862.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2496..>2613)
                     /locus_tag="J8V14_002801"
     CDS             complement(2496..>2613)
                     /locus_tag="J8V14_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBA0922863.1"
                     /translation="KECKELLVEKGYFFYRNSKIKRIPISILEEVLHISWEE"
BASE COUNT          871 a          474 c          419 g          849 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat caatgatgat
       61 ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc cgaagctgct
      121 ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg tacggatact
      181 cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa tctaaaatac
      241 aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta tttaggaaag
      301 aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc tgaatttaaa
      361 aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa tgcgctgaat
      421 atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt aaaaatacca
      481 aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct tgaggacttt
      541 gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt gaatattgga
      601 ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga tgtcgatttt
      661 tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc atgggtatat
      721 ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac cttgctagca
      781 attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg aaagctctac
      841 attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc aagtgtagtg
      901 aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt ccattctctc
      961 agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga aatctctgaa
     1021 cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt aacagataag
     1081 atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg atttttgcca
     1141 ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg tttatttttt
     1201 acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca ttttcgatac
     1261 cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata ggctatgaca
     1321 gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt tcatttgcga
     1381 gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt ttaatagagg
     1441 catgacctag acgcttagat attgcataga tatttttatt atgttcaatt aaaaaggcaa
     1501 catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt tcagctagtt
     1561 tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa gaaaaaacca
     1621 attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa tcttcaacgg
     1681 tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca gcaaattcat
     1741 aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca tttattaagt
     1801 caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta acaaaaataa
     1861 aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca ctattatata
     1921 ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt tttattttac
     1981 ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt ttaaaaacta
     2041 ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc tcaatgccct
     2101 tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga attggtcgaa
     2161 tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata tgatctgttt
     2221 tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac tctcgataaa
     2281 aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa ctttgtttta
     2341 actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg gtcacttgaa
     2401 ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg cctctttttc
     2461 tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga aatatggaga
     2521 acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata aaaaaagtat
     2581 cctttctcaa ctaaaagttc tttacattcc ttg
//
LOCUS       DADQRX010000187         2418 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.196, whole genome shotgun sequence.
ACCESSION   DADQRX010000187 DADQRX010000000
VERSION     DADQRX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.196"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1149
                     /locus_tag="J8V14_002802"
                     /pseudo
     CDS             5..1149
                     /locus_tag="J8V14_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="J8V14_002803"
     CDS             1320..1778
                     /locus_tag="J8V14_002803"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBA0922864.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="J8V14_002804"
     CDS             1937..2407
                     /locus_tag="J8V14_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBA0922865.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DADQRX010000188         2398 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.197, whole genome shotgun sequence.
ACCESSION   DADQRX010000188 DADQRX010000000
VERSION     DADQRX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.197"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="J8V14_002805"
     CDS             207..2069
                     /locus_tag="J8V14_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922866.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="J8V14_002806"
     CDS             2069..>2398
                     /locus_tag="J8V14_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0922867.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DADQRX010000189         2376 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.198, whole genome shotgun sequence.
ACCESSION   DADQRX010000189 DADQRX010000000
VERSION     DADQRX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.198"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..428
                     /locus_tag="J8V14_002807"
     CDS             33..428
                     /locus_tag="J8V14_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922868.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            753..1370
                     /locus_tag="J8V14_002808"
     CDS             753..1370
                     /locus_tag="J8V14_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922869.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1411..1710
                     /locus_tag="J8V14_002809"
     CDS             1411..1710
                     /locus_tag="J8V14_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922870.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1744..1911
                     /locus_tag="J8V14_002810"
     CDS             1744..1911
                     /locus_tag="J8V14_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA0922871.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1956..>2376)
                     /locus_tag="J8V14_002811"
     CDS             complement(1956..>2376)
                     /locus_tag="J8V14_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922872.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          839 a          351 c          453 g          733 t
ORIGIN      
        1 cgataaaata agtatatgaa ttgtaaaagg acatgatgat tatggaagaa atagaatgga
       61 ataatctgac tcaaaataat aaaaataatt ctggatgtgg aattatcttt atgtttgtta
      121 tagttttggg aatagtagta cttttaataa ctgtcaagat agtagattac tttgcgaata
      181 ctcctgaaga acaaatacag agaaaacttc ctgagtttga aagagtacca ccttatgatc
      241 caagagataa ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa atgtggtttg
      301 aagttacagt tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat tatattgtaa
      361 aggcaaaagt atcaaaaaga tttattttta accctaaatt agaaatatat agtgttgtaa
      421 tagaataata gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg atgtgaaaaa
      481 taaaatatac catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga
      541 ccgttaaggc ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt
      601 aaacttactg tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat
      661 ggcataataa ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa
      721 caattttagt gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca
      781 gtagttatga tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg
      841 aagaatatat atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat
      901 taatgaaacg gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag
      961 gcagaaataa acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg
     1021 atatttctgt gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt
     1081 tagagacgct tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc
     1141 ggaaacgaat taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa
     1201 aattcggcag aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga
     1261 aagcaggaaa gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct
     1321 tttatcgacg ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac
     1381 agctattaga cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt
     1441 actggaacgc tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac
     1501 ggccattgtc aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct
     1561 aagtcgttgt ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt
     1621 gcgttaggtc atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa
     1681 cagttgaaag aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt
     1741 tttatgaagc gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat
     1801 attgcttttc acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat
     1861 tacttttact gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat
     1921 agtgcggtag aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt
     1981 ttataaatag ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct
     2041 tgctttaaca tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta
     2101 atagaatact cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct
     2161 tgccgctgac gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat
     2221 aaaaacatat cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac
     2281 tgtgcatcta atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt
     2341 atatcttcat aatctcttcc taaacgatct agtgat
//
LOCUS       DADQRX010000190         2364 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.199, whole genome shotgun sequence.
ACCESSION   DADQRX010000190 DADQRX010000000
VERSION     DADQRX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.199"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..586
                     /locus_tag="J8V14_002812"
     CDS             <1..586
                     /locus_tag="J8V14_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HBA0922873.1"
                     /translation="IKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMI
                     KLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHT
                     LFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIK
                     ESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            579..2006
                     /locus_tag="J8V14_002813"
     CDS             579..2006
                     /locus_tag="J8V14_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBA0922874.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            2008..>2364
                     /locus_tag="J8V14_002814"
     CDS             2008..>2364
                     /locus_tag="J8V14_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922875.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNY"
BASE COUNT          826 a          280 c          372 g          886 t
ORIGIN      
        1 aattaaacat aaagaatttt attataaaca tatagatgta tatcaaaaac atgattatcc
       61 tagaattaat aggaataaca caactgcatt taaaattttt agagctataa ttaatcgttg
      121 tctgaggatg attaaacttc ccccaaaaat tagacaccat aaatttgatt tgtatcatgg
      181 ttctcaatgg tgggctttgt cgaaagaatg cctaacagaa ttaatacaaa tgtatgaaca
      241 aaatcaacaa gactacctga attttaaaat aggtatgttt gccccagatg aaaaattttt
      301 tcatacactt ttttttaatt cctcgttcaa aaataaaaat gttattggag gaccagattt
      361 accacttgaa ttgaaaaata ttgaagaaac tactttacaa acttcgaaat tagcaaatat
      421 tcatattatt gatccttcca tgaacaaatg gtttgatgaa agggatatag atcaaataaa
      481 agaaagtgaa aaattatttg taagaaaagt taaatcggat tactctgata aactattaaa
      541 tagaattgat aaagaaattt tgaaagttgg aaatatagat gaatagaata gtacctgttt
      601 tagagaagag tttattaagt cgagatgcgt ttcgagtaat aaatttaatc atttgtctgc
      661 tttattttat gcctagtact tttaatgtaa ctaatatccc gatgaaaata gcttttgctt
      721 ggggtggaat tatagttttg tatgaggttt ttataaagaa aaattttttt aaaatgcgtt
      781 tttcttggct actgattgga gcaatgatta gttttatctt aactataatc attaacagag
      841 aaaatttttt aattccatct atttataatt ttggatattt gctgattact ttgatagtaa
      901 tttttcctgc agacttttca caaagtgaag aggaaaaaaa gaaaaaaatg gtgtatttta
      961 ataatgtttt cattatcata atttttttag ctgcgctaat atctatctat caatttattt
     1021 ttttgatttc ttatcatgtc ccaacaggaa cgccaggatt acttgcaaga caaggtttta
     1081 ttgaacatcg gttatttggt gtctatacaa gtccaaatgt tggagctatg tttggatata
     1141 cttctattat tttaagttta attagtgtat taatttcaaa aataacaaaa acgattcgtg
     1201 ttttttatat gatgaatttt atcgtacaaa tattttatat tacactttct tcgtctagag
     1261 gtgcccaggt agtaatttgt tcttttttag tattatttct tttgttgttt gccaattcaa
     1321 aatttagaaa gagcattaga gaagtactac ctgtaaaaat caaatggatg gtaagcatat
     1381 tattagttct attggtatat ttttcttttg gtactaatta tgctaaggat tttttagcaa
     1441 caattcctgt atcgattgat agaaaagtag aagttataga gtcaaatgaa tcagataaaa
     1501 ctccacttcc agaaaaagaa gtaatattac agcattctag tgataactcc gaaatttcag
     1561 ctggtcgttt tacgatttgg aaagctgcgc ttaaagttat aagaccaact ttgttatttg
     1621 gttatggaga gaccgatttc tatgggaata gcgatgcaaa atttttgaaa aaagtagatc
     1681 ttgatactac agatgtgaat gaactaaaaa gagcacatgg aaacatgcat aatggatttc
     1741 ttcaagtact tgtatcaagt ggtataattg cttttatatg tatctatgct ttctatgtgt
     1801 taaatatctt tagtttactc aaaacatttt ttagaagtaa agtgaattat gcgtttcttg
     1861 gtattgtttt aatttttata ctatctattt ttatagatga aatggtggag gctcatgtac
     1921 ttttcaataa gcgcgatgtc atttcaattg ttttctggta ttatttggga agcatttctt
     1981 acttgtattt aaaaaaggag aattaatatg aagacagctt tcattgcttg gactccttta
     2041 catttgatta atatattaaa tgtttatcaa aatttttacc gagaatcaaa aggagattta
     2101 tttatttatt ctgaatttga aaacgctaga agatatagtg agcgtataaa agaattaggg
     2161 ctatttaatg atgttgtttt tgtgaatcac cggaaaatcg gttcttcctt taaacgtaaa
     2221 attaacttgt taactaacca taatcaattt ttctcggatg atttatccgt ttatgatgaa
     2281 atttttatac agggggaaaa ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa
     2341 aatccagatg taaaactaaa ttat
//
LOCUS       DADQRX010000191         2262 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.200, whole genome shotgun sequence.
ACCESSION   DADQRX010000191 DADQRX010000000
VERSION     DADQRX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.200"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(856..1386)
                     /locus_tag="J8V14_002815"
     CDS             complement(856..1386)
                     /locus_tag="J8V14_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBA0922876.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1383..2000)
                     /locus_tag="J8V14_002816"
     CDS             complement(1383..2000)
                     /locus_tag="J8V14_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBA0922877.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          737 a          435 c          243 g          847 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttgggttt ctttatattt tcatatttaa tagacattac catataaaca
     1621 tatggaataa aaagaataat attttctaaa cacgctgtag ataactcttt tgttccagtt
     1681 gttgtattat acttataaat ccaccagtct cccataacat cagataaagg atttaaccaa
     1741 attgttcgat tgattaatgt tgtaaataaa atcatcgcag tatagaatat caaagcacaa
     1801 acttttctat attttctatc cttctttaaa tgaagataaa gttccattag tatacgttta
     1861 tagccctcac ctgcagcaat gccatctgtt gcatacataa aatagaccag tcctaaaatt
     1921 gtcaataaaa cagcaaaccc aaagggttca tataatttat ataatatatc tgtaataatt
     1981 tctgcaatca ctattatcat aattatttct ccaatatcaa gtatacactt atcgcttcca
     2041 attacacatg atcatattag agtgtattat cacattctct aatattcaat ttatttttcc
     2101 taaaatattc gaaataatcg tcttgataac gagtaagtat ttttatacga ttgataacat
     2161 agtaacaata tttttaatta tagtaaaaaa tcatctgcct ttttcatcca cctaatctta
     2221 tttttcattt tattcaacat ccttaaataa acttccgttc ct
//
LOCUS       DADQRX010000192         2259 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.201, whole genome shotgun sequence.
ACCESSION   DADQRX010000192 DADQRX010000000
VERSION     DADQRX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.201"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2145)
                     /locus_tag="J8V14_002817"
     CDS             complement(148..2145)
                     /locus_tag="J8V14_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBA0922878.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DADQRX010000193         2227 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.202, whole genome shotgun sequence.
ACCESSION   DADQRX010000193 DADQRX010000000
VERSION     DADQRX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.202"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..280)
                     /locus_tag="J8V14_002818"
     CDS             complement(<1..280)
                     /locus_tag="J8V14_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922879.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="J8V14_002819"
                     /pseudo
     CDS             742..2124
                     /locus_tag="J8V14_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DADQRX010000194         2218 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.203, whole genome shotgun sequence.
ACCESSION   DADQRX010000194 DADQRX010000000
VERSION     DADQRX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.203"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..1598)
                     /locus_tag="J8V14_002820"
     CDS             complement(51..1598)
                     /locus_tag="J8V14_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBA0922880.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="J8V14_002821"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="J8V14_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBA0922881.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          622 a          464 c          397 g          735 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaa
//
LOCUS       DADQRX010000195         2205 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.204, whole genome shotgun sequence.
ACCESSION   DADQRX010000195 DADQRX010000000
VERSION     DADQRX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.204"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="J8V14_002822"
     CDS             complement(<1..914)
                     /locus_tag="J8V14_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBA0922882.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="J8V14_002823"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="J8V14_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBA0922883.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          747 a          384 c          421 g          653 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtata
//
LOCUS       DADQRX010000196         2019 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.205, whole genome shotgun sequence.
ACCESSION   DADQRX010000196 DADQRX010000000
VERSION     DADQRX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.205"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="J8V14_002824"
     CDS             100..570
                     /locus_tag="J8V14_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBA0922884.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="J8V14_002825"
     CDS             792..1895
                     /locus_tag="J8V14_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HBA0922885.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DADQRX010000197         2009 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.206, whole genome shotgun sequence.
ACCESSION   DADQRX010000197 DADQRX010000000
VERSION     DADQRX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.206"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="J8V14_002826"
     CDS             200..742
                     /locus_tag="J8V14_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922886.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="J8V14_002827"
     CDS             898..1347
                     /locus_tag="J8V14_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBA0922887.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2009)
                     /locus_tag="J8V14_002828"
     CDS             complement(1377..>2009)
                     /locus_tag="J8V14_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HBA0922888.1"
                     /translation="GLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          729 a          313 c          381 g          586 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatcc
//
LOCUS       DADQRX010000198         2003 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.207, whole genome shotgun sequence.
ACCESSION   DADQRX010000198 DADQRX010000000
VERSION     DADQRX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.207"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="J8V14_002829"
     CDS             <1..378
                     /locus_tag="J8V14_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBA0922889.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="J8V14_002830"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="J8V14_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBA0922890.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="J8V14_002831"
     CDS             1859..>2003
                     /locus_tag="J8V14_002831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA0922891.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DADQRX010000199         1979 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.208, whole genome shotgun sequence.
ACCESSION   DADQRX010000199 DADQRX010000000
VERSION     DADQRX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.208"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="J8V14_002832"
     CDS             complement(325..528)
                     /locus_tag="J8V14_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922892.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="J8V14_002833"
     CDS             complement(728..1744)
                     /locus_tag="J8V14_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBA0922893.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DADQRX010000200         1942 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.209, whole genome shotgun sequence.
ACCESSION   DADQRX010000200 DADQRX010000000
VERSION     DADQRX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.209"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="J8V14_002834"
     CDS             132..344
                     /locus_tag="J8V14_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922894.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..721
                     /locus_tag="J8V14_002835"
                     /pseudo
     CDS             506..721
                     /locus_tag="J8V14_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            800..1926
                     /locus_tag="J8V14_002836"
     CDS             join(800..1028,1028..1926)
                     /locus_tag="J8V14_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA0922895.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          729 a          300 c          336 g          577 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 tggtgttagt atcaaatctt agacagcttt tcgtagagac aagaataaat taaacttatc
      781 tacgagagga tgaattttta tgacccgata tgaagaaaat tttaaacaaa tgattgttga
      841 actgaatcaa actggacgtt ctgttcgagg gttagcgaaa gaatatggct tatctgaagc
      901 aacgatttac aaatggaaga atttatattt acctgatcag tccacaggac tgactggaaa
      961 agaagtagct gaactgagaa aagaaaatgc tcgtttaaat gaggaacttg aaatcttaaa
     1021 aaaagccgca gccatattct ctcggaaaac ctaaattcgc ttgttcaatt tattgaaaaa
     1081 tggtgtaagg attacactgt ttctttgtta tgtcggttat tagaaatccc tagaagtgtc
     1141 tattattttt ataagaataa accgttgaca gctacagaaa tcagaaataa taagttgaaa
     1201 aagaagattt caacaatttt ttttacgaat aaacaacgct atggtgccac aaagatccat
     1261 caagttttat taaaagaagg gatttcagta tctcttaaac atgtccaaaa gctaatgaaa
     1321 caattaaatt taaggtcgat tgtagttaaa aaatatagac ctcaaaggtc taataaaccg
     1381 atcatttcaa aagagaatct cttaaatcag gacttttcta ctgaaacgat ctgtgagaaa
     1441 tgggcagctg acattacgta cattcctact aagaaaaatg gttggtgtta cttatcttca
     1501 atcatggatt tacacacgaa aaaaattatt agttatacat tttcaaaacg aatgactgtg
     1561 gattgtgtca ttcaaacatt gaataaagca aaaatacatt atcacattcc agaaggaatg
     1621 attctacaca ctgacttggg cagccaatac acagcaagag aagtggaaca atggcttaaa
     1681 accaacaaaa taaggcattc ttatagtaga aaaggaacac cttatgataa tgctggaatc
     1741 gaatctttcc atgcctcatt gaaaaaagaa gaagtctaca cgactagcta ctcagatttt
     1801 gaagaagcaa atcgagcgct atttagctac attgaaggat tttataaccg aaatcgaatc
     1861 catagctcga ttcactatct aaccccacag gaatttgaag aattagcaaa agaaaaaatg
     1921 gcttaaccgt ctctactaaa at
//
LOCUS       DADQRX010000201         1831 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.210, whole genome shotgun sequence.
ACCESSION   DADQRX010000201 DADQRX010000000
VERSION     DADQRX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.210"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..1627)
                     /locus_tag="J8V14_002837"
     CDS             complement(161..1627)
                     /locus_tag="J8V14_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA0922896.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          572 a          364 c          307 g          588 t
ORIGIN      
        1 gctgaccttc agcattagct caaaaaacgg caaaatatga ggagctattt ttgtttggct
       61 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccagaa
      121 gtagcttctg tcacaacctc ttgtaacaag tatttctatt ttaaggctga accactgttt
      181 ctggttcagt aatttctttg tttttgtctc gacgagcaat tgctggcaag atcataccta
      241 ataagatcaa gacaattggt gtgatgatat tcgtgaataa ttggaaccac catgctttag
      301 gatcagcagt ataatctact tttggcacca ttcctaggat acatgcaaac gcagtgaagg
      361 caaagcacca agcaccaaag ataaaaccga tttttggatt tttaacaaat ttatattcag
      421 aattgaattt cttataagct ttattcaaca tcatatatgc caagaacacc cataggtaac
      481 gcattggcat gacaactgag ttcaagttcg tcaaccattt tactaattca ttcatatctt
      541 tgataccaaa gatcggcaac aaaataatga ttcctactag tatacctgtc agaagataac
      601 cattcaccaa tgtaccttta cgattacgtt tacgtagcca gtttggtaca aattctggat
      661 ccgcatctgc taacaagatt tttagtggtg catcaattga aaaggctaat gcggagattt
      721 gtccaatcat gtttgtcaat gcgtaaatga tcatcaatga gttaccgata ccatagtaat
      781 tacctaaaat ttgaaaagca ctgtatgccc catttgccat caagtcttta gggatattat
      841 cgctagcaaa cagcataccc attgcgactg agcctaatac agcacaaatc cctaccattg
      901 ctgctaagaa gatcatccct ttaggaaatt cctttgcagg atttcttgta gagttgacat
      961 aaggggatat tttttccgcc cctccaactg caaatactag catcgaaacg gttgtgaaat
     1021 aactgaaatc aaatgttgga atatatgttt tcacttgatt catatgtggt gtggcaaatt
     1081 ccatcgttgg attgatgaac ggtgccgtta cagctaaaag aataaataag atcgacatga
     1141 cgaacatcgc tgttccggcc agtccgccaa tcactttcaa agtagaaagt ccttttgttg
     1201 ataaatataa aaaggcaaga aatataataa gtgaaataac tgctaccatt tgtactgaca
     1261 tcgtatttac gatatttcca tttccttgga ctgcccaacc accagcaatc agaattgctt
     1321 gaggcttttg tgccaaataa ggaatatgga cgacccagta agtccaagcg gcatagtatg
     1381 ccagtcgttt attagatgtc gtattttcga cccatgaact gacgcctcct ttactatcct
     1441 tgaaggctga tcctaattgt ccaacgatca atgcgtatgg aataaaataa atggccaaaa
     1501 tcaatagcca agaggtgaca actgatactc cttgctgagc atagttatta actacattcc
     1561 ccatgcccca aaccatgtta aatgcaatta acgcaacagt aaaccagcgt aattgtttgt
     1621 tgttcattcg ttctctcctt tatgataaaa atttgttatc cagatgtaat catattcaat
     1681 ttttaacaaa cttacaagac agtgttattt tatgtgtgta ttcttttatt taatacacat
     1741 cgttttttca tattttttcc atcaaaaaaa taagttattt tcttttattt cattgacctt
     1801 attgaatttc aagctatttt gaattttctg c
//
LOCUS       DADQRX010000202         1817 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.211, whole genome shotgun sequence.
ACCESSION   DADQRX010000202 DADQRX010000000
VERSION     DADQRX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.211"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(485..1057)
                     /locus_tag="J8V14_002838"
     CDS             complement(485..1057)
                     /locus_tag="J8V14_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922897.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /locus_tag="J8V14_002839"
     CDS             complement(1073..1678)
                     /locus_tag="J8V14_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBA0922898.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          535 a          367 c          270 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtct
//
LOCUS       DADQRX010000203         1674 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.212, whole genome shotgun sequence.
ACCESSION   DADQRX010000203 DADQRX010000000
VERSION     DADQRX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.212"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            243..1634
                     /locus_tag="J8V14_002840"
     CDS             243..1634
                     /locus_tag="J8V14_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBA0922899.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
BASE COUNT          573 a          267 c          287 g          547 t
ORIGIN      
        1 ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata
       61 attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa
      121 ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt
      181 gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaatta aactatagaa
      241 agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac
      301 ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa
      361 tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac
      421 atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca
      481 gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa
      541 aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag
      601 aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg
      661 agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt
      721 tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga
      781 tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg
      841 tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg
      901 tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat
      961 tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat
     1021 tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt
     1081 cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc
     1141 aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa
     1201 cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag
     1261 aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta tctagctata
     1321 tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac
     1381 tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata
     1441 ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga
     1501 aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg
     1561 ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct
     1621 tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaa
//
LOCUS       DADQRX010000204         1671 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.213, whole genome shotgun sequence.
ACCESSION   DADQRX010000204 DADQRX010000000
VERSION     DADQRX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.213"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..978
                     /locus_tag="J8V14_002841"
     CDS             4..978
                     /locus_tag="J8V14_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HBA0922900.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          586 a          219 c          267 g          599 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat t
//
LOCUS       DADQRX010000205         1643 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.214, whole genome shotgun sequence.
ACCESSION   DADQRX010000205 DADQRX010000000
VERSION     DADQRX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.214"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            306..560
                     /locus_tag="J8V14_002842"
     CDS             306..560
                     /locus_tag="J8V14_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA0922901.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            561..878
                     /locus_tag="J8V14_002843"
     CDS             561..878
                     /locus_tag="J8V14_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA0922902.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          570 a          250 c          316 g          507 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaatggatgt
      121 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
      181 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
      241 cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa aaggagtgtg
      301 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
      361 caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa ggcaacttat
      421 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
      481 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
      541 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agttttcaga
      601 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
      661 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
      721 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
      781 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata cttgtaggac
      841 gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga aaaagtcgct
      901 catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt cggttactcg
      961 aagattgata tataagactt agtacctttt tattttgctc catgttatgg ccgaaggtct
     1021 aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg gggatcgctt
     1081 ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc tctattccca
     1141 aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc aaaattgacg
     1201 taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt taaaagagga
     1261 agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa aatatacatc
     1321 cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa aggaatggga
     1381 taaaatatag gtacaataac tatttgatgc taaatataat taatggagga gagatagtga
     1441 acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt actactttca
     1501 aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt
     1561 ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt ctttctgaga
     1621 gtaggctttt tcattttaga ata
//
LOCUS       DADQRX010000206         1636 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.215, whole genome shotgun sequence.
ACCESSION   DADQRX010000206 DADQRX010000000
VERSION     DADQRX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.215"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1502)
                     /locus_tag="J8V14_002844"
     CDS             complement(183..1502)
                     /locus_tag="J8V14_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBA0922903.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DADQRX010000207         1594 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.216, whole genome shotgun sequence.
ACCESSION   DADQRX010000207 DADQRX010000000
VERSION     DADQRX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.216"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="J8V14_002845"
     CDS             288..695
                     /locus_tag="J8V14_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922904.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="J8V14_002846"
     CDS             709..1110
                     /locus_tag="J8V14_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922905.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="J8V14_002847"
     CDS             1112..1483
                     /locus_tag="J8V14_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922906.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          520 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DADQRX010000208         1586 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.217, whole genome shotgun sequence.
ACCESSION   DADQRX010000208 DADQRX010000000
VERSION     DADQRX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.217"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..585
                     /locus_tag="J8V14_002848"
     CDS             <1..585
                     /locus_tag="J8V14_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HBA0922907.1"
                     /translation="KMAKVLRRSKIDSDTLRKAFFYAQEAKISIIASRETQDDQLTFD
                     ESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALTDKSEEKLQKLQRDLQAD
                     GIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMAFGDQENDIAMLNYSGLS
                     IAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            625..1122
                     /locus_tag="J8V14_002849"
     CDS             625..1122
                     /locus_tag="J8V14_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA0922908.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1507..1580)
                     /locus_tag="J8V14_002850"
     tRNA            complement(1507..1580)
                     /locus_tag="J8V14_002850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1544..1546),aa:Ile,seq:cat)
BASE COUNT          548 a          296 c          291 g          451 t
ORIGIN      
        1 aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag aaaagccttc
       61 ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca agatgatcag
      121 ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca tttcaattta
      181 cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct tagctatctt
      241 aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag agatctgcaa
      301 gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac gcctacaggt
      361 atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga atcgagtgac
      421 atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag cgggttgagt
      481 attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt aacactgacg
      541 aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga ataaaatgac
      601 gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa tcatattctt
      661 gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg tattgctcaa
      721 agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga tataccagtt
      781 gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct tctttctcca
      841 caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga aactttagct
      901 gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga agtagtcggg
      961 attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc ttttcctgaa
     1021 gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga atccgcttta
     1081 gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct atcttcattt
     1141 acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta cgtgcttttt
     1201 ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc acgacctctt
     1261 tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg caaaatatga
     1321 ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt tcacaacctc
     1381 ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca agtaaaaaca
     1441 taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
     1501 ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc gtctgctcta
     1561 accaactgag ctaaaggtac atcata
//
LOCUS       DADQRX010000209         1571 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.218, whole genome shotgun sequence.
ACCESSION   DADQRX010000209 DADQRX010000000
VERSION     DADQRX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.218"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            61..1527
                     /locus_tag="J8V14_002851"
     CDS             61..1527
                     /locus_tag="J8V14_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBA0922909.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          437 a          291 c          330 g          513 t
ORIGIN      
        1 aaatttttcg gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt
       61 atggaaaaac agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc
      121 ttcactgaga tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat
      181 atgtacgata cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc
      241 atgtcgatct acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac
      301 cgagtgttag gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac
      361 atcgtcttgg ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt
      421 atcggaaccg cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag
      481 actgacttgc gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca
      541 ttattagcac cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc
      601 tctttagctg ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag
      661 acattagacg gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga
      721 ttcatccgta acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa
      781 ctaactggtc atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc
      841 ttaccgttgt attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa
      901 ccaagagtat gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa
      961 gaacaaggtt catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt
     1021 ggtgtaacga tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg
     1081 acaccagtat tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg
     1141 aaattctctc ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt
     1201 atgctttatg gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc
     1261 gtggttattg ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca
     1321 ccacgagcat ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa
     1381 gcaatcaatg cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt
     1441 attatcggtg cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc
     1501 aaaaaactaa tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg
     1561 cagcagttcc t
//
LOCUS       DADQRX010000210         1561 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.219, whole genome shotgun sequence.
ACCESSION   DADQRX010000210 DADQRX010000000
VERSION     DADQRX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.219"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..632)
                     /locus_tag="J8V14_002852"
     CDS             complement(<1..632)
                     /locus_tag="J8V14_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBA0922910.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLR"
     gene            complement(722..1402)
                     /locus_tag="J8V14_002853"
     CDS             complement(722..1402)
                     /locus_tag="J8V14_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBA0922911.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT          416 a          333 c          289 g          523 t
ORIGIN      
        1 cgaagctgaa cagttggctt tgcgttgacc tcgtccaaat ctcctgataa aagtttcaga
       61 tccggttcta tcgtctcttg actttcttgt aaaacgactg cgagattata agcaatacga
      121 tttttgattt gtgctccttc tgtagcattt tctgtttcag cttcttcttt aggtgaaaca
      181 tgaatacccg ctaatacacg tcccttaaag ctttttgctg ggattttcac ttctataggt
      241 acagttgttt ctgcatgtgc tgatacattg atttcctttt tcgcaatgag caaaacgtca
      301 cggacatcaa acggcaatgt ctcatctaac gttgggtttt tttgactata atcgatcgtt
      361 ccaccatcac tcgttaccgc cggattgaca gagacctcaa atgttcgttc ttcatctgaa
      421 gaattgctga taattacttc tagtgtctga ttttgttccg gtgccactaa taaatcaaaa
      481 taaccgattg ctttatctat ttggttctct ggaaataccg gtttgattcc aaagtctcct
      541 gctgaatcct ttgcttggct gattgacgga aaaatgaaaa atagacttaa aatagaaaaa
      601 aacgaaacaa acagtcgttt actttgattc attgtccacc acatcctttc aacaacaact
      661 gcggtaaaaa atccttcacc tatagtattt tcaccgcagt ttgttgaatt tatttcatac
      721 tctatgctac tgggccagcc acgaggttcc aagtcaaatc agccgtgaat gttccagctg
      781 ctgctttcgg cactttcagt gtgatatttg ctggatcata aacagaaagc catgttccca
      841 gaccttgatc ttttgctgct gtaaatattg gtgtcgctgc acttgcttta ctcaacccag
      901 ttacagtttg actagttggt ccagtggatt cgttatctga cgtactttta acagtggatg
      961 ttccaaaatc taattcagcg ccttgcaaaa cttgtgaacc gtttacaaaa tctgaaaccg
     1021 aaactgtcac gttccaccct tgtgcttggc catctgcacc tacacctcga cgatctgata
     1081 cttggagata aggtgtatcg ttttttgaag cgtctacttg atacgttttt gttccgcttt
     1141 caatctcgtg tgtaccaaat ggcaattcgg gaactacatc cagtgttaaa ggacctgtct
     1201 ggccagttgc tggattttcc ggatctgctg gctctactgg ttttgaagga tcagtagggt
     1261 taagaggctc cacggtattt gttggttcag taaaggtaat atctcctttt gaagtcgctt
     1321 gaccctcagc aaaagctggt gttgccaagc cgcctagaag aagtgttgaa agtaataatg
     1381 tcccaatcat gttctttttc ataataaatc actccttttt cttagtgacc taagtatagt
     1441 agagacagta tgcaaaaaat cctcatgaat tcatccgctt acttgaaatt atgacccttt
     1501 ttgtataaaa gagaaaaaaa caataaaatc gtttacatga atattcttcc ttttttatta
     1561 a
//
LOCUS       DADQRX010000211         1536 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.220, whole genome shotgun sequence.
ACCESSION   DADQRX010000211 DADQRX010000000
VERSION     DADQRX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.220"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="J8V14_002854"
     rRNA            complement(<1..>1536)
                     /locus_tag="J8V14_002854"
                     /product="23S ribosomal RNA"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DADQRX010000212         1512 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.221, whole genome shotgun sequence.
ACCESSION   DADQRX010000212 DADQRX010000000
VERSION     DADQRX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.221"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..403
                     /locus_tag="J8V14_002855"
     CDS             5..403
                     /locus_tag="J8V14_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBA0922912.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="J8V14_002856"
     CDS             387..1082
                     /locus_tag="J8V14_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA0922913.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          572 a          217 c          302 g          421 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt ta
//
LOCUS       DADQRX010000213         1482 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.222, whole genome shotgun sequence.
ACCESSION   DADQRX010000213 DADQRX010000000
VERSION     DADQRX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.222"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="J8V14_002857"
     CDS             <1..828
                     /locus_tag="J8V14_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBA0922914.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="J8V14_002858"
     CDS             828..1448
                     /locus_tag="J8V14_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922915.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DADQRX010000214         1479 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.223, whole genome shotgun sequence.
ACCESSION   DADQRX010000214 DADQRX010000000
VERSION     DADQRX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.223"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="J8V14_002859"
     CDS             <1..828
                     /locus_tag="J8V14_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBA0922916.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="J8V14_002860"
     CDS             828..1445
                     /locus_tag="J8V14_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922917.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          544 a          192 c          311 g          432 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcg
//
LOCUS       DADQRX010000215         1418 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.224, whole genome shotgun sequence.
ACCESSION   DADQRX010000215 DADQRX010000000
VERSION     DADQRX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.224"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="J8V14_002861"
     CDS             complement(150..1337)
                     /locus_tag="J8V14_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBA0922918.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DADQRX010000216         1359 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.225, whole genome shotgun sequence.
ACCESSION   DADQRX010000216 DADQRX010000000
VERSION     DADQRX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.225"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..208)
                     /locus_tag="J8V14_002862"
     CDS             complement(<1..208)
                     /locus_tag="J8V14_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922919.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="J8V14_002863"
     CDS             complement(305..757)
                     /locus_tag="J8V14_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922920.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..1312)
                     /locus_tag="J8V14_002864"
     CDS             complement(764..1312)
                     /locus_tag="J8V14_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBA0922921.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          487 a          264 c          132 g          476 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa aataagttac tttttaaata tcttgttta
//
LOCUS       DADQRX010000217         1358 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.226, whole genome shotgun sequence.
ACCESSION   DADQRX010000217 DADQRX010000000
VERSION     DADQRX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.226"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..1324
                     /locus_tag="J8V14_002865"
     CDS             23..1324
                     /locus_tag="J8V14_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBA0922922.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          440 a          271 c          252 g          395 t
ORIGIN      
        1 cacaatcaaa ggagcgttct gtatgcctga tcttcatcat atcaaactac tacttggaat
       61 caaagataaa aacattttca tcacaaatgt ggaatcgaag agcattaaga atatcaagag
      121 tcttgttgtt tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt gtaaacagat
      181 gaatcatgaa ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc aacttaataa
      241 atgtgcgaac caactgactt atcttgcttt agcgaaacaa cgctaccact gtcgtggctg
      301 ccacacctat tttacggcga atacctacat tgtcgataga aactgcttta tcgctaaaca
      361 agtgcgctat aaaattttag aagagctaac tgaaaaacaa gcaatgacga caattgctaa
      421 gcattgcggc gtttcttggt cgaccgtttc tcggacactc gcttctctga tccccatgac
      481 aaaagtaaaa agaaattggt taccgcgttg tttattaatg gatgaatttc gttcactgaa
      541 aaatcaagtc ggtccctact ccttttcttg tatggatggc gatactggaa aattactgga
      601 tattcttcct tcacggaaga aaaaggctct cgtttcttat tttatgcaat ttgaacgacg
      661 cgcacgattg aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg cctctctgat
      721 aaaggaatgt tttccaaagg caaaactcgt tgtggatcgt tttcacattg tcaaacatct
      781 cattcgtaaa tttgaggata ttcgcgtacg aatcatgaaa agctttgatc gaaacgatcc
      841 aacacaagcc aaatattatc gacaattaaa ggcgttgtct cggcttctga tcaaacgaca
      901 ggacaagctt gtttatgaca aatggaccaa atggcgcaat tttggttggg cgtatctcac
      961 cgaaagtgaa gtggtggatc gtctgctttc gatctctgac gaattacgaa tcgcctattt
     1021 ttactatcaa gaaattcttc aagcgtttca cgacaaagag gcggacactt tctttaaact
     1081 tgtaagaacg atgcccaatt ccgttccaaa agaactgcat tctataaaaa aagcttttat
     1141 aaactatgaa tccggcattc gtttagccct cgaattgcct tattcaaatg ccaaaataga
     1201 aaatctgcat acccacatca aagcattgaa acgagtcgct tatggctttc gaagctttcg
     1261 gaaaatgaag accagaattt tcttactaaa taatctgatt acctatgaat caaaaaacat
     1321 ctaaagtaga aaaggaattc taccttagat gttcttat
//
LOCUS       DADQRX010000218         1358 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.227, whole genome shotgun sequence.
ACCESSION   DADQRX010000218 DADQRX010000000
VERSION     DADQRX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.227"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..1282)
                     /gene="tnpB"
                     /locus_tag="J8V14_002866"
     CDS             complement(134..1282)
                     /gene="tnpB"
                     /locus_tag="J8V14_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HBA0922923.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          389 a          240 c          217 g          512 t
ORIGIN      
        1 tgacggttct taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta
       61 tttctttact aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc
      121 ttgatgattg gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat
      181 tgatactagc attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc
      241 gaatttcaag agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg
      301 gaaaccattt atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt
      361 tagtcacaaa ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca
      421 acataccttt tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg
      481 tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta
      541 ctttgcgttt ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag
      601 ccaataaagc acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg
      661 acgtgaattt attattatca attttttgtc catcagaaag aatcgcaaag tccataatac
      721 ctaagtcaat accaattgtg gaattagttt taggtaattc gataatttct tctttacaca
      781 acaaagaaat atagtattta ccgctagaat gacgtgatat tgtagcggat ttgataatcc
      841 cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga
      901 atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag
      961 gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt
     1021 atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa
     1081 acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat
     1141 ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga
     1201 cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt
     1261 tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga
     1321 ttttggatat acttcttaat cacatcaatt ggtgcgcc
//
LOCUS       DADQRX010000219         1356 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.228, whole genome shotgun sequence.
ACCESSION   DADQRX010000219 DADQRX010000000
VERSION     DADQRX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.228"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1356
                     /locus_tag="J8V14_002867"
     CDS             82..>1356
                     /locus_tag="J8V14_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBA0922924.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          461 a          261 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaat
//
LOCUS       DADQRX010000220         1342 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.229, whole genome shotgun sequence.
ACCESSION   DADQRX010000220 DADQRX010000000
VERSION     DADQRX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.229"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="J8V14_002868"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="J8V14_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBA0922925.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="J8V14_002869"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="J8V14_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
BASE COUNT          514 a          222 c          219 g          387 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac ataaaagccc tt
//
LOCUS       DADQRX010000221         1292 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.230, whole genome shotgun sequence.
ACCESSION   DADQRX010000221 DADQRX010000000
VERSION     DADQRX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.230"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..280)
                     /locus_tag="J8V14_002870"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="J8V14_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="J8V14_002871"
     CDS             complement(546..740)
                     /locus_tag="J8V14_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922926.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          458 a          217 c          175 g          442 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa ta
//
LOCUS       DADQRX010000222         1276 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.231, whole genome shotgun sequence.
ACCESSION   DADQRX010000222 DADQRX010000000
VERSION     DADQRX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.231"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="J8V14_002872"
     CDS             complement(31..1203)
                     /locus_tag="J8V14_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HBA0922927.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          421 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttat
//
LOCUS       DADQRX010000223         1272 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.232, whole genome shotgun sequence.
ACCESSION   DADQRX010000223 DADQRX010000000
VERSION     DADQRX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.232"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..695)
                     /locus_tag="J8V14_002873"
     CDS             complement(27..695)
                     /locus_tag="J8V14_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBA0922928.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            829..1116
                     /locus_tag="J8V14_002874"
     CDS             829..1116
                     /locus_tag="J8V14_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922929.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          475 a          214 c          214 g          369 t
ORIGIN      
        1 ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg atcaagtggt
       61 tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga
      121 tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa
      181 tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga
      241 cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg
      301 cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc
      361 atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa
      421 tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca
      481 aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga
      541 gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc
      601 ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt
      661 ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat
      721 tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa
      781 aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca
      841 gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg
      901 gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt
      961 atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg
     1021 atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat
     1081 ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt
     1141 tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata
     1201 atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct
     1261 gtttcttttt at
//
LOCUS       DADQRX010000224         1252 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.233, whole genome shotgun sequence.
ACCESSION   DADQRX010000224 DADQRX010000000
VERSION     DADQRX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.233"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..>1252
                     /locus_tag="J8V14_002875"
     rRNA            20..>1252
                     /locus_tag="J8V14_002875"
                     /product="23S ribosomal RNA"
BASE COUNT          383 a          248 c          360 g          261 t
ORIGIN      
        1 atcaaaaccc aaccgtaagg ttgataaggt taagtgaata agggcgcacg gtggatgcct
       61 tggcactagg agccgatgaa ggacgggact aacaccgata tgctttgggg agctgtacgt
      121 aagctatgat ccagagattt ccgaatgggg gaacccaaca tctttaatag gatgttacga
      181 ttgtgtgaat acatagcaca ttcgaggtag acgcagagaa ctgaaacatc taagtacctg
      241 caggaagaga aagaaaattc gattccctga gtagcggcga gcgaaacggg aaaagcccaa
      301 accagcaagc ttgcttgttg gggttgtagg actccaatat ggtagttctt tcagatagtc
      361 gaatgacttg gaaaagtcag tcaaagaggg taaaaacccc gtagacgaaa tgtggaagac
      421 acctaggagg atcctgagta cggcggaaca cgagaaattc cgtcggaatc cgggaggacc
      481 atctcccaag gctaaatact ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg
      541 tgaaaagcac cccggaaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtc
      601 aaagcccgtt aatgggtgat ggcgtgcctt ttgtagaatg aaccggcgag ttacgattgc
      661 atgcgaggtt aagttgaaga gacggagccg cagcgaaagc gagtctgaat agggcgtttg
      721 agtatgtagt cgtagacccg aaaccatgtg atctacccat gtccaggttg aaggtgcggt
      781 aaaacgcact ggaggaccga acccacgtac gttgaaaagt gcggggatga ggtgtgggta
      841 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
      901 agcctcggaa ttgagaatga tggaggtaga gcactgtttg gactaggggc ccatctcggg
      961 ttaccgaatt cagataaact ccgaatgcca ttcattcata tccgggagtc agactgtgag
     1021 tgataagatc catagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaatat
     1081 atgttaagtg gaaaaggatg tggggttgca cagacaacta ggatgttggc ttagaagcag
     1141 ccaccattta aagagtgcgt aatagctcac tagtcgagtg accctgcgcc gaaaatgtac
     1201 cggggctaaa catattaccg aagctgtgga gtacaccttt aggtgtattg gt
//
LOCUS       DADQRX010000225         1251 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.234, whole genome shotgun sequence.
ACCESSION   DADQRX010000225 DADQRX010000000
VERSION     DADQRX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.234"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            285..1100
                     /locus_tag="J8V14_002876"
     CDS             285..1100
                     /locus_tag="J8V14_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922930.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          481 a          172 c          202 g          396 t
ORIGIN      
        1 ataagaaatt caaaaaagaa aaaactttcc ttaaaaacaa aaaataggag tattcagttt
       61 cttaatagtt ttgtaatagt tgtatgtaga agttttctta atagtttttg taataattta
      121 ttaatcgtag tttatataac ttatttgtaa gtgactagag aattaaataa aaaataatta
      181 ctatctagta cgaaattaat tacataggat aatatattaa ggtgctggac tacaagtaaa
      241 aaatactgta tgatcatgac agattcaaga aaggagcaat aagattggtt aagatagcag
      301 gactttctaa aggtggaaaa accgtttcac aactttataa acaaaactac agagactgga
      361 gacagttcag aaaaaacatt aaggattctt actttattct accaacagaa cttgaaaaat
      421 atttaccgaa taacaaagct attaatttat atctgtatta ctgttttaag gcaaagaatg
      481 aaggcgggga ttcctggcat tcggtcaata ccatagcaga gtctctagga gtgacaacta
      541 aaactataaa cacctggaat aaaaccttag ttgatttagg tatgattgca cgtatagacg
      601 ataaccatct cagtaaatcg acaattttac ttcctctctc tagttactat aaattagccc
      661 ttgatagttc tttaaaggat gttaccgaga gcactgtcca tgaaattgat ggcaacttgg
      721 ttgcagttta tcatttgttt gaatggagaa aaaatgaggc tgatgaaaat tataacgttc
      781 cttataacac actctgttgt gtttttaaaa gaaaaacaca aaaagatact atctataaat
      841 ttatcttggt ccaagatgag aaaataagta atttcgaatt aagcactcct tctagtaaga
      901 tttatgacga tgcatataga tttgaatgtc aagatctaga agccctcctt tccgcaaata
      961 atctaaacaa tatagaacat gaaaactttg ttgtaaacac tagatttaat ttagtttctg
     1021 aaaaggactc agatttgtta gatttgctga tagagcttac taaaaatcta gataagaaag
     1081 aaaacattac tgtactataa ctagcatcgg tccatcatta agtttggact attagaacac
     1141 ttgctattac agaaaaaggt atgctagcca cgcttactat ttattggtat caaacaagta
     1201 tcttaatttg aaaggaagat gatttatggc taatttatat atagagaaca a
//
LOCUS       DADQRX010000226         1215 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.235, whole genome shotgun sequence.
ACCESSION   DADQRX010000226 DADQRX010000000
VERSION     DADQRX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.235"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="J8V14_002877"
     CDS             414..947
                     /locus_tag="J8V14_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA0922931.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DADQRX010000227         1213 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.236, whole genome shotgun sequence.
ACCESSION   DADQRX010000227 DADQRX010000000
VERSION     DADQRX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.236"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..719
                     /locus_tag="J8V14_002878"
     CDS             48..719
                     /locus_tag="J8V14_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA0922932.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          445 a          185 c          170 g          413 t
ORIGIN      
        1 aacagaatta aaatatcagt taaaataaaa gttaaggagc attgtgtatg acaattaaaa
       61 ctagtctcca aaatcaagct taccaatcta ttcgccgaca aattatctat gctgacttag
      121 aacctggaaa aaaagtatca gaaaaaaaat tagaagaaac actttctatt ggtcgtacac
      181 caatacgtga agcactgatt caactccgac aacaaggtct tgttgataca attcctcaaa
      241 gcggaaatta tatctcatta attgatttag attcggccaa aaatgctcga tttgtcagag
      301 aacatttgga acgacaaatt atgttagagt gttgtgccaa aatgaataac caaaaaaaac
      361 ggattttaga aactataatt tcagaacaag aaaaagctgt tagtcaaaaa aatgctcgta
      421 gtttttttca taatgataat ttattccata aagcttgttt tgaaattgca gagcgagaag
      481 aagtatggca atggttagaa aataacaata cacacttaga acgatttcga tggctacgag
      541 taaaaacgga aggattaaag tgggaaaaaa ttatggaaca acattatcaa ctatttgagg
      601 cattaacaaa acaagatcca gaagaagcta actttctaac atcgttgcat cttcatttaa
      661 tgcttaatga gcaaaatatt gttttagcta ctcatccaaa atatttcaaa aatatataac
      721 ttttaactct ttttaaatta aatggttgtt ctgatcagaa aaatatcgct tgttttctta
      781 ttatgtaata tatttcataa actattctgc ataacaacta ttgtttgtac tttttacatt
      841 ggataactaa ttatgactgc tgataacttt tgatattaaa ctagtatctc caatacaaat
      901 aaaaaataga tatcttgttc atcaaaatta gttcgtggat acttaccttc atttacatca
      961 ttcagcggtt tcccattttc ttttattcta ttatttcatt ttctaataga cttggcttca
     1021 tttcgcaata ctttttttca ataggcatca aattggtgtt gtttagatat ttagttgtag
     1081 ttttgtgaca tgataagtat gctccttttt ctcttttcca ctaatacaac gatcaaaggg
     1141 tgcttttttc aaaaaactag aaaaatcaaa ataagattcg tattttgatt ctatatatcc
     1201 tgtttcaact aca
//
LOCUS       DADQRX010000228         1198 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.237, whole genome shotgun sequence.
ACCESSION   DADQRX010000228 DADQRX010000000
VERSION     DADQRX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.237"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            56..316
                     /locus_tag="J8V14_002879"
     CDS             56..316
                     /locus_tag="J8V14_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922933.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(678..917)
                     /locus_tag="J8V14_002880"
     CDS             complement(678..917)
                     /locus_tag="J8V14_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922934.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          425 a          165 c          220 g          388 t
ORIGIN      
        1 attttaatgg tattatcatt ttaaataatg gaaaaacatt aaggatggat gaaatatgaa
       61 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
      121 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
      181 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
      241 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
      301 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
      361 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
      421 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
      481 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
      541 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
      601 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
      661 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
      721 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
      781 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
      841 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
      901 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
      961 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
     1021 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
     1081 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
     1141 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggata
//
LOCUS       DADQRX010000229         1193 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.238, whole genome shotgun sequence.
ACCESSION   DADQRX010000229 DADQRX010000000
VERSION     DADQRX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.238"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="J8V14_002881"
     CDS             <1..1076
                     /locus_tag="J8V14_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA0922935.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DADQRX010000230         1170 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.239, whole genome shotgun sequence.
ACCESSION   DADQRX010000230 DADQRX010000000
VERSION     DADQRX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.239"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..963
                     /locus_tag="J8V14_002882"
     CDS             4..963
                     /locus_tag="J8V14_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0922936.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          393 a          219 c          234 g          324 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg
//
LOCUS       DADQRX010000231         1166 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.240, whole genome shotgun sequence.
ACCESSION   DADQRX010000231 DADQRX010000000
VERSION     DADQRX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.240"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1166)
                     /locus_tag="J8V14_002883"
     CDS             complement(<1..>1166)
                     /locus_tag="J8V14_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBA0922937.1"
                     /translation="KGRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELK
                     RTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTV
                     TRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRL
                     YKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFN
                     QLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEI
                     VDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRY
                     QSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          328 a          228 c          253 g          357 t
ORIGIN      
        1 agttttcaaa gatcagacct tgttgtaaat agatccgtgc ccgaaagtta taaaagttgc
       61 gataaccaaa cgcaattcgt ttaatcagct tgatcttgtt attcagcccc tcagtgacgc
      121 cgtttgagtg gggcacctta aaggcatttg aaatacccga ttggtagcga tggaagatcg
      181 tgaatttctt tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa
      241 agccttccgt atctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag
      301 ttcgcaattc ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc
      361 gtttaaacag tcgatgataa tgccgtttga gtggctccaa tgtcgtggaa tctttgagta
      421 agagtttcca atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc
      481 gaaaacgatt catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa
      541 tatgaaagcg gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat
      601 agctggcatt catgtccatc actaggtact tgacctttaa acgggccttt ctaggaaagc
      661 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
      721 tggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      781 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      841 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      901 tacgtgattg attttcgtac agttccatga gaatcgcctg cttcagttcc tttgttagtt
      961 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccgtccacaa tcatggcaga
     1021 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aacatctgag
     1081 tgcgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
     1141 aaggtgagtc atccaatctc ccttta
//
LOCUS       DADQRX010000232         1161 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.241, whole genome shotgun sequence.
ACCESSION   DADQRX010000232 DADQRX010000000
VERSION     DADQRX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.241"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="J8V14_002884"
     CDS             <1..969
                     /locus_tag="J8V14_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0922938.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          204 c          232 g          331 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt a
//
LOCUS       DADQRX010000233         1136 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.243, whole genome shotgun sequence.
ACCESSION   DADQRX010000233 DADQRX010000000
VERSION     DADQRX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.243"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1061)
                     /locus_tag="J8V14_002885"
     CDS             complement(<1..1061)
                     /locus_tag="J8V14_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922939.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          301 a          254 c          182 g          399 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaac
//
LOCUS       DADQRX010000234         1112 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.244, whole genome shotgun sequence.
ACCESSION   DADQRX010000234 DADQRX010000000
VERSION     DADQRX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.244"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..417
                     /locus_tag="J8V14_002886"
     CDS             154..417
                     /locus_tag="J8V14_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBA0922940.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="J8V14_002887"
     CDS             417..689
                     /locus_tag="J8V14_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBA0922941.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="J8V14_002888"
     CDS             766..1095
                     /locus_tag="J8V14_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA0922942.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          423 a          141 c          220 g          328 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa ac
//
LOCUS       DADQRX010000235         1103 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.245, whole genome shotgun sequence.
ACCESSION   DADQRX010000235 DADQRX010000000
VERSION     DADQRX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.245"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..153)
                     /locus_tag="J8V14_002889"
     tRNA            complement(69..153)
                     /locus_tag="J8V14_002889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Leu,seq:caa)
     gene            complement(174..244)
                     /locus_tag="J8V14_002890"
     tRNA            complement(174..244)
                     /locus_tag="J8V14_002890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Cys,seq:gca)
     gene            complement(290..361)
                     /locus_tag="J8V14_002891"
     tRNA            complement(290..361)
                     /locus_tag="J8V14_002891"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Gln,seq:ttg)
     gene            complement(373..445)
                     /locus_tag="J8V14_002892"
     tRNA            complement(373..445)
                     /locus_tag="J8V14_002892"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:His,seq:gtg)
     gene            complement(448..521)
                     /locus_tag="J8V14_002893"
     tRNA            complement(448..521)
                     /locus_tag="J8V14_002893"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Trp,seq:cca)
     gene            complement(528..608)
                     /locus_tag="J8V14_002894"
     tRNA            complement(528..608)
                     /locus_tag="J8V14_002894"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Tyr,seq:gta)
     gene            complement(610..685)
                     /locus_tag="J8V14_002895"
     tRNA            complement(610..685)
                     /locus_tag="J8V14_002895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Phe,seq:gaa)
     gene            complement(721..793)
                     /locus_tag="J8V14_002896"
     tRNA            complement(721..793)
                     /locus_tag="J8V14_002896"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(758..760),aa:Asp,seq:gtc)
     gene            complement(803..875)
                     /locus_tag="J8V14_002897"
     tRNA            complement(803..875)
                     /locus_tag="J8V14_002897"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Val,seq:tac)
     gene            complement(891..962)
                     /locus_tag="J8V14_002898"
     tRNA            complement(891..962)
                     /locus_tag="J8V14_002898"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(927..929),aa:Glu,seq:ttc)
     gene            complement(977..1066)
                     /locus_tag="J8V14_002899"
     tRNA            complement(977..1066)
                     /locus_tag="J8V14_002899"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1030..1032),aa:Ser,seq:gga)
BASE COUNT          311 a          288 c          250 g          254 t
ORIGIN      
        1 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
       61 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
      121 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
      181 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
      241 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
      301 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
      361 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
      421 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
      481 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
      541 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
      601 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
      661 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
      721 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
      781 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
      841 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
      901 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
      961 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
     1021 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
     1081 tcaatactct ttctcaaaat gag
//
LOCUS       DADQRX010000236         1101 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.246, whole genome shotgun sequence.
ACCESSION   DADQRX010000236 DADQRX010000000
VERSION     DADQRX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.246"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            70..1017
                     /locus_tag="J8V14_002900"
     CDS             70..1017
                     /locus_tag="J8V14_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBA0922943.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          352 a          170 c          234 g          345 t
ORIGIN      
        1 ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat ttatatcgga
       61 ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg ttctacctta
      121 gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc aatgggaaaa
      181 gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt cgctgagaaa
      241 tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca tttagcagca
      301 gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt gaattttgaa
      361 agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa acggatcgtg
      421 tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa agaagagtcc
      481 gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca atatgtgcta
      541 gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa tgtctatggt
      601 ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt agatcgctat
      661 aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg aagccagtct
      721 agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc gaatgaggaa
      781 gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact tttggaattg
      841 attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa agaagagcgt
      901 tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat tggctatcaa
      961 ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat caattaaaac
     1021 atactaagat aagattagag ggtaaattca ggctctttgt caataaggac tgatgagttt
     1081 tgtaaaatca aatctgggat a
//
LOCUS       DADQRX010000237         1083 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.247, whole genome shotgun sequence.
ACCESSION   DADQRX010000237 DADQRX010000000
VERSION     DADQRX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.247"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..206)
                     /locus_tag="J8V14_002901"
     tRNA            complement(133..206)
                     /locus_tag="J8V14_002901"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Ile,seq:cat)
     gene            complement(761..1075)
                     /locus_tag="J8V14_002902"
     CDS             complement(761..1075)
                     /locus_tag="J8V14_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922944.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          405 a          172 c          166 g          340 t
ORIGIN      
        1 aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt gagtggcaat
       61 gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga acgattcaat
      121 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
      181 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      241 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      301 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      361 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      421 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      481 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      541 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      601 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      661 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      721 gtaagaagta ataattcctg tactaaaagg cataattgct ttaattattt tttgacttct
      781 cttcaaacac aagaagtagt tgtgtaataa ttgcagaaat aaggattcca ataatagata
      841 taaaatttga catggaaacg tctataacat taaaagaata aagtgttgaa gaaagtaaac
      901 tgattgaaat aacccctccg taaaaaatag tctgtgaata tacccacttt ttttcagaag
      961 acgtacttcg tttggttcta aaacctatcc ctgcatttct actcttatca aaaaaccaaa
     1021 tatttagaag taataatatt gctattatta agataaaatt actgtaaaaa tccaaaagct
     1081 atc
//
LOCUS       DADQRX010000238         1080 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.248, whole genome shotgun sequence.
ACCESSION   DADQRX010000238 DADQRX010000000
VERSION     DADQRX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.248"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(193..423)
                     /locus_tag="J8V14_002903"
     CDS             complement(193..423)
                     /locus_tag="J8V14_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922945.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(673..873)
                     /locus_tag="J8V14_002904"
     CDS             complement(673..873)
                     /locus_tag="J8V14_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA0922946.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          383 a          173 c          157 g          367 t
ORIGIN      
        1 ctttcttcat tctagttttc gcagcaggga ttttaaagta gttcttatta gaatagtaga
       61 gattaattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      121 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgc gtctcattaa
      181 aaagcataac cattactccg atctattttt aaacttagtc tctacctcta atatatatcg
      241 agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag gttgtttatt
      301 ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttgt ctttttcgtt
      361 gctttgctca taaaaatctg catattcatc actaatattt tttctaataa attcttccat
      421 cataaagatc acctcgaaaa gagtatacga acaaacgttc tttttgtaaa ggtggaattt
      481 attatactaa ataaataggt gaacaagtac ttgtgccaaa ttatgtgcca aaaaaaatcg
      541 aatttaataa aacacaaaca aaaagaaatc ctattatgat aacatttctt ataataacaa
      601 acacaacaga agacgtgtaa tagttagtca ggaacgtaca aataacccct gtatcctttg
      661 cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc tatgtcctaa
      721 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgcgtatg
      781 tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta ctaaagaaaa
      841 acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa ttaaacgatt
      901 tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac tctagcggta
      961 taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt ttattatttt
     1021 tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca tataaaaaaa
//
LOCUS       DADQRX010000239         1063 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.249, whole genome shotgun sequence.
ACCESSION   DADQRX010000239 DADQRX010000000
VERSION     DADQRX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.249"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..994)
                     /locus_tag="J8V14_002905"
     CDS             complement(38..994)
                     /locus_tag="J8V14_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922947.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DADQRX010000240         1049 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.250, whole genome shotgun sequence.
ACCESSION   DADQRX010000240 DADQRX010000000
VERSION     DADQRX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.250"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1049
                     /locus_tag="J8V14_002906"
     CDS             <1..>1049
                     /locus_tag="J8V14_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBA0922948.1"
                     /translation="ICLIYKGTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSE
                     YRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNA"
BASE COUNT          307 a          209 c          217 g          316 t
ORIGIN      
        1 atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc ccattgtctt
       61 tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt gttactgaat
      121 gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa atgtaagtct
      181 tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt cattagtcaa
      241 aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac ggaaatcgct
      301 cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca
      361 ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt caaatcggtt
      421 cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca tgcattattg
      481 gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg gttttcatta
      541 gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta tgtttcgttg
      601 gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat cgtccaacat
      661 attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact taaaggaaat
      721 tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact cctacaaaaa
      781 gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa agattatttg
      841 actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg ccaaggctat
      901 cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact atttgaggat
      961 tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt acgaacgttt
     1021 aaaaagtttc tgccacagat ccaaaatgc
//
LOCUS       DADQRX010000241         1032 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.251, whole genome shotgun sequence.
ACCESSION   DADQRX010000241 DADQRX010000000
VERSION     DADQRX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.251"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..770
                     /locus_tag="J8V14_002907"
     CDS             78..770
                     /locus_tag="J8V14_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBA0922949.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          448 a          152 c          174 g          258 t
ORIGIN      
        1 atttaaaaac gcttgacatt gtgtttgaga tcacttactt ttagcatgta aaagtattgg
       61 attaaggagc tatgcaaatg aaaaagaaat ttattactgg tgggattgtc gttgcatttc
      121 tatgtctaat tggctacaca ggcacgcggt acgcaaatga aattattttg tggggtggag
      181 agacagatat tgatacaatc aatgagaatc tgacacagct tgatcataca ttgacagata
      241 aagagcaagc gattacagat cttaccgctc gactagcaca aaaagaacag acattgcaag
      301 attcaaaggc agatgtccaa gaataccaaa caaaagtaaa tgaattagaa aaccaaaaaa
      361 atcaatcggc agcagaaaaa caaaacttag aaagtcaatt agtcagcaag gatgcacaac
      421 tacaaagcaa acaaaatgaa atcaatgtca aactagatga gatcaaccaa aaaaatgatg
      481 aaatcaatca tctgaatgca gattggacag caagactaca agaagcacaa gcaaaacttt
      541 cagaattaca aaatactgta aatggtttga atcaacaaat cgcaacactg acacaagaaa
      601 aaacaaatgt agaaactcag ttaacagata cacaaaagaa attagacgaa gcaactgctg
      661 aaaactcaag cttgaaacaa tatattcaaa agttagaaaa agcaaaagat gaagtggaag
      721 atacagctga taaatctcag caaattgttg aagaacattt gcgtaaataa tctttcttaa
      781 caagtaaacc taaattagta aagaagcaac agtaatctat actaagtagt tcagttttgg
      841 agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta acttacatac
      901 caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga atgaaaattt
      961 gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt caactaacgg
     1021 gcaggataat ga
//
LOCUS       DADQRX010000242         1001 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.252, whole genome shotgun sequence.
ACCESSION   DADQRX010000242 DADQRX010000000
VERSION     DADQRX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.252"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..1001
                     /locus_tag="J8V14_002908"
                     /pseudo
     CDS             369..1001
                     /locus_tag="J8V14_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DADQRX010000243          995 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.253, whole genome shotgun sequence.
ACCESSION   DADQRX010000243 DADQRX010000000
VERSION     DADQRX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.253"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..991
                     /locus_tag="J8V14_002909"
                     /pseudo
     CDS             1..991
                     /locus_tag="J8V14_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          353 a          176 c          197 g          269 t
ORIGIN      
        1 ataatcttac gcctgaacaa aaagaattgt tagaactacg taaaagaaac cagcaattag
       61 aaatggaaaa tgatatttta aagcaagcag cgctgatatt cggacgaaga gacaagtaat
      121 cgatgcgaat aagcatcttt accctatatc agcgatgtgc agaatattag gtctatcacg
      181 tcagtcctat tattatcaat caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt
      241 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
      301 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      361 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      421 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      481 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      541 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataaagatg ccgttctagt
      601 aaaaaaagca tttagccgta tcaaacaacc tctgacagag gttgagattt ttcatactga
      661 tcgtggaaaa gagtttgata accaagctat tgatgaatta ttaacaactt ttgacatcaa
      721 tcgatcattg agtcataaag gctgtccttt tgataatgcc gtagctgaat caacttataa
      781 gtcgttgaaa gtagaatttg tctatcaata cacatttgaa accttacaac aattggattt
      841 ggagttattt gactatgtca attggtggaa ccaccttcgg ttgcacggta cacttggcta
      901 cgagacaccg gttggttacc gtaaccagag attggcgcag cgaatccttg ataatgagct
      961 cggatgtgct aacgctagcg aggcagtcta acttt
//
LOCUS       DADQRX010000244          992 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.254, whole genome shotgun sequence.
ACCESSION   DADQRX010000244 DADQRX010000000
VERSION     DADQRX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.254"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..>992
                     /locus_tag="J8V14_002910"
     CDS             51..>992
                     /locus_tag="J8V14_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922950.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDD"
BASE COUNT          356 a          180 c          189 g          267 t
ORIGIN      
        1 tgctacactc aaaatagttc cgacgaaaga attataagga gtgagtacaa atgacctata
       61 accatcttac accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacaacctg
      121 tttcaaaagt agccaatctg ttacaacgtt ctcgacaaac tatttataag gtctatcgtt
      181 ttcttaaatc tggtggaagc gcaatgagct actataaaca atataagaaa aacaaacgta
      241 actgcggaag acatccgatt gttttacctg atgagcaaca ggaatacatt caaaaaaagg
      301 ttgttcaagg gtgggctcct gatgtcctta ttggtcgagc agagtttcct attgcttgtt
      361 cggtacgcac actgtatcga atgtttaaag ataaaacctt tgacactcac gatttaccaa
      421 tgaagggaaa aagaaaacca aacggtcata aagaaaaaag aggaaaacaa gggtttcgcc
      481 ggtcaatccg agacagaaag acagactata cccagtttga tcatgaattt ggtcacctgg
      541 aaggtgacac tatcgtgggt ggaaaacata aaagcgcggt catcacatta gttgaacggc
      601 tatctaaagt catcattacc ttgaaacccg aaggcagaca ggctaaagac attgaaaata
      661 ggctgaatca atggtttcgt tgtgtcccaa aaaacttatt taaatccatc acatttgatt
      721 gtggaaaaga attctcaaat tggaaaaaga ttagtaatac caacgatatt tcaatttatt
      781 ttgcggatcc aggaacacct tctcaacgag gattgaatga acattcaaat ggcttattac
      841 gtaaagatgg tttgtataaa gctatggatt ttaatggtgt atctgaaacc tttatccaat
      901 cggtggcatc gaaacgaaat catattccta gaaaatcatt acactatcgg acaccaatgg
      961 aagtattttt gagttacgtc acaaaagatg at
//
LOCUS       DADQRX010000245          961 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.255, whole genome shotgun sequence.
ACCESSION   DADQRX010000245 DADQRX010000000
VERSION     DADQRX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.255"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="J8V14_002911"
                     /pseudo
     CDS             172..459
                     /locus_tag="J8V14_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="J8V14_002912"
     CDS             473..904
                     /locus_tag="J8V14_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922951.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DADQRX010000246          935 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.256, whole genome shotgun sequence.
ACCESSION   DADQRX010000246 DADQRX010000000
VERSION     DADQRX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.256"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..230
                     /locus_tag="J8V14_002913"
     CDS             <1..230
                     /locus_tag="J8V14_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBA0922952.1"
                     /translation="EKPDLVICTGVLAMIPICLITKMMGKKLIYIESFAKVTSPTQTG
                     KLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            247..726
                     /locus_tag="J8V14_002914"
     CDS             247..726
                     /locus_tag="J8V14_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBA0922953.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
BASE COUNT          353 a          105 c          182 g          295 t
ORIGIN      
        1 aggaaaaacc ggatctcgtg atttgtacag gtgttttagc tatgatccct atctgcttaa
       61 taacaaaaat gatgggaaaa aaactgattt atattgaatc gtttgcaaaa gtaaccagcc
      121 ctactcagac aggaaaattg atgtatcatt ttgcagatca attttatgtt cagtgggaaa
      181 ctatgctaca attttatcct aatgctattt atttgggtgg gatatattaa ataataggag
      241 aaaaaaatga tatttataac attaggttca caaaaatttc aatttgatag attgttaaaa
      301 gctattgatg atttaattga aaaaggagta ataatcgaaa gtacatttgc acagagtggt
      361 tatagtgatt ataaaccact aaattattcc tataaagcat ttctaaatag ggatgagttt
      421 actgaaacta tgcaaaaagc agaaatggtg attactcatg gaggtacagg tgctataatt
      481 ggggccgtaa aaaaaggtaa aagggtcatt gctgttcctc gattggctaa gtatggtgag
      541 catgtcgatg atcaccaact gcagttaatt agacaatttg atgagatgaa tattatctat
      601 ccttgttatg aattagaaaa tttagaagat gccgttaaag aaacaagagt tcggatgttt
      661 aacaagtatg tatctaacac ggaaacgatt attaattcga tagaggtatt aatagaaaaa
      721 atataaatat aaggagtaaa gcgttatgca aagagtactt atgattggta atgacagctc
      781 ggtaaaaggt ggaattacaa gtataattac acaaatgctc aattttaatt gggaaaaaaa
      841 gaatatcgag atgactttta taccgagtta taagggtgga aatgcaatat ttaaatcttt
      901 atattttatg tatgcatttg ctaaaattat aggta
//
LOCUS       DADQRX010000247          910 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.257, whole genome shotgun sequence.
ACCESSION   DADQRX010000247 DADQRX010000000
VERSION     DADQRX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.257"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="J8V14_002915"
     CDS             <1..355
                     /locus_tag="J8V14_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922954.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="J8V14_002916"
                     /pseudo
     CDS             615..910
                     /locus_tag="J8V14_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DADQRX010000248          894 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.258, whole genome shotgun sequence.
ACCESSION   DADQRX010000248 DADQRX010000000
VERSION     DADQRX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.258"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            253..876
                     /locus_tag="J8V14_002917"
     CDS             253..876
                     /locus_tag="J8V14_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922955.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaag
//
LOCUS       DADQRX010000249          880 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.259, whole genome shotgun sequence.
ACCESSION   DADQRX010000249 DADQRX010000000
VERSION     DADQRX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.259"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            36..266
                     /locus_tag="J8V14_002918"
     CDS             36..266
                     /locus_tag="J8V14_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922956.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(470..835)
                     /locus_tag="J8V14_002919"
     CDS             complement(470..835)
                     /locus_tag="J8V14_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922957.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          346 a          105 c          158 g          271 t
ORIGIN      
        1 atatatggat ttttgatagg agtagaaatg caagtatagg ttttagaact aaacgaagtc
       61 tgtcttctaa aaaaaattgg gtatactcac aaactatttt ttatggagga attgttttaa
      121 ttagtttact ttcttcaaca ctttattctt tgaatattat agatgtttcc acctcaaatt
      181 caatatctat tattggaatc attattgctg caattattac acaactgttt ttagtgtttg
      241 gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga tagtgatatt
      301 aaaaatcaga aaagatagaa aaagcaggag atagataaaa tgctgatatt tcagcatttt
      361 ttaagagtat aaaagagaaa aaaagtaata aatgtataca tgatataaga aaaacatata
      421 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      481 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      541 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      601 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      661 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      721 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      781 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      841 atcaccacct aacaagtgtt attattttgt tagttttatt
//
LOCUS       DADQRX010000250          851 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.260, whole genome shotgun sequence.
ACCESSION   DADQRX010000250 DADQRX010000000
VERSION     DADQRX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.260"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>851
                     /locus_tag="J8V14_002920"
     CDS             <1..>851
                     /locus_tag="J8V14_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBA0922958.1"
                     /translation="KKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISLIYA
                     KSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFK
                     NKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVD
                     SLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENV
                     YIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVK
                     FDLNKEN"
BASE COUNT          379 a           70 c          112 g          290 t
ORIGIN      
        1 gtaaaaaaac aaatctacaa tttgtttcta cagctgatat gcaaaaagat gcgaaagatt
       61 taagccttaa atttttacca tattctttat taatatcaat tttattttct gctattattt
      121 ctttaattta tgcaaaatca ataaaaaata atatacaaga aataaaaatt gttactgata
      181 aaatgatgaa acttgataaa aaaatgagtt taaaagttag ctctaatgat gaagttggag
      241 aattaaaaca acaaattaat gatttgtatt ctactttatt aagaacaatt gacgatttgg
      301 aatttaaaaa taaagaaatt ttaaaattag aaaagttaaa atatgacttt tttaaaggtg
      361 catcccatga acttaaaacc cctcttgcta gtcttaaaat aatactggaa aacatgaaat
      421 acaatattgg aaaatataaa gaaagagata tttatattaa tgaatgtatt gaaatcgttg
      481 atagtttaac aaaaaatatt tctcaaatat tatcagttca ttctatagaa aatttgaata
      541 atgatgaaga atatttgaaa ataaatgata cattagaaga catattaaaa aagtatgaaa
      601 tattagcaaa tcaaaagaaa ataactgtca ataattatat attagacgag aatgtttata
      661 taggaaaaac agctttaaag attattctat ctaatttgat tagtaatgcg gtaaaatata
      721 ctgatgtaaa tggggtaatt aatattggga tagttaatga ttggttatat atagagaatt
      781 catacggtaa caataaaatt tcgaatatgg ataaaatatt tgatgttaaa tttgatttaa
      841 ataaggaaaa t
//
LOCUS       DADQRX010000251          843 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.261, whole genome shotgun sequence.
ACCESSION   DADQRX010000251 DADQRX010000000
VERSION     DADQRX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.261"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..532)
                     /locus_tag="J8V14_002921"
     CDS             complement(20..532)
                     /locus_tag="J8V14_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922959.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(539..>843)
                     /locus_tag="J8V14_002922"
     CDS             complement(539..>843)
                     /locus_tag="J8V14_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922960.1"
                     /translation="FSKNKLKDINSIDNYYIQFKGFDFNSSTEFKLALYAQFFIEKLN
                     RFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          292 a          117 c           77 g          357 t
ORIGIN      
        1 tattttaaaa ctaacatcct taatatctta atttatccat attttgagaa ataatacttt
       61 tccattcaga aactaattta gtaaggaatc ttgcaatgtt ttgtctcatt aattcattat
      121 ttctattatc tataagatct gttagatatt tttcaaattc taaaaagcta tccgaatttt
      181 cttcgacgat taaatactta agagaattta tatcggaaaa gatagattca agtttactaa
      241 tctcattttc taaatctata ttttcacgtg tgattatctc atcacttttt cttaaaaagc
      301 taaaaattgc atcagttgct ttacactttt ccattaaaga agtatattct tcaatttttc
      361 ttctttcttt atggtcattt tttaaatcag tttcaatgtc ttttttcatt tgttctatac
      421 tgagtttgtt ctttttgttc ttttgagtaa atctaaaatt caaacacgaa aaaagtatgt
      481 tagcaataac acctataata gcaaataaat catttatact atttaatttc atttatcatc
      541 acaaacttag aactttactt atcatctcat aagacaagtt tccaaattga taattattgg
      601 tagattttaa atcattataa ttttcgagaa attttatata agtacctctc atcggtttat
      661 ggctattaaa agtgttaaaa tcactatctt caactatttc ttgaaaccta tttaattttt
      721 caataaaaaa ttgtgcataa agtgctaact taaattcggt agagctatta aagtcaaaac
      781 ctttaaattg tatataataa ttatcaattg aatttatatc ttttaattta tttttactaa
      841 aag
//
LOCUS       DADQRX010000252          790 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.262, whole genome shotgun sequence.
ACCESSION   DADQRX010000252 DADQRX010000000
VERSION     DADQRX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.262"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="J8V14_002923"
     CDS             7..624
                     /locus_tag="J8V14_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922961.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          307 a           97 c          146 g          240 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt
//
LOCUS       DADQRX010000253          769 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.263, whole genome shotgun sequence.
ACCESSION   DADQRX010000253 DADQRX010000000
VERSION     DADQRX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.263"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..738
                     /locus_tag="J8V14_002924"
     CDS             <1..738
                     /locus_tag="J8V14_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBA0922962.1"
                     /translation="NPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVA
                     DRFHISQHIGRAFTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPS
                     FRDHLTEAEIVDRLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHY
                     QPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYR
                     GKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          251 a          175 c          149 g          194 t
ORIGIN      
        1 aatcctttac gcgactattt cttgcgctac cctagaaaag tccgtgaaca agtacgtcta
       61 gtggtttcag atttctatac accctatcgc acattggtga aagagtgctt tccgaatgcc
      121 cgaatcgtcg ccgatcgctt tcatattagc caacacatcg gtcgggcctt taccaaccac
      181 cgaattcaag taatgaagac ctttaaaaaa agggatcgtc ggtcgaagca cttaaagaaa
      241 tactggagac tacttcaaaa gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt
      301 ccgtcatttc gagatcatct gacagaagca gagatagtcg atcggctatt atcttatgat
      361 gactccttaa agcgaggcta cgaagtctac caggattttc tttccgctat cagaagacaa
      421 gatgttcctg aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactac
      481 caaccagtgt tcacgacctt caagaaatat cgaacagaaa tcaaacgagc gttgcgagtt
      541 ccctattcca acggccctat agaatgtttg aacaaccata tcaaagtact aaaacgaatt
      601 gcctatggct ttagaaactt tcaaaactac caagaacgaa tctttttata tcgaggaaaa
      661 tattttaaga aaactaagaa cactacccaa ctgactaaag ctagaacaac aacacggcta
      721 gacaagatag ctgtctagcc gtgtgagtat ctcgattatt ctattgaat
//
LOCUS       DADQRX010000254          751 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.264, whole genome shotgun sequence.
ACCESSION   DADQRX010000254 DADQRX010000000
VERSION     DADQRX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.264"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>751
                     /locus_tag="J8V14_002925"
     CDS             10..>751
                     /locus_tag="J8V14_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922963.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          254 a          131 c          149 g          217 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta g
//
LOCUS       DADQRX010000255          748 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.265, whole genome shotgun sequence.
ACCESSION   DADQRX010000255 DADQRX010000000
VERSION     DADQRX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.265"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>748
                     /locus_tag="J8V14_002926"
     CDS             28..>748
                     /locus_tag="J8V14_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBA0922964.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGGQVGSWFAD"
BASE COUNT          230 a          114 c          188 g          216 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagatg
//
LOCUS       DADQRX010000256          725 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.266, whole genome shotgun sequence.
ACCESSION   DADQRX010000256 DADQRX010000000
VERSION     DADQRX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.266"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..400)
                     /locus_tag="J8V14_002927"
     CDS             complement(14..400)
                     /locus_tag="J8V14_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922965.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          229 a          134 c           86 g          276 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc cttgagatat tcactgatac
      661 ttctactcaa atcatttaaa tttttataat tatttgtacg gaacgtatat atatcaacat
      721 cagtt
//
LOCUS       DADQRX010000257          711 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.267, whole genome shotgun sequence.
ACCESSION   DADQRX010000257 DADQRX010000000
VERSION     DADQRX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.267"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..602
                     /locus_tag="J8V14_002928"
     CDS             <1..602
                     /locus_tag="J8V14_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA0922966.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          181 g          190 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaacgct
      301 tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct
      361 atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg
      421 gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat
      481 tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag
      541 aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat
      601 aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta
      661 gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa g
//
LOCUS       DADQRX010000258          691 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.268, whole genome shotgun sequence.
ACCESSION   DADQRX010000258 DADQRX010000000
VERSION     DADQRX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.268"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..461)
                     /locus_tag="J8V14_002929"
     CDS             complement(<1..461)
                     /locus_tag="J8V14_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA0922967.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          220 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga c
//
LOCUS       DADQRX010000259          614 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.270, whole genome shotgun sequence.
ACCESSION   DADQRX010000259 DADQRX010000000
VERSION     DADQRX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.270"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..234
                     /locus_tag="J8V14_002930"
                     /pseudo
     CDS             79..234
                     /locus_tag="J8V14_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>614
                     /locus_tag="J8V14_002931"
     CDS             253..>614
                     /locus_tag="J8V14_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922968.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEA"
BASE COUNT          251 a           60 c          108 g          195 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagc
//
LOCUS       DADQRX010000260          596 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.271, whole genome shotgun sequence.
ACCESSION   DADQRX010000260 DADQRX010000000
VERSION     DADQRX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.271"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>596
                     /locus_tag="J8V14_002932"
     CDS             <1..>596
                     /locus_tag="J8V14_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA0922969.1"
                     /translation="NIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSH
                     KFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSA
                     ESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVL
                     SLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIF"
BASE COUNT          243 a           50 c          101 g          202 t
ORIGIN      
        1 aaatatagaa gaaaatgata agaattcaat tattgttcat gaagaatttg ctaaacaaaa
       61 caatctaaaa ttaggtgatg aagttgatct tgaattacta gatattgaaa aaagtggaaa
      121 aataaaaagt cataaattta aaattatagg gatcttttct ggtaaaaaac aggaaacata
      181 tacaggatta tcttctgatt ttagtgaaaa tatggtgttt gtagattatt caacaagcca
      241 agagatatta aataattcag aaaataatga aattgcaaat aaaattttaa tgtattctgg
      301 tagtgcagaa tctacagact tagccttaaa caaattgaaa gagcttaaaa ttgatgagtc
      361 aaagtatttt gttgaaaaag attctaatgc gtttgaagag tctttagagt cagtgagtgg
      421 aataaaatat ataataaaag taatgactta ttctattatg ttaggtggga tggttgttct
      481 ttcattaatc ttgattctat ggttaaggga aagaatttat gaaataggta tattcttatc
      541 tattggaaca tctaagatac aaattataat gcaatttata ttcgagttaa tattca
//
LOCUS       DADQRX010000261          596 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.272, whole genome shotgun sequence.
ACCESSION   DADQRX010000261 DADQRX010000000
VERSION     DADQRX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.272"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..106
                     /locus_tag="J8V14_002933"
     CDS             <1..106
                     /locus_tag="J8V14_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922970.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          193 a           93 c           97 g          213 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgtttt
//
LOCUS       DADQRX010000262          593 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.273, whole genome shotgun sequence.
ACCESSION   DADQRX010000262 DADQRX010000000
VERSION     DADQRX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.273"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..580
                     /locus_tag="J8V14_002934"
     CDS             <1..580
                     /locus_tag="J8V14_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA0922971.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DADQRX010000263          537 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.274, whole genome shotgun sequence.
ACCESSION   DADQRX010000263 DADQRX010000000
VERSION     DADQRX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.274"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..365
                     /locus_tag="J8V14_002935"
     CDS             <1..365
                     /locus_tag="J8V14_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBA0922972.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
BASE COUNT          211 a           78 c          117 g          131 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacaga
//
LOCUS       DADQRX010000264          529 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.275, whole genome shotgun sequence.
ACCESSION   DADQRX010000264 DADQRX010000000
VERSION     DADQRX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.275"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(48..350)
                     /locus_tag="J8V14_002936"
     CDS             complement(48..350)
                     /locus_tag="J8V14_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922973.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          181 a           92 c           74 g          182 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      361 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg
      421 gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat
      481 tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaa
//
LOCUS       DADQRX010000265          528 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.276, whole genome shotgun sequence.
ACCESSION   DADQRX010000265 DADQRX010000000
VERSION     DADQRX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.276"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="J8V14_002937"
     tRNA            complement(5..90)
                     /locus_tag="J8V14_002937"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="J8V14_002938"
     tRNA            complement(107..178)
                     /locus_tag="J8V14_002938"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="J8V14_002939"
     tRNA            complement(190..262)
                     /locus_tag="J8V14_002939"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="J8V14_002940"
     tRNA            complement(280..361)
                     /locus_tag="J8V14_002940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="J8V14_002941"
     tRNA            complement(369..441)
                     /locus_tag="J8V14_002941"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="J8V14_002942"
     tRNA            complement(445..517)
                     /locus_tag="J8V14_002942"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DADQRX010000266          514 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.277, whole genome shotgun sequence.
ACCESSION   DADQRX010000266 DADQRX010000000
VERSION     DADQRX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.277"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="J8V14_002943"
     CDS             complement(<1..132)
                     /locus_tag="J8V14_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA0922974.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DADQRX010000267          507 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.278, whole genome shotgun sequence.
ACCESSION   DADQRX010000267 DADQRX010000000
VERSION     DADQRX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.278"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          227 a           70 c           81 g          129 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactc
//
LOCUS       DADQRX010000268          495 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.279, whole genome shotgun sequence.
ACCESSION   DADQRX010000268 DADQRX010000000
VERSION     DADQRX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.279"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..326
                     /locus_tag="J8V14_002944"
     CDS             <1..326
                     /locus_tag="J8V14_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0922975.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          198 a           66 c           90 g          141 t
ORIGIN      
        1 aaaaggcaag tttaaaatca ctaaaagata catggcttga tttatcagaa gataatccat
       61 acagccaatt cttaattact gtaatggctg gtgttaacca attagagcga gatcttattc
      121 ggatgagaca acgtgaaggg attgaattgg ctaagaaaga aggaaagttt aaaggtcgat
      181 taaagaagta tcataaaaat cacgcaggaa tgaattatgc ggtaaagcta tataaagaag
      241 gaaatatgac tgtaaatcaa atttgtgaaa ttactaatgt atctagggct tcattataca
      301 ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc aatattttta
      361 aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa aagccctttg
      421 ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa atgcttataa
      481 attcggccct ataat
//
LOCUS       DADQRX010000269          487 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.280, whole genome shotgun sequence.
ACCESSION   DADQRX010000269 DADQRX010000000
VERSION     DADQRX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.280"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>487
                     /locus_tag="J8V14_002945"
     CDS             <1..>487
                     /locus_tag="J8V14_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922976.1"
                     /translation="KVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDA
                     KWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKE
                     DLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTV
                     EG"
BASE COUNT          197 a           75 c          114 g          101 t
ORIGIN      
        1 aaagtaacct tcaccgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca
       61 gtgaaagacg gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt
      121 tatacagatg cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa
      181 tttgtatcaa gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa
      241 ggtcaaaaag tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac
      301 aaagaagatt taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca
      361 gctggaaata aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt
      421 gaagtagatg ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta
      481 gaaggcg
//
LOCUS       DADQRX010000270          481 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.281, whole genome shotgun sequence.
ACCESSION   DADQRX010000270 DADQRX010000000
VERSION     DADQRX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.281"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            143..400
                     /locus_tag="J8V14_002946"
     CDS             143..400
                     /locus_tag="J8V14_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922977.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          209 a           64 c           68 g          140 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 a
//
LOCUS       DADQRX010000271          476 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.282, whole genome shotgun sequence.
ACCESSION   DADQRX010000271 DADQRX010000000
VERSION     DADQRX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.282"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>476
                     /locus_tag="J8V14_002947"
     CDS             22..>476
                     /locus_tag="J8V14_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922978.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DADQRX010000272          473 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.283, whole genome shotgun sequence.
ACCESSION   DADQRX010000272 DADQRX010000000
VERSION     DADQRX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.283"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..405
                     /locus_tag="J8V14_002948"
     CDS             <1..405
                     /locus_tag="J8V14_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBA0922979.1"
                     /translation="KAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPILID
                     QASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSP
                     YNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          196 a           59 c           63 g          155 t
ORIGIN      
        1 aaagcgttta tcgaatctat tgattctaca atcgatagat taaaaaaaga aattcgattt
       61 tataatagag taattggtat cggaaatatt ataaaaatag tcttatcagc tagtatccca
      121 atattaattg atcaggcttc cgaacacagg tctttactat tgattgtttc aattgcttca
      181 gcaataatta caattataca aagtgggatg tctgcattta attatcaaga taaagtacag
      241 acttcgacag agttattaat gaaaattgaa aaagaaaagt tgttatatat aacgaaaaca
      301 tccccatata ataaaacaga tgaagaaaat ttccatttaa tcgttacaac ccttcaaact
      361 gaattaaatg atattatctc tgattttaat caagttaata actaaaaatt tatttaatta
      421 gaaagtagtg cagataatac taattgtaca attaagaaaa gagataagac ttt
//
LOCUS       DADQRX010000273          460 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.284, whole genome shotgun sequence.
ACCESSION   DADQRX010000273 DADQRX010000000
VERSION     DADQRX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.284"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="J8V14_002949"
     tRNA            complement(20..91)
                     /locus_tag="J8V14_002949"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="J8V14_002950"
     tRNA            complement(113..200)
                     /locus_tag="J8V14_002950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="J8V14_002951"
     tRNA            complement(225..298)
                     /locus_tag="J8V14_002951"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="J8V14_002952"
     tRNA            complement(312..382)
                     /locus_tag="J8V14_002952"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="J8V14_002953"
     tRNA            complement(384..459)
                     /locus_tag="J8V14_002953"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DADQRX010000274          431 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.285, whole genome shotgun sequence.
ACCESSION   DADQRX010000274 DADQRX010000000
VERSION     DADQRX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.285"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>431
                     /locus_tag="J8V14_002954"
     CDS             <1..>431
                     /locus_tag="J8V14_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBA0922980.1"
                     /translation="RSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSI
                     KTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKM
                     YGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          156 a           80 c           72 g          123 t
ORIGIN      
        1 cgttcgcact tagaaaaagc gataaacaca ctcctacgga ctgaattaac ggctttttta
       61 gattacgaaa aatatgatcg cactggtttt aattcaggta attcgagaaa cggttcttac
      121 tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg aaatacctag agatcgtaat
      181 ggtgagttta aacaacaaac tttaccagcc tacaaaagaa caaacgatac attggaaacc
      241 actattatcc atttattcga aaaaggtgtt acgatgtctg aaattgctga tttgatcgaa
      301 aaaatgtacg gtcatcacta tactccacaa accatgtcca acatgactaa agttctgact
      361 gaagaagtaa atgcctttaa atccagagcc ttaaatgata agtatgtcgc tatttttatg
      421 gacgctactt a
//
LOCUS       DADQRX010000275          430 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.286, whole genome shotgun sequence.
ACCESSION   DADQRX010000275 DADQRX010000000
VERSION     DADQRX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.286"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>430
                     /locus_tag="J8V14_002955"
     CDS             <1..>430
                     /locus_tag="J8V14_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBA0922981.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DADQRX010000276          426 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.287, whole genome shotgun sequence.
ACCESSION   DADQRX010000276 DADQRX010000000
VERSION     DADQRX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.287"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>426
                     /locus_tag="J8V14_002956"
     rRNA            <1..>426
                     /locus_tag="J8V14_002956"
                     /product="16S ribosomal RNA"
BASE COUNT          111 a           96 c          133 g           86 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 cccctt
//
LOCUS       DADQRX010000277          421 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.288, whole genome shotgun sequence.
ACCESSION   DADQRX010000277 DADQRX010000000
VERSION     DADQRX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.288"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            188..385
                     /locus_tag="J8V14_002957"
     CDS             188..385
                     /locus_tag="J8V14_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922982.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          164 a           57 c           95 g          105 t
ORIGIN      
        1 tatgaattcg ttgggatgag tgtgcgcaaa aatggacggt tagttattaa atatgcgaaa
       61 gataaccatg caaagcggtt tatgcgagag gagaatcgaa aaattgacca aaaagaattc
      121 aggcaacagt atgaagaaga aaaagaaaaa acttttaaag caattcgctg ggattgggaa
      181 cgccaacgtg aaatttatat gtctaagttg tggtgaaaaa gaatcaatcc caatggatgt
      241 agtaaaattt ttagatgatg cagatattgc aaatgatccg aacaatcctc cccaatttac
      301 ctgtgaaaaa tgtgggaagg agatgtatcc agaatattat agaaacgccc ttggaataga
      361 gtttaagatc agtgatgttc aataagaagg ttaatcatac aaaaagtcca ccggagaatt
      421 c
//
LOCUS       DADQRX010000278          421 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.289, whole genome shotgun sequence.
ACCESSION   DADQRX010000278 DADQRX010000000
VERSION     DADQRX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.289"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..355)
                     /locus_tag="J8V14_002958"
     CDS             complement(170..355)
                     /locus_tag="J8V14_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HBA0922983.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DADQRX010000279          418 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.290, whole genome shotgun sequence.
ACCESSION   DADQRX010000279 DADQRX010000000
VERSION     DADQRX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.290"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>418
                     /locus_tag="J8V14_002959"
     CDS             <1..>418
                     /locus_tag="J8V14_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBA0922984.1"
                     /translation="YRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTK
                     GELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPI
                     TYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          140 a           77 c           71 g          130 t
ORIGIN      
        1 tatagagcgg tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc
       61 actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag
      121 aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa
      181 ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca
      241 acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt
      301 ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta
      361 tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DADQRX010000280          412 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.291, whole genome shotgun sequence.
ACCESSION   DADQRX010000280 DADQRX010000000
VERSION     DADQRX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.291"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..340
                     /locus_tag="J8V14_002960"
                     /pseudo
     CDS             69..340
                     /locus_tag="J8V14_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          144 a           58 c           75 g          135 t
ORIGIN      
        1 gtttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
       61 accacgggtt aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata
      121 aaccttttcc ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac
      181 gatttatatt ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt
      241 tggtttagct aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat
      301 tgattctcct caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg
      361 atagggtctc acgctctttg tttttcgcca tttaaagatt gaaatttttg cc
//
LOCUS       DADQRX010000281          386 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.292, whole genome shotgun sequence.
ACCESSION   DADQRX010000281 DADQRX010000000
VERSION     DADQRX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.292"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>386
                     /locus_tag="J8V14_002961"
     CDS             <1..>386
                     /locus_tag="J8V14_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922985.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DADQRX010000282          386 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.293, whole genome shotgun sequence.
ACCESSION   DADQRX010000282 DADQRX010000000
VERSION     DADQRX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.293"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>386
                     /locus_tag="J8V14_002962"
     CDS             <1..>386
                     /locus_tag="J8V14_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922986.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DADQRX010000283          381 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.294, whole genome shotgun sequence.
ACCESSION   DADQRX010000283 DADQRX010000000
VERSION     DADQRX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.294"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="J8V14_002963"
     tRNA            complement(21..94)
                     /locus_tag="J8V14_002963"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="J8V14_002964"
     tRNA            complement(108..181)
                     /locus_tag="J8V14_002964"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="J8V14_002965"
     tRNA            complement(209..281)
                     /locus_tag="J8V14_002965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="J8V14_002966"
     tRNA            complement(301..374)
                     /locus_tag="J8V14_002966"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DADQRX010000284          369 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.295, whole genome shotgun sequence.
ACCESSION   DADQRX010000284 DADQRX010000000
VERSION     DADQRX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.295"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..>369
                     /locus_tag="J8V14_002967"
     CDS             98..>369
                     /locus_tag="J8V14_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922987.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DADQRX010000285          368 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.296, whole genome shotgun sequence.
ACCESSION   DADQRX010000285 DADQRX010000000
VERSION     DADQRX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.296"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..272)
                     /locus_tag="J8V14_002968"
     CDS             complement(<1..272)
                     /locus_tag="J8V14_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922988.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DADQRX010000286          368 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.297, whole genome shotgun sequence.
ACCESSION   DADQRX010000286 DADQRX010000000
VERSION     DADQRX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.297"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>368
                     /locus_tag="J8V14_002969"
     CDS             82..>368
                     /locus_tag="J8V14_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBA0922989.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DADQRX010000287          362 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.298, whole genome shotgun sequence.
ACCESSION   DADQRX010000287 DADQRX010000000
VERSION     DADQRX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.298"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>362)
                     /locus_tag="J8V14_002970"
     rRNA            complement(<1..>362)
                     /locus_tag="J8V14_002970"
                     /product="16S ribosomal RNA"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DADQRX010000288          344 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.299, whole genome shotgun sequence.
ACCESSION   DADQRX010000288 DADQRX010000000
VERSION     DADQRX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.299"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>344
                     /locus_tag="J8V14_002971"
     CDS             <1..>344
                     /locus_tag="J8V14_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBA0922990.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DADQRX010000289          339 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.300, whole genome shotgun sequence.
ACCESSION   DADQRX010000289 DADQRX010000000
VERSION     DADQRX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.300"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>339
                     /locus_tag="J8V14_002972"
     CDS             <1..>339
                     /locus_tag="J8V14_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBA0922991.1"
                     /translation="ETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRL
                     VKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNK
                     FYRSLRTASTT"
BASE COUNT          119 a           66 c           65 g           89 t
ORIGIN      
        1 tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca tcgatgcaga
       61 tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag cctatgcttt
      121 tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtcacag ataaagcccc
      181 ctctattaca agtgccttta agaaactaaa agaatacggc ttttatcaag ggacagaaca
      241 tcgtaccatt aaatacctga ataatttgat tgaacaagac catcgtccag taaagagacg
      301 caataaattc tatcgaagtt tacgcactgc ctctaccac
//
LOCUS       DADQRX010000290          321 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.301, whole genome shotgun sequence.
ACCESSION   DADQRX010000290 DADQRX010000000
VERSION     DADQRX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.301"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>321
                     /locus_tag="J8V14_002973"
     CDS             <1..>321
                     /locus_tag="J8V14_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBA0922992.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DADQRX010000291          317 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.302, whole genome shotgun sequence.
ACCESSION   DADQRX010000291 DADQRX010000000
VERSION     DADQRX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.302"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          111 a           39 c           51 g          116 t
ORIGIN      
        1 ggcagtggta tttcttcagt aagattaaaa gagaaagaat atactcaaaa gattattcat
       61 attcctaagc ctgaaattaa gcgttttgag ataaaagact attctgcttt agggctgttc
      121 tcaattggtt actggtgata aaaaataaga atactaaaac tatacgtaga atataagtaa
      181 tcggcatcaa tcctaattat gtgattgaat gccgattttt gttacgcgct atttttaatt
      241 atctgtaaga agaataattt tattttactc atattcgttt tcactttgaa gaagtaaatt
      301 gcaatttctt ataagtt
//
LOCUS       DADQRX010000292          315 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.303, whole genome shotgun sequence.
ACCESSION   DADQRX010000292 DADQRX010000000
VERSION     DADQRX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.303"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>315
                     /locus_tag="J8V14_002974"
     CDS             <1..>315
                     /locus_tag="J8V14_002974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922993.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DADQRX010000293          313 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.304, whole genome shotgun sequence.
ACCESSION   DADQRX010000293 DADQRX010000000
VERSION     DADQRX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.304"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>313
                     /locus_tag="J8V14_002975"
     CDS             10..>313
                     /locus_tag="J8V14_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA0922994.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT           89 a           61 c           63 g          100 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata ccaatgtcgc ttaccgctgg
      301 tttttaggct act
//
LOCUS       DADQRX010000294          299 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.305, whole genome shotgun sequence.
ACCESSION   DADQRX010000294 DADQRX010000000
VERSION     DADQRX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.305"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>299
                     /locus_tag="J8V14_002976"
     CDS             <1..>299
                     /locus_tag="J8V14_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBA0922995.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DADQRX010000295          285 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.306, whole genome shotgun sequence.
ACCESSION   DADQRX010000295 DADQRX010000000
VERSION     DADQRX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.306"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..285
                     /locus_tag="J8V14_002977"
                     /pseudo
     CDS             1..285
                     /locus_tag="J8V14_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          111 a           58 c           54 g           62 t
ORIGIN      
        1 tcaaacaaca aatcgtcgat ctctatctcg ctggtaagcc tcgcgcagaa attattcgag
       61 agtatgagct tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct
      121 cattcaaaga aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga
      181 aaaataagca actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac
      241 gaaaagacaa gtaattgatg ccaacaagca taaatattcc atatc
//
LOCUS       DADQRX010000296          269 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.307, whole genome shotgun sequence.
ACCESSION   DADQRX010000296 DADQRX010000000
VERSION     DADQRX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.307"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="J8V14_002978"
     CDS             51..260
                     /locus_tag="J8V14_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0922996.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
BASE COUNT           97 a           27 c           48 g           97 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaac
//
LOCUS       DADQRX010000297          255 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.309, whole genome shotgun sequence.
ACCESSION   DADQRX010000297 DADQRX010000000
VERSION     DADQRX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.309"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           96 a           30 c           55 g           74 t
ORIGIN      
        1 actttaagga gcatatttta gttgcgccat gatttcttgc tgtaaagaag gagcgataaa
       61 tattctaaaa taatttttat agtcaataaa aagcgatgat ttaaagttgg gataatgata
      121 cttccgtaag gctcgccgta aacttgggga gaggtgaaat tcctatgtgc ttttgacaaa
      181 agcagctgat agaaataaat gaaaaaaagt aaggaaggtg ctataatgaa aacagctact
      241 cgaaaagatt tgatt
//
LOCUS       DADQRX010000298          252 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.denovo.310, whole genome shotgun sequence.
ACCESSION   DADQRX010000298 DADQRX010000000
VERSION     DADQRX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.denovo.310"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..>252)
                     /locus_tag="J8V14_002979"
     CDS             complement(32..>252)
                     /locus_tag="J8V14_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA0922997.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DADQRX010000299        21585 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.045, whole genome shotgun sequence.
ACCESSION   DADQRX010000299 DADQRX010000000
VERSION     DADQRX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.045"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1056)
                     /locus_tag="J8V14_002980"
     CDS             complement(<1..1056)
                     /locus_tag="J8V14_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBA0922998.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQI"
     gene            1315..2817
                     /locus_tag="J8V14_002981"
     CDS             1315..2817
                     /locus_tag="J8V14_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA0922999.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2819..3556
                     /locus_tag="J8V14_002982"
     CDS             2819..3556
                     /locus_tag="J8V14_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA0923000.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            3562..3720
                     /locus_tag="J8V14_002983"
                     /pseudo
     CDS             3562..3720
                     /locus_tag="J8V14_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(3727..4920)
                     /locus_tag="J8V14_002984"
     CDS             complement(3727..4920)
                     /locus_tag="J8V14_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA0923001.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(4999..5205)
                     /locus_tag="J8V14_002985"
     CDS             complement(4999..5205)
                     /locus_tag="J8V14_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA0923002.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(5198..6340)
                     /locus_tag="J8V14_002986"
     CDS             complement(5198..6340)
                     /locus_tag="J8V14_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBA0923003.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            6852..7487
                     /locus_tag="J8V14_002987"
     CDS             6852..7487
                     /locus_tag="J8V14_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0923004.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            7641..7718
                     /locus_tag="J8V14_002988"
                     /pseudo
     CDS             7641..7718
                     /locus_tag="J8V14_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7963..8079
                     /locus_tag="J8V14_002989"
     CDS             7963..8079
                     /locus_tag="J8V14_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBA0923005.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8095..10014
                     /gene="tet(M)"
                     /locus_tag="J8V14_002990"
     CDS             8095..10014
                     /gene="tet(M)"
                     /locus_tag="J8V14_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBA0923006.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10133..10300
                     /locus_tag="J8V14_002991"
     CDS             10133..10300
                     /locus_tag="J8V14_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBA0923007.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(10360..10713)
                     /locus_tag="J8V14_002992"
     CDS             complement(10360..10713)
                     /locus_tag="J8V14_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0923008.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            11217..11636
                     /locus_tag="J8V14_002993"
     CDS             11217..11636
                     /locus_tag="J8V14_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBA0923009.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            11662..11868
                     /locus_tag="J8V14_002994"
     CDS             11662..11868
                     /locus_tag="J8V14_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA0923010.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(12096..12269)
                     /locus_tag="J8V14_002995"
     CDS             complement(12096..12269)
                     /locus_tag="J8V14_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBA0923011.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            12245..12910
                     /locus_tag="J8V14_002996"
     CDS             12245..12910
                     /locus_tag="J8V14_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HBA0923012.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(12992..14158)
                     /gene="dnaJ"
                     /locus_tag="J8V14_002997"
     CDS             complement(12992..14158)
                     /gene="dnaJ"
                     /locus_tag="J8V14_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBA0923013.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(14309..16138)
                     /gene="dnaK"
                     /locus_tag="J8V14_002998"
     CDS             complement(14309..16138)
                     /gene="dnaK"
                     /locus_tag="J8V14_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBA0923014.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(16189..16752)
                     /gene="grpE"
                     /locus_tag="J8V14_002999"
     CDS             complement(16189..16752)
                     /gene="grpE"
                     /locus_tag="J8V14_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBA0923015.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(16846..17889)
                     /gene="hrcA"
                     /locus_tag="J8V14_003000"
     CDS             complement(16846..17889)
                     /gene="hrcA"
                     /locus_tag="J8V14_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBA0923016.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(18123..19288)
                     /locus_tag="J8V14_003001"
                     /pseudo
     CDS             complement(18123..19288)
                     /locus_tag="J8V14_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(19304..19531)
                     /locus_tag="J8V14_003002"
     CDS             complement(19304..19531)
                     /locus_tag="J8V14_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBA0923017.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            20015..20605
                     /locus_tag="J8V14_003003"
     CDS             20015..20605
                     /locus_tag="J8V14_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBA0923018.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            20795..21508
                     /locus_tag="J8V14_003004"
     CDS             20795..21508
                     /locus_tag="J8V14_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0923019.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         6381 a         4213 c         3988 g         7003 t
ORIGIN      
        1 gatttgaggc atggttacgt gcgattcgtc cgccacgatc aaaaaatctt caggaaagaa
       61 atccaatagc gtataaggcg gttcaccttc tttacgtccg tccatatgcc gagaataatt
      121 ttcaattccc gacgtgtagc ccatttctaa catcatttca atatcgtaat tggttcgctg
      181 ttctaaacgt tgagcttcca gaagtttgtt ttcatttcgc aagacagtta aacgctgttc
      241 taattcctct ttgatattcg ctacggcatg ctccatatgt tcatcattcg tcacaaagtg
      301 agtcgccggg aagattgcta cgtgttccgt ttctcctaaa acttcacctg tcagtgcatt
      361 gacttctcgg atacgttcga cctcatctcc aaaaaactcc acacgtaagg cacgttcatc
      421 tcttgaagcc ggaaaaatct ccacgacatc accgcgcact cgaaaacgac cgcgctgaaa
      481 atctatatca tttctttcga attggatact gactaagtca cgaatcaact gattgcgatc
      541 tagttctgct ccctgtcgaa tcgaaactac ttgtttttgg tactcaaaag gcgagcctaa
      601 tccaaaaata catgaaacgg atgcaatgac gatcacatca tttctttcta ataatgaact
      661 agtagccgaa tgtcgtaatt tgtcgatctc atcatttaca cttgaatctt tttcgatata
      721 cgtatcgctt gaaggcacat aagcttcagg ctgataataa tcataataag aaacaaaata
      781 ttcaacagca ttgttaggga aaaattcctt gaattccccg tacagctgac cagccaatgt
      841 tttgttatgg gcaataatca gtgttggttt attcactttc tcaataagat tggaaatcgt
      901 atatgttttc cctgttccag tcgcacctaa taagatttgt gcttttttcc cgccgactac
      961 gccatccact aattggttaa tcgcttcagg ctgatcacca gccggttggt atttcgagac
     1021 aagctcaaag tgtcgggagg tatctctctc aatcattctt cgtcctcctt ttcattcatt
     1081 ccttttcatt ttacgaccaa taaaaagaaa aggaaagcaa aggcattcct tactaaaaaa
     1141 ttataccaca ccgaacatac tttcgccact tttttatctt attatacatg ttatgtttta
     1201 taacataaaa tgttagaaac tcgcattttt ttcatttttt cgagcttaca gtcttgtctg
     1261 attcagcact tttcgcctat aataataaaa tattggaaat ttaggaggtt gattatgaaa
     1321 agaaagcatt tatttcactc atttgttttc ttaatgacag gacttgtgac acttgcgttt
     1381 ggtcatgttg cacaagctga caacactaca acggcagagt caagcacaag cgagacaatg
     1441 caaaaaatag agcccaaaaa agacgtatac gtaattgcca gtgactctgc ttttgctcca
     1501 tttgaatttc aaaacgcaaa gggcgagtac gaaggcatcg atgtaaaatt gatggaaaac
     1561 atcgccaaac tgcaaggatt taacatccga atggactacc gtggtttctc tgctgctttg
     1621 caagctttgg aatcaggaca agcagacgga atgattgccg gaatgactgt aacagatgaa
     1681 cgaaagaaaa gctatgattt ttctgattct tactttgata gcgggatcca attagctgtc
     1741 aaaaaaggag atacttcgat caagtcttat gaagatttga aaggaaaaac agtcggagca
     1801 aaagttggta ccgaaagtgc tgaattttta agcgaccatg aatctgaata tggctattct
     1861 atcaaatatt ttgatgctgc tgatcaattg tacgacgggt taaaagttgg cgcaatcgat
     1921 gcaatgatgg atgattatcc ggtaatcggt tatgcgatca aacaaggaca agaattggaa
     1981 acaccgatca aaaaagaatc tggcggaaaa tatgcatttg ccgttaaaaa aggacaaaat
     2041 cctgagcttc ttaatatgtt caatgaagga ttgaaagaat taaaaagaac aggcgaatac
     2101 gataagatta ttaaagaata tgtagcggat ggttcagaaa cttccagtga gtcttccact
     2161 gctgatgaat caacatttct tggacttatc caaaacaact atcaagcatt gttgaaagga
     2221 ctttggagaa caattctcct aactttgatc tcctttgctt tggctatagt aatcggtgtg
     2281 atttttggat tattcagtgt agcaccaata cgcgctcttc gcacaattgc tagtatttat
     2341 gtcgatatca tccgaggaat tccaatgatg gttcttgcgt tctttatttt ctttgggttg
     2401 cctggtatcg ttggatttac gattcctgac ttccttgctg gtgttatcac cttgactctt
     2461 aacgcaagtg cttatatcgc agagattgtt cgaggcggga tcaacgccgt tcctgtcgga
     2521 caaatggaag cttccagaag tttaggtctt tcttataatc ggacaatgca aaaaatcatt
     2581 ttgccgcaag cgatacgcat catgatccca tcattcgtca atcagttcgt tatctcatta
     2641 aaagacacaa cgatcatttc cgcaatcggt gtagtcgaat tgctgcaaac tggtaagatc
     2701 attgttgcac gtacaacaca aagtacgtat gtttacttga tcattgcgat catgtacttg
     2761 attttgatca cagcattgac aaaattagca aaagttttag aaaagaaggt gaagtaagat
     2821 ggcagaaaaa atcttagttg agcatttagt caaaaaatac ggcgataaca cagtattaaa
     2881 cgacatcaat gtatccatca acgaaggaga tgtcgtctgt atcattggcc cttcaggttc
     2941 aggaaaaagt acattcttgc gttgcttgaa tcgtttagaa gaaccttctg acggtgaaat
     3001 tattattgac ggcgcccact tgatggataa aagcacagat atcaacttgg tgagacaaca
     3061 tatcggaatg gtattccaac actttaacct attccctcat ctttctattt tggaaaacat
     3121 cattttagca ccgatggacc tgaagaaaga atctagagaa gaagcagaga aaaaagcaat
     3181 caaattactg cagacagttg gtctagaaga taaaaaagat atgtatcctg aaatgctttc
     3241 tggtggacaa aaacagcgtg tggctattgc tcgtgctcta gcgatgaacc ctgacatcat
     3301 gctttttgat gagcctactt ctgcactcga tcctgaaatg gtcggtgatg tcttgaacgt
     3361 catgaaaaag ttagcagaac aaggtatgac gatggttatc gttacgcacg aaatggggtt
     3421 cgctaaagaa gtggccaatc gcgtcatgtt cattgacgac ggaaactttt tagaagatgg
     3481 aacgcctcaa caaatctttg aaaatccgca aaatgaacgg acaaaagatt tcttgaataa
     3541 agtattaaat atttaaaata aattatatat gtctttttga ctatcttttt atatcttctt
     3601 aaatgtccgc aaagccttat tttataggct ctacgggcat tttgcttttg tggtaaacct
     3661 cacatatcta ggtctatctt cttatatttt cactatcaag cgtggttaaa atcgtggtaa
     3721 atacttctat gcaattagac gctgaacctc tgattttgcg gtatctatgg acgcatgagc
     3781 ataccagttc attgtgatac tgatatttga atgccccatg atatactgta aatcttttgg
     3841 gttcatgttc ttgcttgcca gtcttgtgca gaatgtatgg cgtagcgtat gcggtgtgat
     3901 atgtggcaat ggattgtcct tgtggtgctt gttgtatttc tttaccatac ggataaataa
     3961 ggcgttataa tcaatcgcaa ctttgggctt gcccttatga ttgacaaata ggaagttgcc
     4021 ccgtccgtct atcacaaatg gttctgcctt tggacgtttt ttcataacct gttgaaatgc
     4081 ctgtatcgtt tctttgctta atggtacttg ccttattccg ctttttgttt taggcgtttc
     4141 aatataatag ccctgttcct tgctctttag taactggtgg tcgataatca caacttcatt
     4201 cttgaaatca atgtcggcta ctgtcagtcc gcacagttcc gaaatacgaa gtcctgtctt
     4261 taacagtatc agcacatcat cataatgctt gtgatacaca ttgtccgtct tgatgaatga
     4321 gagtaaggct tgttcctgtt cctctgtcaa tgcgactttc tttttggtat cattttctag
     4381 gacttcactc aacttgaaat caaagggatt tttcctcaca taatcatctt gaatggctat
     4441 gtagaatgac gcttttaacg agcgtttatg gttgttaatg gtattgtagg aaaagccttt
     4501 gtctttcatg cgtaacgccc attccttagc gtcagagggt ttgattgtat caatgctcct
     4561 agcacctaac ttgtcctctt tcaataaccg catgagttgt tcccgttgtt tctgtgtgct
     4621 tttcttcaca tttgccctct gtgcgttctg tttggcataa agttggcaaa gcgtcatttt
     4681 cttgccagtg ctgtcgatac cgtcctcaat atcccgtctt atctgctgtt ccagttctcg
     4741 aagtgagggt ttttcccgtt ttccctttgg tgtcgggtct gtgggtgtca atctccaagc
     4801 atacacatat tttgtgtttc caaatgcgtc tacatattta tataagtatt ttctgtctgt
     4861 tcgttggctc tctccagtat gcaggatacg acctttgtta tcccgtcttt tttctttcat
     4921 ggtgtctgct cctttccttg ttggaaagag ccttgatatg acttgtgttc atcataacac
     4981 atacaaggct catttgcatt agatagcgtc caatttatca atgacctgtt caaactgtcg
     5041 gcgtttaatc tgtatgcggt tgccattcat aatgagccag ccagcgtcct tgttttcctc
     5101 tgccaatcgt cttaacttgt tttctccgat acggaaatac tttgacgctt cttcaatggt
     5161 aagggtgtat ttttcccata cgggaatatc ctgtgcatta ctcataaaac accccctcac
     5221 tttcgattac gtctgctatg tccgtacaca tctgttccac aagcatttcc tgcttttcag
     5281 agaggtgggt acattcatca atcattttca tgtagtttga gcctgtcagc ctgtcgattt
     5341 cctctatcat cacgattgac ggcatgacct gtctttccag ccattgcaga gtgcgttcca
     5401 tattgacagg tacggggttc atttcaaaag gtaaactgtc atgggaaatg aatatctccc
     5461 acagcggata gtcgggaaat tgcacaaaat cagtaataag gtaaaacacc agcccatgag
     5521 ggttatatgt caataacaat tcctcaactg cctgtactgc ttttgtacca cgcagacgaa
     5581 tttcaaaacg attgataata tccgtatgtt tctttttggt tgcctgttcc ttttgttttt
     5641 catacaggca gaagtatttt gtgcttgcct ttgaaccgat ataaagggtg cttgctaaat
     5701 tacggttttt tccgcttagt tttccaccct gcacattttc ataattttta cagcgacaat
     5761 ccgcaccgcc atttctgtat ttttcagaca aaacgggaat atccaacaga ccacacatat
     5821 cattgattgc caagtcaaaa cgtcggataa caccgtcaca gtcaagacag cggtttaaaa
     5881 atgaatacca atctctgttc tgtgcctgca agacatattc catgttgcgt gagcctgccc
     5941 cttttaattc caagaatacg cccatgtgtt cgtcatcaga agccatgacc ttgatttctc
     6001 cgtaggcgta atgctcttta tagccataat acccatagtc ataatggata aaataatctg
     6061 ctttcatgcc aagcaccttt cggataattt ccaatgcgtc agtcgttgga taacggacag
     6121 acagataatc aacgagcaga taaaaaggct tatcgcagat ataataattc agacaacgca
     6181 ggataatatt ttgtgtttcc ttgctggcgt tggcttttcc gttctcaaag cggttgatac
     6241 tatatcttga tacatgggta agacttgcaa gttctccttg tgaaagaccg cttgcaagac
     6301 gtttgttctt catttcaagt gcaaattcta aattgttcat tgtattcctc caatcccaca
     6361 atagccttga aatacctgta aacctctatt caaaaagtgt gatatgcact atcttcttaa
     6421 aacttctcat tactactcac aactttggga ttgtatggtt tttgacttgt cttttgtgaa
     6481 atagtgcccc cctgttagat aacgggggca cagacaaggc tcgcaggctc gccagccgac
     6541 aaagcatttc ttccccttga cacaaggtgt catggcggta agcctgagag gcttccgcca
     6601 ccgtgtcaga aatactttgt cagcaagttc tgttttgcct tttctaacaa gttccatttc
     6661 aaccgatatt catatctacg gatttaatca gataggtatt tctttttgaa tgtttttata
     6721 tttcattcta ttgtgcttat tcgattacaa gaacatagta attcttatat gcttatttgt
     6781 caatatcttt ttttgccttt acaaatcata ttaagcatga tagacttata atagcgaaga
     6841 tataacgaga tatggagggt tatgcaatga aaataagcca tttaggaaat aatatacaga
     6901 ccataagaaa atttagggga atgaaacaac aggaacttgc ggacaaaatc ggtatcaata
     6961 tgcagagcct ttccaagatt gaaagagggt tgaactatcc tgcctatgaa acgctggaaa
     7021 agataatgga agtgctggac gttacgccca atgaattgtt gtcgggagaa tggaagtatg
     7081 tcaatcaatc tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt ctaaatgcag
     7141 aactgaaaca tggacactat gataacttct ttgacagcga ggaagaatgg ctggaatatg
     7201 agttagaaaa gttacgggaa tatattacag actatatcaa cgggaaaagc attgaagcgt
     7261 ccgaccttta cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa ttattagacc
     7321 gctatgatga tttatacagt atggatatgt ttggggaaag catagaggga cataagtata
     7381 agacacctta tcaagtcgtg aaaatgataa acccgaactc aaaagaagat atggaactgt
     7441 tacgagaagt attaaggaat aacaattttg atgatgagga cgagtaactt ttttcctctt
     7501 gcgagaagat acagcaaaca aaaagcccga tcaagagtgc aaagcaccac caatggtggc
     7561 aaagctcttg acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa gaaagggata
     7621 aaatctgatt tgtggtaaat caggcaaagg caacgcaggt atctcaattt gatttggtac
     7681 ttgaaaagaa cgggagtaat tggaagatta tagaataaca aatattggta cattattaca
     7741 gctattttgt aatcacgtac tctctttgat aaaaaattgg agattccttt acaaatatgc
     7801 tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg cggcaaggta
     7861 ttcttaaata aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc ttttttagga
     7921 gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatccg
     7981 gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag tgataaaagt
     8041 atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa
     8101 attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc
     8161 ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg
     8221 acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct
     8281 tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta
     8341 gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa
     8401 gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc
     8461 acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag
     8521 gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct
     8581 aatatgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac
     8641 gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa
     8701 gaggaaagca taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa
     8761 agtaatatag ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca
     8821 catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga
     8881 caacgtcttg catatatacg cctttatagt ggagtactac atttacgaga ttcggttaga
     8941 gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata cttcaataaa tggtgaatta
     9001 tgtaagattg atagagctta ttctggagaa attgttattt tgcaaaatga gtttttgaag
     9061 ttaaatagtg ttcttggaga tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg
     9121 caccctctac tacaaacaac tgttgaaccg agtaaacctg aacagagaga aatgttgctt
     9181 gatgcccttt tggaaatctc agatagtgat ccgcttctac gatattacgt ggattctacg
     9241 acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat tagtgcactg
     9301 ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc ctacagtcat ttatatggag
     9361 agaccgttaa aaaatgcaga atataccatt cacatcgaag tgccgccaaa tcctttctgg
     9421 gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca gtatgagagc
     9481 tcggtttctc ttggatactt aaatcaatcg tttcaaaatg cagttatgga ggggatacgc
     9541 tatggctgtg aacaaggatt gtatggttgg aatgtgacgg actgtaaaat ctgttttaag
     9601 tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt
     9661 gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt
     9721 aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt
     9781 gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct
     9841 gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt
     9901 tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt
     9961 cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt
    10021 ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaga
    10081 tttgaattaa agtgtaaagg aggagatagt tattataaac tacaagtgga tattgtgtcc
    10141 tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa aaaaattccc
    10201 cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac
    10261 tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta atacaatctc
    10321 attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga tgtttcttgc
    10381 ttcgttgata ccgctggcta aagattccat taaggatagt tctttgtctg taaagctatc
    10441 catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag caggtaagaa
    10501 aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt gtatgctggg
    10561 gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc
    10621 cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc
    10681 tctaaaatca tatttatctt cttttttacg catagtagac cacctctata cattttattg
    10741 ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata ggtttatatt
    10801 taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt gctatttacg
    10861 ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg
    10921 aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa agtcgtctat
    10981 ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat agaataacag
    11041 aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc agaacttttt
    11101 tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga ctaagagtga
    11161 aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt gagattatga
    11221 ggacatcttc tttcgagcat atcgtcagat tacagttcaa ttctttaatg ctgattgtca
    11281 ttaagaacaa agtgaaaagt cgaaacagac agcttgcaag acattctaaa cgtgaagtat
    11341 tgttttgtga aatagccgaa acgaaacaag ttgaacgtgg aacgattgat acctgttttt
    11401 atgattgtgt atcatttaaa gtcttgcact ttacaattta tgtatctgat gaaacacttg
    11461 gtacagtttt acatagacta tcagaaaaac agcgtagtgc gattttatta cgttattttc
    11521 aaggaatgaa cgacagagaa atttcagaac tgtaccatgt atcacgttct gctatttgca
    11581 gtagaagaac tagaggacta aggaagttaa aaatgctttt gagcgaaagg aagtaaagat
    11641 tatggctatc cctcttttga attgatttgc aaagcgtcaa gcggagatga aatggcagtt
    11701 aaagagattt tgaagtttta tgacggttat atttctaaat tatgcttgcg tcctttttac
    11761 cactctgaaa gtggtaaaat cattatgcag gttgatgaag aactaaaagg agaaattcat
    11821 acagacatga tgaaagcaat attgaaattt gaaatcggag tgaagtaatc aactgtataa
    11881 aaaaatcggg agatacacag tttcgtgtta tctcccttag tagttttacg gtcatatcaa
    11941 ggctcattca atcgtggtaa attcgtggta agatgagtgt ttttctatac ttcaaaatgc
    12001 ttgaatgtat agtgtttatg cggataattg aaaattagat ataaaattta ataaaaacca
    12061 aaaatgcacg gtcgccatga agggaccgtg tatttttatc ttcttatttt ctcacctaat
    12121 cgtatcagcc gcatcgcttg ttttcctgca caaacaagca atcgctctac ctgttccatc
    12181 gcataagaaa gtaccatcgg ttcattgatg atatcaagta catagtcgat accaccctca
    12241 cttaattgag cttgattttt cttctccata ttatgtagtt gtgggacaac tttctgacaa
    12301 tcagcaattg tgtcaagaac ggatcaatcc tttattggag ttgctcgaaa gggtcttctc
    12361 ttattacgga caggctttgc tcactgtaca tagacaacaa atcattatcc tagtcaatcg
    12421 gatttccaga gcctcattgc tatcattgct tgataaaatc cagacaaagt ttaataaaat
    12481 gtatcagtta cagctaaact ttggaattgg gtcgctttgt tatacagagc aagagacacc
    12541 gcaatctttc cttcatgcca aacaagtttg cgaatggata gctttccatc aatctgtgaa
    12601 cgagatccgc ttttttgaag atttagacct cgggatcgtt ttaccagcaa tcccgtcaga
    12661 tcaacgaaca ttatatgtaa aacgtatttt gaaaagcctg acagaagaag aagtacattt
    12721 gttcaagaag actttggcat gcttcagtaa aaataacgga agcatcaaaa attgctcaga
    12781 agaattattt attcataaaa acaccttgca atatcgattg aacaagttcc attctctcac
    12841 tggttatacc cctagaaact atgacgatta tcatattcta aagctggctt ttttgttggt
    12901 acaaacatga caactacaaa aaaaccaggc gaacatatga ttgttcgtct ggtttttaat
    12961 tattaatgac ttagcgtttt tactagaaaa actacttccg ctttttcccg ccaaaagcat
    13021 ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg ccgccagctt
    13081 cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt gtgatcagtt
    13141 ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg ccttttccac
    13201 gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc acatctccat
    13261 ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt aaaggcaatt
    13321 catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca cggaagacca
    13381 catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca gctaaacgca
    13441 tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt gctttcttct
    13501 catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct gtcccatgac
    13561 aaacatcaca agtttgacgg ctcatcactc gtccaagtgg tgtttggcgt tctacattaa
    13621 tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt tttgcaccgt
    13681 tcccaccaca agtatgacag atttcttccc gattatactt gatttctttt tctacaccaa
    13741 aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct tgacgaggcg
    13801 catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata tcttcaaaac
    13861 caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca gctccaccag
    13921 caccgtaatt tggatctgtg cctgcatgcc cgtattggtc atatgcagcg cgtttttgcg
    13981 ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc gcatcaggtt
    14041 ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc tttatttcat
    14101 cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt gttgccatag
    14161 gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc atattttgtt
    14221 tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag tagcaacttt
    14281 ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact tcttcaaaat
    14341 cagcatcaac gacatcatct gctccgcctt gagctgcttc tgggttttct tgagcttgtt
    14401 gttgtgccgc ttgttcgtat agtttaacag tcaagttttg aacgatttcg ttcaatgcat
    14461 cgcgtttagt tttcatttct tcgatgttgt ttgcttcaac agcggctttc aattcatcgc
    14521 gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat tctttcaatg
    14581 ttttgtccac agagaatagc aatgcatcta catcattacg taggtcaact tcctctttac
    14641 gagctttatc tgcttctgca tttgcttctg catcttttac catacgttcg atttcttcgt
    14701 ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta cctagatctt
    14761 tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg atttgtggaa
    14821 taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt ttgttgtcag
    14881 cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga ttatctgccg
    14941 cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca atcaatttag
    15001 tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca agtaaaacaa
    15061 catctttcac atcaccagtg atcacgccac cttggatagc agcacccata gcaactactt
    15121 catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact gcttctacaa
    15181 ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct gattgtgata
    15241 aacctgcatc ttttagcgct tgacgtactg gtacttttgt acgttctact aagtcagctg
    15301 ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt ccagattctc
    15361 ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa tcttttttcg
    15421 ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac aagtcaatgc
    15481 tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca aagtcgtccc
    15541 cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct cctagttcaa
    15601 ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt tcgtcgcgat
    15661 ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg atacgttcta
    15721 cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca tcattaaagt
    15781 aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct tctgcgaatc
    15841 ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat ttaccttcta
    15901 catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc gtatttgggt
    15961 ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt ttgaatgata
    16021 caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct tcgtttcctt
    16081 ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt ttactcataa
    16141 ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt attgtgcaac
    16201 aatgaccatg gttggtctta acacgcgatc atgtaatttg tagccttctt gtagtacttc
    16261 tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg cttgatgaag
    16321 attaggatca aaggcttctc ctttagcagg gattttttca attccttctt cttccagtgc
    16381 gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc cttgatcatc
    16441 tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta attttttggc
    16501 taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc gatttgccat
    16561 atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta actcagcttt
    16621 taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat cttctgcagc
    16681 ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta gttcttcttg
    16741 attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga ttgctcttac
    16801 taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg agtttgacaa
    16861 gtcaagcgaa cggtagtaat cagcaagttt agctgctagt tctcttcgat aaacatcgac
    16921 tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta ttgtacctct
    16981 tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca ataaatcatt
    17041 gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga tcatttgtgt
    17101 aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa gatcttgacg
    17161 gggctcataa tccaagagat tcatttgtcc actgacataa accttatctt caaaaacatg
    17221 accaagaacg gtgtcaaata actgagacat cccttctgtc gtttggaaat atttatgaag
    17281 aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat ctccgactag
    17341 acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat attcgggaag
    17401 agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa taatctgtcg
    17461 attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt caggtcccaa
    17521 tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct taataatctc
    17581 attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat catcttctgc
    17641 tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag cagatggaat
    17701 ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg ctttcatatc
    17761 attgcgaatc gttgctgagc tggccttgac tccctcagac atcaatgttt tggaaccaac
    17821 gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt tttgtcgctt
    17881 tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta atccacttat
    17941 aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta gcactctaaa
    18001 cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaacg tttcaatagt
    18061 cctttattgt atttttgaac tttttttatt taaattcaga taaaaaacaa atattcttta
    18121 aactattttt caaacaaaaa gcgctcaaac acattgttcc ccaaaaaggt ccctcttggt
    18181 gtcaaataaa cacggtcttc ttcattaaca agtaaccctt cttgctctaa ttcccttagc
    18241 acatcaccgt acaccgattc aattgttttt tgatagcgat catagaaatg ttttttagta
    18301 atccctgttt ttttcttagt cctaagaaca tttcttcttc tatttgattt tttcgtgtca
    18361 atttttcttc ttccaatacc gggcgttcat ttgctcgtaa tgggcgtaaa tagtgttgga
    18421 ttggtccttt gttgcggtaa cgaatatctc caagataacc gctagcacca gctccaaacc
    18481 caaaatagtg gtcattgttc cagtacatga ggttatgttg agattcatgt ccggagagcc
    18541 caaagttact tatttcatat tggtgacgtc catgcttttc cattgcttca atcgtctctt
    18601 caaacattct ggtttccgtt tcctgatcag gcagttcgag tcttccttgt cgtacccagt
    18661 tcatgaacat tgttttgttt tcgaggatca atgagtacaa agaatagtga ggcagatcca
    18721 aagccaaagc tttttccaaa gtatccagat atccttctag tgtttgtccc ggtaatgcat
    18781 aaatcaaatc gatgcttaca ttggtaaaat tttctgcttc taaaaattgc atagtttgat
    18841 acacatcttc tgcggtgtgt ttacgtccaa tttttttcaa taagcgatta tcaaatgtct
    18901 gcactcccat ggataaacga ttgacgccat agtttttcat tacttgaagt ttttcacggg
    18961 tcaaatcacc tggattcgct tctacagtaa actcattacc attttgaaaa ggcaggatct
    19021 ctctagcacc tgataacagt cgatcaagtt gtttagcaga taaagaagtt ggtgtcccgc
    19081 cccctacgta aagtgtttct aaattcgctg tcggatgact ttctagcatt attttcattt
    19141 cattcagcag catttccaca tactcatcta ctggctgccc ttctaaaaaa actttattaa
    19201 aatcacagta atagcagata tgctcgcaaa atgggatatg aatgtacgca gaaaccttgt
    19261 tttctgcggg atttattgtt tctatcattg aaattctttc tccttacgtc gaaaagtcgt
    19321 gcctgttttc gaccttctta aaatcgcgtt caagcaccaa ctcattgaaa taagccgcat
    19381 atttagaatc aaggaaacct agctctaaaa atgcttgctt attgtcatcg gagctacacg
    19441 gtgcttttac agcctctcga taaacggtaa tcgcaaagct gaccttcggc attaagtcaa
    19501 aatctgcaaa aatatgaaaa gctattttca cagattcctc cttaagtgct cagaccacaa
    19561 cgctttgctt acaacctctc atatacgcat actaaacgta tttggcgaac aaatccacta
    19621 aaaaatccaa gaatcgactt cttggatgat tttttctttc attaaactgg ttacctagtt
    19681 atatatctct agggttgttt tcatgtctga gtgcccgata tgatttattt ttcgtttcta
    19741 taattacttt ttgccttcca gtttctttta atattgtact caagcaagtg ggaattggtt
    19801 cttttgtcat ccatgtatgc aaaatgttat aatgctggta aacaaaaatg attacctgtc
    19861 atttttattt agttcgtatt taattaagtt gactatctgt atactctgtc taaaacttaa
    19921 gaattgttgt tttcgctgtc gctacccaat tttaatgaat gtctgtaggt taaaactgtt
    19981 gtccttgtta tagatagtaa aggagagctt gaaaatgaaa actgcgatta cgtttctatt
    20041 agaggaatat gccgattggg aaggtgctta tattactagc acactaaacc aaaatgagaa
    20101 atggcaaaac aaaactgctt ctatccaatc agaggtcact tccattggag gattaaaaac
    20161 aaaaatcgat tacttaatcg aacaaatacc tgaaaaagtt gaccttttta ttatgatcgg
    20221 tggcaattca tgggacataa aaaatcatca tttatatcaa ataatcgata aattgttaaa
    20281 aacagacgta ccagtagctg ctatttgtgg ggcagttgat tatttagctc agagcggatt
    20341 attgcaaggt tacaaacaca ccggaaatgc gcaatttcct tggttagaat cggaaaatta
    20401 tcagaataaa gaagatttta tagaacaaca agctgtgaaa gatcgtaatt tgattactgc
    20461 gaatggaaca gctcctatag aattcacaga cttgattctt caagcagtcc attttgaaac
    20521 agaagaaata agagaaaaaa cgattcgtct atacaaactt ggattctatg attactataa
    20581 aaagtacgga gatcctttct cctaatcatt ctcttcaatt aaatactcct tatcaatagt
    20641 gtgacaagta attttactga tgattatttg tcacactttt tctgacattt atcaaagaag
    20701 atacactcta tttatattca tagacaaggt tttacggatt caaaaattca ggattatgct
    20761 atactggtat taaaaaaata atgaggtatt ttatatgaaa aagttaggtt ttattttatt
    20821 gttggtctct ttattccttg taggatgtac gtctcctagt aaagaaaata gtgatccatc
    20881 tgcgaccaca gacaaaagtt caatcagaca aaccagttca gctgaaaaat caagtactca
    20941 gaaatcagct tctactgaaa cgaatacatc ttcaacaagc agtaaagtct cagagcccaa
    21001 tacttcttct acatctagta gtacagttga gacaaatatt tcttcttcaa caatagacac
    21061 aacctcctca cagtcttctc agtcaataga tccgttagca ggatattctg atctacaaat
    21121 tgagtacgca cgtgtttggc tagctattag aggtaatact tttagagata catcgggtga
    21181 tttcgaatta catgttattc attatcctgc aggaacacct atcgcccctt atgatgcagg
    21241 gagtgcagtc tatccgaaag atgtggttat gctcactggg aaatacagtt accagggagt
    21301 tttagtgtat tcaagtaacc atgacgggac aatcactagt tatccagttc cttcacattg
    21361 gcaaatgcct gcagatcaaa catcttatcc agcgttcatt agagaaacaa ctcaagaaat
    21421 cattgataat gcatctgtag tagctattcc tacaggaaat cccaatgacg tgaaacagtt
    21481 aatcgatgtg acagttatag ataattagac tttctaatat gttatacaaa aagaagctga
    21541 cagcattttg tggctctata atttatggga ctgatgaatc agaat
//
LOCUS       DADQRX010000300        12226 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.079, whole genome shotgun sequence.
ACCESSION   DADQRX010000300 DADQRX010000000
VERSION     DADQRX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.079"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            243..518
                     /locus_tag="J8V14_003005"
     CDS             243..518
                     /locus_tag="J8V14_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0923020.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            531..2609
                     /locus_tag="J8V14_003006"
     CDS             531..2609
                     /locus_tag="J8V14_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBA0923021.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2769..4655
                     /locus_tag="J8V14_003007"
     CDS             2769..4655
                     /locus_tag="J8V14_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBA0923022.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4667..5614
                     /locus_tag="J8V14_003008"
     CDS             4667..5614
                     /locus_tag="J8V14_003008"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBA0923023.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5634..6161
                     /locus_tag="J8V14_003009"
     CDS             5634..6161
                     /locus_tag="J8V14_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBA0923024.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6224..6877)
                     /locus_tag="J8V14_003010"
     CDS             complement(6224..6877)
                     /locus_tag="J8V14_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBA0923025.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7009..7851
                     /locus_tag="J8V14_003011"
     CDS             7009..7851
                     /locus_tag="J8V14_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBA0923026.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8009..8905
                     /locus_tag="J8V14_003012"
     CDS             8009..8905
                     /locus_tag="J8V14_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBA0923027.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8908..9621
                     /locus_tag="J8V14_003013"
     CDS             8908..9621
                     /locus_tag="J8V14_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBA0923028.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9641..10159
                     /gene="msrA"
                     /locus_tag="J8V14_003014"
     CDS             9641..10159
                     /gene="msrA"
                     /locus_tag="J8V14_003014"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA0923029.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10156..10383
                     /locus_tag="J8V14_003015"
     CDS             10156..10383
                     /locus_tag="J8V14_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBA0923030.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10535..11086
                     /gene="lepB"
                     /locus_tag="J8V14_003016"
     CDS             10535..11086
                     /gene="lepB"
                     /locus_tag="J8V14_003016"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA0923031.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11169..11711)
                     /locus_tag="J8V14_003017"
     CDS             complement(11169..11711)
                     /locus_tag="J8V14_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBA0923032.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11948..12202)
                     /locus_tag="J8V14_003018"
     CDS             complement(11948..12202)
                     /locus_tag="J8V14_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBA0923033.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4257 a         2080 c         2462 g         3427 t
ORIGIN      
        1 ccattttttt gttgtgataa aagaacaaga aaggcaggaa tgggaaagta aacatccttt
       61 tgtcagaaac ttttatcttt cttaagtttt acttgatatc tgatactatt tggaatagaa
      121 tgaggcgttg aaaaatttat ggaaatttct aactagcaat aaacgatgaa acaatgaaat
      181 atttttcatc caaaaagaac tttgctataa tgatggaagt ttgtgtatag gagatctgga
      241 aaatgagaga gagttttgaa caacaaaaaa aactgttgca tgatcgctat ggcgcccttt
      301 cgatggatga ccgccgtcaa atactttgta agttgcgtaa aagaaatata ttgatgtatc
      361 gtcagttgga acgtctgaaa catgatctgc ttcgtttgga gtcaaaacgt gtacagtgtg
      421 aattagaggg gaaccaaaca caggttgaag tagtcgaaac caaaatttta aagaaaaaag
      481 aacaattttt aaaaatgctt acacaaaata aaaaataaag gggatggacg atggaatttg
      541 ctgagttagt tattgtattt gcagtaacga tcaccttatc gaacatagct agccgcattt
      601 tgccaatgat tccagctcca ctgatccaaa ttttccttgg agtggtatta ggtctgacag
      661 agtggggaca atcaatcgat tttgaacctg aattgttctt agttatgatt attgcaccat
      721 tgttatttag agaaggagaa aaagcagata tttcttcgat tctcaaaaat ttcgggacaa
      781 ttttgttctt ggcatttgga ggagtcattt tgacccttgt cggggtaggt gcgacgttat
      841 cattcctgtt gccaagtgtc ccacttgctg cctgctttgc ttttggagca gcgttaggtc
      901 cgacagatgc ggtagcagtc agttctcttt ctggacgagt gaatatccct aaaaaggcga
      961 tgcatatttt ggaaggagaa ggtcttttga atgatgcatc tggtgttact gccttccagt
     1021 ttgctcttgg tgcgctgatc accggatctt tttcagcagt aaacgcgggg atgagtctag
     1081 tagtttctag tattggggga gctttgatcg gctttttgct tgtctggttc aaacaaaaaa
     1141 taattcattt gatcgaaaaa gcatcagcac aagatgtcac agggtatctg ctgatcgaac
     1201 ttttgcttcc ttttctagcc tatgtattgg cagaggcatt tgatgtatct ggaatcattg
     1261 ctgcagtggc agcaggtatt ctccaagctt ctggttttag gaaaatctct gtgtttgatg
     1321 cagaattgtc gagcttgtcc cacagtacat ggacaactat tgcttttaca ctgaatgcat
     1381 tagtatttat atttttagga atcgaattga cgcaagtgtt ctcgccagta tggggagacg
     1441 gtttatatcc aaacggtctt ttattagcga ttatcgtatt aatctctgtc atgctttttg
     1501 tgatcagatt catttctatc agtctttttt atgtattcaa agatggcagt aaaaagttca
     1561 aaaaacagtt gaacgagatc ttgatcttaa catttggcgg tgtaaaggga acagtcagtt
     1621 tggctacgat ttttattttg ccattttcaa tcaataatat gatgttttac cagcggtctt
     1681 tgcttttgtt tctgacggcg ggtgtcatct tagtgacttt ggtcataggg atcattgtct
     1741 tgcctatgct gacggaaaca gaggaagcga aaagcacgga tttgaatgct ttgatgattt
     1801 tagaagaggt cgttgaagtt ttgcgaaaag aaataaagga aattgatcgt aatacaaaag
     1861 aatttttagc cactgaagca gtgatagaaa attatcaaga acggatacgt gatctgtact
     1921 tggaagattt aaccgatgat gaaaagcaag aagtacaaga aatacaagca ctgattttgt
     1981 cgatcgaacg ggatggattg gatgaaagtt atcgttcggg caagctttct tctaatggtt
     2041 atcgttttta ctcccgtttt ctttctcgat tcgaacattc tatcacaagc cagattttgt
     2101 cttttatcgg cttttggttc attgttgtcc gtaggttaat gcgaatcgtt ctccatccga
     2161 aaatgttttg gcaaagaaga tatgctgatc gtcagacatt tattagtgat gaagatatcc
     2221 aagaaatcag agagacatat caaaaaaata ctcagttgat tattgaaagt ttagataact
     2281 tgactgatat ttatgatgaa acgatggttc aattttttat ccagcaacgc aaacttgaag
     2341 gaataaaaat gaactcgggg aatttgattt cttcgtggat gatccagcaa gatagtctgt
     2401 ttacgaaaaa aatgctaaga ggttattatc ttgaaagaaa aatcattgat gaatacgaag
     2461 ttgctgaaaa aatcacgacg ttttctgcga acagctatcg caggaatatc aatttgttag
     2521 aatcttatac gatgaacaaa ccatctgata acttttcttt ccgatttgcg tttcgagcaa
     2581 agtcaaaaaa aacgagtcca ataaaataaa aaggtaaaaa ttttcaagtt ttataaaaaa
     2641 caaaacatat cagaacagga tcgaatcggt cctgttcttt ttttgagaac acaaaaagaa
     2701 caaataccaa aaaaattttc ctgaagttca ctatttttat gatacaatat ccaagataag
     2761 gagggaacat gttgaaagaa ttaaaagtaa ctgacctcaa aaaaacttat ggtgaaaaag
     2821 atttgtttga ccagatttct tttttggtcc acgaaaaaga ccgtatcggg ttgattggga
     2881 cgaacgggac gggtaaaaca agtctcttaa atattattgc cggtatcgat agcagcgacg
     2941 gtgatcggca aacagtgttt tatccaactg attaccgtat tggttatctt tctcaagcag
     3001 ctgaattttc cgatgaatta actgttctcc aagcagtctt tcaaggaaac agccccttga
     3061 tccaaactgt tcgagcatat gaggaagcat tgatcgaact aggggaaaac ggagaagacc
     3121 ctgatgtgca aaaacgttat gctaaagcag aagaacaaat gaataaagaa gatgcttgga
     3181 ctacagatac caacgcaaag attattttgc aaaaattagg aatcagtgaa ttagataaaa
     3241 aaatcagtac tttatcaggt ggtcagaaaa aacgagttag tctggcgcaa gtgttgattg
     3301 atgaacccga tttactatta ctagacgaac caacaaacca tttagattat gaagcgatcg
     3361 agtggttgga aaactatttg aaacaatacc gtggcgcact attgatggtt acccacgatc
     3421 gttattttct agatagagta gcaaaccgaa tcttcgaact ttcatttgga aaattatacg
     3481 agtataaagg aaattatgaa gcttacgtct tagaaaaagc agaacgtgat cgtgtagaag
     3541 tagaacaaga agaaaagcgc aagcggcttt ataaacagga attagcttgg atgagggctg
     3601 gcgtacaggc acgtggcacc aagcagcaag cacggatcaa ccgtttcgaa gatctaaagg
     3661 agaacttgta ccaagtaaac caagaagatg atttagagct gaacctagcg acacaacgtt
     3721 tagggaaaaa agtactggaa atcaaagatg gcagttaccg tataaatgac caaacactgc
     3781 tagaacattt agatttattg attcaatctc gggaaagact aggtattact ggtaaaaatg
     3841 gtgcgggaaa atcgacattg cttaatattc tagctggcag aattcccctg gatagcggta
     3901 caatgtctat cggtgaaacg gttcatcttg cgtattatac acaagaaaat gaagaaatgg
     3961 caccagataa acggatgatt gcttacttac aagaagctgc tgaagaagca aaaacagcag
     4021 atggcagtca aatcggagta gcagaattac ttgaacgatt tttatttcca agatttatgc
     4081 atgggacact gataggcaaa ctttcaggcg gagaaaaacg ccggttattt ttgttgaaac
     4141 tgctgattca gcaacctaat gttttattat tggatgaacc gaccaatgat ttggacatag
     4201 caacattgac gattttggaa gattatttta gaagttttcc aggtgctgtg atcactgttt
     4261 cccatgaccg ttatttcttg gacaaggtag cagataagtt attagtattc caaggaaacg
     4321 gaaaacaaga actttattac ggaaacatgt ccagttattt actgaagcaa aaagagaccc
     4381 agcagcctgc tgaaaaagcg aaaccaaaaa tacagagcaa ggaacctgct gaaaagaaga
     4441 aattatctta catggaacaa aaagaatggg aaacgattga agatgagatt gcagaacttg
     4501 aagaaaaaat ctcattgctc caagaagaga tgaatcatca aggagataat ttcactcgat
     4561 tacaagaatt gcaaaacgac gtatctgaaa cagaagcaca acttgaagaa aaaatggcac
     4621 ggtgggaata cttgagtgaa tgggtagaag attaggagga aaaaggatgg aacaagcgta
     4681 tcttgactta ggaagaaaat tgttggaaga agggcatcgt aaagaagaca gaacaggcac
     4741 aggcacatta agcttattcg gataccaaat gcggtttgat ctgcaaaaag ggtttccttt
     4801 gttaacaacg aaacgagtac cttttggatt gatcaaaagc gaattgcttt ggtttttaaa
     4861 aggagatacg aatatccgct atctattaca aaataaaaac catatttggg atgaatgggc
     4921 atttgaacgc tacatcaaaa gtccagacta taatggacca gatatgactg attttggcca
     4981 tagatgtttg acagatcctg aattcaatga agaatataaa aagcagagca agcttttttg
     5041 cgagaagatc ctaaccgatg attcctttgc tgaaacatac ggcgatcttg gacacatcta
     5101 cggctatcaa tggcgtcact gggaaacaaa agacggtggc tttatcgatc aaatcaaaca
     5161 agtgatcgaa gcaatcaaaa agactcctga ttctagaaga ttgatcgttt ctgcatggaa
     5221 tccggaagat gttcctagca tggctttgcc tccttgtcat acgatgtttc aattttatgt
     5281 tcaagaggga cggctaagct gccaattgta tcaacgcagt ggggatgtgt ttctaggcgt
     5341 tccgttcaac attgcaagtt atgcgttgct gactcaccta attgcacatg aaacaggttt
     5401 agcggttggc gaatttgttc acacattagg agatgctcat ctttatatca atcatatcga
     5461 acaaatgaaa gaacaattga gccgagaaat acgcgcattt ccgactttag ttttgaatca
     5521 ggaaaagcag tcagtatttg attttgagat ggaagatatc caattagaag gatatgaccc
     5581 acatccggcg atcaaagctc ctatcgctgt ttagaatcag aaaggatgag aaaatgttag
     5641 ctgcaatttg ggcacaggat gaaaacggat tgattggaaa agaagaccgt cttccttggc
     5701 gtttgccaaa tgatctgaaa ttcttcaaac aaatgacaga agcaaataca ctagtgatgg
     5761 gacgaaaaac attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag acgattgttt
     5821 tgacgagaga ttcttcctat aaagcagaag gcgtgcatgt catgcatagt gtggaagacg
     5881 tgcttgctct ggaaaaagaa acagacggta tctttttcat cgctggaggg tcagctgttt
     5941 atcaagaatt tctgccattt tgtacgatat tataccgtac agtcatccat catgtattcg
     6001 atggagatgc ttattttcca cctgttgact ggtctgattg gtcactgatc aatactagtc
     6061 aaggagaaat ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat cagcgtaatg
     6121 agaatcctgt aaaaagcggg acaggcgaat tgacgatata aaaaagctat ggatagacta
     6181 gctgttattt agagctgtcc catccatagc tttatttttt ttattaagta aagaataaaa
     6241 cagagaagta cataaaacct gcacctaaca taacaaacaa atgccaaaca acatgcatat
     6301 agcggataga attcaagcta taaaagaatg cgcctatcgt gtaagaaagc ccgcctgcta
     6361 ccatcaaaat aacaccagtc attccaagag agtgataaag aggaacagca gccacgacac
     6421 agagccaccc cataaaaata tagatgatcg tagatacttt tgatactctc tcccgtttat
     6481 gcaacgtcaa cgctttatat ataatgccaa gaatcgcaaa caaccagatt aatacaaata
     6541 aagtccaacc tagccatcct cggatactca gcaaacaaaa aggagtataa cttcctgcaa
     6601 tcaaaagaaa aatcgaacta tgatcgaata cttggaacac cttttttgct tttgtaaaaa
     6661 tcaatgaatg aaacaaagta gacgttaaaa ataaaagaat catcatcgat ccatagatag
     6721 cataagagac aatatggagg gaggaatgca agtgggcacc ttttactaaa agaaccacta
     6781 gtcccgcgat actcaatcca gtcccaattc catgggtgac tgcattaaac acttcattta
     6841 atatttcata cgttcgatta aatttcgttt tttccatgaa aacggctcct ttactaggtg
     6901 taattaatga aaaatcatta tttgtctgtt atactaaaaa tagaaattgt cttacatgta
     6961 gtttaacatg aataaggcat cttgttaaag aaagagagag agaaaataat gacaaatgta
     7021 aaaatcgtaa cagattcttc atgtacgatg gaaaaaaatg tacgcgatgc tttaggcatt
     7081 cgtgtcatgc cgctatccat catgatcgac ggaacagtat atcccgatga tgaccaacta
     7141 gacggcgaaa gatttatgga aatgatggcg aaagcaaaaa atctgcccaa aacaagccaa
     7201 ccaccaattg gagaattcat ctcgatgtat gatgagctag gagaagacgg cagtgaagtg
     7261 atttcgatcc atatgacaaa aggcttgagt ggaacagtag aagcagcaag acaagccagt
     7321 aatctaacaa aaacgaaagt aacggttatc gacagtgata ccactgacca aggattatct
     7381 tttcaggtaa ttcgagcagc tgaattagca aaagaaggta aaacagtaga gaaaatcctt
     7441 gaagaagtca aaaaagtaca agacaataca aaattataca tcggaatatc cacactagat
     7501 aacttggtaa aaggtggaag aatcagccga acaactggtt tgctgtctag tgttttgaac
     7561 atacgcgtgg tgatgaattt cgcccattct gaattaatcc ctgtaactaa ggggcgagga
     7621 aagaaaacgt tcgataaatg gtttaataat ttgaaaaatg aactgaaaga attgccaaat
     7681 gtgcgacaaa tcggtatttc acatgcggat gcaaaagaat tggcgcaaga attcaaagag
     7741 ggtttgcaag aattattccc ggacatgcat atccctgtac tgcatacgaa tccggttatc
     7801 gctacgcata ctggtaaaga cgcgtttgca atcatgtatt atgtagatta gtcgaaaaga
     7861 ggataagccc tcagcctgaa aggcttgcag cttattctct ttttctttat aaaattagga
     7921 aaataattgt tttctttttt cataaagact acctttttga taaaattttt gataacatta
     7981 gaaccaatcg aatacaagag gtgaaaaaat gaaattaacg aaacgacatt tgatcctcgt
     8041 tttgttccca ttattaattg cgatcatcat ctatgcaggt ttagctgctt ctataccgaa
     8101 agctggccca ctaatgaaac cagtaactac tgtgagagaa gatccaaata aaaaagaaaa
     8161 acttcactac atagctatcg gagattcgct gactgaagga gtaggagatc aaaccaagca
     8221 aggaggattc gttccccttg tcgcaaatga tctaaaagac cgttataatt tgaccgcggt
     8281 tgaaacagaa aactatggtg taaatggtga acgaagtgac cagatcttaa agcgagtaaa
     8341 aacaaaagaa gaaattcgaa acaatatcaa aacagctgat tttatcacac tgactgttgg
     8401 cggtaatgat ttgatgaaag ttatccaaaa taatttattc gggttaacgg tcaattcctt
     8461 taaaaagcca ctgaaaaact atcaggaaaa tgtcacagaa ctattggaag aatttcgatc
     8521 cctcaataaa accgcaccta tttacatttt aggcatctac aatccatttt atttgaactt
     8581 tcctgagatc actgacatgc agacaattgt taacaattgg aacaaaggaa cagaaacggt
     8641 gacaaaaaaa atgccgaact gctactttat cccaatcaat gatttgcttt atcaaggatt
     8701 gaatcaggaa gtaggtgtca cagaagaagg aacagatcaa acaactagca cgacagaaaa
     8761 tctaaatggc atcaaaaata atgtgctcta tgaagaagat catttccatc caaataattt
     8821 aggttatcaa ttgatggcaa atgcagtgag agatcagctg atcgagacac aaaaccaatg
     8881 gttagagaag gaggatgatc aataaacatg aaaacaagat ctgacgtatc ttcaaacaaa
     8941 acaaagaaaa caaattactg gaaattcgcc ttccttatcc ttcttggggt agtacttggt
     9001 tcgaccgtat ttttaggcag ccgtattttt gctaatcggg aaccagaact cccagaaata
     9061 ccagctctta cggaaagaca aggagatcct gtcttaacga tcaattcaaa taaagaaaaa
     9121 gtcaatcaaa tcatcagctt tttccttagt gagtatcaaa aagatagtga tattgaatat
     9181 aaattttatt tggaaaatga agcactactc aatggtactt tcgaagtact aggttttcct
     9241 atcgatttct atttatattt tgatccgtat gtgatggaaa acggaaatgt ccaactaaaa
     9301 gctagaagtc tttctatcgg aacattgagt ttgccaatca aagatgtgat gaatatgatc
     9361 aagagaaact ataaattacc agattggatc gaagtcaata cagatgatct gactatcatg
     9421 cttaggttgg atcaattccg aatgcaaaac gggatgtata ttaaagcaga taaaatcgac
     9481 ttagtaaatg atgacattcg gtttagtcta tatttgccaa aagatacctc tgatgcttca
     9541 aacaactcaa ataccaacaa aactagcacc agcagtactg aaagcaagac aaaagaaaat
     9601 aaaaaatcat cggatgagta gtattagagg gggaatcata atggaacgag caatatttgc
     9661 aggaggctgc ttctggtgta tgatccagcc tttcgacacg cttcctggta tccatacagt
     9721 gatgtcagga tatactggag ggcatgtacc agatccgact tatgaacaag taaaaagcca
     9781 aacgactgga catacagaag cagtggagat tatctatgat ccaacattga tttcatatga
     9841 acaattagta gaactttact ggcagcaaac tgatccaact gacgctttcg gacaattcga
     9901 ggatcgcggt gacaattatc gcccggtgat cttttatttg acagaagaac agcggatcat
     9961 tgctgaaaaa agtaagaaac agttagctga aagtggacgt ttcgaacagc caatcgttac
    10021 aaaaatcgaa ccagcccaac cattttatct agcagaagaa gaccatcaag agtattataa
    10081 aaagcatcct gaaaatttta aaagaaacca tgcacgccgg gctgccttta tcgaagaaaa
    10141 ttggaggaac gaagggtgaa tcgaagcttt tttcattatt taatgacact gcgaggcggt
    10201 ggcacacatg atgctttatc tgttttcgct acagaagccg gtaaagacat ccaatttcct
    10261 aagcacagct ctagctacga agaaatttct aattatctgg aactgaatgt cgactatttg
    10321 ccgtccatgg atatctttga cgaagcatgg gaaaaatatt tagaaaataa tcatgacata
    10381 taagagatag ttttgtactt tatgatcaaa aaagtttttt gtccaatagc ctaattgaaa
    10441 gaattatttt tatttcagct atctagtcgc atttttttga tatttttctt ataataaaga
    10501 aagaatgaaa aaaagaggga tggattgata gagattgaca aaaaaacaac gctatattaa
    10561 tcgcttctgg cttgtattca aatacttact tgtttctgta ttccttgcgt tcatgttgcg
    10621 gggattttta ttcatcccgg taccagtgga aggcaattca atggaaaacg tgttgaagca
    10681 aggtgatatg gttgtgatgg aaaaattttc ggagatcaga cgatttgata ttgttgtttt
    10741 tcaattagca gatggcacga tttatatcaa acgagtaatc ggtcttccag gagaaaatgt
    10801 cagctaccaa aatgatcaat tgaagatcaa tgggaaagta gttaaggaac cttatctaac
    10861 taaaaacctg aaatcagatc atgcaaatgc atcctatacg acggacttta cactacaaga
    10921 gctaacggga caatccaagc ttccggaaga taattatttt gtacttggag ataatcgaag
    10981 agtttcaaaa gatagtcgtt cgtttggcac gatcaataaa actgatattc taggtaaagc
    11041 aagatttgtc tattatccac ttgatgaaat aaaatggatc cagtgagaaa caataaagcg
    11101 tgcagtctgt tgagactgca cgctttattg tttaatccaa aatcagttta ctaccttagt
    11161 acgtaaaatc aaacagctcg tttaaacact tttctagcct ctaaagtttt gataaatgaa
    11221 taaacaacca ttatttgaat agggatttgg atcatattcc tgatgaatct acctagaaaa
    11281 attactttga aatctgcacc aaacaaaaca gacatcagcc aagaattgat aaaaaggctg
    11341 actgggatca taatcaataa ttgggcagcg atgacggaag gcaggtttat ctttttatga
    11401 tataaaaata agccattaat aaatccacca aggaaagccg tgataagaaa agcagcttga
    11461 aacgttcctg aaccaaatat cgtgaaattt aaagtgttcg acaacatttc aagcaaacca
    11521 gcccagatcg gtccatatag ataacccata agcgccatac ttaaaaatcc gaatgtgatt
    11581 tgaaaataag caggaccaat cgctatcttg tataagatga catcaagggc taataaaaga
    11641 gcagaggtcg acaacaatct tgcattcatt tcgggcaatc taaaaacgtc ttttgcaatc
    11701 aaagtgttca aaatattctc tcctttgtca gttaataata aaaggatacc ataaaatcac
    11761 caatgtgtca tgacacattg gtgatttaca tacactggca tctaacaatt aaaaaaaggg
    11821 tgagaagact gcttttataa actgtaaaaa agagattgta cgaataaaaa aaatagaggc
    11881 ggagaatact tatcgtatca tccgcctttg tttttttgtt ataaaaaacg tcagattagt
    11941 tatctgctta catcttcatc catacgggtt ctaatgtatt tccgtctaga tcttggactt
    12001 ctaatgaaaa catttcttct tcaggcattc ccatatctac ttcgtggaaa gtaccaccat
    12061 tttcttttgc aatttgagca aattttttta cttcatccgc attttcaaca ctaaacgaaa
    12121 tcaaagcacc acttgttttt ttattatcag ctatttcttt atcttttata aattttttat
    12181 aaaaatcatg agagagaagc attatccaaa aagtgtcatc ccatac
//
LOCUS       DADQRX010000301        10815 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.091, whole genome shotgun sequence.
ACCESSION   DADQRX010000301 DADQRX010000000
VERSION     DADQRX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.091"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(204..2162)
                     /locus_tag="J8V14_003019"
     CDS             complement(204..2162)
                     /locus_tag="J8V14_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0923034.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="J8V14_003020"
     CDS             complement(2159..3031)
                     /locus_tag="J8V14_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA0923035.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="J8V14_003021"
     CDS             complement(3373..4245)
                     /locus_tag="J8V14_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA0923036.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="J8V14_003022"
     CDS             4373..4942
                     /locus_tag="J8V14_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBA0923037.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="J8V14_003023"
     CDS             5009..5746
                     /locus_tag="J8V14_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA0923038.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="J8V14_003024"
     CDS             5977..7245
                     /locus_tag="J8V14_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HBA0923039.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="J8V14_003025"
     CDS             7732..8196
                     /locus_tag="J8V14_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA0923040.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="J8V14_003026"
     CDS             8180..8809
                     /locus_tag="J8V14_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBA0923041.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr"
                     /locus_tag="J8V14_003027"
     CDS             9163..10641
                     /gene="msr"
                     /locus_tag="J8V14_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HBA0923042.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..>10815
                     /locus_tag="J8V14_003028"
     CDS             10688..>10815
                     /locus_tag="J8V14_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBA0923043.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGL"
BASE COUNT         3490 a         2009 c         2178 g         3138 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtct
//
LOCUS       DADQRX010000302         9305 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.105, whole genome shotgun sequence.
ACCESSION   DADQRX010000302 DADQRX010000000
VERSION     DADQRX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.105"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(157..1098)
                     /locus_tag="J8V14_003029"
     CDS             complement(157..1098)
                     /locus_tag="J8V14_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBA0923044.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1224..2345
                     /locus_tag="J8V14_003030"
     CDS             1224..2345
                     /locus_tag="J8V14_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBA0923045.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2603..4105
                     /locus_tag="J8V14_003031"
     CDS             2603..4105
                     /locus_tag="J8V14_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBA0923046.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4172..4699
                     /locus_tag="J8V14_003032"
     CDS             4172..4699
                     /locus_tag="J8V14_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA0923047.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4910..5971
                     /locus_tag="J8V14_003033"
     CDS             4910..5971
                     /locus_tag="J8V14_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBA0923048.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5974..6870
                     /gene="murQ"
                     /locus_tag="J8V14_003034"
     CDS             5974..6870
                     /gene="murQ"
                     /locus_tag="J8V14_003034"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBA0923049.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6884..8866
                     /locus_tag="J8V14_003035"
     CDS             6884..8866
                     /locus_tag="J8V14_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA0923050.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8931..>9305)
                     /locus_tag="J8V14_003036"
     CDS             complement(8931..>9305)
                     /locus_tag="J8V14_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBA0923051.1"
                     /translation="KMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGL
                     DNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLAD
                     YELRGTPSDVTRIYQILAENNK"
BASE COUNT         3035 a         1661 c         2036 g         2573 t
ORIGIN      
        1 caaataaaaa acctacattt tgaagagatt atgtactgaa acaaacaagc acaattctta
       61 tggcatcatg agagttgtgt tttctttttg atcttttatt gggcttaaaa aaagagaacc
      121 agctgctgat tcagctgatt ctcatcagag agccttttat tctgcgccaa ggattccttc
      181 ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat catatttttg
      241 tccttttcgc caaatggcac cattgatgta gtcgatttca gtcaaacgat ggtttttgat
      301 caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc
      361 aatatgttgg tacacttctt cttgatctag tatgattcct tcttttgctg cgatagaagc
      421 aaattcagat acgattgttc gaaccatgct ctctgccgct ttttgcgcgc ctagttcagc
      481 aatattacaa tccaaaatcg tgcataaacc attcaatgtt ccatttacac aagcttttcg
      541 ccaaatggaa taacgaacgt tatggctata gatcggattt aaatttgctt cttgaaagac
      601 ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta gttcgatttc
      661 tccatcacct aacaatgtga cttttccggg accagctaga ccagctgtcc acatcgtgat
      721 cccaaataag atatttttct ttggcacata tttttccagt acatcttcat gacctaatcc
      781 atttagtaaa cacaaaacat aagtattgtc agtaataatg gattggatcg aacaaaacat
      841 atcatctaat tgattggctt ttgttaatgc tacgatcaag tccacgtgtt cgttgctttc
      901 gatgatttct tctggtgaat agattggcag ttttgcgacc acttctttac cgttaaagtc
      961 agcaatcaac ccatttgtac gaatggcttc aatgtgagca ggccaacgat cgatcaagag
     1021 gacctcattt ccgctttgat gtagcatcaa tccgattcgg ctgcccattg ctccggcccc
     1081 agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct tttagtatat
     1141 agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa gtgtcttact
     1201 taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca tttaaaggaa
     1261 gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat cgtaaaggaa
     1321 gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca atctattacg
     1381 cgctcagggg aacatggcgt accattgatg ggatggatct cttattccgt cagcgaaaaa
     1441 tcagctattt attgacaact tggcaaggaa aaaacaccgc agtcatcgaa tctgcacaag
     1501 cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt gcttccagtt
     1561 atgccttagg agaacttatt aaagatgcac tgcttcaaga agtagaacaa attattgtca
     1621 ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc ttgggtgctt
     1681 ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt cattttgatc
     1741 gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt accaatccat
     1801 atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga atagatacga
     1861 cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa gatttctggg
     1921 aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt ggagcccttt
     1981 ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc actggattgg
     2041 aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc gatcagcaaa
     2101 cgatccacgg aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa
     2161 taatcgctat ttgcggaagt agagaccaag agctagaagg actttctgat tttctcccaa
     2221 ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa cacaggcaaa
     2281 cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc atcctcccga
     2341 agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg acaaagtgag
     2401 ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt
     2461 ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg atgtatgggt
     2521 ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat aggactggtc
     2581 tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga cattcggcta
     2641 cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca cacaatggaa
     2701 actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata ttctacaaaa
     2761 caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca
     2821 gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg attttgagat
     2881 atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct tatggagaga
     2941 attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt tatttgtgga
     3001 tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggtagaaa
     3061 acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg
     3121 gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc aactggaact
     3181 ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat
     3241 ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa
     3301 agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg gattcatcga
     3361 tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac ggacgatgaa
     3421 acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag aaaaactatt
     3481 aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc accataagcg
     3541 acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg agccaattca
     3601 ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg caggaaagtc
     3661 atccattatc cattatcttc tgggggcatt caacggcaag gttataggag aaaaatacca
     3721 gccaaaacat ctgagcatta gttatgcaag ccaaaattat gaagacaatc gaggaacgtt
     3781 ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca acctccgaaa
     3841 gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta tgggccaacg
     3901 gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata catgggatga
     3961 accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga tcttgaacgt
     4021 gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag tatctactga
     4081 gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag gtatggttaa
     4141 ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac acggagaaac
     4201 aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg
     4261 acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa
     4321 aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg ctgatcgtct
     4381 cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg attatgatgg
     4441 actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg tacgattccc
     4501 aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga aagaatgcat
     4561 cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa gagtgatcaa
     4621 tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag ataatacaga
     4681 attggtttct tatgagtaat tatgtttttg tgttatttaa ataaaatatt tcaaggcact
     4741 gcttttgctt agcagtgcct tttttaggtt atgacaattc ttttgataat ataaactcaa
     4801 cctttagaaa tgaaatttaa ttattttaat aaaagtattg aaattttgtt tctttagata
     4861 tatactctag tcatagaaat gaagacggat acagaaaaag agggataata tgtttggctt
     4921 ttcaatattt atgaacgatt ctttgtcaga agagaagaaa cagtatatcc gccaaatggc
     4981 atccaatggc tttgtcggta ttttcacttc catgcatata ccagaagatg atgtttcggc
     5041 atatcaaaag cgattgatcg aactaggaag cgaagcgaaa aagaataaac tagaattgat
     5101 ggtcgatatt tcaggagagg cattggaacg agcgggtttt tcattagaag atctaacgcc
     5161 tttgaaagca attggtgtaa caggtttaag aatggattat catatttcca atcagcagat
     5221 tgccgactgg tcacatcaat tgaaaatcag tttgaatgct agcacgatca cgcctaaaga
     5281 tatcgatgaa ttgaaagaag cagaagctga tttctcacag attgaagctt ggcataatta
     5341 ttatccacgt cccgaaacag ggctggacaa agaatggtat cagaaaaaaa atcaatggct
     5401 caaaattcaa ggtctccttg tccaaggttt tgtaccagga gatacagagt tgagaggccc
     5461 actatatcaa ggattgccaa ctttggaaga acatcgaggg atccatcctc ttgcaggcgc
     5521 tctagatcta ttagcaagta acacggatat tgtttacatc ggagatgcag gtctgtcaga
     5581 aaacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc tccatacgga
     5641 acctgtagat gaagaatttt acgaatatat attagggaag catacgaatc ggcaagacga
     5701 tgcaagagat gtcatacgaa gcgtagatgc acgttttcga gagattccgc ctatcccagc
     5761 aagaaataca gctacgcgta tgaaagggtc tattacttta gataacgaaa agtatttgcg
     5821 ctatatgggt gagatccaat taaccaaata tgatttgcca gcggatgaaa aagtgaatgt
     5881 agtagctaaa gttatcaaag aagagctacc attgatcaac caaatcaaag ctggtatgaa
     5941 ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt agagaaccta
     6001 acaacggaac gcagaaacca agaaactttt ggattagatg agatgagtat ttctcaagcc
     6061 gttcaactaa tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa agaactgaca
     6121 agtatcgaac cagtgattgc taacacgatt gagtccttta aaaaaggtgg acgtctgatt
     6181 tatatgggag ccggaacaag tggaagacta ggtgtgttag atgctgctga atgcgtaccg
     6241 acattcggtg tagaaccgga aatggtcata ggtctaattg ctggtggaga agaagcaatg
     6301 actgtagctg tcgaaggggc cgaagattct tatgagctag ggcaagaaga tctaatcaag
     6361 ctgaatttga caaaaaatga tatagttatt ggtatcgcag ctagcggtcg tacaccatat
     6421 gtgatcggtg gattagatta cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc
     6481 aataaacaag ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca
     6541 gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact tatcttgaat
     6601 atgatttcta ctatcagtat gattggtata ggtaaagtat tcaataactt gatggtcgat
     6661 gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca
     6721 gaagctgatt acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg
     6781 gctatcgtca tgattttgac aaatagtgat aaagaggaag ctcaagcaaa attagctgaa
     6841 gcaaacggat ttgtaaaagg gaccttatga gggggaaaag accatggcaa aaaacgaact
     6901 gactttggaa gaactggcaa aacaaattta taaaggggca ggcggcatgg gcaatgtaga
     6961 aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa
     7021 tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta gtagaagatg aacagcttca
     7081 agtcatcatc ggacctggga aagtgaacaa agtagcacag caaatggttg atttggcagg
     7141 cgtgaaatta ggagaaaaac tttcttctgt aagtacagct acagcaaatt catcagcatc
     7201 tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca
     7261 aaaaccatct aaaatcaaat cggtattaaa agacatttct aatatctttg tgccaatgat
     7321 tcctgcattt gtcggtactg ggatcgtggc agggattgcc gcagttttag caaacttagt
     7381 cacagccgga acacttgatg cagcaacatg gcaacaatat atcgatgtga tgaacatctt
     7441 gaaaaacggg atgtttagct acctagttat ctatacaggt atcaatgctg cacaagtttt
     7501 tggtgcaaca ccaactcttg gtggagttat cggggccgtt gtgatgctga cagggatgaa
     7561 tccggaagca ccactgaaaa acttatttac tggtgaagct cttgcagctg gacaaggtgg
     7621 gatcctaggt gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa aactgcataa
     7681 agtagtacct gatgcggtag atatcatcgt tacaccaact ctttcattga ttgccatcgg
     7741 tctgattgaa attttcttca tcatgccttt agcaggtttc atttctgatg gcatggtcgg
     7801 tgggatcaac tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc taggcacatt
     7861 attcttgccg atggttatgt tcggactgca tcaaattttg actccaatcc atgtgcaaat
     7921 gattgatgaa actggtcgta cgctattgct tccaattctt gcgatggctg gtgcaggaca
     7981 agtgggtgca gcattggcat tatggattcg ctgcaaaaaa gataaagaat taacagaaat
     8041 gatcaaagga gctttaccag tcgggatcct aggaatcggc gaaccattga tttacggagt
     8101 aacattacct ctaggacgcc catttattac tgcttgtatc ggtggaggta tcggcggggc
     8161 agtgatcgga atgatcggaa atgtcggggc tatcgctatt ggaccttcag gagccgcttt
     8221 gatcccattg atctctgacg gtaaatggtt aggctacatc cttggattat tggcagctta
     8281 tgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt tagaaaaaga
     8341 agaattagcg actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac
     8401 ttcagctgaa agtgtgacta tcctctcatc agttgctgac ggaacagttg tttctttaga
     8461 taaagcaagt gatccagttt ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc
     8521 agaaaacgga catatctatt ctccagttac tggggtagtc agctctgtct tcccaacgaa
     8581 acacgctatt gggatcacaa cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa
     8641 cacagttgac ttaggcggta cgccatttga tgtgaaagta acagaagggc agcaagtgac
     8701 acctgatacg cttgtagcag aagctgactt agaagcgatc aaagcagcag gcaaagaaac
     8761 atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac
     8821 aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa
     8881 tagaaacgaa agaagaaact gaaatctatt tttcagcttc ttctttttta ttatttattg
     8941 ttttctgcta aaatctgata aattcgtgtc acatcagatg gtgttccccg taattcataa
     9001 tctgccaaga aaggaaagcc aaatgtttgt gcatattgtt tggctgttag acaatactga
     9061 ttattgaagt ttttatttcc gctaccgact acaccaagac aaagtttatg attgtcttca
     9121 tgttccagat attcacgcat cgtttcggtc aagatttctg tatcaccgtt atctaaacca
     9181 tttccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa
     9241 tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc ttctgcatgc
     9301 atttt
//
LOCUS       DADQRX010000303         8456 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.111, whole genome shotgun sequence.
ACCESSION   DADQRX010000303 DADQRX010000000
VERSION     DADQRX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.111"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            259..1170
                     /locus_tag="J8V14_003037"
     CDS             259..1170
                     /locus_tag="J8V14_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBA0923052.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1323..1808
                     /gene="vanZ"
                     /locus_tag="J8V14_003038"
     CDS             1323..1808
                     /gene="vanZ"
                     /locus_tag="J8V14_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBA0923053.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2327..2611
                     /locus_tag="J8V14_003039"
     CDS             2327..2611
                     /locus_tag="J8V14_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0923054.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2977..3276
                     /locus_tag="J8V14_003040"
     CDS             2977..3276
                     /locus_tag="J8V14_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA0923055.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3319..4809
                     /locus_tag="J8V14_003041"
     CDS             3319..4809
                     /locus_tag="J8V14_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HBA0923056.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5341..5958
                     /locus_tag="J8V14_003042"
     CDS             5341..5958
                     /locus_tag="J8V14_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA0923057.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5958..6896
                     /locus_tag="J8V14_003043"
                     /pseudo
     CDS             5958..6896
                     /locus_tag="J8V14_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(7589..8236)
                     /locus_tag="J8V14_003044"
     CDS             complement(7589..8236)
                     /locus_tag="J8V14_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HBA0923058.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         3099 a         1268 c         1490 g         2599 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
     1201 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
     1261 gttaactagg acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta
     1321 tattgggaaa aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt
     1381 tagtgttatt caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc
     1441 ttaacttgac tccatttact gctactggga atttcagaga gatgatagat aatgttataa
     1501 tctttattcc atttggcttg cttttgaatg tcaattttaa agaaatcgga tttttaccta
     1561 agtttgcttt tgtactggtt ttaagtctta cttttgaaat aattcaattt atcttcgcta
     1621 ttggagcgac agacataaca gatgtaatta caaatactgt tggaggcttt cttggactga
     1681 aattatatgg tttaagcaat aagcatatga atcaaaaaaa attagacaga gttattattt
     1741 ttgtaggtat acttttgctc gtattattgc tcgtttaccg tacccattta agaataaatt
     1801 acgtgtaaga tgtctaaatc aagcaatctg atctttcata cacataaaga tattgaatga
     1861 attggattag atggaaaacg ggatgtgggg aaactcgccc gtaggtgtga agtgagggga
     1921 aaaccggtga taaagtaaaa agcttaccta acactatagt aacaaagaaa gcccaattat
     1981 caattttagt gctgaggaat tggtctcttt aataaatttc cttaacgttg taaatccgca
     2041 ttttcctgac ggtaccccca ctagcagcca tgcttttttt gaatctttgt aggacgtttt
     2101 aaagagaatt acccacttta aaattaaaca tgcgcttaga atcgctttag gaaacacgat
     2161 ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt ggtgaaatta cctacttttg
     2221 gaataatcac aaatcacaag tgattaatca cttgtttatt aagatattaa aagctataat
     2281 ttaaataaag cgtgaatttt attacacaaa aagagggggg agaaacttgg aactagcatt
     2341 tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa gaaaaattct cccaagaatt
     2401 agaaatgagt agatcaaatt attcacgaat agaatcagga aaatcagatc caaccataaa
     2461 aacactagaa caaattgcaa agttaactaa ctcaacgcta gtagtggatt taatcccaaa
     2521 tgagccaaca gaaccagaac cagaaacaga atcagaacaa gtaacattgg atttagaaat
     2581 ggaagaagaa aaaagcaatg acttcgtgtg aataatgcac gaaatcgttg cttatttttt
     2641 ttaaaagcgg tatactagat ataacgaaac aacgaactga atagaaacga aaaaagagcc
     2701 atgacacatt tataaaatgt ttgacgacat tttataaatg catagcccga taagattgcc
     2761 aaaccaacgc ttatcagtta gtcagatgaa ctcttccctc gtaagaagtt atttaattaa
     2821 ctttgtttga agacggtata taaccgtact atcattatat agggaaatca gagagttttc
     2881 aagtatctaa gctactgaat ttaagaattg ttaagcaatc aatcggaaat cgtttgattg
     2941 ctttttttgt attcatttat agaaggtgga gtttgtatga atcatgatga atgtaaaact
     3001 tatataaaaa atagtttatt ggagataaga aaattagcaa atatctatac actagaaacg
     3061 tttaagaaag agttagaaaa gagaaatatc tacttagaaa caaaatcaga taagtatttt
     3121 tcttcggagg gggaagatta tatatataag ttaatagaaa ataacaaaat aatttattcg
     3181 attagtggaa aaaaattgac ttataaagga aaaaaatctt tttcaaaaca tgcaatattg
     3241 aaacagttga atgaaaaagc aaaccaagtt aattaaacaa cctattttat aggatttata
     3301 ggaaaggaga acagctgaat gaatatccct tttgttgtag aaactgtgct tcatgacggc
     3361 ttgttaaagt acaaatttaa aaatagtaaa attcgctcaa tcactaccaa gccaggtaaa
     3421 agcaaagggg ctatttttgc gtatcgctca aaatcaagca tgattggcgg tcgtggtgtt
     3481 gttctgactt ccgaggaagc gattcaagaa aatcaagata catttacaca ttggacaccc
     3541 aacgtttatc gttatggaac gtatgcagac gaaaaccgtt catacacgaa aggacattct
     3601 gaaaacaatt taagacaaat caataccttc tttattgatt ttgatattca cacggcaaaa
     3661 gaaactattt cagcaagcga tattttaaca accgctattg atttaggttt tatgcctact
     3721 atgattatca aatctgataa aggttatcaa gcatattttg ttttagaaac gccagtctat
     3781 gtgacttcaa aatcagaatt taaatctgtc aaagcagcca aaataatttc gcaaaatatc
     3841 cgagaatatt ttggaaagtc tttgccagtt gatctaacgt gtaatcattt tggtattgct
     3901 cgcataccaa gaacggacaa tgtagaattt tttgatccta attaccgtta ttctttcaaa
     3961 gaatggcaag attggtcttt caaacaaaca gataataagg gctttactcg ttcaagtcta
     4021 acggttttaa gcggtacaga aggcaaaaaa caagtagatg aaccctggtt taatctctta
     4081 ttgcacgaaa cgaaattttc aggagaaaag ggtttaatag ggcgtaataa cgtcatgttt
     4141 accctctctt tagcctactt tagttcaggc tattcaatcg aaacgtgcga atataatatg
     4201 tttgagttta ataatcgatt agatcaaccc ttagaagaaa aagaagtaat caaaattgtt
     4261 agaagtgcct attcagaaaa ctatcaaggg gctaataggg aatacattac cattctttgc
     4321 aaagcttggg tatcaagtga tttaaccagt aaagatttat ttgtccgtca agggtggttt
     4381 aaattcaaga aaaaaagaag cgaacgtcaa cgtgttcatt tgtcagaatg gaaagaagat
     4441 ttaatggctt atattagcga aaaaagcgat gtatacaagc cttatttagt gacgaccaaa
     4501 aaagagatta gagaagtgct aggcattcct gaacggacat tagataaatt gctgaaggta
     4561 ctgaaggcga atcaggaaat tttctttaag attaaaccag gaagaaatgg tggcattcaa
     4621 cttgctagtg ttaaatcatt gttgctatcg atcattaaag taaaaaaaga agaaaaagaa
     4681 agctatataa aggcgctgac aaattctttt gacttagagc atacattcat tcaagagact
     4741 ttaaacaagc tagcagaacg ccctaaaacg gacacacaac tcgatttgtt tagctatgat
     4801 acaggctgaa aataaaaccc gcactatgcc attacattta tatctatgat acgtgtttgt
     4861 tttttctttg ctgtttagcg aatgattagc agaaatatac agagtaagat tttaattaat
     4921 tattaggggg agaaggagag agtagcccga aaacttttag ttggcttgga ctgaacgaag
     4981 tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca cttgattttt
     5041 taattttcta tcttttatag gtcattagag tatacctatt tgtcctaata tgattttagc
     5101 agtataattg acttggtgaa taggtcattt aagttgggca taataggagg agtaaaatga
     5161 aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt aatgagcaac
     5221 aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa tttgaaattg
     5281 aaaaagtaga tattgctgat tggttattgc aaccgagaga attttagagg ttggttgaaa
     5341 atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga tcggcaatta
     5401 caagcgttac agggcgtttc taaggtcttt tcagacaaat taagcggtca atcggtcgaa
     5461 cgcccacaat tacaagctat gcttaactat attcgtgaag gggatattgt tattgttact
     5521 gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa tcaaattcaa
     5581 attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat tgaagatgaa
     5641 aatttaaggc gtttgattaa tagccttgtc attgaattgt acaagtatca agcagaatca
     5701 gaacgaaaaa aaattaagga acgtcaggca caaggaatcg aaattgctaa gaaaaaaggc
     5761 aaattcaaag gtcgtcagca taaatttaaa gaaaatgatc cacgtttaaa gcatgctttc
     5821 gatttgtttt tgaatggttt atccgataaa gaagttgaag aacaaactgg aatcaatcgc
     5881 cgaacgttta gaaggtatcg agcaagatat aacgtgacag tcgatcaaag aaaaaacaat
     5941 gaaaagaggg atagttaatg agtacggtta ttttagctga aaaaccaagc caggcattag
     6001 cctacgcaag tgctttaaaa caaagcacca aaaaagacgg ttattttgag atcaaagacc
     6061 cactatttac agatgaaacg tttatcacct ttggttttgg gcatttagtg gaattagcag
     6121 aaccaggtca ttatgacgaa aagtggcaaa attggaaact tgaatctttg ccgatttttc
     6181 ctgatcgata cgattttgaa gttgcaaaag ataagggaaa gcagtttaaa attgttgcag
     6241 aacttctcaa aaaggcaaat acaattattg ttgcaacaga tagcgacaga gaaggtgaaa
     6301 atatcgcctg gtcgattatc cataaagcaa atgccttttc aaaagataaa acatttaaaa
     6361 gactatggat caatagctta gaaaaagatg taatccgaag cggttttcaa aatttgcaac
     6421 ctggaatgaa ttactatccc ttttatcaag aagcgcaaac acgccaaatt gccgattggt
     6481 tgatcggcat gaacgcaagc cctttgtata cgttaaattt acaacagaag ggcgtacaag
     6541 gtacattttc actaggacgt gttcaaacgc ccaccttata ccttattttt cagcgccagg
     6601 aagccataga gaattttaaa aaagaacctt ttttcgaggt ggaagctagt ataaaagtaa
     6661 accaagggtc gtttaagggc gttctaagcc ccacacagcg ttttaaaacc caagaggagc
     6721 ttttagcttt tgtttcttct aaacaagcta aaataggcaa tcaagagggg ataattgctg
     6781 atgttcaaac caaagagaag aaaacgaata gtccgagttt gttttcttta agtagtttgc
     6841 aatcaaaagt caatcagctt tataaagcga cagcgagcca aactttaaaa gctatttctt
     6901 ttttaataac ttaaaaataa acttaatgta acagcaagca cagtcaaggt atacaccttt
     6961 gacaaaaaat agcacattct ctatcgaaaa tttttgctta ttttttaaat tattttggga
     7021 aattttccca atcccttttt ctaactcaaa aaatataatc actcaaaatt taaaagggcg
     7081 cacttataca tcattttaaa aaattgatgt aacgtgctaa gttcaaaaca aagggcgcac
     7141 ttatacacga ttttcaatct tgtatatttc taacgaaaag cgtgcgccaa aaaaccccct
     7201 tcgtcaattt tgacaggggg ctttttgatg taaaaatttc tatcgaaatt taaaaattcg
     7261 cttcactcat gttataaaga cttaaaataa aataactctt taaaatcttt tgctagttgt
     7321 tcttcaatat tttttattcg gtgcatcttc caagtaaagt ataacacact agacttattt
     7381 actacgtttc ataagtcatt aatgcgtgtg ctctgcgagg ctagtttttg tgcaagcaca
     7441 aaaaatggac tgaataaatc agtccataag ttcaaaacca aattcaaaat caaaaccaca
     7501 agcaaccaaa aaatgtggtt gttatacgtt cataaatttt atgatcactt acgtgtataa
     7561 aattaaattc actttcaaaa tctaaaaact aaatccaatc atctacccta tgaattatat
     7621 cttgaaattc attcataaat agtgaagcat ggtaaccatc acatacagaa tgatgaagtt
     7681 gcagagcaac tggtatataa attttattat tctcactata aaatttacct atcgtaataa
     7741 taggcaataa aaagctgcta ttgttaccaa tatttaaatt aaatgaacta aaatcaatcc
     7801 aaggaatcat tgaaatcggt atggtgtttt caggtatcgg ttttttagga aacatttctt
     7861 ctttatcttt atattcaagc aagtcatttt tataattatt ataaaaagaa atgaagtttt
     7921 tatcagattc agtccaaatg ttagtaaatt tttcagtttg cttattaaaa actgtataca
     7981 aaggatttaa cttatcccaa taacctaatt tattctcact attaattcct gttctaaaca
     8041 ctttattttt atttacaact tccataattg cataaattaa agagggataa atttcatatc
     8101 ctttcttttt tatcatatct ttaaacaaag taatatcaat ttctttagta atgctataag
     8161 tagtttgctg attaaaatag tgttcaaaat attcttttct atcccaattt tctaattcaa
     8221 taatattaaa agtcatatat aacttcctcc taaattttaa atttttatat ttaggaggaa
     8281 taatcctctg attttttcat acgttatgtc acctcgtaaa tattaattat actgaattag
     8341 caatttttat caaataaaac ttattttact tccaaaacct aaattcacgt tgccaaaaat
     8401 caatctgctt ttgcaattgt ttttcgttcg cttttaaagt cgatttcatt aattcc
//
LOCUS       DADQRX010000304         5907 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.130, whole genome shotgun sequence.
ACCESSION   DADQRX010000304 DADQRX010000000
VERSION     DADQRX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.130"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2045
                     /locus_tag="J8V14_003045"
     CDS             <1..2045
                     /locus_tag="J8V14_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBA0923059.1"
                     /translation="IKQSQVDNNVGGGMIGAILYHFTYFLIAQPGSYLAAVLLLAGGA
                     FLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAAKEERMKQRAEAKEARRLAA
                     KEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPANLEK
                     PVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVDQSD
                     EYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIA
                     LALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRDVS
                     GRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNM
                     YNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLEN
                     GTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVI
                     TGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFI
                     SDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTAS
                     ISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            2275..3342
                     /gene="rlmN"
                     /locus_tag="J8V14_003046"
     CDS             2275..3342
                     /gene="rlmN"
                     /locus_tag="J8V14_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBA0923060.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3535..4638)
                     /locus_tag="J8V14_003047"
     CDS             complement(3535..4638)
                     /locus_tag="J8V14_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA0923061.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5191..5739
                     /gene="aac(6')"
                     /locus_tag="J8V14_003048"
     CDS             5191..5739
                     /gene="aac(6')"
                     /locus_tag="J8V14_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBA0923062.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1972 a         1023 c         1275 g         1637 t
ORIGIN      
        1 atataaaaca aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt
       61 atcattttac ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac
      121 tggcaggagg agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc
      181 aatcaattgg agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa
      241 agcaagcggc aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct
      301 tagctgcaaa agaagctgca gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga
      361 gccagcaaag ggaaaatact gctgatgaat ggcaggaacc agaaaataca gagccggagc
      421 agctaagttt tgttccaatc gacagttttc aagaaaatat ccatcctgcg aatctagaga
      481 aaccagtacc agatacacca aaacagacca atacagcaga aggtttttca gatgagttgc
      541 cagaggatga cggtacctca ttagaatttg agattgaagc agaacaggaa aatcaagact
      601 atgaattacc gacagttgat ttgcttgatt ctataccaac tgtggatcaa agtgatgagt
      661 ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg
      721 atgccaaggt cgtcaaagcc agcttaggac catccgtcac aaaattcgaa gtccagccgg
      781 ctgtaggggt aaaagtaagt aaaattgtga acttaacaga tgatatcgcg cttgctttag
      841 cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa atcactgatt ggtattgaag
      901 taccaaatgg aaagatcagt atggtttcct ttagagaaat catcgaagca caaccaaacc
      961 acccagataa attactagaa gtacctttag gacgtgatgt ttctggacgg gtacaaacgg
     1021 ctgatctttc gaagatgcct cacttactcg tggctggttc gactggtagt gggaagtcag
     1081 tagcaattaa tgggattatc acaagtatat taatgcgtgc taagccccat gaagtaaaac
     1141 tgatgatgat cgatccgaaa atggttgaat tgaatatgta caacggcata cctcatctgt
     1201 tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag
     1261 aaatggaaga acgatatgaa aaattcgcgg caaccggagt aagaaatatt tctggttaca
     1321 atgaatttgt tcaacagaaa aacttggaaa acggaacgaa acatccaacc ttaccattca
     1381 tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt agccagcaat gaagttgaag
     1441 atgcgattat tcgtttagcg caaatggctc gggcagcggg gattcatatg atcctagcta
     1501 ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa agcaaatgta ccttccagaa
     1561 tggcatttgc ggtatcaagc ggtacagatt ctcggacgat cattgattct aatggggcag
     1621 aaaagctctt agggcgagga gatatgctat tcttgccaat gggtgagaat aaaccgatcc
     1681 gtgtgcaagg agcattcatt tctgaccacg aagtagaacg ggtcgttcaa tttgttacag
     1741 atcagcaaga agctcattat gaagagaaaa tgatgccgac agatgaagtt gaaacgacag
     1801 gtgctccaga acagccacaa gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa
     1861 tgcaaacggc aagtatttcc cttctccaaa gaagatttcg gatcggttac aaccgagcag
     1921 cacgtttagt agatgaactg gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc
     1981 ctcggaaagt attcattgag caggaagaaa catcagaaac gttgccggac gataatgaca
     2041 tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga caaggcatga
     2101 tgataccaaa agaaaaaatg gtttttttcc atcttttcta atacggtatg atgtcttgtc
     2161 tttttagttt ttgtgagatt aaagttctat tcgatttatt tgtgaagtat tgcattaagg
     2221 aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa tactttggaa
     2281 aaacagtcca tttatggctt gacgaatgaa gaattaatca attggtttat agaaaacggc
     2341 gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat
     2401 ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga gcattttatc
     2461 atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg
     2521 tttgaactcc ctgataaaaa catgattgag actgtactga tgagacaaga atatggcttg
     2581 tcagtttgtg tgacgacaca agtaggatgt aatatcggct gtacgttttg tgcaagcggg
     2641 ctgctcaaaa agaaccgtga tctgactgct ggagaaattg ttgcacagat catgatggtc
     2701 caacattatt ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg
     2761 atcggtgagc catttgataa ttacgacaat gtaatggact ttttgcatat catcaatgat
     2821 gccaaaggat tggcaattgg tgctcggcac attacagttt ccactagtgg attagcacac
     2881 aaaatcaagg aattcgctaa taacggcttg caagtcaatc tggctatttc cttgcacgcg
     2941 ccgaataatg aggtgcgaac atcgatcatg cggatcaacc gaagtttccc aattgagaaa
     3001 ttgatggaag ctgtggatga gtatttagag aaaacaaacc gtcgaatcac gtttgaatat
     3061 atcatgttga atcaagtaaa tgatagacca gagcatgcgc aacaattagc agatctatta
     3121 aaagataaga aaaaattagc atatgtcaac ttgattccat ataatccagt cagtgaacat
     3181 gatcaatatt ctcgcagtcc aaaagctgat gtattgagat tttatgatgt attgaagaaa
     3241 aacggcgtaa actgtgtgat acgtaaagaa catggtacag atattgatgc agcttgcgga
     3301 caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt
     3361 ttcaattacc ataaaaggag cagttttctt aattaaggaa ctgttcaact taaataagag
     3421 aaaagattct atagcgaaag ctataaagct ttttttgaca agaaaaaccc tcatcaagca
     3481 aagaaatgtc ctcaatttga tgacagcctg cttgatgggg gttttagcaa ttatttaact
     3541 gattttctta tatccttgct ggctttcttg tgtagcatga taagtttcta cgtcaagaat
     3601 ggcctgttta aatttttctc gggtgatttc ataaggcaca tgtttaatat catttgttgc
     3661 catggcttta tctaagattg cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa
     3721 cgatactgga agttggttct ttttcataaa cacatagatt ttatcaaaca aatcgaactg
     3781 ctgatccatc atcaataagg tcagtacacc ataactgaca gatgctccgt gaaggtatcc
     3841 ctccattttt tctagaacag tcgtaccgta atagaatgag tgtgccatag cagaattata
     3901 gttattgtta actaaaacag aaaccaatcc ggtagtaaca atgatatccg aaacgacttg
     3961 ttcaattgca tatgagggac gatcgtcggc actatctttc aatgcttgcg taccataagt
     4021 caagagacga tcaatgcaca tttttccgat ttggattcct aatctatccg tgtagcctag
     4081 atcatcgcca cgagcagaaa aagtagattc gacttctttg gataaagcat cgccaatacc
     4141 tgcccataag tatttatcag gtgcttcggc aataattgct gtatcaatga aggtgtgtac
     4201 aggtggtttt gaaggaaaga agagtccgtc cattacgcct tcttcgttat agatgacaca
     4261 taccgcagtg acgggcgaac agttcgaacc aatagtggga aaggtgaaga ctggtttagc
     4321 tagtttttca ccaatgactt ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag
     4381 gacgtctgct ttttggacac tttcatgctg gatcaactgt tcaatatttc cataagttga
     4441 attacccccg taccagagac tggcgacaac ctctatttcc tgtgtcaaag ctacttttat
     4501 tttgttttct gcctttgaaa gtgcagtctt ccctccaatc agcactactt ttgtcccata
     4561 ttctttgacg attccatttg ctttttcata cgcatctgtt ccaatggtat aatttggtaa
     4621 gaaaacactt tgactcatat tcatttcctt ctttcttcgt tgaaacgggt taaaatttta
     4681 aagagtgatc gatctgactg cggctgtaga tggagtagag gtgaacctaa gacaggataa
     4741 gtgatgaata aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca
     4801 agtacaaaaa cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga
     4861 ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa ttaagattat tatatgaaaa
     4921 caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat
     4981 gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa
     5041 taaagtgttt ctgtataatt tttttatttt ataagtaaga taaataatga aagaatagag
     5101 atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt
     5161 tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat
     5221 ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga
     5281 gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg
     5341 gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt
     5401 tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga
     5461 ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt
     5521 acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt
     5581 gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta
     5641 ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg
     5701 atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa
     5761 gtaatcagct tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa
     5821 attccaactt atttccgaag aagctacttt tggaacaccg tgtaccagag gttgtgaaaa
     5881 agtcgcttag tttcgagtag taagaaa
//
LOCUS       DADQRX010000305         4802 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.147, whole genome shotgun sequence.
ACCESSION   DADQRX010000305 DADQRX010000000
VERSION     DADQRX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.147"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..785
                     /gene="vanR"
                     /locus_tag="J8V14_003049"
     CDS             90..785
                     /gene="vanR"
                     /locus_tag="J8V14_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBA0923063.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="J8V14_003050"
     CDS             763..1917
                     /locus_tag="J8V14_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBA0923064.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="J8V14_003051"
     CDS             2132..3100
                     /locus_tag="J8V14_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBA0923065.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="J8V14_003052"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="J8V14_003052"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBA0923066.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANIKKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="J8V14_003053"
     CDS             4130..4738
                     /locus_tag="J8V14_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBA0923067.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          921 c         1119 g         1280 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaaaaa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DADQRX010000306         4802 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.148, whole genome shotgun sequence.
ACCESSION   DADQRX010000306 DADQRX010000000
VERSION     DADQRX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.148"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..785
                     /gene="vanR"
                     /locus_tag="J8V14_003054"
     CDS             90..785
                     /gene="vanR"
                     /locus_tag="J8V14_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBA0923068.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="J8V14_003055"
     CDS             763..1917
                     /locus_tag="J8V14_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBA0923069.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="J8V14_003056"
     CDS             2132..3100
                     /locus_tag="J8V14_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBA0923070.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="J8V14_003057"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="J8V14_003057"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBA0923071.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="J8V14_003058"
     CDS             4130..4738
                     /locus_tag="J8V14_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBA0923072.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DADQRX010000307         3278 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.184, whole genome shotgun sequence.
ACCESSION   DADQRX010000307 DADQRX010000000
VERSION     DADQRX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.184"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            3..188
                     /locus_tag="J8V14_003059"
     CDS             3..188
                     /locus_tag="J8V14_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA0923073.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="J8V14_003060"
     CDS             203..1072
                     /locus_tag="J8V14_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBA0923074.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="J8V14_003061"
     CDS             1053..1787
                     /locus_tag="J8V14_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA0923075.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="J8V14_003062"
     CDS             1820..2728
                     /locus_tag="J8V14_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBA0923076.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="J8V14_003063"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="J8V14_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DADQRX010000308         1146 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.242, whole genome shotgun sequence.
ACCESSION   DADQRX010000308 DADQRX010000000
VERSION     DADQRX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.242"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..74
                     /locus_tag="J8V14_003064"
     CDS             9..74
                     /locus_tag="J8V14_003064"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBA0923077.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            199..936
                     /gene="erm(B)"
                     /locus_tag="J8V14_003065"
     CDS             199..936
                     /gene="erm(B)"
                     /locus_tag="J8V14_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBA0923078.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          420 a          199 c          185 g          342 t
ORIGIN      
        1 ggccttttat cttttacaca attatatgga ctttatcgaa acagactaaa aacagtgatt
       61 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      121 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat
      181 cacttcagga gagattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      241 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      301 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      361 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat
      421 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      481 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      541 tttgaaagcc gtgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      601 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      661 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      721 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      781 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      841 atgaaacacg ccaaagtaaa caatttaagt accattactt atgagcaagt attgtctatt
      901 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
      961 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1021 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1081 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1141 tgtatt
//
LOCUS       DADQRX010000309          635 bp    DNA     linear   BCT 03-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2215
            SAMN18324252-rid11861153.guided.269, whole genome shotgun sequence.
ACCESSION   DADQRX010000309 DADQRX010000000
VERSION     DADQRX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18324252
            Sequence Read Archive: SRR13984186
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000996267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000996267.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/02/2021 09:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 16 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18324252-rid11861153.guided.269"
                     /isolate="V2215"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2017-11-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="J8V14_003066"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="J8V14_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBA0923079.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          238 a           66 c          133 g          198 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagta
//
